Journal of Fluorescence最新文献_第2页

A Novel Benzoxazole-Functionalized Conjugated Mesoporous Polymer for Highly Sensitive and Selective Detection of Fe²⁺ and Fe³. 一种新型苯并恶唑功能化共轭介孔聚合物用于高灵敏度和选择性检测Fe2+和Fe3。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-23 DOI: 10.1007/s10895-025-04377-w

Huaiyi Zhang, Guang Wang

{"title":"A Novel Benzoxazole-Functionalized Conjugated Mesoporous Polymer for Highly Sensitive and Selective Detection of Fe2+ and Fe3.","authors":"Huaiyi Zhang, Guang Wang","doi":"10.1007/s10895-025-04377-w","DOIUrl":"https://doi.org/10.1007/s10895-025-04377-w","url":null,"abstract":"Conjugated micro/mesoporous polymers (CMPs) have emerged as versatile platforms for designing advanced functional materials. In this study, a novel benzoxazole-functionalized CMP, TFPA-DBD, was rationally synthesized, featuring a spherical morphology, well-defined mesoporous structure and robust thermal/chemical stability. When dispersed in DMF, TFPA-DBD exhibits a selective \"turn-off\" fluorescence response toward Fe2+ and Fe3+ ions, achieving low detection limits (LOD) of 0.56 µM and 0.72 µM, respectively. Importantly, TFPA-DBD demonstrates rapid response, high selectivity and strong anti-interference capability during sensing process. The fluorescence quenching mechanism originates from synergistic effects between Fe2+/Fe3+ ions and the nitrogen/oxygen sites within the benzoxazole units, and combines with absorption competition quenching (ACQ) sensing mechanism. Additionally, TFPA-DBD enables the straightforward visual identification of Fe2+ and Fe3+ ions using test paper mode, detectable by the naked eye. Practical applicability was validated through reliable recovery performance in real water samples, highlighting its potential for environmental monitoring applications. This work expands the library of functional CMPs and underscores their utility in developing high-performance sensing systems for transition metal ion detection.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Fluorescent Synthetic Retinoids as Potential RAR Agonists with Anticancer, Molecular Docking and ADME Assessments. 新型荧光合成类维生素a作为潜在的抗肿瘤、分子对接和ADME评估的RAR激动剂。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-23 DOI: 10.1007/s10895-025-04343-6

Esraa Ibrahim, Yara E Mansour, Sameh Soror, Hesham Haffez

{"title":"New Fluorescent Synthetic Retinoids as Potential RAR Agonists with Anticancer, Molecular Docking and ADME Assessments.","authors":"Esraa Ibrahim, Yara E Mansour, Sameh Soror, Hesham Haffez","doi":"10.1007/s10895-025-04343-6","DOIUrl":"https://doi.org/10.1007/s10895-025-04343-6","url":null,"abstract":"Natural and synthetic retinoids are derivatives of vitamin A that mediate different transcriptional activities. Synthesis of fluorescent probes can be a useful tool in cellular imaging and understanding different regulatory signaling pathways. In this study, new fluorescent synthetic retinoid analogues (3a, 3b, 4a, and 4b) were prepared and biologically screened against six different cancer and normal cell lines for assessment of their anticancer and fluorescent activities. A combination of biological assays, such as MTT, flow cytometry, gene and protein expression analysis, DNA fragmentation, and ATPase activity assays, as well as in-silico and ADME studies, was performed. All new compounds showed anti-proliferative activity (2.09-132.70 µM) against different cancer cell types with minimal cytotoxicity (SI > 1), and the Caco-2 cancer cell line was selected for further in vitro investigation. All compounds showed cell cycle arrest at the SubG0-G1 phase with induction of early apoptosis and necrosis. Compound 4b showed a significant apoptotic effect by overexpression of Caspase-3 and Cyt-c genes, followed by compound 3a. All compounds showed anti-inflammatory activity by significantly down-regulating the IL-10 pro-inflammatory marker, while compounds 3b, 4a, and 4b specifically down-regulated IL-6. Retinoic acid receptors (RARs) were suggested as molecular targets confirmed by their overexpression of both gene and protein levels with molecular docking and molecular dynamic simulation studies. All fluorescent compounds showed intracellular fluorescent emission spectra with intracellular lipophilic properties. The new fluorescent synthetic retinoids showed dual activities as anticancer agents with fluorescence properties and can be used as useful probes in a variety of cellular imaging investigations.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Cu(II)-Based Functional MOFs with Fluorescent Sensing Capability toward Fe³⁺ and CrO₄²⁻ Ions. 对Fe3⁺和CrO₄2⁻具有荧光传感能力的双Cu（II）基功能mof。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-22 DOI: 10.1007/s10895-025-04369-w

Yanjun Chen, Haiwen Zhang, Yongshan Wang

引用次数: 0

Systematically Investigating CRISPR/Cas12a Fluorescent Biosensor for Sensitive and Specific Single Nucleotide Variants Detection. 系统研究CRISPR/Cas12a荧光生物传感器用于敏感和特异性单核苷酸变异检测。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-22 DOI: 10.1007/s10895-025-04360-5

Zhenlin Shang, Sitong Liu, Dongxu Liu, Yuer Wang, Xiaojing Pei, Shujing Li, Yifan He, Yigang Tong

{"title":"Systematically Investigating CRISPR/Cas12a Fluorescent Biosensor for Sensitive and Specific Single Nucleotide Variants Detection.","authors":"Zhenlin Shang, Sitong Liu, Dongxu Liu, Yuer Wang, Xiaojing Pei, Shujing Li, Yifan He, Yigang Tong","doi":"10.1007/s10895-025-04360-5","DOIUrl":"https://doi.org/10.1007/s10895-025-04360-5","url":null,"abstract":"Precise identification and detection of single nucleotide variation (SNV) concomitant with excess wild-type DNA is greatly needed for invasive disease diagnosis, pathogens detection and early prediction of drug responsiveness. Many variants of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), notably the D614G and N501Y mutations, have been shown to significantly increase the infectivity of pandemics. We herein investigated CRISPR/Cas12a integrated three types fluorescent reporters and two crRNAs for SNV detection by taking D614G and N501Y variants of SARS-CoV-2 as model examples. We systematically screened all possible base substitutions from positions 0 to 19 and identified the middle position of crRNA could efficiently increase the specificity from both theoretical and experimental standpoints. With selected mutation location of crRNA, we then investigated the specificity of ssDNA, dsDNA and molecular beacon (MB) fluorescent reporters and proved the MB reporters can efficiently increase the discriminatory factors. Furthermore, we designed an additional mutation site on crRNA to increase the specificity. For user convenience, we engineered the lateral flow strips to present the results visualized with the naked eyes. Results of specific variants from Omicron proved the feasibility of clinical applications. These findings indicated that the proposed method is a powerful tool for monitoring the key mutations in pathogens and allows for modifications to incorporate newer upcoming variants.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Sensing Mechanism of an ESIPT-Based Ratiometric Fluorescent Probe for the Detection of Phosgene: A Theoretical Study. 基于esipt的比例荧光探针光气检测传感机制的理论研究。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-22 DOI: 10.1007/s10895-025-04376-x

Qingqing Zhang, Shun Li, Xiaole Qiu, Chuan-Lu Yang

{"title":"Unraveling the Sensing Mechanism of an ESIPT-Based Ratiometric Fluorescent Probe for the Detection of Phosgene: A Theoretical Study.","authors":"Qingqing Zhang, Shun Li, Xiaole Qiu, Chuan-Lu Yang","doi":"10.1007/s10895-025-04376-x","DOIUrl":"https://doi.org/10.1007/s10895-025-04376-x","url":null,"abstract":"Excited state intramolecular proton transfer (ESIPT) process of a novel fluorescence probe 1 and its sensing mechanism for phosgene have been studied theoretically. The optimized geometric configurations and infrared spectroscopy analysis of probe 1 indicate that the intramolecular hydrogen bond (N1-H1···N2) is strengthened upon excitation. Potential energy curves confirm that the energy barrier of probe 1 is smaller in the S1 state (6.21 kcal/mol) than that in the S0 state (15.47 kcal/mol), which promotes the occurrence of the ESIPT process. Theoretical calculations show that the absorption and fluorescence spectra of product are both red-shifted (91 and 77 nm, respectively) compared to the probe 1 due to the obvious charge transfer extent. The electron density difference indicates that the charge transfer distance of product (1.83 Å) is larger than that of probe 1 (1.49 Å), which results in the red-shift of emission of product compared to that of probe 1. Therefore, probe 1 can detect phosgene through the fluorescence variation induced by the large extent of charge transition. This work not only provides a theoretical foundation for designing ESIPT-based fluorescent sensors but also highlights their potential in real-time monitoring of toxic gases.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Binuclear Zn(II) Complex Giving A Large Fluorescent Response Upon Binding PPi Anion. 一种双核锌（II）配合物结合PPi阴离子后产生大的荧光响应。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-21 DOI: 10.1007/s10895-025-04326-7

Joanne Li, Yonghao Li, Yi Pang

引用次数: 0

New Colorimetric Chemosensor for Rapid Naked-Eye Detection of Cu²⁺ and Its Application in Gas-Generating Agents. 新型裸眼快速检测Cu2+的比色化学传感器及其在生气剂中的应用。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-21 DOI: 10.1007/s10895-025-04368-x

Xiaohong Cheng, Jun You, Shaoyong Meng, Wenjie Shen, Tao Du, Wenbin Fu, Yunqiang Luo, Song Wang

引用次数: 0

Quinoline-Thiourea Conjugate: A Dual-Modal Fluorescent Chemosensor for Selective Detection of Mercury and Cyanide Ions in Seafood, Soil, and Biological Systems. 喹啉-硫脲缀合物：一种用于海鲜、土壤和生物系统中汞和氰化物离子选择性检测的双模态荧光化学传感器。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-21 DOI: 10.1007/s10895-025-04353-4

Narmatha G, T Johny Dathees, Elizabeth Antony, J Prabhu, Abhisha Nancy, Abiram Angamuthu, Abdulrahman I Almansour, Raju Suresh Kumar, Raju Nandhakumar

{"title":"Quinoline-Thiourea Conjugate: A Dual-Modal Fluorescent Chemosensor for Selective Detection of Mercury and Cyanide Ions in Seafood, Soil, and Biological Systems.","authors":"Narmatha G, T Johny Dathees, Elizabeth Antony, J Prabhu, Abhisha Nancy, Abiram Angamuthu, Abdulrahman I Almansour, Raju Suresh Kumar, Raju Nandhakumar","doi":"10.1007/s10895-025-04353-4","DOIUrl":"https://doi.org/10.1007/s10895-025-04353-4","url":null,"abstract":"A quinoline-thiourea conjugate probe, AQS, was developed to selectively detect Hg2+ and CN- ions in a DMSO:H2O (1:1) buffer solution using fluorescence spectroscopy. Probe AQS, upon binding with Hg2+, the photon-induced electron transfer (PET) process is inhibited, assisted by the restriction of the C-N bond rotation resulting in significantly enhanced fluorescence intensity. Conversely, CN- binding to the two NH groups triggers electron transfer from hydrogen to CN-, modulating the PET process and enabling the quenching of fluorescence of the probe AQS. The binding stoichiometry of probe AQS with Hg2+ and CN- was determined to be 1:1 and 1:2, respectively, using Job's plot method. Density functional theory (DFT) calculations yielded energy gaps of 3.792 eV for probe AQS, and 0.758 and 0.711 eV for AQS-Hg2+ and AQS-CN- complexes, respectively. The sensor AQS demonstrated practical applicability in detecting Hg2+ in real-world samples, including water, soil, and seafood, using PVA-coated gloves. The probe AQS successfully sensed mercury ions in E. coli bacterial cells, as visualized through laser confocal scanning microscopy, showcasing its potential for biological applications.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Dual Mode Foolproof Sensor Based on Glutathione Stabilized Copper Nanoclusters for Mercuric Ions and its Application in Fluorescence Sensing of Ascorbic acid. 基于谷胱甘肽稳定铜纳米团簇的汞离子双模式万无一失传感器及其在抗坏血酸荧光检测中的应用。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-21 DOI: 10.1007/s10895-025-04342-7

Joel Jose, Lakshmi Nandhana, Merlin Elsa Varghese, Nebu John

{"title":"A Dual Mode Foolproof Sensor Based on Glutathione Stabilized Copper Nanoclusters for Mercuric Ions and its Application in Fluorescence Sensing of Ascorbic acid.","authors":"Joel Jose, Lakshmi Nandhana, Merlin Elsa Varghese, Nebu John","doi":"10.1007/s10895-025-04342-7","DOIUrl":"https://doi.org/10.1007/s10895-025-04342-7","url":null,"abstract":"Sensors that are handy for the common man are highly demanded. Unfortunately, fluorescence sensors alone cannot satisfy this need. Hence here we introduce a dual mode foolproof sensor based on glutathione stabilized copper nanoclusters (GSH-CuNCs) for sensing mercuric ion (Hg2+), that can combine the features of both worlds, that is the handiness of a colorimetric sensor and the ultra-sensitivity of a fluorescence sensor. On exciting at 300 nm, GSH-CuNCs gave fluorescence emission at 450 nm with a stokes shift of 150 nm and the emission was dependent of excitation wavelength. GSH-CuNCs was characterized using UV-visible spectroscopy, Photoluminescence (PL), Fourier Transform Infrared Spectroscopy (FTIR), Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI TOF), Transmission Electron Microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Time-Correlated Single Photon Counting studies (TCSPC) studies. It was found that the synthesized probe fits great to serve both as a fluorescence and colorimetric probe in sensing the presence of Hg2+. Hg2+ caused the quenching of fluorescence of the probe. Ascorbic acid (AsA) was used to turn on the fluorescence of this quenched system. Both quenched (Turn off) and turned on system were also studied by employing PL, TCSPC, TEM and XPS techniques. Colorimetric studies found the change in colour of the probe from light yellow to dark brown. The limit of detection (LoD) of Hg2+ was found to be 161.6 nM fluorometrically and 1.5 µM colorimetrically. LoD of AsA was found to be 39.17 nM fluorometrically. This work outshines other similar dual mode sensing techniques for Hg2+ in having simple synthesis and sensing strategy with almost similar sensitivity and selectivity. The feasibility of this sensing strategy to be embedded in complex logic devices for multiplex analysis has also been explored.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anionic Surfactant-Triggered Excimer Emission from Dipyrene-Based Amphiphilic Dyes for Wash-Free Cell Membrane Imaging. 阴离子表面活性剂引发的二芘基两亲性染料的准分子发射用于无水洗细胞膜成像。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2025-05-20 DOI: 10.1007/s10895-025-04304-z

Yongling Zheng, Bailin Guo, Enju Wang, Bingxin Lei

引用次数: 0