Journal of Eukaryotic Microbiology最新文献_第5页

Novel cytoskeletal traits in the intestinal parasites (Squirmida, Platyproteum vivax) of Pacific peanut worms (Sipuncula, Phascolosoma agassizii) 太平洋花生蠕虫（Sipuncula, Phascolosoma agassizii）肠道寄生虫（Squirmida, Platyproteum vivax）的新细胞骨架特征。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-02-25 DOI: 10.1111/jeu.13023

Danja Currie-Olsen, Brian S. Leander

{"title":"Novel cytoskeletal traits in the intestinal parasites (Squirmida, Platyproteum vivax) of Pacific peanut worms (Sipuncula, Phascolosoma agassizii)","authors":"Danja Currie-Olsen, Brian S. Leander","doi":"10.1111/jeu.13023","DOIUrl":"10.1111/jeu.13023","url":null,"abstract":"The cytoskeletal organization of a squirmid, namely Platyproteum vivax, was investigated with confocal laser scanning microscopy (CLSM) to refine inferences about convergent evolution among intestinal parasites of marine invertebrates. Platyproteum inhabits Pacific peanut worms (Phascolosoma agassizii) and has traits that are similar to other lineages of myzozoan parasites, namely gregarine apicomplexans within Selenidium, such as conspicuous feeding stages, called “trophozoites,” capable of dynamic undulations. SEM and CLSM of P. vivax revealed an inconspicuous flagellar apparatus and a uniform array of longitudinal microtubules organized in bundles (LMBs). Extreme flattening of the trophozoites and a consistently oblique morphology of the anterior end provided a reliable way to distinguish dorsal and ventral surfaces. CLSM revealed a novel system of microtubules oriented in the flattened dorsoventral plane. Most of these dorsoventral microtubule bundles (DVMBs) had a punctate distribution and were evenly spaced along a curved line spanning the longitudinal axis of the trophozoites. This configuration of microtubules is inferred to function in maintaining the flattened shape of the trophozoites and facilitate dynamic undulations. The novel traits in Platyproteum are consistent with phylogenomic data showing that this lineage is only distantly related to Selenidium and other marine gregarine apicomplexans with dynamic intestinal trophozoites.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139944206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous reproduction of planktonic foraminifera in laboratory culture 浮游有孔虫在实验室培养中的连续繁殖。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-02-25 DOI: 10.1111/jeu.13022

Julie Meilland, Michael Siccha, Raphaël Morard, Michal Kucera

{"title":"Continuous reproduction of planktonic foraminifera in laboratory culture","authors":"Julie Meilland, Michael Siccha, Raphaël Morard, Michal Kucera","doi":"10.1111/jeu.13022","DOIUrl":"10.1111/jeu.13022","url":null,"abstract":"Planktonic foraminifera were long considered obligate sexual outbreeders but recent observations have shown that nonspinose species can reproduce by multiple fission. The frequency of multiple fission appears low but the survival rate of the offspring is high and specimens approaching fission can be distinguished. We made use of this observation and established a culturing protocol aimed at enhancing the detection and frequency of fission. Using this protocol, we selectively cultured specimens of Neogloboquadrina pachyderma and raised the frequency of reproduction by fission in culture from 3% in randomly selected specimens to almost 60%. By feeding the resulting offspring different strains of live diatoms, we obtained a thriving offspring population and during the subsequent 6 months of culturing, we observed two more successive generations produced by fission. This provides evidence that in nonspinose species of planktonic foraminifera, reproduction by multiple fission is likely clonal and corresponds to the schizont phase known from benthic foraminifera. We subsequently tested if a similar culturing strategy could be applied to Globigerinita glutinata, representing a different clade of planktonic foraminifera, and we were indeed able to obtain offspring via multiple fission in this species. This work opens new avenues for laboratory-based experimental work with planktonic foraminifera.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139944205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Living in the cracks: Two novel genera of Variosea (Amoebozoa) discovered on an urban sidewalk 生活在裂缝中：在城市人行道上发现的两个新的变形虫属。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-01-19 DOI: 10.1111/jeu.13020

Nicholas Fry, Gabriel A. Schuler, Robert E. Jones, Peter G. Kooienga, Violet Jira, Maggie Shepherd, Alexander K. Tice, Matthew W. Brown

{"title":"Living in the cracks: Two novel genera of Variosea (Amoebozoa) discovered on an urban sidewalk","authors":"Nicholas Fry, Gabriel A. Schuler, Robert E. Jones, Peter G. Kooienga, Violet Jira, Maggie Shepherd, Alexander K. Tice, Matthew W. Brown","doi":"10.1111/jeu.13020","DOIUrl":"10.1111/jeu.13020","url":null,"abstract":"Biological soil crusts represent a rich habitat for diverse and complex eukaryotic microbial communities. A unique but extremely common habitat is the urban sidewalk and its cracks that collect detritus. While these habitats are ubiquitous across the globe, little to no work has been conducted to characterize protists found there. Amoeboid protists are major predators of bacteria and other microbial eukaryotes in these microhabitats and therefore play a substantial ecological role. From sidewalk crack soil crusts, we have isolated three naked amoebae with finely tapered subpseudopodia, and a simple life cycle consisting of a trophic amoeba and a cyst stage. Using a holistic approach including light, electron, and fluorescence microscopy as well as phylogenetics using the ribosomal small subunit rRNA gene and phylogenomics using 230 nuclear genes, we find that these amoeboid organisms fail to match any previously described eukaryote genus. However, we determined the amoebae belong to the amoebozoan lineage Variosea based on phylogenetics. The molecular analyses place our isolates in two novel genera forming a grade at the base of the variosean group Protosteliida. These three novel varioseans among two novel genera and species are herein named “Kanabo kenzan” and “Parakanabo toge.”","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First molecular characterization of Blastocystis subtypes from domestic animals (sheep and cattle) and their animal-keepers in Ilam, western Iran: A zoonotic concern 伊朗西部伊拉姆地区家畜（绵羊和牛）及其饲养者身上首次出现的布氏囊虫亚型的分子特征：人畜共患病问题。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-01-15 DOI: 10.1111/jeu.13019

Morteza Shams, Ali Mohammad Bahrami, Asma Mousivand, Laya Shamsi, Ali Asghari, Saeed Shahabi, Alireza Sadrebazzaz

{"title":"First molecular characterization of Blastocystis subtypes from domestic animals (sheep and cattle) and their animal-keepers in Ilam, western Iran: A zoonotic concern","authors":"Morteza Shams, Ali Mohammad Bahrami, Asma Mousivand, Laya Shamsi, Ali Asghari, Saeed Shahabi, Alireza Sadrebazzaz","doi":"10.1111/jeu.13019","DOIUrl":"10.1111/jeu.13019","url":null,"abstract":"A total of 360 fecal samples were randomly collected from 150 cattle, 150 sheep, and 60 humans (30 people with close animal contact and 30 individuals without close animal contact) at 10 farms in Ilam, western Iran from June 2022 to August 2023. All samples were directly examined for Blastocystis by zinc sulfate flotation, followed by microscopic observation. Positive samples were further subtyped using conventional PCR and sequencing methods. A mean prevalence of 5.3% (16/300) was estimated for Blastocystis infection among examined animals, with 6% and 4.7% for cattle and sheep, respectively. Among the people who had close and non-close animal contact, 16.7% (5/30) and 3.3% (1/30) were infected with Blastocystis, respectively (p < 0.05). All 22 positive samples were successfully sequenced at the SSU rRNA locus. Accordingly, Blastocystis isolates infecting domestic animals in Ilam belonged to the four STs (ST1-ST3, and ST10). Of the 16 animal isolates, nine sequences (four ST10, three ST3, and two ST1) were related to cattle, and seven sequences (three ST10, two ST3, and two ST2) were isolated from sheep. Among the six human isolates, ST3 was the most predominant ST, followed by STs 1, 2, 6, and 7 (one case each). Of note, ST1-ST3 were isolated in various farms both from animals and their breeders, which indicates the possible circulation of these STs between animal and human populations.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139471940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grazing preferences of three species of amoebae on cyanobacteria and green algae 三种变形虫对蓝藻和绿藻的偏好。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-01-10 DOI: 10.1111/jeu.13018

Harold G. Weger, April K. Polasek, Derek M. Wright, Arun Damodaran, John Stavrinides

{"title":"Grazing preferences of three species of amoebae on cyanobacteria and green algae","authors":"Harold G. Weger, April K. Polasek, Derek M. Wright, Arun Damodaran, John Stavrinides","doi":"10.1111/jeu.13018","DOIUrl":"10.1111/jeu.13018","url":null,"abstract":"Twenty species/isolates of cyanobacteria and green algae were isolated from cyanobacterial bloom samples in lakes associated with the upper Qu'Appelle River drainage system in southern Saskatchewan, Canada. Three amoebae species (Cochliopodium sp., Vannella sp. and Vermamoeba vermiformis) were also isolated from one of these samples, and were subjected to grazing assays to determine which species of cyanobacteria or algae could potentially serve as a food source. Amoeba grazing rates were quantified based on the diameter of the plaque after 12 days on agar plate assays, and by estimation of the amoeba population growth rate from the rate of increase of plaque area. The common cyanobacterial bloom-formers Dolichospermum sp. and Aphanizomenon flos-aquae supported high growth rates for all three amoebae, while green algae, with the exception of one green alga/amoeba combination, did not support growth of the tested amoebae. Many of the cyanobacterial and algal isolates that did not support amoebae growth were ingested, suggesting that ingestion did not determine grazing success. Overall, while the cyanobacteria Dolichospermum sp. and Aphanizomenon flos-aquae were suitable food sources for the amoebae, the other cyanobacteria were grazed in an unpredictable manner, with some species/strains grazed by some amoebae and some species not grazed at all.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139403044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The function of the feeding groove of ‘typical excavate’ flagellates 典型挖掘型 "鞭毛虫摄食槽的功能

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-18 DOI: 10.1111/jeu.13016

Sei Suzuki-Tellier, Thomas Kiørboe, Alastair G. B. Simpson

{"title":"The function of the feeding groove of ‘typical excavate’ flagellates","authors":"Sei Suzuki-Tellier, Thomas Kiørboe, Alastair G. B. Simpson","doi":"10.1111/jeu.13016","DOIUrl":"10.1111/jeu.13016","url":null,"abstract":"Phagotrophic flagellates are the main consumers of bacteria and picophytoplankton. Despite their ecological significance in the ‘microbial loop’, many of their predation mechanisms remain unclear. ‘Typical excavates’ bear a ventral groove, where prey is captured for ingestion. The consequences of feeding through a ‘semi-rigid’ furrow on the prey size range have not been explored. An unidentified moving element called ‘the wave’ that sweeps along the bottom of the groove toward the site of phagocytosis has been observed in a few species; its function is unclear. We investigated the presence, behavior, and function of the wave in four species from the three excavate clades (Discoba, Metamonada, and Malawimonadida) and found it present in all studied cases, suggesting the potential homology of this feature across all three groups. The wave displayed a species-specific behavior and was crucial for phagocytosis. The morphology of the feeding groove had an upper-prey size limit for successful prey captures, but smaller particles were not constrained. Additionally, the ingestion efficiencies were species dependent. By jointly studying these feeding traits, we speculate on adaptations to differences in food availability to better understand their ecological functions.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138716896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The second most abundant dinophyte in the ponds of a botanical garden is a species new to science 植物园池塘中数量第二多的双子叶植物是科学界的新物种

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-11 DOI: 10.1111/jeu.13015

Anna Müller, Marina Stark, Sophia Schottenhammel, Uwe John, Juliana Chacón, Andreas Klingl, Victoria Julia Christine Holzer, Marika Schöffer, Marc Gottschling

{"title":"The second most abundant dinophyte in the ponds of a botanical garden is a species new to science","authors":"Anna Müller, Marina Stark, Sophia Schottenhammel, Uwe John, Juliana Chacón, Andreas Klingl, Victoria Julia Christine Holzer, Marika Schöffer, Marc Gottschling","doi":"10.1111/jeu.13015","DOIUrl":"10.1111/jeu.13015","url":null,"abstract":"In the microscopy realm, a large body of dark biodiversity still awaits to be uncovered. Unarmoured dinophytes are particularly neglected here, as they only present inconspicuous traits. In a remote German locality, we collected cells, from which a monoclonal strain was established, to study morphology using light and electron microscopy and to gain DNA sequences from the rRNA operon. In parallel, we detected unicellular eukaryotes in ponds of the Botanical Garden Munich-Nymphenburg by DNA-metabarcoding (V4 region of the 18S rRNA gene), weekly sampled over the course of a year. Strain GeoK*077 turned out to be a new species of Borghiella with a distinct position in molecular phylogenetics and characteristic coccoid cells of ovoid shape as the most important diagnostic trait. Borghiella ovum, sp. nov., was also present in artificial ponds of the Botanical Garden and was the second most abundant dinophyte detected in the samples. More specifically, Borghiella ovum, sp. nov., shows a clear seasonality, with high frequency during winter months and complete absence during summer months. The study underlines the necessity to assess the biodiversity, particularly of the microscopy realm more ambitiously, if even common species such as formerly Borghiella ovum are yet unknown to science.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs 镰刀形艾美耳菌细胞外囊泡表达宿主细胞lncRNA的差异

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-10 DOI: 10.1111/jeu.13009

Joshua S. Olajide, Zigang Qu, Shunli Yang, Bin Yang, Xiao Xu, Jing Wang, Jianping Cai

{"title":"Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs","authors":"Joshua S. Olajide, Zigang Qu, Shunli Yang, Bin Yang, Xiao Xu, Jing Wang, Jianping Cai","doi":"10.1111/jeu.13009","DOIUrl":"10.1111/jeu.13009","url":null,"abstract":"Long noncoding RNAs (lncRNAs) are regulatory transcripts during protozoan infections in the host intestinal epithelial cells (IECs). Apicomplexan Eimeria falciformis sporozoite extracellular vesicles (EVs) contain virulence factors that modulate host IECs pro-inflammatory genes and immune responses. In this study, E. falciformis sporozoites were made to interact with inactivated host cells, and the parasite EVs were separated from total secretome by ultracentrifugation and purified on density gradient medium. Dose-dependent bio-activity of E. falciformis EVs was investigated by RNA sequencing, functional annotation and quantitative PCR. It was found that E. falciformis EVs induced mRNA, circRNA, and lncRNA expressions in mouse IECs. Of 38, 217 lncRNAs assembled, 157 and 152 were upwardly and downwardly expressed respectively. Differentially expressed lncRNAs were associated with cytokines, pyroptosis, and immune signaling pathways including FoxO, NF-κB, MAPK, and TGF-β. In essence, E. falciformis EVs altered host cell RNA expressions during the interaction with host IECs. Also, differentially expressed lncRNAs are potential diagnostic transcripts during Eimeria infections.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to the article “On the phylogenetic position of Raphidocystis pallida with some notes on its life cycle” 更正。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-08 DOI: 10.1111/jeu.13011

引用次数: 0

In memoriam: Thomas Cavalier-Smith (1942–2021) 纪念:托马斯·卡瓦利-史密斯(1942-2021)。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-07 DOI: 10.1111/jeu.13013

Gaytha A. Langlois, Sonja Rueckert

引用次数: 0