Refurbishing the marine parasitoid order Pirsoniales with newly (re)described marine and freshwater free-living predators.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Kristina I Prokina, Naoji Yubuki, Denis V Tikhonenkov, Maria Christina Ciobanu, Purificación López-García, David Moreira
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引用次数: 0

Abstract

Pirsoniales is a stramenopile order composed of marine parasitoids of diatoms with unique life cycle. Until recently, a single genus, Pirsonia, uniting six species, was known. The recent identification of new free-living eukaryotrophic Pirsoniales Pirsonia chemainus, Feodosia pseudopoda, and Koktebelia satura changed our understanding of this group as exclusively parasitic. However, their cell ultrastructure and feeding preferences were not fully studied due to the death of the cultures. In this study, we re-isolated some of these Pirsoniales and established six new strains exhibiting predatory behavior, including a first freshwater representative. This allowed us to describe five new genera and species, as well as to emend the diagnosis of the order Pirsoniales. The 18S rRNA gene phylogenetic analysis revealed the position of new strains within Pirsoniales and their relationships with parasitoid relatives and environmental sequence lineages. Feeding experiments on novel Pirsoniales strains using diverse algal prey showed that they were not able to form trophosomes and auxosomes. The ability of cell aggregation in Pirsoniales was observed for the first time. One of the studied strains contained intracellular gammaproteobacteria distantly related to Coxiella. Ultrastructural analyses revealed a more complex cytoskeleton structure in Pirsoniales than previously thought and supported the monophyly of Bigyromonadea and Pseudofungi.

利用新近(重新)描述的海洋和淡水自由生活捕食者,重建海洋寄生虫目(Pirsoniales)。
硅藻寄生虫目(Pirsoniales)是由硅藻的海洋寄生虫组成的担子菌纲,具有独特的生命周期。直到最近,人们还只知道一个属--Pirsonia,共有 6 个种。最近发现了新的自由生活的真核营养型 Pirsoniales Pirsonia chemainus、Feodosia pseudopoda 和 Koktebelia satura,改变了我们对该类群只寄生的认识。然而,由于培养物死亡,我们未能对它们的细胞超微结构和摄食偏好进行全面研究。在这项研究中,我们重新分离了其中一些 Pirsoniales,并建立了六个表现出捕食行为的新菌株,包括第一个淡水代表菌株。这使我们得以描述五个新属和新种,并对 Pirsoniales 目进行了修正。18S rRNA 基因系统进化分析揭示了新菌株在 Pirsoniales 中的位置及其与寄生虫亲缘种和环境序列系的关系。利用不同的藻类猎物对新型 Pirsoniales 菌株进行的取食实验表明,它们不能形成滋养体和辅助体。首次观察到 Pirsoniales 的细胞聚集能力。所研究的菌株之一含有与柯西氏菌关系密切的胞内伽马蛋白菌。超微结构分析表明,Pirsoniales 的细胞骨架结构比以前认为的更为复杂,并支持 Bigyromonadea 和 Pseudofungi 的单系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.30
自引率
4.50%
发文量
85
审稿时长
6-12 weeks
期刊介绍: The Journal of Eukaryotic Microbiology publishes original research on protists, including lower algae and fungi. Articles are published covering all aspects of these organisms, including their behavior, biochemistry, cell biology, chemotherapy, development, ecology, evolution, genetics, molecular biology, morphogenetics, parasitology, systematics, and ultrastructure.
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