Journal of Fluorescence最新文献

{"title":"Enhanced Fluorescence and Fenton-reaction in Peroxidase Nanozyme of Functionalized Fe, N, S-Carbon Dots: H₂O₂-Induced Surface Oxidation and Detection using Conventional Turn-off and Smartphone-based Colorimetric Methods.","authors":"Shaimaa Abubakr Abdalla, Shilan A Fatah, Sameera Sh Mohammed Ameen, Khalid M Omer","doi":"10.1007/s10895-026-04971-6","DOIUrl":"https://doi.org/10.1007/s10895-026-04971-6","url":null,"abstract":"<p><p>Peroxidase nanozymes and fluorophores have attracted considerable attention in catalysis and luminescence-based sensing applications. In this study, novel class of iron-, nitrogen-, and sulfur-functionalized carbon dots (Fe/N/S-CDs) featuring with inherent fluorescence and peroxidase-like activity were developed successfully via a facile hydrothermal approach. The Fe/N/S-CDs displayed strong blue fluorescence under UV irradiation with a high quantum yield of 44.5%. The incorporation of Fe active centers within the carbon framework and surface structure endowed Fe/N/S-CDs with efficient peroxidase-like activity toward hydrogen peroxide (H₂O₂) through a Fenton-like reaction, producing hydroxyl radicals (•OH). The generated •OH radicals induced surface oxidation of Fe/N/S-CDs, resulting in fluorescence quenching and enabling a selective \"on-off\" sensing mechanism. Structural and spectroscopic analyses confirmed H₂O₂-induced surface oxidation of Fe/N/S-CDs, accompanied by increased oxygen-containing functional groups, altered electronic structure, and sustained Fe²⁺/Fe³⁺ redox cycling. Based on this strategy, a dual-mode sensing platform integrating fluorometric detection and smartphone-assisted colorimetric analysis was developed for H₂O₂ monitoring. The proposed sensor achieved low detection limits of 3.55 µM and 8.99 µM for fluorometric and smartphone-based colorimetric detection, respectively. Wide linear ranges of 4-5000 µM for fluorometric analysis and 30-1000 µM for smartphone-based detection were obtained. The practical applicability of the sensing platform was evaluated using commercial and medical H₂O₂ samples, demonstrating excellent recovery values ranging from 98.9% to 104.8% with low RSDs. The integration of functionalized Fe/N/S-CD nanozymes with smartphone-based analysis establishes a robust dual-mode sensing platform that paves the way for accessible, reliable, and real-time chemical sensing in environmental, biomedical, and food safety applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 3,4-Ethylenedioxythiophene-Derived Fluorescent Probe for Highly Selective Cadaverine Detection and Food Freshness Monitoring.","authors":"Guolong Su, Xin Yu, Haitao Zhang, Xiao Chen, Caixu He, Xiuzhong Zhu, Shaoming Wang","doi":"10.1007/s10895-026-04978-z","DOIUrl":"https://doi.org/10.1007/s10895-026-04978-z","url":null,"abstract":"<p><p>Biogenic amines are important indicators of food spoilage and freshness. In this study, four 3,4-Ethylenedioxythiophene (EDOT) derivatives, EDOT-EN₁, EDOT-EN₂, EDOT-EN₃, and EDOT-EN₄, were synthesized as fluorescent probes for the detection of biogenic amines. The four fluorescent probes were prepared by introducing different substituents based on 3,4-ethylenedioxythiophene. Comparison of their photophysical properties showed that EDOT-EN₁ exhibited the most favorable photophysical and sensing performance among the four probes. EDOT-EN₃ and EDOT-EN₄ exhibited substantially weaker fluorescence than EDOT-EN₁ and EDOT-EN₂, resulting in insufficient signal contrast for quantitative sensing. Therefore, subsequent optimization focused on EDOT-EN₁ and EDOT-EN₂. EDOT-EN₂ showed a response time comparable to that of EDOT-EN₁. However, during the titration process, the concentration-dependent fluorescence response was less regular and less sensitive, making it less suitable for quantitative analysis, and its titration upper limit was approximately 1.5 times higher than that of EDOT-EN₁. Therefore, EDOT-EN₁ was selected for further investigation. The probe enables the detection of 1,5-pentanediamine (Cad) due to the aza-Michael addition reaction between EDOT-EN₁ and Cad, resulting in Schiff-base products. Furthermore, EDOT-EN₁ was immobilized on filter paper to fabricate portable fluorescent test strips for monitoring the freshness of shrimp and fish. Additionally, an electrospun film doped with EDOT-EN₁ was prepared, which can selectively detect Cad vapor.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Blue-Green Emitting Oxadiazole: Photophysical, Solvatochromic, and FRET Studies with Core-Shell Quantum Dots.","authors":"Sushma Katti, Amith Yadav H J, G H Pujar, S R Inamdar, Narahari Deshapande, Vinay Parol, Vinuta J B, R Vijay Kumar, Veena V S","doi":"10.1007/s10895-026-04979-y","DOIUrl":"https://doi.org/10.1007/s10895-026-04979-y","url":null,"abstract":"<p><p>The development of blue-green emitting organic chromophores with excellent optoelectronic properties remains an important issue. In this context, the newly synthesized oxadiazole-based blue-green emitting chromophore, 2-(4-(2-(anthracen-10-yl)vinyl)phenyl)-5-(4-tert-butylphenyl)-1,3,4-oxadiazole (AVBO), was accomplished via the donor-π-acceptor (D- π-A) strategy using a Wittig reaction. The integration of aryl-heteroaryl groups and the oxadiazole group was utilized to obtain efficient blue-green emission with excellent optical properties. AVBO exhibited stable and efficient PL in the range of 485-510 nm across diverse solvent environments. Solvatochromic analysis using multiple correlations (Lippert-Mataga, Bakhshiev, Kawski-Chamma-Viallet, and Reichardt) revealed a significantly higher excited-state dipole moment compared to the ground state ([Formula: see text]>[Formula: see text]), with a parallel orientation ([Formula: see text]). The values calculated using these empirical equations were compared with those calculated computationally. Kamlet-Taft and Catalán MLR analyses further identified dominant non-specific solvent interactions ([Formula: see text]) alongside specific hydrogen-bonding donor contributions. To explore its potential in optoelectronic applications, AVBO was integrated into hybrid systems with ZnSe/ZnS core-shell quantum dots (7-8 nm). This assembly functioned as an efficient FRET mechanism, reaching an efficiency of 88% with Förster radii of 6.14-6.16 nm. The quenching kinetics, validated by Stern-Volmer plots, showed both static and dynamic components with super-diffusion-limited rate constants (k_q ≈ 10¹⁴ /MS). Time-resolved photoluminescence (TRPL) confirmed energy transfer via dipole-dipole interactions. These findings highlight the AVBO-QDs hybrid as a promising, cadmium-free, and tunable emitter for light harvesting, photosensitization, and bio-sensing applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering a Synthetic Fluorogenic RNA Switch for Oxidized Guanine.","authors":"Micaela Belleperche, Kristina Gremi, Isabeli P Maurutto, Emma Thauvin, Adriana A Bacon, Jiawen Liu, Maureen McKeague","doi":"10.1007/s10895-026-04976-1","DOIUrl":"https://doi.org/10.1007/s10895-026-04976-1","url":null,"abstract":"<p><p>Reactive oxygen species generate a family of oxidized guanine species that serve as important biomarkers of oxidative damage. Here, we report the development of a fluorogenic RNA switch that selectively recognizes oxidized guanine species. We first systematically characterized an oxidized guanine RNA aptamer, defining its minimum functional binding domain and molecular recognition profile. Structural minimization produced a compact aptamer with higher affinity than the full-length sequence that retained broad recognition of structurally diverse oxidized guanine species while discriminating against their unmodified counterparts. We subsequently incorporated the optimized aptamer into fluorogenic RNA switch architectures, revealing that fluorescence activation required iterative optimization of aptamer topology, scaffold architecture, and communication-linker design. The resulting fluorogenic RNA switch exhibited target-dependent fluorescence activation while retaining the aptamer's molecular recognition profile. This work establishes a fluorogenic RNA switch for oxidized guanine species and highlights the importance of systematic aptamer characterization and optimization in fluorogenic RNA switch development.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Dicyanovinyl-Substituted D-π-A Type Chemosensor for Selective and Sensitive Detection of Cyanide Ions in H₂O/DMSO (1:9, v/v) Medium.","authors":"Murat Acar","doi":"10.1007/s10895-026-04974-3","DOIUrl":"https://doi.org/10.1007/s10895-026-04974-3","url":null,"abstract":"<p><p>The detection of hazardous analytes such as CN⁻, N₂H₄, and ClO⁻ is of great importance due to their potential risks to human health and the environment. Although numerous fluorescent sensors have been developed for the determination of these species, most studies have focused on individual analytes; conversely, the simultaneous evaluation of CN⁻, N₂H₄, and ClO⁻ considering them as potential interfering species has received relatively limited attention. Therefore, in this study, a dicyanovinyl-substituted D-π-A type organic material (TPA-Th-DCV) was developed as a fluorescent sensor exhibiting a rapid response, high selectivity, and excellent sensitivity toward cyanide ions (CN⁻) even in the presence of other anions (N₂H₄, ClO⁻ and etc.). The photophysical behavior of TPA-Th-DCV was significantly altered by the nucleophilic addition of CN⁻ to its vinyl group, which disrupted the intramolecular charge transfer (ICT) process. Fluorescence measurements revealed a remarkably low limit of detection (LOD) of 48 nM. The sensing mechanism of TPA-Th-DCV toward CN⁻ was elucidated through NMR spectroscopy, mass spectrometry, and DFT/TDDFT calculations. Moreover, the turn-on fluorescence response of TPA-Th-DCV enabled the successful detection of cyanide in common food samples, demonstrating its strong potential for real-world environmental and food safety monitoring applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DFT and TD-DFT Investigation of Mg Adsorption, Electronic Structure, and Optical Properties of Finite M₂B₂ MBene Quantum Dots (M = Cr, Fe, Mn, Sc, Ti, and V).","authors":"Hazem Abdelsalam, Mahmoud A S Sakr, Ghada M Abdelrazek, Manal M Alkhamisi, Omar H Abd-Elkader, Qinfang Zhang","doi":"10.1007/s10895-026-04946-7","DOIUrl":"https://doi.org/10.1007/s10895-026-04946-7","url":null,"abstract":"<p><p>The electronic structure, Mg adsorption behavior, and optical response of M₂B₂ MBene quantum dots (M = Cr, Fe, Mn, Sc, Ti, and V) were systematically investigated using density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations to evaluate their potential as anode materials for magnesium-ion batteries. The calculated adsorption energies demonstrate that the transition metal species strongly influences Mg binding, with V₂B₂ and Fe₂B₂ exhibiting the most favourable adsorption energies of - 1.44 and - 1.31 eV, respectively, accompanied by open-circuit voltages of 0.72 and 0.65 V. Among the investigated systems, V₂B₂ delivers the highest theoretical specific capacity of 780.93 mAh g⁻¹ while maintaining stable Mg adsorption energies ranging from - 0.94 to - 1.09 eV during successive Mg loading, indicating favorable electrochemical reversibility. Frontier molecular orbital, density of states, and charge-transfer analyses reveal pronounced Mg-V orbital hybridization and reduced HOMO-LUMO energy gaps upon Mg adsorption, leading to enhanced electronic conductivity. Furthermore, TD-DFT calculations predict tunable optical absorption extending from the near-infrared to the mid-infrared region with progressive red shifts as the Mg concentration increases. These findings provide molecular-level insight into the structure-property relationships governing Mg storage in MBene quantum dots and identify V₂B₂ as a promising candidate for next-generation magnesium-ion battery anodes.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Fe₃O₄@Au-Based Fluorescence-Colorimetric Aptasensor for Acetamiprid Assay.","authors":"Qing Bao, Yu Zhang, Qingqing Xu, Yingying Lun, Jianguo Feng, Sa Dong","doi":"10.1007/s10895-026-04973-4","DOIUrl":"https://doi.org/10.1007/s10895-026-04973-4","url":null,"abstract":"<p><p>Acetamiprid (ACE) residues in vegetables from long-term pesticide use pose potential toxicity risks to human health. Therefore, simple and accurate methods for ACE detection are essential for food safety. In this study, a dual-mode aptasensor was developed based on the specific recognition of fluorescent aptamers (Apts-FAM) combined with the fluorescence quenching and peroxidase-mimicking catalytic properties of Fe₃O₄@Au nanocomposites. The optimal conditions (quenching concentration/time, ACE incubation time) were determined. Under optimized conditions, a linear relationship between ACE concentration (0.5-10 µg/mL) and both fluorescence intensity change (ΔF) and absorbance (A) was established. The fluorescence calibration curve was ΔF = 373.621 C + 5406.812 (R² = 0.9940) with a detection limit (LOD) of 0.416 µg/mL(0.25 mg/kg). The colorimetric response was A = 0.03344 C + 0.10842 (R² = 0.9965) with an LOD of 0.485 µg/mL(0.29 mg/kg). Specificity tests showed no significant cross-reactivity with four structural analogs (dinotefuran, thiamethoxam, imidacloprid, nitenpyram). Reproducibility tests (five replicates at 10 µg/mL ACE) gave relative standard deviations below 8% for both detection modes. In real vegetable samples (Chinese cabbage and cucumber), recovery rates for fluorescence signals ranged from 84.07% to 95.83% (CV ≤ 9.56%), and for colorimetric signals from 80.56% to 91.81% (CV ≤ 7.18%), confirming strong anti-matrix interference capability. These results demonstrate that the dual-signal aptasensor offers good sensitivity and accuracy, making it suitable for practical ACE residue analysis in vegetables.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blue-emissive glyoxal monohydrates via selenium-nitric acid oxidation of methyl ketones.","authors":"Siva Dakshayani Vadanapalli, Sai Teja Talari, Mohan Das Dasappa, Chinna Gangi Reddy Nallagondu","doi":"10.1007/s10895-026-04940-z","DOIUrl":"https://doi.org/10.1007/s10895-026-04940-z","url":null,"abstract":"<p><p>An efficient, practical, economically and industrially viable synthetic route has been demonstrated for the synthesis of a library of arylglyoxal monohydrates (AGMHs) (2) in good to quantitative yields (90-99%) from the oxidation of a wide variety of aryl/heteroaryl methyl ketones (1) using a mixture of selenium metal and nitric acid in 1,4-dioxane. Easy to perform, broad substrate scope, good functional group tolerance, shorter reaction times, simple work-up procedure, gram-scale feasibility and products do not require column chromatography purification are the attractive features of the present reported methodology. Most importantly, the selenium metal is recovered simply by filtration and reused successfully for subsequent reaction cycles. Hence, the established procedure for the oxidation of aryl/heteroaryl methyl ketones (1) is a practical and sustainable alternative protocol to the reported methods. The photophysical properties of the synthesized AGMHs (2) were systematically investigated. The results demonstrated that these compounds exhibit blue light emission spanning the purplish-blue to bluish-green spectral region. Notably, compound 2o showed intense blue emission with CIE chromaticity coordinates of x = 0.14 and y = 0.08, closely matching as per the blue color standards of National Television System Committee (NTSC). These results reveal that AGMHs (2) may serve as potential candidates for future studies in optoelectronics.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Chapter for the Journal of Fluorescence: A Welcome Message from the Incoming Editor-in-Chief.","authors":"Carlos Lodeiro","doi":"10.1007/s10895-026-04969-0","DOIUrl":"https://doi.org/10.1007/s10895-026-04969-0","url":null,"abstract":"","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Curcumin-Based Turn-on Fluorescent Probe for Superoxide Anion in Living Cells.","authors":"Yamei Li, Qinghua Zhu, Jing Nie, Shansi Wei, Dongbo Wang, Saipeng Huang, Jiyong Wu","doi":"10.1007/s10895-026-04972-5","DOIUrl":"https://doi.org/10.1007/s10895-026-04972-5","url":null,"abstract":"<p><p>Superoxide (O₂^•-) plays a pivotal role in diverse physiological and pathological processes, a thorough understanding of its molecular mechanisms relies on dependable detection techniques. In this study, we designed and synthesized DFCD, a novel curcumin-based fluorescent probe with a switching mechanism, for the highly selective and sensitive detection of O₂^•-. By employing diphenylphosphinyl chloride as the reactive group, DFCD operates through a photoinduced electron transfer (PET) mechanism and exhibits significant fluorescence enhancement at 574 nm upon reaction with superoxide anion. The probe demonstrates a detection limit of 0.11 µM, excellent selectivity over competing reactive species and ions, good stability at physiological pH, and minimal cytotoxicity. Live-cell imaging confirms that DFCD is capable of monitoring endogenous superoxide anion dynamics in HeLa cells under various stimuli. Furthermore, DFCD proves highly effective for imaging macrophage inflammatory responses, providing a robust tool for studying superoxide-driven oxidative stress in inflammatory models. Collectively, these findings establish DFCD as a valuable imaging probe for studying superoxide-related oxidative stress at the cellular level.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}