The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure.

IF 5.6 2区 生物学 Q1 PLANT SCIENCES
Lei Ding, Maxime J Laurent, Thomas Milhiet, Stijn Aesaert, Mieke Van Lijsbettens, Laurens Pauwels, Hilde Nelissen, Dirk Inzé, François Chaumont
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引用次数: 0

Abstract

The plasma membrane aquaporin ZmPIP1;6 is expressed in maize stomatal complexes, with higher expression during the day than at night. To elucidate the role of ZmPIP1;6 in gas exchange and stomatal movement, it was expressed in maize (inbred line B104) under the control of p35S promoter (OE) or its native promoter fused with mYFP cDNA (mYFP-ZmPIP1;6). In stomatal complexes of the leaf mature zone, mYFP-ZmPIP1;6 showed higher expression in subsidiary cells than in guard cells, with light and dark treatments influencing its subcellular localization. Notably, ZmPIP1;6 internalization increased in dark conditions versus light. Stomatal opening was greater in ZmPIP1;6 OE than in wild type (WT), while closure exhibited greater sensitivity to elevated CO2 concentration or ABA treatment. Our finding revealed that reactive oxygen species (H2O2) was involved in ABA-induced stomatal closure, while ZmPIP1;6 was unable to facilitate H2O2 diffusion when expressed in yeast. Finally, ZmPIP1;6 OE and mYFP-ZmPIP1;6 transgenic plants exhibited higher abaxial stomatal density than WT. Overall, these results indicate that ZmPIP1;6 plays important roles in stomatal opening and CO2- and ABA-induced stomatal closure.

玉米水蒸发蛋白 ZmPIP1;6 可增强气孔开放以及 CO2- 和 ABA 诱导的气孔关闭。
质膜水通道蛋白ZmPIP1;6在玉米气孔复合体中表达,白天表达量高于夜间。为了阐明ZmPIP1;6在气体交换和气孔运动中的作用,在玉米(自交系B104)中,在p35S启动子(OE)或其与mYFP cDNA融合的天然启动子(mYFP-ZmPIP1;6)的控制下进行了表达。在叶片成熟区气孔复合体中,mYFP-ZmPIP1;6在辅助细胞中的表达高于在保卫细胞中的表达,光照和黑暗处理影响其亚细胞定位。值得注意的是,ZmPIP1;6内在化在黑暗条件下比在光照条件下增加。ZmPIP1;6 OE的气孔开度大于野生型(WT),而关闭度对CO2浓度升高或ABA处理更敏感。我们发现活性氧(H2O2)参与aba诱导的气孔关闭,而ZmPIP1;6在酵母中表达时不能促进H2O2的扩散。最后,ZmPIP1;6 OE和mYFP-ZmPIP1;6转基因植株的气孔密度高于WT。综上所述,这些结果表明ZmPIP1;6在气孔打开和CO2和aba诱导的气孔关闭中发挥了重要作用。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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