Journal of Dairy Research最新文献

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Effects of calcium chloride substitution on the physicochemical properties of Minas Frescal cheese. 氯化钙取代对米纳斯奶酪理化性质的影响。
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/S0022029923000316
Fernanda Lopes da Silva, Andressa Fusieger, Maria Tereza Cratiú Moreira, Isabelle Carolina Oliveira, Ítalo Tuler Perrone, Naaman Francisco Nogueira Silva, Rodrigo Stephani, Antônio Fernandes de Carvalho
{"title":"Effects of calcium chloride substitution on the physicochemical properties of Minas Frescal cheese.","authors":"Fernanda Lopes da Silva,&nbsp;Andressa Fusieger,&nbsp;Maria Tereza Cratiú Moreira,&nbsp;Isabelle Carolina Oliveira,&nbsp;Ítalo Tuler Perrone,&nbsp;Naaman Francisco Nogueira Silva,&nbsp;Rodrigo Stephani,&nbsp;Antônio Fernandes de Carvalho","doi":"10.1017/S0022029923000316","DOIUrl":"https://doi.org/10.1017/S0022029923000316","url":null,"abstract":"<p><p>The aim in this research paper was to investigate the effect of using calcium monophosphate (MCP) and MCP mixed with commercial phosphates salts, in total or partial replacement of calcium chloride (CaCl<sub>2</sub>) in the manufacture of Minas Frescal cheese. Initially, model cheeses were made to perform the rheological analysis during the coagulation process. Of these, the five best treatments were chosen to carry out the production of Minas Frescal cheese, used only CaCl<sub>2</sub> and MCP, and partial replacements of MCP + polyphosphate, MCP + potassium monophosphate (MKP) and MCP. The cheeses showed no significant difference in physicochemical composition, yield and syneresis, however, the cheese with partial replacement of CaCl<sub>2</sub> by MCP + polyphosphate and MCP + MKP showed the highest hardness values, like the control. This demonstrates that it is possible to replace calcium chloride without significant changes in the physicochemical characteristics and yield of Minas Frescal cheese, and it is still possible to modulate the hardness of the cheese produced according to the type of calcium/phosphate source used. This allows the industry to replace the source of calcium in the manufacture of Minas Frescal cheese according to the desired hardness.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9977950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of different production methods on quality parameters of Hatay Künefe cheese. 不同生产方法对哈塔伊k<s:1>奈菲奶酪品质参数的影响。
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/S0022029923000298
Nuray Güzeler, Ahmet Refik Çay, Firuza Koboyeva, Mustafa Kadir Esen
{"title":"Effect of different production methods on quality parameters of Hatay Künefe cheese.","authors":"Nuray Güzeler,&nbsp;Ahmet Refik Çay,&nbsp;Firuza Koboyeva,&nbsp;Mustafa Kadir Esen","doi":"10.1017/S0022029923000298","DOIUrl":"https://doi.org/10.1017/S0022029923000298","url":null,"abstract":"<p><p>We investigated four different production methods of Künefe cheese, emphasizing their industrial importance. The four methods used fresh Künefe cheese (FKC), salted Künefe cheese (SKC), Boru type Künefe cheese (BKC) and culture-added processed Künefe cheese (CPKC), all used in the production of Künefe, a syrupy dessert unique to the Middle East. FKC was produced as a result of curd formation from raw milk with rennet and then the fermentation of curd. SKC was manufactured using the salting method in addition to FKC production. BKC was obtained using the dry cooking process with emulsifying salts applied to cheese curd. CPKC was produced by applying heat treatment to raw milk and using starter culture before adding rennet that was different to Boru-type Künefe cheese. The composition, color, meltability, texture and sensory properties of the Künefe cheeses were examined. As a result of statistical analysis, it was determined that the effect of different production methods on the composition, meltability, texture (except springiness and cohesiveness) and sensory properties was significant in all cheeses (<i>P</i> < 0.05). CKPC was the most appropriate cheese in various properties.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9920395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advances in sustainable dairy cattle nutrition 奶牛可持续营养研究进展
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/s0022029923000390
C. H. Knight
{"title":"Advances in sustainable dairy cattle nutrition","authors":"C. H. Knight","doi":"10.1017/s0022029923000390","DOIUrl":"https://doi.org/10.1017/s0022029923000390","url":null,"abstract":"","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45823577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DAR volume 90 issue 2 Cover and Back matter DAR第90卷第2期封面和封底
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/s0022029923000419
{"title":"DAR volume 90 issue 2 Cover and Back matter","authors":"","doi":"10.1017/s0022029923000419","DOIUrl":"https://doi.org/10.1017/s0022029923000419","url":null,"abstract":"","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49106991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seasonal, physiological and bacteriological risk factors for subclinical mastitis in dairy cows maintained under different farming conditions. 不同饲养条件下奶牛亚临床乳腺炎的季节、生理和细菌危险因素。
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/S0022029923000389
Deepak Sharma, Sankar Kaniamuthan, Ayyasamy Manimaran, Arumugam Kumaresan, Muniandy Sivaram, Duraisamy Rajendran, Pratik Ramesh Wankhade, Veerasamy Sejian, Sughra Banu
{"title":"Seasonal, physiological and bacteriological risk factors for subclinical mastitis in dairy cows maintained under different farming conditions.","authors":"Deepak Sharma,&nbsp;Sankar Kaniamuthan,&nbsp;Ayyasamy Manimaran,&nbsp;Arumugam Kumaresan,&nbsp;Muniandy Sivaram,&nbsp;Duraisamy Rajendran,&nbsp;Pratik Ramesh Wankhade,&nbsp;Veerasamy Sejian,&nbsp;Sughra Banu","doi":"10.1017/S0022029923000389","DOIUrl":"https://doi.org/10.1017/S0022029923000389","url":null,"abstract":"<p><p>Subclinical mastitis (SCM) is a major health problem of dairy animals in India and across the globe. An identification of potential risk factors of SCM can help for efficient udder health management in dairy animals. In this study, apparently healthy cows (HF crossbred: <i>n</i> = 45; Deoni: <i>n</i> = 43) were screened for SCM during different seasons through milk somatic cell count (SCC: reference test using 200 × 10<sup>3</sup> cells/ml as cut off value), California mastitis test (CMT) and differential electrical conductivity (DEC) test at an organized research farm. SCM positive milk samples (<i>n</i> = 34) were inoculated in selective media for <i>Coliform</i> sp., <i>Streptococcus</i> sp. and <i>Staphylococcus</i> sp. and DNA was isolated (<i>n =</i> 10) for species confirmation by 16s rRNA method. Both bivariate and multivariate models were used for risk assessment. We found the cumulative prevalence of 31 and 65% SCM in Deoni and crossbred cows, respectively. Screening of 328 crossbred cows under field conditions revealed point prevalence of 55% SCM. Multivariate analysis revealed stage of lactation (SOL), milk yield in previous lactation and test day milk yield in Deoni cows, as well as parity and mastitis treatment history in current lactation in HF crossbred cows as risk factors. SOL was a significant factor under field conditions. Receiver operated characteristic curve analysis revealed better accuracy of CMT than DEC. We found more mixed infections due to <i>Staphylococcus</i> sp. and <i>Streptococcus</i> sp. in culture, while 16s rRNA based molecular method revealed lesser-known pathogens associated with SCM. It is concluded that SCM prevalence rate is higher in crossbred than indigenous cows and these breeds have different risk factors for SCM. HF crossbred cows had similar SCM prevalence rate under different farming conditions, where CMT can be used for SCM diagnosis with excellent accuracy. The 16s rRNA method is useful for specific identification of lesser known and emerging mastitis pathogens.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9920954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predicting live weight using body volume formula in lactating water buffalo. 利用体体积公式预测哺乳期水牛的活重。
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/S0022029923000249
Remedio Ramos-Zapata, Camila Dominguez-Madrigal, Ricardo-A García-Herrera, Enrique Camacho-Perez, Jesús Manuel Lugo-Quintal, Thobela Louis Tyasi, Antonio Leandro Chaves Gurgel, Luís Carlos Vinhas Ítavo, Alfonso Juventino Chay-Canul
{"title":"Predicting live weight using body volume formula in lactating water buffalo.","authors":"Remedio Ramos-Zapata,&nbsp;Camila Dominguez-Madrigal,&nbsp;Ricardo-A García-Herrera,&nbsp;Enrique Camacho-Perez,&nbsp;Jesús Manuel Lugo-Quintal,&nbsp;Thobela Louis Tyasi,&nbsp;Antonio Leandro Chaves Gurgel,&nbsp;Luís Carlos Vinhas Ítavo,&nbsp;Alfonso Juventino Chay-Canul","doi":"10.1017/S0022029923000249","DOIUrl":"https://doi.org/10.1017/S0022029923000249","url":null,"abstract":"<p><p>Live weight (LW) is an important piece of information within production systems, as it is related to several other economic characteristics. However, in the main buffalo-producing regions in the world, it is not common to periodically weigh the animals. We develop and evaluate linear, quadratic, and allometric mathematical models to predict LW using the body volume (BV) formula in lactating water buffalo (<i>Bubalus bubalis</i>) reared in southeastern Mexico. The LW (391.5 ± 138.9 kg) and BV (333.62 ± 58.51 dm<sup>3</sup>) were measured in 165 lactating Murrah buffalo aged between 3 and 10 years. The goodness-of-fit of the models was evaluated using the Akaike Information Criterion (AIC), Bayesian Information Criterion (BIC), coefficient of determination (<i>R</i><sup>2</sup>), mean-squared error (MSE) and root MSE (RMSE). In addition, the developed models were evaluated through cross-validation (<i>k</i>-folds). The ability of the fitted models to predict the observed values was evaluated based on the RMSEP, <i>R</i><sup>2</sup>, and mean absolute error (MAE). LW and BV were significantly positively and strongly correlated (<i>r</i> = 0.81; <i>P</i> < 0.001). The quadratic model had the lowest values of MSE (2788.12) and RMSE (52.80). On the other hand, the allometric model showed the lowest values of BIC (1319.24) and AIC (1313.07). The Quadratic and allometric models had lower values of MSEP and MAE. We recommend the quadratic and allometric models to predict the LW of lactating Murrah buffalo using BV as a predictor.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9925624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Full-fat corn germ in diets for dairy cows as an alternative to ground corn. 乳牛日粮中全脂玉米胚芽替代玉米粉的研究。
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/S0022029923000201
Antônio J Netto, Marco A S da Gama, Sebastião I Guido, Jonas G Inácio, Juana C C Chagas, Mohammad Ramin, Milena N Rabelo, Silas B Félix, Camila S da Silva, Marcelo de A Ferreira
{"title":"Full-fat corn germ in diets for dairy cows as an alternative to ground corn.","authors":"Antônio J Netto,&nbsp;Marco A S da Gama,&nbsp;Sebastião I Guido,&nbsp;Jonas G Inácio,&nbsp;Juana C C Chagas,&nbsp;Mohammad Ramin,&nbsp;Milena N Rabelo,&nbsp;Silas B Félix,&nbsp;Camila S da Silva,&nbsp;Marcelo de A Ferreira","doi":"10.1017/S0022029923000201","DOIUrl":"https://doi.org/10.1017/S0022029923000201","url":null,"abstract":"<p><p>The experiments reported in this research paper address the effects of replacing ground corn (GC) with full-fat corn germ (FFCG) on nutrient intake and digestibility, nitrogen utilization efficiency, performance, and predicted methane production in dairy cows fed cactus cladodes and sugarcane. We hypothesized that the inclusion of FFCG in the diet would not alter the performance of lactating cows but would reduce the predicted methane production in vivo. Ten multiparous Holstein cows at 90 ± 10 d of lactation and yielding 24.2 ± 3.5 kg milk/d were assigned to dietary treatments consisting of different levels of replacement of GC by FFCG (0; 25; 50; 75 and 100% of diet dry matter) in a replicated 5 × 5 Latin square design with 21-d periods. Methane production was predicted using an automated gas in vitro production system. Except for ether extract intake, which increased, the intake of all nutrients decreased linearly with the replacement of GC by FFCG. The digestibility of dry matter, organic matter and neutral detergent fiber reduced, whereas the digestibility of ether extract increased linearly with FFCG. There were no changes in the digestibility of crude protein. The nitrogen intake and daily excretion in urine and feces decreased, while nitrogen use efficiency increased linearly. There was no significant effect of diets on nitrogen balance or microbial protein synthesis and efficiency. The yield of protein, lactose and total solids in milk showed a quadratic behavior. On the other hand, milk fat yield and energy-corrected milk yield decreased linearly with the replacement of GC by FFCG. No effect on pH or ammonia nitrogen was observed. The production of methane (CH<sub>4</sub>, g/kg DM) and total CH<sub>4</sub> (g/d), and CH<sub>4</sub> intensity decreased linearly with the replacement of GC by FFCG. In conclusion, FFCG has been shown to be an effective source of fat to reduce methane production in dairy cows, partially supporting our initial hypothesis. However, as it decreases milk fat production, it is not recommended to replace more than 50% of GC by FFCG for lactating cows fed cactus cladodes and sugarcane.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9923016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DAR volume 90 issue 2 Cover and Front matter DAR第90卷第2期封面和封面
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/s0022029923000407
{"title":"DAR volume 90 issue 2 Cover and Front matter","authors":"","doi":"10.1017/s0022029923000407","DOIUrl":"https://doi.org/10.1017/s0022029923000407","url":null,"abstract":"","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47609680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathogens in milk of goats and their relationship with somatic cell count. 山羊乳中病原菌及其与体细胞数的关系。
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/S0022029923000237
Kristína Tvarožková, Vladimír Tančin, Michal Uhrinčať, Marta Oravcová, Lukáš Hleba, Barbora Gancárová, Lucia Mačuhová, Martin Ptáček, Pierre-Guy Marnet
{"title":"Pathogens in milk of goats and their relationship with somatic cell count.","authors":"Kristína Tvarožková,&nbsp;Vladimír Tančin,&nbsp;Michal Uhrinčať,&nbsp;Marta Oravcová,&nbsp;Lukáš Hleba,&nbsp;Barbora Gancárová,&nbsp;Lucia Mačuhová,&nbsp;Martin Ptáček,&nbsp;Pierre-Guy Marnet","doi":"10.1017/S0022029923000237","DOIUrl":"https://doi.org/10.1017/S0022029923000237","url":null,"abstract":"<p><p>We evaluated the presence of bacterial pathogens in the milk of goats and their relationship with somatic cell count (SCC) and milk composition. The study was performed on a dairy farm in northern Slovakia. Half udder milk samples were collected from goats in June and July. The samples were divided on the basis of SCC into 4 bands (SCC1 lowest to SCC4 highest). Bacterial pathogens were only detected in 13% of samples. SCC3 and SCC4 had 15 and 25% positive samples respectively compared with SCC1 (2%) and SCC2 (14%). Coagulase-negative staphylococci (CNS) were the most common isolates (73%), of which <i>Staphylococcus caprae</i> was the most frequently isolated (65%). In samples with ≥ 1000 × 10<sup>3</sup> cells ml<sup>- 1</sup> (SCC3, SCC4) there was higher somatic cell score (SCS) in the presence of a pathogen (7.48 ± 0.11) than without a pathogen (7.16 ± 0.05, <i>P</i> < 0.01). Statistically significant but weak negative correlations were observed between SCS and lactose, dry matter and non-fat dry matter. In conclusion, a higher percentage of bacteriologically positive milk samples was observed in both SCC3 and SCC4 groups but this does not explain the aetiology of high SCC in the milk of goats that are apparently free of bacteria. As a diagnostic tool, SCC is probably less useful in goats than in cows.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10280307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of conceptus stimulated gene expression in buffalo peripheral blood mononuclear cells as potential diagnostic markers of early pregnancy. 水牛外周血单核细胞受孕刺激基因表达作为早期妊娠潜在诊断标志物的研究。
IF 2.1 3区 农林科学
Journal of Dairy Research Pub Date : 2023-05-01 DOI: 10.1017/S0022029923000304
Paramjeet Sharma, Ratan Kumar Choudhary, Navdeep Singh Ratta, Sikh Tejinder Singh
{"title":"Investigation of conceptus stimulated gene expression in buffalo peripheral blood mononuclear cells as potential diagnostic markers of early pregnancy.","authors":"Paramjeet Sharma,&nbsp;Ratan Kumar Choudhary,&nbsp;Navdeep Singh Ratta,&nbsp;Sikh Tejinder Singh","doi":"10.1017/S0022029923000304","DOIUrl":"https://doi.org/10.1017/S0022029923000304","url":null,"abstract":"<p><p>Exploration of novel strategies for early pregnancy diagnosis is pivotal in enhancing the reproductive potential and monetary gains from dairy herds. In buffalo, the trophectoderm cells of the elongating conceptus secrete interferon-tau that stimulates the transcription of various genes in peripheral blood mononuclear cells (PBMC) during the peri-implantation period. We explored the differential expression of classical (<i>ISG15</i>) and novel (<i>LGALS3BP</i> and <i>CD9</i>) early pregnancy markers in PBMC of buffaloes during various stages of pregnancy. Natural heat was detected in buffaloes by assessing the vaginal fluid, and artificial insemination (AI) was done. Whole blood was collected from the jugular vein in EDTA-containing vacutainers for PBMC isolation before AI (0-day) and 20, 25 and 40 d post-AI. On day 40, transrectal ultrasonography examination was performed to confirm pregnancy. The inseminated non-pregnant animals served as control. Total RNA was extracted using the TRIzol method. The temporal abundance of <i>ISG15, LGALS3BP</i> and <i>CD9</i> genes in PBMC was compared between pregnant and non-pregnant groups (<i>n</i> = 9 per group) using real time-qPCR. Results showed transcripts of <i>ISG15</i> and <i>LGALS3BP</i> were more abundant at 20 d in the pregnant group compared to the 0 d and 20 d values of the non-pregnant group. However, due to variability in expression, threshold (Ct) cycle of RT-qPCR alone could not distinguish pregnant and non-pregnant animals. In conclusion, <i>ISG15</i> and <i>LGALS3BP</i> transcripts abundance in PBMCs are potential candidate biomarkers for early prediction of buffalo pregnancy 20-days post-AI, but further work is required to allow the development of a reliable new methodology.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9920391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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