Journal of Eukaryotic Microbiology最新文献_第2页

Correction to 3D Electron Microscopy Reveals the Structural Complexity of the Intravacuolar Membranous Network in Cyrilia lignieresi-Infected Erythrocytes of the Fish Synbranchus marmoratus 校正三维电子显微镜揭示了斑马鱼合鳃红细胞中木质素西霉感染的孔内膜网络结构的复杂性

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-08-18 DOI: 10.1111/jeu.70036

引用次数: 0

Intragenomic 18S Sequence Variability in the Termite Symbionts Kofoidia loriculata and Kofoidia repleta n. sp. (Parabasalia) 白蚁共生体loriculata和Kofoidia repleta n. sp. （Parabasalia）的18S序列基因组内变异

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-08-13 DOI: 10.1111/jeu.70039

Ezhan Mirza, Daniel E. Jasso-Selles, Nobuaki Mizumoto, Gillian H. Gile

引用次数: 0

Bioprospecting of Chilean Labyrinthulomycetes: Laboratory Evaluation of PUFA and Carotenoid Production in Isolates From Diverse Natural Environments 智利迷路菌的生物勘探：不同自然环境分离菌PUFA和类胡萝卜素产量的实验室评价

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-08-12 DOI: 10.1111/jeu.70035

Pamela Jara-Zapata, Patricia Arancibia-Ávila, Moisés A. Valladares, Cristian Torres-Díaz, Edgar Pastene, Nicolás Reyes-Quinteros

{"title":"Bioprospecting of Chilean Labyrinthulomycetes: Laboratory Evaluation of PUFA and Carotenoid Production in Isolates From Diverse Natural Environments","authors":"Pamela Jara-Zapata, Patricia Arancibia-Ávila, Moisés A. Valladares, Cristian Torres-Díaz, Edgar Pastene, Nicolás Reyes-Quinteros","doi":"10.1111/jeu.70035","DOIUrl":"https://doi.org/10.1111/jeu.70035","url":null,"abstract":"<div>\u0000 \u0000 This study examines the production of polyunsaturated fatty acids (PUFAs) and carotenoids in Labyrinthulomycetes isolated along a thermal gradient on the Chilean coast. Phylogenetic analysis revealed clades corresponding to Oblongichytrium, Schizochytrium, and Thraustochytrium aggregatum. Strains of Oblongichytrium sp. from colder, higher pH sites (9°C, pH 8.64) produced up to 34 times more DHA in laboratory conditions than those from warmer, lower pH areas (13°C, pH 7.30), suggesting an influence of native environmental conditions on biosynthetic capacity. In contrast, DHA production by Schizochytrium sp. and T. aggregatum was limited to specific isolates, without a clear latitudinal pattern. Polyunsaturated fatty acids were detected in all strains, although no relationship with environmental variables was found. All strains produced astaxanthin, while β-carotene was detected in some, with no consistent pattern; canthaxanthin was absent. The results highlight the high biotechnological potential of Oblongichytrium strains from colder, alkaline coastal environments, emphasizing the importance of exploring diverse natural habitats for organisms with valuable lipid production profiles. This study supports the value of targeted bioprospecting to identify strains with promising applications in lipid-based industries.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organellar Genomes of Three Globally Important Nanoplanktonic Diatoms Refine Their Taxon-Specific Distribution and Succession Patterns in the Northwest Atlantic 西北大西洋三种全球重要的纳米浮游硅藻的细胞器基因组改进了它们的分类群特定分布和演替模式

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-08-06 DOI: 10.1111/jeu.70033

Rebecca Stevens-Green, Caroline Chénard, Solenn Mordret, Jenna MacKinnon, Brent M. Robicheau, Julie LaRoche

{"title":"Organellar Genomes of Three Globally Important Nanoplanktonic Diatoms Refine Their Taxon-Specific Distribution and Succession Patterns in the Northwest Atlantic","authors":"Rebecca Stevens-Green, Caroline Chénard, Solenn Mordret, Jenna MacKinnon, Brent M. Robicheau, Julie LaRoche","doi":"10.1111/jeu.70033","DOIUrl":"https://doi.org/10.1111/jeu.70033","url":null,"abstract":"Nanoplanktonic diatoms (2–20 μm) are a significant yet historically understudied component of marine ecosystems. We investigated three recently isolated nanoplanktonic diatoms from the Northwest Atlantic Ocean (NWA): Minidiscus spinulatus, Mediolabrus comicus, and Minidiscus trioculatus. Using Oxford Nanopore sequencing, we assembled and annotated their complete chloroplast and mitochondrial genomes. Pangenome analyses revealed that Minidiscus species consistently clustered more closely with select Thalassiosira species, whereas M. comicus formed a sister clade with Skeletonema. Circularized chloroplast genomes allowed us to characterize the full-length 16S ribosomal RNAs for each isolate, thereby leading to higher resolution of these taxa in preexisting 16S metabarcoding data. During our study, M. spinulatus was primarily restricted to the Bedford Basin. In contrast, both M. trioculatus and M. comicus had larger geographic ranges extending to the Labrador Sea, and in the case of M. comicus, to the Canadian Arctic Gateway. Weekly metabarcoding from the coastal Bedford Basin, N.S., Canada (2014–2022), revealed a seasonal succession of nanoplanktonic taxa, with Minidiscus trioculatus dominating in the early months, followed by M. comicus and M. spinulatus. Our results highlight the critical value of phytoplankton isolations and organelle genomics for expanding our understanding of the diversity and biogeography of nanoplanktonic diatoms.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Profile Associated With Encystation in Acanthamoeba 棘阿米巴虫体内与成囊有关的代谢谱

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-30 DOI: 10.1111/jeu.70034

Cecília Cirelli, Isabela Aurora Rodrigues, Jéssica Gardone Vitório, Filipe Fideles Duarte-Andrade, Gisele André Baptista Canuto, Leiliane Coelho André, Juliano Simões de Toledo, Ana Paula Fernandes, Adriana Oliveira Costa

{"title":"Metabolic Profile Associated With Encystation in Acanthamoeba","authors":"Cecília Cirelli, Isabela Aurora Rodrigues, Jéssica Gardone Vitório, Filipe Fideles Duarte-Andrade, Gisele André Baptista Canuto, Leiliane Coelho André, Juliano Simões de Toledo, Ana Paula Fernandes, Adriana Oliveira Costa","doi":"10.1111/jeu.70034","DOIUrl":"https://doi.org/10.1111/jeu.70034","url":null,"abstract":"The genus Acanthamoeba includes widespread protozoa that can cause severe infections in humans. Their ability to form resistant cysts within infected tissues complicates treatment, making it essential to understand the encystation process for developing effective therapeutic strategies. This study utilized untargeted metabolomics (GC–MS) to analyze metabolic changes during the encystation of an Acanthamoeba strain in Neff's encystation saline. We conducted metabolite analysis at three stages of differentiation: the trophozoite-dominant phase (0 h), the pre-cyst-dominant phase (24 h), and the cyst-dominant phase (72 h). The results indicated a global metabolic downregulation during encystation, which is consistent with a state of dormancy. Components of the cyst wall such as cellobiose and lactose accumulated in the final phase. Arbutin and canavanine were annotated for the first time in Acanthamoeba. Encystation also led to changes in pathways related to glycine, serine, and threonine metabolism and biosynthesis of aminoacyl-tRNA. This study uncovered previously unknown metabolites and metabolic pathways at distinct stages of Acanthamoeba development.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructure of the Endoplasmic Reticulum in Eukaryotic Microalgae 真核微藻内质网的超微结构

IF 2.6 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-30 DOI: 10.1111/jeu.70030

Ursula Goodenough, Robyn Roth

{"title":"Ultrastructure of the Endoplasmic Reticulum in Eukaryotic Microalgae","authors":"Ursula Goodenough, Robyn Roth","doi":"10.1111/jeu.70030","DOIUrl":"https://doi.org/10.1111/jeu.70030","url":null,"abstract":"The endoplasmic reticulum (ER) is a large and highly dynamic component of the eukaryotic endomembrane system. In eukaryotic microalgae, it plays six distinct roles: (1) It envelopes the chromatin to form the nucleus. (2) It forms cisternae in the cytoplasm, some of which scaffold the synthesis of proteins destined for incorporation into membranes or for secretion. (3) It associates with Golgi cisternae to scaffold the synthesis of glycosylated proteins. (4) It associates with the plasma membrane to mediate the synthesis and secretion of hydrophobic molecules. (5) It mediates the synthesis of cytoplasmic lipid bodies. (6) In lineages harboring complex plastids of red algal ancestry, it forms the chloroplast ER, which envelops the primary chloroplast envelope. In this review, these systems are illustrated using the quick-freeze deep-etch electron microscopy (QFDEEM) technique, which lifts up the topological configurations adopted by this gossamer system. A key finding is that in all the complex microalgae examined except dinoflagellates, the inner nuclear envelope membrane associates directly with the plastid-contiguous membrane of the chloroplast ER at foci designated as chloroplast-nuclear junctions. These junctions may play a role in regulating the maintenance and physiology of the complex organelles.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the Diversity of Pathogenic Free-Living Amoebae in Freshwater Environments of Ghana: A Combined Culture Enrichment and Metabarcoding Approach 加纳淡水环境中病原自由生活变形虫多样性的揭示：一种培养富集和元条形码结合的方法

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-20 DOI: 10.1111/jeu.70032

Yonas I. Tekle, Kwaku Oti Acheampong, Richard Kwame Adu, Kwaku Brako Dakwa

{"title":"Uncovering the Diversity of Pathogenic Free-Living Amoebae in Freshwater Environments of Ghana: A Combined Culture Enrichment and Metabarcoding Approach","authors":"Yonas I. Tekle, Kwaku Oti Acheampong, Richard Kwame Adu, Kwaku Brako Dakwa","doi":"10.1111/jeu.70032","DOIUrl":"https://doi.org/10.1111/jeu.70032","url":null,"abstract":"<div>\u0000 \u0000 Free-living amoebae (FLA) such as Naegleria and Acanthamoeba are opportunistic pathogens increasingly linked to fatal and severe human infections, particularly in settings with limited hygiene, water sanitation, and diagnostic infrastructure. In this pilot study, we explored the diversity of potentially pathogenic FLA in sectors of the Kakum River Basin near Cape Coast, Ghana, using a combined approach of filtered, pelleted, and culture-enriched metabarcoding. Our results revealed a wide range of FLA from Amoebozoa and Heterolobosea clades, including several of clinical relevance, such as Acanthamoeba, Vermamoeba, Balamuthia, and Paravahlkampfia. Importantly, various FLA, including Naegleria and most of the opportunistic amoebae species, were also detected and morphologically confirmed. This raises public health concerns, given the favorable environmental conditions for their proliferation in the sampling sites. The use of culture-enriched metabarcoding was particularly valuable in recovering organisms that may encyst and be missed by direct methods. This study underscores the importance of integrative and sensitive molecular approaches for detecting neglected pathogens in vulnerable communities. Our findings provide a foundation for larger epidemiological studies that incorporate clinical data and support the development of cost-effective monitoring tools for FLA-associated public health threats in sub-Saharan Africa.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Electron Microscopy Reveals the Structural Complexity of the Intravacuolar Membranous Network in Cyrilia lignieresi-Infected Erythrocytes of the Fish Synbranchus marmoratus 三维电子显微镜揭示了木质素西霉感染的马氏合鳃鱼红细胞的孔内膜网络结构的复杂性

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-20 DOI: 10.1111/jeu.70031

Brenda Santarém Fachetii, Maíra Turiel-Silva, Camila Wendt, Hilton Tulio Costi, Edilene Oliveira da Silva, Ana Paula Drummond Rodrigues, Wanderley de Souza, Kildare Miranda, José Antonio Picanço Diniz

{"title":"3D Electron Microscopy Reveals the Structural Complexity of the Intravacuolar Membranous Network in Cyrilia lignieresi-Infected Erythrocytes of the Fish Synbranchus marmoratus","authors":"Brenda Santarém Fachetii, Maíra Turiel-Silva, Camila Wendt, Hilton Tulio Costi, Edilene Oliveira da Silva, Ana Paula Drummond Rodrigues, Wanderley de Souza, Kildare Miranda, José Antonio Picanço Diniz","doi":"10.1111/jeu.70031","DOIUrl":"https://doi.org/10.1111/jeu.70031","url":null,"abstract":"This study employs advanced three-dimensional electron microscopy techniques, including Transmission Electron Microscopy (TEM) tomography and freeze-fracture imaging via scanning electron microscopy (SEM), to investigate the ultrastructural organization of Cyrilia lignieresi-infected red blood cells (iRBCs) in the host fish Synbranchus marmoratus. The analysis focuses on the parasitophorous vacuole (PV) and reveals a highly complex intravacuolar membranous network (IVN) composed of vesicles, tubules, and interconnected membranous structures. These elements exhibit considerable diversity in size, morphology, and electron density, suggesting dynamic functional roles in the parasite–host interaction. The electron tomography and three-dimensional reconstructions data provide unprecedented insights into the spatial organization and potential functional significance of these membranous systems. These findings not only enhance our understanding of the cellular adaptations of C. lignieresi but also contribute to a broader knowledge of apicomplexan parasitism and host–pathogen interactions.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host Organelle Interactions Facilitate Cholesterol Acquisition by Trypanosoma cruzi Amastigotes 宿主细胞器相互作用促进克氏锥虫无轴虫获得胆固醇

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-20 DOI: 10.1111/jeu.70027

Carolina de Lima Alcantara, Miria Gomes Pereira, Wanderley de Souza, Narcisa Leal da Cunha-e-Silva

{"title":"Host Organelle Interactions Facilitate Cholesterol Acquisition by Trypanosoma cruzi Amastigotes","authors":"Carolina de Lima Alcantara, Miria Gomes Pereira, Wanderley de Souza, Narcisa Leal da Cunha-e-Silva","doi":"10.1111/jeu.70027","DOIUrl":"https://doi.org/10.1111/jeu.70027","url":null,"abstract":"Chagas disease, caused by the protozoan Trypanosoma cruzi, is a major neglected disease in Latin America. The amastigote, the replicative intracellular form, is essential for infection persistence in vertebrate hosts. These forms exhibit remarkable adaptability, modulating metabolism and growth according to host cell resource availability. Lipid metabolism plays a critical role in amastigote development, with a strong dependence on host-derived lipids, particularly cholesterol. Although T. cruzi can synthesize some sterols and fatty acids, it also scavenges essential lipids from the host. Changes in host cholesterol metabolism, possibly via SREBPs, may increase intracellular cholesterol levels and promote parasite growth. However, the mechanisms of cholesterol acquisition by amastigotes remain unclear. Here, we investigated cholesterol trafficking from host cells to amastigotes using a fluorescent cholesterol analog. Through confocal and volume electron microscopy, we demonstrated cholesterol uptake by amastigotes, characterized uptake kinetics, and confirmed its importance for parasite development. We also revealed close contact between the host endoplasmic reticulum and the amastigote plasma membrane, consistent with membrane contact sites. Furthermore, we showed that amastigotes can internalize ER- and Golgi-derived host markers, suggesting a potential route for acquisition of host molecules. These findings provide new insights into lipid acquisition strategies by intracellular T. cruzi amastigotes.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to Morphology, Systematics and Life Cycle of Ozanamia fimbriatus (Haptista: Centroplasthelida), With Notes on Evolution of Organic Skeleton in Centrohelids 毛蕊虫形态、分类学和生活史的修正——附关于中心体有机骨架演化的注解

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-16 DOI: 10.1111/jeu.70029

引用次数: 0