南太平洋陨石坑湖中的 Diphyllatea（CRuMs）转录组学证实了新的隐秘支系。

IF 2.1 4区生物学 Q3 MICROBIOLOGY

Journal of Eukaryotic Microbiology Pub Date : 2024-09-28 DOI:10.1111/jeu.13060

Luis Javier Galindo, Varsha Mathur, Hadleigh Frost, Guifré Torruella, Thomas A Richards, Nicholas A T Irwin

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引用次数: 0

摘要

Diphyllatea（CRuMs）是一种异养原生动物，目前分为三个不同的支系（Diphy I-III）。Diphy I 是 Diphylleia 属中的双鞭毛虫，而 Diphy II 和 III 则代表了由 Collodictyon 型四鞭毛虫组成的隐秘支系，这些支系最近根据 rRNA 基因系统进化被区分开来。在这里，我们从两个南太平洋岛屿的淡水火山口湖中分离出了 Diphyllatea，并从代表每个支系的物种中生成了高质量的转录组，包括来自 Diphy III 的首个转录组数据。系统发生组分析支持了 Diphy II 和 Diphy III 的分离，而转录组的完整性则凸显了这些数据在未来研究中的实用性。最后，我们讨论了这些偏远岛屿上 Diphyllatea 的生物地理学和生态学。

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Transcriptomics of Diphyllatea (CRuMs) from South Pacific crater lakes confirm new cryptic clades.

The Diphyllatea (CRuMs) are heterotrophic protists currently divided into three distinct clades (Diphy I-III). Diphy I are biflagellates in the genus Diphylleia, whereas Diphy II and III represent cryptic clades comprising Collodictyon-type quadriflagellates that were recently distinguished based on rRNA gene phylogenies. Here, we isolated Diphyllatea from freshwater crater lakes on two South Pacific islands and generated high-quality transcriptomes from species representing each clade, including the first transcriptomic data from Diphy III. Phylogenomic analyses support the separation of Diphy II and III, while transcriptome completeness highlights the utility of these data for future studies. Lastly, we discuss the biogeography and ecology of Diphyllatea on these remote islands.

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来源期刊

Journal of Eukaryotic Microbiology 生物-微生物学

CiteScore

4.30

自引率

4.50%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Eukaryotic Microbiology publishes original research on protists, including lower algae and fungi. Articles are published covering all aspects of these organisms, including their behavior, biochemistry, cell biology, chemotherapy, development, ecology, evolution, genetics, molecular biology, morphogenetics, parasitology, systematics, and ultrastructure.