Journal of Eukaryotic Microbiology最新文献_第3页

Labyrinthula merlionensis sp. nov.: A Novel Labyrinthulid Infecting Marine Diatoms merlionensis sp. nov.：一种感染海洋硅藻的新型迷路虫。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-08-05 DOI: 10.1111/jeu.70111

Clarence Wei Hung Sim, Marie Walde, Hanna Strindberg, Avneet Kaur, Sophie Le Panse, Priscillia Gourvil, Jens Jahren, Daniel Vaulot, Adriana Lopes dos Santos

{"title":"Labyrinthula merlionensis sp. nov.: A Novel Labyrinthulid Infecting Marine Diatoms","authors":"Clarence Wei Hung Sim, Marie Walde, Hanna Strindberg, Avneet Kaur, Sophie Le Panse, Priscillia Gourvil, Jens Jahren, Daniel Vaulot, Adriana Lopes dos Santos","doi":"10.1111/jeu.70111","DOIUrl":"10.1111/jeu.70111","url":null,"abstract":"<div>\u0000 \u0000 Labyrinthulomycetes are a class of fungus-like heterotrophic protists from the Stramenopiles lineage, recognized for their ecological role as decomposers and contributors to nutrient cycling. They colonize various substrates, from seaweed to terrestrial environments, utilizing ectoplasmic networks for nutrient absorption. This study characterized a novel Labyrinthula strain associated with the marine diatom Biddulphia. Phylogenetic analysis of the full-length 18S rRNA gene positioned this strain as a new species, Labyrinthula merlionensis sp. nov. Scanning electron and light microscopy observations revealed bi-flagellated zoospores and spindle-shaped vegetative cells with ectoplasmic networks. Time-series observations of the interactions between L. merlionensis and Biddulphia were categorized into different phases: establishment, infection, and aggregation. Scanning electron and confocal microscopy observations during the infection phase established the use of ectoplasmic nets to target the marginal ridge regions between diatoms, and the detection of labyrinthulid cells within diatom frustules. These findings enhance the understanding of the diversity, morphology, and ecological roles of Labyrinthulomycetes, particularly their intra- and extra-cellular interactions with diatom hosts.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Riding the Storm: Microeukaryote Community Responses to Hurricane Disturbance 乘着风暴：微真核生物群落对飓风扰动的反应。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-08-05 DOI: 10.1111/jeu.70113

Andrea Aurora Rivera-Zizumbo, Patricia Velez, Yunuen Tapia-Torres, Luis E. Servín-Garcidueñas, Omar Lagunas-Calvo, Jaime Gasca-Pineda

{"title":"Riding the Storm: Microeukaryote Community Responses to Hurricane Disturbance","authors":"Andrea Aurora Rivera-Zizumbo, Patricia Velez, Yunuen Tapia-Torres, Luis E. Servín-Garcidueñas, Omar Lagunas-Calvo, Jaime Gasca-Pineda","doi":"10.1111/jeu.70113","DOIUrl":"10.1111/jeu.70113","url":null,"abstract":"Sandy beaches are dynamic ecosystems where terrestrial and marine organisms coexist, resulting in diverse communities where microeukaryotes (protists, microalgae, and meiofauna) are key drivers. Understanding their diversity patterns and responses to stressors is essential for elucidating ecological processes, yet information on this subject remains limited. Herein, microeukaryotic diversity in a sandy beach in Acapulco, Guerrero, was analyzed via ITS1 metabarcoding assessing community structure changes following hurricane Otis. Overall, the number of ASVs decreased substantially from 260 before the hurricane to 93 after. Groups like Porifera, Platyhelminthes, Rotifera, Amoeboza, and Choanoflagellata disappeared after the hurricane. Ochrophyta, Cnidaria, Ctenophora, and metazoans in general decreased in richness and abundance, whereas Ciliophora proliferated, particularly toward the supralittoral zone. ASVs turnover varied among zones; middle and lower coastal zones lost over 50% ASVs, and the supralittoral zone almost doubled its richness. These changes in community structure were related to total phosphorus and total nitrogen concentrations, suggesting possible bottom-up effects where nutrients available influence microbial resources for heterotrophic microeukaryotes. The observed patterns reflect high sensitivity of microeukaryotes to high-impact natural disturbances and suggest low recovery potential within the first 6 months following the event. Whether recovery occurs over longer interannual scales remains to be evaluated.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13439162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive Morphometric Plasticity in Gregarina polymorpha Infecting Tenebrio molitor Larvae 侵染黄粉虫幼虫的多形黄粉虫生殖形态的可塑性

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-07-31 DOI: 10.1111/jeu.70110

Alejandro Javier Correa-Espinosa, Gregorio Pérez-Cordón

{"title":"Reproductive Morphometric Plasticity in Gregarina polymorpha Infecting Tenebrio molitor Larvae","authors":"Alejandro Javier Correa-Espinosa, Gregorio Pérez-Cordón","doi":"10.1111/jeu.70110","DOIUrl":"https://doi.org/10.1111/jeu.70110","url":null,"abstract":"Morphological variability remains one of the principal challenges in the taxonomy and species delimitation of gregarines (Apicomplexa: Gregarinomorphea). In the present study, we investigated morphometric variability in gametocysts and syzygies of Gregarina polymorpha infecting Tenebrio molitor larvae through quantitative morphometric and molecular analyses. A total of 100 gametocysts and 100 independent syzygies were examined using light microscopy and digital morphometry. Gametocyst size exhibited extensive variability, with estimated areas ranging from 4850 to 30,844 μm2 and coefficients of variation exceeding 30%. Size distributions significantly departed from normality and displayed marked heterogeneity associated with the presence of unusually small gametocysts. However, Hartigan's dip test did not detect statistically significant deviation from unimodality. Within syzygies, primite and satellite areas were strongly positively correlated (Pearson's r = 0.726, p < 0.001), and standard major axis regression indicated growth patterns consistent with approximate isometry despite substantial dispersion among larger associations. Molecular characterization of small and large gametocyst pools recovered identical SSU rRNA sequences, indicating that the observed variability occurs within the same species. These results reveal substantial reproductive morphometric plasticity in G. polymorpha and highlight limitations of morphology alone for species delimitation in gregarines.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Genomes of Klossia Species: The First Complete Extrachromosomal Sequence Data From Parasites in the Monoxenous Apicomplexan Family Adeleidae Mesnil, 1903 (Apicomplexa: Adeleorina) 一种Klossia的线粒体基因组：首个来自单源apiccomplexan Family Adeleidae Mesnil寄生虫的完整染色体外序列数据，1903 （apiccomplexa: Adeleorina）。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-07-12 DOI: 10.1111/jeu.70107

Elizabeth G. Zeldenrust, Alexandre N. Léveillé, John R. Barta

{"title":"Mitochondrial Genomes of Klossia Species: The First Complete Extrachromosomal Sequence Data From Parasites in the Monoxenous Apicomplexan Family Adeleidae Mesnil, 1903 (Apicomplexa: Adeleorina)","authors":"Elizabeth G. Zeldenrust, Alexandre N. Léveillé, John R. Barta","doi":"10.1111/jeu.70107","DOIUrl":"10.1111/jeu.70107","url":null,"abstract":"Complete mitochondrial genomes were sequenced and annotated from three Klossia species (Apicomplexa; Adeleorina) infecting snails in North America and Europe. Mitochondrial genomes of K. helicina (type species—NC_058857.1; 6728 bp), K. razorbacki (NC_058856.1; 6775 bp), and K. vaderbriani (OM891106.1; 6757 bp) were circular-mapping with the same gene contents and organization with 3 CDS regions (cytochrome c subunits I [COI] and III [COIII], and cytochrome b [CytB]) interspersed with 37 large and small ribosomal DNA fragments. Content and organization of mitochondrial genomes of Klossia species were shared with Legerella nova (monoxenous adeleorinid protist infecting pill millipedes) except for the reversed coding direction of one rDNA fragment; Haemogregarina species (heteroxenous adeleorinid protists with leech definitive hosts) mitochondrial genomes shared similar genome organization but lacked 2 rDNA fragments found in those monoxenous genera. In contrast, mitochondrial genomes of the Klossia species differed markedly in organization from Klossiella equi, a monoxenous adeleorinid protist parasite of vertebrates. Pairwise comparisons of the CDS regions of the Klossia species to other adeleorinid protists confirmed the close relatedness of monoxenous L. nova (Legerellidae) to monoxenous Klossia species (Adeleidae). The diversity of mitochondrial genomes of monoxenous adeleorinid protists infecting invertebrates has only begun to be explored.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 4","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148430392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyrumthecina n. Infraord.: Revisiting the Morphological Diversity and Identifying the Phylogenetic Home of Argynnia Within Arcellinida (Tubulinea: Amoebozoa) Pyrumthecina（中国）；：阿米巴原虫（管目：阿米巴原虫）中银虫的形态多样性和系统发育家的确定。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-07-09 DOI: 10.1111/jeu.70109

Alfredo L. Porfirio-Sousa, Maria Beatriz Gomes E. Souza, Robert E. Jones, Matthew W. Brown, Daniel J. G. Lahr, Alexander K. Tice

{"title":"Pyrumthecina n. Infraord.: Revisiting the Morphological Diversity and Identifying the Phylogenetic Home of Argynnia Within Arcellinida (Tubulinea: Amoebozoa)","authors":"Alfredo L. Porfirio-Sousa, Maria Beatriz Gomes E. Souza, Robert E. Jones, Matthew W. Brown, Daniel J. G. Lahr, Alexander K. Tice","doi":"10.1111/jeu.70109","DOIUrl":"10.1111/jeu.70109","url":null,"abstract":"Arcellinida is a diverse lineage of testate amoebae within Amoebozoa, whose evolutionary history has been clarified through phylogenomics, resulting in a stable classification into suborders and infraorders. Yet, some taxa, such as the genus Argynnia, remain unplaced due to ambiguous morphology and uncertain positions in single-gene phylogenies. In this study, we explore Argynnia by presenting new records of species sampled across Brazil and address its longstanding taxonomic uncertainty through phylogenomics including the Protist 10,000 Genomes isolate P10K-MW-000941. Previous single-gene analyzes suggested a close phylogenetic affinity between this isolate and Argynnia. Here, we confirm this relationship and using a curated, contaminant-free 227-gene dataset generated through the PhyloFisher workflow, we reconstructed the phylogeny of Arcellinida and confirmed the placement of P10K-MW-000941. Our results recover this isolate as a well-supported long-branch, sister to the infraorder Longithecina. Considering this phylogenomic placement, the distinct morphology of Argynnia, and its consistent placement outside existing infraorders based on single-marker analyzes, we propose Pyrumthecina n. infraord. and Argynniidae n. fam. to accommodate P10K-MW-000941 and all Argynnia species. The recognition of Pyrumthecina refines our understanding of shell evolution and diversification within Arcellinida, while also underscoring the presence of additional deeply branching lineages among taxa currently classified as incertae sedis.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 4","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Atypical Aquaporin 1 Derived From Paramecium multimicronucleatum Functions as a Multifunctional Channel That Permeates Water, Glycerol, and Urea 来自多微核草履虫的非典型水通道蛋白1作为一个多功能通道，渗透水、甘油和尿素。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-07-03 DOI: 10.1111/jeu.70108

Masaki Ishida, Shuichi Ueno, Takashi Tominaga, Kurumi Tanaka, Akito Horikawa, Manabu Hori

{"title":"An Atypical Aquaporin 1 Derived From Paramecium multimicronucleatum Functions as a Multifunctional Channel That Permeates Water, Glycerol, and Urea","authors":"Masaki Ishida, Shuichi Ueno, Takashi Tominaga, Kurumi Tanaka, Akito Horikawa, Manabu Hori","doi":"10.1111/jeu.70108","DOIUrl":"10.1111/jeu.70108","url":null,"abstract":"Although it had been reported that the translation product of the functional aquaporin gene, aqp1, is localized to the contractile vacuole complex of Paramecium multimicronucleatum, the molecules that pass through this channel protein had not been identified. In the present study, we introduced and expressed this protein in Xenopus oocytes and identified the molecules that pass through this channel protein using a swelling assay. The aqp1 mRNA injected into Xenopus oocytes was found to be sufficiently expressed and functional within the oocytes. As a result, P. multimicronucleatum AQP1 (PmAQP1) allowed water to pass through at a relatively slow rate of approximately 50 × 10−4cm/s, a rate comparable to that of aquaporins present in the contractile vacuoles of other protists. This water permeability was almost completely inhibited by tetraethylammonium, an orthodox aquaporin inhibitor, and phloretin, an aquaglyceroporin inhibitor. PmAQP1 also had the ability to pass glycerol or urea through, but the rates of these processes could not be accurately measured in this study. These findings demonstrate that PmAQP1 is an atypical multifunctional channel, suggesting that the contractile vacuole of free-living protists plays a crucial role not only in osmoregulation but also in the clearance of metabolic wastes.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 4","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and Molecular Phylogeny of the Planktonic Dinoflagellate Mesoporos perforatus: A New Lineage of Prorocentrum (Prorocentrales, Dinophyceae) 浮游甲藻Mesoporos perforatus的形态与分子系统发育：甲藻科甲藻原系（proocentrales, Dinophyceae）

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-06-30 DOI: 10.1111/jeu.70105

Fernando Gómez, Alexander Francoeur, Huan Zhang, Jackson Sanders, Yifan Gu, Trenton Norred, Senjie Lin

{"title":"Morphology and Molecular Phylogeny of the Planktonic Dinoflagellate Mesoporos perforatus: A New Lineage of Prorocentrum (Prorocentrales, Dinophyceae)","authors":"Fernando Gómez, Alexander Francoeur, Huan Zhang, Jackson Sanders, Yifan Gu, Trenton Norred, Senjie Lin","doi":"10.1111/jeu.70105","DOIUrl":"10.1111/jeu.70105","url":null,"abstract":"<div>\u0000 \u0000 The dinoflagellate order Prorocentrales comprises two genera: Prorocentrum, which has been extensively studied, and the little-known Mesoporos. We established a monoclonal culture of Mesoporos perforatus from Long Island Sound, USA. The high variability in cell size (12–27 μm) and outline, together with irregular chloroplast morphology, suggests that previously described congeneric species may represent synonyms. The principal generic diagnostic character, commonly referred to as a pore, is not a true perforation but a conical depression located at the center of the theca; its function remains unknown. Cell morphology (cell size and shape, thecal ornamentation, and short apical spine) suggests affinities with P. cordatum and related species. However, a first detailed study of the periflagellar structure reveals differences such as platelet 8 separating platelets 2 and 3, a feature unreported in Prorocentrum species. Our first report of molecular data (SSU, LSU rDNA, and ITS regions) places M. perforatus as a species nested within Prorocentrum sensu stricto, with P. nux as the closest relative. Accordingly, we consider Mesoporos a synonym of Prorocentrum, and this species should be referred to as Prorocentrum perforatum (Gran) Krachmalny 1993, despite the absence of a true thecal perforation.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 4","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148352205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage-Specific Expression and Perinuclear Enrichment of γ-Tubulin During Tomont Development and Theront Morphogenesis in Cryptocaryon irritans 隐核虫包囊发育和形态发生过程中γ-微管蛋白的分期特异性表达和核周富集。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-06-29 DOI: 10.1111/jeu.70106

Dongqi Li, Yue Sun, Bowen Hou, Lei Shi, Fei Yin

{"title":"Stage-Specific Expression and Perinuclear Enrichment of γ-Tubulin During Tomont Development and Theront Morphogenesis in Cryptocaryon irritans","authors":"Dongqi Li, Yue Sun, Bowen Hou, Lei Shi, Fei Yin","doi":"10.1111/jeu.70106","DOIUrl":"10.1111/jeu.70106","url":null,"abstract":"<div>\u0000 \u0000 Cryptocaryon irritans is a lethal parasitic ciliate of marine fishes whose tomont stage is marked by repeated nuclear division and the morphogenesis of infective theronts, both dependent on dynamic microtubule reorganization. To investigate cytoskeleton-associated regulation during this proliferative process, we cloned and characterized the γ-tubulin gene (Ci-TUBG) and γ-tubulin complex protein 3 gene (Ci-TUBGCP3) from C. irritans. Sequence analysis showed that Ci-TUBG contains a canonical dual tubulin domain architecture and is highly conserved relative to homologs from Ichthyophthirius multifiliis and Tetrahymena thermophila. Structural modeling and molecular docking suggested a stable putative interaction interface between Ci-TUBG and Ci-TUBGCP3. Spatiotemporal expression analysis and immunofluorescence revealed stage-dependent upregulation and pronounced perinuclear enrichment of Ci-TUBG during tomont division, with persistent perinuclear localization in theronts. RNA interference showed that Ci-TUBG knockdown markedly reduced tomont hatching rate, decreased theront yield, and caused defects in theront morphogenesis accompanied by abnormal nuclear development. These findings indicate that Ci-TUBG is essential for tomont division and theront morphogenesis in C. irritans and provide a basis for understanding cytoskeleton-associated developmental regulation in this parasitic ciliate.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 4","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Contribution to the Morphologic Variable Morphs and Diversity in Microthoracida 对胸小体形态变异和多样性的贡献。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-06-17 DOI: 10.1111/jeu.70104

Fan Wu, Xuetong Zhao, Yaohan Jiang, Xiangrui Chen

{"title":"A Contribution to the Morphologic Variable Morphs and Diversity in Microthoracida","authors":"Fan Wu, Xuetong Zhao, Yaohan Jiang, Xiangrui Chen","doi":"10.1111/jeu.70104","DOIUrl":"10.1111/jeu.70104","url":null,"abstract":"<div>\u0000 \u0000 The nassophorean ciliates are a key group for understanding morphological diversification, but their phylogenetic relationships remain elusive. Using morphological characteristics, molecular data, and phylogenetic analyses, we attempt to cast light on species relationships within the order Microthoracida Jankowski, 1967. The present multifaceted approach reveals that (1) Leptopharynx costatus costatus Mermod, 1914 exhibits polymorphism, occurring in a large morph (35–55 × 25–40 μm) with a narrow oral basket and an intermediate mean of 228 basal bodies, supporting basal body count as a key diagnostic character; (2) comprehensive morphological and corresponding molecular information for Pseudomicrothorax dubius Maupas, 1883 was provided for the first time; (3) comparisons among different populations and morphs of L. costatus costatus and P. dubius show that the former is more morphologically variable, while the latter exhibits higher molecular variability; (4) SSU rDNA sequences provided in this work cluster with the sequences of conspecific populations, supporting the conclusion that both the families Microthoracidae Wrześniowski, 1870 (containing Leptopharynx Mermod, 1914) and the family Pseudomicrothoracidae Jankowski, 1967 (containing Pseudomicrothorax Mermod, 1914) are monophyletic.\u0000 </div>","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 4","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148271602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphomolecular Characterization of Two New Species of the Genus Bothrostoma Stokes, 1887 (Ciliophora: Metopidae) 两新种的形态分子特征（纤毛虫目：甲螨科）。

IF 3.3 4区生物学

Journal of Eukaryotic Microbiology Pub Date : 2026-06-15 DOI: 10.1111/jeu.70103

Quoc Dung Nguyen, Nanda Dwi Kristanti, Borong Lu, Novia Cahyani, Mann Kyoon Shin

{"title":"Morphomolecular Characterization of Two New Species of the Genus Bothrostoma Stokes, 1887 (Ciliophora: Metopidae)","authors":"Quoc Dung Nguyen, Nanda Dwi Kristanti, Borong Lu, Novia Cahyani, Mann Kyoon Shin","doi":"10.1111/jeu.70103","DOIUrl":"10.1111/jeu.70103","url":null,"abstract":"Although species diversity has been extensively studied in anaerobic ciliates, their full diversity is incompletely understood. More research is required to gain further insights into their diversity. In this study, two new metopid ciliates, namely, Bothrostoma oryzaforme nov. sp. and Bothrostoma turgidoryzaforme nov. sp., isolated from the sediments of freshwater ponds in South Korea, were investigated using live observations and protargol impregnation. Bothrostoma oryzaforme nov. sp. is characterized by an elongated ellipsoidal shape, an anterior end with a rounded snout, and a posterior end that is either rounded or truncated. Cortical granules are arranged in 4–5 short, oblique rows; perizonal stripe even-type. Bothrostoma turgidoryzaforme nov. sp. is distinguished from its congeners by its small body size, body shape broadly ellipsoidal to oval with a rounded snout, and posterior end rounded. Cortical granules are arranged in 5–7 short, oblique rows; perizonal stripe 4 + 1 type. SSU rDNA sequence-based phylogeny revealed the evolutionary positions of the two new species, further supporting their novelty.","PeriodicalId":15672,"journal":{"name":"Journal of Eukaryotic Microbiology","volume":"73 4","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jeu.70103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148258270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0