Journal of Eukaryotic Microbiology最新文献

Algal Symbiont Diversity and Host Fitness Variation in Amoebozoan Photosymbiosis

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-04-25 DOI: 10.1111/jeu.70008

Daisuke Yamagishi, Ryo Onuma, Sachihiro Matsunaga, Shin-ya Miyagishima, Shinichiro Maruyama

{"title":"Algal Symbiont Diversity and Host Fitness Variation in Amoebozoan Photosymbiosis","authors":"Daisuke Yamagishi, Ryo Onuma, Sachihiro Matsunaga, Shin-ya Miyagishima, Shinichiro Maruyama","doi":"10.1111/jeu.70008","DOIUrl":"https://doi.org/10.1111/jeu.70008","url":null,"abstract":"Photosymbioses, the symbiotic relationships between microalgae and non-photosynthetic eukaryotes, are sporadically found in many eukaryotic lineages. Only a few taxa, such as cnidarians and ciliates hosting algal endosymbionts, have been actively studied, which has hindered understanding the universal mechanisms of photosymbiosis establishment. In Amoebozoa, few species are reported as photosymbiotic, and how the photosymbioses are established is still unclear. To investigate the extent to which one of the photosymbiotic amoebae, Mayorella viridis, depends on their symbionts, the amoebae were treated with reagents known to induce the collapsing of photosymbioses in other species. We succeeded in removing algal symbionts from the hosts with 2-amino-3-chloro-1,4-naphthoquinone. While the apo-symbiotic amoebae grew to the same extent as the symbiotic state when they fed on prey, their survival rates were lower than those of the symbiotic ones during starvation, suggesting that the impact of the photosymbiosis on fitness is condition-dependent. Furthermore, we showed that the photosymbiotic state was reversible by feeding two strains of the green alga Chlorella to the apo-symbiotic amoebae. The efficiencies of ingesting algal cells significantly differed between algal strains. These results suggest that the photosymbiotic relationship in the amoeba is facultative and that different algal strains have discrete symbiotic abilities to the amoeba.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Foraging Mechanisms in Atypical Excavate Flagellates

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-04-17 DOI: 10.1111/jeu.70010

Sei Suzuki-Tellier, Alastair G. B. Simpson, Thomas Kiørboe

{"title":"Novel Foraging Mechanisms in Atypical Excavate Flagellates","authors":"Sei Suzuki-Tellier, Alastair G. B. Simpson, Thomas Kiørboe","doi":"10.1111/jeu.70010","DOIUrl":"https://doi.org/10.1111/jeu.70010","url":null,"abstract":"Most excavates, a paraphyletic assemblage of flagellates, typically present an active vaned flagellum that drives a feeding current through a ventral groove for predation. However, some have “atypical” morphologies. Here, we describe the foraging mechanisms in heteroloboseid flagellates (Discoba) that have a groove but lack the seemingly crucial vane. The percolomonads barbeliid AE-1 and Percolomonas doradorae form a functional vane with four adjacent flagella undulating with lateral asymmetry, creating an erratic flow that rapidly “sucks” water into the groove and expels it on the other side. This flow attenuates rapidly away from the cell, consistent with the flagellar pump acting as an instantaneous point sink. Conversely, Pharyngomonas kirbyi generates a continuous flow through the groove with two asynchronously moving posterior flagella. Despite the unexplained fluid dynamics, this flow has a further reach, consistent with describing the flagellar pump as a point force (stokeslet). While the volumetric flow rate through the groove—a measure of the maximum potential clearance rate—of the two percolomonads is similar to clearance rates estimated for other phagotrophic flagellates, it is an order of magnitude lower for Ph. kirbyi, which may afford lower rates due to high prey concentration in its hypersaline environment.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kapelodiniopsis flava n. g., n. sp. (Dinophyceae), a new katodinioid with haptophyte-derived plastids of multiple origins: Implications for the plastid integration process Kapelodiniopsis flava n. g., n. sp. (Dinophyceae), 一种新的裸子植物，其质体来源于多个源头：对质体整合过程的影响

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-04-11 DOI: 10.1111/jeu.13082

Kazuya Takahashi, Wai Mun Lum, Elisabeth Hehenberger, Mitsunori Iwataki

{"title":"Kapelodiniopsis flava n. g., n. sp. (Dinophyceae), a new katodinioid with haptophyte-derived plastids of multiple origins: Implications for the plastid integration process","authors":"Kazuya Takahashi, Wai Mun Lum, Elisabeth Hehenberger, Mitsunori Iwataki","doi":"10.1111/jeu.13082","DOIUrl":"https://doi.org/10.1111/jeu.13082","url":null,"abstract":"An autotrophic unarmored dinoflagellate species with haptophyte-derived plastids, Kapelodiniopsis flava n. g., n. sp., was described as a sister taxon of Kapelodinium vestifici, which was formerly well characterized by its low-positioned cingulum and heterotrophic nature. The isolates from several Japanese coastal locations were observed using light microscopy, scanning and transmission electron microscopy, and their phylogeny was inferred from nuclear-encoded rRNA genes and multiple plastid-encoded genes. To date of this publication, a representative culture of Ks. flava has grown autotrophically for 98 months in the absence of prey or organic matter. This dinoflagellate lacked nonplastid haptophyte cell components (e.g. nucleus or mitochondria). In the host dinoflagellate phylogeny, Ks. flava was distantly related to the other two dinoflagellate lineages known to be associated with haptophyte-derived plastids, thus representing the third of such lineage. Plastid origins differed among Ks. flava strains (>99.8% 18S rRNA gene identity), with plastids being derived from at least three haptophytes and an especially strong genetic similarity to two distantly related extant haptophytes (>99.9% 16S rRNA gene identity). This indicates that Ks. flava recently integrated plastids from multiple haptophyte lineages to an extent that allows the host to replicate the plastids without other haptophyte components.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is the Composition of Communities in Bromeliad Water and Adjacent Soil Similar?

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-04-08 DOI: 10.1111/jeu.70009

Luciano Huber, Eduardo Malfatti, Carla S. R. Huber, Joe D. Taylor, Laura R. P. Utz

{"title":"Is the Composition of Communities in Bromeliad Water and Adjacent Soil Similar?","authors":"Luciano Huber, Eduardo Malfatti, Carla S. R. Huber, Joe D. Taylor, Laura R. P. Utz","doi":"10.1111/jeu.70009","DOIUrl":"https://doi.org/10.1111/jeu.70009","url":null,"abstract":"<div>\u0000 \u0000 Soil and bromeliads are important habitats contributing to the biodiversity of the Atlantic Forest in Brazil. However, knowledge of unicellular eukaryotes and bacteria in these environments remains limited. This study compared the diversity and community structure of unicellular eukaryotes, fungi, metazoan, and bacteria in bromeliad water tanks (BWT) and adjacent soil using 16S and 18S rRNA gene metabarcoding. Communities differed significantly between habitats but shared some taxa, suggesting habitat connectivity. Ciliates dominated unicellular eukaryotes in BWT, while Cercozoa prevailed in soil. Bacterial communities were dominated by Pseudomonadota, while fungal composition was more uniform, with Ascomycota as the dominant phylum across samples. Metazoan communities varied among abundant phyla, and their presence in BWT suggests the use of this water resource within the forest. This study provides essential baseline data on eukaryotic and bacterial diversity in this unique ecosystem, highlighting how distinct habitats within the Atlantic Forest support different communities.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Record of Aplanochytrium on the Chilean Coast and its Biotechnological Potential: Expanding the Geographic Distribution of Labyrinthulomycetes

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-16 DOI: 10.1111/jeu.70007

Pamela Jara-Zapata, Patricia Arancibia-Ávila, Moisés A. Valladares, Cristian Torres-Díaz, Edgar Pastene, Nicolás Reyes-Quinteros

{"title":"First Record of Aplanochytrium on the Chilean Coast and its Biotechnological Potential: Expanding the Geographic Distribution of Labyrinthulomycetes","authors":"Pamela Jara-Zapata, Patricia Arancibia-Ávila, Moisés A. Valladares, Cristian Torres-Díaz, Edgar Pastene, Nicolás Reyes-Quinteros","doi":"10.1111/jeu.70007","DOIUrl":"https://doi.org/10.1111/jeu.70007","url":null,"abstract":"<div>\u0000 \u0000 This study reports the first record of the genus Aplanochytrium on the coast of Chile, belonging to the class Labyrinthulomycetes in the kingdom Stramenopila. These marine protists play an essential role in carbon and nitrogen cycles as decomposers in the aquatic ecosystem. Although ecologically significant, their global diversity and distribution must be better understood. Samples were collected from Corral Bay, Valdivia, Chile, and later cultivated and identified through classical taxonomy and molecular analysis. Phylogenetic analysis based on the 18S rRNA sequence showed that Chilean samples were recovered in the Aplanochytrium s.s. lineage. Besides Aplanochytrium kerguelense Bahnweg & Sparrow, 1972, found in Kerguelen, an antarctic island from the Indian Ocean, this report extends the geographic distribution of the genus from Asia and the Northern Hemisphere to South America. This finding enhances our understanding of the diversity and distribution of Labyrinthulomycetes. Additionally, the fatty acid profile of Aplanochytrium revealed a high proportion of docosahexaenoic acid (DHA), accounting for 50% of the total fatty acids, along with the presence of palmitic acid. Astaxanthin, a valuable pigment for biotechnological applications, was also quantified. These results highlight the importance of Labyrinthulomycetes in marine ecosystems and their potential in biotechnology.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and molecular phylogeny of two soil species of Holostichides (Ciliophora, Hypotrichia) collected from eastern China, including a new species and a new subspecies

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-13 DOI: 10.1111/jeu.70001

Xuetong Zhao, Yaohan Jiang, Jie Huang, Xiaotian Luo, Yang Liu, Xiangrui Chen

{"title":"Morphology and molecular phylogeny of two soil species of Holostichides (Ciliophora, Hypotrichia) collected from eastern China, including a new species and a new subspecies","authors":"Xuetong Zhao, Yaohan Jiang, Jie Huang, Xiaotian Luo, Yang Liu, Xiangrui Chen","doi":"10.1111/jeu.70001","DOIUrl":"https://doi.org/10.1111/jeu.70001","url":null,"abstract":"Newly discovered hypotrich ciliates with evolutionary innovations are challenging to the established systematics and greater interest. Based on living morphology, infraciliature, and SSU rDNA, two bakuellid taxa—including a new species and a new subspecies—collected from subtropical soil environments in eastern China were investigated in the present study. Holostichides (Extraholostichides) eastensis ningboensis n. subsp. is characterized as follows: size in vivo 150–230 × 45–60 μm; cortical granules green and spherical; 3–5 small cirri arranged longitudinally posteriorly to middle frontal cirrus; row of 19–24 frontoterminal cirri exceeding half of the body length; 4–6 caudal cirri. Holostichides (Extraholostichides) muscus n. sp. is defined as follows: size in vivo 140–175 × 40–65 μm; cortical granules green and spherical; one or two small cirri posteriorly to the middle frontal cirrus; row of 3–6 frontoterminal cirri right of the midventral complex; three or four caudal cirri. Phylogenetic analyses based on SSU rDNA sequences revealed the systematic positions of these two new taxa and supported their validity as distinct subspecies and species, respectively.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validating the Genus Pocheina (Acrasidae, Heterolobosea, Discoba) Leads to the Recognition of Three Major Lineages Within Acrasidae

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-10 DOI: 10.1111/jeu.70004

Alexander K. Tice, Kevin Regis, Timothy E. Shutt, Frederick W. Spiegel, Matthew W. Brown, Jeffrey D. Silberman

{"title":"Validating the Genus Pocheina (Acrasidae, Heterolobosea, Discoba) Leads to the Recognition of Three Major Lineages Within Acrasidae","authors":"Alexander K. Tice, Kevin Regis, Timothy E. Shutt, Frederick W. Spiegel, Matthew W. Brown, Jeffrey D. Silberman","doi":"10.1111/jeu.70004","DOIUrl":"https://doi.org/10.1111/jeu.70004","url":null,"abstract":"<div>\u0000 \u0000 Pocheina and Acrasis are two genera of heterolobosean sorocarpic amoebae within Acrasidae that have historically been considered close relatives. The two genera were differentiated based on their differing fruiting body morphologies. The validity of this taxonomic distinction was challenged when a SSU rRNA phylogenetic study placed an isolate morphologically identified as “Pocheina” rosea within a clade of Acrasis rosea isolates. The authors speculated that pocheinoid fruiting body morphology might be the result of aberrant Ac. rosea fruiting body development, which, if true, would nullify this taxonomic distinction between genera. To clarify Acrasidae systematics, we analyzed SSU rRNA and ITS region sequences from multiple isolates of Pocheina, Acrasis, and Allovahlkampfia generated by Polymerase Chain Reaction (PCR) and transcriptomics. We demonstrate that the initial SSU sequence attributed to “P. rosea” originated from an Ac. rosea DNA contamination in its amplification reaction. Our analyses, based on morphology, SSU and 5.8S rRNA gene phylogenies, as well as comparative analyses of ITS1 and ITS2 sequences, resolve Acrasidae into three major lineages: Allovahlkampfia and the strongly supported clades comprising Pocheina and Acrasis. We confirm that the latter two genera can be identified by their fruiting body morphologies.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation of Ancyromonas melba, and Reclassification as the Type Species of Divimonas gen. nov., a Phylogenetically Important Ancyromonad Lineage

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-10 DOI: 10.1111/jeu.70005

Kate M. Barkhouse, Yana Eglit, Elizabeth J. Weston, Adriana B. A. Jenkins, Alastair G. B. Simpson

{"title":"Cultivation of Ancyromonas melba, and Reclassification as the Type Species of Divimonas gen. nov., a Phylogenetically Important Ancyromonad Lineage","authors":"Kate M. Barkhouse, Yana Eglit, Elizabeth J. Weston, Adriana B. A. Jenkins, Alastair G. B. Simpson","doi":"10.1111/jeu.70005","DOIUrl":"https://doi.org/10.1111/jeu.70005","url":null,"abstract":"Ancyromonadida is a taxon of small heterotrophic flagellates occupying an unresolved but deep-branching position in the eukaryotic tree of life, thus suspected to be important to studies of early eukaryotic evolutionary relationships and the characteristics of the last eukaryotic common ancestor. Sampling and cultivation of the full diversity of ancyromonad species are therefore areas of considerable interest. Ancyromonas melba is a species originally described from hypersaline material for which no monoprotistan culture or molecular data have been available, but whose distinct morphology suggests it may represent a new major lineage within Ancyromonadida. We report the first cultivation of this morphospecies in hypersaline media, with characterization including scanning electron microscopy and small subunit rRNA gene sequencing. Distinguishing morphological features include the predominantly ventral placement of the ventral groove, the approximately equal thickness of the anterior and posterior flagella, and the relatively long anterior flagellum. Phylogenetic analysis shows that the isolate does not branch within Ancyromonas or any other currently described genus of Ancyromonadida, but represents a novel genus-level lineage, the position of which within ancyromonads could not be robustly inferred. We therefore propose a new genus for this species and rename it Divimonas melba n. gen., n. comb.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the Nature of the Peritrich Stalk: A Distinctive Organelle in a Large Group of Ciliated Unicellular Eukaryotes

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-09 DOI: 10.1111/jeu.70006

Chuanqi Jiang, Jing Zhang, Guangying Wang, Yuan Wang, Che Hu, Weiwei Qin, Tingting Pan, Siyu Gu, Xueyan Wang, Kai Chen, Xiaocui Chai, Mingkun Yang, Fang Zhou, Alan Warren, Jie Xiong, Wei Miao

{"title":"Decoding the Nature of the Peritrich Stalk: A Distinctive Organelle in a Large Group of Ciliated Unicellular Eukaryotes","authors":"Chuanqi Jiang, Jing Zhang, Guangying Wang, Yuan Wang, Che Hu, Weiwei Qin, Tingting Pan, Siyu Gu, Xueyan Wang, Kai Chen, Xiaocui Chai, Mingkun Yang, Fang Zhou, Alan Warren, Jie Xiong, Wei Miao","doi":"10.1111/jeu.70006","DOIUrl":"https://doi.org/10.1111/jeu.70006","url":null,"abstract":"<div>\u0000 \u0000 Ciliates represent a diverse assemblage of ancient single-celled eukaryotes characterized by diverse morphological features. Among certain sessilid peritrich ciliates, an exceptional morphological structure known as the stalk has been documented since the pioneering work of Antonie van Leeuwenhoek in the 17th century. This study conducts a comparative genomic analysis of three sessile peritrich species—Epistylis sp., Vorticella campanula, and Zoothamnium arbuscula—and two free-swimming species, Tetrahymena thermophila and Paramecium tetraurelia, within the class Oligohymenophorea. We find that carbohydrate-related components are consistently associated with diverse stalk substructures. Evidence suggests that the branched stalks of colonial E. hentscheli are supported by chitin-based ring-like structures. Through proteomic analysis of the Epistylis stalk, we found peritrich-specific genes, including coiled-coil domain-containing (CCDC) proteins and epidermal growth factor-like (EGF-like) proteins, as key stalk components. CCDC proteins are part of the stalk sheath, and their N-glycosylation may enhance adhesion between the cell body and stalk through lectin interactions. This study sheds light on the genetic innovations behind the stalk in peritrichs, which support their sessile and colonial lifestyles, and identifies peritrich-specific CCDC proteins as potential targets for disrupting the attachment of sessilids to aquaculture animals, addressing issues related to epibiotic burden.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and Molecular Phylogeny of Endosymbiotic Ciliates (Peritrichia, Mobilida) of Marine Invertebrates with Descriptions of Two Novel Species Urceolaria clepsydra n. sp. and Urceolaria bratalia n. sp. 海洋无脊椎动物内共生纤毛虫（蠕虫纲，游动纲）的形态学和分子系统发育以及两个新物种 Urceolaria clepsydra n. sp.

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-02-21 DOI: 10.1111/jeu.70003

Gabrielle Martinez, Brian S. Leander, Eunji Park

{"title":"Morphology and Molecular Phylogeny of Endosymbiotic Ciliates (Peritrichia, Mobilida) of Marine Invertebrates with Descriptions of Two Novel Species Urceolaria clepsydra n. sp. and Urceolaria bratalia n. sp.","authors":"Gabrielle Martinez, Brian S. Leander, Eunji Park","doi":"10.1111/jeu.70003","DOIUrl":"https://doi.org/10.1111/jeu.70003","url":null,"abstract":"Mobilid ciliates are a morphologically distinct group of protists that form a wide range of symbiotic relationships with aquatic animals and includes three subgroups: Trichodinidae, Urceolariidae, and Polycyclidae. Trichodinids are best known for infecting fishes, whereas urceolariids infect diverse marine invertebrates. Polycyclidae was established for mobilid ciliates infecting sea cucumbers; however, molecular data have been unavailable for this group. In this study, we discovered and characterized two novel mobilid species, one infecting two species of sea cucumbers (Eupentacta quinquesemita and Cucumaria miniata) and one infecting brachiopods or lamp shells (Terebratalia transversa) collected from the Northeast Pacific Ocean. These new mobilid species were characterized at the morphological level using light microscopy (LM) and scanning electron microscopy (SEM). We also inferred the molecular phylogenetic positions of these species using small subunit (SSU) rDNA sequences. Based on combined morphological and molecular data, we demonstrate that the two new species belong to Urceolaria, U. clepsydra n. sp. and U. bratalia n. sp., and support synonymization of Polycycla with Urceolaria. By providing the first molecular data from new species of mobilids infecting sea cucumbers and brachiopods, we expand the host range and improve our knowledge of this diverse but poorly understood group of symbionts.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0