Ultrastructural and molecular characterization of Glugea sp. (microsporidia), a parasite of the Red Sea fish Carangoides bajad (Carangidae)

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Abdel‐Azeem S. Abdel‐Baki, Shawky M. Aboelhadid, Heba Abdel‐Tawab, Sónia Rocha, Manal Ahmed, Saleh Al‐Quraishy, Lamjed Mansour
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Abstract

Glugea sp. found infecting the liver of the teleost fish Carangoides bajad from the Red Sea, Egypt, is described based on light microscopy and ultrastructural characteristics combined with phylogenetic analyses. This microsporidium forms whitish xenomas up to ~4 mm in size. Xenomas display numerous parasitophorous vacuoles totally filled by mature spores, no other life cycle stages were observed. Mature spores ellipsoidal and measuring 6.3 × 4.0 μm in size. The polaroplast appears composed of two distinct regions: an electron‐dense vesicular region and a densely packed lamellar region. The polar tubule forms approximately 24–27 coils arranged in three layers encircling the posterior vacuole. The small subunit (SSU) rRNA gene and its ITS region were sequenced and showed the highest similarity of 99.4% to other Glugea spp. Bayesian inference and maximum likelihood analyses place the novel isolate within the Glugea clade, more specifically within a subclade that predominantly grouped species described from fish inhabiting the Arabian Gulf or Red Sea. The results validate the parasite's classification in the Glugea genus. Nevertheless, until more detailed ultrastructural and molecular data are obtained, the identification of the current Glugea species is hampered by the absence of some developmental stages and the high degree of genetic similarity.
红海鱼类 Carangoides bajad(鲤科)的寄生虫 Glugea sp.(微孢子虫)的超微结构和分子特征描述
根据光学显微镜和超微结构特征以及系统发育分析,描述了在埃及红海发现的感染远洋鱼类 Carangoides bajad 的肝脏的 Glugea sp.。这种微孢子虫形成的白色异瘤大小可达 ~4 毫米。异瘤上有许多寄生泡,完全由成熟孢子充满,没有观察到其他生命周期阶段。成熟孢子呈椭圆形,大小为 6.3 × 4.0 μm。极体似乎由两个不同的区域组成:电子致密的泡状区和致密的片状区。极管形成约 24-27 个线圈,分三层环绕后液泡。贝叶斯推断和最大似然法分析将该新分离物归入 Glugea 支系,更具体地说是归入一个亚支系,该亚支系主要归入居住在阿拉伯湾或红海的鱼类中的物种。这些结果验证了寄生虫在 Glugea 属中的分类。然而,在获得更详细的超微结构和分子数据之前,由于某些发育阶段的缺失和高度的遗传相似性,目前对 Glugea 物种的鉴定受到了阻碍。
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来源期刊
CiteScore
4.30
自引率
4.50%
发文量
85
审稿时长
6-12 weeks
期刊介绍: The Journal of Eukaryotic Microbiology publishes original research on protists, including lower algae and fungi. Articles are published covering all aspects of these organisms, including their behavior, biochemistry, cell biology, chemotherapy, development, ecology, evolution, genetics, molecular biology, morphogenetics, parasitology, systematics, and ultrastructure.
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