Journal of Eukaryotic Microbiology最新文献_第10页

Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs 镰刀形艾美耳菌细胞外囊泡表达宿主细胞lncRNA的差异

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-10 DOI: 10.1111/jeu.13009

Joshua S. Olajide, Zigang Qu, Shunli Yang, Bin Yang, Xiao Xu, Jing Wang, Jianping Cai

{"title":"Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs","authors":"Joshua S. Olajide, Zigang Qu, Shunli Yang, Bin Yang, Xiao Xu, Jing Wang, Jianping Cai","doi":"10.1111/jeu.13009","DOIUrl":"10.1111/jeu.13009","url":null,"abstract":"Long noncoding RNAs (lncRNAs) are regulatory transcripts during protozoan infections in the host intestinal epithelial cells (IECs). Apicomplexan Eimeria falciformis sporozoite extracellular vesicles (EVs) contain virulence factors that modulate host IECs pro-inflammatory genes and immune responses. In this study, E. falciformis sporozoites were made to interact with inactivated host cells, and the parasite EVs were separated from total secretome by ultracentrifugation and purified on density gradient medium. Dose-dependent bio-activity of E. falciformis EVs was investigated by RNA sequencing, functional annotation and quantitative PCR. It was found that E. falciformis EVs induced mRNA, circRNA, and lncRNA expressions in mouse IECs. Of 38, 217 lncRNAs assembled, 157 and 152 were upwardly and downwardly expressed respectively. Differentially expressed lncRNAs were associated with cytokines, pyroptosis, and immune signaling pathways including FoxO, NF-κB, MAPK, and TGF-β. In essence, E. falciformis EVs altered host cell RNA expressions during the interaction with host IECs. Also, differentially expressed lncRNAs are potential diagnostic transcripts during Eimeria infections.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to the article “On the phylogenetic position of Raphidocystis pallida with some notes on its life cycle” 更正。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-08 DOI: 10.1111/jeu.13011

引用次数: 0

In memoriam: Thomas Cavalier-Smith (1942–2021) 纪念:托马斯·卡瓦利-史密斯(1942-2021)。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-07 DOI: 10.1111/jeu.13013

Gaytha A. Langlois, Sonja Rueckert

引用次数: 0

Morphology, morphogenesis, and molecular characterization of Castula specialis sp. nov. (Ciliophora, Armophorea, Metopida) Castula speciis sp. 11 .的形态、形态发生和分子特征(纤毛目、甲壳目、mettopida)。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-29 DOI: 10.1111/jeu.13014

Xiaochen Feng, Daniel Méndez-Sánchez, Wenbao Zhuang, Ran Li, Ondřej Pomahač, Ivan Čepička, Johana Rotterová, Xiaozhong Hu

{"title":"Morphology, morphogenesis, and molecular characterization of Castula specialis sp. nov. (Ciliophora, Armophorea, Metopida)","authors":"Xiaochen Feng, Daniel Méndez-Sánchez, Wenbao Zhuang, Ran Li, Ondřej Pomahač, Ivan Čepička, Johana Rotterová, Xiaozhong Hu","doi":"10.1111/jeu.13014","DOIUrl":"10.1111/jeu.13014","url":null,"abstract":"The morphology, morphogenesis, and molecular phylogeny of a new metopid ciliate, Castula specialis sp. nov., comprising three strains from geographically distant (China, Mexico, Czech Republic) anoxic freshwater habitats, were studied based on microscopic observation of live and protargol-stained specimens as well as SSU rRNA gene sequence data. The new species is characterized as follows: size in vivo 105–220 × 25–70 μm, body oblong to elongated ellipsoidal and asymmetrical; preoral dome distinctly projecting beyond the body; 32–46 adoral membranelles; 31–52 somatic kineties; and 4–7 setae. This study brings the first morphogenetic investigation of a member of the genus Castula. The morphogenesis of the type population (China) of the new species proceeds as in Metopus spp. comprising drastic changes in body shape and a pleurotelokinetal stomatogenesis; however, the main difference is the origin of the opisthe's paroral membrane that derives from all perizonal rows and some adjacent dome kineties. Phylogenetically, the genus Castula is paraphyletic.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang 新疆南部和田黑鸡囊虫属的分子特征。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-17 DOI: 10.1111/jeu.13012

Xinwei Feng, Luyao Xin, Bowen Zhang, Zhichao Wang, Zhu Meng, Fuchang Yu, Meng Qi

{"title":"Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang","authors":"Xinwei Feng, Luyao Xin, Bowen Zhang, Zhichao Wang, Zhu Meng, Fuchang Yu, Meng Qi","doi":"10.1111/jeu.13012","DOIUrl":"10.1111/jeu.13012","url":null,"abstract":"To determine the infection status and assess the zoonotic potential of Blastocystis spp. in Hotan Black chickens in southern Xinjiang, China, fecal samples were collected from 617 chickens on 18 large-scale farms. The presence of Blastocystis spp. was determined using polymerase chain reaction based on the small subunit rRNA (SSU rRNA) locus. The results revealed an overall infection rate of 26.3% (162/617). Samples from Farm 1 in Luopu County showed the highest infection rate (76.3%, 29/38). The highest and lowest infection rates were detected in the <30-day (34.4%, 43/125) and > 90-day age groups (12.4%, 11/89), respectively. The infection rate decreased with increasing age. Statistical analysis showed significant differences in the infection rates of Blastocystis spp. among the different sampling sites (p < 0.05) and age groups (p < 0.05). Four Blastocystis spp. subtypes (ST6, ST7, ST10, and ST23) were identified. The infection rates of the zoonotic subtypes, ST6 and ST7, were 3.2% (20/617) and 22.2% (137/617), respectively. The presence of Blastocystis spp. and zoonotic subtypes provided evidence for the potential transmission of this pathogen between Hotan Black chickens and humans, especially in animal handlers in this area.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136397686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhabdamoeba marina is a heterotrophic relative of chlorarachnid algae 卤虫是氯虫藻类的异养亲戚。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-08 DOI: 10.1111/jeu.13010

Takashi Shiratori, Ken-ichiro Ishida

引用次数: 0

Editorial Acknowledgment 编辑致谢。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-06 DOI: 10.1111/jeu.13006

引用次数: 0

Anaeramoeba pumila sp. nov. and Anaeramoeba sp. OCE22C represent two novel types of symbiosis of Anaeramoebae and prokaryotes Anaeramoeba pumila sp.nov.和Anaeramooba sp.OC22C代表Anaeramoepae和原核生物的两种新型共生。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-06 DOI: 10.1111/jeu.13008

Magdaléna Pavlátová, Pavla Hanousková, Ivan Čepička

引用次数: 0

Highly divergent morphology but a close molecular phylogenetic relationship between two little-known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species 形态高度分化，但两个鲜为人知的纤毛虫属Actinoblina和Papillorhabdos之间存在密切的分子系统发育关系（原生动物：Ciliophora：Litstomatea），并描述了两个新种。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-10-27 DOI: 10.1111/jeu.13007

Yong Chi, Danxu Tang, Jingtao Lei, Fan Wei, Saleh A. Al-Farraj, William A. Bourland, Zigui Chen

{"title":"Highly divergent morphology but a close molecular phylogenetic relationship between two little-known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species","authors":"Yong Chi, Danxu Tang, Jingtao Lei, Fan Wei, Saleh A. Al-Farraj, William A. Bourland, Zigui Chen","doi":"10.1111/jeu.13007","DOIUrl":"10.1111/jeu.13007","url":null,"abstract":"Free-living litostomatean ciliates, prominent microeukaryote predators commonly encountered in freshwater and marine habitats, play vital roles in maintaining energy flow and nutrient cycles. Nevertheless, understanding their biodiversity and phylogenetic relationships remains challenging due to insufficient morphological information and molecular data. As a new contribution to this group, three haptorian ciliates, including two new species (Actinobolina bivacuolata sp. nov. and Papillorhabdos foissneri sp. nov.) and the insufficiently described type species, Actinobolina radians, were isolated from wetlands around Lake Weishan, China and investigated by a combination of living morphology, stained preparations, and 18S rRNA gene sequence data. An illustrated key of the valid species within the two genera is provided. In addition, we reveal the phylogenetic positions of these two genera for the first time. Although they differ in all key morphologic characters such as general appearance (ellipsoidal with numerous tentacles vs. cylindrical), extrusomes (stored in tentacles vs. anchored to pellicle), circumoral kinety (present vs. absent), composition of somatic kineties (kinetosome clusters vs. monokinetids), and number of dorsal brush rows (1 vs. 4), they both cluster in a fully supported clade in the phylogenetic tree, which indicates that the biodiversity and additional molecular markers of this group need further exploration.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54229275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell DNA from West Greenland marine sediments suggests presence of Protoperidinium tricingulatum in the Arctic 从西格陵兰岛海洋沉积物中提取的单细胞DNA表明，在北极地区存在三角棘球藻。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-10-25 DOI: 10.1111/jeu.13005

Tiia Luostarinen, Sofia Ribeiro, Heike H. Zimmermann, Anna B. Kvorning, Maija Heikkilä

{"title":"Single-cell DNA from West Greenland marine sediments suggests presence of Protoperidinium tricingulatum in the Arctic","authors":"Tiia Luostarinen, Sofia Ribeiro, Heike H. Zimmermann, Anna B. Kvorning, Maija Heikkilä","doi":"10.1111/jeu.13005","DOIUrl":"10.1111/jeu.13005","url":null,"abstract":"Spiny brown dinoflagellate cysts are commonly used as sea-ice indicators in the Arctic, but their biological affinities are not well known. We present the first indication of hitherto temperate Protoperidinium tricingulatum in the Arctic based on single-cell LSU rDNA sequencing from sediments of the Disko Bay-Vaigat Sound, West Greenland. The morphological similarity of the sequenced cyst morphotype to the sea-ice indicator Islandinium? cezare morphotype 1 is striking. The morphology of the isolated cysts, as well as those observed in the total cyst assemblage following standard palynological preparation, both resemble either I.? cezare morphotype 1 or P. tricingulatum, suggesting that the specimens may in fact be close morphological variants of the same species. In addition, nine LSU rDNA sequences were obtained from morphological variants assigned to Islandinium minutum s.l.: including both subspecies minutum and subspecies barbatum. The two subspecies could not be differentiated based on partial LSU rDNA sequencing. Overall, Arctic spiny brown dinoflagellate cyst species may be morphologically more diverse and taxonomically more complex than shown earlier and further genetic and morphological studies are needed. Importantly, the value of cysts as palaeoecological indicators depends on a sound understanding of their biological affinity and taxonomy.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50158085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0