Journal of Fluorescence最新文献

{"title":"Coumarin 153 Fluorescent Probe for Highly Sensitive and Selective Detection of 2-Nitrodiphenylamine.","authors":"Ting Wang, Yuanyu Tang, Tingting Lei, Shaojing Zhao, Xiaona Li, Chengyuan Zhao, Fengtao Zhang, Xuhui Chi, Minhuan Lan","doi":"10.1007/s10895-026-04956-5","DOIUrl":"https://doi.org/10.1007/s10895-026-04956-5","url":null,"abstract":"<p><p>During propellant storage, the release of nitrogen oxides consumes the stabilizer 2-nitrodiphenylamine (2-NDPA), whose residual content serves as a key indicator for assessing ammunition aging and safe service life. Conventional detection methods, predominantly based on chromatography, are hindered by high costs, complex operation, and unsuitability for on-site analysis. Herein, we report a rapid detection method using coumarin 153 (C153) as a single fluorescent signal unit. Experimental results show that the fluorescence intensity of C153 at 527 nm decreases linearly upon addition of 2-NDPA, with a rapid response (response time < 5 s) and a detection limit of 0.60 µmol·L^-1. Selectivity tests against 19 structurally analogous stabilizers and their derivatives confirm the high specificity of this probe for 2-NDPA. Mechanistic investigations, combining fluorescence lifetime measurements and spectral overlap analysis, attribute the quenching to the inner filter effect. Furthermore, the practical utility of this method is validated through both leachate and vapor-phase tests on aged propellant samples, demonstrating that the fluorescence quenching response correlates inversely with the extent of aging. This strategy offers a cost-effective and operationally simple alternative for monitoring propellant stabilizers.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Mode Fluorescence/UV-Vis Sensing Platform Based on Luminescence Modulation of Upconversion Nanoparticles.","authors":"Leila Khaleghi, Nahid Sarlak","doi":"10.1007/s10895-026-04951-w","DOIUrl":"https://doi.org/10.1007/s10895-026-04951-w","url":null,"abstract":"<p><p>Upconverting nanoparticles (UCNPs) composed of sodium yttrium fluoride (NaYF₄) nanocrystals doped with trivalent rare-earth ions are synthesized by a co-precipitation method. Under 980 nm excitation, the UCNPs exhibit two prominent emission bands, both of which undergo significant quenching upon introduction of lycopene. Laser-induced fluorescence measurements reveal a pronounced decrease in luminescence intensity following lycopene addition. UV-visible absorption and steady-state fluorescence spectroscopy further demonstrate a linear correlation between lycopene concentration and signal attenuation. The limit of detection (LOD) for the UV-Vis assay is 328 ppm, while fluorescence detection attains an LOD of 234.5 ppm. Correlation coefficients (R²) of 0.9931 (UV-Vis) and 0.9958 (fluorescence) and relative standard deviations (RSD) of 3.9% and 1.76%, respectively, indicate high analytical performance. Quenching behavior is rationalized in terms of energy transfer mechanisms, underscoring the potential of UCNP-lycopene systems for optical sensing applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149061955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ratiometric Fluorescent Sensing of MMP-3 via FRET for Postoperative Rehabilitation Assessment and Prognostic Monitoring Following Joint Surgery.","authors":"Letao Jiang, Kai Feng, Yu Zhang, Yi Zhou, Shang Wu, Jianwei Du, Jing Wang","doi":"10.1007/s10895-026-04945-8","DOIUrl":"https://doi.org/10.1007/s10895-026-04945-8","url":null,"abstract":"<p><p>Matrix metalloproteinase-3 (MMP-3) is a critical biomarker for inflammatory responses and tissue remodeling following joint surgery. Here, we developed a ratiometric fluorescence probe based on fluorescence resonance energy transfer (FRET) for sensitive and selective detection of MMP-3 activity. The probe comprises CdTe quantum dots (R-QDs) as the energy donor and BHQ as the acceptor, linked by an MMP-3-cleavable peptide. Upon MMP-3 cleavage, the FRET process is disrupted, resulting in fluorescence recovery at 540 nm, while the reference signal at 690 nm remains constant, enabling ratiometric quantification. The probe exhibits a linear response from 20 to 100 nM with a detection limit of 7.8 nM and excellent selectivity against other MMPs and interfering proteins. Clinical validation using serum samples from 11 subjects (5 healthy controls and 6 post-surgery patients) successfully distinguished patients from controls. Moreover, real-time monitoring of 6 patients over 18 days post-surgery revealed a clear correlation between MMP-3 levels and rehabilitation progress. These results demonstrate that our ratiometric FRET probe holds promise for postoperative rehabilitation assessment and prognostic monitoring following joint surgery.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A High-affinity Near-infrared Fluorescent Probe for Interference-resistant Cu²⁺ Determination in Real Samples and Living Plant Imaging.","authors":"Yuqian Wu, Luchao Zhang, Lijing Guo, Yunfei Luo, Mengjiao Wang, Dongxiang Zhao, Gui Gao, Ruixin Wu","doi":"10.1007/s10895-026-04937-8","DOIUrl":"https://doi.org/10.1007/s10895-026-04937-8","url":null,"abstract":"<p><p>Copper ions (Cu²⁺) play important roles in crop growth and agricultural production, but excessive Cu²⁺ accumulation in crops and their growth environments may pose risks to crop quality, food safety, and human health. In view of increasing copper contamination in agricultural environments, developing rapid, sensitive, and reliable methods for Cu²⁺ determination in real agricultural matrices is highly desirable. In this work, a high-affinity near-infrared (NIR) fluorescent probe, DCI-Cu, was successfully developed for interference-resistant Cu²⁺ determination. The probe exhibited NIR emission at 670 nm, a large Stokes shift of approximately 200 nm, broad pH tolerance, favorable photostability, high selectivity, and strong anti-interference capability toward common coexisting species, especially sulfur-containing species, biological thiols, and ethylenediaminetetraacetic acid (EDTA). Benefiting from these properties, DCI-Cu enabled ultrafast and sensitive Cu²⁺ detection with a fluorescence \"turn-off\" response within 3 s and a low detection limit of 136 nM. Mechanistic studies supported a 1:1 binding model between DCI-Cu and Cu²⁺, and the high coordination constant of 8.42 × 10⁷ M⁻¹ was associated with a proposed six-coordinate coordination model. DCI-Cu was successfully applied to accurate quantitative detection of Cu²⁺ in real water, soil, and crop samples, with satisfactory recoveries of 96.5%-105.5% and low RSDs of 1.43%-2.51%. Moreover, DCI-Cu was successfully used for in situ fluorescence imaging of Cu²⁺-related changes in living triticale leaves. Consequently, DCI-Cu provides a promising NIR fluorescence tool for rapid, sensitive, and interference-resistant Cu²⁺ analysis in crop-associated environmental and plant matrices.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149061865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A \"turn-on\" Fluorescent Probe with Ions-induced ESIPT for the Detection of Cd²⁺ and Zn².","authors":"Mei Yu, Nana Mu, Qin He, Jianhua Hu, Jiangqin Liang, Zheng Chen, Zhiwei Lu, Liang Zhao, Tianzhu Shi, Ju Guo, Qulai Tang, Fuyong Wu, Long Chen, Qiong Tang","doi":"10.1007/s10895-026-04944-9","DOIUrl":"https://doi.org/10.1007/s10895-026-04944-9","url":null,"abstract":"<p><p>Developing an Aggregation-Induced Emission Enhancement (AIEE) type probe with enhanced emission through metal ion-induced methods has been a significant challenge. In this work, we synthesized an AIEE fluorescent chemosensor P1 via a Schiff base reaction by 8-hydroxyquinoline-2-carbaldehyde and 2,4-difluorophenylhydrazine. Experimental results demonstrate that chemosensor P1 exhibits AIEE behavior in DMSO/glycerol mixed solvents. Significantly, this probe exhibits multifunctionality in ethanol solution: (1) specific recognition of Zn²⁺/Cd²⁺ ions against other cations; (2) colorimetric discrimination of concentration-dependent under white light; (3) metal ion-triggered red luminescence observed in the solution is similar to the characteristic emission signatures of the excited-state intramolecular proton transfer (ESIPT)-driven molecules in methanol. The system demonstrates good linear relationships between fluorescence intensity and ion concentration within specific ranges in ethanol: For Cd²⁺: 4.0 × 10⁻⁷-2.2 × 10⁻⁶ mol·L⁻¹ with a detection limit of 9.11 × 10⁻⁸ mol·L⁻¹; For Zn²⁺: 2.0 × 10⁻⁷-2.0 × 10⁻⁶ mol·L⁻¹ with a detection limit of 1.02 × 10⁻⁷ mol·L⁻¹. Thus, chemosensor P1 is capable of detecting both Cd²⁺ and Zn²⁺ with significant red fluorescence enhancement, which establishes a new strategy for designing ESIPT Schiff-base probes.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Selective and Visual Detection of Co²⁺ Using CDs/MOF-Eu Dual-Wavelength Fluorescent Hydrogels.","authors":"Zhongli Wu, Xiaomin Jian, Wenjing He, Kaicheng Zhou, Xingjun Li, Xinchi Duan, Daohai Zhang","doi":"10.1007/s10895-026-04950-x","DOIUrl":"https://doi.org/10.1007/s10895-026-04950-x","url":null,"abstract":"<p><p>Contamination by the heavy metal cobalt ion (Co²⁺) poses a serious threat to the ecological environment and human safety. Consequently, there is an urgent need to develop a new method for detecting Co²⁺ that combines rapid response, high sensitivity and high selectivity. In this study, carbon dots (CDs) were prepared using urea and citric acid as raw materials. These were mixed with MOF-Eu and incorporated into a PA hydrogel constructed from sodium alginate (SA) and acrylamide (PAM), resulting in the successful preparation of a PA-CDs@MOF-Eu composite hydrogel with dual-wavelength independent luminescence properties. The results indicate that the hydrogel exhibits characteristic red fluorescence from MOF-Eu upon excitation at 398 nm and characteristic blue fluorescence from CDs upon excitation at 342 nm; precise control of the dual wavelengths can be achieved by adjusting the excitation wavelength. Furthermore, the hydrogel enables highly sensitive and selective visual detection of Co²⁺, with a detection limit (LOD) of 0.22 µM within a linear response range of 0 ~ 120 µM. The fluorescence quenching mechanism of the hydrogel upon introduction of Co²⁺ is attributed to electronic energy transfer (EET). This study provides a new, highly efficient and stable material for the detection of heavy metal ions, offering significant potential for application in fields such as water quality testing and environmental monitoring.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An \"On-Off-On\" Fluorescence Sensing System Based on Nitrogen-Doped Carbon Quantum Dots (N-CQDs) and Ferric Ions (Fe³⁺) for the Determination of Captopril (CAP) in Real Samples.","authors":"Shenyi Ye, Jingrui Cai, Ruyu Ding, Hang Zhu, Jiecheng Xiao, Tianhui Liu, Chengfei Zhao","doi":"10.1007/s10895-026-04911-4","DOIUrl":"10.1007/s10895-026-04911-4","url":null,"abstract":"<p><p>In this study, nitrogen-doped carbon quantum dots (N-CQDs) are synthesized from citric acid and tetraethylenepentamine via a one-step melting method. Leveraging the fluorescence quenching of N-CQDs by Fe³⁺ through a combined static quenching and inner filter effect mechanism, an \"on-off-on\" fluorescent sensing platform was constructed for the detection of captopril (CAP). The method relies on the competitive chelation of Fe³⁺ by the thiol and carboxyl groups of CAP, which displaces the metal ions from the N-CQD surface and restores the quenched fluorescence. Under optimized conditions, the sensor exhibited a detection limit of 0.0620 mmol/L and a linear response spanning 0.1-1 mmol/L. The method was successfully applied to the determination of CAP in commercial tablet formulations, with recoveries meeting the acceptance criteria of the Chinese Pharmacopoeia (2020 Edition). The proposed approach offers a simple, rapid, and sensitive strategy for the quantitative analysis of CAP in pharmaceutical products.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"5399-5420"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated SARA-Fluorescence Analysis of Crude Oil with Fraction-Specific Calibration.","authors":"Azhar Kamel, Sagida Abass Hussain, Nasser M Hadi, Jalal N Jeber","doi":"10.1007/s10895-026-04919-w","DOIUrl":"10.1007/s10895-026-04919-w","url":null,"abstract":"<p><p>The compositional complexity and variability of crude oil demand rapid, reliable analytical methods for both refinery optimization and environmental forensics. Fluorescence spectroscopy is a sensitive, non-destructive alternative to conventional chromatographic and thermal techniques, yet its potential for quantitative, fraction-specific analysis remains under-exploited. In this work, SARA (saturates, aromatics, resins, asphaltenes) fractionation was integrated with systematic fluorescence profiling to characterize Iraqi Basra crude oil. Each fraction exhibited a distinct, reproducible fluorescence signature reflecting its underlying photophysics: the emission intensity of the saturate (alkane) and aromatic fractions increased linearly with concentration (0.001-0.04% w/v and 0.001-0.02% w/v, respectively; R² > 0.998), whereas the resin and asphaltene fractions displayed inverse linear responses (0.005-0.04% w/v and 0.005-0.08% w/v, respectively; R² > 0.997) attributable to aggregation-induced quenching. Fraction-specific linear calibration equations were established, and their slopes, intercepts, 95% confidence intervals, limits of detection and limits of quantification are reported. A systematic bathochromic shift in the emission maxima, from the saturate fraction (388 nm) and aromatics (435 nm) to asphaltenes (455 nm) and resins (527 nm) under 350 nm excitation, provides a spectroscopic ruler for the degree of polycyclic aromatic hydrocarbon (PAH) condensation, as confirmed by sub-fractionation of the aromatic fraction into mono-, bi-, and polycyclic classes (λ_max = 411, 450 and 480 nm, respectively). The method delivered good accuracy (90-105% recovery) and precision (intra-day RSD < 5%). Cross-validation against GC-MS on ten real petroleum samples showed no statistically significant bias (paired t-test, p = 0.75) or difference in precision (F-test, p = 0.78), confirming that the fluorescence-derived aromatic contents are statistically indistinguishable from the reference method. Because the individual SARA signatures overlap in untreated whole crude oil, the method is applied after SARA fractionation. By linking spectral fingerprints to chemical composition, this work advances fluorescence spectroscopy beyond qualitative fingerprinting toward a validated tool for rapid crude oil characterization, with promising potential for future field-deployable applications in spill assessment, pipeline monitoring and refinery feedstock evaluation, pending validation on a broader range of crude oil types.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"5551-5565"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concurrent Enhancement of Aggregation-induced Emission and Two-photon Absorption in Symmetric Naphthalimide Derivatives via Methoxy Substitution.","authors":"Xue Sun, Ying Bao, Haoran Ni, Jiuqi Yang, Dajun Liu, Yi Qu, Yaochuan Wang","doi":"10.1007/s10895-026-04903-4","DOIUrl":"10.1007/s10895-026-04903-4","url":null,"abstract":"<p><p>In this work, bisNAPTPA and its derivative bisNAPTPA-OCH₃ have been investigated to reveal how targeted methoxy substitution tunes the photophysical properties in symmetric naphthalimide derivatives. Combined experimental and theoretical analyses reveal that the introduced methoxy groups at the terminals of triphenylamine groups not only induce distinct aggregation-induced emission (AIE) by restricting intramolecular motions, as confirmed by time-resolved fluorescence, but also significantly enhance intramolecular charge transfer (CT). This enhanced intramolecular CT characteristic leads to a red shift in absorption and a notable improvement in the two-photon absorption (TPA) cross-section. Visualization of excited states via transition density matrix and charge density difference analyses provides a mechanistic understanding of the superior electron delocalization and nonlinear optical response in bisNAPTPA-OCH₃. Our findings suggest that methoxy functionalization represents a promising molecular design motif for simultaneously enhancing AIE characteristics and TPA performance in symmetric naphthalimide derivatives.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"5251-5262"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148684804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric and Fluorometric Recognition of Al³⁺ using Thiazole-Based Schiff base: A Mini-Review.","authors":"Md Shahid, Md Zafer Alam, Salman A Khan","doi":"10.1007/s10895-026-04909-y","DOIUrl":"10.1007/s10895-026-04909-y","url":null,"abstract":"<p><p>The recognition of Al³⁺ ion is significant due to their widespread presence in the environment and their potential toxic effects on human health. Therefore, the identification of these metal ions in various samples is of great importance. This review focuses on the key developments, design strategies, and progress of thiazole-based Schiff base chemosensors for the colorimetric and fluorometric detection of Al³⁺ ion. Thiazole Schiff bases displayed strong coordination affinity toward Al³⁺ through nitrogen, oxygen, and sulphur donor atoms, resulting in observable color variation and fluorescence enhancement. In this review, various thiazole-based Schiff bases are discussed to demonstrate their high selectivity, sensitivity, low detection limits (µM to pM), and rapid response times. The observed optical changes was demonstrated by different mechanisms, including chelation-enhanced fluorescence (CHEF), inhibition of photoinduced electron transfer (PET), intramolecular charge transfer (ICT), and excited-state proton transfer (ESPT), all of which play crucial roles in sensing behavior. Furthermore, several probes exhibit reversible binding and are applicable in environmental water and biological systems. Overall, thiazole-based Schiff base derivatives represent a promising class of colorimetric and fluorescent chemosensors for the detection of minute amounts of Al³⁺ ion, with wide applications in environmental monitoring, biomedical diagnostics, and intracellular imaging.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"5489-5503"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}