Journal of Fluorescence最新文献

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L-Arginine Doped Carbon Nanodots from Cinnamon Bark for Improved Fluorescent Yeast Cell Imaging. 肉桂皮中掺入 L-精氨酸的碳纳米点用于改进酵母细胞荧光成像。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-13 DOI: 10.1007/s10895-024-03799-2
Urvi M Lad, Dhruti J Dave, Bhumi N Desai, Devesh H Suthar, Chetan K Modi
{"title":"L-Arginine Doped Carbon Nanodots from Cinnamon Bark for Improved Fluorescent Yeast Cell Imaging.","authors":"Urvi M Lad, Dhruti J Dave, Bhumi N Desai, Devesh H Suthar, Chetan K Modi","doi":"10.1007/s10895-024-03799-2","DOIUrl":"10.1007/s10895-024-03799-2","url":null,"abstract":"<p><p>In this study, we present an economical and efficient synthesis method for carbon nanodots (CNDs) derived from cinnamon bark wood powder, with the incorporation of L-arginine as a dopant at varying ratios (Cinnamon : L-Arginine - 1:0.25, 1:0.5) via a hydrothermal reaction. Extensive structural and optical characterization was conducted through techniques such as FTIR, XRD, HR-TEM, DLS, UV-Vis, and PL spectra, providing a comprehensive understanding of the properties of CNDs and doped-CNDs. Quantum yields (QY) were quantified for synthesized materials, contributing to the assessment of their fluorescence efficiency. The synthesized CNDs were successfully applied for bioimaging of yeast cells, employing fluorescence microscopy to visualize their interaction. Remarkably, L-arginine-doped CNDs exhibited enhanced fluorescence, showcasing the influence of the dopant. The nature of these CNDs was rigorously investigated, confirming their biocompatibility. Notably, this work presents a novel approach to synthesizing CNDs from a renewable and sustainable source, cinnamon bark wood powder, while exploring the effects of L-arginine doping on their optical and biological properties. This work not only contributes to the synthesis and characterization of CNDs but also highlights their potential for diverse applications, emphasizing their structural, optical, and biological attributes. The findings underscore the versatility of CNDs derived from cinnamon bark wood powder and their potential for advancing biotechnological and imaging applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3577-3587"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Excitation wavelength and time dependent colour tunable room temperature phosphorescence from boron doped carbon nanodots. 更正:掺硼碳纳米点的激发波长和时间相关性彩色可调室温磷光。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 DOI: 10.1007/s10895-024-04039-3
Bilipang Mahilary, Khemnath Patir, Sanjay Basumatary
{"title":"Correction: Excitation wavelength and time dependent colour tunable room temperature phosphorescence from boron doped carbon nanodots.","authors":"Bilipang Mahilary, Khemnath Patir, Sanjay Basumatary","doi":"10.1007/s10895-024-04039-3","DOIUrl":"10.1007/s10895-024-04039-3","url":null,"abstract":"","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3685"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green Synthesis of CQDs via the Trunks of Bauhinia purpurea as Fluorescence Probes for Rapid and Accurate Detection of Quinoline Yellow. 通过紫荆树干绿色合成 CQDs 作为荧光探针,用于快速准确地检测喹啉黄。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-05-02 DOI: 10.1007/s10895-024-03699-5
Ziyang Ge, Haiying Du, Bo Tang, Jie Deng
{"title":"Green Synthesis of CQDs via the Trunks of Bauhinia purpurea as Fluorescence Probes for Rapid and Accurate Detection of Quinoline Yellow.","authors":"Ziyang Ge, Haiying Du, Bo Tang, Jie Deng","doi":"10.1007/s10895-024-03699-5","DOIUrl":"10.1007/s10895-024-03699-5","url":null,"abstract":"<p><p>Carbon quantum dots (CQDs) were greenly synthesized via a single-step hydrothermal method, using the trunks of Bauhinia purpurea as the carbon source. They exhibited good dispersibility, water solubility, high sensitivity, and great stability with a spherical form and a particle size of 2.68 ± 0.32 nm. By utilizing the inner filter effect and dynamic quenching effect, the fluorescence quenching of CQDs can be induced to detect quinoline yellow. Detailed experimental results showed that the change rate of fluorescence intensity of CQDs had a good linear relationship with varying concentrations of quinoline yellow (2-128 µmol/L). It can be clearly observed that the fluorescence quenching occurred within 1 min, its correlation coefficient (R<sup>2</sup>) is 0.9912, and the detection limit (DL) is 1.7884 μmol/L, substantially lower than the maximum concentration stipulated by the national standard of 209.5 µmol/L. Furthermore, quinoline yellow had been successfully detected in real beverage samples using CQDs, with the recovery rates of 90.6%-110.4% and the relative standard deviation (RSD) ≤ 6.3% and it also showed great anti-interference and selectivity. These findings indicate that the detected quinoline yellow of CQDs possess substantial promise for a wide range of applications within the detected artificial food colors field.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3019-3032"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140859922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Imidazo[1, 2-a]Pyridine Based Fluorescent Probe for the Continuous "on-off-on" Detection of Fe3+ and F. 一种基于咪唑并[1, 2-a]吡啶的荧光探针,用于 "开-关-开 "连续检测 Fe3+ 和 F。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-05-16 DOI: 10.1007/s10895-024-03763-0
Lei Xue, Yelin Yao, Xin Wang, Jichen Yin, Haibin Wang
{"title":"An Imidazo[1, 2-a]Pyridine Based Fluorescent Probe for the Continuous \"on-off-on\" Detection of Fe<sup>3+</sup> and F<sup />.","authors":"Lei Xue, Yelin Yao, Xin Wang, Jichen Yin, Haibin Wang","doi":"10.1007/s10895-024-03763-0","DOIUrl":"10.1007/s10895-024-03763-0","url":null,"abstract":"<p><p>An \"on-off-on\" fluorescent probe LK was synthesized from 2-benzoylpyridine and o-vanillin, which could sequentially detect Fe<sup>3+</sup> and F<sup>-</sup> in DMSO/H<sub>2</sub>O solutions (v/v = 1/1, HEPES buffer, 1.0 mM, pH 7.0) with large Stokes shift (178 nm). LK exhibited not only high selectivity and sensitivity towards Fe<sup>3+</sup> and F<sup>-</sup>, but also strong anti-interference ability to other ions. LK was coordinated with Fe<sup>3+</sup> at a ratio of 2:1, with a binding constant (K<sub>a</sub>) of 1.3 × 10<sup>4</sup> M<sup>- 1</sup>. The detection limits for Fe<sup>3+</sup> and F<sup>-</sup> were 6.9 × 10<sup>- 8</sup> M and 3.0 × 10<sup>- 7</sup> M, respectively. Due to its excellent sensing performance, LK was successfully applied in actual water samples and test papers for the detection of Fe<sup>3+</sup> and F<sup>-</sup>.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3313-3320"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aggregation-Induced Emission (AIE) in Polymers: Effect of Polymer size on the Fluorescence of Low Molecular Weight PEG and PPG. 聚合物中的聚集诱导发射(AIE):聚合物尺寸对低分子量 PEG 和 PPG 荧光的影响。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-05-23 DOI: 10.1007/s10895-024-03776-9
Nathalia Vieira Porphirio Veríssimo, Elionio Galvão Frota, Juliana Barone Teixeira, Valéria de Carvalho Santos-Ebinuma, Ricardo Pinheiro de Souza Oliveira
{"title":"Aggregation-Induced Emission (AIE) in Polymers: Effect of Polymer size on the Fluorescence of Low Molecular Weight PEG and PPG.","authors":"Nathalia Vieira Porphirio Veríssimo, Elionio Galvão Frota, Juliana Barone Teixeira, Valéria de Carvalho Santos-Ebinuma, Ricardo Pinheiro de Souza Oliveira","doi":"10.1007/s10895-024-03776-9","DOIUrl":"10.1007/s10895-024-03776-9","url":null,"abstract":"<p><p>Aggregation-induced emission (AIE) is a fascinating phenomenon where specific molecules exhibit enhanced fluorescence upon aggregation. This unique property has revolutionized the design and development of new fluorescent materials for different applications, from biosensors and organic light-emitting diodes (OLEDs) to biomedical imaging and diagnostics. Researchers are creating sensitive and selective sensing platforms, opening new avenues in material science and engineering by harnessing the potential of AIE. To expand the knowledge in this field, this study explored the aggregation-induced emission (AIE) properties of two polymers, namely polyethylene glycol (PEG) and polypropylene glycol (PPG) of low molecular weight (MW) using fluorescence spectroscopy and absorbance (UV). PEG-300 and PPG-725 were the most fluorescent polymers at UV of the ten investigated. Interestingly, AIE did not correlate linearly with molecular weight (MW), and monobutyl ether substitution in PEG with a similar MW substantially altered its AIE. Furthermore, fluorescence precisely quantified low polymer concentrations in water, and non-aqueous solvents suppressed AIE, suggesting potential for AIE manipulation. These findings enhance our understanding of AIE in polymers, fostering the development of novel materials for applications such as biosensors.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3385-3392"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescent Nitrogen-doped Carbon Dots-based Turn-off Sensor for Bilirubin. 基于荧光掺氮碳点的胆红素关断传感器
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-12 DOI: 10.1007/s10895-024-03771-0
Aswathy A O, B A Akhila, Sony George
{"title":"Fluorescent Nitrogen-doped Carbon Dots-based Turn-off Sensor for Bilirubin.","authors":"Aswathy A O, B A Akhila, Sony George","doi":"10.1007/s10895-024-03771-0","DOIUrl":"10.1007/s10895-024-03771-0","url":null,"abstract":"<p><p>Bilirubin (BR), a heme protein produced from breakdown of haemoglobin is present in aged red blood cells; whose abnormal concentration is associated with diseases like hyperbilirubinemia, coronary disease, iron deficiency, and so on. Herein, we have synthesized a selective, sensitive, and low-cost sensing platform using fluorescent nitrogen doped carbon dots (NCDs), prepared from precursors; citric acid and urea via a simple microwave-assisted method. The emission at 444 nm on excitation with 360 nm was well quenched in presence of BR suggesting a direct turn-off detection for BR. Characterization of developed probe was done by UV-Visible absorption studies, photoluminescence studies, SEM, TEM, ATR-FTIR, XPS, and DLS analysis. BR was detected with a Limit of Detection (LoD) and Limit of Quantification (LoQ) of 0.32 µM and 1.08 µM respectively. NCDs exhibited excellent selectivity and sensitivity towards BR in the presence of co-existing biomolecules and ions. Practical feasibility was checked by paper-strip-based sensing of BR and spiked real human samples were used for conducting real sample analysis.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3565-3576"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent Progress of Fluorescent Carbon Dots and Graphene Quantum Dots for Biosensors: Synthesis of Solution Methods and their Medical Applications. 用于生物传感器的荧光碳点和石墨烯量子点的最新进展:溶液方法的合成及其医学应用》。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-13 DOI: 10.1007/s10895-024-03809-3
Rafaqat Khan, Amina Qureshi, Muhammad Azhar, Zia Ul Hassan, Sagheer Gul, Saeed Ahmad
{"title":"Recent Progress of Fluorescent Carbon Dots and Graphene Quantum Dots for Biosensors: Synthesis of Solution Methods and their Medical Applications.","authors":"Rafaqat Khan, Amina Qureshi, Muhammad Azhar, Zia Ul Hassan, Sagheer Gul, Saeed Ahmad","doi":"10.1007/s10895-024-03809-3","DOIUrl":"10.1007/s10895-024-03809-3","url":null,"abstract":"<p><p>In the fields of health and biology, fluorescent nanomaterials have emerged as highly potential and very useful candidates for use in biosensor applications. These typical highly powerful nanomaterials are carbon dots (CDs) and graphene quantum dots (GQDs) among many other metallic nanomaterials. In the context of medical biosensors, this review article investigates the techniques of synthesis, and many uses of these nanomaterials, the obstacles that they face, and the potential for their future. We cover the significance of fluorescent nanomaterials, their use in the medical field, as well as the several techniques of synthesis for CDs and GQDs, including ultrasonication, hydrothermal, electrochemical method, surface modification, and solvothermal. In addition, we also discuss their biomedical applications, which include biomolecule detection, disease diagnosis and examine the obstacles and prospective possibilities for development of ultra-bright, ultra-sensitive, and selective biosensors for use in in-vivo research.Fluorescent carbon dots and graphene quantum dots is synthesized by using several types of raw material and methods. These Carbon dots and graphene quantum dots are used in the medical field includes detection of biomaterials, detection of cancer, virus and mutation in DNA.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"2623-2640"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent Advances in Organic Small-Molecule Fluorescent Probes for the Detection of Zinc Ions (Zn2+). 用于检测锌离子 (Zn2+) 的有机小分子荧光探针的最新进展。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-13 DOI: 10.1007/s10895-024-03770-1
Junjie Yang, Liting Tang, Lin Li, Xiongzhi Wu, Liqiang Yan
{"title":"Recent Advances in Organic Small-Molecule Fluorescent Probes for the Detection of Zinc Ions (Zn<sup>2+</sup>).","authors":"Junjie Yang, Liting Tang, Lin Li, Xiongzhi Wu, Liqiang Yan","doi":"10.1007/s10895-024-03770-1","DOIUrl":"10.1007/s10895-024-03770-1","url":null,"abstract":"<p><p>Zinc(II) ions (Zn<sup>2g</sup>) play crucial roles in the growth, propagation, and metabolism of animals, plants, and humans. Abnormal concentrations of Zn<sup>2+</sup> in the environment and living organisms pose potential risks to environmental protection and human health. Therefore, it is imperative to develop rapid, reliable and in-situ detection methods for Zn<sup>2+ </sup>in both environmental and biological contexts. Furthermore, effective analytical methods are required for diagnosing diseases and understanding physiological metabolic mechanisms associated with Zn<sup>2+</sup> concentration levels. Organic small-molecule fluorescent probes offer advantages such as fast, reliable, convenient, non-destructive detection capabilities and have significant application potential in Zn<sup>2+</sup> detection and bioimaging; thus garnering extensive attention. Over the past two years alone, various organic small-molecule probes for Zn<sup>2+</sup> based on different detection mechanisms and fluorophores have been rapidly developed. However, these probes still exhibit several limitations that need further resolution. In light of this context, we provide a comprehensive summary of the detection mechanisms, performance characteristics, and application scope of Zn<sup>2+</sup> fluorescence probes since year 2022 while highlighting their advantages. We also propose solutions to address existing issues with these probes and outline future directions for their advancement. This review aims to serve as a valuable reference source offering insights into the development of advanced organic small-molecule-based fluorescence probes specifically designed for detecting Zn<sup>2+</sup>.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"2641-2674"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel Iminocoumarin-substituted Tetraphenylethylene-based Near-infrared Fluorescent Probe for Ratiometric Detection of F- and H2S. 新颖的氨基香豆素取代四苯基乙烯基近红外荧光探针,用于按比例检测 F- 和 H2S。
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-14 DOI: 10.1007/s10895-024-03801-x
Yufeng Liu, Jianing Yang, Hongliang Liu, Zhao Chen, Gang Liu, Shouzhi Pu
{"title":"Novel Iminocoumarin-substituted Tetraphenylethylene-based Near-infrared Fluorescent Probe for Ratiometric Detection of F<sup>-</sup> and H<sub>2</sub>S.","authors":"Yufeng Liu, Jianing Yang, Hongliang Liu, Zhao Chen, Gang Liu, Shouzhi Pu","doi":"10.1007/s10895-024-03801-x","DOIUrl":"10.1007/s10895-024-03801-x","url":null,"abstract":"<p><p>An iminocoumarin and tetraphenylethylene compound that exhibits aggregation-induced emission (AIE) and a significant Stokes shift (Δλ = 135 nm) in THF was created via the Knoevenagel condensation method. TPICBT could also be used as a ratiometric near-infrared fluorescent probe for the naked color identification of F<sup>-</sup> and H<sub>2</sub>S. It showed a large red shift (˃ 90 nm), good selectivity, and anti-interference. Test strip detection and cell imaging had both been accomplished using the probe. In addition, the probe could conveniently detect H<sub>2</sub>S produced during food spoilage without laboratory instruments.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3627-3637"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141317415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Tyrosinase Activity and Inhibitor Validation Based on N-GQDs Fluorescence Sensor. 基于 N-GQDs 荧光传感器的酪氨酸酶活性检测与抑制剂验证
IF 2.6 4区 化学
Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-06-14 DOI: 10.1007/s10895-024-03788-5
Jiaxin Li, Hui Guo, Weiwei Ji, Hanqi Chen, Fengju Zhao, Wei Yang, Lili Guo, Junqing Qian
{"title":"Detection of Tyrosinase Activity and Inhibitor Validation Based on N-GQDs Fluorescence Sensor.","authors":"Jiaxin Li, Hui Guo, Weiwei Ji, Hanqi Chen, Fengju Zhao, Wei Yang, Lili Guo, Junqing Qian","doi":"10.1007/s10895-024-03788-5","DOIUrl":"10.1007/s10895-024-03788-5","url":null,"abstract":"<p><p>Tyrosinase inhibitors have the ability to resist melanin formation and can be used for clinical and cosmetic, so it is becoming extremely crucial to search a rapid and effective method for detecting t the activity of tyrosinase. In this study, a sensing probe based on Nitrogen-doped graphene quantum dots (N-GQDs) were prepared with carbamide and citric acid. Tyrosinase can oxidize dopamine to dopamine quinone, which can quench the fluorescence of N-GQDs based on the principle of fluorescence resonance energy transfer (FRET) process, and then the detection of tyrosinase activity can be achieved. The result demonstrated that the fluorescence intensity of N-GQDs was a linear correlation with the activity of tyrosinase. Wide detection linear ranges between 0.05 and 5 U/mL and high selectivity. The detection range of tyrosinase was 0.05 to 5 U/mL and LOD of 0.005 U/mL. According to the above, the fluorescence method established in this work could be successfully used for the trace analysis of tyrosinase and it was verified that KA is an inhibitor of tyrosinase.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"3615-3626"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141317413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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