Journal of Eukaryotic Microbiology最新文献

Fine structural features of the free-living stages of Amyloodinium ocellatum (Dinoflagellata, Thoracosphaeraceae): A marine fish ectoparasite. Amyloodinium ocellatum（甲壳纲，Thoracosphaeraceae）自由生活阶段的精细结构特征：一种海洋鱼类外寄生虫。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-11-18 DOI: 10.1111/jeu.13067

Zhicheng Li, Jingyu Zhuang, Jizhen Cao, Qing Han, Zhi Luo, Baotun Wang, Hebing Wang, Chuanfu Dong, Anxing Li

{"title":"Fine structural features of the free-living stages of Amyloodinium ocellatum (Dinoflagellata, Thoracosphaeraceae): A marine fish ectoparasite.","authors":"Zhicheng Li, Jingyu Zhuang, Jizhen Cao, Qing Han, Zhi Luo, Baotun Wang, Hebing Wang, Chuanfu Dong, Anxing Li","doi":"10.1111/jeu.13067","DOIUrl":"https://doi.org/10.1111/jeu.13067","url":null,"abstract":"Amyloodinium ocellatum is a protozoan parasite that causes amyloodiniosis in marine and brackish water fish, threatening global aquaculture. The present study investigates the morphology and ultrastructure of the free-living stages of A. ocellatum (tomont and dinospore) using light microscopy (LM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Dinospores measured 13.03-19.66 μm in length, 12.32-18.71 μm in width, and were laterally flattened. Dinospores had a transverse flagellum for propulsion and a longitudinal flagellum for direction control. The cyst wall had three distinct layers and included cellulose. The outer wall was coated with numerous bacteria. The orange-red speckled eyespot was observed all tomont developmental stages and in the dinospore of A. ocellatum. Tomonts proliferation required successive nuclear division, the formation of new cyst walls, and cytoplasmic segregation. The cytoplasm comprises mainly the matrix, organelles, and inclusions. The matrix was grainy and evenly distributed. In addition to organelles, including mitochondria with tubular cristae, Golgi apparatus, and endoplasmic reticulum, the cytoplasm had starch grains and lipid droplets as inclusions. The A. ocellatum cells lacked chloroplasts. This study provides the first ultrastructural view of the cytoplasmic structure of the free-living stages of A. ocellatum.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":" ","pages":"e13067"},"PeriodicalIF":2.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of protease inhibitors on the intraerythrocytic development of Babesia microti and Babesia duncani, the causative agents of human babesiosis. 蛋白酶抑制剂对人类巴贝西亚原虫小巴贝西亚和邓卡尼巴贝西亚红细胞内发育的影响。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-11-18 DOI: 10.1111/jeu.13064

Temitope Aderanti, Jordan M Marshall, Jose Thekkiniath

{"title":"Effect of protease inhibitors on the intraerythrocytic development of Babesia microti and Babesia duncani, the causative agents of human babesiosis.","authors":"Temitope Aderanti, Jordan M Marshall, Jose Thekkiniath","doi":"10.1111/jeu.13064","DOIUrl":"https://doi.org/10.1111/jeu.13064","url":null,"abstract":"Human babesiosis is a malaria-like, tick-borne infectious disease with a global distribution. Babesiosis is caused by intraerythrocytic, apicomplexan parasites of the genus Babesia. In the United States, human babesiosis is caused by Babesia microti and Babesia duncani. Current treatment for babesiosis includes either the combination of atovaquone and azithromycin or the combination of clindamycin and quinine. However, the side effects of these agents and the resistance posed by these parasites call for alternative approaches for treating human babesiosis. Proteases play several roles in the context of parasitic lifestyle and regulate basic biological processes including cell death, cell progression, and cell migration. Using the SYBR Green-1 assay, we screened a protease inhibitor library that consisted of 160 compounds against B. duncani in vitro and identified 13 preliminary hits. Dose response assays of hit compounds against B. duncani and B. microti under in vitro conditions identified five effective inhibitors against parasite growth. Of these compounds, we chose ixazomib, a proteasome inhibitor as a potential drug for animal studies based on its lower IC50 and a higher therapeutic index in comparison with other compounds. Our results suggest that Babesia proteasome may be an important drug target and that developing this class of drugs may be important to combat human babesiosis.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":" ","pages":"e13064"},"PeriodicalIF":2.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broad-range necrophytophagy in the flagellate Orciraptor agilis (Viridiraptoridae, Cercozoa) and the underappreciated role of scavenging among protists. 鞭毛虫Orciraptor agilis（Viridiraptoridae，Cercozoa）的大范围食腐作用以及原生动物中未得到充分重视的清道夫作用。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-11-03 DOI: 10.1111/jeu.13065

Jannika Moye, Sebastian Hess

引用次数: 0

The identity of Centrodinium elongatum, type species of the dinoflagellate genus Centrodinium (Dinophyceae), and a review on the synonymy of allied species. Centrodinium elongatum（甲藻属 Centrodinium 的模式种）的身份及相关物种的同义综述。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-10-10 DOI: 10.1111/jeu.13062

Fernando Gómez, Tania Corina Navarrete-Carlos, Yahir Enrique López-Osorio, Huan Zhang, Eugenio Raymond, Rafael Salas, Rosalba Alonso-Rodríguez, Senjie Lin

{"title":"The identity of Centrodinium elongatum, type species of the dinoflagellate genus Centrodinium (Dinophyceae), and a review on the synonymy of allied species.","authors":"Fernando Gómez, Tania Corina Navarrete-Carlos, Yahir Enrique López-Osorio, Huan Zhang, Eugenio Raymond, Rafael Salas, Rosalba Alonso-Rodríguez, Senjie Lin","doi":"10.1111/jeu.13062","DOIUrl":"https://doi.org/10.1111/jeu.13062","url":null,"abstract":"The planktonic dinoflagellate genus Centrodinium has been understudied, with the type species C. elongatum remaining undocumented since the original description. Here, we report C. elongatum isolated from Mazatlán, Mexican Pacific. In the chains, the posterior daughter cell with an incomplete apical horn shows the morphology of C. elongatum, while the anterior daughter cell with complete epitheca corresponds to C. pulchrum. For the first time, a species of Centrodinium sensu stricto (highly laterally flattened species with horns) was cultured. An unarmored life stage, known as Murrayella ovalis, derived from the spheroplast after ecdysis. In the rDNA molecular phylogenies, C. elongatum (=C. pulchrum) nested as basal to morphologically similar species (C. eminens and C. intermedium) and as a sister group of a former Murrayella species, C. punctatum. C. elongatum differs from C. eminens and C. intermedium in the chain formation, second apical (2') plate not being divided, horns with coarse poroid ornamentation, and missing prominent distal spinules. The taxonomy of Centrodinium sensu stricto is revised, with a discussion in the identities of C. complanatum, C. eminens, and C. maximum. The name C. deflexum is restored as a senior synonym of C. intermedium and C. ovale.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":" ","pages":"e13062"},"PeriodicalIF":2.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refurbishing the marine parasitoid order Pirsoniales with newly (re)described marine and freshwater free-living predators. 利用新近（重新）描述的海洋和淡水自由生活捕食者，重建海洋寄生虫目（Pirsoniales）。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-10-01 DOI: 10.1111/jeu.13061

Kristina I Prokina, Naoji Yubuki, Denis V Tikhonenkov, Maria Christina Ciobanu, Purificación López-García, David Moreira

{"title":"Refurbishing the marine parasitoid order Pirsoniales with newly (re)described marine and freshwater free-living predators.","authors":"Kristina I Prokina, Naoji Yubuki, Denis V Tikhonenkov, Maria Christina Ciobanu, Purificación López-García, David Moreira","doi":"10.1111/jeu.13061","DOIUrl":"https://doi.org/10.1111/jeu.13061","url":null,"abstract":"Pirsoniales is a stramenopile order composed of marine parasitoids of diatoms with unique life cycle. Until recently, a single genus, Pirsonia, uniting six species, was known. The recent identification of new free-living eukaryotrophic Pirsoniales Pirsonia chemainus, Feodosia pseudopoda, and Koktebelia satura changed our understanding of this group as exclusively parasitic. However, their cell ultrastructure and feeding preferences were not fully studied due to the death of the cultures. In this study, we re-isolated some of these Pirsoniales and established six new strains exhibiting predatory behavior, including a first freshwater representative. This allowed us to describe five new genera and species, as well as to emend the diagnosis of the order Pirsoniales. The 18S rRNA gene phylogenetic analysis revealed the position of new strains within Pirsoniales and their relationships with parasitoid relatives and environmental sequence lineages. Feeding experiments on novel Pirsoniales strains using diverse algal prey showed that they were not able to form trophosomes and auxosomes. The ability of cell aggregation in Pirsoniales was observed for the first time. One of the studied strains contained intracellular gammaproteobacteria distantly related to Coxiella. Ultrastructural analyses revealed a more complex cytoskeleton structure in Pirsoniales than previously thought and supported the monophyly of Bigyromonadea and Pseudofungi.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":" ","pages":"e13061"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomics of Diphyllatea (CRuMs) from South Pacific crater lakes confirm new cryptic clades. 南太平洋陨石坑湖中的 Diphyllatea（CRuMs）转录组学证实了新的隐秘支系。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-09-28 DOI: 10.1111/jeu.13060

Luis Javier Galindo, Varsha Mathur, Hadleigh Frost, Guifré Torruella, Thomas A Richards, Nicholas A T Irwin

引用次数: 0

Ultrastructural and molecular characterization of Glugea sp. (microsporidia), a parasite of the Red Sea fish Carangoides bajad (Carangidae) 红海鱼类 Carangoides bajad（鲤科）的寄生虫 Glugea sp.（微孢子虫）的超微结构和分子特征描述

IF 2.2 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-09-10 DOI: 10.1111/jeu.13058

Abdel‐Azeem S. Abdel‐Baki, Shawky M. Aboelhadid, Heba Abdel‐Tawab, Sónia Rocha, Manal Ahmed, Saleh Al‐Quraishy, Lamjed Mansour

{"title":"Ultrastructural and molecular characterization of Glugea sp. (microsporidia), a parasite of the Red Sea fish Carangoides bajad (Carangidae)","authors":"Abdel‐Azeem S. Abdel‐Baki, Shawky M. Aboelhadid, Heba Abdel‐Tawab, Sónia Rocha, Manal Ahmed, Saleh Al‐Quraishy, Lamjed Mansour","doi":"10.1111/jeu.13058","DOIUrl":"https://doi.org/10.1111/jeu.13058","url":null,"abstract":"Glugea sp. found infecting the liver of the teleost fish Carangoides bajad from the Red Sea, Egypt, is described based on light microscopy and ultrastructural characteristics combined with phylogenetic analyses. This microsporidium forms whitish xenomas up to ~4 mm in size. Xenomas display numerous parasitophorous vacuoles totally filled by mature spores, no other life cycle stages were observed. Mature spores ellipsoidal and measuring 6.3 × 4.0 μm in size. The polaroplast appears composed of two distinct regions: an electron‐dense vesicular region and a densely packed lamellar region. The polar tubule forms approximately 24–27 coils arranged in three layers encircling the posterior vacuole. The small subunit (SSU) rRNA gene and its ITS region were sequenced and showed the highest similarity of 99.4% to other Glugea spp. Bayesian inference and maximum likelihood analyses place the novel isolate within the Glugea clade, more specifically within a subclade that predominantly grouped species described from fish inhabiting the Arabian Gulf or Red Sea. The results validate the parasite's classification in the Glugea genus. Nevertheless, until more detailed ultrastructural and molecular data are obtained, the identification of the current Glugea species is hampered by the absence of some developmental stages and the high degree of genetic similarity.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"387 1","pages":"e13058"},"PeriodicalIF":2.2,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142180383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microsporidia secretory effectors and their roles in pathogenesis 小孢子虫分泌效应器及其在致病过程中的作用。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-09-04 DOI: 10.1111/jeu.13046

Maoshuang Ran, Wenxin Yang, Muhammad Usman Faryad Khan, Tian Li, Guoqing Pan

{"title":"Microsporidia secretory effectors and their roles in pathogenesis","authors":"Maoshuang Ran, Wenxin Yang, Muhammad Usman Faryad Khan, Tian Li, Guoqing Pan","doi":"10.1111/jeu.13046","DOIUrl":"10.1111/jeu.13046","url":null,"abstract":"Microsporidia, a group of unicellular eukaryotic parasites, rely intensely on secretory effectors for successful invasion and proliferation within host cells. This review focuses on the identification, characterization, and functional roles of effectors, including secretory proteins and microRNAs. The adhesion proteins like the Ricin-B-lectin facilitate initial invasion, which binds to the host cell surface. Once inside, microsporidia deploy a range of effectors to modulate host immune responses, such as serpin proteins, and redirect host cell metabolism to meet the parasite's nutritional needs through hexokinase. Some effectors such as microRNAs, alter the host gene expression to create a more favorable intracellular parasitic environment. In conclusion, the secretory effectors of microsporidia play a pivotal role spanning from host cell invasion to intracellular establishment. In the future, more effectors secreted by microsporidia will be studied, which will not only help to elucidate the molecular mechanism of pathogenic manipulation of the host but also help to provide the potential targets for anti-parasitic treatments.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"71 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Skoliomonas gen. nov., a haloalkaliphilic anaerobe related to barthelonids (Metamonada). Skoliomonas gen.nov.的特征，这是一种与巴特龙类（Metamonada）有关的卤代嗜碱性厌氧菌。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-09-03 DOI: 10.1111/jeu.13048

Yana Eglit, Shelby K Williams, Andrew J Roger, Alastair G B Simpson

{"title":"Characterization of Skoliomonas gen. nov., a haloalkaliphilic anaerobe related to barthelonids (Metamonada).","authors":"Yana Eglit, Shelby K Williams, Andrew J Roger, Alastair G B Simpson","doi":"10.1111/jeu.13048","DOIUrl":"https://doi.org/10.1111/jeu.13048","url":null,"abstract":"Metamonads are a large and exclusively anaerobic group of protists. Additionally, they are one of the three clades proposed to ancestrally possess an \"excavate\" cell morphology, with a conspicuous ventral groove accompanied by a posterior flagellum with a vane. Here, we cultivate and characterize four anaerobic bacterivorous flagellates from hypersaline and alkaline soda lake environments, which represent a novel clade. Small subunit ribosomal RNA (SSU rRNA) gene phylogenies support recent phylogenomic analyses in placing them as the sister of barthelonids, a group that is itself sister to or deeply branching within Fornicata (Metamonada). The new isolates have a distinctive morphology: the hunchbacked cell body is traversed by a narrow ventral groove ending in a large opening to a conspicuous recurrent cytopharynx. The right margin of the groove is defined by a thin \"lip.\" The posterior flagellum bears a wide ventral-facing vane. The narrow ventral groove and elongate cytopharynx are shared with barthelonids. We describe one isolate as Skoliomonas litria, gen. et sp. nov. Further investigation of their mitochondrial-related organelles (MROs) and detailed ultrastructural studies would be important to understanding the adaptation to anaerobic conditions in Metamonads-especially fornicates-as well as the evolution of the \"excavate\" cell architecture.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":" ","pages":"e13048"},"PeriodicalIF":2.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HCN channels are essential for the escape response of Paramecium. HCN通道对鹦鹉螺的逃逸反应至关重要。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-08-28 DOI: 10.1111/jeu.13057

Daisuke Kandabashi, Mutsumi Kawano, Shinobu Izutani, Hiyori Harada, Takashi Tominaga, Manabu Hori

{"title":"HCN channels are essential for the escape response of Paramecium.","authors":"Daisuke Kandabashi, Mutsumi Kawano, Shinobu Izutani, Hiyori Harada, Takashi Tominaga, Manabu Hori","doi":"10.1111/jeu.13057","DOIUrl":"https://doi.org/10.1111/jeu.13057","url":null,"abstract":"When mechanical stimulation was applied to free swimming Paramecium, forward swimming velocity transiently increased due to activation of the posterior mechanosensory channels. The behavior response, known as \"escape response,\" requires membrane hyperpolarization and the activation of K-channel type adenylate cyclases. Our hypothesis is that this escape response also involves activation of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. HCN channels are activated by hyperpolarization and are modulated by cyclic nucleotides such as cAMP and cGMP. They play a critical role in many excitable cells in higher animals. If HCN channels act in Paramecium, this should help to enhance and prolong hyperpolarization, thereby increasing the swimming speed of Paramecium. This study used RNAi to examine the role of the HCN channel 1 in the escape responses by generating hcn1-gene knockdown cells (hcn1-KD). These cells showed reduced mechanically-stimulated escape responses and a lack of cGMP-dependent increases in swimming speed. Electrophysiological experiments demonstrated reduced hyperpolarization upon injection of large negative currents in hcn1-KD cells. This is consistent with a decrease in HCN1 channel activity and changes in the escape response. These findings suggest that HCN1 channels are K+ channels that regulate the escape response of Paramecium by amplifying the hyperpolarizations elicited by posterior mechanical stimulation.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":" ","pages":"e13057"},"PeriodicalIF":2.1,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142093480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0