Journal of Drug Delivery Science and Technology最新文献

{"title":"Machine learning assisted chitin Extraction: Source and yield prediction from crustacean biomass using deep eutectic solvents","authors":"Sasireka Rajendran ,&nbsp;Vinoth Rathinam ,&nbsp;Valarmathi Krishnasamy ,&nbsp;Kaushik Pal","doi":"10.1016/j.jddst.2025.107548","DOIUrl":"10.1016/j.jddst.2025.107548","url":null,"abstract":"<div><div>Chitin is the primary polymer after cellulose found in the crustacean shells, insects, and fungi. In recent years, extracting chitin from crustacean shells has garnered attention as a means to convert waste into valuable resources, given its promising applications in various fields. While several methods have been employed to recover chitin, emerging green solvents such as deep eutectic solvents (DES) are increasingly favored to address the challenges posed by traditional chemical, enzymatic, and biological approaches. Chitin extraction efficiency is governed by multiple interdependent factors with complex, non-linear interactions, making conventional trial-and-error optimization both time-consuming and inefficient. These challenges highlight the need for advanced approaches, such as machine learning, to accurately model and optimize the process. Recognized for its reliability and adaptability, machine learning offers an effective approach to modeling the complex, non-linear interactions among process variables in chitin extraction, thereby enabling precise yield prediction and process optimization. The present work proposes a machine learning approach designed to accurately estimate chitin yield and optimize the key parameters governing its extraction. Experimental datasets from chitin extraction trials were used to train and evaluate multiple ML algorithms. Key process variables were treated as input features, with chitin yield as the output target. Among the models tested, XGBoost and Decision Tree achieved the highest predictive accuracy (R² = 0.99) with minimal root mean square error (RMSE) and mean absolute error (MAE) values. Model-driven optimization was then employed to identify the most favorable combination of process variables. Experimental extractions conducted under the predicted optimal conditions produced chitin yields closely matching the model forecasts, confirming high prediction accuracy. This research demonstrates that machine learning can serve as a cost-effective, efficient method for predicting chitin yield and paves the way for addressing complex issues involving large datasets. Further, the method offers a scalable strategy for enhancing biopolymer recovery and overcoming the multifaceted challenges posed by extensive datasets in sustainable materials research.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107548"},"PeriodicalIF":4.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized tolterodine tartrate loaded SNEDDS for enhanced oral bioavailability: In vitro and in vivo studies","authors":"Afzal Hussain ,&nbsp;Mohhammad Ramzan ,&nbsp;Danishuddin ,&nbsp;Mohd Usman Mohd Siddique ,&nbsp;Mohammad A. Altamimi","doi":"10.1016/j.jddst.2025.107554","DOIUrl":"10.1016/j.jddst.2025.107554","url":null,"abstract":"<div><div>The study investigated tolterodine tartrate (TOT)-loaded optimized SNEDDS for improved stability, facilitated absorption, and high oral bioavailability (BA). The QbD-approach optimized the robust composition and identified the impact of critical factors (olive oil = X₁ and cremophor EL = X₂) on the set responses (Y₁ = size, Y₂ = %Transmittance, and Y₃ = % entrapment efficiency). Thermodynamic stability testing was conducted under stressed conditions. The optimized SNE-TOT2 was evaluated for the formulation characteristics (size distribution, shape, and zeta potential), followed by comparative <em>in vitro</em> drug release and <em>ex vivo</em> intestinal permeation studies. <em>In vitro</em> hemolysis study surrogated acute preliminary toxicity study. Oral pharmacokinetics and hepatotoxicity studies were performed in rats under fast condition. The thermodynamically stable SNE-TOT2 (desirability ∼ 0.97) possessed desired formulations attributes (small size, 159 nm; low polydispersity index, 0.235; high zeta potential, −16.54 mV; high %EE, 81 %, and high %transmittance) and efficient self-emulsification performance. The impact of X₁ and X₂ on Y₁-Y₃ was quadratic, indicating the need for optimal selection of these factors. SNE-TOT2 showed anomalous (non-Fickian) drug release behavior (&gt; 80 % within 15 min) and as compared to SUS-TOT. SNE-TOT2 exhibited the highest intestinal permeation (219.9 ± 27.21 μg/cm²), suggesting superior absorption compared with SUS-TOT (59.2 ± 13.9 μg/cm²) and marketed (MKT-TOT) (128.2 ± 23.18 μg/cm²). Moreover, SNE-TOT2 improved pharmacokinetic parameters and reduced hepatic toxicity, compared to SUS-TOT and MKT-TOT. Thus, SNE-TOT2 can be a promising and safe oral product with improved oral BA.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107554"},"PeriodicalIF":4.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergetic effect of a laser-activated nanocomposite for GBM therapy","authors":"Setareh Ebrahimnasab ,&nbsp;Parviz Parvin ,&nbsp;Fatemeh Ramezani ,&nbsp;Ali Bavali ,&nbsp;Mahdi Ebrahimi","doi":"10.1016/j.jddst.2025.107547","DOIUrl":"10.1016/j.jddst.2025.107547","url":null,"abstract":"<div><div>Glioblastoma (GBM) is regarded as one of the most lethal human cancers. Despite recent progress in cancer therapy, it has remained an incurable disease. Thus, novel approaches are welcome to envision new protocols that improve current treatments to prolong patients' lives. Here, a novel nano-drug system is developed alongside selective chemotherapy accompanied by photodynamic therapy (PDT). The proposed nanocomposite (NCP) is composed of a targeting agent (fluoxetine), a chemotherapeutic drug (temozolomide), a photosensitizer (methylene blue), and zinc oxide nanoparticles (ZnO NPs). The system relies on ZnO NPs as nanocarriers that gradually release temozolomide (TMZ) in PBS at a pH of 7.4. The characterization of the synthesized NCP was carried out using manifold techniques (e.g., transmission electron microscopy (TEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDS)). Moreover, the NCP cytotoxicity is assessed by the MTT method, and the cellular uptake efficacy is analyzed using flow cytometry. The release amount is measured to be 15 % at 120 h to mitigate the intake of higher doses. The significant PDT parameters have been systematically determined, including the extinction coefficient (&amp;epsi) through modified Beer-Lambert (MBL), quantum yield (<em>η</em>_f), as well as the content of reactive oxygen species (ROS. Although the NCP's quantum yield (<em>η</em>_f = 0.24) is reduced by half against that of the MB (<em>η</em>_f = 0.52), the parameter of ε notably elevates (from 0.08 to 3.21 ml mg⁻¹ cm⁻¹). This causes a large absorbance at the laser line, resulting in the pronounced ROS content. The collaborative impact of NCP under laser exposure is also investigated through drug release assessment according to photo-bio-modulation (PBM), compared to the absence of laser irradiation. Laser stimulation gives rise to the prompt drug release within a cancerous pH environment of 6.9 (87 % during 40 min), attesting to the synergistic effect of coherent light on NCP. Hence, the systematic measurements reveal that NCP is a ROS-responsive composite for satisfactory drug delivery. The results further validate the cytotoxic effects of the NCP combined with laser treatment on C6 glioma cells, as demonstrated by the MTT assay. This innovative approach holds promise for improving therapeutic outcomes in glioblastoma treatment.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107547"},"PeriodicalIF":4.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Octaarginine nanocomplex encapsulated with hyaluronic acid-modified CuS for enhanced transdermal delivery of insulin","authors":"Xueling Long,&nbsp;Siyi Li,&nbsp;Yuxiang Ma,&nbsp;Yuting Cao,&nbsp;Shaoping Sun","doi":"10.1016/j.jddst.2025.107550","DOIUrl":"10.1016/j.jddst.2025.107550","url":null,"abstract":"<div><div>Transdermal delivery is considered as a promising convenient and painless approach for the delivery of insulin. In this study, an amphiphilic peptide derivative of oleic acid modified octaarginine (R8-OA) and a photothermal nanomaterial of hyaluronic acid modified CuS (CuS-HA) were successfully synthesized. Using both materials as carriers, CuS-HA enveloped R8-OA@insulin nanocomplexes (HERNCs) were prepared. <em>In vitro</em> studies demonstrated that R8-OA could chemically enhance the penetration of insulin through the stratum corneum. <em>In vivo</em> laser activation studies suggested that CuS-HA in HERNCs could ablate the stratum corneum, thus facilitating the insulin penetration through physical enhancement. The hypoglycemic effect study revealed that blood glucose level in diabetic mice decreased to 42.3 % within 4 h, and the value was maintained at 75.1 % after 24 h. These results underscored the potential of the enveloped nanocomplexes for enhanced transdermal delivery of insulin.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107550"},"PeriodicalIF":4.9,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and optimization of solvent-assisted gradient active loading technique (SGALT) to improve the encapsulation efficiency and ulcerative colitis efficacy of liposomal andrographolide derivatives","authors":"Ning Dong ,&nbsp;Guo Chen ,&nbsp;Junqi Liu ,&nbsp;Boyuan Liu ,&nbsp;Yun Zou ,&nbsp;Yingchao Zhang ,&nbsp;Yu Zhang ,&nbsp;Tian Yin ,&nbsp;Haibing He ,&nbsp;Jingxin Gou ,&nbsp;Yanjiao Wang ,&nbsp;Xing Tang","doi":"10.1016/j.jddst.2025.107549","DOIUrl":"10.1016/j.jddst.2025.107549","url":null,"abstract":"<div><div>Andrographolide and its derivatives have demonstrated significant anti-inflammatory properties, making them potential candidates for treating ulcerative colitis (UC). However, the clinical application of andrographolide injection via intravenous injection is often limited due to poor water solubility, severe adverse reactions, and poor targeting efficacy. This study developed a solvent-assisted gradient activity loading technique (SGALT) to improve the clinical application of dehydrated andrographolide succinate sesquiterpenes (DAS). DAS formed insoluble salt complexes in the interior of liposomes. The \"lock-in\" loading strategy exhibited a high encapsulation efficiency (89.78 ± 2.81 %) and sufficient DAS retention ability. There was 84.13 % of liposomal DAS that remained stable in plasma for 24 h, and the drug release was accelerated in the acidic environment of colitis. Moreover, the accumulation of DAS liposomes (DAS-Lip) was 3.3 times greater than that of free drugs in 24 h in colitis mice but not in normal mice due to extravasation through leaky vasculature and subsequent inflammatory cell-mediated sequestration (ELVIS) effect. Pharmacodynamic studies further demonstrated that DAS-Lip effectively improved anti-colitis efficacy in mice with higher disease activity index (DAI), lower inflammatory cytokine levels, and less toxicity. In conclusion, SGALT-mediated intra-liposomal drug locking facilitates the drugability of DAS and will provide a new idea for the treatment of UC.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107549"},"PeriodicalIF":4.9,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonic nanoengineering of biocompatible lignin-ergothioneine hybrids for targeted membrane disruption and sustained oral eradication of Helicobacter pylori","authors":"Mohamed Sharaf ,&nbsp;Tehsin Ullah Khan ,&nbsp;Yu Yu ,&nbsp;Busati Ahmed ,&nbsp;Zhe Chi ,&nbsp;Chen-Guang Liu","doi":"10.1016/j.jddst.2025.107545","DOIUrl":"10.1016/j.jddst.2025.107545","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> (<em>H. pylori</em>) is a gram-negative, spiral-shaped bacterium that infects approximately 4.4 billion people globally. In this study, a novel method combining high-intensity ultrasonic solvent treatment and homogenization was employed to functionalize and nano-transform lignin nanoparticles (LigNPs) for grafting ergothioneine (EGT), a natural hydrophobic antioxidant, onto LigNPs (EGT@LigNPs) to target the <em>H. pylori</em> cell membrane. Acetone, with its strong hydrogen bonding capacity, was found to aggregate lignin of various molecular weights and increase the concentration of phenolic hydroxyl groups. Transmission electron microscopy (TEM), dynamic light scattering (DLS), UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) revealed that LigNPs with a regular spherical shape and uniform size 86.5 ± 0.41 to 354.6 ± 3.44 nm could be produced by adjusting the water/solvent ratio and stirring rate during the antisolvent process. EGT@LigNPs demonstrated sustained drug release over 48 h and exhibited superior antioxidant and anti-<em>H. pylori</em> activities compared to unloaded LigNPs and pure EGT. Additionally, EGT@LigNPs showed excellent biocompatibility with normal a fibroblast cell line (L929), enhancing metabolic activity and intracellular protein release in <em>H. pylori</em>. Bioimaging studies using TEM, scanning electron microscopy (SEM), and confocal laser scanning microscopy (CLSM) indicated that EGT@LigNPs adhered to the bacterial surface and destabilized and disrupted the cell membranes. These findings suggest that EGT@LigNPs hold novel a non-toxic oral drug delivery system for treating gastric infections caused by <em>H. pylori</em>.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107545"},"PeriodicalIF":4.9,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of porous TC4 bone scaffold VEGF-Si/HA-TiO2 nanotube composite coating: crystal structure improvement and bio sustained release promoting angiogenesis and osteogenic regeneration","authors":"Yueheng Lei ,&nbsp;Jizhe Hai ,&nbsp;Xiangzhe Zhang ,&nbsp;Chunlong Shan ,&nbsp;Lei Jing ,&nbsp;Haijie Li ,&nbsp;Xuehai Ma","doi":"10.1016/j.jddst.2025.107540","DOIUrl":"10.1016/j.jddst.2025.107540","url":null,"abstract":"<div><div>Titanium-based porous implants achieve personalized structural matching and provide strong load-bearing capacity, which has led to their widespread application in bone repair. Epidemiological studies report that 5–10 % of orthopedic and dental implants fail within 10–15 years after surgery, mainly due to aseptic loosening caused by poor osteogenesis and vascularization. To address this issue, this study proposes a strategy for preparing functionalized coatings on the surface of titanium-based scaffolds to promote the dual regeneration of vascular and bone tissues. By adjusting the composition of different electrolytes, a silicon-doped hydroxyapatite (Si/HA) coating was successfully prepared on titanium dioxide nanotubes (TNTs), which subsequently served as a platform for VEGF loading. The study showed that he TiO₂ nanotubes formed on the porous micro-curved surface exhibited an average diameter of 180 nm and a length of 7.56 μm. By adding sodium silicate to the electrolyte, compared to pure HA crystals, the Si/HA crystals transformed from an irregular morphology to a columnar and vertically aligned crystal structure with respect to the substrate, which enhanced the mechanical interlocking behavior of the composite coating. Additionally, the increased surface charge of the Si/HA coating markedly enhanced its electrostatic adsorption capacity for VEGF, enabling controlled release rates. After 28 days, VEGF release followed a sustained-release profile, reaching 1.22 μg/ml. Cellular experiments confirmed that the synergistic release of VEGF and Si markedly promoted the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVEC). ALP、OCN and Runx2 activity analysis indicated that the bioactivity of the VEGF-Si/HA-TNTs coating was significantly higher than that of the other two groups on days 7 and 14. This study provides new insights into the functionalization of titanium-based implant coatings, highlighting their potential to improve clinical outcomes in bone repair, particularly through enhanced angiogenesis and osteogenesis.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107540"},"PeriodicalIF":4.9,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-dimensional metal-organic framework loaded zinc oxide nanocomposites for near-infrared light response synergistic antibacterial and accelerate wound healing","authors":"Xinlong Wang ,&nbsp;Yawen Zhang ,&nbsp;Haitao Zhu,&nbsp;Zhaofei Yang,&nbsp;Bing Hu,&nbsp;Yue Yuan","doi":"10.1016/j.jddst.2025.107539","DOIUrl":"10.1016/j.jddst.2025.107539","url":null,"abstract":"<div><div>Human health is seriously threatened by bacterial infections that are resistant to drugs. Therefore, there is an increasing demand for non-antibiotic methods to surmount the drug resistance of bacterial infections. As an attractive antibacterial strategy, mild photothermal therapy (PTT) shows great application potential because of its capacity to avoid medication resistance and outstanding biocompatibility. However, bacteria's resistance to heat limits its effectiveness. In this paper, a nanocomposite ZnO@Zr-Fc-MOF was established by growing ZnO nanoparticles (NPs) on Zr-Fc-MOF nanosheet surfaces. The nanocomposite provided controllable photothermal therapy of Zr-Fc-MOF nanosheets and the inherent bacteriostatic characteristics of ZnO NPs, achieving synergistic effects to eliminate drug-resistant bacteria and promote wound healing. Encouragingly, MTT and <em>in vitro</em> bacteriostatic results showed that the combination of PTT and ZnO NPs achieved 99.5 % bacteriostatic rate without killing normal cells. The results of animal experiments showed that ZnO@Zr-Fc-MOF can effectively cure wounds infected by methicillin-resistant <em>S. aureus</em>. Generally speaking, the prepared ZnO@Zr-Fc-MOF was a promising treatment scheme, which can effectively inhibit bacterial growth, promote wound healing and have good biocompatibility.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107539"},"PeriodicalIF":4.9,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-206-loaded albumin nanoparticles suppress ovarian cancer cells by inhibiting c-met oncogenic pathway","authors":"Sajjad Masoumi ,&nbsp;Seyed Ahmad Aleyasin ,&nbsp;Shahab Faghihi","doi":"10.1016/j.jddst.2025.107546","DOIUrl":"10.1016/j.jddst.2025.107546","url":null,"abstract":"<div><div>Ovarian cancer is one of the most lethal gynecological malignancies, often diagnosed at advanced stages with poor survival outcomes. Despite improvements in surgery and chemotherapy, resistance to conventional therapies remains a major obstacle, underscoring the urgent need for novel and biocompatible therapeutic strategies. MicroRNA-206 (miR-206) functions as a potent tumor suppressor by targeting the c-Met/PI3K/AKT/mTOR signaling pathway; however, its therapeutic application is limited by instability, enzymatic degradation, and inefficient cellular uptake. We therefore hypothesized that bovine serum albumin nanoparticles (BSA-NPs) could serve as an effective and safe carrier for miR-206, enhancing its intracellular delivery and restoring tumor-suppressive activity in ovarian cancer cells. Consistent with this rationale, the nanocarriers exhibited favorable morphology, biocompatibility, and efficient cellular uptake, leading to elevated intracellular miR-206 levels. Functional assays demonstrated that BSA-NPs loaded with miR-206 inhibited proliferation, migration, and invasion while inducing apoptosis in SKOV-3 cells. Gene and protein analyses further confirmed suppression of c-Met/AKT/mTOR signaling, downregulation of oncogenic and anti-apoptotic markers, and restoration of tumor-suppressive regulators. These findings highlight BSA-NPs as a promising preclinical platform for miR-206 delivery, capable of reactivating tumor-suppressive pathways in vitro, and provide a strong rationale for further validation across additional models to overcome key barriers in the clinical translation of miRNA-based therapeutics for ovarian cancer.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107546"},"PeriodicalIF":4.9,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart dressings accelerating wound healing with tranexamic acid-infused aligned electrospun nanofibers: In vitro and In vivo assessments","authors":"Abdullah M.M. Elbadry ,&nbsp;Eman Gomaa ,&nbsp;Mennatullah M. Faisal ,&nbsp;Elbadawy A. Kamoun ,&nbsp;Samar A. Salim","doi":"10.1016/j.jddst.2025.107542","DOIUrl":"10.1016/j.jddst.2025.107542","url":null,"abstract":"<div><div>The development of advanced wound dressings capable of accelerating healing and achieving effective hemostasis remains a critical challenge in managing traumatic and surgical wounds. This study reports the fabrication and comprehensive evaluation of a novel multilayered, sandwich-structured nanofiber scaffold composed of Tranexamic acid (TXA), chitosan (CS), polyvinyl alcohol (PVA), L-arginine, and polylactic acid (PLA) for biodegradable wound dressing applications. Using sequential electrospinning, a four-layered scaffold was developed as a smart wound dressing, comprising an immediate-release TXA-CS-PVA layer for rapid clotting, a hydrophobic PLA barrier layer for protection, a sustained-release L-arginine-PVA layer to promote tissue regeneration, and a final PLA protective layer to enhance durability. The morphology and fiber alignment were optimized by employing both flat plate and rotating disc collectors to achieve random and aligned nanofiber structures, respectively. Characterization studies confirmed successful Tranexamic acid (TXA) encapsulation and structural integrity of the scaffolds. <em>In vitro</em> cytotoxicity tests showed excellent biocompatibility, while in vivo full-thickness wound models demonstrated superior wound closure rates, enhanced collagen deposition, and elevated <em>TGF-β1</em> expression with minimal skin irritation. Notably, scaffolds with aligned fibers and thinner PLA barriers significantly accelerated healing compared to random fibers and marketed formulations. These findings highlight the promising potential of the fabricated multilayered electrospun nanofiber scaffold as an effective biodegradable wound dressing that not only accelerates hemostasis but also promotes enhanced wound healing.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"114 ","pages":"Article 107542"},"PeriodicalIF":4.9,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}