Journal of Drug Delivery Science and Technology最新文献

{"title":"Characterization and osteogenic potential of caffeic acid-enriched alginate/poly(vinyl alcohol) composite hydrogels for bone tissue engineering","authors":"Reshma Jolly ,&nbsp;Shruti Mahapatra ,&nbsp;Kuldeep ,&nbsp;G.A. Basheed ,&nbsp;Sujata Mohanty ,&nbsp;Mohammad Shakir ,&nbsp;Nahar Singh","doi":"10.1016/j.jddst.2025.107051","DOIUrl":"10.1016/j.jddst.2025.107051","url":null,"abstract":"<div><div>Interest in the development of composite hydrogels having adequate biodegradability, biocompatibility and mechanical properties has been rekindled with the advent of hybrid biomaterials for bone regeneration applications. This is the first report where caffeic acid (CA) is introduced in sodium alginate (SA)/poly(vinyl alcohol) (PVA) polymer domain in the order of increasing concentration of CA to obtain SC[0,100,150,200] composite hydrogels by ionic gelation. The FTIR and XRD confirmed the presence of various components and anticipated interactions among them. The AFM and SEM analyses recorded the rougher surface topography in case of SC[100,150,200] composite hydrogels in comparison to SC0 which is used as control. The maximum roughness (Sq) was significantly increased to 27.93 nm with ten point height (Sz) value as 109.45 nm in case of the SC200 with highest percentage of CA. The hydrogels were cytocompatible as assessed by MTT assay and Live/Dead staining. The gene profiling of Osteogenic markers was investigated by real-time PCR. The SC200 displayed significant viability, proliferation, and higher capability of differentiating WJ-MSCs (Wharton's Jelly-derived Mesenchymal Stem Cells) into osteocytes as evident by the heightened expression of OCN (Osteocalcin), BMP-4 (Bone Morphogenetic Protein), and Col-I (Collagen). Given the favorable physical and biological properties observed in case of SC200, it is evident that it stands out as a strong contender for addressing bone defects.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107051"},"PeriodicalIF":4.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Memantine loaded micro/nanoscale magnetic motors for the treatment of Alzheimer's Disease","authors":"Gizem Tezel Temel ,&nbsp;Elif Öztürk ,&nbsp;Sıla Ulutürk ,&nbsp;Süleyman Can Öztürk ,&nbsp;Tuba Reçber ,&nbsp;Selin Seda Timur ,&nbsp;Emirhan Nemutlu ,&nbsp;Filiz Kuralay ,&nbsp;Güneş Esendağlı ,&nbsp;R. Neslihan Gürsoy ,&nbsp;Levent Öner ,&nbsp;Hakan Ceylan ,&nbsp;Hakan Eroğlu","doi":"10.1016/j.jddst.2025.107043","DOIUrl":"10.1016/j.jddst.2025.107043","url":null,"abstract":"<div><div>Micro/nanomotors (MNM) are self-propelled devices operating at the nano or microscale, offering significant potential for applications such as drug delivery, cell manipulation, bio-imaging, diagnostics, and environmental remediation. In this study, we report for the first time the development of novel, magnetically actuated MNMs loaded with Memantine (Mem) toward the treatment of Alzheimer's Disease (AD). AD, a neurodegenerative disease with no cure, presents a significant challenge due to its increasing prevalence. This research aimed to design a targeted drug delivery system using Mem, a common AD treatment, loaded onto magnetically maneuverable MNMs. The fabricated Mem-loaded MNMs were thoroughly characterized in terms of morphology, particle size, drug loading efficiency, magnetic actuation and in vitro drug release kinetics. The final formulation exhibited a drug loading efficiency of 14.06 ± 5.2 % and achieved 95 ± 2.4 % drug release within 72 h. The average velocity of the Mem loaded magnetically propelled MNMs was determined as 16.3 ± 2.3 μm/s. Cytotoxicity assessments in SH-SY5Y cells confirmed the non-toxicity of the MNM formulation (77.08 ± 2.5 % viability at 48 h), while efficacy studies demonstrated significant BACE1 inhibition and TrkB upregulation, with superior blood brain barrier (BBB) model suppression versus Mem solution, indicating potential for enhanced BBB drug delivery and therapeutic benefit. Metabolomic analysis corroborated these findings, showing alterations in glutathione, asparagine, phosphatidylcholine, and phosphatidylinositol levels, consistent with AD-related metabolic changes. <em>In vivo</em> biodistribution in Balb-C mice showed significant MNM brain accumulation within 30 min. Altogether, this study suggests that magnetically actuated, Mem-loaded MNMs represent a promising strategy for leveraging efforts in managing Alzheimer's Disease through enhanced, site-specific drug delivery approaches.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107043"},"PeriodicalIF":4.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative transdermal delivery of microneedle patch for dual drugs febuxostat and lornoxicam: In vitro and in vivo efficacy for treating gouty arthritis","authors":"Sammar Fathy Elhabal ,&nbsp;Hossam Abdo Ashour ,&nbsp;Mohamed Fathi Mohamed Elrefai ,&nbsp;Mahmoud H. Teaima ,&nbsp;Nahla A. Elzohairy ,&nbsp;Nada ahmed kholeif ,&nbsp;Mohamed El-Nabarawi","doi":"10.1016/j.jddst.2025.107053","DOIUrl":"10.1016/j.jddst.2025.107053","url":null,"abstract":"<div><div>Gouty arthritis (GA) is a kind of joint disease caused by the deposition of monosodium urate (MSU) crystals. Anti-inflammatory and uric acid-lowering treatments are necessary for GA patients on a long-term basis. Febuxostat (Feb), a selective xanthine oxidase inhibitor, with a moderate F value (&lt;49 %). Lornoxicam (Lor) is a potent nonsteroidal anti-inflammatory drug (NSAID) that is widely used to treat pain and inflammation. However, both drugs belong to BCS class II, showing low solubility and high permeability. This study presents a microneedle (MN) patch for the co-delivery of Febuxostat and Lornoxicam to tackle the challenges associated with gouty arthritis treatment. The optimized microneedle patch with a biodegradable polyvinyl alcohol (PVA)/polyvinylpyrrolidone (PVP), polyethene glycol (PEG 400) matrix and chitosan backing worked well both <em>in vitro</em> and <em>in vivo</em> study. Lornoxicam and Febuxostat were released at 99 % and 100 %, respectively, <em>in vitro</em>, within 48 h. The microneedles were strong enough to withstand 1000 g but disintegrated within minutes of skin contact. <em>In vivo</em> studies utilizing a hyperuricemia rat model demonstrated that the microneedle patch decreased serum uric acid levels by 85.4 % and inhibited xanthine oxidase activity in the serum and liver by 72.4 % and 68.3 %, respectively. The patch significantly reduced inflammatory markers like MMP-3, CRP, TNF-α, and IL-1β, demonstrating its anti-inflammatory properties. Histopathological analysis showed greater joint and hepatic architecture restoration and inflammation reduction than conventional formulations. This novel microneedle patch delivery method outperformed oral and gel-based drug delivery methods in bioavailability, therapeutic effects, and systemic side effects. These results demonstrate the microneedle-based system's potential as an innovative and patient-friendly, gouty arthritis treatment.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107053"},"PeriodicalIF":4.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yeast-Originated drug Carriers: Comprehensive study from preparation to functional evaluation","authors":"Fan Yang,&nbsp;Shang Shang,&nbsp;Mengfei Qi,&nbsp;Meiyi Hou,&nbsp;Yajinjing Xiang,&nbsp;Jiayan Shen,&nbsp;Ying Tong,&nbsp;Yan Zhang,&nbsp;Jia Liu,&nbsp;Qing Wu","doi":"10.1016/j.jddst.2025.107049","DOIUrl":"10.1016/j.jddst.2025.107049","url":null,"abstract":"<div><div>Yeast glucan particles (YGPs), natural drug delivery carriers derived from yeast cells, exhibit negative charges, enabling electrostatic drug binding. Their <em>β</em>-glucan-rich surface demonstrates substantial biological activity and recognition by macrophage Dectin-1 receptors, promising for development. Nevertheless, as a natural material, the pretreatment process for yeast microcapsules has yet to undergo systematic exploration, resulting in diverse process parameters. This study aims to optimize the fabrication process of yeast microcapsules, focusing on enhancing their cavity capacity and electrostatic adsorption abilities, thereby improving their loading capacity for positively charged drugs. Our objective is to provide a standardized protocol for subsequent applications, facilitating the advancement of yeast-based drug delivery systems. YGPs were prepared via an alkali-acid-organic solvent method, employing various process parameters. The encapsulation efficiency and loading capacity of YGPs for target drugs (positively charged Berberine) served as primary indices. By analyzing the changes in Zeta potential under different process conditions, the optimal acid-alkali treatment temperatures were determined to be 70 °C and 60 °C, respectively, with an optimal treatment duration of 1 h. Processed YGPs can effectively shield their active ingredient (Berberine) from gastrointestinal degradation and delay its release. The optimally processed yeast microcapsules exhibited a Zeta potential of −10.8 mV, a <em>β</em>-glucan content of (73.32 ± 3.18) %, and substantial internal drug-carrying capacity without inducing hemolysis. Furthermore, the ability of these microcapsules to be specifically recognized and phagocytosed by macrophages via the Dectin-1 receptor was confirmed, underscoring their potential for macrophage-targeted <em>in vivo</em> applications. This study provides both experimental insights and a foundation for the investigation of oral drug delivery carriers.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107049"},"PeriodicalIF":4.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotechnology in photodynamic therapy: A targeted approach for the treatment of topical fungal infections","authors":"Gabriela Lopes Gama e Silva ,&nbsp;Bruna Coelho de Almeida ,&nbsp;Isabella da Costa Martins ,&nbsp;Clara Dias ,&nbsp;Ralph Santos-Oliveira ,&nbsp;Eduardo Ricci-Júnior","doi":"10.1016/j.jddst.2025.107047","DOIUrl":"10.1016/j.jddst.2025.107047","url":null,"abstract":"<div><div>Cutaneous fungal infections are among the most prevalent clinical conditions in humans, with candidiasis, caused by Candida species, being the most common. The rise in antimicrobial resistance and the significant adverse effects associated with conventional therapies highlight the urgent need for novel treatment strategies. One promising approach is photodynamic therapy, which employs light-sensitive agents and a specific irradiation source to target and eliminate fungal infections in a localized manner. However, solubility challenges hinder the development of effective formulations. To address this, light-sensitive agents can be incorporated into nano-sized delivery systems. This article aims to evaluate the potential benefits of this combination and its impact on enhancing antifungal efficacy in both <em>in vitro</em> and <em>in vivo</em> models. Experimental studies were selected from three databases, focusing exclusively on preclinical research. The findings indicate that nanoparticles were the most studied nanosystem, and the phthalocyanine class of photosensitizers demonstrated significant efficacy against <em>Candida albicans</em>. Further investment is essential to advance clinical studies and facilitate the eventual integration of these therapies into clinical practice and the pharmaceutical market.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107047"},"PeriodicalIF":4.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on revolutionizing pancreatic cancer Treatment: Liposomal innovations","authors":"Rohit Sharma,&nbsp;Km Komal,&nbsp;Sourabh Kumar,&nbsp;Rashmi Ghosh,&nbsp;Manish Kumar","doi":"10.1016/j.jddst.2025.107032","DOIUrl":"10.1016/j.jddst.2025.107032","url":null,"abstract":"<div><div>Pancreatic cancer (PC) is a lethal disease characterized by a poor prognosis and limited treatment efficacy. Approximately 90 % of pancreatic tumors are classified as pancreatic ductal adenocarcinoma (PDAC), making it the most prevalent type of PC. Various conventional treatments are available for PC, such as surgery, chemotherapy, radiodynamictherapy, phototherapy and supportive care. Despite their utilization, their effectiveness is limited by several drawbacks such as toxicity, resistance to treatment, and damage to healthy tissues. Liposomes have emerged as a promising solution to enhance the therapeutic effectivenessof various chemotherapeutic agents for PC. Various liposomes, including PEG-coated, pH-sensitive, thermosensitive, enzyme-responsive, ultrasound-sensitive, ligand-targeted, and magnetic liposomes, have demonstrated great potential in preclinical studies. Although various clinical trials are conducted on liposomal formulations, they reveal the therapeutic potential of liposomes for clinical utility. The patent landscape is also studied to get insights into advancements and improvements in the research area. Overall, the liposomes have great potential to enhancethe delivery of drugs by encapsulating both lipophilic and hydrophilic drugs, possessing targeted delivery to tumor sites, offering improvement in bioavailability, reducing adverse effects, overcoming drug resistance, and enhancing the therapeutic outcomes. This review highlights the potential of liposomes to transform PC therapy by enabling target delivery, reducing off-target effects, and enhancing therapeutic efficacy to improve patient outcomes.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107032"},"PeriodicalIF":4.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Norepinephrine-loaded thermoresponsive hydrogel-like system for enhanced angiogenesis in tissue engineering","authors":"Tassia Joi Martins ,&nbsp;Italo Rodrigo Calori ,&nbsp;Ana Paula Pereira Guimaraes ,&nbsp;Gabriel Henrique Ribeiro ,&nbsp;Tiago Venâncio ,&nbsp;Fiama Martins ,&nbsp;Lucas da Silva Ribeiro ,&nbsp;Emerson Camargo ,&nbsp;Antonio Claudio Tedesco","doi":"10.1016/j.jddst.2025.107046","DOIUrl":"10.1016/j.jddst.2025.107046","url":null,"abstract":"<div><div>Inadequate vascularization in thick biofabricated tissues and organs leads to hypoxia and graft failure, highlighting the critical role of angiogenesis in the clinical translation of tissue engineering. Norepinephrine (NE), a neurotransmitter and hormone, regulates vascular tone and modulates angiogenesis through proangiogenic factors, offering an option for improving vascularization and tissue integration. This study aimed to characterize an injectable and <em>in situ</em> forming hydrogel-like system made of poly(N-vinylcaprolactam) (PNVCL) loaded with NE, and evaluate its effects on endothelial cell models. PNVCL was successfully synthesized via free-radical polymerization, as indicated by ¹H NMR and FTIR spectroscopy. The polymer exhibited a glass transition temperature (Tg) of 194 °C and thermoresponsive behavior with a lower critical solution temperature of 34 °C. NMR spectroscopy indicated through-space dipolar couplings between NE and PNVCL hydrogens, which suggest interaction groups within the NE-PNVCL system. This system protects NE from degradation, as indicated by absorption spectroscopy. The PNVCL system exhibited a burst release of NE followed by a controlled release profile, with approximately 80 % and 87 % of the NE released within 24 h and 72 h, respectively. In human umbilical vein endothelial cells (HUVEC) culture, NE reduced migration and enhanced tubulogenesis, indicating a dual role in angiogenesis. In addition, NE promoted sprout formation in endothelial cell spheroids. These findings highlight the potential of NE-loaded PNVCL systems to enhance angiogenesis and vascularization in tissue engineering.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107046"},"PeriodicalIF":4.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic mediated emulsification for drug delivery applications","authors":"Amarah Sultan Rana ,&nbsp;Sadaf Sarfraz ,&nbsp;Mohamed H. Helal ,&nbsp;Azwan Mat Lazim ,&nbsp;Mohammed A. Amin ,&nbsp;Mahmoud M. Hessien ,&nbsp;Muhammad Faizan Nazar","doi":"10.1016/j.jddst.2025.107045","DOIUrl":"10.1016/j.jddst.2025.107045","url":null,"abstract":"<div><div>Conventional drug delivery methods in life sciences and patient care often have excessive problems of homogeneity and batch-to-batch variability. Microfluidics technology has gained significant attraction due to the miniaturization of the fluid environment in pharmaceuticals and biomedicine, offering solutions to the problems associated with drug delivery. The diverse applications of microfluidic technology include emulsification, a process for producing stable suspensions of one immiscible liquid in another, which is particularly suitable for improving drug delivery systems. Miniaturization of emulsification in microfluidic devices offers several advantages, including better control of droplet size, improved reproducibility, and improved scalability. Microfluidic-mediated emulsification yields monodisperse droplets with a narrow size distribution, which play a key role in drug delivery by ensuring uniform drug dosage and release kinetics, thus minimizing the risk of over- or under-dosing. Moreover, microfluidic-mediated emulsification techniques such as flow-focusing and droplet-based microfluidics enable precise control over the geometry of the droplets, enabling tailored drug delivery systems. The inherent scalability of microfluidic systems makes them suitable for large-scale production of emulsions suitable for pharmaceutical applications, while controlled emulsification in microfluidic devices also facilitates the encapsulation of amphiphilic drugs into single-carrier systems. This versatility expands the range of drug compounds that can be effectively delivered and overcomes challenges associated with the solubility and stability of certain drugs. Therefore, emulsification in microfluidic devices offers a promising advancement in drug delivery applications. This review aims to provide an overview of microfluidic-mediated emulsification and its applications in drug delivery systems to investigate its efficiency and effectiveness in drug delivery systems and pave the way for new therapeutic strategies. Finally, this review highlights the existing patents and the evolving market landscape of microfluidic-based formulations and devices while also addressing the current challenges and limitations of microfluidic-based devices.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107045"},"PeriodicalIF":4.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study of bone differentiation and periapical bone repair induced by quercetin cyclodextrin inclusion complexes","authors":"Fan Yang,&nbsp;Jie Wang,&nbsp;Jingjing Wang,&nbsp;Xinyue Yang,&nbsp;Hongyan Zhang","doi":"10.1016/j.jddst.2025.107042","DOIUrl":"10.1016/j.jddst.2025.107042","url":null,"abstract":"<div><div>Quercetin (Qc) is a natural flavonoid recognized for its diverse biological activities but is poorly soluble in water. Hydroxypropyl beta-cyclodextrin (HP-β-CD), a cyclodextrin analog, serves as a safe and efficient drug carrier by forming clathrates with therapeutic agents, enhancing their physicochemical and pharmacokinetic properties. This study reports the synthesis of inclusion complexes of HP-β-CD and Qc using ultrasonic magnetic stirring followed by lyophilization. These complexes were analyzed using X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and UV–Vis spectroscopy (UV), Fourier-Transform Infrared spectroscopy (FTIR), and Nuclear Magnetic Resonance (NMR) to elucidate their structural and physicochemical properties. The findings validated the successful formation of clathrates between Qc and HP-β-CD, with the resulting inclusion complexes comprising Qc/HP-β-CD demonstrating markedly distinct spectral characteristics compared to unmodified Qc. Moreover, studies revealed a substantial enhancement in the anti-inflammatory and osteogenic activities of Qc upon encapsulation. These findings demonstrate that the Qc/HP-β-CD inclusion complexes markedly enhanced Qc bioavailability while amplifying its therapeutic efficacy in inflammation and bone regeneration.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107042"},"PeriodicalIF":4.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A bifunctional drug delivery system with targeting and glutathione-responsivity amphiphiles for effective tumor therapy","authors":"Dan Ma ,&nbsp;Hai Zhang ,&nbsp;An-ru Liao ,&nbsp;Qian-rong Peng ,&nbsp;Min Yang","doi":"10.1016/j.jddst.2025.107041","DOIUrl":"10.1016/j.jddst.2025.107041","url":null,"abstract":"<div><div>Drug delivery systems utilizing tumor targeting, microenvironment responsiveness, and combination therapy have demonstrated advantages in antitumor research by enhancing efficacy and reducing side effects. In this study, a bifunctional amphiphilic carrier material (HA-SSS-MC) with targeting and glutathione-responsive properties was developed by linking hydrophilic hyaluronic acid (HA) and hydrophobic methyl cholate (MC) via a trisulfide bond. The photosensitizer zinc phthalocyanine (ZnPc) and the chemotherapeutic drug 5-fluorouracil (5-FU) were encapsulated within the amphiphiles to form dual drug-loaded nanomicelles (HA-SSS-MC/5-FU/ZnPc). These nanomicelles exhibited high drug loading efficiency, stability, and glutathione (GSH)-sensitive release properties. Notably, the singlet oxygen quantum yield of the drug-loaded nanomicelles was significantly higher than that of the free photosensitizer under high glutathione conditions. In vitro cellular assays confirmed that HA-SSS-MC/5-FU/ZnPc nanomicelles exhibited low cytotoxicity and demonstrated a significant cancer cell-killing effect, surpassing Free 5-FU or ZnPc alone. Furthermore, the nanomicelles showed targeting capabilities and accumulated within endosomes. In A549 tumor-bearing mice, HA-SSS-MC/5-FU/ZnPc exhibited the strongest antitumor efficacy and the lowest toxicity compared to all other groups. In conclusion, the targeted GSH-responsive dual drug-carrying nanomicelles will be a promising strategy for cancer therapy.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"110 ","pages":"Article 107041"},"PeriodicalIF":4.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}