Journal of Dental Sciences最新文献

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The investigation of effects, signal pathways, and applications of high glucose on dental pulp stem cells 高糖对牙髓干细胞的作用、信号通路及应用研究
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-12 DOI: 10.1016/j.jds.2025.03.038
Shih-Yu Lee , I-Hsun Li , Wei-Cheng Tsai , Ming-Hua Ho , Chung-Hsing Li
{"title":"The investigation of effects, signal pathways, and applications of high glucose on dental pulp stem cells","authors":"Shih-Yu Lee ,&nbsp;I-Hsun Li ,&nbsp;Wei-Cheng Tsai ,&nbsp;Ming-Hua Ho ,&nbsp;Chung-Hsing Li","doi":"10.1016/j.jds.2025.03.038","DOIUrl":"10.1016/j.jds.2025.03.038","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Dental pulp stem cells (DPSCs) are among the most widely used dental-derived mesenchymal stem cells (MSCs), and their applications have involved various regions. The glucose metabolism plays a key role in cell function and the current literature presents conflicting evidence regarding the influence of glucose on MSCs' properties. This study evaluated the impact of high glucose (HG) on DPSCs.</div></div><div><h3>Materials and methods</h3><div>DPSCs were stimulated with indicated concentrations of glucose. Cell viability was assessed using a cell counting kit, while apoptosis and autophagy were analyzed via western blot. MSCs immunophenotypic properties were determined by flow cytometry. Osteogenic, adipogenic, and neurogenic differentiation potential were evaluated using western blot, Alizarin red staining, oil red-O staining, and morphological analysis.</div></div><div><h3>Results</h3><div>HG exposure led to a significant decrease in cell viability, with increased apoptosis and autophagy, as indicated by increased levels of cleaved caspase-3, cleaved poly (ADP-ribose) polymerase (PARP), and an elevated microtubule-associated protein 1 light chain 3 beta (LC3B)-II/LC3B-I ratio. However, the immunophenotypic characteristics of DPSCs remained unchanged. DPSCs also demonstrated enhanced osteogenic, adipogenic, and neurogenic differentiation potential by expressing Alizarin red and oil red-O staining, neural-like cell morphology, and several differentiation-related proteins after HG culture stimulation.</div></div><div><h3>Conclusion</h3><div>The present study demonstrated that while HG slightly impairs DPSC viability, it promotes osteogenic, adipogenic, and neurogenic differentiation. Providing valuable insights into the mechanisms by which HG influences various differentiation pathways in DPSCs and establishes a foundation for potential clinical applications of DPSCs in regenerative medicine for diabetic patients.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1731-1738"},"PeriodicalIF":3.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Full-length 16S rRNA sequencing reveals microbial characteristics in supragingival plaque of periodontitis patients 全长度16S rRNA测序揭示牙周炎患者龈上菌斑微生物特征
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-12 DOI: 10.1016/j.jds.2025.03.040
Chih-Chieh Wen , Jerry C.Y. Lin , Eisner Salamanca , Nai-Chia Teng , Yi-Jen Lin , Yi-Fan Wu , Hung-Ming Chang , Wei-Jen Chang
{"title":"Full-length 16S rRNA sequencing reveals microbial characteristics in supragingival plaque of periodontitis patients","authors":"Chih-Chieh Wen ,&nbsp;Jerry C.Y. Lin ,&nbsp;Eisner Salamanca ,&nbsp;Nai-Chia Teng ,&nbsp;Yi-Jen Lin ,&nbsp;Yi-Fan Wu ,&nbsp;Hung-Ming Chang ,&nbsp;Wei-Jen Chang","doi":"10.1016/j.jds.2025.03.040","DOIUrl":"10.1016/j.jds.2025.03.040","url":null,"abstract":"<div><h3>Background/Purpose</h3><div>Periodontitis is a chronic inflammatory disease that disrupts oral microbial homeostasis and contributes to systemic inflammation. While previous studies have focused on subgingival microbiota, the role of supragingival plaque as an early microbial reservoir remains underexplored. Most previous studies have relied on short-read 16S rRNA sequencing but limited detailed classification. This study uses Third-Generation Sequencing (TGS) with full-length 16S rRNA sequencing and DADA2-based error correction to better characterize the supragingival microbiome in periodontitis.</div></div><div><h3>Materials and methods</h3><div>A total of 30 participants (15 periodontitis patients and 15 healthy controls) were recruited. Supragingival plaque samples were collected, and full-length 16S rRNA sequencing was performed using a TGS platform. Sequencing data were processed with DADA2 for error correction and taxonomic classification using the Human Oral Microbiome Database (HOMD), including diversity indices and Principal Component Analysis (PCA), which were conducted to compare microbial compositions between groups.</div></div><div><h3>Results</h3><div>The periodontitis group exhibited significantly higher microbial diversity (<em>P</em> &lt; 0.05) and enrichment of key periodontal pathogens, including <em>Porphyromonas</em>, <em>Prevotella</em>, <em>Fusobacterium</em>, and <em>Treponema</em>. On the other hand, commensal bacteria such as <em>Streptococcus</em> and <em>Neisseria</em> were more abundant in the healthy group. PCA demonstrated distinct clustering patterns, indicating supragingival microbial shifts associated with disease progression.</div></div><div><h3>Conclusion</h3><div>This study provides the high-resolution microbial profiling of supragingival plaque in periodontitis using full-length 16S rRNA sequencing. The findings suggest that supragingival plaque may serve as an early-stage reservoir for periodontal pathogens, which leads to subgingival colonization and disease progression. It highlights the potential of microbial biomarkers in early diagnosis and suggests that targeted microbiome-based therapies could be developed to restore microbial balance in periodontal disease. This method offers greater precision in bacterial identification and future translational potential for personalized periodontal care.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1739-1748"},"PeriodicalIF":3.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the physical and mechanical properties of 3D-printed resin for orthodontic fixed lingual retainers: An in vitro study 正畸固定舌固位体3d打印树脂的物理力学性能评价:体外研究
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-10 DOI: 10.1016/j.jds.2025.03.034
Pyi Phyo Win , Szu-Yu Lai , Daniel De-Shing Chen , Bolormaa Sainbayar , Tzu-Yu Peng , Johnson Hsin-Chung Cheng
{"title":"Evaluation of the physical and mechanical properties of 3D-printed resin for orthodontic fixed lingual retainers: An in vitro study","authors":"Pyi Phyo Win ,&nbsp;Szu-Yu Lai ,&nbsp;Daniel De-Shing Chen ,&nbsp;Bolormaa Sainbayar ,&nbsp;Tzu-Yu Peng ,&nbsp;Johnson Hsin-Chung Cheng","doi":"10.1016/j.jds.2025.03.034","DOIUrl":"10.1016/j.jds.2025.03.034","url":null,"abstract":"<div><h3>Background/Purpose</h3><div>Fixed lingual retainers (FLRs) often experience structural instability, including poor adaptation, and the untwisting of spiral wires, which can lead to unwanted tooth movement. Digital workflows have enhanced the appliance's accuracy. This study evaluated the water sorption (W<sub>sp</sub>), water solubility (W<sub>sl</sub>), staining resistance, and flexural performance of three-dimensionally printed resin (3DR) to determine its optimal design for FLRs.</div></div><div><h3>Materials and methods</h3><div>Cylindrical 3DR specimens were immersed in distilled water and artificial saliva for 7, 14, and 30 days. W<sub>sp</sub> and W<sub>sl</sub> were measured according to ISO 4049 guidelines. Color stability was analyzed after 7 days of immersion in staining solutions using the CIEDE2000 formula. Straight 3DR specimens with various cross-sectional shapes and dimensions underwent a three-point bending test, comparing mechanical properties to multistrand stainless-steel wire (MSW). Statistical analysis was performed using a one-way ANOVA with Tukey's post-hoc test (<em>P</em> &lt; 0.05).</div></div><div><h3>Results</h3><div>W<sub>sp</sub> and W<sub>sl</sub> values remained within ISO standards, with no significant changes over time. Specimens exposed to red wine, grape juice, and curry exhibited color changes exceeding the acceptability threshold of 1.77, while other solutions caused minimal discoloration. Hemi-elliptical cross-sections demonstrated significantly greater flexural strength than round designs. Although all 3DR specimens fractured before reaching the 2.0-mm deflection limit, some designs exhibited comparable bending strength to MSW while maintaining sufficient flexibility for physiological tooth movement.</div></div><div><h3>Conclusion</h3><div>A hemi-elliptical 3DR design (1.0 mm thick and 2.0 mm wide) demonstrated stability in the oral environment, moderate stainability, and mechanical performance comparable to MSW, supporting its feasibility for FLRs.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1723-1730"},"PeriodicalIF":3.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A foreign dentist practicing in Shanghai, China in the late Qing Dynasty: The case of Dr. Sanger's letter notifying the relocation of his dental office in 1895 清末在中国上海执业的外国牙医:1895年桑格医生通知牙科诊所搬迁的信
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-09 DOI: 10.1016/j.jds.2025.03.036
Feng-Chou Cheng , Ling-Hsia Wang , Chun-Pin Chiang
{"title":"A foreign dentist practicing in Shanghai, China in the late Qing Dynasty: The case of Dr. Sanger's letter notifying the relocation of his dental office in 1895","authors":"Feng-Chou Cheng ,&nbsp;Ling-Hsia Wang ,&nbsp;Chun-Pin Chiang","doi":"10.1016/j.jds.2025.03.036","DOIUrl":"10.1016/j.jds.2025.03.036","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1921-1924"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of insertion load on insertion torque value 插入载荷对插入转矩值的影响
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-07 DOI: 10.1016/j.jds.2025.03.029
Po-Yuan Hsueh, Yoko Yamaguchi, Yasutomo Yajima
{"title":"Effect of insertion load on insertion torque value","authors":"Po-Yuan Hsueh,&nbsp;Yoko Yamaguchi,&nbsp;Yasutomo Yajima","doi":"10.1016/j.jds.2025.03.029","DOIUrl":"10.1016/j.jds.2025.03.029","url":null,"abstract":"<div><h3>Background/Purpose</h3><div>This study examined the effect of insertion load on implant primary stability by evaluating the insertion torque and insertion time in various implant designs.</div></div><div><h3>Materials and methods</h3><div>Four implant designs were tested, including one cylindrical implant standard (S), two hybrid implants tapered effect (TE) and bone level (BL), and one conical implant bone level tapered (BLT). Polyurethane bone models of the maxillary posterior region were used. Insertion torque value (ITV) and insertion time, defined as the duration from implant placement initiation to platform alignment, were recorded under two load conditions, the minimum load and a load of 5.0 newton (N). A torque meter was used to capture torque–time curves, and the mean and standard deviation of ITV were calculated. Data were analyzed using a paired t-test (<em>P</em> &lt; 0.05).</div></div><div><h3>Results</h3><div>The minimum insertion load varied by design: implant S required 2.5 N, implants TE and BL each required 2.0 N, and implant BLT required 1.0 N. At minimum load, insertion torque was 8.68 N cm for implant S, 6.64 N cm for implant TE, 12.29 N cm for implant BL, and 29.52 N cm for implant BLT. Under 5.0 N, the values were 8.12, 7.82, 14.89, and 30.53 N cm, respectively. Insertion time decreased by up to 12.52 % from 1.0 N to 5.0 N, with significant differences in implant BLT.</div></div><div><h3>Conclusion</h3><div>Hybrid implants are more sensitive to load variations. Optimizing the insertion load based on implant design can enhance clinical outcomes. The insertion load is a critical but often overlooked factor in primary implant stability.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1861-1868"},"PeriodicalIF":3.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance of ChatGPT in answering the oral pathology questions of various types or subjects from Taiwan National Dental Licensing Examinations
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-05 DOI: 10.1016/j.jds.2025.03.030
Yu-Hsueh Wu , Kai-Yun Tso , Chun-Pin Chiang
{"title":"Performance of ChatGPT in answering the oral pathology questions of various types or subjects from Taiwan National Dental Licensing Examinations","authors":"Yu-Hsueh Wu ,&nbsp;Kai-Yun Tso ,&nbsp;Chun-Pin Chiang","doi":"10.1016/j.jds.2025.03.030","DOIUrl":"10.1016/j.jds.2025.03.030","url":null,"abstract":"<div><h3>Background/purpose</h3><div>ChatGPT, a large language model, can provide an instant and personalized solution in a conversational format. Our study aimed to assess the potential application of ChatGPT-4, ChatGPT-4o without a prompt (ChatGPT-4o-P<sup>-</sup>), and ChatGPT-4o with a prompt (ChatGPT-4o-P<sup>+</sup>) in helping dental students to study oral pathology (OP) by evaluating their performance in answering the OP multiple choice questions (MCQs) of various types or subjects.</div></div><div><h3>Materials and methods</h3><div>A total of 280 OP MCQs were collected from Taiwan National Dental Licensing Examinations. The chatbots of ChatGPT-4, ChatGPT-4o-P<sup>-</sup>, and ChatGPT-4o-P<sup>+</sup> were instructed to answer the OP MCQs of various types and subjects.</div></div><div><h3>Results</h3><div>ChatGPT-4o-P<sup>+</sup> achieved the highest overall accuracy rate (AR) of 90.0 %, slightly outperforming ChatGPT-4o-P<sup>-</sup> (88.6 % AR) and significantly exceeding ChatGPT-4 (79.6 % AR, <em>P</em> &lt; 0.001). There was a significant difference in the AR of odd-one-out questions between ChatGPT-4 (77.2 % AR) and ChatGPT-4o-P<sup>-</sup> (91.3 % AR, <em>P</em> = 0.015) or ChatGPT-4o-P<sup>+</sup> (92.4 % AR, <em>P</em> = 0.008). However, there was no significant difference in the AR among three different models when answering the image-based and case-based questions. Of the 11 different OP subjects of single-disease, all three different models achieved a 100 % AR in three subjects; ChatGPT-4o-P<sup>+</sup> outperformed ChatGPT-4 and ChatGPT-4o-P<sup>-</sup> in other 3 subjects; ChatGPT-4o-P<sup>-</sup> was superior to ChatGPT-4 and ChatGPT-4o-P<sup>+</sup> in another 3 subjects; and ChatGPT-4o-P<sup>-</sup> and ChatGPT-4o-P<sup>+</sup> had equal performance and both were better than ChatGPT-4 in the rest of two subjects.</div></div><div><h3>Conclusion</h3><div>In overall evaluation, ChatGPT-4o-P<sup>+</sup> has better performance than ChatGPT-4o-P<sup>-</sup> and ChatGPT-4 in answering the OP MCQs.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1709-1715"},"PeriodicalIF":3.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impacts of surface characteristics on biological responses and biofilm formation of 3D-printed denture base resins: An in vitro study 3d打印义齿基托树脂表面特性对生物反应和生物膜形成的影响:体外研究
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-04 DOI: 10.1016/j.jds.2025.03.027
Chien-Fu Tseng , Chia-Chih Sung , Ya-Ting Yang , Fen-Ni Chen , Yuichi Mine , Yao-Chang Chiang , Zih-Chan Lin , Mei-Ling Fang , Hsin-Ming Chen , Sang-Heng Kok , Tzu-Yu Peng
{"title":"Impacts of surface characteristics on biological responses and biofilm formation of 3D-printed denture base resins: An in vitro study","authors":"Chien-Fu Tseng ,&nbsp;Chia-Chih Sung ,&nbsp;Ya-Ting Yang ,&nbsp;Fen-Ni Chen ,&nbsp;Yuichi Mine ,&nbsp;Yao-Chang Chiang ,&nbsp;Zih-Chan Lin ,&nbsp;Mei-Ling Fang ,&nbsp;Hsin-Ming Chen ,&nbsp;Sang-Heng Kok ,&nbsp;Tzu-Yu Peng","doi":"10.1016/j.jds.2025.03.027","DOIUrl":"10.1016/j.jds.2025.03.027","url":null,"abstract":"<div><h3>Background/purpose</h3><div>With advancements in digital technology, fully digital workflow for complete denture fabrication using 3D-printed denture base resin (DBR) has gained increasing clinical acceptance in recent years. However, the surface characteristics, biocompatibility, and biofilm formation of 3D-printed DBR materials remain insufficiently understood. Therefore, in this study, we investigated and analyzed these aspects.</div></div><div><h3>Materials and methods</h3><div>Disk-shaped DBR specimens (Ø 2.5 mm, 3 mm thick) were fabricated using packed (PA), milled (ML), and 3D-printed (3D) processes. All specimens were ground with silicon carbide sandpaper (#600) and ultrasonically cleaned. Surface microtopography and sub-micron roughness were analyzed using scanning electron microscopy and atomic force microscopy, while a goniometer was used to measure contact angles to calculate the surface energy. Human gingival fibroblasts and <em>Aggregatibacter actinomycetemcomitans</em> were cultured on the specimens to assess the cytotoxicity and biofilm formation. Statistical analyses were performed with a significance level set to 0.05.</div></div><div><h3>Results</h3><div>Microscopic imaging revealed that the 3D group exhibited a more uniformly distributed texture, while it also had the lowest surface roughness (0.85 μm). Additionally, the PA group had the most hydrophobic surface (82.47°) and the highest surface free energy (46.08 mN/m). Notably, no group showed cytotoxic effects after 72 h of testing. In addition, the 3D group demonstrated the lowest biofilm formation after both 24 h and 72 h of microbial culture.</div></div><div><h3>Conclusion</h3><div>3D-printed DBRs exhibited the lowest surface roughness, maintaining non-cytotoxic and superior resistance to microbial adhesion, suggesting their potential for complete denture fabrication, easy maintenance of oral hygiene, and long-term clinical performance.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1716-1722"},"PeriodicalIF":3.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of 3D-printers and manufacturer-specified post-curing units on the dimensional accuracy, compressive strength, and degree of conversion of resin for fixed dental prostheses 3d打印机和制造商指定的后固化单元对固定义齿的尺寸精度、抗压强度和树脂转化程度的影响
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-04 DOI: 10.1016/j.jds.2025.03.024
Citra Dewi Sahrir , Wei-Shao Lin , Ching-Shuen Wang , Hwai-En Lin , Chin-Wei Wang , Wei-Chun Lin
{"title":"Effects of 3D-printers and manufacturer-specified post-curing units on the dimensional accuracy, compressive strength, and degree of conversion of resin for fixed dental prostheses","authors":"Citra Dewi Sahrir ,&nbsp;Wei-Shao Lin ,&nbsp;Ching-Shuen Wang ,&nbsp;Hwai-En Lin ,&nbsp;Chin-Wei Wang ,&nbsp;Wei-Chun Lin","doi":"10.1016/j.jds.2025.03.024","DOIUrl":"10.1016/j.jds.2025.03.024","url":null,"abstract":"<div><h3>Background/purpose</h3><div>The 3D printer and post-curing unit are important factors in producing the best 3D printed crowns. To explore the effects of different combinations of 3D-printers and manufacturer-specified post-curing units on the dimensional accuracy, compressive strength, and degree of conversion (DC%) of 3D-printable resin for fixed dental prostheses.</div></div><div><h3>Materials and methods</h3><div>Specimens were designed in 2 sizes and additively-manufactured using 2 digital light processing (DLP) 3D-printers (NextDent 5100, ND and PrintinDLP+, PN). The 3D-printed samples were polymerized using 2 different post curing units (LC-3D Print box, N and PrintInCure+, P). Dimensional accuracy was evaluated under an optical microscope, while compressive strength was determined using a universal testing machine. Fourier transform infrared spectroscopy was used to analyze the resin molecular bond characteristics and DC%. Statistical analysis, including ANOVA and Tukey's HSD post-hoc tests (<em>P</em> &lt; 0.05).</div></div><div><h3>Results</h3><div>Significant dimensional variations were observed for both square and rectangular samples (<em>P</em> &lt; 0.001). The ND-P showed the greatest ductility and relatively high maximum stress. The fracture strengths were ND-N: 181.55 ± 8.37 MPa, ND-P: 151.54 ± 2.06 MPa, PN-N: 175.51 ± 12.44 MPa, and PN-P: 127.84 ± 10.10 MPa (<em>P</em> &lt; 0.001). Surface inspection at 200 × magnification revealed subtler fault lines in ND-N and PN-P. FTIR analyses confirmed DC% was highest for ND-N (79.70 ± 1.02%) and PN-N (78.12 ± 0.94%), intermediate for ND-P (73.24 ± 0.89%) and PN-P (71.06 ± 1.67%).</div></div><div><h3>Conclusion</h3><div>Post-curing units had a greater impact on dimensional accuracy, strength, and polymerization than the choice of 3D-printer. Optimal resin properties require careful optimization of post-curing parameters and equipment.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1699-1708"},"PeriodicalIF":3.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Probiotics to reduce microbiota-related dental stains: A potential approach 益生菌减少与微生物有关的牙斑:一种潜在的方法
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-03 DOI: 10.1016/j.jds.2025.03.021
Jing-Jie Yu , Yvonne Hernandez-Kapila , Chin-Wei Wang
{"title":"Probiotics to reduce microbiota-related dental stains: A potential approach","authors":"Jing-Jie Yu ,&nbsp;Yvonne Hernandez-Kapila ,&nbsp;Chin-Wei Wang","doi":"10.1016/j.jds.2025.03.021","DOIUrl":"10.1016/j.jds.2025.03.021","url":null,"abstract":"<div><div>Adult extrinsic black stains on teeth, caused by bacterial colonization, impact aesthetics and confidence. Conventional treatments can be abrasive and have a high recurrence rate. This pilot case study explores probiotics as an adjunctive approach. Direct application of probiotic powder over the black stains of the teeth was carried out prior to routine home care. Results showed black stain removal was possible with tooth brush and dental floss. Saliva and biofilm samples were analyzed via 16S rRNA sequencing. Microbiome revealed a noticeable reduction in <em>Corynebacterium</em>, a key black stain-associated bacterium, with slight shifts in major phyla like <em>Actinobacteriota</em> and <em>Firmicutes</em>. This case study aimed to evaluate the potential of probiotics in reducing black stains on teeth and assess the associated microbiome changes.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1890-1893"},"PeriodicalIF":3.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The overseas expansion of Japanese dentists in the early 20th century: The case of a Japanese immigrant dentist in North China 20世纪初日本牙医的海外扩张:以中国北方一位日本移民牙医为例
IF 3.4 3区 医学
Journal of Dental Sciences Pub Date : 2025-04-02 DOI: 10.1016/j.jds.2025.03.028
Feng-Chou Cheng , Ling-Hsia Wang , Chun-Pin Chiang
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