Journal of Drug Delivery Science and Technology最新文献

{"title":"Rhamnolipids in Advanced Biomaterial Engineering: Applications in antimicrobial, wound healing, and drug delivery","authors":"Aylin Çolak ,&nbsp;Gamze Dik ,&nbsp;Ahmet Ulu ,&nbsp;Hüseyin Kahraman ,&nbsp;Burhan Ateş","doi":"10.1016/j.jddst.2025.107034","DOIUrl":"10.1016/j.jddst.2025.107034","url":null,"abstract":"<div><div>Biosurfactants, widely utilized today in applications ranging from the food industry to medical fields, are among the most captivating biotechnological molecules for researchers. These remarkable molecules can be defined as amphiphilic compounds produced either on microbial cell surfaces or secreted extracellularly, comprising both hydrophobic and hydrophilic components. Among biosurfactants, rhamnolipids are the most extensively studied and widely used. Although rhamnolipids can be obtained and produced from different sources, they are predominantly obtained from <em>Pseudomonas aeruginosa</em>, whose structures are well-characterized. Rhamnolipids are important for their use in the medical field due to their many advantages such as being extremely biocompatible, exhibiting antibacterial properties, high selectivity, and stability. Specifically, synthesizing biomaterials for medical purposes using rhamnolipids or their functionalization with rhamnolipids can impart superior properties to these materials. Considering that these biomaterials will be used in clinical applications, they must exhibit antibacterial/antimicrobial properties. Furthermore, these biomaterials are highly suitable for applications such as drug delivery or wound healing. This review focuses on the preparation of rhamnolipids and applications of rhamnolipid-based biomaterials such as antimicrobial, wound healing, and drug delivery. It aims to highlight the significant potential of these materials in advancing the field of biomaterials science.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107034"},"PeriodicalIF":4.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olive leaf extract-based eye drop formulations for corneal wound healing","authors":"Chiara Migone ,&nbsp;Luca Cerri ,&nbsp;Bárbara Ferreira ,&nbsp;Angela Fabiano ,&nbsp;Sara Parri ,&nbsp;Andrea Mezzetta ,&nbsp;Lorenzo Guazzelli ,&nbsp;Anna Maria Piras ,&nbsp;Ylenia Zambito ,&nbsp;Bruno Sarmento","doi":"10.1016/j.jddst.2025.107026","DOIUrl":"10.1016/j.jddst.2025.107026","url":null,"abstract":"<div><div>Wound healing is a multifaceted process that can be enhanced through effective therapeutic strategies. This study explores the application of quaternary ammonium chitosan-methyl-β-cyclodextrin (QA-Ch-MCD) as a mucoadhesive polymer to enhance the delivery and efficacy of olive leaf extract (OLE-GS) derived from drought-stressed Italian olive cultivars (Giarraffa, Leccino, and Maurino) in corneal wound healing. QA-Ch-MCD contributes to these enhancements through its strong complexing ability, which protects the polyphenolic compounds in OLE-GS from degradation after prolonged exposure, thereby increasing their stability. Its mucoadhesive properties may reduce drug elimination from the ocular surface, thus improving bioavailability, while its intrinsic antioxidant activity complements the bioactives in OLE-GS, amplifying the overall therapeutic effect. <em>In vitro</em> scratch assays revealed that while OLE-GS promoted cell migration, its combination with QA-Ch-MCD significantly accelerated scratch closure rates compared to controls. Indeed, Notably, QA-Ch-MCD/OLE-GS demonstrated significantly greater effectiveness in promoting scratch closure compared to all other formulations tested (<em>p</em> &lt; 0.05 vs all formulations). The QA-Ch-MCD polymer improved both the stability of polyphenols, indeed polyphenols complexed with the formulation were more stable than the free ones for up to 4 h and enhanced the antioxidant activity of OLE-GS, by protecting the viability of cells exposed to oxidative damage. Furthermore, an eyedrop formulation based on QA-Ch-MCD and OLE-GS exhibited excellent cytocompatibility and <em>in vivo</em> tolerability; as demonstrated by <em>in vitro</em> cytotoxicity studies in which the formulation showed no toxicity at the concentrations of 5–10 μg/mL, and by <em>in vivo</em> studies in which no signs of ocular irritation were detected. Taken together, the results indicate that QA-Ch-MCD is an efficient functional excipient for OLE-GS in eye drop formulations. This single excipient not only provides robust protection against oxidation, preserving the stability and efficacy of the extract, but also significantly enhances its delivery. Thus, these findings highlight QA-Ch-MCD as a functional excipient that enhances the therapeutic efficacy of OLE-GS, representing a promising approach in ocular drug delivery systems for corneal wound healing<strong>.</strong></div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107026"},"PeriodicalIF":4.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct ink write 3D printed poly (ethylene oxide)/ Guar gum/ lawsone scaffolds for burn care applications","authors":"Rahila Batul ,&nbsp;Muhammad Sameet Ismat ,&nbsp;Muhammad Haseeb Nawaz ,&nbsp;Syed Muneeb Haider Gillani ,&nbsp;Aqsa Aizaz ,&nbsp;Adam Khan ,&nbsp;Huzaifa Shafique ,&nbsp;Mian Sami Mudassir ,&nbsp;Sheraz Ahmed ,&nbsp;Fatima Javed ,&nbsp;Abdul Khaliq ,&nbsp;Muhammad Atiq Ur Rehman","doi":"10.1016/j.jddst.2025.107022","DOIUrl":"10.1016/j.jddst.2025.107022","url":null,"abstract":"<div><div>Burn is one of the leading causes of trauma worldwide, with significantly higher mortality rates in third-world countries. Therefore, there is an urgent need to provide dynamic solutions to treat burns, particularly for third-degree burn patients. The ideal skin patch should exhibit excellent water retention, mechanical strength, regeneration potential (angiogenesis), and inhibition to the growth of the bacteria. Herein, we proposed a 3D printed lawsone loaded polyethylene oxide (PEO)/Guar gum (GG) scaffold as a potential candidate for burn care application. This study mainly focuses on the synthesis, material characterization, lawsone release kinetics, scaffold degradation and cytocompatibility with the human dermal fibroblast cells. The lawsone-loaded PEO/GG scaffolds exhibited favorable porosity for skin regeneration application. Fourier Transform Infrared analysis confirmed the crosslinking between PEO and GG via C-O-C linkage and incorporated lawsone. The degradation kinetics of the fabricated scaffolds were studied in order to confirm their suitability for skin regeneration applications. The lawsone was released in a sustained manner (∼64 % in 168 h) owing to the cross-linking between PEO and GG. The sustained release of Lawsone can provide long-term therapeutic effects. Moreover, the chorioallantoic membrane assay confirmed the pro-angiogenic behavior of the PEO/GG/lawsone scaffold. The fabricated scaffold inhibited the growth of bacterial strains, i.e., <em>Escherichia coli</em> and <em>Staphylococcus aureus.</em> Moreover, the fabricated PEO/GG and PEO/GG/lawsone scaffolds showed cytocompatibility to primary dermal fibroblasts. The results obtained during the initial <em>in-vitro</em> studies suggested that the PEO/GG scaffolds loaded with lawsone can further be tested under <em>in-vivo</em> conditions.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107022"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molded all-in-one microneedles to administer diverse drugs in clinical biomedical field","authors":"Wenjun Wang ,&nbsp;Zhou Wang ,&nbsp;Gaofei Zeng ,&nbsp;Zhikun Wang ,&nbsp;Lu Xia ,&nbsp;Yi Zhang","doi":"10.1016/j.jddst.2025.107029","DOIUrl":"10.1016/j.jddst.2025.107029","url":null,"abstract":"<div><div>Microneedle technologies, owing to their minimally invasive nature, can be used to administer various drugs and could be beneficial for numerous applications in the healthcare and biomedical fields. Molded microneedles featuring carriers and drugs in one single unit have been identified as traditional microneedles. Molded microneedles with various dimensions and micro-nano structures can be fabricated from numerous materials, typically using one to three casting solutions filled into a microneedle mold with multi-stage reactions followed by centrifugation or vacuum treatment, drying, curing, and demolding. This review examines the fabrication methodologies of various molded microneedle architectures, with comparison of their material properties and applications performance characteristics. This paper also enumerates the clinical biomedical applications of molded microneedles (e.g., transdermal administration, gastrointestinal therapies, and oral health). Future research efforts with respect to clinical translation are also discussed.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"109 ","pages":"Article 107029"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and anti-tumor migration potency of biosynthesized gold/silver alloy nanoparticles mediated by polydopamine","authors":"Zihan Wang ,&nbsp;Xue Sha ,&nbsp;Wenyan Li ,&nbsp;Qiulian Song ,&nbsp;Jiayu Guo ,&nbsp;Honglei Zhan ,&nbsp;Tao Liu","doi":"10.1016/j.jddst.2025.107031","DOIUrl":"10.1016/j.jddst.2025.107031","url":null,"abstract":"<div><div>Bacterial infection is a leading cause of death, and has been increasingly associated with cancer development. Thus, developing nanomaterials with both antibacterial and anticancer properties is crucial. Antibiotics are mono-functional and their misuse can lead to the development of antibiotic-resistant bacteria and ecological problems. This study developed the biosynthesis of gold-silver alloy nanoparticles (Au/AgNPs) using polydopamine (PDA) as a multifunctional agent, combining high photothermal efficiency and biocompatibility of gold, along with the antibacterial and anticancer properties of silver, while mitigating its toxicity. These nanoparticles were synthesized and optimized for size, morphology, SPR peak, stability, crystallinity, and photothermal performance, and characterized using SEM, TEM, DLS, UV–Vis spectroscopy, and XRD analysis. In addition, their antibacterial performance and underlying mechanism were studied by MIC determination, inhibition zone test, SEM imaging and RT-PCR analysis. Their anti cancer cell migration effect was also evaluated by a wound healing assay. Major results indicated that optimized Au/AgNPs exhibited quasi-spherical morphology (153.14 ± 3.62 nm) with multiple twinning structures, uniform dispersion, and excellent photothermal performance. XRD analysis showed a lattice constant of 4.7154 Å with 185 nm crystallite size and 0.108 strain value. They exhibited enhanced antibacterial efficacy under NIR irradiation, achieving over 80 % inhibition of both Gram-positive and Gram-negative bacteria at a concentration of 6.04 μg/mL. Simultaneously, they downregulated the expression of <em>PBP2</em> and <em>MurB</em> by 30–40 %. This outstanding performance stems from the synergistic effect of photothermal/chemotherapy therapy. Moreover, tumor targeting peptide (YL) decorated Au/AgNPs significantly inhibited invasive MDA-MB-231 cell migration, reducing the rate to only 1.12 %. In addition, they demonstrated 3.4 times higher biocompatibility than AgNPs. This dual-action platform merged PTT and metal ion chemotherapy, offering a targeted, biocompatible strategy to concurrently combat antibiotic-resistant infections and cancer metastasis, which is of great significance for the progress of novel antibacterial and antitumor combined therapy drugs.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"109 ","pages":"Article 107031"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in targeted therapy on cancers by nanoparticles encapsulated in exosomes: a comprehensive review","authors":"Wajida Ataallah Khidr ,&nbsp;Karar H. Alfarttoosi ,&nbsp;Waam Mohammed Taher ,&nbsp;Mariem Alwan ,&nbsp;Ali M. Ali Al-Nuaimi ,&nbsp;Mahmood Jasem Jawad","doi":"10.1016/j.jddst.2025.107033","DOIUrl":"10.1016/j.jddst.2025.107033","url":null,"abstract":"<div><div>With the advent of nanotherapeutic medications in the realms of anti-tumor multidrug resistance (MDR) and drug delivery systems, new opportunities for combination therapy and the elimination of drug resistance mechanisms have emerged. The formulation loses all efficacy in controlling cancer cell growth when nanoparticles (NPs) agglomerate within cells to form a protein corona, which interferes with the biomolecules' normal action. Negative interactions between NPs and living things may cause dangerous toxicities. New possibilities for nanomedicine have emerged thanks to the fast development of nanotechnology based on extracellular vesicles. After merging with the plasma membrane, the multivesicular body (MVB) releases its contents into the extracellular environment, producing the exosome (EXO). In addition, EXOs has been engineered to include a wide range of therapeutic compounds, including nucleic acids. A number of these methods have shown promising results in combating diverse illnesses. Because receptor proteins and lipids on recipient cells allow EXOs to concentrate cargo distribution to particular cells or regions, EXOs may also improve delivery efficiency. More so, the tiny size of EXOs, coupled with their enhanced permeability and retention (EPR) function, allows them to target cancer areas. We have compiled a list of all the NPs employed to cure cancer. Then, we reviewed EXOs for targeted therapy and NP side effect reduction in response to clinical limits in NP cancer treatment. In this paper, we have discussed the key features of EXOs and evaluated current research on using EXO-encapsulated anticancer NPs to treat cancer. At last, we have covered the benefits and drawbacks of this approach to cancer therapy in clinical settings, as well as key ways to address the latter.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107033"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticle organelle targeting kinetics: a discussion on application","authors":"Niki Pouyanfar ,&nbsp;Parnia Aftabi ,&nbsp;Golrokh Farnam ,&nbsp;Niloofar Masoumi ,&nbsp;Farshad H. Shirazi ,&nbsp;Fatemeh Ghorbani-Bidkorpeh","doi":"10.1016/j.jddst.2025.107028","DOIUrl":"10.1016/j.jddst.2025.107028","url":null,"abstract":"<div><h3>Background</h3><div>Many diseases originate from specific cell organelles vital to cell function and survival. In these cases, verification of drug arrival to the relevant subcellular areas may contribute significantly to faster recovery and ailment eradication. Organelle targeting is a multi-factorial issue that, if addressed, can ease the path toward treating many diseases. For this purpose, nanotechnology ensures that a drug carrier with optimal physicochemical and biological effects is designed to have better effects. Meanwhile, studying this process's kinetics may contribute to better nano-carrier design and characterization.</div></div><div><h3>Aim</h3><div>This review gathers various subcellular targeting strategies and discusses the application of kinetics in a superior nano-drug delivery system development. Lastly, different organelles are explored, tackling the challenges of kinetics and uptake.</div></div><div><h3>Methods</h3><div>Different databases (Scopus, Google Scholar, and Pubmed) were searched with various keywords such as “kinetics”, “cellular uptake”, “sub-cellular”, “organelle”, “nanoparticle” and organelle titles (nucleus, lysosome, mitochondria, endoplasmic reticulum, and Golgi apparatus).</div></div><div><h3>Results</h3><div>The review discussed organelle targeting methods along with the utilization of uptake kinetics in vital cell compartments.</div></div><div><h3>Conclusions</h3><div>Understanding nanoparticle sub-cellular uptake kinetics will be of great use in the application of such nanostructures for drug delivery and in understanding the critical scale-up parameters for future clinical utilization. Enhanced therapeutic outcomes, fewer adverse effects, controlled drug release, and even the creation of improved imaging techniques may be among the several consequences that follow implementing optimal organelle targeting kinetics using nanoparticles.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"109 ","pages":"Article 107028"},"PeriodicalIF":4.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel solid self-emulsifying drug delivery system to improve the physical performance and bioavailability of supercritical fluid extracts of frankincense and myrrh","authors":"Wansi Li ,&nbsp;Panwen Huang ,&nbsp;Xianghuan Sun ,&nbsp;Yongtai Zhang ,&nbsp;Ying Liu ,&nbsp;Nianping Feng ,&nbsp;Zhi Wang","doi":"10.1016/j.jddst.2025.107030","DOIUrl":"10.1016/j.jddst.2025.107030","url":null,"abstract":"<div><div>Frankincense and myrrh are commonly used in traditional Chinese medicines, and clinical studies have shown that their combined application has a good therapeutic effect on inflammation and tumors. In this study, the supercritical fluid extract of frankincense and myrrh (SFE-FM) was prepared into frankincense and myrrh solid self-emulsifying drug delivery system (FM S-SEDDS) mini-tablets. SFE-FM was used as the main oil phase, and the self-microemulsion was screened using a pseudo-ternary phase diagram and a central composite design. Neusilin was used as the carrier and coating material, with HPMC K100M serving as the matrix material to prepare the sustained-release mini-tablets. Characterization techniques, including scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and Fourier-transform infrared (FTIR) analysis, assessed the FM-SEDDS powders, with evaluations of tensile strength (TS), content uniformity, <em>in vitro</em> release, and <em>in vivo</em> pharmacokinetic conducted on the tablets. The FM-SEDDS had an average particle size of 22.36 nm, a polydispersity index (PDI) of 0.23, and a zeta potential of −1.66 mV, with stable properties for 30 d room temperature. It demonstrated suitable tableting properties, including flow, compressibility, and compatibility, while retaining its self-microemulsifying capabilities. <em>In vitro</em> testing confirmed complete release of acetyl-11-keto-<em>β</em>-boswellic acid (AKBA), <em>β</em>-boswellic acid (<em>β</em>-BA), and <em>β</em>-elemonic acid (<em>β</em>-EA) compared to blank tablets. Pharmacokinetic results in Beagle dogs indicated a significant sustained-release effect for the three active ingredients with a strong <em>in vitro</em>–<em>in vivo</em> correlation (IVIVC). SEDDS technology can improve the oral bioavailability of liposoluble drugs like SFE-FM, offering promising industrial application potential.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"109 ","pages":"Article 107030"},"PeriodicalIF":4.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and characterization of As2O3 incorporated into magnetic graphene oxide derivatives: Biological activity in keratinocytes and melanoma cells","authors":"Altevir Rossato Viana ,&nbsp;Alice Penteado Holkem ,&nbsp;Franciele da Silva Bruckmann ,&nbsp;Nickolas Pippi ,&nbsp;Leonardo Vidal Zancanaro ,&nbsp;Sergio Roberto Mortari ,&nbsp;Erico Marlon Moraes Flores ,&nbsp;Cristiano Rodrigo Bohn Rhoden ,&nbsp;André Passaglia Schuch","doi":"10.1016/j.jddst.2025.107023","DOIUrl":"10.1016/j.jddst.2025.107023","url":null,"abstract":"<div><div>Cancer is the second leading cause of death worldwide, and melanoma is the type of skin tumor with the worst prognosis. Traditional oncology treatments cause numerous adverse effects, as they affect proliferating cells throughout the body. Therefore, this study evaluated the chemical synthesis and biological activity of the chemotherapeutic drug Arsenic Trioxide (As₂O₃), incorporated into graphene oxide (GO) containing different amounts of magnetite (Fe₃O₄) covering its surface (1:1 and 1:10). XRD, FTIR, SEM, and EDS techniques were performed to characterize GO·Fe₃O₄-As₂O₃. Initially, the IC50 was determined in B16-F10 (melanoma) and HaCat (keratinocytes) cell lines using the MTT assay for all treatments, which had a concentration range between 10 and 300 μg mL⁻¹, and subsequently, dsDNA PicoGreen, Colony Formation, DCFH-DA, NO, and Superoxide were evaluted. Experimental data showed that the synthesized material contained complexed As₂O₃. Regarding the biological assays, free As₂O₃ was toxic to the tested cells; however, the most significant IC₅₀ results were 557 μg mL⁻¹ and 75 μg mL⁻¹ for HaCat and B16-F10, respectively, in the treatment with (GO·Fe₃O₄ 1:1)-As₂O₃. The antiproliferative activity results also showed a strong decrease in colony formation, except for (GO·Fe₃O₄ 1:1)-As₂O₃ in non-tumor cells. The antiproliferative activity results also showed a strong decrease in colony formation, except for (GO·Fe₃O₄ 1:1)-As₂O₃ in non-tumor cells. ROS production was generally significant only at higher concentrations containing As₂O₃, while none of these treatments elevated NO levels remained unaffected. Superoxide levels were significantly elevated compared to the untreated control. These findings suggest that GO·Fe₃O₄-As₂O₃ nanocomposites hold promise as a targeted therapeutic approach for melanoma, offering improved cytotoxic selectivity and potential for further biomedical applications.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"109 ","pages":"Article 107023"},"PeriodicalIF":4.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the solubility and bioavailability of Bazedoxifene with varying γ-cyclodextrin metal-organic frameworks (γ-CD-MOFs) as delivery vehicles","authors":"Peng Wang,&nbsp;Yue Ma,&nbsp;Lihua Wei,&nbsp;Li Miao,&nbsp;Xiaoxia Wang,&nbsp;Wen Chen","doi":"10.1016/j.jddst.2025.106982","DOIUrl":"10.1016/j.jddst.2025.106982","url":null,"abstract":"<div><div>To address the poor solubility and low bioavailability of BCS Class II drugs, exemplified by Bazedoxifene (BZA), in aqueous environments, we utilized γ-cyclodextrin (γ-CD), γ-cyclodextrin metal-organic frameworks (3D-CD-MOFs), and γ-cyclodextrin metal-organic framework nanosheets (2D-CD-MOFs) as carriers for BZA, and investigated their effects on the drug's solubility and bioavailability. The synthesis and drug-loading processes were optimized, and the impact on the morphology and properties of the CD-MOFs was explored. Molecular docking simulations were conducted to examine the distribution and binding sites of BZA within the CD-MOFs. Finally, the drug loading capacity, solubility, in vitro release, and pharmacokinetics were assessed. The experimental results indicated that the 2D-CD-MOF possessed a high BZA loading capacity and significantly improved drug solubility and bioavailability. The bioavailability of BZA@2D-CD-MOF was 4.47, 1.38 and 4.41 times higher than that of BZA, BZA@3D-CD-MOF and BZA@γ-CD groups, respectively. This study demonstrates that 2D-CD-MOF nanosheets significantly outperform 3D-CD-MOFs and γ-CD in enhancing BZA solubility and bioavailability, exhibiting favorable safety profiles. Employing γ-cyclodextrin metal-organic framework nanosheets for BZA loading presents considerable feasibility and potential for enhancing its solubility and bioavailability.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 106982"},"PeriodicalIF":4.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}