Journal of Dairy Research最新文献

{"title":"Probiotic low-fat ice cream from goat milk: Characterization and resilience to simulated gastrointestinal conditions.","authors":"Imen Mahmoudi, Mouna Boulares, Olfa Ben Moussa, Moncef Chouaibi, Mnasser Hassouna","doi":"10.1017/S0022029925100915","DOIUrl":"https://doi.org/10.1017/S0022029925100915","url":null,"abstract":"<p><p>We aimed to develop a new ice cream made from goat milk inoculated with the probiotic bacteria <i>Limosilactobacillus fermentum</i> CABA16. The physicochemical characteristics, meltdown behaviour and sensory properties of ice cream produced with and without the probiotic bacteria were analysed. The ice cream with added <i>L. fermentum</i> was further evaluated for probiotic viability during frozen storage and simulated gastrointestinal conditions. Results showed that the addition of <i>L. fermentum</i> CABA16 decreased the pH to 6.25 (<i>P</i> < 0.05), but had no effect on physicochemical properties such as overrun and melting behaviour of ice cream samples. The viable probiotic count was 6.71 log CFU/g with a survival rate of 90%, which was registered after 120 days of frozen storage. Considering the probiotic cell viability during gastrointestinal conditions, exposure to bile and pancreatin for 6 hours resulted in a decline of 3.6 log CFU/g cycles in ice cream samples previously stored at -18 °C for 120 days. Overall, the goat milk ice cream inoculated with <i>L. fermentum</i> received good sensory scores, and satisfactory probiotic viability (6.7-7 log CFU/g) was maintained throughout the 120 days of frozen storage.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.2,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of flow cytometric tools to characterize milk somatic cells in water buffalo.","authors":"Maria Carmela Scatà, Francesco Grandoni, Giovanna De Matteis","doi":"10.1017/S0022029925000329","DOIUrl":"https://doi.org/10.1017/S0022029925000329","url":null,"abstract":"<p><p>The aim of this Research Communication was to develop new flow cytometric tools for the fine identification and characterization of milk somatic cells in water buffalo (<i>Bubalus bubalis</i>). Four multicolour panels of antibodies were designed to identify different subsets of live leukocytes and epithelial cells in bulk milk samples. Panel 1, including the CD18/CD172a/CD14/CD16 markers and Live/Dead vitality dye, allowed us to identify total lymphocytes, polymorphonuclear neutrophils (PMN) and monocyte/macrophage subsets. Panel 2 (CD18/CD4/CD8/δ chain/CD335 and Live/Dead dye) allowed us to identify T helper (CD4⁺), T cytotoxic (CD8⁺), γδ lymphocytes and NK cells. Panel 3 (CD18/CD79a/CD21 and Live/Dead dye) allowed us to identify total and CD21⁺ B lymphocytes. Finally, with Panel 4 (CD18/MHC-I/pan Cytokeratin and Live/Dead dye) the epithelial cells were distinguished from leukocytes. In conclusion, we propose a fine characterization of live milk somatic cell (live differential cell count (LDCC)) in buffalo species. In the future the determination of LDCC could used to identify new markers for detecting early inflammatory states of the mammary gland or for monitoring the technological properties of milks of different somatic cell composition.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-4"},"PeriodicalIF":1.2,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal control of the mammary blood-milk barrier and its role in establishing and maintaining milk production.","authors":"Kerst Stelwagen","doi":"10.1017/S0022029925000263","DOIUrl":"https://doi.org/10.1017/S0022029925000263","url":null,"abstract":"<p><p>The blood-milk barrier (BMB) forms at parturition when the gland switches form a non-lactating state to one of copious milk production and becomes leaky again when milk removal ceases and mammary involution is initiated. In this review the importance of the BMB in milk production and, in particular, its hormonal regulation is explored. Tight junctions (TJ) between adjacent mammary epithelial cells form a barrier to the two-directional paracellular movement of small molecules between the blood and milk and are responsible for establishing and maintaining the BMB. They form part of the cell's junctional complex and consist of transmembrane proteins that are linked to the mammary cell's cytoskeleton. This means that when, during lactation, TJ become \"leaky\" the resulting perturbation of the cytoskeleton interferes with the cell's secretory function. As such, TJ are involved in regulating and maintaining milk production. Mammary TJ are under hormonal control, with progesterone, glucocorticoids, prolactin, parathyroid hormone-related peptide (PTHrP) and serotonin (5-HT) being the key hormones. Progesterone prevents closure of TJ and the immediate prepartum drop in its concentration is a prerequisite for TJ closure. A simultaneous increase in the levels of glucocorticoids and prolactin is necessary for full TJ closure and initiation and maintenance of lactation. Both PTHrP and 5-HT are important hormones in maintaining extracellular calcium concentrations, a requirement for maintaining TJ integrity. Whereas PTHrP reduces TJ permeability, necessary for establishing and maintaining milk production, 5-HT has an opening effect on TJ. The latter may help speed up mammary involution and facilitate the movement of immune factors into the gland, preventing intramammary infections. In summary, mammary TJ make up the BMB and play a role in establishing and maintaining milk production and are under hormonal control, with progesterone, glucocorticoids, PTHrP and 5-HT being key regulatory hormones and prolactin likely playing a supporting role.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.2,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative thistle-curdled cheeses from the Mediterranean area: Nutritional evaluation of some relevant compounds.","authors":"Mena Ritota, Maria Gabriella Di Costanzo, Giorgia Rampanti, Lucia Aquilanti, Cindy María Bande De León, Luis Tejada, Akis Psomas, Bouthaina Al Mohandes Dridi, Pamela Manzi","doi":"10.1017/S0022029925100903","DOIUrl":"https://doi.org/10.1017/S0022029925100903","url":null,"abstract":"<p><p>Since ancient times, thistles have been used as clotting agents in the production of traditional cheeses, particularly in the Mediterranean area. In recent years, their use in cheesemaking has increased to satisfy the growing requests from vegetarian consumers. In this research paper, four different cheeses, typical of the Mediterranean area, were evaluated from a nutritional point of view: <i>Caciofiore</i> (from Italy) and <i>Torta del Casar</i> (from Spain), both typically produced using vegetable rennet, and <i>Queso de Murcia al vino</i> (from Spain) and <i>Feta</i> (from Greece), traditionally produced using animal rennet. All the cheeses were manufactured according to their traditional cheesemaking procedures and used as controls. Experimental cheeses were produced using aqueous extracts obtained from flowers of either spontaneous or cultivated thistles indigenous to the Mediterranean area (respectively <i>Onopordum tauricum</i> for Caciofiore, and <i>Cynara humilis</i> for Torta del Casar, Queso de Murcia al vino, and Feta). All cheeses were characterized for fat-soluble and mineral compounds to assess their nutritional adequacy according to the recommended daily intake of each evaluated nutrient. All the cheeses were found to be a good source of vitamin A, calcium and phosphorus, with an optimal Ca/P molar ratio, except for Feta. By consuming the recommended serving (50 g) of the studied cheeses, the salt and cholesterol intake is, on average, 16.4% and 15.9%, respectively of recommended intake. The use of aqueous thistle extracts in cheesemaking appears to have no effect on the nutritional quality of the studied cheeses.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.2,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of dairy production in selected African and Asian countries: challenges and opportunities for sustainability.","authors":"Habtamu Lemma Didanna, Abera Anja","doi":"10.1017/S0022029925100885","DOIUrl":"https://doi.org/10.1017/S0022029925100885","url":null,"abstract":"<p><p>There is increasing demand for milk and dairy products and an associated increase in milk production in Asia and Africa, making them important emerging dairy markets for the future. To the best of our knowledge, there has been little effort to comprehensively review literature on dairy production in these regions despite the changing situation, growth and challenges that require sustainable solutions. Thus, the objective of this review was to present an overview and evaluation of the dairy industry in selected countries in Eastern Africa and Asia using recent literature. The countries were selected based on the potential of dairy production in the respective regions. It focused on two types of countries: those in East Africa, which are at different stages of intensification regarding the global production issue, and those in Asia, which have large dairy industries. Based on this, Ethiopia, Kenya and Tanzania were selected from eastern Africa, while India, Pakistan and China were selected from Asia. The review revealed that dairy production in Eastern Africa predominantly relies on small-scale production systems. Factors such as inadequate feed, disease prevalence, poor access to breeding and formal/organized market pose significant challenges to this region's dairy industry. However, recent efforts have focused on improving productivity through technology adoption, livestock breeding programmes and market development initiatives. In contrast, Asia showcases a diverse range of dairy production systems. Countries like India are known for their large-scale dairy operations involving both indigenous and exotic dairy breeds. Additionally, cooperative models and public-private partnerships have contributed to the growth of the dairy sector in Asian countries. Nevertheless, challenges such as land/feed availability, environmental concern, and market competitiveness remain areas for improvement. While Eastern Africa aims to enhance small-scale farming systems through partly upgrading scale of production, innovation and market access, Asia seeks to bridge the gaps in productivity and sustainability.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.2,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bovine lactoferrin and lactoferrin-derived peptides on planktonic cells and abiotic surface biofilms of <i>Salmonella enterica</i>.","authors":"Uriel A Angulo-Zamudio, Adrian Canizalez-Roman, Hector Flores-Villaseñor, Jan G M Bolscher, Kamran Nazmi, Claudia Leon-Sicairos, Erika Acosta-Smith, Laura E Quintero-Martínez, Nidia León-Sicairos","doi":"10.1017/S0022029925100897","DOIUrl":"https://doi.org/10.1017/S0022029925100897","url":null,"abstract":"<p><p><i>Salmonella enterica</i> is a Gram-negative bacterium responsible approximately for 155,000 deaths annually. <i>S. enterica</i> is one of the most important foodborne pathogens, affecting mainly people in developed countries. The human immune system produces antibacterial peptides and proteins like lactoferrin (LF). This work addresses the hypothesis that bovine lactoferrin (bLF) and its derivative peptides bLactoferricin17-30, bD-Lactoferricin17-30, bLactoferrampin265-284, bD-Lactoferrampin265-284 and bLF-chimera have antimicrobial activity on planktonic cells and pre-formed biofilms of <i>S. enterica</i>. Planktonic <i>Salmonella enterica</i> ATCC 14028 were treated with bLF and bLF-peptides for two hours, and bacterial viability was determined by counting colony-forming units/ml. In addition, <i>S. enterica</i> biofilms were pre-formed or established on an abiotic surface, and viability or disruption was assessed in the presence of bLF and bLF-peptides by counting colony-forming units/ml or using the live/dead viability kit. We observed that bLF and bLF-peptides were bactericidal against planktonic <i>S. enterica</i>, killing more than 80% of cultures after two hours of treatment. The bactericidal effect was concentration and time-dependent. In addition, bLF, bLFampin165-284, and bLF-chimera showed an anti-biofilm effect against <i>Salmonella</i> biofilms pre-formed during 8 and 12 hours on the abiotic surface, disorganizing more than 50% of the biofilms after 4 or 6 hours of treatment. We conclude that bLF and its peptides show antimicrobial activity against planktonic cells and pre-formed biofilms of <i>S. enterica</i> on abiotic surfaces and could potentially be a therapeutic solution to combat <i>Salmonella</i> infections.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.2,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of lactoferrin supplementation in milk replacer on calf growth, feed efficiency and scouring incidence preweaning and postweaning.","authors":"Jennifer Felton, Ellan Dufour, Makaila Klejeski, Bruce Ziegler, David Ziegler, Mark Scott, Isaac Salfer","doi":"10.1017/S0022029925000251","DOIUrl":"https://doi.org/10.1017/S0022029925000251","url":null,"abstract":"<p><p>We investigated the hypothesis that supplementing milk replacer (MR) with exogenous lactoferrin (LF) would improve average daily gain (ADG) and feed efficiency and decrease scouring incidence in dairy calves. Lactoferrin is an antimicrobial and anti-inflammatory glycoprotein naturally found in bovine colostrum and milk that is low in MR. Previous studies suggest that supplementing LF to MR enhances ADG and feed efficiency while reducing disease occurrence in pre-weaning dairy calves. In our experiment, 103 Holstein heifer calves were randomly assigned to 1 of 4 treatments in a randomized complete block design from birth to d56 of age. Each calf received 340.1 g/d of 24% protein, 20% fat basal MR fed twice daily from d1 to 42 and once daily from d43 to 49, supplemented with 0 (L0), 1 (L1), 2 (L2) or 4 (L4) g/hd/d of LF treatment (45% purity). Calves were weaned at 49d of age. Body weight was measured at d1, 14, 28, 42, 49 and 56 of age. Faecal scores were measured weekly. Milk replacer and calf starter intake was measured daily and calculated biweekly. Data were analyzed using a linear mixed model with fixed effects of LF inclusion, and random effects of source herd and nursery room. In the first two weeks of life, ADG and gain-to-feed ratio (G:F) were numerically (non-statistically) increased in L4 tended compared with L1 and L2, but this effect was not maintained throughout the rest of the pre-weaning period or entire experiment. Average faecal score during the entire 56d experiment was greater in L2 compared with L0, L1 and L4, although faecal scores of all treatment groups were generally low. Under the conditions of the present study, LF supplementation at the inclusion levels provided showed minimal effects on feed intake, growth rate or calf health.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144816845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges for animal production in the changing world: A swan song.","authors":"Christopher H Knight","doi":"10.1017/S0022029925101295","DOIUrl":"https://doi.org/10.1017/S0022029925101295","url":null,"abstract":"","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-3"},"PeriodicalIF":1.2,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of feeding increasing levels of whole cottonseed on milk production, milk components and milk fatty acid profile in lactating dairy goats.","authors":"Tássia Martins, Maria Ferreira, Hemilly de Sá, Eduardo Duarte, Matheus Abreu, Débora Soares, Luciano de Lima, Iran Borges","doi":"10.1017/S0022029925000299","DOIUrl":"https://doi.org/10.1017/S0022029925000299","url":null,"abstract":"<p><p>We assessed the feeding behavior, intake, apparent total tract digestibility, blood parameters, milk yield, milk composition and milk fatty acid profile in dairy goats fed diets containing increasing levels of whole cottonseed (WCS), specifically a control diet with no WCS and diets containing 120, 150 and 180 g WCS/kg DM. Eight lactating dairy goats were distributed in a replicated 4 × 4 Latin square design with four 21-day periods. Rumination and total chewing time increased linearly with WCS inclusion levels. However, no effects on dry matter intake or digestibility were observed. Milk production was similar among treatments, but milk fat and lactose concentration increased linearly with WCS dietary levels, as did monounsaturated and polyunsaturated FA and conjugated linoleic acid concentration in milk fat. This study demonstrates that increasing the inclusion of WCS in the diets of dairy goats offers potential nutritional benefits without negatively impacting intake, digestibility or ruminal fermentation. Notably, WCS-enhanced diets led to improved milk fat composition, with increased concentrations of beneficial FA. These results support the use of WCS as an effective feed ingredient to enhance milk quality in dairy goats, leveraging both the high-energy content and fiber structure of WCS, which promotes rumen health and function without reducing DMI. Dairy goat producers can incorporate up to 180 g WCS/kg DM in diets to produce milk with a healthier fat profile, potentially enhancing its marketability.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.2,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-step Bayesian regression methods for genomic evaluation of milk yield of Murrah buffaloes.","authors":"Thalita Bianca Paiva, José Macedo, Jaliston Júlio Alves, Daniel Santos, Rusbel Raul Aspilcueta-Borquis, Humberto Tonhati, Francisco Araujo Neto","doi":"10.1017/S0022029925000317","DOIUrl":"https://doi.org/10.1017/S0022029925000317","url":null,"abstract":"<p><p>In this Research Communication we describe the application of single-step Bayesian regression (ssBR) models to predict milk yield of Murrah buffaloes. Milk production records of 2,026 cows in their first lactation were used. Using 270-day cumulative milk yield records as phenotype, genomic breeding values were predicted and their accuracies and dispersions were calculated by five methods: BayesA (ssBA), BayesB (ssBB), BayesC (ssBC); Bayesian Lasso (ssBL); and Bayesian ridge regression (ssBRR). For models based on mixture distributions (ssBB and ssBC), the proportions of markers having effect (π) were assumed as fixed, with respective values of 99% or 90%, or as unknown, where two approaches to estimate π were applied (ssBayesBπ and ssBayesCπ). The accuracy values found ranged from 0.550 (ssBBπ) to 0.584 (ssBCπ) and, the dispersion estimates ranged from 0.867 (ssBA) to 0.958 (ssBRR). The results indicated that Bayesian Lasso was the most suitable model for genetic evaluation of milk yield by buffaloes, considering accuracy and dispersion as criteria.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-3"},"PeriodicalIF":1.2,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}