Journal of Eukaryotic Microbiology最新文献_第3页

Experimental Evidence for Conservation of the Ubiquitin-Like Autophagy-Related ATG12 Conjugation System in Trichomonas vaginalis 阴道毛滴虫泛素样自噬相关ATG12偶联系统保存的实验证据

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-15 DOI: 10.1111/jeu.70028

Xiaoxia X. Lin, Yumiko Saito-Nakano, Euki Yazaki, Ryo Harada, Dingming V. Zhao, Kenji Hikosaka, Hirokazu Sakamoto

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Report of the 44th Annual Meeting of the Society of Eukaryotic Microbiology (EuMik) 第44届真核微生物学会年会报告

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-09 DOI: 10.1111/jeu.70024

Edward A. D. Mitchell

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How Single Cells Form Shells: Maturation and Secretion of Lorica-Forming Material in the Tintinnid Schmidingerella (Alveolata, Ciliophora) 单细胞是如何形成壳的：丁丁属Schmidingerella （Alveolata, Ciliophora）中lorica形成物质的成熟和分泌

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-08 DOI: 10.1111/jeu.70025

Maximilian H. Ganser, Birgit Weißenbacher, Sabine Agatha

{"title":"How Single Cells Form Shells: Maturation and Secretion of Lorica-Forming Material in the Tintinnid Schmidingerella (Alveolata, Ciliophora)","authors":"Maximilian H. Ganser, Birgit Weißenbacher, Sabine Agatha","doi":"10.1111/jeu.70025","DOIUrl":"https://doi.org/10.1111/jeu.70025","url":null,"abstract":"Tintinnid ciliates are distinguished by their loricae (shells), the key synapomorphy of these mainly marine planktonic protists. They can divide daily, with a considerable portion of biomass stored in the loricae. During each division, lorica-forming material (LFM) is produced and afterwards used by the proter (anterior division product) to construct a new lorica, while the opisthe (posterior division product) retains the parental one. Many aspects of lorica formation remain unclear, and no study describes the entire process from material maturation via secretion to assembly. Here, we present the first thorough investigation at cellular and sub-cellular levels, employing light microscopy on dividers and postdividers, as well as transmission electron microscopy on primarily cryofixed specimens from a Schmidingerella culture. Our study reconstructs LFM maturation, identifying two main developmental stages: the morula-shaped precursor granules and the mature granules. The latter cluster in the proter's ventral portion with a peripheral longitudinal strip of small granules embedded in large ones. Ultrastructurally and chemically, the mature granules of both size classes are identical. Through detailed live observations, we followed and documented, for the first time, the process of cell division, the behavior of the proter, the release of LFM granules, and features of lorica formation.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Large Genomes Are Associated With Greater Cell Size and Ecological Shift Towards More Nitrogen-Rich and Higher-Latitude Environments in Microalgae of the Genus Synura 大基因组与大细胞大小和向更富氮和高纬度环境的生态转移有关

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-07-02 DOI: 10.1111/jeu.70026

Dora Čertnerová, Pavel Škaloud, Iva Jadrná, Martin Čertner

{"title":"Large Genomes Are Associated With Greater Cell Size and Ecological Shift Towards More Nitrogen-Rich and Higher-Latitude Environments in Microalgae of the Genus Synura","authors":"Dora Čertnerová, Pavel Škaloud, Iva Jadrná, Martin Čertner","doi":"10.1111/jeu.70026","DOIUrl":"https://doi.org/10.1111/jeu.70026","url":null,"abstract":"The nuclear genome is essential for encoding most of the genes required for cellular processes, but its size alone can alter the characteristics of cells and organisms. Yet, genome size variation and its ecological and evolutionary impacts, particularly in microorganisms, are not well understood. We used flow cytometry to estimate genome size and GC content in 53 evolutionary lineages of the microalgal genus Synura (Chrysophyceae, Stramenopiles). Genome size evolution was reconstructed in a phylogenetic framework using molecular markers. A set of genomic, morphological, and ecogeographic variables characterizing Synura lineages was evaluated and tested as predictors of genome size variation in phylogeny-corrected statistical models. Both genome size and GC content varied widely in Synura, ranging from 0.19 to 3.70 pg of DNA and 34.0% to 49.3%, respectively. Genome size variation was mainly associated with cell size, less with silica scale size, and not with scale ultrastructure. Higher soil nitrogen, higher latitudes, and lower temperatures correlated with larger genomes. Genome size evolution in Synura shows potential dynamism, with increases confined to short terminal branches, indicating lower macroevolutionary stability. Lineages with larger genomes exhibited a narrower range of suitable ecological conditions, possibly due to selection acting deleteriously against larger genomes (and cells).","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Morphology, Systematics and Life Cycle of Ozanamia fimbriatus (Haptista: Centroplasthelida), With Notes on Evolution of Organic Skeleton in Centrohelids 毛蕊虫的形态、分类学和生活史（Haptista: centrroplasthelida）——兼论中心体有机骨架的进化

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-06-29 DOI: 10.1111/jeu.70022

Daria Drachko, Vasily V. Zlatogursky

{"title":"Morphology, Systematics and Life Cycle of Ozanamia fimbriatus (Haptista: Centroplasthelida), With Notes on Evolution of Organic Skeleton in Centrohelids","authors":"Daria Drachko, Vasily V. Zlatogursky","doi":"10.1111/jeu.70022","DOIUrl":"https://doi.org/10.1111/jeu.70022","url":null,"abstract":"<div>\u0000 \u0000 The majority of centrohelids bear coverings that consist either of siliceous scales or organic spicules. Strains carrying organic spicules appear in all major clades of scale-bearing centrohelids. Sometimes they represent alternative life cycle stages of scale-bearing species, whereas in other cases such strains do not alternate their morphology. Studying this “siliceous-organic” polymorphism is instrumental to understanding centrohelid diversity and evolution. The genus Chlamydaster has unique organic coverings that are formed with a mucous sheath and seemingly lack skeletal elements. Two centrohelid strains matching the description of Chlamydaster fimbriatus were isolated in clonal cultures and studied with the use of light and electron microscopy. The mucous sheath was shown to be covered with short, crisscrossed spicules. One of the strains formed cysts with siliceous spine scales, whereas the other did not encyst. SSU rDNA-based molecular phylogenetics placed the strains into the scale-bearing genus Ozanamia, distant from the type species of Chlamydaster. The new combination Ozanamia fimbriatus (Penard 1904) Drachko was established. The implications of Chlamydaster non-monophyly and the role of the “siliceous-organic” polymorphism in encystment were discussed.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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EXPRESSION OF CONCERN: Cystathionine γ-lyase, an Enzyme Related to the Reverse Transsulfuration Pathway, is Functional in Leishmania spp. 关注表达：半胱硫氨酸γ-裂解酶，一种与反转硫途径相关的酶，在利什曼原虫中有功能。

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-06-28 DOI: 10.1111/jeu.70021

{"title":"EXPRESSION OF CONCERN: Cystathionine γ-lyase, an Enzyme Related to the Reverse Transsulfuration Pathway, is Functional in Leishmania spp.","authors":"","doi":"10.1111/jeu.70021","DOIUrl":"https://doi.org/10.1111/jeu.70021","url":null,"abstract":"\u0000 EXPRESSION OF CONCERN: L. Giordana, B. S. Mantilla, M. Santana, A. M. Silber, and C. Nowicki, “ Cystathionine γ-lyase, an Enzyme Related to the Reverse Transsulfuration Pathway, is Functional in Leishmania spp.,” Journal of Eukaryotic Microbiology 61, no. 2 (2014): 204–213, https://doi.org/10.1111/jeu.12100.\u0000 This Expression of Concern is for the above article, published online on 11 January 2014 in Wiley Online Library (wileyonlinelibrary.com), and has been issued by agreement between the journal Editor-in-Chief, Joel B. Dacks; The International Society of Protistologists; and Wiley Periodicals LLC. The Expression of Concern has been agreed due to concerns raised by third parties. A duplication of the Western Blot bands depicting “CS_P” and “CGL_P” has been identified in Figure 5B. The authors have acknowledged an unintentional duplication of one of the western blot images corresponding to CS_P or CGL_P during figure preparation, rather than duplication of the individual band. The authors declare that none of the western blots in the figure were altered in any form. However, due to the time elapsed since publication, the original data could not be recovered to rectify the mistake. As the conclusions are not affected by the issue detected, the journal has decided to issue an Expression of Concern to inform and alert the readers.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Update on the Morphology and Phylogeny of the Nanoplanktonic Dinoflagellate Prorocentrum nux 纳米浮游鞭毛藻（Dinoflagellate proorocentrum nux）形态与系统发育研究进展

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-06-26 DOI: 10.1111/jeu.70019

Solenn Mordret, Jenna MacKinnon, Susana A. Breglia, Claudio H. Slamovits, Caroline Chénard

{"title":"An Update on the Morphology and Phylogeny of the Nanoplanktonic Dinoflagellate Prorocentrum nux","authors":"Solenn Mordret, Jenna MacKinnon, Susana A. Breglia, Claudio H. Slamovits, Caroline Chénard","doi":"10.1111/jeu.70019","DOIUrl":"https://doi.org/10.1111/jeu.70019","url":null,"abstract":"The Prorocentrales are a diverse group of dinophytes found in a wide range of marine environments. However, species delimitation in the genus Prorocentrum still remains a challenge, especially for nanoscale species where morphological and molecular information is often incomplete. Prorocentrum nux is an example where information is sparse and efforts are needed. In this study, we present a detailed description of the morphological features and molecular information for a Northwest Atlantic strain (AGSB-0131). Using Scanning Electron Microscopy, additional morphological features from the original description were highlighted, including the periflagellar area configuration (nine vs. seven platelets) and pattern dissimilarities for the large and marginal pores. In addition to the revised description, the 18S rRNA gene, partial 28S rRNA gene, mitochondrial cox1 and cob genes were generated from strain AGSB-0131, revealing some ambiguity in the existing data available for P. nux. An important genetic divergence between the only 18S rRNA sequence currently available for P. nux (strain RCC303) and AGSB-0131 was highlighted, suggesting the importance of using the same strain to retrieve morphological and molecular information for nanodinoflagellates. Adding genetic markers for the correct identification of P. nux will enhance the ability to access its prevalence in the marine environment.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970 Nematopsis Schneider， 1892年在圣基茨的Nerite腹足动物中发现，属的系统发育研究，并将其置于顶复合体门

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-06-24 DOI: 10.1111/jeu.70023

Nicole A. M. Herbert, Árni Kristmundsson, Nuria Vazquez, Kelsey Hoag, Mark A. Freeman

{"title":"Nematopsis Schneider, 1892 in Nerite Gastropods From Saint Kitts, With a Phylogenetic Study of the Genus, and Placement Within the Phylum Apicomplexa Levine, 1970","authors":"Nicole A. M. Herbert, Árni Kristmundsson, Nuria Vazquez, Kelsey Hoag, Mark A. Freeman","doi":"10.1111/jeu.70023","DOIUrl":"https://doi.org/10.1111/jeu.70023","url":null,"abstract":"The Apicomplexa are obligate unicellular parasites found in terrestrial and aquatic environments. Nematopsis are found infecting marine invertebrates in a life cycle involving mollusks and crustaceans. In the present study, mantle and muscle tissues from nerite gastropods were microscopically examined for Nematopsis using wet mounts and histology. Oocysts contained one single sporozoite surrounded by an ellipsoidal wall and were surrounded by a parasitophorous vacuole within a host phagocyte. Length and width of fresh oocysts were measured and compared between host species. DNA was extracted from infected tissue, and regions of the rRNA gene were amplified using novel primers. Nematopsis-infected tissues from scallops from Argentina and Scotland were used as controls. The sizes of oocysts observed in nerite hosts from Saint Kitts were not significantly different. DNA sequences of Nematopsis isolated from nerite hosts in this study were identical and phylogenetically related to sequences obtained from scallops. Bayesian and maximum likelihood phylogenetic analyses robustly place the Nematopsis DNA sequences from this study with Nematopsis from scallops in Florida and members of the family Porosporidae. We conclude that marine Nematopsis will group within this clade or within the Porosporidae. We have provided specific oligonucleotide primers to assist with the molecular study of the Porosporidae.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The Dinoflagellate Gonyaulax fragilis Is ‘the Wrong Man’ of the Dirty Seas, and Reinstatement of Steiniella Within the Protoceratiaceae (Dinophyceae) 脆弱的甲藻Gonyaulax fragilis是肮脏海洋的“错误的人”，以及原角虫科（甲藻科）Steiniella的恢复

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-06-11 DOI: 10.1111/jeu.70020

Fernando Gómez, Huan Zhang, Luis Felipe Artigas, Senjie Lin

{"title":"The Dinoflagellate Gonyaulax fragilis Is ‘the Wrong Man’ of the Dirty Seas, and Reinstatement of Steiniella Within the Protoceratiaceae (Dinophyceae)","authors":"Fernando Gómez, Huan Zhang, Luis Felipe Artigas, Senjie Lin","doi":"10.1111/jeu.70020","DOIUrl":"https://doi.org/10.1111/jeu.70020","url":null,"abstract":"<div>\u0000 \u0000 The production of mucilage aggregates is commonly associated with diatoms, but the harmful events known as ‘mare sporco’ or dirty seas have been attributed to the dinoflagellate Gonyaulax fragilis or G. hyalina. The synonymy of these taxa has been debated for decades. We report observations of G. fragilis from the Mediterranean Sea, and Atlantic and Indian Oceans, along with its SSU- and LSU rRNA gene and ITS region sequences from cells of the open Indian Ocean. The rDNA/ITS sequences are distantly related to those identified as G. fragilis from the Mediterranean Sea and New Zealand involved in dirty sea events. Unequivocally, G. fragilis is not the culprit of the mucilage events. Gonyaulax fragilis clusters with full support alongside with Schuettiella mitra, forming a sister group to the benthic genus Carinadinium, as an earlier branching lineage with the Protoceratiaceae (Ceratocorys, Pentaplacodinium, Protoceratium). A single anterior intercalary plate (1a) characterizes these genera, while the Gonyaulacaceae possess 2a. The genus Steiniella, first described for G. fragilis and S. mitra, has historically been synonymized with Gonyaulax. We reinstate Steiniella for these two species, and S. inflata, placing Schuettiella as a junior synonym. The species responsible of the mucilage events is attributed to G. hyalina.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Identity and Taxonomic Circumscription of the Pfiesteriacean Genera Aduncodinium and Speroidium (Dinophyceae) 拟拟藻科Aduncodinium属和Speroidium属的鉴定与分类界限

IF 2.1 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2025-06-07 DOI: 10.1111/jeu.70015

Albert Reñé, Aika Yamaguchi, Takeo Horiguchi, António José Calado, Mona Hoppenrath

{"title":"On the Identity and Taxonomic Circumscription of the Pfiesteriacean Genera Aduncodinium and Speroidium (Dinophyceae)","authors":"Albert Reñé, Aika Yamaguchi, Takeo Horiguchi, António José Calado, Mona Hoppenrath","doi":"10.1111/jeu.70015","DOIUrl":"https://doi.org/10.1111/jeu.70015","url":null,"abstract":"With its former circumscription, the genus Katodinium included purportedly naked species with a much larger epi- than hypocone, several of which were shown to be distantly related to the type species K. nieuportense. Most of these species have recently been transferred to other genera, several of them newly established. Among these new genera, Aduncodinium was proposed to encompass Katodinium glandula (=Gymnodinium glandula), and Speroidium was erected to encompass Katodinium fungiforme (=Gymnodinium fungiforme). However, the genus Aduncodinium was invalidly published and several morphological aspects described need to be re-evaluated. On the other hand, the inclusion in K. fungiforme of morphological and molecular characters observed in the strain known as VDH034S “Bullet,” a pfiesteriacean, needs re-evaluation because the latter shows a higher resemblance to Aduncodinium species than to K. fungiforme. The present contribution aimed to clarify the identity of the taxa involved on the basis of newly collected morphological and molecular information, and thereby avoid the nomenclatural and taxonomical uncertainties raised by previously published concepts of the genera Aduncodinium and Speroidium.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"72 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0