Ayşegül Beşir Özgeçen, Mustafa Mortaş, Fehmi Yazıcı
{"title":"Comparison of drying methods for instant colostrum powder production: fluid bed dryer as post-drying approach.","authors":"Ayşegül Beşir Özgeçen, Mustafa Mortaş, Fehmi Yazıcı","doi":"10.1017/S0022029926102684","DOIUrl":"https://doi.org/10.1017/S0022029926102684","url":null,"abstract":"<p><p>Colostrum is a valuable functional ingredient thanks to its rich composition of bioactive compounds, particularly immunoglobulins and growth factors, as well as its nutritional value. In addition to its use for calf nutrition and immune system support, colostrum is used to produce functional foods for human consumption. Various methods are available to prevent colostrum waste and to process it into various products. Traditional drying methods, such as spray and freeze drying, are widely used to extend shelf life. However, the resulting powders often exhibit poor instant drying properties, such as low dispersibility, solubility and wettability. Fluidized bed drying has emerged as a promising post-drying technology to address these limitations. This technique enables particle agglomeration and surface modification, increasing powder flowability, reducing cohesiveness and improving reconstitution behaviour in aqueous media. Furthermore, binders such as maltodextrin, lactose or sucrose during fluidized bed agglomeration can further stabilize the immunoglobulin content and reduce moisture uptake during storage. Optimizing process parameters such as inlet air temperature, binder concentration and fluidization air flow can yield colostrum powders with superior technological and functional properties. In this review, drying methods of colostrum powder are discussed comparatively and the potential of using the agglomeration process with a fluidized bed dryer to improve powder properties after drying is discussed. Consequently, fluidized bed drying represents a valuable strategy for developing instant colostrum powders, facilitating their application in various functional food matrices and increasing consumer acceptance.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-13"},"PeriodicalIF":1.6,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Protease specificity governs antioxidant peptide release and activity from camel milk β-casein.","authors":"Dalila Almi, Hillal Sebbane, Lamia Bouadjela, Saliha Si Ahmed, Chahra Senoussi, Karim Houali","doi":"10.1017/S0022029926102672","DOIUrl":"https://doi.org/10.1017/S0022029926102672","url":null,"abstract":"<p><p>Although camel milk β-casein is a promising source of bioactive peptides, the impact of protease specificity on antioxidant peptide release remains poorly understood. This study investigated how enzyme type shapes the hydrolysis profile and antioxidant capacity of β-casein in camel milk. Purified β-casein was obtained by anion-exchange chromatography and hydrolysed <i>in vitro</i> with pepsin, trypsin or chymotrypsin for 4 h. The degree of hydrolysis was quantified by the trinitrobenzenesulphonic acid assay, and structural changes and peptide patterns were characterised by urea-polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulphate-PAGE (SDS-PAGE) and reversed-phase high-performance liquid chromatography. Antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging and ferric reducing antioxidant power (FRAP) assays. Protease specificity significantly influenced hydrolysis kinetics and peptide composition (<i>P</i> < 0.05). Pepsin yielded the highest degree of hydrolysis and the most diverse peptide profile, which correlated with superior antioxidant activity. All hydrolysates exhibited significantly enhanced radical scavenging and reducing power compared to intact β-casein (<i>P</i> < 0.05). Pepsin-derived peptides showed the lowest half-maximal inhibitory concentrations (IC50) values in DPPH (0.51 mg/ml) and ABTS (0.64 mg/ml) assays and the highest FRAP values, outperforming chymotrypsin and trypsin hydrolysates. ABTS•<sup>+</sup> scavenging was consistently higher than DPPH• scavenging across all samples. These findings demonstrate that enzyme selection critically governs antioxidant peptide formation from camel β-casein via differential cleavage patterns. Pepsin hydrolysis represents an effective approach for generating potent antioxidant peptides from camel β-casein with potential application in dairy-based functional foods.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148976950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Carolina Narval de Araújo, Milene Lopes Dos Santos, Uriel Secco Londero, Antonio Amaral Barbosa, Francisco Augusto Burkert Del Pino, Viviane Rohrig Rabassa, Osvaldo Sousa, Nathaly Ana Carpinelli, Eliza Rossi Komninou, Rodrigo de Almeida, Marcio Nunes Corrêa
{"title":"Effect of bovine appeasing substance on multiparous Holstein cows during the transition period- CORRIGENDUM.","authors":"Maria Carolina Narval de Araújo, Milene Lopes Dos Santos, Uriel Secco Londero, Antonio Amaral Barbosa, Francisco Augusto Burkert Del Pino, Viviane Rohrig Rabassa, Osvaldo Sousa, Nathaly Ana Carpinelli, Eliza Rossi Komninou, Rodrigo de Almeida, Marcio Nunes Corrêa","doi":"10.1017/S0022029926102313","DOIUrl":"https://doi.org/10.1017/S0022029926102313","url":null,"abstract":"","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1"},"PeriodicalIF":1.6,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Whole-genome safety assessment of <i>Loigolactobacillus coryniformis</i> WBB05 and identification of a candidate gene for aerobic reuterin production.","authors":"Harutoshi Tsuda","doi":"10.1017/S0022029926102659","DOIUrl":"https://doi.org/10.1017/S0022029926102659","url":null,"abstract":"<p><p>This study reports on the safety profile of <i>Loigolactobacillus coryniformis</i> WBB05 for food industry applications and identifies glycerol-3-phosphate oxidase (<i>GlpO</i>) as a candidate gene associated with aerobic reuterin production. The safety of <i>L. coryniformis</i> WBB05 was evaluated through whole-genome sequencing, phenotypic analysis of haemolytic activity and determination of minimum inhibitory concentrations (MICs) of antibiotics. Comparative genomic analysis was performed to identify candidate genetic determinants for aerobic reuterin production. The draft genome (2.83 Mb, 179 contigs) harboured no known virulence factors, acquired antimicrobial resistance (AMR) genes or biogenic amine biosynthetic genes. Prophage analysis identified only one incomplete prophage region, and four CRISPR-Cas systems (212 spacers) were consistent with phage defence capacity. Secondary metabolite analysis revealed biosynthetic gene clusters encoding a coagulin-like bacteriocin. No β-haemolytic activity was observed. The MICs of all antibiotics tested were below the European Food Safety Authority cut-off values except for kanamycin (128 mg/L), although no acquired AMR genes were detected. Comparative genomic analysis revealed that <i>L. coryniformis</i> WBB05 possesses two putative copies of <i>GlpO</i>, a gene not detected in publicly available genomes of <i>Limosilactobacillus reuteri</i>, which produces reuterin only under anaerobic conditions. These findings support the use of <i>L. coryniformis</i> WBB05 as a safe adjunct culture for dairy applications and highlight <i>GlpO</i> as a candidate determinant of aerobic reuterin production. Further studies comparing <i>GlpO</i>-positive and <i>GlpO</i>-negative strains under aerobic and anaerobic conditions are warranted to confirm the role of <i>GlpO</i>.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.6,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148930076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Margherita Masi, Emanuele Dolfi, Yari Vecchio, Lucia Zambianchi, Ludovica Maria Eugenia Mammi
{"title":"The public perception of animal welfare in dairy cow farming: a systematic literature review approach.","authors":"Margherita Masi, Emanuele Dolfi, Yari Vecchio, Lucia Zambianchi, Ludovica Maria Eugenia Mammi","doi":"10.1017/S002202992610260X","DOIUrl":"https://doi.org/10.1017/S002202992610260X","url":null,"abstract":"<p><p>Animal welfare (AW) in dairy farming has garnered significant public attention, driven by evolving societal values that prioritize ethical treatment and the expression of natural behaviours. This systematic literature review aims to map how the public perceives AW in dairy cow farming. Peer-reviewed publications addressing public perception of dairy cow welfare were retrieved from the Scopus database (searched in May 2025), yielding 107 studies that were examined through bibliometric and network analyses. They were then categorized, according to the perspective they addressed, into three stakeholder spheres: experts (those directly involved in the production chain), citizens and consumers. A dedicated content analysis of 73 documents further examined the citizen and consumer groups across three temporal phases: (i) the 'Lag phase' (up to 2012), marking the topic's emergence in scientific literature; (ii) the 'Naturalness phase' (2013-2019), characterized by consistent emphasis on dairy cows' and calves' right to express natural behaviours; and (iii) the 'Detailed phase' (from 2020), refining and broadening earlier themes. AW perceptions are intrinsically linked to the enabling of natural behaviours (e.g. pasture access, prolonged cow-calf contact), whereas routine practices (e.g. early separation, hormone use, male calf culling) are generally viewed as exploitative. From the consumer's side, a willingness to pay a premium for welfare-assured products emerges, yet an attitude-behaviour gap exists, as citizens' ethical stances may not fully translate into purchasing decisions due to price sensitivity. Notably, AW frequently supersedes environmental sustainability in consumer priorities, while evidence-based communication improves the acceptance of innovations (e.g. genetic modifications for welfare). Being based on a single database and excluding grey literature, the review nonetheless emphasizes the importance of stakeholder collaboration, transparent labelling and policy frameworks that align ethical expectations with farming realities and advocates targeted education to bridge knowledge gaps and support sustainable dairy systems.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-18"},"PeriodicalIF":1.6,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lenira El Faro, Rayanne Casabona Castanheira, Karolini Tenffen De-Sousa, Gustavo Henrique Carvalho Borges, Marcia Ingrid de Paiva, Edilane Aparecida da Silva, João Alberto Negrão, Marcia Saladini Vieira Salles
{"title":"Production and composition of colostrum and milk of Gir cows and their calves' responses to tactile stimulation and feeding plan.","authors":"Lenira El Faro, Rayanne Casabona Castanheira, Karolini Tenffen De-Sousa, Gustavo Henrique Carvalho Borges, Marcia Ingrid de Paiva, Edilane Aparecida da Silva, João Alberto Negrão, Marcia Saladini Vieira Salles","doi":"10.1017/S0022029926102490","DOIUrl":"https://doi.org/10.1017/S0022029926102490","url":null,"abstract":"<p><p>Two complementary experiments were conducted to (1) describe the production and composition of colostrum, transition milk and whole milk in pasture-managed dairy Gir cows and (2) evaluate the effects of tactile stimulation and feeding plan on circulating hormone concentrations in dairy Gir calves. In Experiment 1, we followed Gir cows (<i>n</i> = 38) from calving to 120 days of lactation, and in Experiment 2, we evaluated their calves (<i>n</i> = 37) from birth to 30 days post-weaning. In Experiment 1, milk yield and composition were recorded. In Experiment 2, calves were assigned to four treatments: Conv-NS (conventional feeding, no tactile stimulation), Conv-TS (conventional feeding, tactile stimulation), Opt-NS (optimised feeding, no tactile stimulation) and Opt-TS (optimised feeding, tactile stimulation). Tactile stimulation (TS; gentle hand stroking performed by the handler) was applied twice daily from birth to 60 days old. Both optimised and conventional feeding calves had access to natural suckling during each milking; however, calves in the optimised treatment also received additional milk via a teat bottle after suckling. Calves' circulating hormone concentrations (cortisol and oxytocin) were evaluated during pre-weaning, weaning and post-weaning periods. Cows whose calves were assigned to the Opt-NS produced more residual milk (<i>P</i> ≤ 0.01) but had lower total milk yield compared with cows in the Conv-NS, Conv-TS and Opt-TS. During the post-weaning period, calves in the Conv-TS, Opt-NS and Opt-TS exhibited lower cortisol concentrations (<i>P</i> ≤ 0.05) than those in the Conv-NS (control). Additionally, calves in the Opt-TS showed higher oxytocin concentrations (<i>P</i> = 0.02) during the post-weaning period compared with Conv-NS at pre-weaning. Solid feed intake increased similarly across all treatments throughout the experimental period. In summary, the combination of tactile stimulation and optimised feeding did not impair milk yield in cows, even under systems where calves have access to their dams during milking.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microbiological properties, biogenic amines, total phenolic contents and antioxidant activities of goat kefir fortified with locust bean gum.","authors":"Ercan Sarıca, Gülsüme Bıçakcı, Derya Atalay, Şeyma Betül Encu","doi":"10.1017/S0022029926102647","DOIUrl":"https://doi.org/10.1017/S0022029926102647","url":null,"abstract":"<p><p>Kefir from goat milk is a functional fermented dairy product with probiotics, enhanced digestibility and a distinct nutritional profile. This study aimed to investigate the effects of the addition of different concentrations (0.05%, 0.1% and 0.2%) of locust bean gum (LBG) on some biofunctional and microbiological properties of goat milk kefir during 28 days of storage. Storage time was the predominant factor affecting microbial viability. Lactobacilli, lactococci, total mesophilic aerobic bacteria (TMAB), acetic acid bacteria (AAB) and <i>Leuconostoc</i> spp. decreased mainly during the first 14 days of storage, whereas yeast counts increased throughout storage. LBG supplementation significantly affected lactococci, AAB, yeast populations and tyrosine content, whereas no significant effects were observed for lactobacilli, TMAB and <i>Leuconostoc</i> spp. Tyrosine content ranged from 0.082 to 0.121 mg/g and increased significantly during storage. Kefir samples exhibited antibacterial activity against <i>Listeria monocytogenes, Bacillus cereus</i> and <i>Salmonella</i> Typhimurium. Individual biogenic amine concentrations in kefir samples ranged between 9.72 and 50.08 mg/kg. Although histamine was significantly affected by treatment, the effect was not consistent across LBG inclusion levels. Total phenolic content ranged from 6.39 to 8.74 mg gallic acid equivalents/100 g and increased significantly during storage, whereas antioxidant activity averaged 0.87 ± 0.25 µM Trolox/100 g and was not significantly affected by either LBG supplementation or storage time. Overall, storage time was the primary factor governing changes in goat milk kefir during refrigerated storage, whereas LBG supplementation exerted limited but selective effects depending on the quality parameter evaluated.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of lyophilized soapwort extract on the physicochemical, textural and sensory properties of nonfat yogurt.","authors":"Merve Tunç, Gamze Koçer Alaşalvar, Metin Yildirim","doi":"10.1017/S0022029926102593","DOIUrl":"https://doi.org/10.1017/S0022029926102593","url":null,"abstract":"<p><p>This study investigated the potential use of lyophilized soapwort (<i>Gypsophila</i> sp.) extract as a functional ingredient in set-type nonfat yogurt. Yogurt samples were supplemented with lyophilized soapwort extract at four different concentrations (Control: 0%, 0.025%, 0.050% and 0.100% w/w) and stored for 30 days at 4°C. The effects of the extract on the physicochemical (acidity, dry matter, protein and fat), textural, rheological, microstructural (scanning electron microscopy [SEM]) and sensory properties of the yogurts were evaluated. The addition of soapwort extract did not significantly affect the pH, titratable acidity, total solids, protein content or colour parameters (<i>L*, a*, b*</i>) of the samples. Although the total colour difference (Δ<i>E*</i>) was significantly influenced by the extract, the values remained below the visual detection threshold (Δ<i>E*</i> < 2). Regarding textural properties, the extract did not significantly affect viscosity or serum separation. However, gel hardness decreased significantly with increasing soapwort extract concentration, from 343.8 g in the control sample to 279.7 g in the sample containing the highest level of soapwort extract (0.100%). SEM revealed that the extract interfered with the casein network, leading to an irregular gel structure characterized by larger pores and smaller aggregates compared to the control. Sensory evaluation indicated that while higher concentrations reduced consumer acceptability due to textural changes and bitterness, the sample containing 0.025% extract was statistically comparable to the control. Consequently, 0.025% was identified as the optimum inclusion level. These findings suggest that dried soapwort extract can be successfully incorporated into yogurt at low concentrations to develop a novel functional dairy product.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lianzhe Shi, Akke Kok, Bas Kemp, Ariette van Knegsel
{"title":"Quantifying the 24-h pattern and daily mean behavioural variables of dairy herds related to the environmental temperature and humidity.","authors":"Lianzhe Shi, Akke Kok, Bas Kemp, Ariette van Knegsel","doi":"10.1017/S0022029926102556","DOIUrl":"https://doi.org/10.1017/S0022029926102556","url":null,"abstract":"<p><p>This study addressed the hypothesis that 24-h patterns of behavioural variables of dairy cows for specific periods within a day (i.e., reflecting the 24-h behavioural pattern) are sensitive to increasing daily mean temperature-humidity index (THI) and may provide complementary information to daily mean behavioural variables based on full days. Meteorological data and behavioural sensor data, including lying duration, number of steps and number of postural changes, were retrieved from eight dairy farms. Eighteen behavioural variables were extracted using time windows informed by exploratory analysis across three THI categories. Several 24-h pattern variables reflecting behavioural changes for specific periods in a day had a consistent direction of correlation with the daily mean THI across farms on days with and/or without pasture access. For the daily mean variables, only the daily mean lying duration had a consistent direction of correlation with daily mean THI across farms on days without pasture access, and no daily mean variables had a consistent direction of correlation with daily mean THI across farms on days with pasture access. Generalized additive mixed models indicated that relationships between behavioural variables and daily mean THI were nonlinear. For most behavioural variables, the daily mean THI ranges in which the behavioural variables changed differed between days with and without pasture access. Some of the 24-h pattern behavioural variables exhibited a wider daily mean THI range with significant behavioural change than the daily mean variables. In conclusion, some of the variables derived from the 24-h behavioural pattern were highly sensitive to daily mean THI and could complement daily mean variables in the detection of heat stress. Dairy cows had more behavioural changes in response to increasing THI on days with pasture access than on days without pasture access.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hatice Sena Olcay, Meral Yildirim-Yalcin, Fatih Tarlak
{"title":"Impact of synbiotic formulation on lactic acid bacteria viability and quality attributes of yogurt during cold storage.","authors":"Hatice Sena Olcay, Meral Yildirim-Yalcin, Fatih Tarlak","doi":"10.1017/S0022029926102623","DOIUrl":"https://doi.org/10.1017/S0022029926102623","url":null,"abstract":"<p><p>This study evaluated the impact of synbiotic formulation on lactic acid bacteria (LAB) viability and quality attributes of yogurt during cold storage. Yogurt was manufactured with traditional starter cultures (<i>Lactobacillus delbrueckii</i> subsp. <i>bulgaricus</i> and <i>Streptococcus thermophilus</i>) with or without <i>Lactobacillus acidophilus</i> and enriched or not with freeze-dried banana powder, yielding three formulations: (i) conventional, (ii) probiotic and (iii) synbiotic. After fermentation at 41.5 °C to pH 4.7 ± 0.2, samples were stored at 4 °C and analysed on days 1, 7, 14 and 21 for LAB counts, physicochemical parameters (pH, titratable acidity, protein, moisture, ash, serum separation), total phenolic content, viscosity and sensory properties. The synbiotic formulation supported LAB viability throughout storage, while the probiotic formulation exhibited the highest LAB counts at the end of the storage period. However, the addition of banana powder also intensified post-acidification due to enhanced microbial metabolism, leading to lower pH and higher titratable acidity. From a structural perspective, interactions between banana-derived components and the casein network modified the gel microstructure, resulting in increased syneresis and reduced viscosity. These physicochemical changes were reflected in sensory properties, with the synbiotic yogurt showing slightly lower scores despite its functional benefits. In contrast, probiotic yogurt received the highest sensory scores in the trained panel evaluation. Overall, the findings demonstrate that banana-based prebiotic enrichment can support LAB viability and functional properties of yogurt but also induces structure-related changes that influence texture and stability. These results suggest that optimisation of ingredient interactions is important for balancing functionality and product quality in synbiotic dairy systems.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.6,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148684830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}