Journal of Dairy Research最新文献_第3页

Development and validation of a novel HPLC-PDA method for the detection of preservatives in milk. HPLC-PDA检测牛奶中防腐剂的新方法的建立与验证。

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 Epub Date: 2025-03-06 DOI: 10.1017/S0022029925000044

Natalia Gonzaga, Lycio Shinji Watanabe, Samanta Stinghen de Abreu, Stael Málaga Carrilho, Fernanda Montanholi de Lira, Ronaldo Tamanini, Suzana Lucy Nixdorf, Vanerli Beloti

{"title":"Development and validation of a novel HPLC-PDA method for the detection of preservatives in milk.","authors":"Natalia Gonzaga, Lycio Shinji Watanabe, Samanta Stinghen de Abreu, Stael Málaga Carrilho, Fernanda Montanholi de Lira, Ronaldo Tamanini, Suzana Lucy Nixdorf, Vanerli Beloti","doi":"10.1017/S0022029925000044","DOIUrl":"10.1017/S0022029925000044","url":null,"abstract":"In this research paper we introduce and validate an enhanced method for the detection of sodium benzoate and potassium sorbate in milk. Sodium benzoate and potassium sorbate are widely employed as preservatives in the food industry; however, their use to restrict microbial growth in milk is prohibited. Our novel method achieved performance indicators in accordance with the criteria outlined by the International Conference on Harmonization. The extraction process involved sample dilution in acetonitrile (1:1 v/v), followed by subsequent centrifugation, filtration and injection into a high-performance liquid chromatography system with a photodiode array detector. The detection and quantification limits for sodium benzoate were determined to be 0.204 and 0.618 mg/l, respectively, while for potassium sorbate, these values were 0.108 and 0.328 mg/l, respectively. The accuracy ranged from 92.67 to 99.53%, with pH 4.0 selected as the optimal condition to ensure adequate resolution of the preservatives. The proposed method stands out due to its simplicity and speed, using a single reagent in modest quantities during sample preparation. This approach reduces toxicity and minimizes the production of pollutants during disposal. Furthermore, the novel method requires less raw material and energy consumption, aligning with the principles of green chemistry. Its lower quantitation limits render it more sensitive when compared to the official analysis. The preparation, separation, and simultaneous detection of these preservatives in a short period of time make this method suitable for integration into industrial workflows.","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"465-470"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cross-continental animal science perspective on milk fat research: what has happened and where are we heading? 跨洲动物科学视角下的乳脂研究：发生了什么？我们将走向何方？

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 Epub Date: 2025-05-19 DOI: 10.1017/S002202992500007X

Einar Vargas-Bello-Pérez, Manuel Gonzalez-Ronquillo, Navid Ghavipanje, Naifeng Zhang, Pilar Gómez-Cortés, Heidi Leskinen, David Pacheco, Mohammad Ashiqul Islam, Ahmed E Kholif, Marcello Mele, Juan J Loor, Kirsty E Kliem

{"title":"A cross-continental animal science perspective on milk fat research: what has happened and where are we heading?","authors":"Einar Vargas-Bello-Pérez, Manuel Gonzalez-Ronquillo, Navid Ghavipanje, Naifeng Zhang, Pilar Gómez-Cortés, Heidi Leskinen, David Pacheco, Mohammad Ashiqul Islam, Ahmed E Kholif, Marcello Mele, Juan J Loor, Kirsty E Kliem","doi":"10.1017/S002202992500007X","DOIUrl":"10.1017/S002202992500007X","url":null,"abstract":"Milk is a fundamental food matrix that is widely consumed. Milk fat is important for producing dairy products such as butter, cream, cheese and whole milk powder. Aside from flavour, it has been linked to human health and its chemistry can be modulated by various means towards a more healthy fatty acid profile. Industry and stakeholders have different interests in milk fat, based on specific policies which reflect the type of research and funding initiatives currently performed in different countries. This position paper summarizes the current state-of-the-art with regards to milk fat research and industry as well as stakeholder initiatives, and then highlights new developments based on information gathered from North America (United States and Mexico), Europe (United Kingdom, Spain, Italy, and Finland), Africa (Egypt), Asia (China and Bangladesh) and Oceania (New Zealand). South America is an important contributor to the dairy industry but will not be considered here and thus this paper must be considered cross-continental rather than global. This manuscript intends to show a wide 'picture' of milk fat from different angles in different parts of the globe.","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":"91 4","pages":"445-453"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of parity number, milk production and somatic cell count on the reproduction of Holstein cows - ERRATUM. 奇数、产奶量和体细胞数对荷斯坦奶牛繁殖的影响 - ERRATUM。

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 DOI: 10.1017/S0022029924000177

Bianca Letícia Barbosa, Victor Breno Pedrosa, Luciana da Silva Leal Karolewski, Caio César de Godoi, Adriana de Souza Martins

引用次数: 0

Sire association with intramammary infection and clinical mastitis in a Holstein-Friesian × Jersey crossbred pedigree herd. 在荷斯泰因-弗里西亚×泽西杂交纯种群体中，父系与乳腺内感染和临床乳腺炎的关系。

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 Epub Date: 2025-05-19 DOI: 10.1017/S0022029924000682

S Jane Lacy-Hulbert, Sally-Anne Turner, Barbara Kuhn-Sherlock, John H Williamson, Richard J Spelman, J Eric Hillerton

{"title":"Sire association with intramammary infection and clinical mastitis in a Holstein-Friesian × Jersey crossbred pedigree herd.","authors":"S Jane Lacy-Hulbert, Sally-Anne Turner, Barbara Kuhn-Sherlock, John H Williamson, Richard J Spelman, J Eric Hillerton","doi":"10.1017/S0022029924000682","DOIUrl":"https://doi.org/10.1017/S0022029924000682","url":null,"abstract":"This research paper describes a test of association of sire with susceptibility to mastitis, using a custom-bred population of dairy cattle. We hypothesised that sire daughters ranked as more resistant to intramammary infections in their first two lactations would be more resistant to an intramammary challenge in their third lactation. Mastitis phenotypes were generated for a Holstein-Friesian × Jersey crossbred research herd of 864 cows, bred from six defined sires and managed as two cohorts in a seasonal calving system in New Zealand. Naturally occurring new intramammary infections (IMI) and clinical mastitis (CM) were monitored in their first two lactations from herd records, milking staff observations and bacteriology of quarter milk samples collected at four time-points during each lactation. The animals retained to their third lactation were then exposed to a single intramammary challenge with Streptococcus uberis. We used a relative risk (RR) analysis to rank performance of sire daughters for pathogen-specific phenotypes for new IMI and CM, and somatic cell count (SCC) traits, and their clinical outcomes to the challenge. Generally, daughters of sire B had the highest RR for new IMI or CM by a major pathogen, whereas daughters of sires A and C had a consistently lower risk. The RR for sires E, D and F were intermediate and inconsistent across major pathogens. Daughters of sire B ranked highest for all CM cases and SCC traits whereas sires A and C ranked lowest. Following intramammary challenge, daughters of sires A and C were more likely to develop CM, whereas daughters of sire B and F were less likely to develop CM. Thus, the hypothesis was rejected. The results revealed strong associations between sire and pathogen-specific mastitis phenotypes, and validated use of SCC and CM traits in sire selection and breeding programmes to improve mastitis resistance.","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":"91 4","pages":"410-419"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of the CACNA2D1 gene with milk yield and milk quality traits in Holstein cattle. CACNA2D1基因与荷斯坦牛产奶量及品质性状的关系。

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 Epub Date: 2025-05-19 DOI: 10.1017/S0022029925000056

Fadime Daldaban, Jale Metin Kıyıcı, Bilal Akyüz, Esma Gamze Aksel, Mahmut Kaliber, Mehmet Ulaş Çınar, Korhan Arslan

{"title":"Association of the CACNA2D1 gene with milk yield and milk quality traits in Holstein cattle.","authors":"Fadime Daldaban, Jale Metin Kıyıcı, Bilal Akyüz, Esma Gamze Aksel, Mahmut Kaliber, Mehmet Ulaş Çınar, Korhan Arslan","doi":"10.1017/S0022029925000056","DOIUrl":"https://doi.org/10.1017/S0022029925000056","url":null,"abstract":"The aim of this study was to investigate the association of the rs43389227, rs43390124 and rs482883241 single-nucleotide polymorphisms (SNPs) of the bovine calcium channel, voltage-dependent, alpha-2/delta subunit 1 (CACNA2D1) gene with milk yield and quality traits in Holstein cattle. For this purpose, 166 Holstein dairy cows were genotyped for these three SNPs. Correlation analyses showed that the rs43389227 SNP was associated with 305-day milk yield (P < 0.05). Besides, rs43390124 SNP was found to be associated not only with 305-day milk yield but also with milk fat, milk quality traits, somatic cell score, electrical conductivity and pH (P < 0.05). The results of this study suggest that the rs43389227 and rs43390124 SNPs could be used in breeding for the improvement of milk yield and milk quality in cattle. Furthermore, based on its association with mastitis related traits, the rs43390124 SNP was considered as a potential indicator of mastitis susceptibility.","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":"91 4","pages":"373-377"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipoproteolytic deteriorating potential and aprX gene of Pseudomonas spp. in raw goat milk. 生羊奶中假单胞菌的脂蛋白降解潜能及aprX基因。

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 Epub Date: 2025-03-05 DOI: 10.1017/S0022029925000147

Samera Rafaela Bruzaroski, Selma de Souza Correia, Karla Eliza de Araújo, Luisa Aragon Alegro, Fabiola Cristine de Almeida Rego Grecco, Elsa Helena Walter de Santana, Ricardo César Tavares Carvalho

{"title":"Lipoproteolytic deteriorating potential and aprX gene of Pseudomonas spp. in raw goat milk.","authors":"Samera Rafaela Bruzaroski, Selma de Souza Correia, Karla Eliza de Araújo, Luisa Aragon Alegro, Fabiola Cristine de Almeida Rego Grecco, Elsa Helena Walter de Santana, Ricardo César Tavares Carvalho","doi":"10.1017/S0022029925000147","DOIUrl":"10.1017/S0022029925000147","url":null,"abstract":"This Research Communication describes the frequency of occurrence of P. fluorescens, P. putida and P. aeruginosa in raw goat milk stored at 4 and 9°C, to evaluate the spoilage potential and capacity of these species and the presence of the aprX gene, responsible for the synthesis of metalloprotease aprX. Samples of raw goat milk stored at 4 and 9°C for 72 h were plated on Pseudomonas CFC agar base (25°C for 48 h). The presence of P. fluorescens, P. putida, P. aeruginosa and aprX gene were confirmed by polymerase chain reaction. The isolates were evaluated for their proteolytic and lipolytic spoilage capacity and potential using milk agar (10%) and tributyrin agar base (1%), respectively (21°C for 72 h). A total of 184 strains of Pseudomonas spp. were obtained. At both temperatures, P. fluorescens was the most frequent, mostly proteolytic, and the only one to express high proteolytic and lipolytic potentials. Regardless of the temperature, all isolated strains of P. putida were lipoproteolytic, with a predominance of protease synthesis. Strains of P. aeruginosa (4,3%) were isolated occasionally (4.3% of total isolates) but only at 4°C, and 75% of these were lipoproteolytic. The storage temperature of raw goat milk influenced the frequency of P. aeruginosa, but not its spoilage potential. The presence of the aprX gene was verified in most strains isolated from all species at the two temperatures evaluated, although some of these strains did not express proteolytic capacity. Most isolates of Pseudomonas spp. showed lipoproteolytic capacity, which emphasizes the importance of ensuring low initial populations of this genus in raw chilled goat milk when it is used to produce dairy products.","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"486-489"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of enterocin from Enterococcus durans MF5 in controlling Listeria species. durans肠球菌MF5肠球菌素控制李斯特菌的潜力。

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 DOI: 10.1017/S0022029925000160

Nathália Aparecida Andrade de Souza, Luana de Carvalho, Matheus Henrique Nogueira, Márcia Cristina Furlaneto, Luciana Furlaneto Maia

{"title":"Potential of enterocin from Enterococcus durans MF5 in controlling Listeria species.","authors":"Nathália Aparecida Andrade de Souza, Luana de Carvalho, Matheus Henrique Nogueira, Márcia Cristina Furlaneto, Luciana Furlaneto Maia","doi":"10.1017/S0022029925000160","DOIUrl":"10.1017/S0022029925000160","url":null,"abstract":"This research paper presents the characterization of an enterocin-producing Enterococcus durans MF5 isolate and the determination of the in vitro antilisterial activity of enterocin produced by this isolate, named Ent-MF5. PCR-based screening for bacteriocin biosynthetic genes revealed that E. durans MF5 harbors multiple enterocin-encoding genes (ent A, B, P and X), classified as class II bacteriocins and enterocin-P of Enterococcus faecium (sharing up to 99% similarity at the genetic level). E. durans MF5 is sensitive to eight clinically important antibiotics and does not possess cytolysin activator -cylA, gelatinase -gelE and hyaluronidase -hylA virulence genes. The antilisterial activity of Ent-MF5 was abolished by trypsin, α-chymotrypsin, protease and proteinase-K. Ent-MF5 showed thermal and pH stability. In addition, the activity of Ent-MF5 was unaffected in the presence of various surfactants (1% SDS, Triton X-100, Tween 20, and Tween 80). Ent-MF5 exhibited antimicrobial activity against Listeria monocytogenes, Listeria innocua, Listeria ivanovii and Listeria seeligeri at concentrations as low as 0.13 μg/ml. Ent-MF5 had a bactericidal effect against L. monocytogenes with a significant reduction in surviving cells at concentrations equal to or greater than 0.13 μg/ml. A 75-100% reduction in L. monocytogenes growth and bactericidal effect determined by CFU counts was observed following treatment with Ent-MF5 at 4.47 μg/ml at time points starting at 2 and 4 h, respectively. Ent-MF5 action is associated with Listeria cell membrane damage, as observed by flow cytometry and fluorescence microscopy. Thus, the effective antilisterial activity and stability of Ent-MF5 presents promising perspectives for application as biopreservatives in the food industry.","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"516-524"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

It's milk, Jim, but not as we know it! 这是牛奶，吉姆，但不是我们所知道的那样！

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 Epub Date: 2025-05-19 DOI: 10.1017/S0022029925000226

Christopher H Knight

引用次数: 0

Removing dietary sodium bicarbonate increases rumination time but does not affect intake or milk yield. 去除饲料中的碳酸氢钠增加了反刍时间，但不影响摄入量或产奶量。

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 Epub Date: 2025-05-19 DOI: 10.1017/S0022029925000159

Daniel Espinoza, Eyal Frank, Yuri Portnik, Yoav Shaani, Yehoshav A Ben Meir

{"title":"Removing dietary sodium bicarbonate increases rumination time but does not affect intake or milk yield.","authors":"Daniel Espinoza, Eyal Frank, Yuri Portnik, Yoav Shaani, Yehoshav A Ben Meir","doi":"10.1017/S0022029925000159","DOIUrl":"https://doi.org/10.1017/S0022029925000159","url":null,"abstract":"We investigated the effect of adding 3 levels of sodium bicarbonate (SB: 0, 0.74 and 1.47% of total mixed ration (TMR) dry matter) to a low-roughage TMR (20.8% wheat silage, 9% wheat hay and 2.3% clover hay) on feed intake, production (milk and milk-component yields), whole-tract apparent digestibility, rumen pH and rumination time. After 2 wk of receiving the same TMR, 42 mid-lactation multiparous cows were blocked into groups of 3 according to their dry matter intake and energy-corrected milk (ECM) yield and divided into 3 treatment groups. The experimental rations were fed for 7 wk while monitoring intake, production and rumination. Rumen pH was measured after 6 h without access to feed and 6 h after feeding (at 0600 and 1500 h) every other week, and feces samples were taken for whole-tract apparent digestibility at wk 7. Dietary SB level did not affect intake, ECM yield, digestibility or efficiency. Dietary SB concentration negatively correlated with daily rumination time but did not affect rumen pH measured before or after feeding. Lactating cows may, therefore, increase their rumination time to compensate for lack of buffer but overall, removing SB from a wheat-based, low roughage TMR does not impair intake or milk or milk-component yields.","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":"91 4","pages":"378-385"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of raising dairy calves by foster cows in Mexico. 墨西哥寄养奶牛饲养犊牛的评价。

IF 1.6 3区农林科学

Journal of Dairy Research Pub Date : 2024-11-01 Epub Date: 2025-05-19 DOI: 10.1017/S0022029925000172

David A Contreras, Lena Lidfors, Carlos S Galina, Benjamín García, Carlos E Hernández

{"title":"Evaluation of raising dairy calves by foster cows in Mexico.","authors":"David A Contreras, Lena Lidfors, Carlos S Galina, Benjamín García, Carlos E Hernández","doi":"10.1017/S0022029925000172","DOIUrl":"https://doi.org/10.1017/S0022029925000172","url":null,"abstract":"Our aim was to evaluate a foster cow-calf rearing system on the adoption or acceptance of fostered calves, milk production and udder health, as well as calf health and weight gain, and to compare this fostering system to traditional rearing. The foster group (FG) consisted of 8 cows each suckling 3 fostered calves with continuous contact. The control group (CG) was a conventional milking system, whereby cows and control calves were kept separate. The duration of the experiment was 8 weeks. Behavioural observations were carried out after the calves were introduced to the FG to find out if and how many calves were adopted or accepted (complete or incomplete maternal behaviour expressed, respectively). Milk production (let down) was recorded daily for CG and once a week for FG (after 8-h of no suckling). Milk samples were collected once a week from both groups for California mastitis test, Wisconsin test, and somatic cell count. A daily record of the incidence of diarrhoea was made on the calves and they were weighed once a week. Results showed that six of the eight FC cows had adopted the three calves, whilst one adopted two calves and accepted one and one adopted one calf and accepted two. One other cow refused all three calves from the outset and was removed from the study. Milk production at a single milking was 2.52 ± 1.04 (mean ± sd) 10.07 ± 0.76 l for FG and CG, respectively. Udder health improved over time in FG as evidenced by a progressive reduction in SCC. The average weight gain for FG calves was higher than for CG (700.7 ± 97.7 vs. 471 ± 188.7 g/d). In conclusion, the foster-cow rearing system was well received by most cows, the udder health on FG showed an improvement in comparison to the CG, and a higher weight gain was found in FC compared to the CC in a traditional rearing system.","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":"91 4","pages":"397-402"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0