Journal of Eukaryotic Microbiology最新文献

{"title":"Anaeramoeba pumila sp. nov. and Anaeramoeba sp. OCE22C represent two novel types of symbiosis of Anaeramoebae and prokaryotes","authors":"Magdaléna Pavlátová,&nbsp;Pavla Hanousková,&nbsp;Ivan Čepička","doi":"10.1111/jeu.13008","DOIUrl":"10.1111/jeu.13008","url":null,"abstract":"<p>Anaeramoebae is a recently described phylum of anaerobic, marine amoebae, and amoeboflagellates belonging to the Metamonada supergroup. So far, six species have been described based on light microscopic morphology and sequences of the SSU rRNA gene. Here we present three new strains of <i>Anaeramoeba</i> with a description of their morphology, ultrastructure, and phylogenetic position based on the analysis of SSU rRNA gene sequences. Two of the strains represent a new species, <i>Anaeramoeba pumila</i> sp. nov., that has the smallest cells of all known <i>Anaeramoeba</i> species, and one that represents a species from the newly recognized <i>Anaeramoeba flamelloides</i> complex. Anaeramoebae are known to have a syntrophic relationship with prokaryotes. Our strains display two novel, remarkable types of symbioses, previously unknown from Anaeramoebae.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71482149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly divergent morphology but a close molecular phylogenetic relationship between two little-known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species","authors":"Yong Chi,&nbsp;Danxu Tang,&nbsp;Jingtao Lei,&nbsp;Fan Wei,&nbsp;Saleh A. Al-Farraj,&nbsp;William A. Bourland,&nbsp;Zigui Chen","doi":"10.1111/jeu.13007","DOIUrl":"10.1111/jeu.13007","url":null,"abstract":"<p>Free-living litostomatean ciliates, prominent microeukaryote predators commonly encountered in freshwater and marine habitats, play vital roles in maintaining energy flow and nutrient cycles. Nevertheless, understanding their biodiversity and phylogenetic relationships remains challenging due to insufficient morphological information and molecular data. As a new contribution to this group, three haptorian ciliates, including two new species (<i>Actinobolina bivacuolata</i> sp. nov. and <i>Papillorhabdos foissneri</i> sp. nov.) and the insufficiently described type species, <i>Actinobolina radians</i>, were isolated from wetlands around Lake Weishan, China and investigated by a combination of living morphology, stained preparations, and 18S rRNA gene sequence data. An illustrated key of the valid species within the two genera is provided. In addition, we reveal the phylogenetic positions of these two genera for the first time. Although they differ in all key morphologic characters such as general appearance (ellipsoidal with numerous tentacles vs. cylindrical), extrusomes (stored in tentacles vs. anchored to pellicle), circumoral kinety (present vs. absent), composition of somatic kineties (kinetosome clusters vs. monokinetids), and number of dorsal brush rows (1 vs. 4), they both cluster in a fully supported clade in the phylogenetic tree, which indicates that the biodiversity and additional molecular markers of this group need further exploration.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54229275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell DNA from West Greenland marine sediments suggests presence of Protoperidinium tricingulatum in the Arctic","authors":"Tiia Luostarinen,&nbsp;Sofia Ribeiro,&nbsp;Heike H. Zimmermann,&nbsp;Anna B. Kvorning,&nbsp;Maija Heikkilä","doi":"10.1111/jeu.13005","DOIUrl":"10.1111/jeu.13005","url":null,"abstract":"<p>Spiny brown dinoflagellate cysts are commonly used as sea-ice indicators in the Arctic, but their biological affinities are not well known. We present the first indication of hitherto temperate <i>Protoperidinium tricingulatum</i> in the Arctic based on single-cell LSU rDNA sequencing from sediments of the Disko Bay-Vaigat Sound, West Greenland. The morphological similarity of the sequenced cyst morphotype to the sea-ice indicator <i>Islandinium</i>? <i>cezare</i> morphotype 1 is striking. The morphology of the isolated cysts, as well as those observed in the total cyst assemblage following standard palynological preparation, both resemble either <i>I.? cezare</i> morphotype 1 or <i>P. tricingulatum</i>, suggesting that the specimens may in fact be close morphological variants of the same species. In addition, nine LSU rDNA sequences were obtained from morphological variants assigned to <i>Islandinium minutum</i> s.l.: including both subspecies <i>minutum</i> and subspecies <i>barbatum</i>. The two subspecies could not be differentiated based on partial LSU rDNA sequencing. Overall, Arctic spiny brown dinoflagellate cyst species may be morphologically more diverse and taxonomically more complex than shown earlier and further genetic and morphological studies are needed. Importantly, the value of cysts as palaeoecological indicators depends on a sound understanding of their biological affinity and taxonomy.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50158085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paramecium (Oligohymenophorea, Ciliophora) diversity in Thailand sheds light on the genus biogeography and reveals new phylogenetic lineages","authors":"Maksim Melekhin,&nbsp;Alexey Potekhin,&nbsp;Eleni Gentekaki,&nbsp;Chitchai Chantangsi","doi":"10.1111/jeu.13004","DOIUrl":"10.1111/jeu.13004","url":null,"abstract":"<p><i>Paramecium</i> (Ciliophora, Oligohymenophorea) is a good model to study ciliate biogeography. Extensive sampling mainly in northern hemisphere has led to 16 valid morphological species description thus far. However, a majority of hard-to-reach regions, including South East Asia, are underinvestigated. Our study combined traditional morphological and molecular approaches to reveal the biodiversity of <i>Paramecium</i> in Thailand from more than 110 samples collected in 10 provinces. Representatives of seven morphological species were identified from our collection, including the rare species, such as <i>P. gigas</i> and <i>P. jenningsi</i>. Additionally, we detected five different sibling species of the <i>P. aurelia</i> complex, described a new cryptic species <i>P. hiwatashii</i> n. sp. phylogenetically related to <i>P. caudatum,</i> and discovered a potentially new genetic species of the <i>P. bursaria</i> species complex. We also documented a variety of bacterial cytoplasmic symbionts from at least nine monoclonal cultures of <i>Paramecium</i>.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41235804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kaonashia insperata gen. et sp. nov., a eukaryotrophic flagellate, represents a novel major lineage of heterotrophic stramenopiles","authors":"Elizabeth J. Weston,&nbsp;Yana Eglit,&nbsp;Alastair G. B. Simpson","doi":"10.1111/jeu.13003","DOIUrl":"10.1111/jeu.13003","url":null,"abstract":"<p>Eukaryotrophic protists are ecologically significant and possess characteristics key to understanding the evolution of eukaryotes; however, they remain poorly studied, due partly to the complexities of maintaining predator–prey cultures. <i>Kaonashia insperata</i>, gen. nov., et sp. nov., is a free-swimming biflagellated eukaryotroph with a conspicuous ventral groove, a trait observed in distantly related lineages across eukaryote diversity. Di-eukaryotic (predator–prey) cultures of <i>K</i>. <i>insperata</i> with three marine algae (<i>Isochrysis galbana</i>, <i>Guillardia theta</i>, and <i>Phaeodactylum tricornutum</i>) were established by single-cell isolation. Growth trials showed that the studied <i>K</i>. <i>insperata</i> clone grew particularly well on <i>G</i>. <i>theta</i>, reaching a peak abundance of 1.0 × 10⁵ ± 4.0 × 10⁴ cells ml⁻¹. Small-subunit ribosomal DNA phylogenies infer that <i>K</i>. <i>insperata</i> is a stramenopile with moderate support; however, it does not fall within any well-defined phylogenetic group, including environmental sequence clades (e.g. MASTs), and its specific placement remains unresolved. Electron microscopy shows traits consistent with stramenopile affinity, including mastigonemes on the anterior flagellum and tubular mitochondrial cristae. <i>Kaonashia insperata</i> may represent a novel major lineage within stramenopiles, and be important for understanding the evolutionary history of the group. While heterotrophic stramenopile flagellates are considered to be predominantly bacterivorous, eukaryotrophy may be relatively widespread amongst this assemblage.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41124172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichite features contribute to the revision of the genus Strombidium (Alveolata, Ciliophora, Spirotricha)","authors":"Sabine Agatha,&nbsp;Birgit Weißenbacher,&nbsp;Michael Kirschner,&nbsp;Maximilian H. Ganser","doi":"10.1111/jeu.13001","DOIUrl":"10.1111/jeu.13001","url":null,"abstract":"<p><i>Strombidium</i> is a species-rich genus of oligotrichid ciliates mainly inhabiting the marine pelagial. In molecular phylogenies, the genus emerged as non-monophyletic, and cladistic analyses suggest that it is largely characterized by plesiomorphies. A reliable split of the genus and the establishment of new genera necessitate, however, support by novel morphological and/or ultrastructural features. In the present study, the arrangement and ultrastructure of trichites are proposed as taxonomically relevant characters. <i>Strombidium biarmatum</i> Agatha et al., 2005 differs in the trichite pattern from the type species <i>Strombidium sulcatum</i> and most congeners. Aside from the trichites inserting anteriorly to the girdle kinety and generating the typical funnel-shaped complex in the posterior cell portion, the species displays additional trichites between the adoral membranelles even visible in live cells. Here, this exceptional trichite arrangement is detailed based on transmission electron microscopic investigations. In molecular phylogenies, <i>S. biarmatum</i> forms a monophylum with two congeners sharing its trichite arrangement. Therefore, the strombidiid genus <i>Heteropilum</i> nov. gen. is established with <i>S. biarmatum</i> as type species to also include <i>H. paracapitatum</i> (Song et al., 2015) nov. comb. and <i>H. basimorphum</i> (Martin &amp; Montagnes, 1993) nov. comb. Further differences discovered in the trichite ultrastructure support the organelles' taxonomic significance.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41176303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudovampyrella gen. nov.: A genus of Vampyrella-like protoplast extractors finds its place in the Leptophryidae","authors":"Andreas Suthaus,&nbsp;Sebastian Hess","doi":"10.1111/jeu.13002","DOIUrl":"10.1111/jeu.13002","url":null,"abstract":"<p>Vampyrellid amoebae are predatory protists, which consume a variety of eukaryotic prey and inhabit freshwater, marine and terrestrial ecosystems. Although they have been known for almost 150 years, much of their diversity lacks an in-depth characterization. To date, environmental sequencing data hint at several uncharacterized lineages, to which no phenotype is associated. Furthermore, there are numerous historically described species without any molecular information. This study reports on two new vampyrellid strains from moorlands, which extract the protoplasts of <i>Closterium</i> species (Zygnematophyceae). Our data on morphology, prey range specificity and feeding strategy reveal that the studied vampyrellids are very similar to the historically described <i>Vampyrella closterii</i>. However, phylogenetic analyses demonstrate that the two strains do not belong to the genus <i>Vampyrella</i> and, instead, form a distinct clade in the family Leptophryidae. Hence, we introduce a new genus of algivorous protoplast extractors, <i>Pseudovampyrella</i> gen. nov., with the species <i>P. closterii</i> (= <i>V. closterii</i>) and <i>P. minor</i>. Our findings indicate that the genetic diversity of morphologically described vampyrellid species might be hugely underrated.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene editing of putative cAMP and Ca2+-regulated proteins using an efficient cloning-free CRISPR/Cas9 system in Trypanosoma cruzi","authors":"Miguel A. Chiurillo,&nbsp;Milad Ahmed,&nbsp;César González,&nbsp;Aqsa Raja,&nbsp;Noelia Lander","doi":"10.1111/jeu.12999","DOIUrl":"https://doi.org/10.1111/jeu.12999","url":null,"abstract":"<p><i>Trypanosoma cruzi</i>, the agent of Chagas disease, must adapt to a diversity of environmental conditions that it faces during its life cycle. The adaptation to these changes is mediated by signaling pathways that coordinate the cellular responses to the new environmental settings. Cyclic AMP (cAMP) and Calcium (Ca²⁺) signaling pathways regulate critical cellular processes in this parasite, such as differentiation, osmoregulation, host cell invasion and cell bioenergetics. Although the use of CRISPR/Cas9 technology prompted reverse genetics approaches for functional analysis in <i>T</i>. <i>cruzi</i>, it is still necessary to expand the toolbox for genome editing in this parasite, as for example to perform multigene analysis. Here we used an efficient T7RNAP/Cas9 strategy to tag and delete three genes predicted to be involved in cAMP and Ca²⁺ signaling pathways: a putative Ca²⁺/calmodulin-dependent protein kinase (<i>CAMK</i>), Flagellar Member 6 (<i>FLAM6</i>) and Cyclic nucleotide-binding domain/C2 domain-containing protein (<i>CC2CP</i>). We endogenously tagged these three genes and determined the subcellular localization of the tagged proteins. Furthermore, the strategy used to knockout these genes allows us to presume that <i>TcCC2CP</i> is an essential gene in <i>T</i>. <i>cruzi</i> epimastigotes. Our results will open new venues for future research on the role of these proteins in <i>T</i>. <i>cruzi</i>.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"70 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71976560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure of Tritrichomonas foetus to sublethal doses of metronidazole induces a specific proinflammatory response in murine macrophages","authors":"Emanuel Ceballos-Góngora,&nbsp;Julio César Torres-Romero,&nbsp;Victor Ermilo Arana-Argáez,&nbsp;María Elizbeth Alvarez-Sánchez,&nbsp;Karla Acosta-Viana,&nbsp;Antonio Euan-Canto,&nbsp;Leidi Cristal Alvarez-Sánchez","doi":"10.1111/jeu.13000","DOIUrl":"10.1111/jeu.13000","url":null,"abstract":"<p><i>Tritrichomonas foetus</i> is a flagellated parasite that primarily infects the reproductive tissues of livestock, causing bovine trichomoniasis. The cytoplasmic membrane of <i>T</i>. <i>foetus</i> contains various compounds that contribute to adherence, colonization, and pathogenicity. Metronidazole (MTZ) is the main treatment for trichomoniasis, but the emergence of drug-resistant strains is a concern due to improper use and dosing. <i>T</i>. <i>foetus</i> infection induces inflammation, and macrophages are key players in the immune response. However, our understanding of the host's immune response to <i>T</i>. <i>foetus</i> is limited, and the specific mechanisms underlying these responses are not well understood. This study aimed to investigate the impact of <i>T</i>. <i>foetus</i> surface proteins from trophozoites cultured under different sublethal MTZ conditions (MTZ-treated <i>T</i>. <i>foetus</i> MPs) on macrophage activation. By analyzing cytokine levels and gene expression in murine macrophages, we demonstrated that MTZ-treated <i>T</i>. <i>foetus</i> MPs induce a specific proinflammatory response. MTZ-treated <i>T</i>. <i>foetus</i> MPs-exposed macrophages exhibited a higher NO and H₂O₂ production and overexpression of <i>iNOS</i> and <i>NOX-2</i> genes in comparison to untreated <i>T</i>. <i>foetus</i>. Additionally, MTZ-treated <i>T</i>. <i>foetus</i> MPs triggered a significant induction of the proinflammatory cytokines IL-1β, IL-6, TNF-α, and IFN-γ, as well as the overexpression of the <i>TLR4</i>, <i>MyD88</i>, and <i>NF-κB</i> genes on murine macrophages. The study aimed to unravel the immunological response and potential proinflammatory pathways involved in <i>T</i>. <i>foetus</i> infection and MTZ stress. Understanding the immune responses and mechanisms through which <i>T</i>. <i>foetus</i> surface proteins activate macrophages can contribute to the development of new therapeutic strategies for controlling bovine trichomoniasis.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10156796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Division of Blastocystis ST10 into three new subtypes: ST42-ST44","authors":"Monica Santin,&nbsp;Ana Figueiredo,&nbsp;Aleksey Molokin,&nbsp;Nadja S George,&nbsp;Pamela C. Köster,&nbsp;Alejandro Dashti,&nbsp;David González-Barrio,&nbsp;David Carmena,&nbsp;Jenny G. Maloney","doi":"10.1111/jeu.12998","DOIUrl":"10.1111/jeu.12998","url":null,"abstract":"<p>The <i>Blastocystis</i> subtype ST10 has been recognized to contain a great deal of diversity at the sequence level, potentially indicating the presence of multiple new STs within the clade. However, the data needed to validate these new STs were not available. To help resolve this diversity, full-length small subunit (<i>SSU</i>) rRNA gene reference sequences were generated using Oxford Nanopore MinION long-read sequencing from 21 samples representing multiple domestic and wild hosts and geographic regions and covering the sequence diversity previously described using fragments of the <i>SSU</i> rRNA gene. Phylogenetic and pairwise distance analyses were used to compare full-length sequences of the <i>SSU</i> rRNA gene generated in this study with all other valid STs of <i>Blastocystis</i>. We present data supporting the division of ST10/ST23 cluster into five subtypes, ST10, ST23, and three new subtypes with the proposed ST designations of ST42, ST43, and ST44. As the host range of <i>Blastocystis</i> continues to expand with new subtypes and new hosts being frequently identified, the reference sequences provided in this study will assist in accurate sequence classification and help to clarify the epidemiology of this common intestinal microeukaryote.</p>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10492466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}