Journal of Fluorescence最新文献

{"title":"Synthesis, Computational, DFT Calculations, Photophysical and Docking Studies of Novel Fluorescent Pyrimidine-Chlorothymol Hybrid as Potent Antimicrobial Agent.","authors":"S Chandrasekhar, B S Prathibha, S Bindu, M S Raghu, M K Prashanth, K Yogesh Kumar, Fahd Alharethy, Byong-Hun Jeon","doi":"10.1007/s10895-025-04274-2","DOIUrl":"https://doi.org/10.1007/s10895-025-04274-2","url":null,"abstract":"<p><p>Molecular fluorescent probes have found numerous applications in material science and biology. Fluorescently labelled heterocyclic compounds are found usage in light-emitting diodes, high throughput screening, bioanalytical applications, and diagnostics. Hence, this study focuses on the synthesis of a novel functionalized 4-((6-chloro-2-(trifluoromethyl)pyrimidin-4-yl)amino)-2-isopropyl-5-methyl phenol (CTPP) molecule using molecular hybridization approach as an antimicrobial agent and fluorescent probe. The synthesized CTPP molecule was characterized utilizing various spectroscopic techniques. The synthesized CTPP molecule was validated for their antimicrobial studies. With MIC values of 11.8 ± 0.26 and 14.6 ± 0.35 µM against S. aureus and C. albicans strains, respectively, the CTPP molecule significantly surpassed the reference medications streptomycin and fluconazole. The molecular docking results revealed that CTPP molecules has shown favourable binding interactions with good binding site energies against the target 3VO8 and 7PJC proteins. Computational studies were performed using the DFT method with a B3LYP/6-311G basis set used to explore the molecular geometry, global reactive descriptors, MEP, absorption, emission, ELF, LOL, RDG and NLO characteristics. It was discovered that the CTPP molecule's calculated NLO values were superior to reference molecule urea in both the gas and solvent phases. Moreover, the first and second hyperpolarizability values for CTPP molecule in gas and DMSO phase indicate that they could be employed as good nonlinear optical materials. The results of both theoretical and experimental research demonstrate that the CTPP molecule is a promising one that can be applied to NLO-based products in the optoelectronic industry.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive and Facile Detection of Vitamin D Based on Fluorescent Labeled Aptamer Probe and Exonuclease I-Assisted Signal Amplification.","authors":"Xinqiu Xu, Chaofan Jia, Fengjiao Zhang, Hao Li, Weilei Gong, Changqin Wang, Yin Wei","doi":"10.1007/s10895-025-04282-2","DOIUrl":"https://doi.org/10.1007/s10895-025-04282-2","url":null,"abstract":"<p><p>This study presents a rapid, simple aptamer-based fluorescence sensor using fullerenes as a quencher for detecting vitamin D3. Fullerenes, with its π-electron cloud, acts as a powerful electron acceptor, facilitating Förster resonance energy transfer (FRET) from carboxyfluorescein (FAM). The aptamer, labeled with 5'6-FAM, adsorbs onto the fullerenes surface through hydrogen bonding and π-π stacking interactions, leading to fluorescence quenching. Upon vitamin D3 binding, the aptamer forms a hairpin structure that prevents adsorption onto the fullerenes surface, restoring fluorescence. The fullerenes were characterized using UV-Vis, FT-IR, Raman spectroscopy, SEM, TEM, and zeta potential measurements. The sensor's response to varying vitamin D3 concentrations was analyzed with a fluorescence spectrometer, revealing a linear detection range of 0-600 nM and a detection limit of 200 nM, which improved to 50 nM with exonuclease I. The sensor demonstrated a recovery rate of 88.4%-96.3% for vitamin D3 in water samples, confirming its feasibility for practical applications. In addition, our proposed sensor exhibited good repeatability (3.27%) and stability (90%). The innovative use of fullerenes as a fluorescence quencher, along with the aptamer's specific binding to vitamin D3, provides a novel and highly sensitive avenue for vitamin D3 detection.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Impact of Heavy Atom Substitutions (Se, Te) on the CB16-Based Non- Fullerene Acceptor for High-Performance Organic Solar Cells: DFT and TD-DFT Study.","authors":"Walid Taouali, Kamel Alimi","doi":"10.1007/s10895-025-04268-0","DOIUrl":"https://doi.org/10.1007/s10895-025-04268-0","url":null,"abstract":"<p><p>Heavy atom substitution is a promising strategy for enhancing organic solar cells (OSCs) performance. This study explores the optoelectronic impact of such substitution on the recently synthesized non-fullerene acceptor CB16 (C₀). Specifically, two derivatives, C₁ and C₂, were designed, replacing sulfur (S) with two distinct heavy heteroatoms selenium (Se) and tellurium (Te), respectively. Their photophysical and optoelectronic characteristics have been investigated through density functional theory (DFT) and time-dependent DFT simulations using the HSEH1PBE/6-311G(d,p) method. The computational investigations covered a wide range of properties, including electronic structure and bonding characteristics, electronic density and stability, reorganization energy, absorption properties and non-linear optical response. Interestingly, in both C₁ and C₂, the energy difference between the highest occupied and the lowest unoccupied molecular orbitals (HOMO-LUMO) was significantly reduced, decreasing from1.71 eV in C₀ to 1.64 eV in C₁ and 1.54 eV in C₂. Similarly, the simulation results showed several enhanced properties in both derivatives compared to C₀, with C₂ demonstrating markedly greater improvements such as enhanced dipole moment (2.35D), red-shifted absorption indicating enhanced light-harvesting potential, and extended excited state lifetime (4.94 ns) owing it to its low hole reorganization energies (λh ~ 0.173 eV). Analysis of molecular orbitals, transition density matrix, and bonding characteristics suggests that Se and Te substitutions facilitate improved intramolecular charge transfer and enhanced non-linear optical response and provide a higher photovoltaic potential. These findings highlight the effectiveness of heavy atom substitution in tuning optoelectronic properties and the potential of targeted heavy tellurium atom substitution for the development of next-generation high-performance organic solar cells.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DFT and Experimental Investigation of the Optoelectronic Properties of TFB Polymer for Lasing Application.","authors":"Mamduh J Aljaafreh, Boutheina Ben Abdelaziz, N Mustapha, Osamah Aldaghri, Hajo Idriss, K H Ibnaouf","doi":"10.1007/s10895-025-04236-8","DOIUrl":"https://doi.org/10.1007/s10895-025-04236-8","url":null,"abstract":"<p><p>The current work studies the electronic and optical properties of a conjugated polymer Poly(9,9-dioctylfluorene-alt-N-(4-sec-butylphenyl)-diphenylamine) (TFB) after dissolving in tetrahydrofuran (THF) in different concentrations ranging from 2.5 to 0.6 mg/mL. It shows an absorption maxima at 390 nm, however, an increase in concentration (2.5 mg/ml) gives an additional band at 482 nm. This additional band suggests the presence of ground-state aggregation. On the other hand, its fluorescence spectrum at different concentrations ranging from 30 to 1.88 mg/mL showed two distinctive bands around 433 and 451 nm and a tail at 480 nm. The experimental optical bandgaps of TFB are evaluated to be 2.95-2.83 eV depending on its concentration. Its computed electronic structures (n = 1 to 4) are also explored by using the density functional theory (DFT). The observed fluorescence emission of TFB can be represented by the superposition of three modified Franck-Condon progressions. The transition energy (E₀) is red-shifted for all Franck-Condon Fits due to increasing the concentration of TFB. The calculated HOMO-LUMO bandgap and absorption spectra were compared with the experimental result. The amplified spontaneous emission (ASE) characteristics of TFB in THF at and under various pump power excitations were studied. The results show the ASE peaked at 443 nm with an FWHM of approximetely11 nm, and the threshold energy was 2 mJ.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Photophysical Characteristics of Samarium-based Complexes for Optoelectronic and Latent Fingerprint Applications.","authors":"Shagun Goyat, Vaishnavi Lather, Aarti Khatkar, Bhawna Rathee, V B Taxak, S P Khatkar, Pratibha Ahlawat, Poonam Kumari, Vipin Kumar, Rajesh Kumar","doi":"10.1007/s10895-025-04250-w","DOIUrl":"https://doi.org/10.1007/s10895-025-04250-w","url":null,"abstract":"<p><p>In this study, five orange-red color samarium (Sm(III)) complexes were prepared using a solution precipitation method, with 9,10-Difluoro-2,3-dihydro-3-methyl-7-oxo-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid as the primary ligand and nitrogen-donor like 1,10-phenanthroline, 2,2-biquinoline, neocuproine, and 2,2-bipyridyl as secondary ligands. The complexes were characterized using various spectral techniques, elemental analysis CHN, energy-dispersive X-ray spectroscopy (EDX), ultraviolet-visible (UV-vis), Fourier-transform infrared (FTIR), nuclear magnetic resonance (NMR) and thermal analyses by thermogravimetric analysis (TGA), differential thermal analysis (DTA). Photoluminescence spectroscopy revealed bright orange-red emissions due to the ⁴G_5/2 → ⁶H_7/2 transition at 605 nm in solid and 598 nm in solution, with color coordinates confirming their emission in the orange region. Structural evaluations using powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM) confirmed that the complexes exhibit nanoparticle size and crystalline structure, showing their application in forensic science in latent fingerprint detection. Computational studies of highest occupied molecular orbital (HOMO)-lowest unoccupied molecular orbital (LUMO) energy levels, Judd-Ofelt parameters, radiative properties, band gap, and Urbach energy indicate their potential for optoelectronic and semiconductors. The high color purity of 87.70% in solution and 77.50% in solid make them important for laser emission.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-Friendly Synthesis of Nitrogen-Doped Fluorescent Carbon Dots from Citrus Juice for Detection of Fe³⁺ Ions and Anti-Counterfeiting Printing Applications.","authors":"Tianbao Fang, Lei Wu, Shaoyun Huang, Ting Guo, Yafeng Deng","doi":"10.1007/s10895-025-04247-5","DOIUrl":"https://doi.org/10.1007/s10895-025-04247-5","url":null,"abstract":"<p><p>Recently, the use of natural products to synthesize of carbon dots (CDs) has received much attention. Herein, citrus juice and urea were successfully turned into nitrogen-doped carbon dots (N-CDs) via the hydrothermal-carbonization route. The synthesized N-CDs have good fluorescence luminescence performance and high optical stability. In addition, the N-CDs exhibited selective and sensitive fluorescence quenching behavior towards Fe³⁺ ions and the limit of detection (LOD) can reach 1.59 µM. Moreover, environmentally friendly fluorescent inks were prepared with N-CDs as fluorescent material and cellulose (HPC, HEC, HEMC, HPMC) as a binder. These fluorescent inks meet the viscosity (4000-12000 mPa·s) and surface tension (20-60 mN/m) requirements of screen printing. Fluorescent ink still maintains good fluorescence after forming a film on filter paper.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Colorimetric/Fluorescence Dual Detection for Acid Phosphatase Based on Copper Nanoclusters.","authors":"Yuanyuan Cheng, Xiujuan Huang, Yifeng Tu, Qi Ma, Jilin Yan","doi":"10.1007/s10895-025-04261-7","DOIUrl":"https://doi.org/10.1007/s10895-025-04261-7","url":null,"abstract":"<p><p>A simple and effective fluorescent/colorimetric dual detection was established toward acid phosphatase (ACP). Photoluminescent copper nanoclusters with blue emission were synthesized. These nanoclusters were also found with peroxidase-like activity and capable of accelerating the conversion of 3,3',5,5'-tetramethylbenzidine (TMB) into a colored oxidized form (oxTMB). As a strong ligand toward copper, pyrophosphate (PPi) inhibited this catalysis. When ACP was present, it hydrolyzed PPi into non-binding phosphate, and thus the inhibited catalysis was recovered. This brought in an increase in absorbance from oxTMB for a colorimetric signaling. Meanwhile, due to a good overlap of the emission of the CuNCs and absorption of oxTMB, the fluorescence signal was attenuated through inner filter effect (IFE). Both the increase of the absorption and decrease in fluorescence were applied for the quantification of ACP activity. The colorimetric signals provided a linear response toward ACP in the range 0.1-10 U/L, with a detection limit of 0.01 U/L, while the fluorimetry responded within 0.005-10 U/L, with a detection limit of 0.001 U/L. Because the same reagents and steps were applied, the method allowed a facile switching between these two modes, depending the performances required and instrumentations available. The applicability of the detection was validated through analysis of human serum samples.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Binding, Molecular Docking and Antimicrobial Studies of Co(III) Polypyridyl Complexes.","authors":"Shyam Perka, Ravi Kumar Vuradi, Srinivas Gopu, Navaneetha Nambigari, Satyanarayana Sirasani","doi":"10.1007/s10895-025-04222-0","DOIUrl":"https://doi.org/10.1007/s10895-025-04222-0","url":null,"abstract":"<p><p>Three new cobalt(III) polypyridyl complexes [Co(phen)₂(TIPB)]³⁺(1) {TIPB = 5-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)thiophene-2-yl-2-boronic acid}, (phen = 1,10-Phenanthroline), [Co(bpy)₂(TIPB)]³⁺(2) (bpy = 2,2'bipyridyl), [Co(dmb)₂(TIPB)]³⁺(3) (dmb = 4, 4'-dimethyl 2, 2'-bipyridine) have been synthesized and characterized by spectral studies FTIR, UV-vis, ¹H, ¹³C-NMR, Mass Spectroscopy and Elemental analysis. The binding properties of all three complexes towards calf-thymus DNA (CT-DNA) have been investigated using UV-visible, emission spectroscopy and viscosity measurements, indicating that all the complexes bind to CT-DNA predominantly through intercalation, but with different affinities. The binding strength of these complexes were 1 > 2 > 3. These complexes cleaved the pBR322 DNA into different forms when irradiated under UV light. The binding studies of Co(III) polypyridyl complexes with ds-DNA were carried out by molecular modeling (MM) studies to identify the binding interactions. The molecular mechanics studies were performed on metal complexes to obtain stable 3D conformers. Double-stranded DNA was docked to metal complexes' energy-minimized three-dimensional structures. Through intercalation, the aromatic ligand TIPB makes it easier for the metal complex to bind to DNA. The necessary characteristics for metal complexes to be regarded as DNA intercalators are their substantial interactions with DNA. The available experimental data provides strong support for the molecular docking data. The binding selectivity and strength of the metal complex with DNA show that the preferred binding mode involves guanine rich site. It reveals that complexes will bind in between base pairs of DNA and gives information about the binding residues and strength. The antimicrobial studies of these complexes give considerable activity with different bacteria like Staphylococcus aureus, and Bacillus subtilis.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red Random Laser Based on Thermocavitation : Synthesis and Characterization.","authors":"Jassim Mohammed Jassim, Majid F Haddawi, M J Mohammed, S M Hamidi","doi":"10.1007/s10895-025-04225-x","DOIUrl":"https://doi.org/10.1007/s10895-025-04225-x","url":null,"abstract":"<p><p>In this work, we studied thermal blooming phenomena using a CW laser with a wavelength of 405 nm and an optical power of 100 mW focused on a quartz cuvette containing dye solution with different types of nanoparticles to generate thermocavitation. We observed that self-phase modulation (SPM) typical far-field loops suddenly shifted into a completely different diffraction pattern when the dye solution was heated beyond the self-phase modulation (SPM) using a focused laser beam. After SPM, this process, known as thermal blooming (TB), can be considered the next phase. In addition, we observed the effect of (Ag NWs, Fe₂O₃, and AgNWs: Fe₂O₃) NPs on the speed of bubble formation and their number. also observed random laser actions in both the presence and absence of thermal cavitation. The presence of a thermal cavity was found to have a significant impact on the pump threshold, spectral linewidth, and intensity of emission spectra.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonlinear Optical Features of Phenol Red Dye in Different Solvent Media Using Z-scan and Density Functional Theory.","authors":"B Anusha, A G Bharathi Dileepan, Rohith Ramasamy, Rajadurai Vijay Solomon, Natarajan Arumugam, Abdulrahman I Almansour, Kasireddy Sudarshan, S Jeyaram","doi":"10.1007/s10895-025-04224-y","DOIUrl":"https://doi.org/10.1007/s10895-025-04224-y","url":null,"abstract":"<p><p>This study extensively discusses the nonlinear optical properties of phenol red dye, which is commonly used as an indicator dissolved in various solvents. The fluorescence analysis indicates that the phenol red dye exhibits excitation in the red region of the spectrum. Z-scan tool is employed to evaluate the third-order nonlinear optical parameters of phenol red dye in polar liquids. Z-scan study accomplished by using a diode laser with 405 nm wavelength and an output intensity of 741 W/cm². Both closed and open aperture Z-scan techniques yield significant values for the nonlinear refractive index and absorption coefficient of phenol red dye. The order of nonlinear refractive index and absorption coefficient of phenol red dye are found to be of 10⁻⁷ cm²/W and 10⁻² cm/W, respectively. A large second-order hyperpolarizability of phenol red dye is observed, with a value on the order of 10⁻³¹ esu. The density functional theory studies at the B3LYP/6-311 +  + G(d,p) level provide the linear polarizability (α), first-order hyperpolarizability (β), and second-order hyperpolarizability (γ) values in ethanol, methanol, DMF, and DMSO, supported by experimental results. Multi-parameter scale is applied to assess the impact of solvents on phenol red dye and implies that both specific and non-specific interactions contribute to the resulting nonlinearity in phenol red dye.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}