Journal of Fluorescence最新文献

{"title":"Selective and Sensitive Fluorescent Detection of Cyanide Ions and their Estimation in a Highly Alkaline Medium.","authors":"P Ponlakshmi, V Dharaniprabha, Kuppanagounder P Elango","doi":"10.1007/s10895-025-04513-6","DOIUrl":"https://doi.org/10.1007/s10895-025-04513-6","url":null,"abstract":"<p><p>Accurate detection of cyanide ions in food and water is indispensable to avert detrimental effects on human health and minimize environmental defilement. In response to this pressing need, we reported a novel fluorescent chemosensor (PPL1) to detect cyanide ions in highly alkaline solutions with a unique and instant fluorescence response (dull green to bright yellow) with great selectivity and sensitivity. The 1:1 binding fashion of PPL1 with CN^- was signified by Job analysis. The limit of detection (0.49 µM) and the interference assay of PPL1 certified its selectivity and suitability for the detection of CN^- in actual samples. The selectivity of PPL1 towards CN^- was ascribed to the deprotonation followed by nucleophilic addition mechanism as proven by ¹H and ¹³C NMR and LC-MS. The PPL1 demonstrated exceptional performance even under extreme pH circumstances and enabled us to quantify cyanide in alkaline water samples in line with the US EPA standard cyanide estimation protocol and in food samples such as sprouting potatoes, cassava, and almonds. The DFT/TD-DFT computations that shed light on the electronic transitions and interaction sites involved in the sensing process robustly validate our experimental findings and proposed mechanism. The PPL1 emerges as an advancing candidate for environmental monitoring and food safety applications due to its strong mechanistic validation (deprotonation followed by nucleophilic addition), low detection limit (0.49 µM), rapid response time (instant response), and swift fluorescence switching with CN^- ions (green to yellow instantaneously in contact with CN^-).</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-Infrared-Absorbing B-N Lewis Pair-Functionalized Anthracene Derivatives: Electronic Structure and Second-Order NLO Properties.","authors":"Cheng Ma, Lijing Gong, Dachuan Chen, Xiangyu Zhang, Huan Guo","doi":"10.1007/s10895-025-04456-y","DOIUrl":"https://doi.org/10.1007/s10895-025-04456-y","url":null,"abstract":"<p><p>Creating optical materials with linear and nonlinear properties has always been a focus of attention in the scientific community. Recently, Pf (x) displays unusual photophysical properties, such as near-infrared absorption and emission, and transparency in the visible light region, etc. However, from the perspective of improving material performance, its structure-property correlation remains to be fully investigated. Herein, three anthracene derivatives are designed based on Pf(x). By means of density functional theory and time-dependent density functional theory, the electronic structure and nonlinear optical response of Pf(x) and three newly designed anthracene derivatives have been thoroughly explored. This investigation indicates that they show narrow energy gap, near-infrared absorption, large first hyperpolarizability, strong polarization resonance for frequency-dependent hyperpolarizabilities, and derivative 3 is a bipolar transport material. With the aid of depolarization analysis, the unit sphere representation method and the hyperpolarizability density analysis, the origin of nonlinearity was systematically analyzed from both structural and physical perspectives. Due to their large first hyperpolarizability, the investigated derivatives may be referred to as superior second-order nonlinear optical materials and widely used in optoelectronic materials.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Time-resolved Fluorescence Immunochromatographic Strip for the Diagnosis of Avian Infectious Bronchitis Virus.","authors":"Laiqing Li, Yanhong Lu, Hongrui Lai, Huankun Liang, Junhao Li, Cuicui Chen, Qiang Jia","doi":"10.1007/s10895-025-04483-9","DOIUrl":"https://doi.org/10.1007/s10895-025-04483-9","url":null,"abstract":"<p><p>Avian infectious bronchitis virus (AIBV) is the main pathogen of avian infectious bronchitis (AIB), causing significant economic losses in the poultry industry worldwide. A novel time-resolved fluorescence immunochromatographic (TRFIM) strip for the diagnosis of AIB was developed via sensitive and quantitative detection of AIBV antigen. In this study, a two-steps TRFIM strip based on europiumion (Eu) fluorescence microspheres was first developed for sensitive and quantitative detection of AIBV antigen. The AIBV-MAb usage, T line MAb concentration, Eu-AIBV-MAb complex usage and reaction time were optimized to improve the detection performance and improve the on-site detection efficiency. Finally, prepared and assembled the strips according to the optimal conditions, and then evaluate their sensitivity, specificity, precision, stability, and real sample detection performance. A two-steps TRFIM strips were prepared and assembled, and their sensitivity was 0.17 ng/mL, with high specificity for AIBV. The reference interval of the TRFIM strips is > 0.42 ng/mL, the recoveries of the intra-batch and inter-batch samples ranged from 88.50 to 97.35%, and all coefficient of variations (CVs) were less than 10%. The period of validity is more than 12 month at room temperature. The performance of the TRFIM strips in real sample detection is comparable to RT-PCR. Therefore, the developed strips have high sensitivity, accuracy, specificity and stability for detection of chicken AIBV antigen, exhibiting potential for broad applications in the on-site surveillance of AIBV in the poultry industry.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesize of Tri-Metal Oxide MnO₂-ZnO-CeO₂ Nanocomposites via Co-Precipitation Technique for Biomedical and Environmental Applications.","authors":"Anugraha Ganapathy Rama Subramanian, Balu Thangaraj, Nagarajan Muthukurumban, Parvathiraja Chelliah, Sree Devi Rethnamuthu","doi":"10.1007/s10895-025-04449-x","DOIUrl":"https://doi.org/10.1007/s10895-025-04449-x","url":null,"abstract":"<p><p>Tri-metallic MnO₂-ZnO-CeO₂ nanocomposites were successfully synthesized via a co-precipitation method. Structural characterization confirmed the formation of orthorhombic MnO₂, hexagonal ZnO, and cubic CeO₂ phases, with an average crystallite size of approximately 18 nm. The nanocomposites exhibited a narrow band gap of 2.36 eV, enabling efficient visible-light absorption and enhanced photocatalytic activity. FTIR analysis confirmed M-O stretching vibrations, while photoluminescence spectra revealed defect-related emission bands attributed to newly formed energy levels. Nitrogen adsorption-desorption isotherms indicated a high surface area with a uniform mesoporous structure, featuring a pore diameter centered at 2.450 nm. HR-TEM analysis showed spherical particles with a coarse, granular nanostructure and an average particle size of ~ 37 nm. Elemental mapping and XPS confirmed the presence and oxidation states of Mn, Zn, Ce, and O. Electrochemical studies revealed significant pseudocapacitive behavior, with a maximum areal capacitance of 111.26 mF/cm² at 5 mV/s and minimal charge transfer resistance. Antibacterial assays demonstrated strong activity against Staphylococcus aureus, with a maximum inhibition zone of 14 mm. Cytotoxicity evaluation showed a concentrate dependent response, reducing MCF-7 cell viability to 12% at 1000 µg/mL. Photocatalytic degradation of methylene blue reached 89.8% within 40 min, following pseudo-first-order kinetics. The effects of pH, catalyst dosage, and dye concentration were systematically examined, and reusability tests confirmed the catalyst stability. These results underscore the potential of MnO₂-ZnO-CeO₂ nanocomposites for effective wastewater treatment applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics Classifications, Toxicity, and Visual Detection Methods Based on Fluorescent Materials.","authors":"Kawan F Kayani, Sewara J Mohammed, Khanda F M Amin, Muhammad S Mustafa","doi":"10.1007/s10895-025-04500-x","DOIUrl":"https://doi.org/10.1007/s10895-025-04500-x","url":null,"abstract":"<p><p>Microplastics (MPs) have emerged as a significant global environmental concern over recent decades due to their widespread presence in oceans, bioavailability, and ability to transport toxic substances. The analysis of MPs in both environmental and experimental samples has become increasingly common. Among the analytical steps, detection is crucial, as it directly provides the results. With a growing preference for simple, rapid, and instrument-free methods, visual detection where results can be seen and interpreted by the naked eye has become essential. This critical review summarizes and discusses advanced visual detection techniques, emphasizing their potential for on-site microplastic monitoring based on carbon dots (CDs), nanozyme sensing assays, and fluorescent dyes. Finally, the review outlines key challenges and proposes potential future research directions in this field.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anchoring Group Engineering in Coumarin-Benzothiazole Sensitizers: A First-Principles Perspective for DSSCs.","authors":"Rinki Deka, Dhruba Jyoti Kalita","doi":"10.1007/s10895-025-04481-x","DOIUrl":"10.1007/s10895-025-04481-x","url":null,"abstract":"<p><p>This study explores the potential of six novel metal-free organic dyes with a D-π-A architecture for use in dye-sensitized solar cells (DSSCs). All dyes share a common diethylamino coumarin donor and benzothiazole π-spacer, but differ in their acceptor/anchoring groups: acetic acid (A1), benzoic acid (A2), 2-cyanoacrylic acid (A3), rhodanine-3-acetic acid (A4), (chlorotetrahydroquinolinyl)cyanoacrylic acid (A5), and (oxotetrahydroquinolinyl)cyanoacrylic acid (A6). Using density functional theory (DFT) and time-dependent DFT (TD-DFT) with the B3LYP-D3 functional and 6-31G(d,p) basis set, we investigated the dyes' photophysical and photoelectrochemical properties. The investigation focused on tailoring the molecular structure to enhance acceptor strength and evaluating the ease of adsorption onto TiO[Formula: see text]. Geometric, structural, electrical, and optical properties, along with charge transfer processes, have been analyzed using quantum chemical methods. The results suggest that these dyes are promising candidates for optoelectronic device applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Interaction of the Coumarin Derivative Umbelliferone with Cu/Zn-SOD1 and DNA: Insights into Binding Mode and Antioxidant Mechanism.","authors":"Duygu İnci Özbağcı, Sevinç İlkar Erdağı, Rahmiye Aydın","doi":"10.1007/s10895-025-04455-z","DOIUrl":"https://doi.org/10.1007/s10895-025-04455-z","url":null,"abstract":"<p><p>A member of the coumarin family, umbelliferone is a naturally abundant compound known for its diverse pharmacological and therapeutic properties. Therefore, to gain insight into its molecular recognition mechanism, we plan to conduct an in-depth investigation of its interactions with calf thymus DNA (CT-DNA) and Cu/Zn superoxide dismutase enzyme (SOD1) through a combination of spectroscopic techniques and computational modeling. Results from competitive displacement experiments with EB and Hoechst 33,258, supported by molecular docking analyses, indicate that the umbelliferone preferentially binds to the minor groove of CT-DNA. Using electronic absorption and fluorescence spectroscopy, we confirmed the interaction between the umbelliferone and SOD1, indicating complex formation. The spontaneous nature of the reaction and the stabilization of the complex via van der Waals forces and hydrogen bonding were confirmed through thermodynamic studies. The study revealed that energy transfer takes place efficiently, with a high probability. Microenvironmental and conformational changes in SOD1 were induced by the umbelliferone, as demonstrated by synchronous spectra, 3D fluorescence maps and FTIR analysis. Molecular docking simulations revealed that the umbelliferone preferentially bind to the minor groove of DNA, forming several hydrogen bonds and van der Waals contacts without disrupting the helical structure. In contrast, the compound interacted with a non-catalytic surface region of SOD1, away from the metal-coordinating active site, suggesting a potential allosteric or stabilizing effect rather than direct enzymatic inhibition. The binding affinities were calculated as - 6.32 kcal/mol for DNA and - 5.70 kcal/mol for SOD1. Furthermore, in silico ADMET analyses indicated high gastrointestinal absorption, blood-brain barrier permeability, and a generally favourable pharmacokinetic profile with acceptable toxicity limits. These findings support the dual role of the umbelliferone as a groove-binding DNA stabilizer and a non-covalent modulator of antioxidant enzymes, highlighting its therapeutic relevance in oxidative stress-related conditions. Additionally, the umbelliferone's antioxidant potential was evaluated through the DPPH assay, demonstrating its capacity to scavenge DPPH free radicals.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvatochromic, DFT Insight into Chemical and Physical Parameters of 4-(4-Nitro-phenoxymethyl)-benzo[h]chromen-2-one (NM2BC).","authors":"Shivakumar Chillargikar, Srinath, S M Hanagodimath, Omnath Patil, Sulochana Dever","doi":"10.1007/s10895-025-04465-x","DOIUrl":"https://doi.org/10.1007/s10895-025-04465-x","url":null,"abstract":"<p><p>The absorption and fluorescence characteristics of coumarin derivative 4-(4-Nitro-phenoxymethyl)-benzo[h]chromen-2-one (NM2BC) are studied at room temperature in pure organic solvents to understand the nature of its electronic transitions and solvent interactions. The study aims to investigate the solvent-dependent photophysical behavior of NM2BC and estimate the electric dipole moments in both the ground and excited states using solvatochromic methods. Additionally, the work seeks to explore the intramolecular charge transfer (ICT) nature in the excited state and assess the chemical reactivity and stability of NM2BC. The effects of solvents on spectral characteristics were examined using solvatochromic techniques, and Density Functional Theory (DFT) at the CAM-B3LYP/6-311 + + G (d, p) level was utilized in conjunction with quantum chemistry calculations and the solvatochromic shift to estimate electric dipole moments in both the ground and excited states. The electronic structure was analyzed using Frontier Molecular Orbital (FMO) analysis. To evaluate reactivity and stability, Global Reactivity Descriptors were calculated using FMOs. To determine the preferred electrophilic and nucleophilic attack sites, Molecular Electrostatic Potential (MEP) analysis was performed. Strong interactions and intramolecular charge delocalization were investigated using Natural Bond Orbital (NBO) analysis.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A NIR Isophorone-based Zinc Coordination Fluorescence Probe for the Detection of Glyphosate and Application in Environment, Food and Living Systems.","authors":"Si-Yi Yao, Han-Wen Cui, Zhi-Bo Jiang, Hao Wang, Jing-Yi Li, Yu-Yang Wang, Fan-Qi Shen, Li-Xia Zhao, Ya-Tong Liu, Yue-Li Zou","doi":"10.1007/s10895-025-04470-0","DOIUrl":"https://doi.org/10.1007/s10895-025-04470-0","url":null,"abstract":"","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Novel Core-Shell ZIF-8@poly L-DOPA Nanoparticles for Targeted Doxorubicin Delivery.","authors":"Mohammad Amin Damyar, Azadeh Serri, Hamid Hashemi-Moghaddam, Farzad Kahrizi","doi":"10.1007/s10895-025-04506-5","DOIUrl":"https://doi.org/10.1007/s10895-025-04506-5","url":null,"abstract":"<p><p>Drug delivery systems using nanoparticles can deliver to tumor cells without affecting normal cells. In this study, a novel nano composite was synthesized by coupling abilities of ZIF-8 MOF and poly L-DOPA molecular imprinted polymer. Thus, a selective drug delivery system was designed for potential cancer treatment. A new nanocomposite, poly 3,4-dihydroxy-L-phenylalanine/ZIF-8 (L-DOPA/ZIF-8), was synthesized and used for targeted Doxorubicin delivery. The structural, morphological, and fluorescence properties of the synthesized samples were investigated by FT-IR, FE-SEM, and spectrofluorimetry. The investigation and characterization of the prepared ZIF-8@poly(dopa)/DOX sample indicated its layered and porous structure. The fluorescence emission spectrum showed that adding doxorubicin led to an increase in emission intensity, marking the successful loading of DOX. The safety and cytotoxicity of the prepared nanocomposite were investigated using the MTT method using the HEK293 cell line. The results show that the nanocomposite can be a biocompatible and safe nanocarrier in drug delivery applications. The drug release rate was investigated at two different pH levels, 7.4 and 5. The cumulative or total release of DOX from ZIF-8@poly(dopa)/DOX nanocomposite at pH 7.4 and 5.4 is 56.7 and 78.4%, respectively. Thus, this nanocarrier can be a suitable carrier for slow and controlled drug release into cancer cells.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}