Journal of Dairy Research最新文献

{"title":"Functional impact of c.V15M variation in LHβ gene on silent oestrus behaviour in river buffalo of Pakistan.","authors":"Asif Nadeem, Haroon Iqbal, Somia Shehzadi, Maryam Javed","doi":"10.1017/S0022029925000342","DOIUrl":"https://doi.org/10.1017/S0022029925000342","url":null,"abstract":"<p><p>Buffalo are shy breeders and poor fertility traits are a major hindrance in exploiting the production potential of the animal. This study hypothesizes that polymorphisms in the luteinizing hormone beta (LHβ) gene can affect oestrus behaviour in buffaloes. A total of 100 animals were screened by calculating the heat index (threshold-50) and animals were categorized into two groups (Group1 > 50, Group2 < 50). Animals were subjected to blood sampling, genomic DNA isolation, specific primer based polymerization and sequencing of amplicons. A total of six genomic variations were identified in the gene. c.V15M was a non synonymous mutation found in line with the Hardy Weinberg Equilibrium and was significantly associated with the trait. Functional impact of the variation was determined by three-dimensional structure of the protein. Effect of c.V15M on the functionality of the gene was evident and hypothesis was supported so this can potentially be used as a marker for the future development of superior animal breed or regulating the expression of the gene to get the optimal oestrus cyclicity in river buffalo of Pakistan.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of intramammary infection pathogens in dairy herds of the Northern Apennine mountains, Italy.","authors":"Alessandro Bellato, Stefania Bergagna, Martina Moriconi, Sarah Henry Whitaker, Federica Traverso, Alessandro Mannelli","doi":"10.1017/S0022029925000676","DOIUrl":"https://doi.org/10.1017/S0022029925000676","url":null,"abstract":"<p><p>This Research Communication presents a cross-sectional study to compare the prevalence of contagious, environmental, opportunistic and other intramammary infection pathogens in mountain dairy herds. In the Italian Apennine mountains, areas where dairy farming is thriving are interspersed among areas where only a few dairy herds remain. The disappearance of some dairy farms relates to the reduction of agricultural and veterinary services in a process that can jeopardize dairy herd profitability. Sixteen herds were screened for intramammary infection (IMI) pathogens. Antimicrobial susceptibility testing of mastitis pathogens was performed, and data on antimicrobial use was collected from the herd treatment registry. The prevalence of contagious IMI pathogens was significantly higher in areas where dairy farming is reducing, whereas environmental and opportunistic infections were more abundant in herds in thriving areas where farmers had a more consistent relationship with veterinarians. Antimicrobial resistance levels were low throughout all areas and did not relate to antimicrobial use, although it was significantly higher in areas where dairy herds were thriving.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding the residue from the extraction of cassava starch to dairy cows: Effects on nutrient digestibility and performance.","authors":"Cleo Rossi Jarvoski, Fabio Dos Santos, Maria Fischer, Caroline Hoscheid Werle, Deise Castagnara, Rodrigo Dos Reis Tinini, Geraldo Dos Santos, Maximiliane Alavarse Zambom","doi":"10.1017/S0022029925000287","DOIUrl":"https://doi.org/10.1017/S0022029925000287","url":null,"abstract":"<p><p>The effect of different levels of residue from the extraction of cassava starch (REAM) in the diet of multiparous lactating Holstein cows was investigated. The experiment was carried out at the Universidade Estadual do Oeste do Paraná. Five multiparous Holstein cows, with mean milk production of 30.65 ± 1.17 kg/day were distributed into five treatments, using Latin squares (5 × 5). Treatments consisted of inclusion (0, 8, 16, 24 and 32% dry matter basis) of REAM in the diet. Intake, digestibility, milk production and composition as well as blood parameters were measured. Except for ether extract and total digestible nutrients, the intake variables did not differ significantly between treatments. Except for the digestibility of crude protein, all digestibility values decreased progressively with inclusion levels of REAM. The inclusion of REAM in feeding of lactating Holstein cows up to 32% of the diet reduced milk production by 15%, however, it did not alter milk production efficiency, fat, total solids and blood parameters. We conclude that up to 16% of REAM can be included in the diet; however, higher levels may lead to a significant fall in productivity.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of body weight of growing dairy buffaloes from body volume.","authors":"Marco Ramírez-Bautista, Alvar Cruz-Tamayo, Jorge Canul-Solís, Luis Castillo-Sánchez, Tairon Dias-Silva, Antonio Gurgel, Daniel Mota-Rojas, Alfonso Chay-Canul","doi":"10.1017/S0022029925000305","DOIUrl":"https://doi.org/10.1017/S0022029925000305","url":null,"abstract":"<p><p>Among body measurements, body weight (BW) is one of the most important within the buffalo production system, due to its association with economic characteristics. In previous research, we have shown that body volume (BV) is an effective predictor of BW in lactating adult water buffalo. As there are no equations to predict BW through BV for growing dairy buffaloes (young animals), we hypothesized that equations should be developed to meet this need. BW, body length (BL) and heart girth (HG) data were collected in 160 growing dairy buffaloes raised in commercial farms in southern Mexico, with body volume (BV) then estimated from BL and HG. The ratio between BV and BW was determined by linear, quadratic and allometric equations. The goodness-of-fit of the regression models was evaluated using the Akaike information criterion (AIC), the Bayesian information criterion (BIC), the coefficient of determination (R²), the mean square error (MSE) and the root MSE (RMSE). After this, the <i>k</i>-folds cross-validation was performed to indicate a better fit. Our results showed that the growing dairy buffaloes presented a BW of 256.6 ± 96.82 kg and a BV of 155.3 ± 74.87 dm3. High and positive correlation were observed among all variables studied. All parameters (R2, MSE, RMSE, AIC and BIC) used to evaluate the regression equations showed that the quadratic regression model was more effective than the linear and allometric models for estimating BW using BV. The criteria for evaluating and validating models showed that the quadratic model presented a better predictive performance. Based on these findings, we conclude that body volume data to estimate body weight of growing dairy buffaloes were best fitted using the quadratic regression model.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-4"},"PeriodicalIF":1.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of stevia (<i>stevia rebaudiana</i>) and hemp by-products (<i>cannabis sativa</i>) on milk yield, fatty acid profile, and oxidative stability in dairy cows.","authors":"Konstantinos Kalaitsidis, Zoi Parissi, Alexandros Theodoridis, Soumela Savvidou, Basiliki Kotsampasi, Konstantina Vasilopoulou, Stella Dokou, Paraskevi Chantzi, Eleni Tsaliki, Diamanto Lazari, Georgios E Valergakis, Ilias Giannenas","doi":"10.1017/S0022029925000275","DOIUrl":"https://doi.org/10.1017/S0022029925000275","url":null,"abstract":"<p><p>This experiment assessed the effects of adding hemp (seeds or hay) and stevia by-products to dairy cow diets on milk yield and on the fatty acid profile and oxidative stability of the milk. Additional analyses included composition, total phenolic content and total antioxidant capacity of milk samples as well as blood serum parameters. Thirty-five Holstein dairy cows were involved for 60 days, divided into five groups: control, hemp seed, hemp hay, stevia and a combination of hemp seeds and stevia leaves. No significant changes were observed in milk yield or composition. While monounsaturated fatty acid (MUFA) content did not differ significantly between control and experimental diets, milk from cows fed hemp seeds had higher MUFA compared to those fed hemp hay. Further research is recommended to determine the optimal proportion of these by-products in cow diets.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of different physiological conditions of riverine buffaloes (<i>bubalus bubalis</i>) based on their vocal cues through machine learning algorithms and a conventional statistical model.","authors":"Indu Devi, Naresh Kumar Dahiya, A P Ruhil, Yajuvendra Singh, Divyanshu Singh Tomar","doi":"10.1017/S0022029925100976","DOIUrl":"https://doi.org/10.1017/S0022029925100976","url":null,"abstract":"<p><p>To understand the requirements of animals their calls can be analysed. This potentially enables specific and more precise individual care under different emotional and physiological conditions. This study was conducted to identify three different conditions (oestrus, delayed milking and isolation) of buffaloes based on vocalization patterns. A total of 600 acoustic samples of buffaloes for each condition were collected under different conditions consisting of 300 records for confirming and 300 for non-confirming of a particular condition. Important acoustic features like amplitude (P), total energy (P²s), pitch (Hz), intensity (dB), formants (Hz), number of pulses, number of periods, mean period (sec) and unvoiced frames (%) were extracted using the MFCC (mel frequency cepstrum coefficients) technique. Algorithms (model) were trained by partitioning the acoustic data into training and validation sets to develop predictive models. Three different ratios were assessed: 60%-40%, 70%-30% and 80%-20%. Decision tree models were optimized based on decision and average square error (probability) options and other parameters were set to default values of the software package to deveop the best model. The performance of algorithms was evaluated on the parameter accuracy rate. Decision tree models predicted the physiological conditions oestrus, isolation and delayed milking with an accuracy of 66.1, 84.3 and 71.3%, respectively, while the logistic regression model predicted with an accuracy rate of 59.5, 71.1 and 65.7%, respectively, and the artificial neural network (ANN) model predicted these three conditions with 77.7, 85.2 and 79.4% accuracy, respectively. The ANN model was found to be best on the basis of minimum misclassification rate (on 80%-20% portioning). However, decision tree algorithms also provided the additional information that intensity (maximum), amplitude (minimum) and formant (F1) are the most important features of vocal signals to identify physiological conditions like oestrus, isolation and delayed milking respectively in dairy buffalo.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large scale subclinical ketosis risk assessment in dairy herds using predicted milk acetone and β-hydroxybutyrate via MIR technology.","authors":"Fabio Abeni, Andrea Ferla, Riccardo Negrini, Andrea Galli","doi":"10.1017/S0022029925000366","DOIUrl":"https://doi.org/10.1017/S0022029925000366","url":null,"abstract":"<p><p>Sub-clinical ketosis (SCK) significantly affects post-partum dairy cow performance and welfare. A total of 11,327,959 test-day (TD) records over two years on 1.76 million Holstein cow lactations and 2840 farms were processed to ascertain thresholds for milk acetone (mACE) and β-hydroxybutyrate (mBHB) as indicators of SCK on the basis of a significant milk yield loss at the TD. The set thresholds for mACE and mBHB were 0.10 mmol/L and 0.14 mmol/L, respectively. The prevalence of SCK in the population during the first 60 days in milk (DIM) was estimated based on herd size and milk yields, utilizing one or both of these metabolites surpassing their respective thresholds. Analyzing both mACE and mBHB together revealed a higher occurrence of SCK in small herds (fewer than 100 cows) and a lower occurrence in the two most productive milk categories. The prevalence had an inverse relationship with the daily milk yield at 60 DIM, indicating a surprisingly high frequency of low-productivity herds in the risk classes exceeding 30%. These results suggest that assessing SCK prevalence through the combined evaluation of mACE and mBHB is a more effective approach than using the milk fat to protein ratio, especially when considering different herd sizes and daily milk yield at 60 DIM.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Somatic cell count and presence of microbial pathogens in milk of goats in Slovakia.","authors":"Barbora Gancárová, Kristína Tvarožková, Marta Oravcová, Michal Uhrinčať, Lucia Mačuhová, Dušan Vašíček, Ľudovít Černek, Vladimír Tančin","doi":"10.1017/S0022029925000354","DOIUrl":"https://doi.org/10.1017/S0022029925000354","url":null,"abstract":"<p><p>The objectives of the study were to determine somatic cell count (SCC) and evaluate the presence of pathogens (IMI - intramammary infection) in late lactation (LL), followed by the start (colostrum, CL) and approximate peak (established lactation, EL) of the next lactation, as well as to assess the possible transmission of IMI from lactation to lactation. The study was performed on a dairy farm in northern Slovakia. A total of 489 half udder milk samples (242, 80 and 167 in LL, CL and EL, respectively) were collected. Pathogens were identified using MALDI-TOF MS and PathoProof (the latter only in LL). SCC was determined only in LL and EL. Samples were divided according to SCC in four groups from lowest (SCC1 < 500 × 10³ cells mL^-1) to highest (SCC4 ≥ 2000 × 10³ cells mL^-1). SCC was higher in LL than in EL. The prevalence of pathogens identified using MALDI-TOF MS was 16.5, 38.8 and 12.6% in LL, CL and EL, respectively. Non-<i>aureus</i> staphylococci and mammaliicocci (NASM) were the most common isolated pathogens in goat milk and colostrum. <i>Staphylococcus (S.) caprae</i> and <i>S. epidermidis</i> species tended to cause persistent IMI in the next lactation. The identification of pathogens using PathoProof was higher than with MALDI-TOF MS. Of all the pathogens (n = 262) identified using PathoProof, the most common were <i>Staphylococcus</i> spp. (86.7%) of which 65.8% exhibited the <i>β</i>-lactamase gene. Additionally, <i>Escherichia coli</i> (4.2%), <i>S. aureus</i> (2.7%), <i>Enterococcus</i> spp. (2.3%), <i>Streptococcus uberis</i> (1.9%), <i>Mycoplasma</i> spp., <i>Protetheca</i> spp. (0.8% each), <i>Arconabacterium pyogenes</i>/<i>Peptoniphilus indolicus</i> and yeast (0.4% each) were also detected using PathoProof. Better identification of pathogen presence in samples with high SCC could contribute to the discussion about SCC as an indicator of subclinical mastitis in goats.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-3"},"PeriodicalIF":1.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of innate immunity and haematological changes in Holstein calves during the gradual weaning process.","authors":"Karen Nascimento Silva, Karina Medici Madureira, Camila Cecília Martin, Daniela Irlanda Castro Tardón, Bianca Paola Santarosa, David John Hurley, Viviani Gomes","doi":"10.1017/S0022029925000330","DOIUrl":"https://doi.org/10.1017/S0022029925000330","url":null,"abstract":"<p><p>The weaning process negatively affects the haematological parameters and innate immune response of dairy calves, even when managed under an intensive milk program. Here we describe haematological and innate immunity changes in 47 Holstein calves aged 69-85 days subjected to a gradual weaning process. Blood samples were collected at six (D-6), four (D-4), and two (D-2) days before and on the weaning day (D0) for the phagocytosis assay and to measure the production of reactive oxygen species (ROS) after stimulation with <i>Staphylococcus aureus, Escherichia coli</i>, and <i>Mannhemia haemolytica</i>, in addition to total protein (TP), haptoglobin (Hp), and iron concentration. The highest mean neutrophil number was recorded at D-2. The absolute number of monocytes was initially high on D-6 and D-4 but declined as the calf progressed to weaning. The number of basophils decreased rapidly, reaching a low value on D-4, and remained low for the remainder of the study period. The TP, Hp, and Fe concentrations decreased. Overall, polymorphonuclear leukocyte phagocytosis activity induced by <i>S. aureus</i> and <i>E. coli</i> decreased from D-6 to D-2, indicating persistence of the low phagocytosis rate for <i>S. aureus</i>. ROS production was constant for all bacterial stimulations from D-6 to D-2, followed by an increase on D0. Phagocytosis and ROS production indicate that the weaning process dampens the innate immune response relative to exposure to these common pathogenic bacteria in dairy calves. Phagocytosis and the corresponding indicators of intracellular killing activities (ROS production and myeloperoxidase index) represent the most accepted core mechanisms for the early elimination of pathogenic microorganisms in calves. Despite a slow gradual weaning management system, the study concluded that intensive milk production programs contribute to innate immune response suppression during weaning.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vechur cow milk yoghurt: response surface methodology-based process optimization and storage studies.","authors":"Ammiti Murali Krishna, Ligimol James, Athrayil Kalathil Beena, Sujjalur Nagesha Rao Rajakumar, Kollannur Aippunny Mercey","doi":"10.1017/S0022029924000645","DOIUrl":"https://doi.org/10.1017/S0022029924000645","url":null,"abstract":"<p><p>In this research article, response surface methodology (RSM) based optimization of three production parameters namely temperature, time and amount of starter culture of Vechur cow milk yoghurt (VCMY) on the basis of sensory evaluation responses comparing cross-bred cow milk yoghurt (CCMY) as the control is reported. The optimized values of production parameters were 2.15 per cent rate of inoculation, 42°C incubation temperature and 4 h incubation period. The optimized product exhibited significantly lower syneresis, <i>a</i>*, <i>b</i>* values and higher <i>L</i>* values than CCMY. Physico-chemical, microbiological, textural and sensory properties of both VCMY and CCMY during room temperature and refrigerated storage were assessed daily until the onset of spoilage (room temperature) or at five day intervals over a period of 15 d (refrigerated). Both room temperature stored products were graded undesirable by the sensory panel upon one day of storage. Significant reduction was observed in the fat, SNF, total solids, protein and pH content and all the tested colour parameters of the optimized product during refrigerated storage. Total viable counts as well as yeast and mould counts and lactic acid bacteria counts of both VCMY and CCMY progressively increased over the 15 d of storage. Significant reductions were observed in the flavour (<i>P < 0.01</i>), body and texture, colour and appearance and overall acceptability (<i>P < 0.05</i>) scores of both the samples over a period of 15 d. During storage, hardness and adhesiveness values showed an increasing trend whereas the cohesiveness showed a decreasing trend. Storage studies revealed significant differences in the acidity, pH, syneresis, tyrosine value, colour parameters and sensorial attributes of both the yoghurt samples. During the 15 d refrigerated storage period, the VCMY exhibited superior technological attributes to CCMY in terms of lower syneresis %, acidity, microbial population, firmer and less cohesive texture, better flavour, colour and appearance scores. Being the first comprehensive study on the utilization of Vechur cow milk for the preparation of yoghurt, the data generated in the current study would provide a solid base for the exploration of fermentation as a means of value addition of milk of this very rare indigenous cattle breed.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}