Journal of Fluorescence最新文献

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Fluorescent Nitrogen-doped Carbon Dots-based Turn-off Sensor for Bilirubin. 基于荧光掺氮碳点的胆红素关断传感器
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-12 DOI: 10.1007/s10895-024-03771-0
Aswathy A O, B A Akhila, Sony George
{"title":"Fluorescent Nitrogen-doped Carbon Dots-based Turn-off Sensor for Bilirubin.","authors":"Aswathy A O, B A Akhila, Sony George","doi":"10.1007/s10895-024-03771-0","DOIUrl":"10.1007/s10895-024-03771-0","url":null,"abstract":"<p><p>Bilirubin (BR), a heme protein produced from breakdown of haemoglobin is present in aged red blood cells; whose abnormal concentration is associated with diseases like hyperbilirubinemia, coronary disease, iron deficiency, and so on. Herein, we have synthesized a selective, sensitive, and low-cost sensing platform using fluorescent nitrogen doped carbon dots (NCDs), prepared from precursors; citric acid and urea via a simple microwave-assisted method. The emission at 444 nm on excitation with 360 nm was well quenched in presence of BR suggesting a direct turn-off detection for BR. Characterization of developed probe was done by UV-Visible absorption studies, photoluminescence studies, SEM, TEM, ATR-FTIR, XPS, and DLS analysis. BR was detected with a Limit of Detection (LoD) and Limit of Quantification (LoQ) of 0.32 µM and 1.08 µM respectively. NCDs exhibited excellent selectivity and sensitivity towards BR in the presence of co-existing biomolecules and ions. Practical feasibility was checked by paper-strip-based sensing of BR and spiked real human samples were used for conducting real sample analysis.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3565-3576"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent Progress of Fluorescent Carbon Dots and Graphene Quantum Dots for Biosensors: Synthesis of Solution Methods and their Medical Applications. 用于生物传感器的荧光碳点和石墨烯量子点的最新进展:溶液方法的合成及其医学应用》。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-13 DOI: 10.1007/s10895-024-03809-3
Rafaqat Khan, Amina Qureshi, Muhammad Azhar, Zia Ul Hassan, Sagheer Gul, Saeed Ahmad
{"title":"Recent Progress of Fluorescent Carbon Dots and Graphene Quantum Dots for Biosensors: Synthesis of Solution Methods and their Medical Applications.","authors":"Rafaqat Khan, Amina Qureshi, Muhammad Azhar, Zia Ul Hassan, Sagheer Gul, Saeed Ahmad","doi":"10.1007/s10895-024-03809-3","DOIUrl":"10.1007/s10895-024-03809-3","url":null,"abstract":"<p><p>In the fields of health and biology, fluorescent nanomaterials have emerged as highly potential and very useful candidates for use in biosensor applications. These typical highly powerful nanomaterials are carbon dots (CDs) and graphene quantum dots (GQDs) among many other metallic nanomaterials. In the context of medical biosensors, this review article investigates the techniques of synthesis, and many uses of these nanomaterials, the obstacles that they face, and the potential for their future. We cover the significance of fluorescent nanomaterials, their use in the medical field, as well as the several techniques of synthesis for CDs and GQDs, including ultrasonication, hydrothermal, electrochemical method, surface modification, and solvothermal. In addition, we also discuss their biomedical applications, which include biomolecule detection, disease diagnosis and examine the obstacles and prospective possibilities for development of ultra-bright, ultra-sensitive, and selective biosensors for use in in-vivo research.Fluorescent carbon dots and graphene quantum dots is synthesized by using several types of raw material and methods. These Carbon dots and graphene quantum dots are used in the medical field includes detection of biomaterials, detection of cancer, virus and mutation in DNA.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"2623-2640"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent Advances in Organic Small-Molecule Fluorescent Probes for the Detection of Zinc Ions (Zn2+). 用于检测锌离子 (Zn2+) 的有机小分子荧光探针的最新进展。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-13 DOI: 10.1007/s10895-024-03770-1
Junjie Yang, Liting Tang, Lin Li, Xiongzhi Wu, Liqiang Yan
{"title":"Recent Advances in Organic Small-Molecule Fluorescent Probes for the Detection of Zinc Ions (Zn<sup>2+</sup>).","authors":"Junjie Yang, Liting Tang, Lin Li, Xiongzhi Wu, Liqiang Yan","doi":"10.1007/s10895-024-03770-1","DOIUrl":"10.1007/s10895-024-03770-1","url":null,"abstract":"<p><p>Zinc(II) ions (Zn<sup>2g</sup>) play crucial roles in the growth, propagation, and metabolism of animals, plants, and humans. Abnormal concentrations of Zn<sup>2+</sup> in the environment and living organisms pose potential risks to environmental protection and human health. Therefore, it is imperative to develop rapid, reliable and in-situ detection methods for Zn<sup>2+ </sup>in both environmental and biological contexts. Furthermore, effective analytical methods are required for diagnosing diseases and understanding physiological metabolic mechanisms associated with Zn<sup>2+</sup> concentration levels. Organic small-molecule fluorescent probes offer advantages such as fast, reliable, convenient, non-destructive detection capabilities and have significant application potential in Zn<sup>2+</sup> detection and bioimaging; thus garnering extensive attention. Over the past two years alone, various organic small-molecule probes for Zn<sup>2+</sup> based on different detection mechanisms and fluorophores have been rapidly developed. However, these probes still exhibit several limitations that need further resolution. In light of this context, we provide a comprehensive summary of the detection mechanisms, performance characteristics, and application scope of Zn<sup>2+</sup> fluorescence probes since year 2022 while highlighting their advantages. We also propose solutions to address existing issues with these probes and outline future directions for their advancement. This review aims to serve as a valuable reference source offering insights into the development of advanced organic small-molecule-based fluorescence probes specifically designed for detecting Zn<sup>2+</sup>.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"2641-2674"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel Iminocoumarin-substituted Tetraphenylethylene-based Near-infrared Fluorescent Probe for Ratiometric Detection of F- and H2S. 新颖的氨基香豆素取代四苯基乙烯基近红外荧光探针,用于按比例检测 F- 和 H2S。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-14 DOI: 10.1007/s10895-024-03801-x
Yufeng Liu, Jianing Yang, Hongliang Liu, Zhao Chen, Gang Liu, Shouzhi Pu
{"title":"Novel Iminocoumarin-substituted Tetraphenylethylene-based Near-infrared Fluorescent Probe for Ratiometric Detection of F<sup>-</sup> and H<sub>2</sub>S.","authors":"Yufeng Liu, Jianing Yang, Hongliang Liu, Zhao Chen, Gang Liu, Shouzhi Pu","doi":"10.1007/s10895-024-03801-x","DOIUrl":"10.1007/s10895-024-03801-x","url":null,"abstract":"<p><p>An iminocoumarin and tetraphenylethylene compound that exhibits aggregation-induced emission (AIE) and a significant Stokes shift (Δλ = 135 nm) in THF was created via the Knoevenagel condensation method. TPICBT could also be used as a ratiometric near-infrared fluorescent probe for the naked color identification of F<sup>-</sup> and H<sub>2</sub>S. It showed a large red shift (˃ 90 nm), good selectivity, and anti-interference. Test strip detection and cell imaging had both been accomplished using the probe. In addition, the probe could conveniently detect H<sub>2</sub>S produced during food spoilage without laboratory instruments.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3627-3637"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141317415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Tyrosinase Activity and Inhibitor Validation Based on N-GQDs Fluorescence Sensor. 基于 N-GQDs 荧光传感器的酪氨酸酶活性检测与抑制剂验证
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-14 DOI: 10.1007/s10895-024-03788-5
Jiaxin Li, Hui Guo, Weiwei Ji, Hanqi Chen, Fengju Zhao, Wei Yang, Lili Guo, Junqing Qian
{"title":"Detection of Tyrosinase Activity and Inhibitor Validation Based on N-GQDs Fluorescence Sensor.","authors":"Jiaxin Li, Hui Guo, Weiwei Ji, Hanqi Chen, Fengju Zhao, Wei Yang, Lili Guo, Junqing Qian","doi":"10.1007/s10895-024-03788-5","DOIUrl":"10.1007/s10895-024-03788-5","url":null,"abstract":"<p><p>Tyrosinase inhibitors have the ability to resist melanin formation and can be used for clinical and cosmetic, so it is becoming extremely crucial to search a rapid and effective method for detecting t the activity of tyrosinase. In this study, a sensing probe based on Nitrogen-doped graphene quantum dots (N-GQDs) were prepared with carbamide and citric acid. Tyrosinase can oxidize dopamine to dopamine quinone, which can quench the fluorescence of N-GQDs based on the principle of fluorescence resonance energy transfer (FRET) process, and then the detection of tyrosinase activity can be achieved. The result demonstrated that the fluorescence intensity of N-GQDs was a linear correlation with the activity of tyrosinase. Wide detection linear ranges between 0.05 and 5 U/mL and high selectivity. The detection range of tyrosinase was 0.05 to 5 U/mL and LOD of 0.005 U/mL. According to the above, the fluorescence method established in this work could be successfully used for the trace analysis of tyrosinase and it was verified that KA is an inhibitor of tyrosinase.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3615-3626"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141317413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experimental and Theoretical Rotational Diffusion Studies of 7DM4M1M1,8, N-2(1H)-one and 7A4T2H1B-2-one in Series of Alcohol Solvents: Stoke's-Einstein-Debye and Alavi-Waldeck Models. 7DM4M1M1,8, N-2(1H)-one 和 7A4T2H1B-2-one 在一系列酒精溶剂中的实验和理论旋转扩散研究:斯托克-爱因斯坦-德贝模型和阿拉维-瓦尔德克模型。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-04-30 DOI: 10.1007/s10895-024-03707-8
Anil Kumar, C G Renuka
{"title":"Experimental and Theoretical Rotational Diffusion Studies of 7DM4M1M1,8, N-2(1H)-one and 7A4T2H1B-2-one in Series of Alcohol Solvents: Stoke's-Einstein-Debye and Alavi-Waldeck Models.","authors":"Anil Kumar, C G Renuka","doi":"10.1007/s10895-024-03707-8","DOIUrl":"10.1007/s10895-024-03707-8","url":null,"abstract":"<p><p>Rotational diffusion studies of two solutes 7-(dimethylamino)-4-methoxy-1-methyl-1,8-naphthyridin-2(1H)-one (7DM4M1M1,8, N-2(1H)-one) and 7-amino-4-(trifluoromethyl)-2H-1-benzopyran-2-one (7A4T2H1B-2-one) having equal volumes but different chemical natures are studied in series of alcohol solvents at 303 K using steady-state methods. HOMO-LUMO, Electron density, Molecular electrostatic potential (MEP), etc., are obtained from computational calculations using Gaussian 09 software. Rotational reorientation times of 7DM4M1M1,8, N-2(1H)-one solute molecule is found to be less than 7A4T2H1B-2-one solute molecule indicates it rotates slowly in chosen solvents. Stoke's-Einstein-Debye (SED) model with stick boundary conditions for the 7A4T2H1B-2-one solute molecule is modeled to describe mechanical friction. Polar solutes along with mechanical friction also experience dielectric friction. Both these frictions being non-separable, the Alavi-Waldeck (AW) model is studied for dielectric friction contribution to the total friction solute experiences in solvents. AW model effectively explains the observed dielectric friction in alcohol solvents.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"2963-2978"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140858294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fabrication of Carbon Dots with Singlet Oxygen Generation and Their Potential Photodynamic Therapy Applications. 具有单线态氧生成功能的碳点的制作及其潜在的光动力疗法应用。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-05-08 DOI: 10.1007/s10895-024-03725-6
Pengli Zuo, Jun Peng, Yuyang Yao, Wei Tan, Lan Cheng, Jinyu Zhang, Xiuhua Lu
{"title":"Fabrication of Carbon Dots with Singlet Oxygen Generation and Their Potential Photodynamic Therapy Applications.","authors":"Pengli Zuo, Jun Peng, Yuyang Yao, Wei Tan, Lan Cheng, Jinyu Zhang, Xiuhua Lu","doi":"10.1007/s10895-024-03725-6","DOIUrl":"10.1007/s10895-024-03725-6","url":null,"abstract":"<p><p>Due to the unique chemical and biomedical properties of carbon dots (CDs), they have increasingly obtained the attention in many research fields, for example, bioimaging, fluorescence sensing, and drug delivery, etc. Recently, it was found that, under light excitation, CDs can also be exploited as a novel photosensitizer to prepare reactive oxygen species (ROS), which expand their applications in the field of photodynamic therapy for cancer treatment. Nevertheless, the high cost and complex fabrication approach of CDs significantly limit their applications. To address this issue, bottom-up routes usually utilize sustainable and inexpensive carbon precursor as starting materials, employed N,N-dimethylformamide (DMF) or ethanol as an environmental-friendly solvent. Bottom-up approach was energy efficient, and the purification process was relatively simple by dialysis. Therefore, carbon dots (CDs) were facilely fabricated in a one-pot solvothermal process using 1-aminoanthraquinone as a precursor, and their application as photosensitizers for in vitro antitumor cells, especially photodynamic therapy (PDT) was established. Then the photophysical and nanoscale dimensions properties of the fabricated CDs were characterized via TEM, UV-visible, fluorescence, and FT-IR spectroscopy. The synthesized N-doped CDs can easily dissolve in water, possess very low biotoxicity, yellow-light emission (maximum peak at 587 nm). More importantly, PDT studies demonstrated that the obtained CDs possess a high singlet oxygen yield of 35%, and exhibit significant phototoxicity to cancer cells upon 635 nm laser irradiation. These studies highlight that N-doped CDs can be facilely synthesized from only one precursor, and are a potentially novel theranostic agent for in vivo PDT.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3061-3069"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140876579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thiourea Functionalised Receptor for Selective Detection of Mercury Ions and its Application in Serum Sample. 用于选择性检测汞离子的硫脲功能化受体及其在血清样品中的应用
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-05-13 DOI: 10.1007/s10895-024-03740-7
Patitapaban Mohanty, Pragyan Parimita Dash, Swagatika Mishra, Renjith Bhaskaran, Bigyan Ranjan Jali
{"title":"Thiourea Functionalised Receptor for Selective Detection of Mercury Ions and its Application in Serum Sample.","authors":"Patitapaban Mohanty, Pragyan Parimita Dash, Swagatika Mishra, Renjith Bhaskaran, Bigyan Ranjan Jali","doi":"10.1007/s10895-024-03740-7","DOIUrl":"10.1007/s10895-024-03740-7","url":null,"abstract":"<p><p>A thiourea functionalised fluorescent probe 1-phenyl-3-(pyridin-4-yl)thiourea was synthesized and utilised as a fluorescent turn-on chemosensor for the selective recognition of Hg<sup>2+</sup> ion over competitive metal ions including Na<sup>+</sup>, Mn<sup>2+</sup>, Li<sup>+</sup>, Cr<sup>2+</sup>, Ni<sup>2+</sup>, Ca<sup>2+</sup>, Cd<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, Zn<sup>2+</sup>, Al<sup>3+</sup> and Fe<sup>2+</sup> ions based on the inter-molecular charge transfer (ICT). Intriguingly, the receptor demonstrated unique sensing capabilities for Hg<sup>2+</sup> in DMSO: H<sub>2</sub>O (10:90, v/v). The addition of Hg<sup>2+</sup> ions to the sensor resulted in a blue shift in the absorption intensity and also enhancement in fluorescence intensity at 435 nm. Fluorescence emission intensity increased linearly with Hg<sup>2+</sup> concentration ranging from 0 to 80 µL. The detection limit and binding constant were determined as 0.134 × 10<sup>-6</sup> M and 1.733 × 10<sup>7</sup> M<sup>-1</sup>, respectively. The sensing behavior of Hg<sup>2+</sup> was further examined using DLS, SEM and FTIR. The probe could detect Hg<sup>2+</sup> ions across a wide pH range. Furthermore, the receptor L demonstrated good sensing performance for Hg<sup>2+</sup> in bovine serum albumin and actual water samples.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3181-3194"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anthocyanins of Delonix Regia Floral Petals: A Novel Approach on Fluorescence Enhancement, Forster Resonance Energy Transfer Mechanism and Photostability Studies for Optoelectronic Applications. Delonix Regia 花瓣的花青素:光电应用中的荧光增强、福斯特共振能量转移机制和光稳定性研究新方法。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-05-13 DOI: 10.1007/s10895-024-03730-9
D Harshitha, Anil Kumar, H M Mahesh, C G Renuka
{"title":"Anthocyanins of Delonix Regia Floral Petals: A Novel Approach on Fluorescence Enhancement, Forster Resonance Energy Transfer Mechanism and Photostability Studies for Optoelectronic Applications.","authors":"D Harshitha, Anil Kumar, H M Mahesh, C G Renuka","doi":"10.1007/s10895-024-03730-9","DOIUrl":"10.1007/s10895-024-03730-9","url":null,"abstract":"<p><p>In this work, we focused on extracting the anthocyanin dye in acetone, butanol, ethanol, and water solvents from Delonix regia flowers by a simple maceration extraction process. The identification of functional group analysis, vibrational studies, energy transfer mechanisms, optoelectronic properties, photostability studies, FRET-assisted potential light emissions and photometric properties of the anthocyanin dyes are successively investigated. FTIR spectroscopy and vibrational studies have confirmed the existence of polyphenolic groups in 2-phenyl chromenylium (anthocyanin) dyes. The optoelectronic results show the least direct bandgap (2.04 eV), indirect bandgap (1.55 eV), Urbach energy (0.380 eV), high refractive index (1.20), dielectric constant (2.794), and high optical conductivity (1.954 × 10<sup>3</sup> S/m) for the anthocyanin dye extracted found in water solvent. The photoluminescence properties such as Stoke's shift, high quantum yield, and lifetime results show that anthocyanin dyes are promising candidates for red-LEDs and optical materials. The absorption and emission spectra of the anthocyanin dyes follow the mirror image rule and the Franck-Condon factor exists between vibrational energy levels corresponding to all the electronic transitions. The excellent correspondence between the absorption and emission spectra reinforces that the anthocyanins are efficient (46%) FRET probes. Further, photometric properties such as CIE, CRI, CCT and colour purity results of anthocyanins in all studied solvents revealed that this material exhibits orange to red shades (x = 0.48 → 0.54 and y = 0.36 →0.45) and is well suitable for have great potential in the manufacturing of Organic-LEDs and other optoelectronic device applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3195-3226"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Citrus Medica-derived Fluorescent Carbon Dots for the Imaging of Vigna Radiate Root Cells. 源于柑橘类植物的荧光碳点用于成像木槿根细胞。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-10 DOI: 10.1007/s10895-024-03790-x
Meera Varghese, Yatheesharadhya Bylappa, Anish Nag, Partha Kumbhakar, Manoj Balachandran
{"title":"Citrus Medica-derived Fluorescent Carbon Dots for the Imaging of Vigna Radiate Root Cells.","authors":"Meera Varghese, Yatheesharadhya Bylappa, Anish Nag, Partha Kumbhakar, Manoj Balachandran","doi":"10.1007/s10895-024-03790-x","DOIUrl":"10.1007/s10895-024-03790-x","url":null,"abstract":"<p><p>Bio-imaging is a crucial tool for researchers in the fields of cell biology and developmental biomedical sector. Among the various available imaging techniques, fluorescence based imaging stands out due to its high sensitivity and specificity. However, traditional fluorescent materials used in biological imaging often suffer from issues such as photostability and biocompatibility. Moreover, plant tissues contain compounds that cause autofluorescence and light scattering, which can hinder fluorescence microscopy effectiveness. This study explores the development of fluorescent carbon dots (Cm-CDs) synthesized from Citrus medica fruit extract for the fluorescence imaging of Vigna radiata root cells. The successful synthesis of CDs with an average size of 6.7 nm is confirmed by Transmission Electron Microscopy (TEM). The X-ray diffraction (XRD) analysis and raman spectroscopy indicated that the obtained CDs are amorphous in nature. The presence of various functional groups on the surface of CDs were identified by Fourier transform infrared (FTIR) spectra. The optical characteristics of Cm-CDs were studied by UV-Visible spectroscopy and photoluminescence spectroscopy. Cm-CDs demonstrated strong excitation-dependent fluorescence, good solubility, and effective penetration in to the Vigna radiata root cells with multicolor luminescence, and addressed autofluorescence issues. Additionally, a comparative analysis determined the optimal concentration for high-resolution, multi-color root cell imaging, with Cm-CD2 (2.5 mg/ml) exhibiting the highest photoluminescence (PL) intensity. These findings highlight the potential of Cm-CDs in enhancing direct endocytosis and overcoming autofluorescence in plant cell imaging, offering promising advancements for cell biology research.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3519-3527"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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