Journal of Eukaryotic Microbiology最新文献_第4页

How to overcome constraints imposed by microsporidian genome features to ensure gene prediction? 如何克服微孢子虫基因组特征带来的限制以确保基因预测？

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-06-27 DOI: 10.1111/jeu.13038

Eric Peyretaillade, Reginal F. Akossi, Jérémy Tournayre, Frédéric Delbac, Ivan Wawrzyniak

{"title":"How to overcome constraints imposed by microsporidian genome features to ensure gene prediction?","authors":"Eric Peyretaillade, Reginal F. Akossi, Jérémy Tournayre, Frédéric Delbac, Ivan Wawrzyniak","doi":"10.1111/jeu.13038","DOIUrl":"10.1111/jeu.13038","url":null,"abstract":"Since the advent of sequencing techniques and due to their continuous evolution, it has become easier and less expensive to obtain the complete genome sequence of any organism. Nevertheless, to elucidate all biological processes governing organism development, quality annotation is essential. In genome annotation, predicting gene structure is one of the most important and captivating challenges for computational biology. This aspect of annotation requires continual optimization, particularly for genomes as unusual as those of microsporidia. Indeed, this group of fungal-related parasites exhibits specific features (highly reduced gene sizes, sequences with high rate of evolution) linked to their evolution as intracellular parasites, requiring the implementation of specific annotation approaches to consider all these features. This review aimed to outline these characteristics and to assess the increasingly efficient approaches and tools that have enhanced the accuracy of gene prediction for microsporidia, both in terms of sensitivity and specificity. Subsequently, a final part will be dedicated to postgenomic approaches aimed at reinforcing the annotation data generated by prediction software. These approaches include the characterization of other understudied genes, such as those encoding regulatory noncoding RNAs or very small proteins, which also play crucial roles in the life cycle of these microorganisms.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Updated classification of the phylum Parabasalia 更新了寄生虫门的分类。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-06-02 DOI: 10.1111/jeu.13035

Vittorio Boscaro, Erick R. James, Rebecca Fiorito, Javier del Campo, Rudolf H. Scheffrahn, Patrick J. Keeling

{"title":"Updated classification of the phylum Parabasalia","authors":"Vittorio Boscaro, Erick R. James, Rebecca Fiorito, Javier del Campo, Rudolf H. Scheffrahn, Patrick J. Keeling","doi":"10.1111/jeu.13035","DOIUrl":"10.1111/jeu.13035","url":null,"abstract":"The phylum Parabasalia includes very diverse single-cell organisms that nevertheless share a distinctive set of morphological traits. Most are harmless or beneficial gut symbionts of animals, but some have turned into parasites in other body compartments, the most notorious example being Trichomonas vaginalis in humans. Parabasalians have garnered attention for their nutritional symbioses with termites, their modified anaerobic mitochondria (hydrogenosomes), their character evolution, and the wholly unique features of some species. The molecular revolution confirmed the monophyly of Parabasalia, but considerably changed our view of their internal relationships, prompting a comprehensive reclassification 14 years ago. This classification has remained authoritative for many subgroups despite a greatly expanded pool of available data, but the large number of species and sequences that have since come out allow for taxonomic refinements in certain lineages, which we undertake here. We aimed to introduce as little disruption as possible but at the same time ensure that most taxa are truly monophyletic, and that the larger clades are subdivided into meaningful units. In doing so, we also highlighted correlations between the phylogeny of parabasalians and that of their hosts.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141198981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bit by bit toward the diversity of metopids: Description of the genus Pidimetopus n. gen. (Ciliophora: Armophorea) 逐步实现栉水母的多样性：描述 Pidimetopus n. gen.属（纤毛虫：腕足动物）。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-06-01 DOI: 10.1111/jeu.13034

Ondřej Pomahač, Daniel Méndez-Sánchez, Ivan Čepička

{"title":"Bit by bit toward the diversity of metopids: Description of the genus Pidimetopus n. gen. (Ciliophora: Armophorea)","authors":"Ondřej Pomahač, Daniel Méndez-Sánchez, Ivan Čepička","doi":"10.1111/jeu.13034","DOIUrl":"10.1111/jeu.13034","url":null,"abstract":"While metopids (Armophorea: Metopida) represent the most species-rich group of free-living anaerobic ciliates thriving in hypoxic environments, our understanding of their true diversity remains incomplete. Most metopid species are still characterized only morphologically. Particularly, the so-called IAC clade (named in the past after some of the taxa included, Idiometopus, Atopospira, and Clevelandellida), comprising free-living members as well as the endosymbiotic ones (order Clevelandellida), is in serious need of revision. In our study, we establish a new free-living genus in the IAC clade, Pidimetopus n. gen., with descriptions of two new species, P. nanus n. sp., and P. permonicus n. sp., using up-to-date molecular and morphologic methods. The genus is characterized by small cells (up to 75 μm long), not more than 10 adoral membranelles and eight somatic kineties, and usually, four long caudal cilia that can stiffen. In addition to morphologic and molecular characterizations, we also conducted a statistical morphotype analysis of the polymorphic species P. nanus n. sp. We discuss the relevance of the earlier morphologically described species Metopus minor as a putative collective taxon for several small metopids less than 50 μm long.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome evolution in intracellular parasites: Microsporidia and Apicomplexa 细胞内寄生虫的基因组进化：微孢子虫和吸虫。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-05-24 DOI: 10.1111/jeu.13033

Amjad Khalaf, Ore Francis, Mark L. Blaxter

引用次数: 0

Anoctamin-like protein 1 regulates repolarization in Paramecium behavioral responses 类鼻氨蛋白 1 调节鹦鹉螺行为反应中的再极化。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-05-17 DOI: 10.1111/jeu.13030

Kana Sakoshita, Shotaro Aratani, Nana Kameda, Ryo Takebe, Takashi Tominaga, Masaki Ishida, Manabu Hori

{"title":"Anoctamin-like protein 1 regulates repolarization in Paramecium behavioral responses","authors":"Kana Sakoshita, Shotaro Aratani, Nana Kameda, Ryo Takebe, Takashi Tominaga, Masaki Ishida, Manabu Hori","doi":"10.1111/jeu.13030","DOIUrl":"10.1111/jeu.13030","url":null,"abstract":"Paramecium exhibits responsive behavior to environmental changes, moving either closer to or further away from stimuli. Electrophysiological experiments have revealed that these behavioral responses are controlled by membrane potentials. Anoctamin, a Ca2+-activated Cl− channel, is involved in the regulation of membrane potential in mammals. However, it remains uncertain whether Cl− channels like anoctamin regulate Paramecium behavior. Herein, replacement of external Cl− ions with acetate ion and application of Cl− channel blocker niflumic acid (NFA, 0.1 μM) increased spontaneous avoiding reactions (sARs). Hence, we hypothesized that anoctamin is involved in the stabilization of membrane potential fluctuation. Paramecium cells in which the anoctamin-like protein 1 gene was knocked down displayed frequent sARs in the culture medium without external stimulation. Treatment of anoctamin-like protein 1-knockdown cells with the Ca2+ chelator BAPTA or Ca-channel blocker nicardipine reversed the increase in sARs. Electrophysiological experiments revealed extension of membrane depolarization when positive currents were applied to anoctamin-like protein 1-knockdown cells. We concluded that anoctamin-like protein 1 works as a Cl-channel and stabilizes the membrane potential oscillation, reducing sARs.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Naegleria lustrarea n. sp. (Excavata, Discoba, Heterolobosea) from the feces of Ambystoma annulatum (Ringed Salamander) in Northwest Arkansas 从阿肯色州西北部的环纹蝾螈粪便中分离出 Naegleria lustrarea n. sp.（Excavata、Discoba、Heterolobosea）。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-05-09 DOI: 10.1111/jeu.13031

Brian M. Becker, Idan Banson, James M. Walker, Anant Deshwal, Matthew W. Brown, Jeffery D. Silberman

引用次数: 0

Microsporidia: Pervasive natural pathogens of Caenorhabditis elegans and related nematodes 小孢子虫：秀丽隐杆线虫和相关线虫的普遍自然病原体

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-05-03 DOI: 10.1111/jeu.13027

Spencer S. Gang, Vladimir Lažetić

引用次数: 0

Redescription and molecular characterization of Loxocephalus luridus Eberhard, 1862 based on Czech populations: Implications for order Loxocephalida Jankowski, 1980 基于捷克种群的 Loxocephalus luridus Eberhard, 1862 的重新描述和分子特征：Jankowski, 1980

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-04-12 DOI: 10.1111/jeu.13028

William Bourland, Ondřej Pomahač, Kateřina Poláková, Ivan Čepička

{"title":"Redescription and molecular characterization of Loxocephalus luridus Eberhard, 1862 based on Czech populations: Implications for order Loxocephalida Jankowski, 1980","authors":"William Bourland, Ondřej Pomahač, Kateřina Poláková, Ivan Čepička","doi":"10.1111/jeu.13028","DOIUrl":"10.1111/jeu.13028","url":null,"abstract":"The phylogenetic and taxonomic affinities of lineages currently assigned to the non-monophyletic ciliate order Loxocephalida Jankowski (1980) within subclass Scuticociliatia Small (1967) remain unresolved. In the current study, we redescribe the morphology of the type species, Loxocephalus luridus Eberhard (1862) based on two Czech populations and include the first scanning and transmission electron microscopy images of the species. We provide the first 18S rRNA gene sequences for L. luridus and consider its phylogenetic position. Our results support the separation of Dexiotricha from Loxocephalus; however, the former genus is recovered as non-monophyletic. The monophyly of genus Dexiotricha and that of Loxocephalus + Dexiotricha is rejected. Loxocephalus luridus, together with Dexiotricha species, nests within a fully supported clade with Conchophthirus species, long presumed to belong to the Pleuronematida. Haptophrya is recovered as sister to this clade. The monophyly of the Astomatia Schewiakoff (1896) including Haptophrya is rejected. No clear morphologic synapomorphy is identified for the fully supported clade consisting of Haptophrya, Dexiotricha, Loxocephalus, and Conchophthirus.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140590141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogen- and host-directed pharmacologic strategies for control of Vairimorpha (Nosema) spp. infection in honey bees 控制蜜蜂Vairimorpha（Nosema）属感染的病原体和宿主定向药理策略

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-04-04 DOI: 10.1111/jeu.13026

Parker Parrella, Annabelle B. Elikan, Jonathan W. Snow

{"title":"Pathogen- and host-directed pharmacologic strategies for control of Vairimorpha (Nosema) spp. infection in honey bees","authors":"Parker Parrella, Annabelle B. Elikan, Jonathan W. Snow","doi":"10.1111/jeu.13026","DOIUrl":"10.1111/jeu.13026","url":null,"abstract":"Microsporidia are obligate intracellular parasites of the Fungal Kingdom that cause widespread infections in nature, with important effects on invertebrates involved in food production systems. The two microsporidian species Vairimorpha (Nosema) ceranae (and the less common Vairimorpha (Nosema) apis) can cause individual disease in honey bees and contribute to colony collapse. The efficacy, safety, and availability of fumagillin, the only drug currently approved to treat microsporidia infection in bees, is uncertain. In this review, we will discuss some of the most promising alternative strategies for the mitigation of Vairimorpha spp. with an emphasis on infection by V. ceranae, now the dominant species infecting bees. We will focus on pharmacologic interventions where the mechanism of action is known and examine both pathogen-directed and host-directed approaches. As limiting toxicity to host cells has been especially emphasized in treating bees that are already facing numerous stressors, strategies that disrupt pathogen-specific targets may be especially advantageous. Therefore, efforts to increase the knowledge and tools for facilitating the discovery of such targets and pharmacologic agents directed against them should be prioritized.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140590208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions between microsporidia and other members of the microbiome 微孢子虫与微生物组其他成员之间的相互作用。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-04-01 DOI: 10.1111/jeu.13025

Jonathan Tersigni, Hala Tamim El Jarkass, Edward B. James, Aaron W. Reinke

{"title":"Interactions between microsporidia and other members of the microbiome","authors":"Jonathan Tersigni, Hala Tamim El Jarkass, Edward B. James, Aaron W. Reinke","doi":"10.1111/jeu.13025","DOIUrl":"10.1111/jeu.13025","url":null,"abstract":"The microbiome is the collection of microbes that are associated with a host. Microsporidia are intracellular eukaryotic parasites that can infect most types of animals. In the last decade, there has been much progress to define the relationship between microsporidia and the microbiome. In this review, we cover an increasing number of reports suggesting that microsporidia are common components of the microbiome in both invertebrates and vertebrates. These microsporidia infections can range from mutualistic to pathogenic, causing several physiological phenotypes, including death. Infection with microsporidia often causes a disruption in the normal microbiome, with both increases and decreases of bacterial, fungal, viral, and protozoan species being observed. This impact on the microbiome can occur through upregulation and downregulation of innate immunity as well as morphological changes to tissues that impact interactions with these microbes. Other microbes, particularly bacteria, can inhibit microsporidia and have been exploited to control microsporidia infections. These bacteria can function through regulating immunity, secreting anti-microsporidia compounds, and, in engineered versions, expressing double-stranded RNA targeting microsporidia genes. We end this review by discussing potential future directions to further understand the complex interactions between microsporidia and the other members of the microbiome.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0