Journal of Fluorescence最新文献

{"title":"A Curcumin-Based Turn-on Fluorescent Probe for Superoxide Anion in Living Cells.","authors":"Yamei Li, Qinghua Zhu, Jing Nie, Shansi Wei, Dongbo Wang, Saipeng Huang, Jiyong Wu","doi":"10.1007/s10895-026-04972-5","DOIUrl":"https://doi.org/10.1007/s10895-026-04972-5","url":null,"abstract":"<p><p>Superoxide (O₂^•-) plays a pivotal role in diverse physiological and pathological processes, a thorough understanding of its molecular mechanisms relies on dependable detection techniques. In this study, we designed and synthesized DFCD, a novel curcumin-based fluorescent probe with a switching mechanism, for the highly selective and sensitive detection of O₂^•-. By employing diphenylphosphinyl chloride as the reactive group, DFCD operates through a photoinduced electron transfer (PET) mechanism and exhibits significant fluorescence enhancement at 574 nm upon reaction with superoxide anion. The probe demonstrates a detection limit of 0.11 µM, excellent selectivity over competing reactive species and ions, good stability at physiological pH, and minimal cytotoxicity. Live-cell imaging confirms that DFCD is capable of monitoring endogenous superoxide anion dynamics in HeLa cells under various stimuli. Furthermore, DFCD proves highly effective for imaging macrophage inflammatory responses, providing a robust tool for studying superoxide-driven oxidative stress in inflammatory models. Collectively, these findings establish DFCD as a valuable imaging probe for studying superoxide-related oxidative stress at the cellular level.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crystal Structure, Photophysical Properties, Third-Order Nonlinear Optical Response, Density Functional Theory, and Molecular Docking Studies of a Non-Centrosymmetric D-π-A Conjugated Dienone.","authors":"S Raghavendra, Guddekoppa S Ananthnag, Sampath Chinnam, Itte Pushpavathi, G Vinitha","doi":"10.1007/s10895-026-04968-1","DOIUrl":"https://doi.org/10.1007/s10895-026-04968-1","url":null,"abstract":"<p><p>A donor-π-acceptor conjugated dienone, (2E,4E)-5-(4-methoxyphenyl)-1-(pyridin-4-yl)penta-2,4-dien-1-one (MPP), was synthesized and characterized by spectroscopic, crystallographic, computational, and nonlinear optical techniques. Single crystal X-ray diffraction revealed that MPP crystallizes in the non-centrosymmetric monoclinic Pc space group with two crystallographically independent molecules. Hirshfeld surface analysis revealed that H···H and C···H/H···C contacts dominate the crystal packing. The compound showed an absorption maximum at 379 nm with fluorescence emission at 546 nm in solution and 586 nm in the solid state. The Z-scan measurements demonstrated negative nonlinear refraction, and a third-order nonlinear susceptibility of 4.89 × 10^-6 esu. DFT calculations disclosed a HOMO-LUMO energy gap of 3.27 eV. Molecular docking against SARS-CoV-2 main protease (6LU7) showed moderate binding affinity through hydrogen bonding and hydrophobic interactions. These findings suggest the potential of MPP as an organic material for third order nonlinear optical and optoelectronic applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149165242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Application of Indanone-based Turn-on Probe for Mercury/Fluoride Ions in Real Samples.","authors":"Limin Ma, Fei Yang, Haoyang Yao, Zhigang Zhao, Zhichuan Shi","doi":"10.1007/s10895-026-04963-6","DOIUrl":"https://doi.org/10.1007/s10895-026-04963-6","url":null,"abstract":"<p><p>Two novel reactive fluorescent probes, (E)-2-(pyridin-2-ylmethylidene)-5-(phenoxycarbonothioyloxy)-2,3-dihydro-1H-indenone (TP-PMT) synthesized by introducing a phenoxycarbonyl thiosulfinate unit and (E)-2-(pyridin-2-ylmethylidene)-5-((tert-butyldimethylsilyl)oxy)-2,3-dihydro-1H-indenone (TP-TBS) synthesized by introducing a tert-butyldimethylchlorosilane recognition unit, both based on the same (E)-2-(pyridin-2-ylmethylidene)-5-hydroxy-2,3-dihydro-1H-indenone (TP-OH) structure, for highly selective and sensitive detection of mercury ions (Hg²⁺) and fluoride ions (F^-). The TP-PMT demonstrated excellent sensitivity and selectivity for Hg²⁺, showing significant fluorescence enhancement and enabling quantitative detection over the 0-22 μM range, with a low detection limit of 14.7 nM. Similarly, exposure to F^- triggered a distinct fluorescence response in TP-TBS, allowing for quantitative determination in the concentration range of 0-21 μM with a low detection limit of 3.18 nM. Both probes were successfully applied for the reliable determination of their respective target ions in real-world practical samples, demonstrating excellent precision, accuracy, and recovery.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitro-Substituted Azo Dyes as Anti-Prostate Cancer Candidates and Nonlinear Optical Properties: Synthesis and In Silico Studies.","authors":"Mohammed Hafedh Hameed, Adil Muala Dhumad, C A Emshary, Qusay M A Hassan, H A Sultan","doi":"10.1007/s10895-026-04967-2","DOIUrl":"https://doi.org/10.1007/s10895-026-04967-2","url":null,"abstract":"<p><p>Azo dye derivatives are prepared using the diazotation reaction of (o-,m-, and p-) nitroaniline, followed by coupling with 2-naphthaleneol under low-temperature conditions. The prepared azo dyes (1-3) are characterized via FTIR, ¹HNMR, ¹³CNMR, and mass spectroscopy. DFT calculations via B3LYP/6-311 + + G (d,p) provided understanding of the electronic structures, revealing narrow gaps between the HOMO and LUMO at 3.1368 and 3.2713 and 3.2100 eV. Molecular docking studies demonstrated strong affinity of the prepared azo dyes with selected androgen receptor 1E3G. The azo dye substituted with a nitro group showed a low affinity energy at - 8.7, - 7.7, and - 8 kcal/mol and is associated with hydrogen bonds, hydrophobic, and Van der Waals interactions. The nonlinear optical (NLO) properties of azo dyes 1-3 are investigated using the diffraction patterns (DPs) method with 473 nm continuous wave (CW) laser beam. Azo dyes 1 and 2 produced diffraction patterns when the laser beam traversed both separated, whereas no DPs resulted from the third one. The nonlinear refractive index (NL RI) values for azo dyes 1 and 2 are found to be 10.123 × 10^-7 and 7.23 × 10^-7 cm²/W, respectively. These high NLRI, n₂, values make azo dyes 1 and 2 promising candidates for use in optical devices.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Ni (II) based Fluorescent Coordination Architecture for Selective Sensing of Trinitrophenol (TNP) in an Aqueous Medium.","authors":"Meena Devi, Sumit Mittal, Rakesh Kumar, Sadhika Khullar","doi":"10.1007/s10895-026-04966-3","DOIUrl":"https://doi.org/10.1007/s10895-026-04966-3","url":null,"abstract":"<p><p>A Ni (II)-based fluorescent coordination architecture was successfully synthesised using a multifunctional ligand 2-(4-carboxyphenyl)-1 H-benzo[d] imidazole-6-carboxylic acid (L) under solvothermal conditions and characterised using several analytical techniques such as elemental analysis, FTIR spectroscopy, thermogravimetric analysis, and powder X-ray diffraction studies. The synthesised coordination architecture exhibits excellent fluorescent emission in water, attributed to ligand-centred π-π* transitions modulated by metal coordination. Interestingly, this compound demonstrates highly selective and sensitive fluorescence quenching of Trinitrophenol (TNP) among various nitroaromatic explosives, including 4-NT, 2-NT, 2-NP, 3-NP, 4-NP, 1,3-DNB, and 2,4-DNP. A remarkable detection limit of 0.183 ppm was obtained, indicating excellent sensing performance. Density functional theory (DFT) calculations reveal that the sensing mechanism is predominantly ligand-centered and driven by a photo-induced electron transfer (PET) process. The strong electron-accepting character of TNP, together with H-bonding interactions between TNP and 1, promotes efficient electron transfer from the excited ligand to TNP, resulting in significant fluorescence quenching. It also showed rapid response and good stability in aqueous medium, highlighting its potential as an efficient fluorescent probe for trace-level detection of TNP in water.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Label-Free Fluorescent Turn-on Method for the Determination of Guanine Using Levodopa.","authors":"Gasser M Khairy, Esraa I Ali, Eman M Saad, Abbas M Abbas, Saad S M Hassan","doi":"10.1007/s10895-026-04949-4","DOIUrl":"https://doi.org/10.1007/s10895-026-04949-4","url":null,"abstract":"<p><p>A novel simple, direct, selective and rapid fluorometric method is described for the determination of guanine. The method is based on a \"turn-on\" response by the enhancement of the fluorescence of levodopa (L-3,4-dihydroxyphenylalanine (L-DOPA), under optimized conditions. The reaction is subjected to detailed spectroscopic and thermodynamic investigation. Upon addition of guanine, to levodopa, a significant enhancement of the fluorescence intensity is observed, accompanied by a slight bathochromic shift of ~ 10 nm in the emission maximum. This fluorescence response is consistent with an interaction-dependent change in the local environment of L-DOPA. Spectroscopic and association analyses support non-covalent association under the investigated conditions, although the available data do not establish a unique molecular binding geometry or identify the specific functional groups involved. Among the tested nucleobases, guanine produced the most pronounced fluorescence enhancement, demonstrating a preferential analytical response under the optimized conditions. A linear relationship between fluorescence intensity and guanine concentration is obtained over the concentration range of 0-280 µM guanine, with a detection limit of 0.088 µM (0.0133 µg/ml). The proposed method is validated following the standards of ISO/IEC 17025, AOAC, USP, U.S.EPA, and U.S.FDA by measuring data trueness, accuracy, bias, precision, within-day repeatability, between-days reproducibility, standard deviations, robustness, sensitivity, range, durability, stability, and limit of detection. The results reveal good data quality, reliability, and consistency. The method is applied for the determination of guanine in human serum samples using the standard addition (spiking) technique. An average accuracy of (93.3-102.2%) with good precision (mean RSD = 0.67%, n = 3) are obtained. The method provides a simple, label-free, and low-complexity approach for guanine determination in aqueous media, offering a practical alternative to more elaborate sensing platform.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal Complex-Based Fluorescent Probe for Sensitive Determination of Citric Acid in Food Samples via a Ligand-Exchange Reaction: An Innovative and Sustainable Approach.","authors":"Rehab H Elattar, Somaia Hisham, Aya Saad Radwan, Manal A Alossaimi, Adel Ehab Ibrahim, Ahmed Emad F Abbas, Anmar Anwar Khan, Bodour S Rajab, Galal Magdy","doi":"10.1007/s10895-026-04961-8","DOIUrl":"https://doi.org/10.1007/s10895-026-04961-8","url":null,"abstract":"<p><p>A novel, rapid, and eco-friendly spectrofluorimetric method was developed and validated for the sensitive determination of citric acid in food samples using an iron(III)salicylate metal complex as a fluorescent probe. The method may involve a possible ligand-exchange process, in which citric acid preferentially coordinates with iron(III), potentially releasing free salicylate and resulting in a marked increase in fluorescence intensity at 409 nm upon excitation at 295 nm. Experimental parameters, including reagent concentration, pH, solvent type, and reaction time, were systematically optimized to maximize sensitivity. The method was validated according to ICH guidelines, showing excellent linearity over 0.2-4.0 µg/mL (r = 0.9999) and low detection and quantitation limits of 0.028 and 0.086 µg/mL, respectively. High accuracy, precision, robustness, and selectivity were achieved, with no significant interference from common food constituents. Practical applicability was demonstrated through successful determination of citric acid in juice, cola, and ice cream using the standard addition technique. Sustainability assessment using ComplexGAPI, AGSA, and MA tools indicated a favorable environmental profile, as it employs only distilled water, minimizes hazardous organic solvents, avoids heating and complex sample preparation, making it suitable for routine food analysis.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trivalent Terbium Incorporated into Kanemite and Its Photoluminescent Property.","authors":"Yu Ruan, Yu Zhong, Wanyun Feng, Xiaoqiang Shang, Yufeng Chen","doi":"10.1007/s10895-026-04965-4","DOIUrl":"https://doi.org/10.1007/s10895-026-04965-4","url":null,"abstract":"<p><p>Trivalent terbium (Tb³⁺) incorporated into Na-kanemite (Na-Ka) was firstly reported. Various characterizations, including X-ray diffraction (XRD), Scanning electron microscope equipped with energy dispersive spectroscopy (SEM/EDS), Inductively coupled plasma-mass spectrometry (ICP-MS), thermogravimetry (TG), X-ray photoelectron spectra (XPS), and Photoluminescent (PL) spectra were employed to investigate the structure, composition, morphology, and photoluminescent property of samples. XRD result indicated that the Na-Ka was an orthorhombic structure with lattice parameters of a = 7.279(3), b = 20.456(6), and c = 4.942(2)Å. After ion exchange, the product Tb-Ka showed no diffraction peaks except two weak diffraction peaks. SEM images suggested that the Tb-Ka was alike to Na-Ka in the morphology. EDS and XPS results confirmed that the Na⁺ ions in Na-Ka were almost fully replaced by Tb³⁺ ions after ion exchange. Moreover, the Tb element was uniformly distributed throughout the Tb-Ka. Photoluminescence revealed that the Tb-Ka exhibited strong emissions attributed to ⁵D₄→⁷F_J (J = 3, 4, 5, 6) transitions, and the strongest emission was at 542 nm (⁵D₄→⁷F₅). After the Tb-Ka was thermally treated at 200 ℃, 400 ℃, 600 ℃, or 800 ℃ for 2 h, the photoluminescent intensity greatly decreased. The PL decay behavior at 542 nm for both Tb-Ka and its thermally treated samples are well fitted with bi-exponential decay model. The average lifetime of 542 nm for Tb-Ka (0.990ms) was longer than those of thermally treated samples (0.709 ~ 0.767 ms). These results implied the layered kanemite was favorable for the Tb³⁺ luminescence.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrinsic Fluorescence as a Probe of Structural Dynamics and Interactions of Human Adenovirus with Surfactants and Liposomes.","authors":"Nicole Sayuri Miranda Okayama, Menira Souza, Cássia Alessandra Marquezin","doi":"10.1007/s10895-026-04962-7","DOIUrl":"https://doi.org/10.1007/s10895-026-04962-7","url":null,"abstract":"<p><p>In this work, the intrinsic fluorescence of Human Adenovirus serotype 2 (HAdV-2), arising from tryptophan (Trp) and tyrosine (Tyr) residues present in viral proteins, was used to monitor structural changes in the three-dimensional organization of the viral particle as a function of the inactivation method employed (UV irradiation or paraformaldehyde (PFA) treatment), temperature, and the presence of negatively and positively charged surfactants. Steady-state fluorescence anisotropy was used to investigate the interaction between HAdV-2 and zwitterionic or negatively charged liposomes by monitoring both the extrinsic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) inserted into the lipid bilayer and the intrinsic anisotropy of Trp residues from viral proteins. Tyr fluorescence emission proved to be more sensitive to temperature variations, whereas Trp emission was more strongly affected in samples inactivated with PFA. In the presence of surfactants, stronger interactions were observed with negatively charged micelles, resulting in enhanced Tyr fluorescence and quenched Trp fluorescence. The most pronounced effect was observed for the interaction between HAdV-2 and negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol), sodium salt (DPPG) liposomes. In the presence of viral particles, the phase transition temperature (T_m) of DPPG liposomes increased by approximately 11 °C, while no detectable structural changes were observed in the three-dimensional organization of the viral particle itself. Overall, these results highlight intrinsic fluorescence as a valuable approach for probing the structural organization of HAdV-2 under different environmental and interfacial conditions.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the Conjugation of Cyclotriphosphazene Covalent Organic Frameworks to Improve the Fluorescence Detection Performance of Hydrazine.","authors":"Qian Zheng, Liwen Chen, Jinmin Wang, Yong Qin","doi":"10.1007/s10895-026-04964-5","DOIUrl":"https://doi.org/10.1007/s10895-026-04964-5","url":null,"abstract":"<p><p>Hydrazine (N₂H₄) is widely employed in industrial processes; however, its high toxicity poses significant environmental and occupational health risks due to potential leakage or unintended exposure. Consequently, the development of a simple, sensitive and selective method for N₂H₄ detection in environmental matrices is essential. In this work, covalent organic framework (PTCOF) was synthesized via condensation of hexachlorocyclotriphosphazene (HCCP) and 2,4,6-tris(4-aminophenyl)-1,3,5-triazine (TAPT). Leveraging the high reactivity of the P-Cl bonds in PTCOF, aniline was site-selectively grafted onto the framework to yield PTCOF-AN. This functionalization strategically optimized the intrinsic donor-π-acceptor (D-π-A) architecture and enhanced intramolecular charge transfer (ICT) efficiency within PTCOF-AN. PTCOF-AN exhibited fluorescence \"turn-on\" response toward N₂H₄, with low limit of detection (LOD) of 153 nM, representing improvements in both sensitivity and selectivity over PTCOF. The fluorescence enhancement is attributed to the reducing capability of N₂H₄ and the inhibition of ICT process in PTCOF-AN. This work demonstrates a rational design strategy for tailoring the photophysical properties of functionalized COF, offering a promising avenue for N₂H₄ monitoring.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}