Journal of Eukaryotic Microbiology最新文献_第9页

Molecular and morphological characterization of four new ancyromonad genera and proposal for an updated taxonomy of the Ancyromonadida 四新弓形虫属的分子和形态特征及弓形虫纲分类的更新建议。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-08-22 DOI: 10.1111/jeu.12997

Naoji Yubuki, Guifré Torruella, Luis Javier Galindo, Aaron A. Heiss, Maria Cristina Ciobanu, Takashi Shiratori, Ken-ichiro Ishida, Jazmin Blaz, Eunsoo Kim, David Moreira, Purificación López-García, Laura Eme

{"title":"Molecular and morphological characterization of four new ancyromonad genera and proposal for an updated taxonomy of the Ancyromonadida","authors":"Naoji Yubuki, Guifré Torruella, Luis Javier Galindo, Aaron A. Heiss, Maria Cristina Ciobanu, Takashi Shiratori, Ken-ichiro Ishida, Jazmin Blaz, Eunsoo Kim, David Moreira, Purificación López-García, Laura Eme","doi":"10.1111/jeu.12997","DOIUrl":"10.1111/jeu.12997","url":null,"abstract":"Ancyromonads are small biflagellated protists with a bean-shaped morphology. They are cosmopolitan in marine, freshwater, and soil environments, where they attach to surfaces while feeding on bacteria. These poorly known grazers stand out by their uncertain phylogenetic position in the tree of eukaryotes, forming a deep-branching “orphan” lineage that is considered key to a better understanding of the early evolution of eukaryotes. Despite their ecological and evolutionary interest, only limited knowledge exists about their true diversity. Here, we aimed to characterize ancyromonads better by integrating environmental surveys with behavioral observation and description of cell morphology, for which sample isolation and culturing are indispensable. We studied 18 ancyromonad strains, including 14 new isolates and seven new species. We described three new and genetically divergent genera: Caraotamonas, Nyramonas, and Olneymonas, together encompassing four species. The remaining three new species belong to the already-known genera Fabomonas and Ancyromonas. We also raised Striomonas, formerly a subgenus of Nutomonas, to full genus status, on morphological and phylogenetic grounds. We studied the morphology of diverse ancyromonads under light and electron microscopy and carried out molecular phylogenetic analyses, also including 18S rRNA gene sequences from several environmental surveys. Based on these analyses, we have updated the taxonomy of Ancyromonadida.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10396105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of protists to nitrogen addition, arbuscular mycorrhizal fungi manipulation, and mesofauna reduction in a tropical montane rainforest in southern Ecuador 厄瓜多尔南部热带山地雨林原生生物对氮添加、丛枝菌根真菌控制和中系动物减少的响应。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-08-14 DOI: 10.1111/jeu.12996

Garvin Schulz, Tessa Camenzind, Laura M. Sánchez-Galindo, Dominik Schneider, Stefan Scheu, Valentyna Krashevska

{"title":"Response of protists to nitrogen addition, arbuscular mycorrhizal fungi manipulation, and mesofauna reduction in a tropical montane rainforest in southern Ecuador","authors":"Garvin Schulz, Tessa Camenzind, Laura M. Sánchez-Galindo, Dominik Schneider, Stefan Scheu, Valentyna Krashevska","doi":"10.1111/jeu.12996","DOIUrl":"10.1111/jeu.12996","url":null,"abstract":"The tropical Andes are a species-rich and nitrogen-limited system, susceptible to increased nitrogen (N) inputs from the atmosphere. However, our understanding of the impacts of increased N input on belowground systems, in particular on protists and their role in nutrient cycling, remains limited. We explored how increased N affects protists in tropical montane rainforests in Ecuador using high-throughput sequencing (HTS) of environmental DNA from two litter layers. In addition, we manipulated the amount of arbuscular mycorrhizal fungi (AMF) and mesofauna, both playing a significant role in N cycling and interacting in complex ways with protist communities. We found that N strongly affected protist community composition in both layers, while mesofauna reduction had a stronger effect on the lower layer. Changes in concentration of the AMF marker lipid had little effect on protists. In both layers, the addition of N increased phagotrophs and animal parasites and decreased plant parasites, while mixotrophs decreased in the upper layer but increased in the lower layer. In the upper layer with higher AMF concentration, mixotrophs decreased, while in the lower layer, photoautotrophs increased and plant parasites decreased. With reduced mesofauna, phagotrophs increased and animal parasites decreased in both layers, while plant parasites increased only in the upper layer. The findings indicate that to understand the intricate response of protist communities to environmental changes, it is critical to thoroughly analyze these communities across litter and soil layers, and to include HTS.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9988439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form Trypanosoma brucei parasites aak1样:一种假定的假激酶，在布鲁氏锥虫寄生虫血液中的货物摄取中具有潜在的作用。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-08-07 DOI: 10.1111/jeu.12994

Jennifer A. Black, Christen M. Klinger, Leandro Lemgruber, Joel B. Dacks, Jeremy C. Mottram, Richard McCulloch

{"title":"AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form Trypanosoma brucei parasites","authors":"Jennifer A. Black, Christen M. Klinger, Leandro Lemgruber, Joel B. Dacks, Jeremy C. Mottram, Richard McCulloch","doi":"10.1111/jeu.12994","DOIUrl":"10.1111/jeu.12994","url":null,"abstract":"Selection and internalization of cargo via clathrin-mediated endocytosis requires adaptor protein complexes. One complex, AP-2, acts during cargo selection at the plasma membrane. African trypanosomes lack all components of the AP-2 complex, except for a recently identified orthologue of the AP-2-associated protein kinase 1, AAK1. In characterized eukaryotes, AAK1 phosphorylates the μ2 subunit of the AP-2 complex to enhance cargo recognition and uptake into clathrin-coated vesicles. Here, we show that kinetoplastids encode not one, but two AAK1 orthologues: one (AAK1L2) is absent from salivarian trypanosomes, while the other (AAK1L1) lacks important kinase-specific residues in a range of trypanosomes. These AAK1L1 and AAK1L2 novelties reinforce suggestions of functional divergence in endocytic uptake within salivarian trypanosomes. Despite this, we show that AAK1L1 null mutant Trypanosoma brucei, while viable, display slowed proliferation, morphological abnormalities including swelling of the flagellar pocket, and altered cargo uptake. In summary, our data suggest an unconventional role for a putative pseudokinase during endocytosis and/or vesicular trafficking in T. brucei, independent of AP-2.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10008180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and molecular characterization of a new member of the phylum Rhodelphidia 蛛形纲新成员的形态和分子特征。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-08-07 DOI: 10.1111/jeu.12995

Kristina I. Prokina, Denis V. Tikhonenkov, Purificación López-García, David Moreira

引用次数: 0

Seasonal dynamics of eukaryotic microbial diversity in hypersaline Tuz Lake characterized by 18S rDNA sequencing 基于18S rDNA测序的高盐湖真核微生物多样性季节动态

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-08-01 DOI: 10.1111/jeu.12993

Suzan Şahin Doğan, Aytaç Kocabaş

{"title":"Seasonal dynamics of eukaryotic microbial diversity in hypersaline Tuz Lake characterized by 18S rDNA sequencing","authors":"Suzan Şahin Doğan, Aytaç Kocabaş","doi":"10.1111/jeu.12993","DOIUrl":"10.1111/jeu.12993","url":null,"abstract":"Microbial diversity found in hypersaline ecosystems is structurally unique and essential in many microbiological and ecological processes. Tuz Lake, the second biggest lake in Türkiye, is a talassohaline (over 32% [w/v]) lake with near-neutral pH. The aim of study was to investigate the composition of the eukaryotic microbial community in Tuz Lake by 18S rDNA amplicon sequencing, as well as its relationship and change with environmental factors during 1-year period. Next-generation sequencing and bioinformatic analysis were applied to describe the eukaryotic microbial community in Tuz Lake. As a result of bioinformatics analysis, Archaeplastida (39%) and Stramenopiles, Alveolata, Rhizaria (SAR) (51%) were the most abundant taxa represented in the dataset. The Archaeplastida phylum showed a significant difference between winter and summer and higher abundance in summer in contrast to the SAR group, which represented higher abundance in winter. Genus level assessment showed that the most abundant genera were Navicula, Chlorophyta;unclassified_taxa, Dunaliella, Cladosporium, Paraphelidium, Scuticociliates;unclassified_taxa, and Chlamydomonadales;unclassified_taxa. Navicula abundance was significantly different and overwhelmingly dominant in winter. On the other hand, Cladosporium and Chlorophyta; unclassified_taxa represented a significant difference between seasons and high abundance in summer. Furthermore, Dunaliella populations were not detected in midsummer and early fall when the temperature increased and water volume in the lake decreased.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9974730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Unexpected ubiquity of heart-shaped scale morphotype in Centroplasthelida (Haptista): Ancestral trait or multiple acquisitions? 心形鳞片形态在中心体动物(Haptista)中出乎意料的普遍存在:祖先特征还是多重获得?

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-07-14 DOI: 10.1111/jeu.12992

Elena A. Gerasimova, Yulia V. Mindolina, Denis V. Tikhonenkov, Vladimir Y. Kataev, Alexander S. Balkin, Kirill V. Mikhailov, Dmitry G. Zagumyonnyi, Andrey O. Plotnikov, Vasily V. Zlatogursky

{"title":"Unexpected ubiquity of heart-shaped scale morphotype in Centroplasthelida (Haptista): Ancestral trait or multiple acquisitions?","authors":"Elena A. Gerasimova, Yulia V. Mindolina, Denis V. Tikhonenkov, Vladimir Y. Kataev, Alexander S. Balkin, Kirill V. Mikhailov, Dmitry G. Zagumyonnyi, Andrey O. Plotnikov, Vasily V. Zlatogursky","doi":"10.1111/jeu.12992","DOIUrl":"10.1111/jeu.12992","url":null,"abstract":"Centrohelids (Haptista: Centroplasthelida) are axopodial protists with a remarkable diversity of external siliceous scale morphologies. It is believed that the last common ancestor of centrohelids had a double layer of siliceous scales composed of plate scales closer to a cell surface and spine scales radiating outwards. The characteristic morphotype of spine scales with a heart-shaped base was once believed to be a unique feature of the genus Choanocystis, as it was defined by Siemensma and Roijackers (1988). Further research revealed that this morphology is present in different and sometimes distantly related lineages: Ozanamiidae, Meringosphaeridae, and Marophryidae. Here, we report the fourth clade, Pterocystidae, which is also revealed to contain representatives having this phenotype. Cernunnos gen. nov. is erected here to place Cernunnos uralica sp. nov., Cernunnos arctica sp. nov., Cernunnos america sp. nov., and Cernunnos antarctica Tikhonenkov et Mylnikov, 2010, Gerasimova comb. nov. C. uralica was studied with scanning electron microscopy and SSU rDNA sequencing. Molecular phylogenetic analysis placed it into marine environmental clade P within Pterocystida. The ubiquity of spine scales with heart-shaped bases could be an example of parallel evolution, but taking into account the considerable similarity it is likely an ancestral trait, acquired from the last common ancestor of centrohelids.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9779599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interpreting phylogenetic placements for taxonomic assignment of environmental DNA 环境DNA分类分配的系统发育定位解释

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-07-13 DOI: 10.1111/jeu.12990

Isabelle Ewers, Lubomír Rajter, Lucas Czech, Frédéric Mahé, Alexandros Stamatakis, Micah Dunthorn

{"title":"Interpreting phylogenetic placements for taxonomic assignment of environmental DNA","authors":"Isabelle Ewers, Lubomír Rajter, Lucas Czech, Frédéric Mahé, Alexandros Stamatakis, Micah Dunthorn","doi":"10.1111/jeu.12990","DOIUrl":"10.1111/jeu.12990","url":null,"abstract":"Taxonomic assignment of operational taxonomic units (OTUs) is an important bioinformatics step in analyzing environmental sequencing data. Pairwise alignment and phylogenetic‐placement methods represent two alternative approaches to taxonomic assignments, but their results can differ. Here we used available colpodean ciliate OTUs from forest soils to compare the taxonomic assignments of VSEARCH (which performs pairwise alignments) and EPA‐ng (which performs phylogenetic placements). We showed that when there are differences in taxonomic assignments between pairwise alignments and phylogenetic placements at the subtaxon level, there is a low pairwise similarity of the OTUs to the reference database. We then showcase how the output of EPA‐ng can be further evaluated using GAPPA to assess the taxonomic assignments when there exist multiple equally likely placements of an OTU, by taking into account the sum over the likelihood weights of the OTU placements within a subtaxon, and the branch distances between equally likely placement locations. We also inferred the evolutionary and ecological characteristics of the colpodean OTUs using their placements within subtaxa. This study demonstrates how to fully analyze the output of EPA‐ng, by using GAPPA in conjunction with knowledge of the taxonomic diversity of the clade of interest.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.12990","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10181404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular phylogenetics of sessile Dolium sedentarium, a petalomonad euglenid 叶柄蓼属无柄蓼的分子系统发育

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-07-09 DOI: 10.1111/jeu.12991

Gordon Lax, Patrick J. Keeling

{"title":"Molecular phylogenetics of sessile Dolium sedentarium, a petalomonad euglenid","authors":"Gordon Lax, Patrick J. Keeling","doi":"10.1111/jeu.12991","DOIUrl":"10.1111/jeu.12991","url":null,"abstract":"The euglenids are a species-rich group of flagellates with varying modes of nutrition that can be found in diverse habitats. Phagotrophic members of this group gave rise to phototrophs and hold the key to understanding the evolution of euglenids as a whole, including the evolution of complex morphological characters like the euglenid pellicle. Yet to understand the evolution of these characters, a comprehensive sampling of molecular data is needed to correlate morphological and molecular data, and to estimate a basic phylogenetic backbone of the group. While the availability of SSU rDNA and, more recently, multigene data from phagotrophic euglenids has improved, several “orphan” taxa remain without any molecular data whatsoever. Dolium sedentarium is one such taxon: It is a rarely-observed phagotrophic euglenid that inhabits tropical benthic environments and is one of few known sessile euglenids. Based on morphological characters, it has been thought of as part of the earliest branch of euglenids, the Petalomonadida. We report the first molecular sequencing data for Dolium using single-cell transcriptomics, adding another small piece in the puzzle of euglenid evolution. Both SSU rDNA and multigene phylogenies confirm it as a solitary branch within Petalomonadida.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.12991","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10128218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fine structure and molecular characterization of two new parabasalid species that naturally colonize laboratory mice, Tritrichomonas musculus and Tritrichomonas casperi 实验小鼠自然定植的两种新的副拟虫种——肌肉毛滴虫和casperi毛滴虫的精细结构和分子特征。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-06-10 DOI: 10.1111/jeu.12989

Leila Tuzlak, Eliza V. C. Alves-Ferreira, Cindi L. Schwartz, Andrea Kennard, Jacqueline M. Leung, Christina Shehata, Michael E. Grigg

{"title":"Fine structure and molecular characterization of two new parabasalid species that naturally colonize laboratory mice, Tritrichomonas musculus and Tritrichomonas casperi","authors":"Leila Tuzlak, Eliza V. C. Alves-Ferreira, Cindi L. Schwartz, Andrea Kennard, Jacqueline M. Leung, Christina Shehata, Michael E. Grigg","doi":"10.1111/jeu.12989","DOIUrl":"10.1111/jeu.12989","url":null,"abstract":"Tritrichomonas muris is a common flagellated protist isolated from the cecum of wild rodents. This commensal protist has been shown previously to alter immune phenotypes in laboratory mice. Other trichomonads, referred to as Tritrichomonas musculis and Tritrichomonas rainier, also naturally colonize laboratory mice and cause immune alterations. This report formally describes two new trichomonads, Tritrichomonas musculus n. sp., and Tritrichomonas casperi n. sp., at the ultrastructural and molecular level. These two protists were isolated from laboratory mice and were differentiated by their size and the structure of their undulating membrane and posterior flagellum. Analysis at the 18S rRNA and trans-ITS genetic loci supported their designation as distinct species, related to T. muris. To assess the true extent of parabasalid diversity infecting laboratory mice, 135 mice bred at the National Institutes of Health (NIH) were screened using pan-parabasalid primers that amplify the trans-ITS region. Forty-four percent of mice were positive for parabasalids, encompassing a total of eight distinct sequence types. Tritrichomonas casperi and Trichomitus-like protists were dominant. T. musculus and T. rainier were also detected, but T. muris was not. Our work establishes a previously underappreciated diversity of commensal trichomonad flagellates that naturally colonize the enteric cavity of laboratory mice.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10261971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

True molecular phylogenetic position of the cockroach gut commensal Lophomonas blattarum (Lophomonadida, Parabasalia) 蟑螂肠道共生体blattarum (Lophomonadida, Parabasalia)的真分子系统发育定位

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-06-08 DOI: 10.1111/jeu.12988

LeAnn Nguyen, Stephen J. Taerum, Daniel E. Jasso-Selles, Claudio H. Slamovits, Jeffrey D. Silberman, Gillian H. Gile

引用次数: 1