Journal of Eukaryotic Microbiology最新文献_第2页

The nuclear DNA and RNA distribution in Pelomyxa spp. (Amoebozoa, Archamoebae, Pelobiontida) revealed by a simple-to-use DAPI/pyronin staining method

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-01-27 DOI: 10.1111/jeu.70000

Dmitry S. Bogolyubov, Ludmila V. Chistyakova, Yuliya Y. Sokolova, Andrew V. Goodkov

引用次数: 0

Ultrastructure of Olkasia polycarbonata (Euglenozoa, Euglenida) demonstrates cytoskeletal innovations associated with the feeding and flagellar apparatuses

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-01-27 DOI: 10.1111/jeu.13074

Maia V. Palka, Regine Claire Manglicmot, Gordon Lax, Kevin C. Wakeman, Brian S. Leander

{"title":"Ultrastructure of Olkasia polycarbonata (Euglenozoa, Euglenida) demonstrates cytoskeletal innovations associated with the feeding and flagellar apparatuses","authors":"Maia V. Palka, Regine Claire Manglicmot, Gordon Lax, Kevin C. Wakeman, Brian S. Leander","doi":"10.1111/jeu.13074","DOIUrl":"10.1111/jeu.13074","url":null,"abstract":"Euglenids are flagellates with diverse modes of nutrition, including the photosynthetic Euglenophyceae, which acquired plastids via secondary endosymbiosis with green algae, and a diverse assemblage of predators of bacteria and other microeukaryotes. Most heterotrophic euglenids have never been cultivated, so their morphology remains poorly characterized and limited to only a few studies. “Ploeotids” are a paraphyletic group representing much of the diversity of heterotrophic euglenids and are characterized by their feeding apparatus and a rigid pellicle of 10–12 longitudinally arranged strips. Ploeotid-like euglenids gave rise to the Spirocuta, a large clade of heterotrophic and photosynthetic euglenids defined by a flexible pellicle of helically arranged strips. Using single-cell approaches, we report the first ultrastructural characterization of Olkasia polycarbonata, a ploeotid that is consistently positioned as the sister lineage to the Spirocuta in multigene phylogenetic analyses. O. polycarbonata shares several morphological characteristics with members of Spirocuta, such as prominent swellings on the paraxonemal rods and a robust feeding apparatus consisting of rods and vanes. These morphological traits are consistent with the phylogenetic position of O. polycarbonata and demonstrate an increase in cytoskeletal complexity that occurred prior to the key strip duplication event in the most recent common ancestor of Spirocuta.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to How to overcome constraints imposed by microsporidian genome features to ensure gene prediction?

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-01-27 DOI: 10.1111/jeu.13069

引用次数: 0

Phylogenetic and structural characterization of Kentomonas inusitatus n. sp.: Unique insect trypanosomatid of the Strigomonadinae subfamily naturally lacking bacterial endosymbiont 内生肯托单胞菌的系统发育和结构特征：天然缺乏细菌内共生体的单胞单胞菌亚科特有的昆虫锥虫。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-01-21 DOI: 10.1111/jeu.13083

Maria Cristina Machado Motta, Tayná Mourão Camelo, Camillo Martins Costa Cerdeira, Camila Silva Gonçalves, Tarcilla Corrente Borghesan, Evaristo Villalba-Alemán, Wanderley de Souza, Marta Maria Geraldes Teixeira, Erney Felicio Plessmann de Camargo

{"title":"Phylogenetic and structural characterization of Kentomonas inusitatus n. sp.: Unique insect trypanosomatid of the Strigomonadinae subfamily naturally lacking bacterial endosymbiont","authors":"Maria Cristina Machado Motta, Tayná Mourão Camelo, Camillo Martins Costa Cerdeira, Camila Silva Gonçalves, Tarcilla Corrente Borghesan, Evaristo Villalba-Alemán, Wanderley de Souza, Marta Maria Geraldes Teixeira, Erney Felicio Plessmann de Camargo","doi":"10.1111/jeu.13083","DOIUrl":"10.1111/jeu.13083","url":null,"abstract":"All insect trypanosomatids of the subfamily Strigomonadinae harbor a proteobacterial symbiont in their cytoplasm and unique ultrastructural cell organization. Here, we report an unexpected finding within the Strigomonadinae subfamily: the identification of a new species lacking bacterial symbiont, represented by two isolates obtained from Calliphoridae flies in Brazil and Uganda. This species is hereby designated as Kentomonas inusitatus n. sp. Molecular investigations targeting symbiont DNA, cell proliferation, and ultrastructural analyses agreed with the absence of bacterial symbionts in cultured flagellates. PCR-screening specifically targeting symbiont DNA corroborated the absence of symbionts in K. inusitatus present in the intestine of the respective host flies. K. inusitatus exhibited forms varying in size and shape. While displaying overall ultrastructural features of the Strigomonadinae, the novel species showed mitochondrial branches juxtaposed to the plasma membrane in locations both without and notable, with subpellicular microtubules. The discovery of the first Strigomonadinae species naturally lacking a symbiont and closely related to K. sorsogonicus, suggests a unique evolutionary history for the genus Kentomonas. Our findings provide novel insights into the complex relationships between trypanosomatids and their symbionts.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of amphisterol and other Δ8(14) sterols by Togula jolla (formerly Amphidinium sensu lato) overlaps chemotaxonomically with Amphidinium carterae 由Togula jolla（原Amphidinium sensu lato）生产的两栖甾醇和其他Δ8（14）甾醇在化学分类上与Amphidinium carterae重叠。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-01-19 DOI: 10.1111/jeu.13075

Braedyn E. Hollingsworth, Catharina Alves-de-Souza, Jeffrey D. Leblond

{"title":"Production of amphisterol and other Δ8(14) sterols by Togula jolla (formerly Amphidinium sensu lato) overlaps chemotaxonomically with Amphidinium carterae","authors":"Braedyn E. Hollingsworth, Catharina Alves-de-Souza, Jeffrey D. Leblond","doi":"10.1111/jeu.13075","DOIUrl":"10.1111/jeu.13075","url":null,"abstract":"While a longstanding method for identifying dinoflagellates, morphology alone can be inaccurate because of convergent evolution of truly different species toward a particular cellular shape. The dinoflagellate genus Togula is a case in point in that its type species Togula britannica was previously assigned to the genus Amphidinium based on morphology but was recently recognized as an independent genus following phylogenetic characterization. Chemotaxonomy can be a useful tool to complement the characterization of dinoflagellates by phylogeny. To this point, some, but not all, species of Amphidinium, such as Amphidinium carterae, have been observed to produce the rare 4α-methyl-substituted, Δ8(14)-nuclear-unsaturated major sterol 4α-methyl-5α-ergosta-8(14),24(28)-dien-3β-ol (amphisterol) that has historically been considered a potential chemotaxonomic biomarker for the genus as it is found in very few other dinoflagellate genera. To date, no isolates within the genus Togula have had their sterols characterized; our objective was thus to provide a first examination of the sterols of an isolate of Togula to compare to the sterols of Amphidinium. To this end, we have characterized the sterols of Togula jolla, one of the few members of Togula available for study, to demonstrate the production of amphisterol, among other Δ8(14)-nuclear unsaturated sterols, as its major sterol.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Katarium polorum n. sp., n. g., a novel thecofilosean amoeba (Cercozoa, Rhizaria) from the polar oceans polorum n. sp., n. g.，一种来自极地海洋的新型丝状阿米巴原虫（Cercozoa, Rhizaria）。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-11-29 DOI: 10.1111/jeu.13071

Marcel Dominik Solbach, Michael Bonkowski, Kenneth Dumack

引用次数: 0

Editorial Acknowledgment 编辑致谢。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-11-28 DOI: 10.1111/jeu.13068

引用次数: 0

Retention of blue-green cryptophyte organelles by Mesodinium rubrum and their effects on photophysiology and growth Mesodinium rubrum 对蓝绿隐花植物细胞器的保留及其对光生理学和生长的影响。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-11-25 DOI: 10.1111/jeu.13066

Holly V. Moeller, Amelie L'Etoile-Goga, Lucas Vincenzi, Andreas Norlin, Gina S. Barbaglia, Gabriel C. Runte, Jonatan T. Kaare-Rasmussen, Matthew D. Johnson

{"title":"Retention of blue-green cryptophyte organelles by Mesodinium rubrum and their effects on photophysiology and growth","authors":"Holly V. Moeller, Amelie L'Etoile-Goga, Lucas Vincenzi, Andreas Norlin, Gina S. Barbaglia, Gabriel C. Runte, Jonatan T. Kaare-Rasmussen, Matthew D. Johnson","doi":"10.1111/jeu.13066","DOIUrl":"10.1111/jeu.13066","url":null,"abstract":"As chloroplast-stealing or “kleptoplastidic” lineages become more reliant on stolen machinery, they also tend to become more specialized on the prey from which they acquire this machinery. For example, the ciliate Mesodinium rubrum obtains > 95% of its carbon from photosynthesis, and specializes on plastids from the Teleaulax clade of cryptophytes. However, M. rubrum is sometimes observed in nature containing plastids from other cryptophyte species. Here, we report on substantial ingestion of the blue-green cryptophyte Hemiselmis pacifica by M. rubrum, leading to organelle retention and transient increases in M. rubrum's growth rate. However, microscopy data suggest that H. pacifica organelles do not experience the same rearrangement and integration as Teleaulax amphioxeia's. We measured M. rubrum's functional response, quantified the magnitude and duration of growth benefits, and estimated kleptoplastid photosynthetic rates. Our results suggest that a lack of discrimination between H. pacifica and the preferred prey T. amphioxeia (perhaps due to similarities in cryptophyte size and swimming behavior) may result in H. pacifica ingestion Thus, while blue-green cryptophytes may represent a negligible prey source in natural environments, they may help M. rubrum survive when Teleaulax are unavailable. Furthermore, these results represent a useful tool for manipulating M. rubrum's cell biology and photophysiology.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.13066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine structural features of the free-living stages of Amyloodinium ocellatum (Dinoflagellata, Thoracosphaeraceae): A marine fish ectoparasite Amyloodinium ocellatum（甲壳纲，Thoracosphaeraceae）自由生活阶段的精细结构特征：一种海洋鱼类外寄生虫。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-11-18 DOI: 10.1111/jeu.13067

Zhicheng Li, Jingyu Zhuang, Jizhen Cao, Qing Han, Zhi Luo, Baotun Wang, Hebing Wang, Chuanfu Dong, Anxing Li

{"title":"Fine structural features of the free-living stages of Amyloodinium ocellatum (Dinoflagellata, Thoracosphaeraceae): A marine fish ectoparasite","authors":"Zhicheng Li, Jingyu Zhuang, Jizhen Cao, Qing Han, Zhi Luo, Baotun Wang, Hebing Wang, Chuanfu Dong, Anxing Li","doi":"10.1111/jeu.13067","DOIUrl":"10.1111/jeu.13067","url":null,"abstract":"Amyloodinium ocellatum is a protozoan parasite that causes amyloodiniosis in marine and brackish water fish, threatening global aquaculture. The present study investigates the morphology and ultrastructure of the free-living stages of A. ocellatum (tomont and dinospore) using light microscopy (LM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Dinospores measured 13.03–19.66 μm in length, 12.32–18.71 μm in width, and were laterally flattened. Dinospores had a transverse flagellum for propulsion and a longitudinal flagellum for direction control. The cyst wall had three distinct layers and included cellulose. The outer wall was coated with numerous bacteria. The orange-red speckled eyespot was observed all tomont developmental stages and in the dinospore of A. ocellatum. Tomonts proliferation required successive nuclear division, the formation of new cyst walls, and cytoplasmic segregation. The cytoplasm comprises mainly the matrix, organelles, and inclusions. The matrix was grainy and evenly distributed. In addition to organelles, including mitochondria with tubular cristae, Golgi apparatus, and endoplasmic reticulum, the cytoplasm had starch grains and lipid droplets as inclusions. The A. ocellatum cells lacked chloroplasts. This study provides the first ultrastructural view of the cytoplasmic structure of the free-living stages of A. ocellatum.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of protease inhibitors on the intraerythrocytic development of Babesia microti and Babesia duncani, the causative agents of human babesiosis 蛋白酶抑制剂对人类巴贝西亚原虫小巴贝西亚和邓卡尼巴贝西亚红细胞内发育的影响。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2024-11-18 DOI: 10.1111/jeu.13064

Temitope Aderanti, Jordan M. Marshall, Jose Thekkiniath

{"title":"Effect of protease inhibitors on the intraerythrocytic development of Babesia microti and Babesia duncani, the causative agents of human babesiosis","authors":"Temitope Aderanti, Jordan M. Marshall, Jose Thekkiniath","doi":"10.1111/jeu.13064","DOIUrl":"10.1111/jeu.13064","url":null,"abstract":"Human babesiosis is a malaria-like, tick-borne infectious disease with a global distribution. Babesiosis is caused by intraerythrocytic, apicomplexan parasites of the genus Babesia. In the United States, human babesiosis is caused by Babesia microti and Babesia duncani. Current treatment for babesiosis includes either the combination of atovaquone and azithromycin or the combination of clindamycin and quinine. However, the side effects of these agents and the resistance posed by these parasites call for alternative approaches for treating human babesiosis. Proteases play several roles in the context of parasitic lifestyle and regulate basic biological processes including cell death, cell progression, and cell migration. Using the SYBR Green-1 assay, we screened a protease inhibitor library that consisted of 160 compounds against B. duncani in vitro and identified 13 preliminary hits. Dose response assays of hit compounds against B. duncani and B. microti under in vitro conditions identified five effective inhibitors against parasite growth. Of these compounds, we chose ixazomib, a proteasome inhibitor as a potential drug for animal studies based on its lower IC50 and a higher therapeutic index in comparison with other compounds. Our results suggest that Babesia proteasome may be an important drug target and that developing this class of drugs may be important to combat human babesiosis.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0