Journal of Eukaryotic Microbiology最新文献

First Record of Aplanochytrium on the Chilean Coast and its Biotechnological Potential: Expanding the Geographic Distribution of Labyrinthulomycetes

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-16 DOI: 10.1111/jeu.70007

Pamela Jara-Zapata, Patricia Arancibia-Ávila, Moisés A. Valladares, Cristian Torres-Díaz, Edgar Pastene, Nicolás Reyes-Quinteros

{"title":"First Record of Aplanochytrium on the Chilean Coast and its Biotechnological Potential: Expanding the Geographic Distribution of Labyrinthulomycetes","authors":"Pamela Jara-Zapata, Patricia Arancibia-Ávila, Moisés A. Valladares, Cristian Torres-Díaz, Edgar Pastene, Nicolás Reyes-Quinteros","doi":"10.1111/jeu.70007","DOIUrl":"https://doi.org/10.1111/jeu.70007","url":null,"abstract":"<div>\u0000 \u0000 This study reports the first record of the genus Aplanochytrium on the coast of Chile, belonging to the class Labyrinthulomycetes in the kingdom Stramenopila. These marine protists play an essential role in carbon and nitrogen cycles as decomposers in the aquatic ecosystem. Although ecologically significant, their global diversity and distribution must be better understood. Samples were collected from Corral Bay, Valdivia, Chile, and later cultivated and identified through classical taxonomy and molecular analysis. Phylogenetic analysis based on the 18S rRNA sequence showed that Chilean samples were recovered in the Aplanochytrium s.s. lineage. Besides Aplanochytrium kerguelense Bahnweg & Sparrow, 1972, found in Kerguelen, an antarctic island from the Indian Ocean, this report extends the geographic distribution of the genus from Asia and the Northern Hemisphere to South America. This finding enhances our understanding of the diversity and distribution of Labyrinthulomycetes. Additionally, the fatty acid profile of Aplanochytrium revealed a high proportion of docosahexaenoic acid (DHA), accounting for 50% of the total fatty acids, along with the presence of palmitic acid. Astaxanthin, a valuable pigment for biotechnological applications, was also quantified. These results highlight the importance of Labyrinthulomycetes in marine ecosystems and their potential in biotechnology.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and molecular phylogeny of two soil species of Holostichides (Ciliophora, Hypotrichia) collected from eastern China, including a new species and a new subspecies

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-13 DOI: 10.1111/jeu.70001

Xuetong Zhao, Yaohan Jiang, Jie Huang, Xiaotian Luo, Yang Liu, Xiangrui Chen

{"title":"Morphology and molecular phylogeny of two soil species of Holostichides (Ciliophora, Hypotrichia) collected from eastern China, including a new species and a new subspecies","authors":"Xuetong Zhao, Yaohan Jiang, Jie Huang, Xiaotian Luo, Yang Liu, Xiangrui Chen","doi":"10.1111/jeu.70001","DOIUrl":"https://doi.org/10.1111/jeu.70001","url":null,"abstract":"Newly discovered hypotrich ciliates with evolutionary innovations are challenging to the established systematics and greater interest. Based on living morphology, infraciliature, and SSU rDNA, two bakuellid taxa—including a new species and a new subspecies—collected from subtropical soil environments in eastern China were investigated in the present study. Holostichides (Extraholostichides) eastensis ningboensis n. subsp. is characterized as follows: size in vivo 150–230 × 45–60 μm; cortical granules green and spherical; 3–5 small cirri arranged longitudinally posteriorly to middle frontal cirrus; row of 19–24 frontoterminal cirri exceeding half of the body length; 4–6 caudal cirri. Holostichides (Extraholostichides) muscus n. sp. is defined as follows: size in vivo 140–175 × 40–65 μm; cortical granules green and spherical; one or two small cirri posteriorly to the middle frontal cirrus; row of 3–6 frontoterminal cirri right of the midventral complex; three or four caudal cirri. Phylogenetic analyses based on SSU rDNA sequences revealed the systematic positions of these two new taxa and supported their validity as distinct subspecies and species, respectively.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validating the Genus Pocheina (Acrasidae, Heterolobosea, Discoba) Leads to the Recognition of Three Major Lineages Within Acrasidae

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-10 DOI: 10.1111/jeu.70004

Alexander K. Tice, Kevin Regis, Timothy E. Shutt, Frederick W. Spiegel, Matthew W. Brown, Jeffrey D. Silberman

{"title":"Validating the Genus Pocheina (Acrasidae, Heterolobosea, Discoba) Leads to the Recognition of Three Major Lineages Within Acrasidae","authors":"Alexander K. Tice, Kevin Regis, Timothy E. Shutt, Frederick W. Spiegel, Matthew W. Brown, Jeffrey D. Silberman","doi":"10.1111/jeu.70004","DOIUrl":"https://doi.org/10.1111/jeu.70004","url":null,"abstract":"<div>\u0000 \u0000 Pocheina and Acrasis are two genera of heterolobosean sorocarpic amoebae within Acrasidae that have historically been considered close relatives. The two genera were differentiated based on their differing fruiting body morphologies. The validity of this taxonomic distinction was challenged when a SSU rRNA phylogenetic study placed an isolate morphologically identified as “Pocheina” rosea within a clade of Acrasis rosea isolates. The authors speculated that pocheinoid fruiting body morphology might be the result of aberrant Ac. rosea fruiting body development, which, if true, would nullify this taxonomic distinction between genera. To clarify Acrasidae systematics, we analyzed SSU rRNA and ITS region sequences from multiple isolates of Pocheina, Acrasis, and Allovahlkampfia generated by Polymerase Chain Reaction (PCR) and transcriptomics. We demonstrate that the initial SSU sequence attributed to “P. rosea” originated from an Ac. rosea DNA contamination in its amplification reaction. Our analyses, based on morphology, SSU and 5.8S rRNA gene phylogenies, as well as comparative analyses of ITS1 and ITS2 sequences, resolve Acrasidae into three major lineages: Allovahlkampfia and the strongly supported clades comprising Pocheina and Acrasis. We confirm that the latter two genera can be identified by their fruiting body morphologies.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation of Ancyromonas melba, and Reclassification as the Type Species of Divimonas gen. nov., a Phylogenetically Important Ancyromonad Lineage

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-10 DOI: 10.1111/jeu.70005

Kate M. Barkhouse, Yana Eglit, Elizabeth J. Weston, Adriana B. A. Jenkins, Alastair G. B. Simpson

{"title":"Cultivation of Ancyromonas melba, and Reclassification as the Type Species of Divimonas gen. nov., a Phylogenetically Important Ancyromonad Lineage","authors":"Kate M. Barkhouse, Yana Eglit, Elizabeth J. Weston, Adriana B. A. Jenkins, Alastair G. B. Simpson","doi":"10.1111/jeu.70005","DOIUrl":"https://doi.org/10.1111/jeu.70005","url":null,"abstract":"Ancyromonadida is a taxon of small heterotrophic flagellates occupying an unresolved but deep-branching position in the eukaryotic tree of life, thus suspected to be important to studies of early eukaryotic evolutionary relationships and the characteristics of the last eukaryotic common ancestor. Sampling and cultivation of the full diversity of ancyromonad species are therefore areas of considerable interest. Ancyromonas melba is a species originally described from hypersaline material for which no monoprotistan culture or molecular data have been available, but whose distinct morphology suggests it may represent a new major lineage within Ancyromonadida. We report the first cultivation of this morphospecies in hypersaline media, with characterization including scanning electron microscopy and small subunit rRNA gene sequencing. Distinguishing morphological features include the predominantly ventral placement of the ventral groove, the approximately equal thickness of the anterior and posterior flagella, and the relatively long anterior flagellum. Phylogenetic analysis shows that the isolate does not branch within Ancyromonas or any other currently described genus of Ancyromonadida, but represents a novel genus-level lineage, the position of which within ancyromonads could not be robustly inferred. We therefore propose a new genus for this species and rename it Divimonas melba n. gen., n. comb.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the Nature of the Peritrich Stalk: A Distinctive Organelle in a Large Group of Ciliated Unicellular Eukaryotes

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-03-09 DOI: 10.1111/jeu.70006

Chuanqi Jiang, Jing Zhang, Guangying Wang, Yuan Wang, Che Hu, Weiwei Qin, Tingting Pan, Siyu Gu, Xueyan Wang, Kai Chen, Xiaocui Chai, Mingkun Yang, Fang Zhou, Alan Warren, Jie Xiong, Wei Miao

{"title":"Decoding the Nature of the Peritrich Stalk: A Distinctive Organelle in a Large Group of Ciliated Unicellular Eukaryotes","authors":"Chuanqi Jiang, Jing Zhang, Guangying Wang, Yuan Wang, Che Hu, Weiwei Qin, Tingting Pan, Siyu Gu, Xueyan Wang, Kai Chen, Xiaocui Chai, Mingkun Yang, Fang Zhou, Alan Warren, Jie Xiong, Wei Miao","doi":"10.1111/jeu.70006","DOIUrl":"https://doi.org/10.1111/jeu.70006","url":null,"abstract":"<div>\u0000 \u0000 Ciliates represent a diverse assemblage of ancient single-celled eukaryotes characterized by diverse morphological features. Among certain sessilid peritrich ciliates, an exceptional morphological structure known as the stalk has been documented since the pioneering work of Antonie van Leeuwenhoek in the 17th century. This study conducts a comparative genomic analysis of three sessile peritrich species—Epistylis sp., Vorticella campanula, and Zoothamnium arbuscula—and two free-swimming species, Tetrahymena thermophila and Paramecium tetraurelia, within the class Oligohymenophorea. We find that carbohydrate-related components are consistently associated with diverse stalk substructures. Evidence suggests that the branched stalks of colonial E. hentscheli are supported by chitin-based ring-like structures. Through proteomic analysis of the Epistylis stalk, we found peritrich-specific genes, including coiled-coil domain-containing (CCDC) proteins and epidermal growth factor-like (EGF-like) proteins, as key stalk components. CCDC proteins are part of the stalk sheath, and their N-glycosylation may enhance adhesion between the cell body and stalk through lectin interactions. This study sheds light on the genetic innovations behind the stalk in peritrichs, which support their sessile and colonial lifestyles, and identifies peritrich-specific CCDC proteins as potential targets for disrupting the attachment of sessilids to aquaculture animals, addressing issues related to epibiotic burden.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and Molecular Phylogeny of Endosymbiotic Ciliates (Peritrichia, Mobilida) of Marine Invertebrates with Descriptions of Two Novel Species Urceolaria clepsydra n. sp. and Urceolaria bratalia n. sp. 海洋无脊椎动物内共生纤毛虫（蠕虫纲，游动纲）的形态学和分子系统发育以及两个新物种 Urceolaria clepsydra n. sp.

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-02-21 DOI: 10.1111/jeu.70003

Gabrielle Martinez, Brian S. Leander, Eunji Park

{"title":"Morphology and Molecular Phylogeny of Endosymbiotic Ciliates (Peritrichia, Mobilida) of Marine Invertebrates with Descriptions of Two Novel Species Urceolaria clepsydra n. sp. and Urceolaria bratalia n. sp.","authors":"Gabrielle Martinez, Brian S. Leander, Eunji Park","doi":"10.1111/jeu.70003","DOIUrl":"https://doi.org/10.1111/jeu.70003","url":null,"abstract":"Mobilid ciliates are a morphologically distinct group of protists that form a wide range of symbiotic relationships with aquatic animals and includes three subgroups: Trichodinidae, Urceolariidae, and Polycyclidae. Trichodinids are best known for infecting fishes, whereas urceolariids infect diverse marine invertebrates. Polycyclidae was established for mobilid ciliates infecting sea cucumbers; however, molecular data have been unavailable for this group. In this study, we discovered and characterized two novel mobilid species, one infecting two species of sea cucumbers (Eupentacta quinquesemita and Cucumaria miniata) and one infecting brachiopods or lamp shells (Terebratalia transversa) collected from the Northeast Pacific Ocean. These new mobilid species were characterized at the morphological level using light microscopy (LM) and scanning electron microscopy (SEM). We also inferred the molecular phylogenetic positions of these species using small subunit (SSU) rDNA sequences. Based on combined morphological and molecular data, we demonstrate that the two new species belong to Urceolaria, U. clepsydra n. sp. and U. bratalia n. sp., and support synonymization of Polycycla with Urceolaria. By providing the first molecular data from new species of mobilids infecting sea cucumbers and brachiopods, we expand the host range and improve our knowledge of this diverse but poorly understood group of symbionts.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Molecular Phylogenetic Characterization of Three New Marine Goniomonad Species

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-02-21 DOI: 10.1111/jeu.70002

Yasinee Phanprasert, Sun Young Kim, Nam Seon Kang, Minseok Jeong, Jong Im Kim, Woongghi Shin, Won Je Lee, Eunsoo Kim

{"title":"Morphological and Molecular Phylogenetic Characterization of Three New Marine Goniomonad Species","authors":"Yasinee Phanprasert, Sun Young Kim, Nam Seon Kang, Minseok Jeong, Jong Im Kim, Woongghi Shin, Won Je Lee, Eunsoo Kim","doi":"10.1111/jeu.70002","DOIUrl":"https://doi.org/10.1111/jeu.70002","url":null,"abstract":"<div>\u0000 \u0000 Goniomonads are commonly found heterotrophic biflagellates in both marine and freshwater environments. Despite the high genetic diversity inferred from 18S rDNA data, many goniomonad species remain undescribed. In this study, we established a total of 21 marine goniomonad culture strains, and from these, describe three new species by using 18S rDNA phylogeny, light microscopy, and electron microscopy. Molecular sequence analyses suggest the presence of several distinct sub-lineages within the marine goniomonad clade. Two of these are Goniomonas ulleungensis sp. nov. and G. lingua sp. nov., which are similar in size, flagellar length, appendage, and orientation and have a tongue-like protrusion at the anterior. The two species can be differentiated by the periplast plate pattern with G. ulleungensis displaying one additional plate on the right side. G. duplex sp. nov. differed from these two species by having two unequal flagella with the longer one trailing posteriorly and having the opposite cell orientation when skidding. Comparative analyses of five marine goniomonad species showed that genetically distinct goniomonad groups can be delineated by morphological data as well, and of several morphological features that are of taxonomic utility, the periplast plate pattern, observable by SEM, is particularly informative in goniomonad taxonomy.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenicity and phylogeny of Labyrinthula spp. isolated in Washington and Oregon, USA

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-01-27 DOI: 10.1111/jeu.13073

M. Victoria Agnew-Camiener, Morgan E. Eisenlord, Carolyn S. Friedman, Harold J. Schreier, Colleen A. Burge

{"title":"Pathogenicity and phylogeny of Labyrinthula spp. isolated in Washington and Oregon, USA","authors":"M. Victoria Agnew-Camiener, Morgan E. Eisenlord, Carolyn S. Friedman, Harold J. Schreier, Colleen A. Burge","doi":"10.1111/jeu.13073","DOIUrl":"10.1111/jeu.13073","url":null,"abstract":"The class Labyrinthulomycetes constitutes a multitude of species found ubiquitously in the environment, and includes pathogens of corals, hard clams, turfgrasses, and seagrasses. Labyrinthula zosterae, the causative agent of seagrass wasting disease, has been associated with declines in seagrass coverage since the 1930s. However, pathogenic and nonpathogenic Labyrinthula spp. have been isolated from seagrass tissue. These isolates are difficult to distinguish morphologically, and the diversity of isolates where seagrass wasting disease is present is often unknown. This study aimed to increase knowledge on the pathogenicity and phylogeny of Labyrinthula spp. in Washington and Oregon, USA where a high prevalence of seagrass wasting disease has been associated with eelgrass, Zostera marina, declines. We tested the pathogenicity of 14 Labyrinthula isolates and compared partial 18S rRNA gene sequences of 12 isolates to sequences from around the world through the NCBI database. We found that pathogenic isolates could be identified as Labyrinthula zosterae, while nonpathogenic isolates did not form a clade with any previously identified SSU ribotypes. These results add to the growing data on Labyrinthula and seagrass wasting disease and can improve our understanding of pathogen evolution and spread in the future.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The planktonic freshwater ciliate Balanion planctonicum (Ciliophora, Prostomatea): A cryptic species complex or a “complex species”?

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-01-27 DOI: 10.1111/jeu.13084

Martina Schalch-Schuler, Barbara Bassin, Adrian-Stefan Andrei, Gianna Dirren-Pitsch, Katherine Waller, Cyrill Hofer, Jakob Pernthaler, Thomas Posch

{"title":"The planktonic freshwater ciliate Balanion planctonicum (Ciliophora, Prostomatea): A cryptic species complex or a “complex species”?","authors":"Martina Schalch-Schuler, Barbara Bassin, Adrian-Stefan Andrei, Gianna Dirren-Pitsch, Katherine Waller, Cyrill Hofer, Jakob Pernthaler, Thomas Posch","doi":"10.1111/jeu.13084","DOIUrl":"10.1111/jeu.13084","url":null,"abstract":"The globally distributed ciliate Balanion planctonicum is a primary consumer of phytoplankton spring blooms. Due to its small size (~20 μm), identification and quantification by molecular tools is preferable as an alternative to the laborious counting of specimen in quantitative protargol stains. However, previous sequencing of the 18S rDNA V9 region of B. planctonicum from Lake Zurich (Switzerland) and subsequent quantification by fluorescence in situ hybridization yielded significantly lower cell numbers than using morphotype counting. This raised the question of whether B. planctonicum shows a cryptic diversity or whether it is just a ‘complex species’ with intra-clonal polymorphisms. Over three years, we established numerous monoclonal cultures, and long-read sequencing of rDNA operons revealed four distinct dominant haplotypes (BpHs 1–4). The gene sequences of BpHs 1 and 3 differed by 6% and did not share intra-clonal polymorphisms, providing evidence for two distinct clades. Furthermore, phylogenetic analyses corroborate the sister relationship between Balanion and Askenasia (plus Hexasterias and Radiosperma). Morphologically, the two Balanion clades are nearly indistinguishable with small differences in macronucleus size and in the cell length to width ratio. CARD-FISH analyses indicated that the diversity of B. planctonicum is even more extensive with still unidentified clades.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Allobodo yubaba sp. nov. and Novijibodo darinka gen. et sp. nov., cultivable free-living species of the phylogenetically enigmatic kinetoplastid taxon Allobodonidae

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-01-27 DOI: 10.1111/jeu.13072

Julia A. Packer, Daryna Zavadska, Elizabeth J. Weston, Yana Eglit, Daniel J. Richter, Alastair G. B. Simpson

{"title":"Characterization of Allobodo yubaba sp. nov. and Novijibodo darinka gen. et sp. nov., cultivable free-living species of the phylogenetically enigmatic kinetoplastid taxon Allobodonidae","authors":"Julia A. Packer, Daryna Zavadska, Elizabeth J. Weston, Yana Eglit, Daniel J. Richter, Alastair G. B. Simpson","doi":"10.1111/jeu.13072","DOIUrl":"10.1111/jeu.13072","url":null,"abstract":"Kinetoplastids are a large and diverse protist group, spanning ecologically important free-living forms to medically important parasites. The taxon Allobodonidae holds an unresolved position within kinetoplastids, and the sole described species, Allobodo chlorophagus, is uncultivated, being a necrotroph/parasite of macroalgae. Here we describe Allobodo yubaba sp. nov. and Novijibodo darinka gen. nov. et sp. nov., both free-living bacterivores isolated into monoeukaryotic cultures. Electron microscopy shows that both A. yubaba and N. darinka have a microtubular prism in the feeding apparatus (absent in A. chlorophagus), and an ovoid eukinetoplast, rather than pan-kDNA as in A. chlorophagus. Phylogenetic analyses of SSU rDNA sequences robustly place A. yubaba as the sister to A. chlorophagus, while N. darinka branches separately within Allobodonidae, as a sister group of undescribed freshwater isolates. We view Allobodonidae as containing at least four genus-level clades: Allobodo (A. chlorophagus and A. yubaba n. sp.), an undescribed fresh-water clade, an undescribed marine clade, and now Novijibodo—with N. darinka as its sole known member. Electron microscopy also revealed a rod-shaped gram-negative bacterial cytoplasmic endosymbiont in our N. darinka isolate. The availability of these species in monoeukaryotic culture should facilitate future research, including resolving the position of Allobodonidae using phylogenomic approaches.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0