Journal of Eukaryotic Microbiology最新文献

Metabolic Profile Associated With Encystation in Acanthamoeba 棘阿米巴虫体内与成囊有关的代谢谱

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-30 DOI: 10.1111/jeu.70034

Cecília Cirelli, Isabela Aurora Rodrigues, Jéssica Gardone Vitório, Filipe Fideles Duarte-Andrade, Gisele André Baptista Canuto, Leiliane Coelho André, Juliano Simões de Toledo, Ana Paula Fernandes, Adriana Oliveira Costa

{"title":"Metabolic Profile Associated With Encystation in Acanthamoeba","authors":"Cecília Cirelli, Isabela Aurora Rodrigues, Jéssica Gardone Vitório, Filipe Fideles Duarte-Andrade, Gisele André Baptista Canuto, Leiliane Coelho André, Juliano Simões de Toledo, Ana Paula Fernandes, Adriana Oliveira Costa","doi":"10.1111/jeu.70034","DOIUrl":"https://doi.org/10.1111/jeu.70034","url":null,"abstract":"The genus Acanthamoeba includes widespread protozoa that can cause severe infections in humans. Their ability to form resistant cysts within infected tissues complicates treatment, making it essential to understand the encystation process for developing effective therapeutic strategies. This study utilized untargeted metabolomics (GC–MS) to analyze metabolic changes during the encystation of an Acanthamoeba strain in Neff's encystation saline. We conducted metabolite analysis at three stages of differentiation: the trophozoite-dominant phase (0 h), the pre-cyst-dominant phase (24 h), and the cyst-dominant phase (72 h). The results indicated a global metabolic downregulation during encystation, which is consistent with a state of dormancy. Components of the cyst wall such as cellobiose and lactose accumulated in the final phase. Arbutin and canavanine were annotated for the first time in Acanthamoeba. Encystation also led to changes in pathways related to glycine, serine, and threonine metabolism and biosynthesis of aminoacyl-tRNA. This study uncovered previously unknown metabolites and metabolic pathways at distinct stages of Acanthamoeba development.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructure of the Endoplasmic Reticulum in Eukaryotic Microalgae 真核微藻内质网的超微结构

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-30 DOI: 10.1111/jeu.70030

Ursula Goodenough, Robyn Roth

{"title":"Ultrastructure of the Endoplasmic Reticulum in Eukaryotic Microalgae","authors":"Ursula Goodenough, Robyn Roth","doi":"10.1111/jeu.70030","DOIUrl":"https://doi.org/10.1111/jeu.70030","url":null,"abstract":"The endoplasmic reticulum (ER) is a large and highly dynamic component of the eukaryotic endomembrane system. In eukaryotic microalgae, it plays six distinct roles: (1) It envelopes the chromatin to form the nucleus. (2) It forms cisternae in the cytoplasm, some of which scaffold the synthesis of proteins destined for incorporation into membranes or for secretion. (3) It associates with Golgi cisternae to scaffold the synthesis of glycosylated proteins. (4) It associates with the plasma membrane to mediate the synthesis and secretion of hydrophobic molecules. (5) It mediates the synthesis of cytoplasmic lipid bodies. (6) In lineages harboring complex plastids of red algal ancestry, it forms the chloroplast ER, which envelops the primary chloroplast envelope. In this review, these systems are illustrated using the quick-freeze deep-etch electron microscopy (QFDEEM) technique, which lifts up the topological configurations adopted by this gossamer system. A key finding is that in all the complex microalgae examined except dinoflagellates, the inner nuclear envelope membrane associates directly with the plastid-contiguous membrane of the chloroplast ER at foci designated as chloroplast-nuclear junctions. These junctions may play a role in regulating the maintenance and physiology of the complex organelles.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the Diversity of Pathogenic Free-Living Amoebae in Freshwater Environments of Ghana: A Combined Culture Enrichment and Metabarcoding Approach 加纳淡水环境中病原自由生活变形虫多样性的揭示：一种培养富集和元条形码结合的方法

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-20 DOI: 10.1111/jeu.70032

Yonas I. Tekle, Kwaku Oti Acheampong, Richard Kwame Adu, Kwaku Brako Dakwa

{"title":"Uncovering the Diversity of Pathogenic Free-Living Amoebae in Freshwater Environments of Ghana: A Combined Culture Enrichment and Metabarcoding Approach","authors":"Yonas I. Tekle, Kwaku Oti Acheampong, Richard Kwame Adu, Kwaku Brako Dakwa","doi":"10.1111/jeu.70032","DOIUrl":"https://doi.org/10.1111/jeu.70032","url":null,"abstract":"<div>\u0000 \u0000 Free-living amoebae (FLA) such as Naegleria and Acanthamoeba are opportunistic pathogens increasingly linked to fatal and severe human infections, particularly in settings with limited hygiene, water sanitation, and diagnostic infrastructure. In this pilot study, we explored the diversity of potentially pathogenic FLA in sectors of the Kakum River Basin near Cape Coast, Ghana, using a combined approach of filtered, pelleted, and culture-enriched metabarcoding. Our results revealed a wide range of FLA from Amoebozoa and Heterolobosea clades, including several of clinical relevance, such as Acanthamoeba, Vermamoeba, Balamuthia, and Paravahlkampfia. Importantly, various FLA, including Naegleria and most of the opportunistic amoebae species, were also detected and morphologically confirmed. This raises public health concerns, given the favorable environmental conditions for their proliferation in the sampling sites. The use of culture-enriched metabarcoding was particularly valuable in recovering organisms that may encyst and be missed by direct methods. This study underscores the importance of integrative and sensitive molecular approaches for detecting neglected pathogens in vulnerable communities. Our findings provide a foundation for larger epidemiological studies that incorporate clinical data and support the development of cost-effective monitoring tools for FLA-associated public health threats in sub-Saharan Africa.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Electron Microscopy Reveals the Structural Complexity of the Intravacuolar Membranous Network in Cyrilia lignieresi-Infected Erythrocytes of the Fish Synbranchus marmoratus 三维电子显微镜揭示了木质素西霉感染的马氏合鳃鱼红细胞的孔内膜网络结构的复杂性

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-20 DOI: 10.1111/jeu.70031

Brenda Santarém Fachetii, Maíra Turiel-Silva, Camila Wendt, Hilton Tulio Costi, Edilene Oliveira da Silva, Ana Paula Drummond Rodrigues, Wanderley de Souza, Kildare Miranda, José Antonio Picanço Diniz

{"title":"3D Electron Microscopy Reveals the Structural Complexity of the Intravacuolar Membranous Network in Cyrilia lignieresi-Infected Erythrocytes of the Fish Synbranchus marmoratus","authors":"Brenda Santarém Fachetii, Maíra Turiel-Silva, Camila Wendt, Hilton Tulio Costi, Edilene Oliveira da Silva, Ana Paula Drummond Rodrigues, Wanderley de Souza, Kildare Miranda, José Antonio Picanço Diniz","doi":"10.1111/jeu.70031","DOIUrl":"https://doi.org/10.1111/jeu.70031","url":null,"abstract":"This study employs advanced three-dimensional electron microscopy techniques, including Transmission Electron Microscopy (TEM) tomography and freeze-fracture imaging via scanning electron microscopy (SEM), to investigate the ultrastructural organization of Cyrilia lignieresi-infected red blood cells (iRBCs) in the host fish Synbranchus marmoratus. The analysis focuses on the parasitophorous vacuole (PV) and reveals a highly complex intravacuolar membranous network (IVN) composed of vesicles, tubules, and interconnected membranous structures. These elements exhibit considerable diversity in size, morphology, and electron density, suggesting dynamic functional roles in the parasite–host interaction. The electron tomography and three-dimensional reconstructions data provide unprecedented insights into the spatial organization and potential functional significance of these membranous systems. These findings not only enhance our understanding of the cellular adaptations of C. lignieresi but also contribute to a broader knowledge of apicomplexan parasitism and host–pathogen interactions.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host Organelle Interactions Facilitate Cholesterol Acquisition by Trypanosoma cruzi Amastigotes 宿主细胞器相互作用促进克氏锥虫无轴虫获得胆固醇

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-20 DOI: 10.1111/jeu.70027

Carolina de Lima Alcantara, Miria Gomes Pereira, Wanderley de Souza, Narcisa Leal da Cunha-e-Silva

{"title":"Host Organelle Interactions Facilitate Cholesterol Acquisition by Trypanosoma cruzi Amastigotes","authors":"Carolina de Lima Alcantara, Miria Gomes Pereira, Wanderley de Souza, Narcisa Leal da Cunha-e-Silva","doi":"10.1111/jeu.70027","DOIUrl":"https://doi.org/10.1111/jeu.70027","url":null,"abstract":"Chagas disease, caused by the protozoan Trypanosoma cruzi, is a major neglected disease in Latin America. The amastigote, the replicative intracellular form, is essential for infection persistence in vertebrate hosts. These forms exhibit remarkable adaptability, modulating metabolism and growth according to host cell resource availability. Lipid metabolism plays a critical role in amastigote development, with a strong dependence on host-derived lipids, particularly cholesterol. Although T. cruzi can synthesize some sterols and fatty acids, it also scavenges essential lipids from the host. Changes in host cholesterol metabolism, possibly via SREBPs, may increase intracellular cholesterol levels and promote parasite growth. However, the mechanisms of cholesterol acquisition by amastigotes remain unclear. Here, we investigated cholesterol trafficking from host cells to amastigotes using a fluorescent cholesterol analog. Through confocal and volume electron microscopy, we demonstrated cholesterol uptake by amastigotes, characterized uptake kinetics, and confirmed its importance for parasite development. We also revealed close contact between the host endoplasmic reticulum and the amastigote plasma membrane, consistent with membrane contact sites. Furthermore, we showed that amastigotes can internalize ER- and Golgi-derived host markers, suggesting a potential route for acquisition of host molecules. These findings provide new insights into lipid acquisition strategies by intracellular T. cruzi amastigotes.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to Morphology, Systematics and Life Cycle of Ozanamia fimbriatus (Haptista: Centroplasthelida), With Notes on Evolution of Organic Skeleton in Centrohelids 毛蕊虫形态、分类学和生活史的修正——附关于中心体有机骨架演化的注解

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-16 DOI: 10.1111/jeu.70029

引用次数: 0

Experimental Evidence for Conservation of the Ubiquitin-Like Autophagy-Related ATG12 Conjugation System in Trichomonas vaginalis 阴道毛滴虫泛素样自噬相关ATG12偶联系统保存的实验证据

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-15 DOI: 10.1111/jeu.70028

Xiaoxia X. Lin, Yumiko Saito-Nakano, Euki Yazaki, Ryo Harada, Dingming V. Zhao, Kenji Hikosaka, Hirokazu Sakamoto

引用次数: 0

Report of the 44th Annual Meeting of the Society of Eukaryotic Microbiology (EuMik) 第44届真核微生物学会年会报告

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-09 DOI: 10.1111/jeu.70024

Edward A. D. Mitchell

引用次数: 0

How Single Cells Form Shells: Maturation and Secretion of Lorica-Forming Material in the Tintinnid Schmidingerella (Alveolata, Ciliophora) 单细胞是如何形成壳的：丁丁属Schmidingerella （Alveolata, Ciliophora）中lorica形成物质的成熟和分泌

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-08 DOI: 10.1111/jeu.70025

Maximilian H. Ganser, Birgit Weißenbacher, Sabine Agatha

{"title":"How Single Cells Form Shells: Maturation and Secretion of Lorica-Forming Material in the Tintinnid Schmidingerella (Alveolata, Ciliophora)","authors":"Maximilian H. Ganser, Birgit Weißenbacher, Sabine Agatha","doi":"10.1111/jeu.70025","DOIUrl":"https://doi.org/10.1111/jeu.70025","url":null,"abstract":"Tintinnid ciliates are distinguished by their loricae (shells), the key synapomorphy of these mainly marine planktonic protists. They can divide daily, with a considerable portion of biomass stored in the loricae. During each division, lorica-forming material (LFM) is produced and afterwards used by the proter (anterior division product) to construct a new lorica, while the opisthe (posterior division product) retains the parental one. Many aspects of lorica formation remain unclear, and no study describes the entire process from material maturation via secretion to assembly. Here, we present the first thorough investigation at cellular and sub-cellular levels, employing light microscopy on dividers and postdividers, as well as transmission electron microscopy on primarily cryofixed specimens from a Schmidingerella culture. Our study reconstructs LFM maturation, identifying two main developmental stages: the morula-shaped precursor granules and the mature granules. The latter cluster in the proter's ventral portion with a peripheral longitudinal strip of small granules embedded in large ones. Ultrastructurally and chemically, the mature granules of both size classes are identical. Through detailed live observations, we followed and documented, for the first time, the process of cell division, the behavior of the proter, the release of LFM granules, and features of lorica formation.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large Genomes Are Associated With Greater Cell Size and Ecological Shift Towards More Nitrogen-Rich and Higher-Latitude Environments in Microalgae of the Genus Synura 大基因组与大细胞大小和向更富氮和高纬度环境的生态转移有关

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-02 DOI: 10.1111/jeu.70026

Dora Čertnerová, Pavel Škaloud, Iva Jadrná, Martin Čertner

{"title":"Large Genomes Are Associated With Greater Cell Size and Ecological Shift Towards More Nitrogen-Rich and Higher-Latitude Environments in Microalgae of the Genus Synura","authors":"Dora Čertnerová, Pavel Škaloud, Iva Jadrná, Martin Čertner","doi":"10.1111/jeu.70026","DOIUrl":"https://doi.org/10.1111/jeu.70026","url":null,"abstract":"The nuclear genome is essential for encoding most of the genes required for cellular processes, but its size alone can alter the characteristics of cells and organisms. Yet, genome size variation and its ecological and evolutionary impacts, particularly in microorganisms, are not well understood. We used flow cytometry to estimate genome size and GC content in 53 evolutionary lineages of the microalgal genus Synura (Chrysophyceae, Stramenopiles). Genome size evolution was reconstructed in a phylogenetic framework using molecular markers. A set of genomic, morphological, and ecogeographic variables characterizing Synura lineages was evaluated and tested as predictors of genome size variation in phylogeny-corrected statistical models. Both genome size and GC content varied widely in Synura, ranging from 0.19 to 3.70 pg of DNA and 34.0% to 49.3%, respectively. Genome size variation was mainly associated with cell size, less with silica scale size, and not with scale ultrastructure. Higher soil nitrogen, higher latitudes, and lower temperatures correlated with larger genomes. Genome size evolution in Synura shows potential dynamism, with increases confined to short terminal branches, indicating lower macroevolutionary stability. Lineages with larger genomes exhibited a narrower range of suitable ecological conditions, possibly due to selection acting deleteriously against larger genomes (and cells).","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0