Journal of Fluorescence最新文献_第3页

A New Fluorescent Method for Measuring Peroxiredoxin Enzyme Activity Using Monobromobimane. 利用单溴比曼测量过氧化氢酶活性的新型荧光方法

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-23 DOI: 10.1007/s10895-024-03991-4

Nawar Yaseen Mohsin, Halit Demir, Mahmoud Hussein Hadwan, Asad M Hadwan, Rawaa M Mohammed

{"title":"A New Fluorescent Method for Measuring Peroxiredoxin Enzyme Activity Using Monobromobimane.","authors":"Nawar Yaseen Mohsin, Halit Demir, Mahmoud Hussein Hadwan, Asad M Hadwan, Rawaa M Mohammed","doi":"10.1007/s10895-024-03991-4","DOIUrl":"https://doi.org/10.1007/s10895-024-03991-4","url":null,"abstract":"A novel fluorometric method is presented for accurately quantifying peroxiredoxin (Prx) enzyme activity in vitro. The rate-limiting step in the Prx-catalyzed reaction is the dissociation of peroxide. To avoid interference from catalase, we developed an assay using tert-butyl hydroperoxide (t-BOOH) as a substrate for specific Prx activity measurement. The assay involves incubating the enzyme substrates 1,4-dithio-DL-threitol (DTT) and t-BOOH in a suitable buffer at 37 °C for 10 min in a known volume of Prx enzyme. Following incubation, the reagent monobromobimane (mBB) is added to terminate the enzymatic reaction and produce a fluorescent product. Prx activity is subsequently determined by measuring thiol fluorescence, with reaction conditions optimized using a Bland-Altman plot. The efficacy of this novel protocol was rigorously validated by comparing Prx activity measurements from paired samples with those generated by a reference assay. A correlation coefficient of 0.995 was observed between the two methods, demonstrating superior precision and reliability compared to existing methods. The mBB-Prx protocol offers a significant safety advantage by using t-BOOH as a substrate for Prx activity measurement. As catalase does not catalyze t-BOOH dissociation, including sodium azide is unnecessary. Moreover, the method obviates the need for concentrated acids to terminate the Prx enzymatic reaction, as the mBB reagent efficiently inhibits Prx activity. This streamlined approach simplifies the assay and significantly improves its safety and usability, providing users with a reliable and convenient tool. The convenience of this method allows users to focus on their research without worrying about safety or complex procedures.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen and Sulfur Co-doped Carbon Dots for Ratiometric Fluorometric Determination of Mercury Ions. 用于汞离子比率荧光测定的氮和硫掺杂碳点

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-23 DOI: 10.1007/s10895-024-04010-2

Fenglan Li, Liqin Lu, Yutong Wu, Liang Meng, Binling Zhu, Quanming Xu, Guoxin Zhuang, Junyang Zhuang

引用次数: 0

Design and In Vitro Evaluation of Fluorescent MOF-Core CaCO₃-PEI-FA Shell Nanoparticles for Targeted Therapy of Laryngeal Cancer Cells. 用于喉癌细胞靶向治疗的荧光 MOF-Core CaCO₃-PEI-FA 外壳纳米粒子的设计与体外评估

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-23 DOI: 10.1007/s10895-024-04013-z

Hongmei Zhu, Bo Yang, Yang Niu, Yongjiu Huang

{"title":"Design and In Vitro Evaluation of Fluorescent MOF-Core CaCO₃-PEI-FA Shell Nanoparticles for Targeted Therapy of Laryngeal Cancer Cells.","authors":"Hongmei Zhu, Bo Yang, Yang Niu, Yongjiu Huang","doi":"10.1007/s10895-024-04013-z","DOIUrl":"https://doi.org/10.1007/s10895-024-04013-z","url":null,"abstract":"Laryngeal cancer, a common malignant respiratory tumor, is primarily treated through surgery. However, challenges such as recurrence, metastasis, and drug resistance persist. In recent years, multifunctional drug delivery systems (DDS) based on nanoparticles have shown great potential in improving drug loading and release. We developed a biocompatible core-shell nanoparticle system with a zinc-based metal-organic framework (MOF) as the core, named CP1. The shell, composed of polyethyleneimine (PEI), folic acid, and calcium carbonate, forms a composite called CaCO3-PEI-FA. This system enhances biocompatibility and increases the efficacy of biomedical applications. Encapsulating CP1 within the CaCO3-PEI-FA shell allows for the targeted delivery of the anticancer drug doxorubicin (DOX) to laryngeal cancer cells (Hep-2), resulting in the CaCO3-PEI-FA@CP1@DOX system. The CaCO3-PEI-FA composite exhibits strong fluorescence with a peak around 350 nm, confirming successful synthesis and demonstrating its potential as a bioimaging probe. Importantly, the nanoparticle system without DOX showed low toxicity to normal human skin fibroblasts (HSF). In vitro cytology experiments revealed a 38% inhibition rate of Hep-2 cells after 24 h, highlighting the nanocomposite's significant potential in inhibiting laryngeal cancer cell proliferation and inducing apoptosis, underscoring its promise in targeted laryngeal cancer therapy.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Mitochondria-Targeting Water-Soluble Fluorescent Probe for Selective Detection of Glyoxal in Living Cells. 用于选择性检测活细胞中乙二醛的线粒体靶向水溶性荧光探针。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-23 DOI: 10.1007/s10895-024-03994-1

Zhiming Wang, Chang Liu, Yuanyuan Wu, Huirong Yao, Song He, Liancheng Zhao, Xianshun Zeng

引用次数: 0

Two Squaramide-Based Fluorescent Probes for Cu²⁺ and Cd². 两种基于 Squaramide 的 Cu2+ 和 Cd2 荧光探针。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-21 DOI: 10.1007/s10895-024-03956-7

Yuanwei Li, Hongxu Wang, Qian Tao, Bin Wang

引用次数: 0

Study of Luminescence Phenomena of Tb (III) Containing Fluroquinoline for Application in Optoelectronic Devices. 研究含 Tb (III) 的氟喹啉在光电设备中的发光现象。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-21 DOI: 10.1007/s10895-024-03989-y

Poonam Kumari, Vaishnavi Lather, Arjun Malik, Pratibha Ahlawat, Rajesh Kumar

{"title":"Study of Luminescence Phenomena of Tb (III) Containing Fluroquinoline for Application in Optoelectronic Devices.","authors":"Poonam Kumari, Vaishnavi Lather, Arjun Malik, Pratibha Ahlawat, Rajesh Kumar","doi":"10.1007/s10895-024-03989-y","DOIUrl":"https://doi.org/10.1007/s10895-024-03989-y","url":null,"abstract":"This study presents a series of six vivid green Tb(III) complexes, denoted by the general formula [Tb(L)3.secondary sensitizers], where L represents 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxoquinoline-3-carboxylic acid and secondary sensitizers consist of heterocyclic N-donor aromatic systems. The synthesis of these complexes were achieved through a solvent-assisted grinding method, and their characterization involved various techniques such as CHN analysis, FTIR, NMR, UV, XRD, and NIR spectroscopy. These analyses confirmed the successful synthesis of complexes with coordination between the quinoline moiety and the metal ion. Photoluminescence studies were conducted in solid and solution phases, revealing excellent luminescence properties. The bright green color emitted by the complexes upon exposure to UV rays was attributed to the hypersensitive 5D4 → 7F5 transition. J-O analysis indicated an asymmetrical coordination environment around in the complexes. Additionally, various radiative properties (Ared, Anred, η, βexp, σs) and band gap values were determined, highlighting the potential applications of these complexes in diverse optoelectronic fields. Chromaticity evaluation demonstrated high color purity in both solid and solution phases. Furthermore, the CCT value identified the solid complexes as a cool light source. Overall, the analyses supported the exceptional luminosity of synthesized complexes, positioning them as promising luminescent materials for a wide range of devices.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactive Fluorescent and Colorimetric Probe for Highly Selective and Sensitive Detection of Hg²⁺ in Real Water Samples. 用于高选择性和高灵敏度检测真实水样中 Hg2+ 的反应性荧光和比色探针。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-19 DOI: 10.1007/s10895-024-03992-3

Jiarui Yang, Kaiqiang Zhang, Yong Zhao, Yanxi Song, Yihan Wu, Hongqi Li

引用次数: 0

Application of Fluorescent Composite Materials as a Sustained Release System in Treatment of Polycystic Ovary Syndrome. 荧光复合材料作为缓释系统在多囊卵巢综合征治疗中的应用

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-19 DOI: 10.1007/s10895-024-03993-2

Lin Wang, Liangshan Mu, Yao Ye, Jun Xu, Xutong Zou

{"title":"Application of Fluorescent Composite Materials as a Sustained Release System in Treatment of Polycystic Ovary Syndrome.","authors":"Lin Wang, Liangshan Mu, Yao Ye, Jun Xu, Xutong Zou","doi":"10.1007/s10895-024-03993-2","DOIUrl":"https://doi.org/10.1007/s10895-024-03993-2","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a multifactorial disease characterized by oxidative stress and follicular dysfunction, leading to menstrual irregularities, hyperandrogenism, and infertility. Traditional drug delivery methods often result in drug loss and side effects on normal tissues. To address these issues, we synthesized two novel Co(II)-containing coordination polymers (CPs), {[Co(L)(H2O)2]·2H2O}n (1) and {[Co(L)(H2O)2]·1.5H2O}n (2), through the reaction of the T-shaped ligand (4 - 3'-pyridyl-,6 - 4'-carboxylphenyl)picolinic acid (H2L) with Co(NO3)2·6H2O via a solvothermal process. Fluorescence spectroscopy revealed that the fluorescence emission of the CPs originates from the ligand, indicating their potential application as blue fluorescence materials. Subsequently, we encapsulated these CPs with hyaluronic acid (HA) and carboxymethyl chitosan (CMCS) hydrogels to create two types of metal gel particles carrying spironolactone (HA/CMCS-CPs@spironolactone). SEM and TEM analyses showed that the material consists of tightly stacked sheet-like structures with an average size of approximately 100 nm. Thermogravimetric analysis (TGA) indicated that the material begins to decompose at around 115 °C, demonstrating good thermal stability. We assessed the inhibitory effects of these materials on oxidative stress induced by PCOS. The results showed that both types of spironolactone-loaded metal gel particles significantly reduced malondialdehyde (MDA) levels, particularly the particles constructed with CP2. HA/CMCS-CP1@spironolactone reduced MDA levels by approximately 17% and 46% at low and high concentrations, respectively, while HA/CMCS-CP2@spironolactone decreased MDA levels by about 55% and 39% at high and low concentrations, respectively. Therefore, the novel drug delivery system reported in this study has the potential to become a safe and effective option for the localized treatment of PCOS.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent Determination of Uric Acid Based on Porphyrin and ZnCo₂O₄ Nanocomposite. 基于卟啉和 ZnCo2O4 纳米复合材料的尿酸荧光测定。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-19 DOI: 10.1007/s10895-024-03986-1

Shijo Francis, Shamna Salim, Leena Rajith

{"title":"Fluorescent Determination of Uric Acid Based on Porphyrin and ZnCo2O4 Nanocomposite.","authors":"Shijo Francis, Shamna Salim, Leena Rajith","doi":"10.1007/s10895-024-03986-1","DOIUrl":"https://doi.org/10.1007/s10895-024-03986-1","url":null,"abstract":"Advances in porphyrin chemistry have provided exciting technologies in the field of optical biosensing. Herein, we have synthesized 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin (TCPP) and porous Zn0.1Co2O4 nanorods using a simple one-pot hydrothermal method. The obtained TCPP- Zn0.1Co2O4 composite was then used for the development of a novel optical sensor for the determination of uric acid (UA), which is an important biomarker in human urine, serum or saliva for the clinical diagnosis of hyperuricemia and hypouricemia, etc. TCPP-Zn0.1Co2O4 composite was characterized using SEM, TEM, EDAX, PXRD, FT-IR, UV-Visible, and NMR spectroscopic techniques. The fluorescence emission spectral analysis of TCPP-Zn0.1Co2O4 was then investigated for potential applications in the detection of uric acid via the fluorescence quenching mechanism. The designed sensor showed a linear response towards the uric acid in the concentration range of 0.99 to 5.2 nM. The optical sensor exhibits a sensitive response to uric acid with a detection limit of 0.015 nM. The sensor was employed to quantify UA in spiked human urine samples and artificial urine with satisfactory results.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utility of Microwave-Synthesized Silver Nano Particles as Spectrofluorimetric Sensors for the Determination of Nano Concentrations of Favipravir: Application to Dosage Forms and Spiked Human Plasma. 微波合成的银纳米颗粒作为光谱荧光传感器测定法维拉韦纳米浓度的实用性：应用于剂型和加标人体血浆。

IF 2.6 4区化学

Journal of Fluorescence Pub Date : 2024-10-19 DOI: 10.1007/s10895-024-03979-0

Mona H Abo Zaid, Nahed El-Enany, Aziza E Mostafa, Ghada M Hadad, Fathalla Belal

{"title":"Utility of Microwave-Synthesized Silver Nano Particles as Spectrofluorimetric Sensors for the Determination of Nano Concentrations of Favipravir: Application to Dosage Forms and Spiked Human Plasma.","authors":"Mona H Abo Zaid, Nahed El-Enany, Aziza E Mostafa, Ghada M Hadad, Fathalla Belal","doi":"10.1007/s10895-024-03979-0","DOIUrl":"https://doi.org/10.1007/s10895-024-03979-0","url":null,"abstract":"A simple and facile microwave-assisted method was developed for the synthesis of highly fluorescent silver-nanoparticles (Ag-NPs). The synthesis of silver-nanoparticles depends on a redox reaction between silver nitrate and ascorbic acid using chitosan as a stabilizing agent. The produced Ag-NPs were characterized using Zeta potential and transmission electron microscope micrograph where they are spherical in shape with smooth surface morphology and size of 26.81 ± 2.2 nm. Favipiravir (FAV) was found to cause an obvious enhancement in the fluorescence of Ag-NPs; hence, they were used for its spectrofluorimetric estimation. The fluorescence intensity was measured at 430 nm after excitation at 360 nm. Under optimum conditions, a good linear relationship was accomplished between the FAV concentration and the fluorescence intensity in a range of (5.0-200.0) ng/mL with a limit of detection of 1.59 ng/mL. The method was successfully applied for the assay of the drug in its commercial tablets and spiked human plasma samples, and the results obtained were satisfactory.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0