Journal of Eukaryotic Microbiology最新文献_第9页

Protozoa: The Overlooked Frontiers of Natural Antimicrobial Compounds 原生动物：天然抗菌化合物被忽视的前沿。

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-02-10 DOI: 10.1111/jeu.70068

Şevval Maral Özcan Aykol, Larasu Tuana Davşan

{"title":"Protozoa: The Overlooked Frontiers of Natural Antimicrobial Compounds","authors":"Şevval Maral Özcan Aykol, Larasu Tuana Davşan","doi":"10.1111/jeu.70068","DOIUrl":"10.1111/jeu.70068","url":null,"abstract":"<div>\u0000 \u0000 Antimicrobial resistance (AMR) is becoming a major problem in the treatment of infectious diseases, increasing the need for new antimicrobial agents. Although bacteria and fungi have been extensively explored in antimicrobial discovery, protozoa remain comparatively underexplored despite their biological diversity and metabolic capabilities. Recent studies have reported that several protozoan species, including Acanthamoeba, Dictyostelium, Naegleria, and Entamoeba, are capable of exhibiting antimicrobial activity, particularly against multidrug-resistant (MDR) bacteria and fungi. These activities have been associated with a range of bioactive molecules, such as pore-forming peptides, proteases, and secondary metabolites produced by protozoa. The reported antimicrobial effects involve multiple mechanisms, including disruption of membrane integrity, induction of oxidative stress, and inhibition of biofilm formation. Despite these observations, research on protozoa-derived antimicrobial compounds remains limited due to challenges related to molecular characterization, large-scale cultivation, and the availability of in vivo data. This review summarizes current knowledge on bioactive molecules produced by protozoa, with emphasis on their ecological origins, mechanisms of action, and potential relevance to antimicrobial drug discovery. These findings suggest that protozoa-derived bioactive compounds may contribute to the ongoing search for novel and natural antimicrobial agents and may represent a promising complementary resource in efforts to combat antimicrobial resistance.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

International Symposium on Ciliate Biology (ISCB 2025) and Interdisciplinary Research 国际纤毛虫生物学研讨会（ISCB 2025）和跨学科研究

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-02-06 DOI: 10.1111/jeu.70066

Ravi Toteja, Komal Kamra, Rup Lal, Santosh Kumar, Utkarsh Sood, Princy Hira, Alan Warren, Harpreet Kaur, Swati Maurya, Sandeep Antil, Jyoti Dagar, Seema Makhija

{"title":"International Symposium on Ciliate Biology (ISCB 2025) and Interdisciplinary Research","authors":"Ravi Toteja, Komal Kamra, Rup Lal, Santosh Kumar, Utkarsh Sood, Princy Hira, Alan Warren, Harpreet Kaur, Swati Maurya, Sandeep Antil, Jyoti Dagar, Seema Makhija","doi":"10.1111/jeu.70066","DOIUrl":"10.1111/jeu.70066","url":null,"abstract":"<div>\u0000 \u0000 The International Symposium on Ciliate Biology (ISCB 2025) was held at Acharya Narendra Dev College, University of Delhi. The symposium brought together leading experts from around the world. Preceded by hands-on workshops in taxonomy and genomics, the symposium offered a dynamic platform for knowledge exchange, featuring plenary lectures, invited talks, oral presentations, and posters on topics spanning biodiversity, taxonomy, cell biology, model systems, health, toxicology, and science communication. Sessions highlighted ciliates' ecological significance—from climate change monitoring to their roles as bioindicators and agents of environmental restoration. Cutting-edge research showcased molecular, cellular, and integrative approaches, including phylogenomic analyses, innovative uses of ciliates in pollution detection, vector control, and explored practical applications of ciliates in health and agriculture. The event drew approximately 300 participants, with strong international representation, fostering new collaborations and inspiring future research. Recommendations from the symposia advocate for enhanced interdisciplinary efforts, expanded molecular tools, frugal science innovations, and greater public science communication. The ISCB 2025 concluded with a cultural program and a General Body Meeting of the Indian Society of Ciliate Biology (ISoCB) outlining strategic directions for ISoCB, including global membership and triennial symposia. The event underlined the pivotal role of ciliates in biological research, environmental stewardship, and education.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146129793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Features of the Scuticociliate Pathogen Philaster sp. Isolate FWC2 That Causes Sea Urchin Mass Mortality 导致海胆大量死亡的囊藻致病菌Philaster sp. FWC2的遗传特征。

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-02-02 DOI: 10.1111/jeu.70065

Shen Jean Lim, Mya Breitbart

引用次数: 0

A Practical Approach to Study Uncultivated Protists Using Single-Cell Techniques for Electron Microscopy 利用电子显微镜单细胞技术研究原生生物的实用方法。

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-02-02 DOI: 10.1111/jeu.70061

Maia V. Palka, Kevin C. Wakeman, Eunji Park, Gordon Lax, Brian S. Leander

{"title":"A Practical Approach to Study Uncultivated Protists Using Single-Cell Techniques for Electron Microscopy","authors":"Maia V. Palka, Kevin C. Wakeman, Eunji Park, Gordon Lax, Brian S. Leander","doi":"10.1111/jeu.70061","DOIUrl":"10.1111/jeu.70061","url":null,"abstract":"Protists represent a significant portion of eukaryotic diversity with a wide range of ecological roles, lifestyles, and diverse morphological traits. Despite their widespread importance in ecological systems and their potential as model organisms, most protist lineages remain poorly characterized, in part due to their small size and the fact that many lineages remain uncultivated. High-resolution microscopy techniques like transmission and scanning electron microscopy (TEM and SEM) are powerful tools for studying protists, but the application of these techniques has significant limitations when applied to uncultivated species, largely due to the absence of reproducible methodologies tailored to studying single cells with small sample sizes. We present a robust protocol for preparing individual eukaryotic cells for TEM and SEM that addresses these limitations, can be implemented in the field, and uses inexpensive and easily obtainable materials. Our method minimizes cell loss during sample preparation for TEM and SEM, and it enables the tracking of single cells through TEM preparation to ensure cells can be localized and oriented appropriately for ultramicrotome sectioning. This protocol expands the feasibility of ultrastructural studies on uncultivated protist lineages and aims to make high-resolution microscopy more accessible for the broader community of protistologists who study uncultivated taxa.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12863111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Microbiome Within a Microbe: Rethinking Blastocystis Biology 微生物中的微生物组：对囊胚生物学的重新思考。

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-01-26 DOI: 10.1111/jeu.70056

Daisy Shaw, Eleni Gentekaki, Anastasios D. Tsaousis

{"title":"The Microbiome Within a Microbe: Rethinking Blastocystis Biology","authors":"Daisy Shaw, Eleni Gentekaki, Anastasios D. Tsaousis","doi":"10.1111/jeu.70056","DOIUrl":"10.1111/jeu.70056","url":null,"abstract":"Blastocystis spp., one of the most prevalent microeukaryotes in the human gut, has long puzzled researchers with its ambiguous role in health and disease. Decades-old microscopy studies reported bacterial- and viral-like particles within Blastocystis spp. cells, but these findings have been mainly overlooked. Comparable associations in other protozoa, such as those between Trichomonas vaginalis and Mycoplasma, as well as protozoan–virus interactions, are known to influence metabolism, immune evasion, and ecological fitness. Here, we revisit these neglected observations in Blastocystis spp., framing them within the holobiont concept and proposing that this protist may host its own microbial consortium. We also propose potential mechanisms, ecological implications, and modern experimental strategies—from organ-on-a-chip to single-cell multi-omics—to rigorously test this hypothesis. Recognizing Blastocystis spp. as a possible “microbiome within a microbe” could transform our understanding of its biology and its place in gut microbial ecology.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Romantic Discovery of Radiolaria in the Ocean 海洋中放射虫的浪漫发现。

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-01-18 DOI: 10.1111/jeu.70062

Johan Decelle

{"title":"The Romantic Discovery of Radiolaria in the Ocean","authors":"Johan Decelle","doi":"10.1111/jeu.70062","DOIUrl":"10.1111/jeu.70062","url":null,"abstract":"Radiolaria are unicellular marine organisms (protists) that have been drifting in oceanic plankton for hundreds of millions of years. These mineral architects can build extraordinarily complex skeletons, which fascinated and puzzled naturalists observing water samples through rudimentary microscopes. In the 19th century, the discovery and study of Radiolaria are associated with scientific voyages and human adventures. Naturalists who studied medicine and anatomy in European universities were captivated by the morphology of Radiolaria and expressed a profound wanderlust to collect them in the ocean. These intrepid and workaholic adventurers devoted their restless lives to studying microscopic life, while also actively engaging in teaching and sharing their observations and hypotheses with students. This article aims to retrace the discovery of Radiolaria through the lives of prominent naturalists and marine biologists, primarily Christian Gottfried Ehrenberg, Thomas Henry Huxley, Johannes Müller, and Ernst Haeckel. It also highlights the intellectual and geographic influences that shaped their research, including figures such as Johannes Wolfgang von Goethe and Alexander von Humboldt, as well as places like Jena, Helgoland, Villefranche-sur-Mer and Italy, which served as sampling locations and sources of romantic and artistic inspiration. Pioneering work on Radiolaria played a central role in shaping several emerging concepts (e.g., cell theory, individuality) and fields (e.g., taxonomy, evolution of morphology, symbiosis). The discovery of Radiolaria therefore reveals that even the most elusive marine microorganisms can deeply transform our understanding of life.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12813588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host Phosphorus Limitation Triggers Trade-Off Between Stoichiometric Homeostasis and Production in the Micro-Parasite Amoebophrya ceratii 寄主磷限制触发微寄生虫角状变形虫体内化学计量平衡和生产之间的权衡。

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-01-14 DOI: 10.1111/jeu.70060

Irene Romero Rodríguez, Cécile Jauzein, Emilie Rabiller, Sophie Schmitt, Florian Caradec, Mickael Le Gac, Claire Labry

{"title":"Host Phosphorus Limitation Triggers Trade-Off Between Stoichiometric Homeostasis and Production in the Micro-Parasite Amoebophrya ceratii","authors":"Irene Romero Rodríguez, Cécile Jauzein, Emilie Rabiller, Sophie Schmitt, Florian Caradec, Mickael Le Gac, Claire Labry","doi":"10.1111/jeu.70060","DOIUrl":"10.1111/jeu.70060","url":null,"abstract":"<div>\u0000 \u0000 Phosphorus (P) is a chemical element essential for life that can drive dynamics of microorganisms in marine environments. Nutrient acquisition by parasitic organisms is mainly done through infection. In the case of micro-parasites, how host diet limitations affect parasite stoichiometry and parasite–host interaction has not been thoroughly studied yet. This study focused on the free-living stage of Amoebophrya ceratii, a micro-parasite (Syndiniales, Dinoflagellates) of marine dinoflagellates. It aims to examine how host P limitation prior to infection affects parasite stoichiometry and infection cycle. To this end, Scrippsiella acuminata was used as a host and cultivated under P-replete or P-limiting conditions. Infection and parasite production were monitored, and elemental quotas and stoichiometry of the freshly produced dinospores were determined. In P-replete conditions, elemental quotas and ratios of the parasites were similar to their host, showing a strong need for P. While the host's stoichiometry was greatly impacted by P limitation, parasites were able to maintain stoichiometric homeostasis and elemental cell quotas. Finally, host P limitation mainly affected the dinospore production, exhibiting a trade-off between dinospores quotas, stoichiometric homeostasis, and production. These findings have implications for host–parasite interactions and biogeochemical cycling in coastal waters.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Methods to Characterize the Microbiota of Myxomycete Plasmodia 黏菌疟原虫菌群鉴定方法的比较。

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-12-17 DOI: 10.1111/jeu.70058

Juan Pablo Molina-Viramontes, Elizabeth Selene Gómez-Acata, Stephanie Hereira-Pacheco, Arturo Estrada-Torres, Yendi E. Navarro-Noya

引用次数: 0

GPI-Anchored Proteomes and Their Biochemical Pathway in Alveolates: Evolutionary Insights and Heterologous Expression in Tetrahymena Thermophila gpi锚定的蛋白质组及其在肺泡中的生化途径：嗜热四膜虫的进化见解和异源表达

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-12-08 DOI: 10.1111/jeu.70055

Gervasio Puca, María G. Montes, Juan Orlowski, Mariela Lujan Tomazic, Daniel H. González Maglio, Carolina de la Fuente, Mónica Florin-Christensen, Leonhard Schnittger, Alejandro D. Nusblat

{"title":"GPI-Anchored Proteomes and Their Biochemical Pathway in Alveolates: Evolutionary Insights and Heterologous Expression in Tetrahymena Thermophila","authors":"Gervasio Puca, María G. Montes, Juan Orlowski, Mariela Lujan Tomazic, Daniel H. González Maglio, Carolina de la Fuente, Mónica Florin-Christensen, Leonhard Schnittger, Alejandro D. Nusblat","doi":"10.1111/jeu.70055","DOIUrl":"https://doi.org/10.1111/jeu.70055","url":null,"abstract":"<div>\u0000 \u0000 Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) play essential roles in eukaryotic cell biology, yet their evolutionary diversity across alveolates remains poorly understood. Here, we conducted a comparative genomic analysis of GPI biosynthetic pathways and GPI-AP repertoires across representative species of Ciliophora, Apicomplexa, and Dinoflagellata. Our findings reveal lineage-specific patterns of gene loss, expansion, and functional divergence, with ciliates exhibiting reduced canonical enzyme sets but expanded trafficking components. Using a benchmarked pipeline of GPI-AP predictors combined with signal peptide detection, we identified candidate GPI-anchored proteins and performed functional annotation to determine enriched Gene Ontology terms. To complement the bioinformatic analyses, we expressed the apicomplexan GPI-anchored antigen GPI4 from Babesia bovis in Tetrahymena thermophila. This approach extends previous efforts that used Tetrahymena as a heterologous expression system for parasite surface antigens. The recombinant protein localized to ciliary membranes and showed features consistent with proper processing. This study highlights the evolutionary plasticity of GPI systems in alveolates and reinforces the utility of Tetrahymena as a eukaryotic platform for the production and analysis of GPI-anchored proteins with potential diagnostic or immunoprophylactic applications.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of Superoxide Dismutase and Its Putative Relationship With Drug Resistance in Eimeria Tenella 柔嫩艾美球虫超氧化物歧化酶的分子特征及其与耐药的关系

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-12-08 DOI: 10.1111/jeu.70057

Xinrui Xie, Yu Yu, Guilin Liu, Qiping Zhao, Shunhai Zhu, Jinwen Wang, Jiayu Bai, Wenqi Han, Hui Dong, Hongyu Han

{"title":"Molecular Characterization of Superoxide Dismutase and Its Putative Relationship With Drug Resistance in Eimeria Tenella","authors":"Xinrui Xie, Yu Yu, Guilin Liu, Qiping Zhao, Shunhai Zhu, Jinwen Wang, Jiayu Bai, Wenqi Han, Hui Dong, Hongyu Han","doi":"10.1111/jeu.70057","DOIUrl":"https://doi.org/10.1111/jeu.70057","url":null,"abstract":"<div>\u0000 \u0000 Avian coccidiosis, an intestinal parasitic disease caused by Eimeria spp., remains one of the most economically significant diseases in poultry. Although anticoccidial drugs have been effective in controlling coccidiosis, the emergence of drug resistance in Eimeria poses a persistent challenge to disease management. Our previous study revealed differential expression of superoxide dismutase (EtSOD) between drug-sensitive (DS) and drug-resistant Eimeria tenella strains. In this study, qPCR and western blot analyses demonstrated that EtSOD expression was significantly upregulated in diclazuril-resistant (DZR) and maduramicin-resistant (MRR) strains compared to the DS strain, with a progressive increase corresponding to higher drug concentrations. Enzyme activity assays further confirmed that SOD activity was elevated in both resistant strains. Additionally, qPCR results indicated higher EtSOD mRNA levels in second-generation merozoites and unsporulated oocysts than in sporulated oocysts and sporozoites. Indirect immunofluorescence revealed that fluorescence intensity increased during parasite development within host cells. Notably, EtSOD was predominantly localized on the surface of sporozoites and merozoites, as well as on the parasitophorous vacuole membrane (PVM) post-invasion of DF-1 cells, suggesting its potential role in host–parasite interactions and immune evasion. Collectively, these findings imply that EtSOD may contribute to drug resistance and facilitate parasite growth and development within host cells.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0