Journal of Eukaryotic Microbiology最新文献

Engineering Anaerobic Metabolic Branch Points to Control Carbon Allocation Between Wax Ester Synthesis and Succinate Accumulation in Euglena gracilis 工程厌氧代谢分支指向控制细叶茅蜡酯合成与琥珀酸积累之间的碳分配。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-10-11 DOI: 10.1111/jeu.70134

Rikuto Oishi, Ryunosuke Katayama, Mitsuhiro Ueda, Tatsuji Sakamoto, Masami Nakazawa

{"title":"Engineering Anaerobic Metabolic Branch Points to Control Carbon Allocation Between Wax Ester Synthesis and Succinate Accumulation in Euglena gracilis","authors":"Rikuto Oishi, Ryunosuke Katayama, Mitsuhiro Ueda, Tatsuji Sakamoto, Masami Nakazawa","doi":"10.1111/jeu.70134","DOIUrl":"10.1111/jeu.70134","url":null,"abstract":"Under anaerobic conditions, Euglena gracilis degrades paramylon and synthesizes wax esters (WEs) while accumulating succinate in the culture supernatant. Succinate can either be converted to succinyl-CoA by succinyl-CoA synthetase (SCS) and enter the propionyl-CoA biosynthetic pathway or accumulate extracellularly. Propionyl-CoA serves as a primer for odd-chain WE synthesis. Prior research revealed that knockout (KO) of the SCS α-subunit (SCSα) nearly eliminated odd-chain WE synthesis while preserving overall WE accumulation. In the present study, the effects of targeted modifications at anaerobic metabolic branch points on carbon allocation between WE synthesis and succinate accumulation were examined. SCSα KO increased succinate accumulation and markedly reduced odd-chain WEs while largely maintaining total WE levels. Silencing mitochondrial NADP+-dependent malic enzyme (ME), which affects carbon allocation at the malate branch point between WE synthesis and the fumarate-to-succinate pathway, also increased succinate accumulation, although total WE accumulation decreased only slightly. Combining ME silencing with SCSα KO increased succinate accumulation to 8.9-fold that of the wild type while retaining more than 75% of the WT total WE level. These findings demonstrate that targeted modification of anaerobic metabolic branch points can redirect carbon allocation toward succinate accumulation without abolishing WE biosynthesis in E. gracilis.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial Diversity of Marine Protists Along the Northern Norway Fjords During the Sub-Arctic Summer 亚北极夏季挪威北部峡湾海洋原生生物的空间多样性

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-10-10 DOI: 10.1111/jeu.70133

Sandra Gran-Stadniczeñko, Konstantinos Anestis, Carina Bunse, Bente Edvardsen, Philipp Egbers, Rohan Henkel, Cora Hörstmann, Kerstin Klemm, Nancy Kühne, Franco Moritz, Stefan Neuhaus, Luka Šupraha, Jan Tebben, Daniela Voss, Sabrina N. Wilms, Sylke Wohlrab, Uwe John

{"title":"Spatial Diversity of Marine Protists Along the Northern Norway Fjords During the Sub-Arctic Summer","authors":"Sandra Gran-Stadniczeñko, Konstantinos Anestis, Carina Bunse, Bente Edvardsen, Philipp Egbers, Rohan Henkel, Cora Hörstmann, Kerstin Klemm, Nancy Kühne, Franco Moritz, Stefan Neuhaus, Luka Šupraha, Jan Tebben, Daniela Voss, Sabrina N. Wilms, Sylke Wohlrab, Uwe John","doi":"10.1111/jeu.70133","DOIUrl":"https://doi.org/10.1111/jeu.70133","url":null,"abstract":"Polar coastal waters are highly complex and productive ecosystems that experience increasingly higher average water temperatures, shallower and stronger stratification, and higher glacier-melting rates. Yet, the environmental complexity and high microbial diversity within fjords pose challenges to understanding the impacts of these changes and the ecological processes driving them. Here, we studied protists and prokaryote communities, their distribution, and functional ecology along the northern Norwegian coast in summer 2019 using SSU rRNA gene metabarcoding. Physicochemical data provided insights into the main drivers for differences in plankton communities. We found significant differences in alpha and beta diversity among the different fjords and between the western (Vesterålen, Balsfjorden, and Lyngenfjord) and northern areas (Porsangerfjord, Laksefjord, and Tanafjord). Temperature, salinity, and phosphate are the main drivers for community composition differences, positively correlating with the western fjords and negatively with the northern ones. Metabarcoding analyses revealed dinoflagellates, cryptophytes, and ochrophytes as the most abundant groups on the north Norwegian coast and confirmed the bloom of the ichthyotoxic haptophyte Chrysochromulina leadbeateri in Balsfjorden. Our comprehensive spatial study of communities and key contributors across different fjords set a baseline for future environmental monitoring of these unique and productive ecosystems, which are subject to rapid environmental changes.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149146127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, Molecular Phylogenetic, and Mitochondrial Genome Characterization of a Deep-Branching Marine Flagellate, Katablepharis ulleungensis sp. nov. (Cryptista) 深海分支海洋鞭毛虫Katablepharis ulleungensis sp. 11 .（隐门目）的形态、分子系统发育和线粒体基因组特征

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-10-07 DOI: 10.1111/jeu.70126

Yasinee Phanprasert, Hyeon Been Lee, Jong Im Kim, Sun Young Kim, Ophelie Marie Lamothe, Nam Seon Kang, Dong Hyuk Jeong, Jong Soo Park, Eunsoo Kim

{"title":"Morphological, Molecular Phylogenetic, and Mitochondrial Genome Characterization of a Deep-Branching Marine Flagellate, Katablepharis ulleungensis sp. nov. (Cryptista)","authors":"Yasinee Phanprasert, Hyeon Been Lee, Jong Im Kim, Sun Young Kim, Ophelie Marie Lamothe, Nam Seon Kang, Dong Hyuk Jeong, Jong Soo Park, Eunsoo Kim","doi":"10.1111/jeu.70126","DOIUrl":"https://doi.org/10.1111/jeu.70126","url":null,"abstract":"<div>\u0000 \u0000 Katablepharids (or kathablepharids) are plastid-less, biflagellate protists widely distributed in marine and freshwater environments. Here, we report a new katablepharid species, Katablepharis ulleungensis sp. nov., isolated from a black sandy beach in Ulleung-do, South Korea. The stock strain was co-cultured with a small bicosoecid flagellate, serving as its prey. Cells were oblong, displayed two rows of Type I ejectisomes, and possessed two subapically inserted flagella with the posterior flagellum slightly longer than the anterior one. Rather than exhibiting linear or spiral locomotion, cells rotated in place using both flagella. Transmission and scanning electron microscopy revealed two types of ejectisomes, each consisting of two coiled ribbons. The feeding apparatus, situated at the cell apex, comprised a cytostome, 9–10 cytopharyngeal rings, numerous putative enzymatic vesicles, and inner microtubular arrays. Phylogenetic analyses using 18S rRNA gene placed the isolate within the genus Katablepharis; the phylogenetic placement together with distinct morphological characteristics supports its recognition as a new species. Using Illumina data, the complete mitochondrial genome (35,191 bp) was assembled and annotated to contain 60 genes, including 33 protein-coding genes, 25 tRNAs, and 2 rRNAs. This study expands our understanding of katablepharid diversity and contributes to mitochondrial genome resources for marine protists.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149100040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microeukaryotic Diversity and Habitat Structuring in a Desiccating Alkaline Maar Lake (Central Mexico) 墨西哥中部干旱碱性Maar湖微真核生物多样性及生境结构

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-10-06 DOI: 10.1111/jeu.70127

Barbara Moguel, Mariano Cerca, Janet Sanchez-Sanchez, Israel Muñoz-Velasco, Marina Vega-González, Gabriela Olmedo-Álvarez

{"title":"Microeukaryotic Diversity and Habitat Structuring in a Desiccating Alkaline Maar Lake (Central Mexico)","authors":"Barbara Moguel, Mariano Cerca, Janet Sanchez-Sanchez, Israel Muñoz-Velasco, Marina Vega-González, Gabriela Olmedo-Álvarez","doi":"10.1111/jeu.70127","DOIUrl":"https://doi.org/10.1111/jeu.70127","url":null,"abstract":"Microeukaryotes are important components of microbial food webs in saline and alkaline lakes, yet their diversity and distribution across distinct microhabitats remain poorly understood. We characterized microeukaryotic communities associated with microbialites, microbial mats, hypersaline waters, and sediments in the desiccating maar lake Rincón de Parangueo (central Mexico) using 18S rRNA gene amplicon sequencing. After quality filtering and rarefaction, 417 amplicon sequence variants (ASVs) belonging to 17 eukaryotic phyla were identified. Community composition differed among microhabitats, with mats harboring the highest number of exclusive genera and water containing the fewest. No genus was shared across all four microhabitats. The greatest taxonomic overlap occurred between mats and water, suggesting ecological connectivity between benthic and planktonic environments. Ordination analyses and environmental data indicated strong habitat filtering associated primarily with salinity and conductivity. Co-occurrence network analysis revealed a highly modular community structure (Q = 0.789), with distinct habitat-associated modules and limited connectivity among them. Chlorophyta dominated assemblages, particularly in microbialites and water, whereas Bacillariophyta, Amoebozoa, Discoba, Aphelidiomycota, and Rotifera were present. Recurrent taxa such as Dunaliella and Picocystis occupied central network positions. These results demonstrate strong microhabitat-scale structuring and highlight the importance of fine-scale environmental heterogeneity in shaping diversity within alkaline maar lakes.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149099421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

All Different All Equal: High Polymorphism in Nebela (Amoebozoa: Arcellinida) Revealed by Additional Sampling and Barcoding 全异全等：附加采样和条形码分析揭示了Nebela（变形虫目：Arcellinida）的高多态性

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-10-06 DOI: 10.1111/jeu.70129

Guillermo Díez-González, David Singer, Enrique Lara

{"title":"All Different All Equal: High Polymorphism in Nebela (Amoebozoa: Arcellinida) Revealed by Additional Sampling and Barcoding","authors":"Guillermo Díez-González, David Singer, Enrique Lara","doi":"10.1111/jeu.70129","DOIUrl":"https://doi.org/10.1111/jeu.70129","url":null,"abstract":"Hyalospheniids are a conspicuous group of testate amoebae whose rich morphology has allowed their classification based on the characterization of their shells. However, recent studies showed the occurrence of convergences and phenotypic plasticity, which complicate their taxonomy. Molecular barcoding is the method of choice to resolve this diversity in an objective manner and accurately delineate species. In the genus Nebela, species diagnosis is based on small differences in shell size and shape, but sampling is scarce and restricted to a few locations, which could underestimate the real phenotypic variability. In this study, we obtained Nebela specimens from a new sampling location in Spain, which were all identified as Nebela collaris through COI barcoding and phylogenetic analysis. We showed through a morphological analysis (PCA and MRA) on 51 collected N. collaris specimens a considerable morphological variation that not only exceeds the diagnosed range for the species but also overlapped with most Nebela species, with only N. rotunda remaining clearly distinct in shape space. This illustrates the importance of intraspecific morphological variability in Arcellinida, and therefore the need for a large sampling to describe species morphology. The results stress the idea that single-cell barcoding is key in Arcellinida taxonomy.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149099418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of Ascetosporeans Associated With Feather Hydroids in New Caledonia 新喀里多尼亚与羽状水螅体相关的Ascetosporeans的多样性

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-10-06 DOI: 10.1111/jeu.70130

Akinori Yabuki, Aya Yamazaki, Takehisa Yamakita, Tetsuya Miwa, Tatsuhiro Fukuba, Karine Olu, Takashi Toyofuku

{"title":"Diversity of Ascetosporeans Associated With Feather Hydroids in New Caledonia","authors":"Akinori Yabuki, Aya Yamazaki, Takehisa Yamakita, Tetsuya Miwa, Tatsuhiro Fukuba, Karine Olu, Takashi Toyofuku","doi":"10.1111/jeu.70130","DOIUrl":"https://doi.org/10.1111/jeu.70130","url":null,"abstract":"Ascetosporea is a group of parasitic protists within Endomyxa that primarily infect marine invertebrates and can cause serious damage to fishery resources such as oysters and prawns. Although approximately 50 species have been formally described, environmental DNA (eDNA) studies suggest that their diversity remains largely underestimated, with many lineages yet to be discovered. Their ecological roles and host ranges are also poorly understood, partly because no complete life cycle has been elucidated, likely due to the difficulty of maintaining live cells in laboratory conditions. In this study, we investigated feather hydroids as potential hosts of ascetosporeans in New Caledonia, where this group has not been previously examined. Using metabarcoding with ascetosporean-specific primers, we detected 46 operational taxonomic units (OTUs) from three hydroid individuals. Of these, 31 OTUs were affiliated with environmental clades ENDO-3a and ENDO-2, whereas two and four OTUs were assigned to Paradinida and Haplosporida, respectively. Notably, seven OTUs were consistently detected across all samples, suggesting that they may have been broadly associated with the feather hydroids in this environment. These findings indicate that ascetosporean diversity and host associations in marine ecosystems are substantially underestimated.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149099419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Summer 2026 Editor-In-Chief Message 2026夏季总编留言。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-09-24 DOI: 10.1111/jeu.70131

Joel B. Dacks

引用次数: 0

Phylogenetic Placement and Morphological Description of Three Species of Heterotrophic Euglenids (Euglenozoa) From Arizona: Scytomonas flumensis n. sp., Ploeotia protuberantia n. sp., and Hokulea pusilla comb. nov 美国亚利桑那州三种异养真藻类的系统发育定位和形态描述：Scytomonas fluumensis n. sp.、Ploeotia protuberantia n. sp.和Hokulea pusilla comb.。11月。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-09-22 DOI: 10.1111/jeu.70128

Maia V. Palka, Alfredo Rodriguez-Ruiz, Gordon Lax, Yesenia Garza, Brian S. Leander, Jeremy G. Wideman

{"title":"Phylogenetic Placement and Morphological Description of Three Species of Heterotrophic Euglenids (Euglenozoa) From Arizona: Scytomonas flumensis n. sp., Ploeotia protuberantia n. sp., and Hokulea pusilla comb. nov","authors":"Maia V. Palka, Alfredo Rodriguez-Ruiz, Gordon Lax, Yesenia Garza, Brian S. Leander, Jeremy G. Wideman","doi":"10.1111/jeu.70128","DOIUrl":"10.1111/jeu.70128","url":null,"abstract":"Euglenids are microbial flagellates best known for their highly flexible photosynthetic species (e.g., Euglena gracilis), though many heterotrophic groups remain poorly characterized at both molecular and ultrastructural levels. In particular, rigid heterotrophs such as ploeotids and petalomonads are highly diverse, yet most described species were documented only with light microscopy, largely because they are difficult to cultivate. Here, we establish cultures for three new isolates of ploeotids and petalomonads (Hokulea pusilla comb. nov., Ploeotia protuberantia sp. nov., Scytomonas flumensis sp. nov.) and examine them using light microscopy, scanning electron microscopy, and 18S rDNA phylogenetic analyses. These data support the recognition of two new species, Ploeotia protuberantia sp. nov. and Scytomonas flumensis sp. nov., and justify the transfer of Ploeotia pusilla to Hokulea pusilla comb. nov. based on its morphology and its affiliation with Hokulea waialensis in 18S rDNA phylogenies.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of an Undocumented Symbiosis Between Arcellinida and a Flagellate Using Single-Cell Transcriptomics 利用单细胞转录组学发现一种未记载的Arcellinida和鞭毛虫共生关系。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-09-22 DOI: 10.1111/jeu.70119

Godwin N. Ani, Taylor Sehein, Giulia M. Ribeiro, Laura A. Katz

{"title":"Discovery of an Undocumented Symbiosis Between Arcellinida and a Flagellate Using Single-Cell Transcriptomics","authors":"Godwin N. Ani, Taylor Sehein, Giulia M. Ribeiro, Laura A. Katz","doi":"10.1111/jeu.70119","DOIUrl":"10.1111/jeu.70119","url":null,"abstract":"<div>\u0000 \u0000 Symbiotic interactions involving single-celled eukaryotes have been studied for over a century. This is in part because these microbes occupy nearly all environments and have adapted to survive through interactions with other species. Among them, Arcellinida—amoebae enclosed in a test (i.e., shell)—have been studied extensively since the 1800s. The preservability of their tests, and sensitivity to environmental conditions, has made them important in studies of modern and paleoenvironments. However, despite centuries of observation, we have seen no record of protists living within Arcellinida tests. Here, we investigate a symbiosis involving an unknown flagellate found swimming inside the test of multiple Arcellinida species from a site in Acadia National Park (Maine, USA). We analyzed 125 single-cell Arcellinida transcriptomes from five genera, of which 10 individuals had a flagellate living inside the test at the time of sequencing. By combining microscopy, molecular, and bioinformatic methods, we identify this novel flagellate as belonging to the Prokinetoplastid clade. We also make inferences on the nature of the relationship through analysis of the host gene expression. Overall, our results provide a framework for studying microbial symbiosis in uncultivable groups and adds to our knowledge of interspecific interactions at the microbial level.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

tArp Is an Actin-Related Protein Required for Ciliary Function That Is Conserved in Ciliates and Related Eukaryotic Groups tArp是纤毛功能所需的肌动蛋白相关蛋白，在纤毛及相关真核生物类群中保守存在

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-09-18 DOI: 10.1111/jeu.70123

Miku Kagawa, Minori Hagita, Takuro Nakayama, Kogiku Shiba, Takako Kato-Minoura, Shinya Matsuda, Kazuo Inaba, Yuji Inagaki, Kentaro Nakano

{"title":"tArp Is an Actin-Related Protein Required for Ciliary Function That Is Conserved in Ciliates and Related Eukaryotic Groups","authors":"Miku Kagawa, Minori Hagita, Takuro Nakayama, Kogiku Shiba, Takako Kato-Minoura, Shinya Matsuda, Kazuo Inaba, Yuji Inagaki, Kentaro Nakano","doi":"10.1111/jeu.70123","DOIUrl":"https://doi.org/10.1111/jeu.70123","url":null,"abstract":"Cells exhibit a wide range of behaviors due to their diverse molecular systems, which have been established through the molecular evolution of individual species. Protein families generated through gene duplications may be involved in this process. The actin cytoskeleton is no exception. The majority of eukaryotes express not only actin, but also several types of actin-related proteins (Arps). The ciliate Tetrahymena thermophila possesses a species-specific Arp, named tArp, which localizes to cilia. However, the evolutionary origin of the gene and its detailed cellular functions remain unclear. In the present study, we performed a comprehensive molecular phylogenetic analysis of actin and Arps across eukaryotes, with a focus on ciliates and their close relatives, and demonstrated that tArp is widely conserved in SAR, one of the largest eukaryotic supergroups consisting of Stramenopiles, Alveolata, and Rhizaria. Furthermore, the gene disruption of tARP reduced swimming velocity due to defects in ciliary beat frequency and waveforms. Therefore, we propose that tArp is a member of the novel Arp family that specifically contributes to efficient ciliary motility.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0