Journal of Fluorescence最新文献

{"title":"Luminescence Insights to Binding and Conformational Analysis of Graphene Oxide - Pepsin system: A Multi-Spectroscopic and In-Silico Approach.","authors":"Piyush Verma, Lajpreet Kaur, Rajan Patel, Himanshu Ojha, Mallika Pathak","doi":"10.1007/s10895-025-04530-5","DOIUrl":"https://doi.org/10.1007/s10895-025-04530-5","url":null,"abstract":"<p><p>Pepsin is a key proteolytic enzyme involved in the gastric digestion. Graphene oxide (GO) despite the promising biomedical applications, lacks consensus for its use in medicines. One of the leading causes in this respect is the limited understanding of its interaction with model proteins. In this background, the present study investigates the biophysical interaction of GO with pepsin using UV-Vis, Fluorescence, FT-IR spectroscopy, and in-silico modelling. UV-Vis spectra of pepsin revealed slight hypsochromic and hyperchromic shift in the presence of GO indicating alteration in pepsin's structure corroborated by fluorescence quenching study. Further analysis of fluorescence data shows a decrease in Stern-Volmer quenching constant, K_sv (order of 10⁵ L g^-1) decreases with increasing temperature that indicates static quenching which is essentially due to complexation. The complexation was further characterized in terms of association constant (non-covalent interactions) and the equivalent number of binding sites (close to one). Thermodynamic analysis suggested the binding interaction is essentially entropy driven. Synchronous fluorescence spectra suggested microenvironment changes for both the tryptophan and tyrosine residues in the binding sites. Detailed secondary structure study of pepsin (native and in presence of GO) using ATR-FTIR confirmed significant changes in the protein conformation (α-sheet, random coil, and β-helix) upon interaction with GO. Molecular docking suggested the involvement of H-bonding and π- π interactions within the GO-pepsin system with a binding score of -11.47 kcal/mol. DLS study indicated concentration dependent increase in hydrodynamic diameter of pepsin in presence of GO. Finally, physiological activity of pepsin was found decreased (up to 54%) upon incubation with GO suggested change in its activity. This work provides valuable insights into the binding mechanism and interaction of GO with pepsin with potential implications for the development of an oral GO based formulations in the near future.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perimidine Derivative as a Colorimetric/fluorescence turn-on Sensor for Fe³⁺: Smartphone-assisted Sensing and Quantification of Fe³.","authors":"Ruhi Mehta, Rajbir Kaur, Navneet Khetrapal, Sanjay Kumar","doi":"10.1007/s10895-025-04537-y","DOIUrl":"https://doi.org/10.1007/s10895-025-04537-y","url":null,"abstract":"<p><p>A novel perimidine derivative: 2-(isoquinolin-1-yl)-1H-perimidine (sensor 1) was synthesized via a facile, one-step reaction between DAN (1,8 diaminonaphthalene) and 2- quinolinecarboxaldehyde. The as-synthesised sensor 1 was found to be stable under a wide pH range of 2-12, and it behaved as a colorimetric as well as fluorescence turn-on sensor for Fe³⁺ with a response range varying from 0 to 200 µM. The fluorescence titration profile was used to calculate the binding constant as 1.3 × 10⁴ M^-1 and the LOD was as low as 7.4 nM. Additionally, the interference studies revealed the capability of 1.Fe³⁺ complex to sense Cu²⁺, which was accompanied by the fluorescence turn-off response. The binding constant and detection limit of 1.Fe³⁺ complex for Cu²⁺ were calculated to be 1.44 × 10³ M and 1.13 × 10^-7 M, respectively. The Fe³⁺ induced fluorescence enhancement at different concentrations was also recognized via smartphone, which could benefit in resource-limited areas and could be used as an alternative for complex instrumentation.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmentally friendly synthesis of carbon quantum dots (CQDs) to enhance photocatalytic activity for the photodegradation of various organic dyes and the evolution of hydrogen and oxygen.","authors":"Saeedeh Rastgar, Noureddine Elboughdiri","doi":"10.1007/s10895-025-04405-9","DOIUrl":"https://doi.org/10.1007/s10895-025-04405-9","url":null,"abstract":"<p><p>This review delivers a focused and critical evaluation of recent progress in the green synthesis of carbon quantum dots (CQDs), with particular attention to state-of-the-art approaches utilizing renewable biomass as precursors. The main objective is to systematically examine innovative, environmentally friendly methods and clarify their direct influence on the core properties and photocatalytic performance of CQDs. The novelty of this review stems from its comprehensive comparison of green synthetic pathways, revealing how specific processes determine key structural, optical, and electronic attributes of the resulting CQDs. These intrinsic properties, in turn, enable significantly higher photocatalytic efficiency. Notable advancements highlighted include: (i) the enhanced stability and broadened visible light absorption achieved through green synthesis; (ii) improved photogenerated charge carrier separation; and (iii) superior rates of organic pollutant degradation (e.g., methylene blue, rhodamine B, phenol) and effective solar-driven hydrogen and oxygen evolution. Additionally, the review addresses how fine-tuning surface functionalities and synthesis parameters at the molecular level can optimize both catalytic reactivity and selectivity. By integrating and critically synthesizing the latest research, this review not only underscores the pivotal role of sustainable CQD fabrication for environmental remediation and clean energy generation, but also identifies major challenges and outlines future research directions. These insights are intended to guide the rational design of high-performance, eco-friendly photocatalytic nanomaterials in line with green chemistry principles.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrot-Derived Nitrogen-Doped Dual-Emission Carbon Dots for Ratiometric Detection of Tetracycline and Oxytetracycline.","authors":"Tengzhi Zhao, Zhaofeng Wang, Runke Zhang, Hui Xu, Hua Wang, Ying Yu","doi":"10.1007/s10895-025-04531-4","DOIUrl":"https://doi.org/10.1007/s10895-025-04531-4","url":null,"abstract":"<p><p>The antibiotic contamination in aquatic environments, particularly in aquaculture systems, poses substantial risks to ecological balance and human health. To address this issue, we engineered a novel ratiometric fluorescent probe utilizing dual-emission carbon dots (D-CDs) synthesized from sustainable biomass carrot and nitrogen-rich precursors (melamine and o-phenylenediamine) through an efficient one-pot hydrothermal approach. The D-CDs exhibited dual emission peaks at 425nm and 540 nm under 370nm excitation. The fluorescence intensity ratio (F₅₄₀/F₄₂₅) demonstrated linear response toward tetracycline (TC) and oxytetracycline (OTC), with wider linear detection ranges of 0.027-133.3 µM for TC and 0.027-233.3 µM for OTC, respectively. Low detection limits (17.7 nM for TC and 22.04 nM for OTC) were also achieved. Mechanistic studies revealed that quenching resulted from static quenching and inner filter effects. The probe showed exceptional stability under high ionic strength and long storage conditions. Real-sample monitoring in lake water and aquaculture samples yielded recovery rates of 90.4-103.5% with RSD < 6.88%, which was validated by high-performance liquid chromatography (HPLC), demonstrating the promising potential of this probe.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Benzothiazole Derived Fluorescent Sensor for Real-time Detection of Aluminums Ion and Application in Real Samples and Living Cells.","authors":"Liangping Li, Guiying Hu, Wen Qin, Zhiqian Liang, Xuerong Yang, Zhiyong Xing","doi":"10.1007/s10895-025-04468-8","DOIUrl":"https://doi.org/10.1007/s10895-025-04468-8","url":null,"abstract":"<p><p>Excessive aluminum ions (Al³⁺) contaminate environmental water bodies, and once they remain in the human body, they will pose a threat to human health. In this paper, a benzothiazole-based fluorescent sensor named BHMH for detecting Al³⁺ was successfully synthesized and comprehensively characterized. It exhibited remarkable selectivity and sensitivity, manifested by a substantial fluorescence augmentation and a discernible color alteration in the presence of Al³⁺. The job plot, ¹H NMR titration and HRMS demonstrated that the BHMH and Al³⁺ complex exhibited a 1:1 stoichiometric binding affinity. Furthermore, the limit of detection (LOD) for Al³⁺ was calculated as 4 × 10^-8 M. Additionally, BHMH demonstrated successful application in the precise detection of Al³⁺ across diverse aqueous samples, test papers and cell imaging within living HSC cells.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and Recognition Performance of Fleroxacin Ratio Fluorescent Paper-based Sensor Based On Molecularly Imprinted Polymer.","authors":"Xiaoling Luo, Zheng Liu, Pintian Zou, Yiju Lv, Xiangfei Kong, Lei Cao, Guo-Cheng Han","doi":"10.1007/s10895-025-04552-z","DOIUrl":"https://doi.org/10.1007/s10895-025-04552-z","url":null,"abstract":"<p><p>The pervasive concern regarding veterinary drug residues in food necessitates advanced detection solutions, particularly addressing limitations of conventional methods reliant on large-scale instrumentation that incur prolonged analysis duration, complex sample preparation, and lack of real-time on-site capability. A portable \"single response-on\" molecularly imprinted ratiometric fluorescent paper-based sensor was developed for quantifying fleroxacin (FLX) residues in animal-derived foods, wherein B, N-co-doped MXene quantum dot (B, N-MQD) was synthesized and combined with BCP-Eu as dual-emission fluorophores, while FLX- molecularly imprinted polymer (FLX-MIP) was engineered using functionalized Nano-SiO₂ as the carrier. Concentration-dependent fluorescence enhancement at 574 nm was exhibited with invariant reference signal at 411 nm, achieving a 36-fold lower detection limit (0.49 µM) versus the non-imprinted probe (17.78 µM) and linear response across 25.0 ~ 200.0 µM. When integrated with smartphone visualization, 25.0 ~ 350.0 µM linearity, 4.78 µM detection limit, and 89.80 ~ 96.44% recoveries were demonstrated in real samples, validating reliability for on-site screening. * Corresponding authors. E-mail: lisa4.6@163.com(Z. Liu), peter.zou@rid-lab.cn(P.T. Zou), hangc81@guet.edu.cn(G.-C. Han).</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exclusive Detection of Ni²⁺, Cu²⁺, Zn²⁺, and CO₃^2- Ions Using a Novel Schiff Base.","authors":"Siddharth Gautam, Nitish Kumar Singh, Nancy Gupta, Md Najbul Hoque","doi":"10.1007/s10895-025-04521-6","DOIUrl":"https://doi.org/10.1007/s10895-025-04521-6","url":null,"abstract":"<p><p>This study reports the synthesis, characterization, and multifunctional sensing capabilities of a novel quinoline-based Schiff base ligand (L), designed for selective and sensitive detection of Ni²⁺, Cu²⁺, Zn²⁺ ions, and CO₃²⁻ anions. L exhibits distinct colorimetric responses visible to the naked eye-pale yellow to amber red for Ni²⁺, caramel brown for Cu²⁺, and canary yellow for Zn²⁺-enabling efficient and straightforward detection. Fluorescence studies reveal a selective green fluorescence \"turn-on\" response for Zn²⁺, complemented by fluorescence quenching in the presence of CO₃²⁻, demonstrating the ligand's reusability and robustness. Moreover, the fluorescent quenching of L-Zn by CO₃²⁻ enabled the detection of CO₃²⁻ using the Stern-Volmer plot, with a limit of detection (LOD), the limit of quantification (LOQ), and rate constant (K) for CO₃²⁻ calculated to be 3.594 µM, 10.891 µM, and 0.64 mol^-1L, respectively. Furthermore, a Job's plot experiment was conducted to determine the precise stoichiometric ratio of complex formation, revealing a 1:2 (ML₂) adduct between M and L, which was further confirmed by ESI-MS spectra and DFT calculation. Comprehensive analyses, including FTIR, ESI-MS, ¹H and ¹³C NMR, UV-Visible, fluorescence spectroscopy, and DFT calculations, elucidate the electronic properties, binding, and sensing mechanism of L. NBO, MEP mapping and HOMO-LUMO analysis further highlight nucleophilic and electrophilic reactive sites, confirming the L's suitability for selective coordination with metal ions. Compared to existing sensing systems, L offers competitive or superior detection limits, high selectivity, and visual responsiveness, making it a potential candidate for practical applications in metal ion sensing.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Green Synthesised B/nZVCu-M On-Off Fluorescence Sensor for Dopamine Detection and its Reusability.","authors":"Dipanwita Basak, Sushmita Gajurel, Aseem Vashisht, Hemaprobha Saikia","doi":"10.1007/s10895-025-04544-z","DOIUrl":"https://doi.org/10.1007/s10895-025-04544-z","url":null,"abstract":"<p><p>The real-time and selective detection of dopamine (DA) in complex biological systems remains a critical challenge due to its low physiological concentrations and interference from structurally similar biomolecules such as ascorbic acid and uric acid. Traditional analytical techniques often fall short in terms of specificity, cost-effectiveness, and ease of deployment in biological matrices. To address this gap, we developed a highly selective fluorescent nanosensor based on bentonite-supported Cu-based bimetallic nanoparticles (B/nZVCu-Ni/Ag), synthesized via a green route using Lawsonia inermis extract. The phytogenic synthesis facilitated the reduction and stabilization of the nanoparticles, yielding well-dispersed crystalline domains (10-13 nm) anchored uniformly on the bentonite layered sheets. Comprehensive characterization through PXRD, FTIR, TEM, UV-Vis, and fluorescence spectroscopy confirmed the formation and functional integration of the nanoparticles. Among the two variants, the B/nZVCu-Ni (D1) probe exhibited superior fluorescence enhancement upon interaction with DA, achieving a detection limit as low as 5.13 nM and a high binding constant (K = 1.47 × 10⁵ M⁻¹). The sensor demonstrated excellent selectivity, with negligible response to common interfering agents, and displayed a robust reversible on-off fluorescence behavior upon sequential addition and removal of DA using EDTA^2- , confirming dynamic binding reversibility. These results establish the B/nZVCu-M nanoprobes, particularly D1, as promising candidates for ultrasensitive, interference-resistant, and reusable fluorescence-based detection of dopamine in synthetic urine sample.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Benzimidazole-Based Schiff Bases as Fluorescent Probes for the Sensitive and Selective Detection of Cu²⁺ Ions in Water Samples.","authors":"Zafer Ocak, Abidin Gümrükçüoğlu, Miraç Ocak, Ümmühan Turgut Ocak","doi":"10.1007/s10895-025-04543-0","DOIUrl":"https://doi.org/10.1007/s10895-025-04543-0","url":null,"abstract":"<p><p>A new spectrofluorimetric method has been developed for the selective detection of Cu²⁺ ions based on a benzimidazole-containing Schiff base ligand. In this study, three structurally related Schiff bases (L1, L2, and L3) were synthesized and comparatively investigated for their fluorescence response in the presence of various cations. Among them, only L3 bearing ortho-hydroxyl groups on the aromatic rings exhibited a notable fluorescence quenching effect upon interaction with Cu²⁺, while L1 (para-hydroxyl substituted) and L2 (N, N-dimethylamino substituted) showed limited or no selectivity. The structure-function relationship revealed that the spatial orientation of donor groups plays a key role in metal coordination and signal transduction. The proposed method good sensitivity and selectivity toward Cu²⁺, with a linear response in the concentration range of 0-32 µg/L and a low detection limit of 0.98 µg/L, enabling quantification at relatively low concentrations. Furthermore, the method was tested with tap water samples. This work offers a potentially useful approach for Cu²⁺ determination but also highlights the importance of ligand geometry in fluorescent probe design.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polysaccharide-Based Fluorescent Cu²⁺-Responsive Nanocarrier for Dendrobium-Induced Pyroptosis and Inhibition of Non-Small Cell Lung Cancer.","authors":"Chunmin Dong, Yue Wang","doi":"10.1007/s10895-025-04548-9","DOIUrl":"https://doi.org/10.1007/s10895-025-04548-9","url":null,"abstract":"<p><p>Non-small cell lung cancer (NSCLC) remains one of the most lethal malignancies worldwide, highlighting the urgent need for the development of novel multifunctional therapeutic strategies. In this study, a bioinspired nanocomposite drug delivery system was designed and constructed by covalently modifying propylene glycol alginate (PGA) with a microbial-derived coumarin compound (Compound 1) and a fluorinated small molecule (Compound 2), followed by assembly with the silane-based crosslinker ATPMS. The system was subsequently loaded with Dendrobium extract to produce the final nanocomposite material, 2-PGA-1-ATPMS@Dendrobium. This platform exhibited excellent biocompatibility, enhanced cellular uptake, and significant anti-proliferative effects against NSCLC cells. Mechanistic investigations revealed that the nanomaterial induced tumor cell pyroptosis by upregulating the expression of Caspase-1 and GSDMD and promoting the transcription of pro-inflammatory cytokines. Moreover, the nanocomposite demonstrated ultra-sensitive ratiometric fluorescence detection of Cu²⁺ ions, with a detection limit as low as 0.068 nM. Given the critical role of copper ions in inducing cuproptosis and their involvement in tumor progression, this dual-functional nanoplatform presents promising potential for both early diagnosis and targeted treatment of NSCLC.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}