Journal of Eukaryotic Microbiology最新文献_第7页

Grazing preferences of three species of amoebae on cyanobacteria and green algae 三种变形虫对蓝藻和绿藻的偏好。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-01-10 DOI: 10.1111/jeu.13018

Harold G. Weger, April K. Polasek, Derek M. Wright, Arun Damodaran, John Stavrinides

{"title":"Grazing preferences of three species of amoebae on cyanobacteria and green algae","authors":"Harold G. Weger, April K. Polasek, Derek M. Wright, Arun Damodaran, John Stavrinides","doi":"10.1111/jeu.13018","DOIUrl":"10.1111/jeu.13018","url":null,"abstract":"Twenty species/isolates of cyanobacteria and green algae were isolated from cyanobacterial bloom samples in lakes associated with the upper Qu'Appelle River drainage system in southern Saskatchewan, Canada. Three amoebae species (Cochliopodium sp., Vannella sp. and Vermamoeba vermiformis) were also isolated from one of these samples, and were subjected to grazing assays to determine which species of cyanobacteria or algae could potentially serve as a food source. Amoeba grazing rates were quantified based on the diameter of the plaque after 12 days on agar plate assays, and by estimation of the amoeba population growth rate from the rate of increase of plaque area. The common cyanobacterial bloom-formers Dolichospermum sp. and Aphanizomenon flos-aquae supported high growth rates for all three amoebae, while green algae, with the exception of one green alga/amoeba combination, did not support growth of the tested amoebae. Many of the cyanobacterial and algal isolates that did not support amoebae growth were ingested, suggesting that ingestion did not determine grazing success. Overall, while the cyanobacteria Dolichospermum sp. and Aphanizomenon flos-aquae were suitable food sources for the amoebae, the other cyanobacteria were grazed in an unpredictable manner, with some species/strains grazed by some amoebae and some species not grazed at all.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139403044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The function of the feeding groove of ‘typical excavate’ flagellates 典型挖掘型 "鞭毛虫摄食槽的功能

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-18 DOI: 10.1111/jeu.13016

Sei Suzuki-Tellier, Thomas Kiørboe, Alastair G. B. Simpson

{"title":"The function of the feeding groove of ‘typical excavate’ flagellates","authors":"Sei Suzuki-Tellier, Thomas Kiørboe, Alastair G. B. Simpson","doi":"10.1111/jeu.13016","DOIUrl":"10.1111/jeu.13016","url":null,"abstract":"Phagotrophic flagellates are the main consumers of bacteria and picophytoplankton. Despite their ecological significance in the ‘microbial loop’, many of their predation mechanisms remain unclear. ‘Typical excavates’ bear a ventral groove, where prey is captured for ingestion. The consequences of feeding through a ‘semi-rigid’ furrow on the prey size range have not been explored. An unidentified moving element called ‘the wave’ that sweeps along the bottom of the groove toward the site of phagocytosis has been observed in a few species; its function is unclear. We investigated the presence, behavior, and function of the wave in four species from the three excavate clades (Discoba, Metamonada, and Malawimonadida) and found it present in all studied cases, suggesting the potential homology of this feature across all three groups. The wave displayed a species-specific behavior and was crucial for phagocytosis. The morphology of the feeding groove had an upper-prey size limit for successful prey captures, but smaller particles were not constrained. Additionally, the ingestion efficiencies were species dependent. By jointly studying these feeding traits, we speculate on adaptations to differences in food availability to better understand their ecological functions.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138716896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The second most abundant dinophyte in the ponds of a botanical garden is a species new to science 植物园池塘中数量第二多的双子叶植物是科学界的新物种

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-11 DOI: 10.1111/jeu.13015

Anna Müller, Marina Stark, Sophia Schottenhammel, Uwe John, Juliana Chacón, Andreas Klingl, Victoria Julia Christine Holzer, Marika Schöffer, Marc Gottschling

{"title":"The second most abundant dinophyte in the ponds of a botanical garden is a species new to science","authors":"Anna Müller, Marina Stark, Sophia Schottenhammel, Uwe John, Juliana Chacón, Andreas Klingl, Victoria Julia Christine Holzer, Marika Schöffer, Marc Gottschling","doi":"10.1111/jeu.13015","DOIUrl":"10.1111/jeu.13015","url":null,"abstract":"In the microscopy realm, a large body of dark biodiversity still awaits to be uncovered. Unarmoured dinophytes are particularly neglected here, as they only present inconspicuous traits. In a remote German locality, we collected cells, from which a monoclonal strain was established, to study morphology using light and electron microscopy and to gain DNA sequences from the rRNA operon. In parallel, we detected unicellular eukaryotes in ponds of the Botanical Garden Munich-Nymphenburg by DNA-metabarcoding (V4 region of the 18S rRNA gene), weekly sampled over the course of a year. Strain GeoK*077 turned out to be a new species of Borghiella with a distinct position in molecular phylogenetics and characteristic coccoid cells of ovoid shape as the most important diagnostic trait. Borghiella ovum, sp. nov., was also present in artificial ponds of the Botanical Garden and was the second most abundant dinophyte detected in the samples. More specifically, Borghiella ovum, sp. nov., shows a clear seasonality, with high frequency during winter months and complete absence during summer months. The study underlines the necessity to assess the biodiversity, particularly of the microscopy realm more ambitiously, if even common species such as formerly Borghiella ovum are yet unknown to science.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs 镰刀形艾美耳菌细胞外囊泡表达宿主细胞lncRNA的差异

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-10 DOI: 10.1111/jeu.13009

Joshua S. Olajide, Zigang Qu, Shunli Yang, Bin Yang, Xiao Xu, Jing Wang, Jianping Cai

{"title":"Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs","authors":"Joshua S. Olajide, Zigang Qu, Shunli Yang, Bin Yang, Xiao Xu, Jing Wang, Jianping Cai","doi":"10.1111/jeu.13009","DOIUrl":"10.1111/jeu.13009","url":null,"abstract":"Long noncoding RNAs (lncRNAs) are regulatory transcripts during protozoan infections in the host intestinal epithelial cells (IECs). Apicomplexan Eimeria falciformis sporozoite extracellular vesicles (EVs) contain virulence factors that modulate host IECs pro-inflammatory genes and immune responses. In this study, E. falciformis sporozoites were made to interact with inactivated host cells, and the parasite EVs were separated from total secretome by ultracentrifugation and purified on density gradient medium. Dose-dependent bio-activity of E. falciformis EVs was investigated by RNA sequencing, functional annotation and quantitative PCR. It was found that E. falciformis EVs induced mRNA, circRNA, and lncRNA expressions in mouse IECs. Of 38, 217 lncRNAs assembled, 157 and 152 were upwardly and downwardly expressed respectively. Differentially expressed lncRNAs were associated with cytokines, pyroptosis, and immune signaling pathways including FoxO, NF-κB, MAPK, and TGF-β. In essence, E. falciformis EVs altered host cell RNA expressions during the interaction with host IECs. Also, differentially expressed lncRNAs are potential diagnostic transcripts during Eimeria infections.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to the article “On the phylogenetic position of Raphidocystis pallida with some notes on its life cycle” 更正。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-08 DOI: 10.1111/jeu.13011

引用次数: 0

In memoriam: Thomas Cavalier-Smith (1942–2021) 纪念:托马斯·卡瓦利-史密斯(1942-2021)。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-12-07 DOI: 10.1111/jeu.13013

Gaytha A. Langlois, Sonja Rueckert

引用次数: 0

Morphology, morphogenesis, and molecular characterization of Castula specialis sp. nov. (Ciliophora, Armophorea, Metopida) Castula speciis sp. 11 .的形态、形态发生和分子特征(纤毛目、甲壳目、mettopida)。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-29 DOI: 10.1111/jeu.13014

Xiaochen Feng, Daniel Méndez-Sánchez, Wenbao Zhuang, Ran Li, Ondřej Pomahač, Ivan Čepička, Johana Rotterová, Xiaozhong Hu

{"title":"Morphology, morphogenesis, and molecular characterization of Castula specialis sp. nov. (Ciliophora, Armophorea, Metopida)","authors":"Xiaochen Feng, Daniel Méndez-Sánchez, Wenbao Zhuang, Ran Li, Ondřej Pomahač, Ivan Čepička, Johana Rotterová, Xiaozhong Hu","doi":"10.1111/jeu.13014","DOIUrl":"10.1111/jeu.13014","url":null,"abstract":"The morphology, morphogenesis, and molecular phylogeny of a new metopid ciliate, Castula specialis sp. nov., comprising three strains from geographically distant (China, Mexico, Czech Republic) anoxic freshwater habitats, were studied based on microscopic observation of live and protargol-stained specimens as well as SSU rRNA gene sequence data. The new species is characterized as follows: size in vivo 105–220 × 25–70 μm, body oblong to elongated ellipsoidal and asymmetrical; preoral dome distinctly projecting beyond the body; 32–46 adoral membranelles; 31–52 somatic kineties; and 4–7 setae. This study brings the first morphogenetic investigation of a member of the genus Castula. The morphogenesis of the type population (China) of the new species proceeds as in Metopus spp. comprising drastic changes in body shape and a pleurotelokinetal stomatogenesis; however, the main difference is the origin of the opisthe's paroral membrane that derives from all perizonal rows and some adjacent dome kineties. Phylogenetically, the genus Castula is paraphyletic.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang 新疆南部和田黑鸡囊虫属的分子特征。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-17 DOI: 10.1111/jeu.13012

Xinwei Feng, Luyao Xin, Bowen Zhang, Zhichao Wang, Zhu Meng, Fuchang Yu, Meng Qi

{"title":"Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang","authors":"Xinwei Feng, Luyao Xin, Bowen Zhang, Zhichao Wang, Zhu Meng, Fuchang Yu, Meng Qi","doi":"10.1111/jeu.13012","DOIUrl":"10.1111/jeu.13012","url":null,"abstract":"To determine the infection status and assess the zoonotic potential of Blastocystis spp. in Hotan Black chickens in southern Xinjiang, China, fecal samples were collected from 617 chickens on 18 large-scale farms. The presence of Blastocystis spp. was determined using polymerase chain reaction based on the small subunit rRNA (SSU rRNA) locus. The results revealed an overall infection rate of 26.3% (162/617). Samples from Farm 1 in Luopu County showed the highest infection rate (76.3%, 29/38). The highest and lowest infection rates were detected in the <30-day (34.4%, 43/125) and > 90-day age groups (12.4%, 11/89), respectively. The infection rate decreased with increasing age. Statistical analysis showed significant differences in the infection rates of Blastocystis spp. among the different sampling sites (p < 0.05) and age groups (p < 0.05). Four Blastocystis spp. subtypes (ST6, ST7, ST10, and ST23) were identified. The infection rates of the zoonotic subtypes, ST6 and ST7, were 3.2% (20/617) and 22.2% (137/617), respectively. The presence of Blastocystis spp. and zoonotic subtypes provided evidence for the potential transmission of this pathogen between Hotan Black chickens and humans, especially in animal handlers in this area.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136397686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhabdamoeba marina is a heterotrophic relative of chlorarachnid algae 卤虫是氯虫藻类的异养亲戚。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-08 DOI: 10.1111/jeu.13010

Takashi Shiratori, Ken-ichiro Ishida

引用次数: 0

Editorial Acknowledgment 编辑致谢。

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2023-11-06 DOI: 10.1111/jeu.13006

引用次数: 0