Journal of Eukaryotic Microbiology最新文献_第5页

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-14

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IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-05

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IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-05

引用次数: 0

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-05

引用次数: 0

Life Cycle Plasticity of Colpoda aspera Fed With Petroleum Tolerant Gram-Positive and Gram-Negative Bacteria. 耐油革兰氏阳性菌和革兰氏阴性菌喂喂水蛭的生命周期可塑性。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-01 DOI: 10.1111/jeu.70085

Laura Mondragón-Camarillo, Cruz Carlos Castillo Camacho, Salvador Rodríguez-Zaragoza

{"title":"Life Cycle Plasticity of Colpoda aspera Fed With Petroleum Tolerant Gram-Positive and Gram-Negative Bacteria.","authors":"Laura Mondragón-Camarillo, Cruz Carlos Castillo Camacho, Salvador Rodríguez-Zaragoza","doi":"10.1111/jeu.70085","DOIUrl":"10.1111/jeu.70085","url":null,"abstract":"Soil recovery after contamination relies on the surviving microbiota to reconstruct microbial food webs. The ciliate Colpoda aspera and Brevundimonas sp., Rhizobium sp1, Rhizobium sp2 (gram-negative), Bacillus sp1, Bacillus sp2, and Microbacterium sp.(gram-positive) remain active after pulses of light petroleum contamination. We used phase contrast microscopy to assess excystation time and cyst production by C. aspera after placing 65 cysts in petri dishes with 0.5% LNA medium and adding 100 μL of a 5.2 × 108 cell bacterial inoculum from contaminated soil. C. aspera excystation was monitored by triplicate every hour for 72 h. Excystation occurred faster and resulted in a higher number of cysts when fed with gram-negative bacteria, particularly Rhizobium sp2 and Brevundimonas sp. In contrast, C. aspera cysts formed only two daughter cells when fed with Microbacterium sp., reducing the quantity and speed of reproduction. This reproductive behavior may allow other predatory ciliates to coexist when Microbacterium sp. becomes prevalent in a soil hotspot. Ciliates' feeding preferences and nutritional value are required to understand resource partitioning and understanding these interactions can inform strategies to promote resilient microbial communities and accelerate recovery after contamination.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 3","pages":"e70085"},"PeriodicalIF":3.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13140017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A New Look at the Phylogenetic Relationships Within Class Karyorelictea. 对核生物纲系统发育关系的新认识。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-01 DOI: 10.1111/jeu.70087

Francine de V Rigo, Ana V D Porto, Eduardo Eizirik, Laura R P Utz

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The Morphology and Small Subunit rDNA Gene Phylogeny of the Novel Goniomonad Genus Ebisugoniomonas and Two Novel Poseidogoniomonas Species. 新型单胞菌属Ebisugoniomonas和两种新型波塞冬单胞菌的形态和rDNA基因系统发育

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-01 DOI: 10.1111/jeu.70088

Daryna Zavadska, Maria Sachs, Hartmut Arndt, Daniel J Richter

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Eukaryovorous Predation in Evolutionarily Significant Excavate-Like Flagellates. 进化上重要的挖形鞭毛虫的真核捕食。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-01 DOI: 10.1111/jeu.70084

Sei Suzuki-Tellier, James F Cass, Elizabeth J Weston, Kirsty Y Wan, Alastair G B Simpson, Thomas Kiørboe

{"title":"Eukaryovorous Predation in Evolutionarily Significant Excavate-Like Flagellates.","authors":"Sei Suzuki-Tellier, James F Cass, Elizabeth J Weston, Kirsty Y Wan, Alastair G B Simpson, Thomas Kiørboe","doi":"10.1111/jeu.70084","DOIUrl":"10.1111/jeu.70084","url":null,"abstract":"Accumulating evidence suggests that the Last Eukaryotic Common Ancestor (LECA) resembled contemporary excavates, a non-monophyletic group of deep branching flagellates. Here we explore the functional ecology of distantly related deep-branching \"excavate-like\" flagellates (in Alveolata and Provora) that share with many excavates a vane-bearing posterior flagellum beating in association with a ventral groove. This arrangement is key to generating a feeding current in the bacterivorous typical excavates. However, the four species examined here are motile \"hunters\" that randomly encounter eukaryotic prey. Initial prey attachment may be facilitated by the force generated by the vaned flagellum but eventually by firing extrusomes. Propulsion is driven by the two flagella that are directed backwards. Despite minor differences, the propulsion and hunting strategies are similar between these two deep-branching groups and distinctly different from those found in typical excavates. While the vane-and-groove morphology may help prey attachment, simulations show that it seems energetically disadvantageous to propulsion. Thus, its overall role, origin, and phylogenetic implications remain unclear. This study adds to our knowledge of the functional ecological range of living vane- and groove-bearing flagellates. Given their significance to understanding early eukaryote evolutionary history, this may in turn shed light on the deep history of eukaryote life.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 3","pages":"e70084"},"PeriodicalIF":3.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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mTOR-Dependent Autophagy Is Conserved in the Ciliate Tetrahymena thermophila. mtor依赖性自噬在纤毛虫嗜热四膜虫中是保守的。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-01 DOI: 10.1111/jeu.70086

Shinya Matsuda, Kentaro Nakano

{"title":"mTOR-Dependent Autophagy Is Conserved in the Ciliate Tetrahymena thermophila.","authors":"Shinya Matsuda, Kentaro Nakano","doi":"10.1111/jeu.70086","DOIUrl":"10.1111/jeu.70086","url":null,"abstract":"In Tetrahymena, autophagy appears to be involved in the integrity of the mitochondria which supply energy for active ciliary movement on the cell surface, the maintenance of various structures such as cilia, and cell shape changes associated with starvation. In this organism, which is evolutionarily distant from yeast and animal cells, the mechanisms controlling autophagy remain largely unknown. mTOR is a major regulatory factor that suppresses autophagy induction when cells are under nutrient-rich conditions. Previous studies suggest that T. thermophila contains two mTOR orthologs, but lacks the canonical TORC1 subunits, while retaining TORC2-associated components. To determine whether mTOR controls autophagy in T. thermophila, we examined Torin1, an ATP-competitive inhibitor that blocks mTOR kinase activity regardless of complex composition. It markedly triggered ATG8 puncta formation and mitochondrial degradation under nutrient-rich conditions. In contrast, rapamycin, a TORC1-specific inhibitor widely used to induce autophagy, did not affect cell growth or autophagy. These findings demonstrate that mTOR functions as a negative regulator of autophagy in T. thermophila, likely through a rapamycin-insensitive TORC2 or noncanonical complex. Our results highlight lineage-specific divergence in mTOR signaling architecture and expand our understanding of the diversity of autophagy regulation across eukaryotes.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 3","pages":"e70086"},"PeriodicalIF":3.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147930361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Comparative Analysis of Gut Eukaryotic Communities in Three Laboratory-Reared Cockroach Species Using Metabarcoding. 三种实验室饲养蜚蠊肠道真核生物群落的元条形码比较分析。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-05-01 DOI: 10.1111/jeu.70081

Dongjun Kang, Xavier Chavarria, Jun Ho Choi, Myung-Hee Yi, Yun Soo Jang, Singeun Oh, Du-Yeol Choi, Arwa Shatta, Sohyeon Yun, Myungjun Kim, Seongjun Choe, Tai-Soon Yong, Ju Yeong Kim

{"title":"Comparative Analysis of Gut Eukaryotic Communities in Three Laboratory-Reared Cockroach Species Using Metabarcoding.","authors":"Dongjun Kang, Xavier Chavarria, Jun Ho Choi, Myung-Hee Yi, Yun Soo Jang, Singeun Oh, Du-Yeol Choi, Arwa Shatta, Sohyeon Yun, Myungjun Kim, Seongjun Choe, Tai-Soon Yong, Ju Yeong Kim","doi":"10.1111/jeu.70081","DOIUrl":"10.1111/jeu.70081","url":null,"abstract":"Cockroaches are known reservoirs for diverse bacterial microbiomes. However, comprehensive analyses of the eukaryotic communities within cockroaches remain limited. In this study, we selected three long-term laboratory-reared cockroach species (Blattella germanica, Periplaneta fuliginosa, and Periplaneta japonica) and performed metabarcoding of the 18S rRNA V9 region using the iSeq 100 platform. The nematode Blatticola blattae was identified in B. germanica, and Leidynema appendiculata was found in both P. fuliginosa and P. japonica. The amplicon sequence variant (ASV) of Nyctotherus (Ciliophora) was detected in all three species of cockroach, while Entamoeba sp. was detected in P. fuliginosa and P. japonica. Compared with the other two species, B. germanica exhibited a higher prevalence of the fungus Nephridiophaga. Our findings showed that laboratory-reared cockroaches belonging to different species harbored distinct commensal and parasitic eukaryotic taxa. While most ASVs were of reduced clinical concern for humans as opposed to studies using wild-caught specimens, the detection of commensals, arthropod parasites and potential human pathogens illustrates the complexity of the cockroach-associated eukaryome and the influence of host identity. Our study emphasizes the importance of host-specificity in the eukaryotic community of laboratory-reared cockroach models.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 3","pages":"e70081"},"PeriodicalIF":3.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0