Journal of Eukaryotic Microbiology最新文献

{"title":"Morphology and phylogenetic position of three anaerobic ciliates from the classes Odontostomatea and Muranotrichea (Ciliophora)","authors":"Daniel Méndez-Sánchez,&nbsp;Ondřej Pomahač,&nbsp;Johana Rotterová,&nbsp;William A. Bourland,&nbsp;Ivan Čepička","doi":"10.1111/jeu.12965","DOIUrl":"10.1111/jeu.12965","url":null,"abstract":"<p>The diversity of the classes Odontostomatea and Muranotrichea, which contain solely obligate anaerobes, is poorly understood. We studied two populations of <i>Mylestoma</i> sp., one of <i>Saprodinium dentatum</i> (Odontostomatea), two of <i>Muranothrix felix</i> sp. nov., and one of <i>Muranothrix</i> sp. (Muranotrichea) employing live observation, protargol impregnation, scanning electron microscopy, and 18S rRNA gene sequencing. Conspecificity of <i>Mylestoma</i> sp., described here, with a previously described species of this genus cannot be excluded since no species have been studied with modern methods. Phylogenetically, the genus <i>Mylestoma</i> is closely related to the odontostomatid <i>Discomorphella pedroeneasi</i>, although the phylogenetic position of class Odontostomatea itself remains unresolved. The newly described muranotrichean species, <i>Muranothrix felix</i> sp. nov., is morphologically similar to <i>M</i>. <i>gubernata</i> but can be distinguished by its fewer macronuclear nodules and fewer adoral membranelles; moreover, it is clearly distinguished from <i>M. gubernata</i> by its 18S rRNA gene sequence. Another population, designated here as <i>Muranothrix</i> sp., most likely represents a separate species.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.12965","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9996133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the interactive effects of temperature, pH, and salinity on Naegleria fowleri persistence","authors":"Leigha M. Stahl,&nbsp;Julie B. Olson","doi":"10.1111/jeu.12964","DOIUrl":"10.1111/jeu.12964","url":null,"abstract":"<p><i>Naegleria fowleri</i> causes primary amoebic meningoencephalitis, a deadly infection that occurs when free-living amoebae enter the nose via freshwater and travel to the brain. <i>N</i>. <i>fowleri</i> naturally thrives in freshwater and soil and is thought to be associated with elevated water temperatures. While environmental and laboratory studies have sought to identify what environmental factors influence its presence, many questions remain. This study investigated the interactive effects of temperature, pH, and salinity on <i>N</i>. <i>fowleri</i> in deionized and environmental waters. Three temperatures (15, 25, 35°C), pH values (6.5, 7.5, 8.5), and salinity concentrations (0.5%, 1.5%, 2.5% NaCl) were used to evaluate the growth of <i>N</i>. <i>fowleri</i> via ATP luminescent assays. Results indicated <i>N</i>. <i>fowleri</i> grew best at 25°C, and multiple interactive effects occurred between abiotic factors. Interactions varied slightly by water type but were largely driven by temperature and salinity. Lower temperature increased <i>N</i>. <i>fowleri</i> persistence at higher salinity levels, while low salinity (0.5% NaCl) supported <i>N</i>. <i>fowleri</i> growth at all temperatures. This research provided an experimental approach to assess interactive effects influencing the persistence of <i>N</i>. <i>fowleri</i>. As climate change impacts water temperatures and conditions, understanding the microbial ecology of <i>N</i>. <i>fowleri</i> will be needed minimize pathogen exposure.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9626763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene","authors":"Fakun Li,&nbsp;Yangyang Deng,&nbsp;Wanxin Sheng,&nbsp;Xihui Gao,&nbsp;Weijuan Wang,&nbsp;Zhili Chu,&nbsp;Xuefang Mei,&nbsp;Zhenke Yang,&nbsp;Xiaowei Tian,&nbsp;Shuai Wang,&nbsp;Zhenchao Zhang","doi":"10.1111/jeu.12963","DOIUrl":"10.1111/jeu.12963","url":null,"abstract":"<p>Trichomoniasis is a common and curable sexually transmitted disease worldwide. The rapid, convenient, and accurate diagnosis of trichomoniasis is an important link in the prevention and treatment of the disease. The current detection methods of <i>Trichomonas vaginalis</i> are mainly wet mount microscopy, culture, nested PCR, and loop-mediated isothermal amplification. However, these detection methods have some shortcomings. In this study, a recombinant enzyme polymerase amplification (RPA) assay had been conducted to detect <i>T. vaginalis</i>. The target gene and the corresponding primers were screened, and the reaction system and conditions were optimized in the assay of RPA. The sensitivity and specificity of this detection method were analyzed. The detection efficiency of wet mount microscopy, culture, nested PCR, and RPA was compared by testing 53 clinical samples from vaginal secretions. By screening, the actin gene of <i>T. vaginalis</i> could be used as a target gene for RPA detection of <i>T. vaginalis</i>, and the optimum reaction condition to amplify the actin gene by RPA was at 39°C for 30 min. The detection limit of <i>T. vaginalis DNA</i> using RPA was 1 pg, corresponding to a sensitivity of approximately five trophozoites. The RPA assay demonstrated high specificity for <i>T. vaginalis</i>, and there was no cross-reactivity with <i>Giardia lamblia</i>, <i>Escherichia coli</i>, <i>Lactobacillus</i>, <i>Toxoplasma gondii</i>, <i>Staphylococcus aureus</i>, and <i>Candida albicans</i>. Of the 53 clinical samples, the positive rates of <i>T. vaginalis</i> detected by wet mount microscopy, culture, nested PCR and RPA were 50.9 4% (27/53), 71.7% (38/53), 71.7% (38/53), and 69.81% (37/53), respectively. Compared with culture which was used as the gold standard for diagnosing trichomoniasis, testing clinical samples by wet mount microscopy showed 71.05% sensitivity, 100% specificity, and moderate diagnostic agreement with the culture (<i>K</i> = 0.581, <i>Z</i> = 4.661, <i>p</i> &lt; 0.001). The nested PCR showed 100% sensitivity, 100% specificity, and excellent diagnostic agreement (<i>K</i> = 1, <i>Z</i> = 7.28, <i>p</i> &lt; 0.001), while RPA displayed 97.37% sensitivity, 100% specificity, and excellent diagnostic agreement (<i>K</i> = 0.954, Z = 6.956, <i>p</i> &lt; 0.001). At the present study, rapid amplification of actin gene by RPA could be used as a tool for detection of <i>T. vaginalis</i>. The detection method of RPA was more sensitive than wet mount microscopy and displayed excellent specificity. Moreover, RPA amplification of actin gene did not require a PCR instrument and the amplification time was shorter than that of ordinary PCR. Therefore, the RPA assay was proposed in this study as a point-of-care examination and a diagnostic method of <i>T. vaginalis</i> infection, which exhibited the potential value in the treatment and prevention of trichomoniasis.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9626750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phaeogromids of the mesopelagic marine plankton: Temporal variability of concentrations and observations of feeding structures of four species from the mesopelagic in the Mediterranean Sea","authors":"John R. Dolan,&nbsp;Laurent Coppola","doi":"10.1111/jeu.12962","DOIUrl":"10.1111/jeu.12962","url":null,"abstract":"<p>Challengerids, phaeogromids rhizarian protists, are emblematic protists of the deep sea but are also enigmatic as they occur in very low concentrations. In previous studies, we reported on temporal changes in abundance at a near-shore mesopelagic site, but only as part of sampling of the entire microplankton assemblage, not well-suited for examining phaeogromids. Consequently, we turned to using a closing plankton net to provide material from large volumes of seawater, thus allowing for more robust estimates of concentrations and material for observations of living cells, to our knowledge the first made. Here, we report our results on the four most commonly occurring species: <i>Challengeranium diadon</i>, <i>Challengereron willemoesii</i>, <i>Challengeria xiphodon</i>, and <i>Euphysetta lucani</i>. In contrast to our previous report, we found that changes in concentrations were not related to water column stratification, and the four species roughly co-varied with time. Observations of live cells revealed that all four species deploy tentacle-like pseudopods and also very large unstructured webs of fine pseudopods. The similarities in feeding webs suggest similar prey are exploited, and the similar temporal changes in abundances suggest a common factor or factors (unknown at this time) govern their concentrations. Films of live cells are provided in Supplementary Files.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9614962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of multicellular colonies by choanoflagellates increases susceptibility to capture by amoeboid predators","authors":"Nicole E. Chin,&nbsp;Tiffany C. Wu,&nbsp;J. Michael O'Toole,&nbsp;Kevin Xu,&nbsp;Tom Hata,&nbsp;Mimi A. R. Koehl","doi":"10.1111/jeu.12961","DOIUrl":"10.1111/jeu.12961","url":null,"abstract":"<p>Many heterotrophic microbial eukaryotes are size-selective feeders. Some microorganisms increase their size by forming multicellular colonies. We used choanoflagellates, <i>Salpingoeca helianthica</i>, which can be unicellular or form multicellular colonies, to study the effects of multicellularity on vulnerability to predation by the raptorial protozoan predator, <i>Amoeba proteus</i>, which captures prey with pseudopodia. Videomicrography used to measure the behavior of interacting <i>S. helianthica</i> and <i>A. proteus</i> revealed that large choanoflagellate colonies were more susceptible to capture than were small colonies or single cells. Swimming colonies produced larger flow fields than did swimming unicellular choanoflagellates, and the distance of <i>S. helianthica</i> from <i>A. proteus</i> when pseudopod formation started was greater for colonies than for single cells. Prey size did not affect the number of pseudopodia formed and the time between their formation, pulsatile kinematics and speed of extension by pseudopodia, or percent of prey lost by the predator. <i>S. helianthica</i> did not change swimming speed or execute escape maneuvers in response to being pursued by pseudopodia, so size-selective feeding by <i>A. proteus</i> was due to predator behavior rather than prey escape. Our results do not support the theory that the selective advantage of becoming multicellular by choanoflagellate-like ancestors of animals was reduced susceptibility to protozoan predation.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9623810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular prevalence and subtypes distribution of Blastocystis sp. among outpatients and inpatients in north and south areas of Henan Province, China","authors":"Xuefang Mei,&nbsp;Changwei Su,&nbsp;Weijuan Wang,&nbsp;Bo Zhang,&nbsp;Lai Wei,&nbsp;Zhenchao Zhang,&nbsp;Xiaowei Tian,&nbsp;Zhenke Yang,&nbsp;Xiangrui Li,&nbsp;Aijun Duan,&nbsp;Shuai Wang","doi":"10.1111/jeu.12960","DOIUrl":"10.1111/jeu.12960","url":null,"abstract":"<p><i>Blastocystis</i> sp<i>.</i> is one of the most common intestinal parasites in humans and many animals. To further understand the infection of <i>Blastocystis hominis</i> (<i>B. hominis</i>) and the distribution of its genotype in some areas of Henan Province, China, 793 stool samples from outpatients and inpatients in Xinxiang City and Xinyang City, Henan Province were collected from April 2020 to July 2022. The samples were detected by polymerase chain reaction and analyzed by univariate analysis and logistic regression analysis. The results showed that the infection rates of <i>B. hominis</i> in Xinxiang and Xinyang were 10.97% (51/465) and 10.98% (36/328), respectively. Although there were no significant differences in <i>B. hominis</i> infection between gender, age, residence, and disease background, the incidence of hematochezia significantly differed from the incidence of abdominal pain, diarrhea, and constipation among participants (χ² = 15.795, <i>p</i> = 0.002). A total of 87 positive samples were sequenced and compared with Basic Local Alignment Search Tool, and five subtypes (ST1, ST3, ST4, ST6, and ST7) were identified, of which ST3 was the dominant subtype (63.22%, 55/87), followed by ST7 (17.24%, 15/87) and ST1 (16.09%, 14/87). This is the first study that analyzed the prevalence and subtype distribution of <i>B. hominis</i> in southern and northern Henan Province, thus providing new insights into the epidemiology of <i>B. hominis</i>.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9621120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"18S rDNA sequence–structure phylogeny of the Euglenophyceae (Euglenozoa, Euglenida)","authors":"Antonia S. Rackevei,&nbsp;Anna Karnkowska,&nbsp;Matthias Wolf","doi":"10.1111/jeu.12959","DOIUrl":"10.1111/jeu.12959","url":null,"abstract":"<p>The phylogeny of <i>Euglenophyceae</i> (<i>Euglenozoa</i>, <i>Euglenida</i>) has been discussed for decades with new genera being described in the last few years. In this study, we reconstruct a phylogeny using 18S rDNA sequence and structural data simultaneously. Using homology modeling, individual secondary structures were predicted. Sequence–structure data are encoded and automatically aligned. Here, we present a sequence–structure neighbor-joining tree of more than 300 taxa classified as <i>Euglenophyceae</i>. Profile neighbor-joining was used to resolve the basal branching pattern. Neighbor-joining, maximum parsimony, and maximum likelihood analyses were performed using sequence–structure information for manually chosen subsets. All analyses supported the monophyly of <i>Eutreptiella</i>, <i>Discoplastis</i>, <i>Lepocinclis</i>, <i>Strombomonas</i>, <i>Cryptoglena</i>, <i>Monomorphina</i>, <i>Euglenaria</i>, and <i>Colacium</i>. Well-supported topologies were generally consistent with previous studies using a combined dataset of genetic markers. Our study supports the simultaneous use of sequence and structural data to reconstruct more accurate and robust trees. The average bootstrap value is significantly higher than the average bootstrap value obtained from sequence-only analyses, which is promising for resolving relationships between more closely related taxa.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.12959","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9605956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel insights into molecular mechanisms of vegetative cell cycle and resting cyst formation in Apodileptus cf. visscheri (Alveolata, Ciliophora)","authors":"Jiahui Xu,&nbsp;Zhuo Shen,&nbsp;Minjie Yu,&nbsp;Yalan Sheng,&nbsp;Zhenzhen Yi","doi":"10.1111/jeu.12958","DOIUrl":"10.1111/jeu.12958","url":null,"abstract":"<p>Ciliates usually with big cell sizes, complex morphological structures, and diverse life cycles, are good model organisms for studying cell proliferation regulation of eukaryotes. Up to date, the molecular regulation mechanisms for the vegetative cell cycle and encystment of these ciliates are poorly understood. Here, transcriptomes of <i>Apodileptus</i> cf. <i>visscheri</i>, which has an asexual vegetative cell cycle and is apt to encyst when environmental conditions become unfavorable, were sequenced to enrich our related knowledge. In this study, three replicates were sequenced for each of four cell stages, including initial period of growth, morphogenesis, cell division, and resting cyst. The significant transcription differences, involving cell cycle, biosynthesis, and energy metabolism pathways, were revealed between the resting cyst and vegetative cell cycle. Further investigations showed that the cell cycle pathway was enriched during morphogenesis stage and cell division stage. Compared to the initial period of growth stage, the differentially expressed genes involved in cellular components and molecular function were significantly enriched during cell division stage, while cellular components and biological processes were significantly enriched during morphogenesis stage. These provide novel insights into a comprehensive understanding at the molecular level of the survival and adaptive mechanism of unicellular eukaryotes.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9605942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the molecular and morphological diversity of Apusomonadida, a deep-branching group of gliding bacterivorous protists","authors":"Guifré Torruella,&nbsp;Luis Javier Galindo,&nbsp;David Moreira,&nbsp;Maria Ciobanu,&nbsp;Aaron A. Heiss,&nbsp;Naoji Yubuki,&nbsp;Eunsoo Kim,&nbsp;Purificación López-García","doi":"10.1111/jeu.12956","DOIUrl":"10.1111/jeu.12956","url":null,"abstract":"<p>Apusomonads are cosmopolitan bacterivorous biflagellate protists usually gliding on freshwater and marine sediment or wet soils. These nanoflagellates form a sister lineage to opisthokonts and may have retained ancestral features helpful to understanding the early evolution of this large supergroup. Although molecular environmental analyses indicate that apusomonads are genetically diverse, few species have been described. Here, we morphologically characterize 11 new apusomonad strains. Based on molecular phylogenetic analyses of the rRNA gene operon, we describe four new strains of the known species <i>Multimonas media</i>, <i>Podomonas capensis</i>, <i>Apusomonas proboscidea</i>, and <i>Apusomonas australiensis</i>, and rename <i>Thecamonas oxoniensis</i> as <i>Mylnikovia oxoniensis</i> n. gen., n. comb. Additionally, we describe four new genera and six new species: <i>Catacumbia lutetiensis</i> n. gen. n. sp., <i>Cavaliersmithia chaoae</i> n. gen. n. sp., <i>Singekia montserratensis</i> n. gen. n. sp., <i>Singekia franciliensis</i> n. gen. n. sp., <i>Karpovia croatica</i> n. gen. n. sp., and <i>Chelonemonas dolani</i> n. sp. Our comparative analysis suggests that apusomonad ancestor was a fusiform biflagellate with a dorsal pellicle, a plastic ventral surface, and a sleeve covering the anterior flagellum, that thrived in marine, possibly oxygen-poor, environments. It likely had a complex cell cycle with dormant and multiple fission stages, and sex. Our results extend known apusomonad diversity, allow updating their taxonomy, and provide elements to understand early eukaryotic evolution.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9242793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pontisma blauvikense sp. nov. the first member of the early-diverging oomycete genus Pontisma parasitizing brown algae","authors":"Anthony Buaya,&nbsp;Ichen Tsai,&nbsp;Marco Thines","doi":"10.1111/jeu.12957","DOIUrl":"10.1111/jeu.12957","url":null,"abstract":"<p>Holocarpic oomycetes have been neglected over several decades, until interest in these organisms has recently resurged. One of the most widespread genera of holocarpic oomycetes is <i>Pontisma</i>, parasitic to red seaweeds throughout all oceans. Recently, the genus <i>Sirolpidium</i> (parasitic to green algae) was found to be congeneric with <i>Pontisma</i>. This hinted at a high pathogenic versatility and prompted the screening of other macroalgae on the coastline of Iceland. During this survey a parasite of the brown algae <i>Pylaiella littoralis</i> was found, which formed anisolpidium-like thalli, but produced biflagellate zoospores. Phylogenetic investigations revealed that the parasite was placed in the genus <i>Pontisma</i>. In reconstructions based on partial nrSSU sequences, it grouped with some sequences of parasitoids of the diatom genus <i>Licmophora</i>, but the more variable mitochondrial <i>cox</i>2 sequences were divergent. Based on phylogenetic evidence and the unique parasitism of brown algae, the parasitoid is described as <i>Pontisma blauvikense</i> in this study. <i>Pontisma blauvikense</i> is the fourth oomycete species parasitic to <i>Pylaiella</i>, which is also parasitised by <i>Euychasma dicksonii</i> and two <i>Anisolpidium</i> species. For a better understanding of the ecology and evolution of holocarpic oomycetes, further research is necessary to investigate the host spectrum of <i>Pontisma</i> in general and <i>Pontisma blauvikense</i> in particular.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.12957","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9291325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}