Journal of Fluorescence最新文献

{"title":"Fabrication of Luminescent Microtiterplate Using Terbium Complex for Phenol Screening in Seawater Samples.","authors":"Rasha M Kamel","doi":"10.1007/s10895-024-03639-3","DOIUrl":"10.1007/s10895-024-03639-3","url":null,"abstract":"<p><p>Tb(III)-2-aminoterphthalate complex Tb₂-(ATPh)₃ was synthesized and characterized using FT-IR, thermal analysis and elemental analysis. Tb₂(ATPh)₃ microtiter plate was fabricated through embedding Tb(III) complex in polyvinyl chloride membrane and used for environmental determination of phenol in sea water samples. The calculated detection (D_L), quantification (Q_L) limits, and binding constant (K_D) were 00.63 µmol L^- 1, 2.10 µmol L^- 1 and 1.32 × 10⁴ mol^- 1 L, respectively. The fabricated microtiter plates exhibited high selectivity towards phenol over other hydrocarbon compounds. Furthermore, AGREE metric tool was used to assess the method's green nature as well as its practicability and applicability. These merit outcomes provide that the new method for phenol detection was environmentally benign and safe to humans. The prepared Tb₂(ATPh)₃ MTP was validated through using gas chromatography for monitoring phenol in Suez Bay water accurately with high precision. The obtained results encouraged using Tb₂(ATPh)₃ MTP for efficient, fast, selective, and direct screening of phenol in real samples.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1833-1839"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140059503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Detection of Prolactin and Growth Hormone Using a Dual-label Time-resolved Fluorescence Immunoassay.","authors":"Shuhai Zhong, Huankun Liang, Fenglan Peng, Yanhong Lu, Tiancai Liu, Uladzimir Kulchytski, Wenqi Dong","doi":"10.1007/s10895-023-03395-w","DOIUrl":"10.1007/s10895-023-03395-w","url":null,"abstract":"<p><p>Prolactin (PRL) and growth hormone (GH) are two important hormones secreted by the pituitary gland, and their abnormal levels are often related to disease status. This study aimed to establish a new dual-label time-resolved fluorescence immunoassay (TRFIA) to quantitatively measure PRL and GH levels in serum. A sandwich TRFIA was optimized and established: anti-PRL/GH antibodies immobilized on 96-well plates captured PRL/GH and then banded together with anti-PRL/GH paired antibodies labeled with europium(III) (Eu³⁺)/samarium(III) (Sm³⁺) chelates. Finally, a time-resolved analyzer measured the Eu³⁺/Sm³⁺ fluorescence values. Clinical serum samples were used to evaluate the detection performance of this method. The sensitivities of this dual-label TRFIA were 0.35 ng/mL and 0.45 ng/mL, respectively, and the detection range was between 0.1 and 1000 ng/mL. All the cross-reactivities were lower than 1.07%. The intra-assay and interassay coefficients of variation were 2.18-7.85% and 2.25-7.30%, respectively. Compared with the registered TRFIA kits, a high Pearson coefficient (r = 0.9626 and 0.9675) was observed. This dual-label TRFIA has high sensitivity, accuracy and specificity with good clinical detection performance, representing a suitable alternative to existing methods for determining PRL and GH levels, and is expected to be used in the clinic in the future.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1417-1422"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adulteration Detection and Quantification in Olive Oil Using Excitation-Emission Matrix Fluorescence Spectroscopy and Chemometrics.","authors":"Zhang Lujun, Cai Nuo, Huang Xiaodong, Fan Xinmin, Gao Juanjuan, Gao Jin, Li Sensen, Wang Yan, Wang Chunyan","doi":"10.1007/s10895-024-03613-z","DOIUrl":"10.1007/s10895-024-03613-z","url":null,"abstract":"<p><p>This research investigates the use of excitation-emission matrix fluorescence (EEMF) in conjunction with chemometric models to rapidly identify and quantify adulteration in olive oil, a critical concern where sample availability is limited. Adulteration is simulated by blending soybean, peanut, and linseed oils into olive oil, creating diverse adulterated samples. Principal component analysis (PCA) was applied to the EEMF spectral data as an initial exploratory measure to cluster and differentiate adulterated samples. Spatial clustering enabled vivid visualization of the variations and trends in the spectra. The novel application of parallel factor analysis (PARAFAC) for data decomposition in this paper focuses on unraveling correlations between the decomposed components and the actual adulterated components, which offers a novel perspective for accurately quantifying adulteration levels. Additionally, a comparative analysis was conducted between the PCA and PARAFAC methodologies. Our study not only unveils a new avenue for the quantitative analysis of adulterants in olive oil through spectral detection but also highlights the potential for applying these insights in practical, real-world scenarios, thereby enhancing detection capabilities for various edible oil samples. This promises to improve the detection of adulteration across a range of edible oil samples, offering significant contributions to food safety and quality assurance.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1819-1832"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140059501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Chromogenic Probe for Detection of Biologically Important Anions and its Implications for Designing Molecular Logic Gates.","authors":"Madan Rajbanshi, Manas Mahato, Arpita Maiti, Sabbir Ahamed, Pallobi Sarkar, Sudhir Kumar Das","doi":"10.1007/s10895-024-03629-5","DOIUrl":"10.1007/s10895-024-03629-5","url":null,"abstract":"<p><p>Detection of fluoride (F^-), acetate (AcO^-), and cyanide (CN^-) anions is vital from the biological and environmental aspects. In the present contributions, we have introduced a simple Salen-type chromogenic sensor, BEN, to detect these biologically important anions. Changes in UV-visible absorption spectra and color of BEN solution from very pale yellow to pink color are similar for each of these anions and found to be reversible only in the case of F^- ions in attendance of HSO₄^- ions. The estimated limit of detection of BEN solution for detecting F^-, AcO^-, and CN^- anions is found to be below the micromolar (μM) concentration level. Our fabricated handy paper test kit is suitable for qualitatively naked-eye detection of the anions. An immediate quantitative estimation of these important anions is possible using our BEN employing a smartphone, avoiding any costly experimental setup.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1749-1760"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140039551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective Detection of Cu(II) Ions Based on Carbon Dots@Exfoliated Layered Double Hydroxides Composites Fluorescence Probe.","authors":"Quliang Lu, Yixian Mei, Yuting Wu, Houjun Lin, YanLi Li","doi":"10.1007/s10895-024-03597-w","DOIUrl":"10.1007/s10895-024-03597-w","url":null,"abstract":"<p><p>A series of carbon dots@exfoliated layered double hydroxides (CDs@LDH) composites were hydrothermally fabricated by Mg/Al LDH and formamide. The results of FTIR, UV-vis, and XPS spectra in company with HRTEM images showed that crystalline nano CDs formed on the single layer of LDH by Mg-C bond. With the increase of solvothermal reaction time from 2 to 6 h, the band gap and the binding energy of aminic and graphitic N species of CDs@LDH composites decreased, whereas the crystallinity increased. The fluorescence peaks of CDs@LDH composites could be deconvoluted into short-wavelength (416 nm) and large-wavelength (443 nm) components by Gaussian function, and the fluorescence intensities of both components enhanced with the extension of the solvothermal reaction time. The simultaneous enhancements of fluorescence lifetime and quantum yield resulted from the relatively high electron density in graphitic nitrogen of CDs@LDH, whereas the reduction of nonradiative rate was due to the high crystallinity in the carbon core of CDs@LDH. A strong exciton-lattice interaction also has been validated based on the excitation and emission spectra of CDs@LDH, so the fluorescence emission of CDs@LDH composite was heavily related to its crystalline carbon core and nitrogen-containing groups. CDs@LDH with high nitrogen-containing exhibited a superior detection property for Cu²⁺ ion sensing with the linear range of 26.90 ~ 192.20 μM and a limit of detection of 0.1957 μM. The photo-induced electron transfer (PET) process dominated the fluorescence quenching of CDs@LDH by Cu²⁺ ion since the fluorescence lifetime decreased with the increase of Cu²⁺ ion concentration.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1441-1456"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139735406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-Based Quantitative Measurement of Cu²⁺: Fluorescent Turn-on Chemosensor via Radical Cation Formation.","authors":"Dongkyun Gil, Boeun Choi, Cheal Kim","doi":"10.1007/s10895-024-03635-7","DOIUrl":"10.1007/s10895-024-03635-7","url":null,"abstract":"<p><p>We report a unique radical cation formation-based fluorescent chemosensor (E)-N'-(4-(diphenylamino)benzylidene)thiophene-2-carbohydrazide (DBTC) that quantitatively determines Cu²⁺ based on the RGB model using a smartphone. DBTC exhibited a weak turquoise fluorescence due to fluorescence suppression by amide isomerization. When Cu²⁺ was added into DBTC, it showed strong light blue fluorescence with a high quantum yield ([Formula: see text] = 0.470). The detection limit of Cu²⁺ was determined to be 0.40 µM at the concentration range of 0-7.5 µM. In addition, the detection mechanism of DBTC for Cu²⁺ was demonstrated to be an oxidative cyclization reaction through ¹H NMR titration, ESI-MS analysis, and DFT calculation. Remarkably, DBTC could be applied to the quantitative measurement of Cu²⁺ using a smartphone and RGB analysis. The detection limit was calculated to be 0.05 µM, which is the lowest detection limit among chemosensors that could detect Cu²⁺ through smartphone-based fluorescence measurements. Additionally, spike and recovery experiments conducted with different concentrations of Cu²⁺ showed good recovery values. DBTC exhibited its potential as a chemosensor for determining Cu²⁺ through the application of a smartphone-based platform capable of real-time monitoring.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1707-1717"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140028155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chalcone-based Turn-Off Chemosensor for Selective and Susceptible Detection of Fe²⁺ Ions: Spectroscopic and DFT Investigations.","authors":"Bhavya Nelligere Revanna, Vinuta Kamat, Ananda Swamynayaka, Keshav Kumar Harish, Keerthikumara Venkatesha, Mahendra Madegowda, Boja Poojary, Sanjay S Majani, Shiva Prasad Kollur","doi":"10.1007/s10895-024-03646-4","DOIUrl":"10.1007/s10895-024-03646-4","url":null,"abstract":"<p><p>Herein, in this report we are introducing newly synthesized chalcone derivative, \"(E)-1-phenyl-3-(4-((5-(((Z)-thiophen-2-ylmethylene)amino)-1,3,4-thiadiazol-2-yl)thio)phenyl)prop-2-en-1-one\" (5), as a chemosensor to detect Fe²⁺ metal ions in HEPES buffer solution of pH 7.5. Spectroscopic techniques were used to confirm the synthesized sensor. To determine the chemical reactivity and molecular stability of the probe, a frontier molecular orbitals investigation was carried out. A molecular electrostatic potential map was investigated to know the binding site of 5 for metal ion coordination. The theoretical absorption and fluorescence emission properties were estimated and correlated with the experimental observations. The sensor showed excellent selectivity for Fe²⁺ compared to all other studied metal ions. The fluorescence binding studies were carried out by adding different amounts of Fe²⁺ ions for a fixed concentration of probe 5. The inclusion of Fe²⁺ ions resulted in a decrease in fluorescence intensity with a bathochromic shift of emission wavelength of 5 due to the 5-Fe²⁺ complexation. The binding affinity value for the probe was found to be 576.2 M^-1 with the help of the Stern-Volmer plot. The Job's plot and mass spectra supported the 2:1 (5: Fe²⁺) stoichiometry of complex formation. The detection limit and limit of quantification of 5 for Fe²⁺ were calculated to be 4.79 × 10^-5 M and 14.54 × 10^-5 M. Further, in addition to this, the photophysical parameters such as fluorescence lifetime of 5 and 5-Fe²⁺ complex measured to be 0.1439 and 0.1574 ns. The quantum yield of 5 and 5-Fe²⁺ was found to be 0.0398 and 0.0376. All these experimental findings revealed that probe 5 has excellent selectivity and sensitivity for Fe²⁺ ions.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1781-1795"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140059502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidized Bisindolyl-Based Amphiphilic Probe for Dual Mode Analysis of Heavy Metal Pollutants in Aqueous Medium.","authors":"Animesh Pal, Nilanjan Dey","doi":"10.1007/s10895-023-03393-y","DOIUrl":"10.1007/s10895-023-03393-y","url":null,"abstract":"<p><p>The oxidized bisindolyl-based amphiphilic, chromogenic probe has been synthesized that can form nanoscopic aggregates in the aqueous medium. Along with solvent polarity and pH of the medium, it was observed that the addition of heavy metal pollutants, like Hg²⁺ can cause significant alteration in the charge transfer state. This resulted in the immediate change in the solution color from yellow to orange. Additionally, we could excite either the monomer species or the aggregates of the probe by choosing the proper excitation wavelength. Upon exciting at 390 nm, the compound exhibited a broad fluorescence spectrum with maxima at 450 nm, presumably due to twisted state charge transfer. On the contrary, the aggregated species (λex = 465 nm) displayed a comparatively weaker fluorescence band centered at 565 nm. Interestingly, the fluorescence intensity at the 450 nm band experience fluorescence quenching in the presence of Hg²⁺ ion, while the aggregate emission band remained unaffected. Finally, the present system was utilized for detection of mercury ions in natural water samples.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1335-1344"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Influence of annealing on the structural, morphological, photoluminescence and visible absorption properties of Mg doped CuO micro grains.","authors":"P Santhosh Kumar, J Johnson, C S Biju","doi":"10.1007/s10895-023-03468-w","DOIUrl":"10.1007/s10895-023-03468-w","url":null,"abstract":"","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1855"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Enhanced Fluorescent Probe for Simultaneous Detection and Discrimination of Hydrogen Bisulfite Anions and Glutathione.","authors":"Yu Xie, Xiaoci Lv, Zhiwei Li, Yanbo Li, Heping Li","doi":"10.1007/s10895-024-03654-4","DOIUrl":"10.1007/s10895-024-03654-4","url":null,"abstract":"<p><p>Bisulfite (HSO₃^-) and biological thiols molecules, such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), play important roles in organisms. Developing a fluorescent probe that can simultaneously detect and distinguish HSO₃^- and biological thiols is of great significance. In this study, ethyl(2E,4Z)-5-chloro-2-cyano-5-(7-(diethylamino)-2-oxo-2 H-chromen-3-yl)penta-2,4-dienoate (CCO) as a novel enhanced fluorescence probe was synthesized by integrating coumarin derivatives and ethyl cyanoacetate, which can simultaneous detection and discrimination of hydrogen bisulfite anions and glutathione. The sensing mechanism was elucidated through spectral analysis and some control experiments. In weakly alkaline environments, the probe not only has good selectivity for HSO₃^- and GSH, but also has a lower detection limits of 0.0179 µM and 0.2034 µM. The probe exhibited fuorescent turn-on for distinguishing with 296 and 28 fold the fluorescent intensity increase at 486 and 505 nm, respectively, through diferent excitation wavelengths. This provides a new method for simultaneous detection and discrimination of HSO₃^- and biological thiol cell levels and further applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"1797-1805"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140059499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}