Journal of Dental Sciences最新文献

{"title":"NCK1 antisense RNA 1 (NCK1-AS1) exerts pro-fibrosis property in oral mucosa through modulation of miR-137/NCK1 axis","authors":"Yu-Feng Huang ,&nbsp;Yu-Lei Wei ,&nbsp;Shih-Min Wang ,&nbsp;Po-Yu Yang ,&nbsp;Pei-Ling Hsieh ,&nbsp;Jung-Chun Yeh ,&nbsp;Yi-Wen Liao ,&nbsp;Cheng-Chia Yu ,&nbsp;Wan-Yin Kuo","doi":"10.1016/j.jds.2024.11.003","DOIUrl":"10.1016/j.jds.2024.11.003","url":null,"abstract":"<div><h3>Background/Purpose</h3><div>Oral submucous fibrosis (OSF) is a premalignant condition of the oral cavity, and its pathogenesis remains largely unknown. A multitude of non-coding RNAs are aberrantly expressed in OSF, and their implication for the development of OSF is a matter meriting investigation.</div></div><div><h3>Materials and methods</h3><div>The functional role of long non-coding RNA NCK1-AS1 in myofibroblast activation of fibrotic buccal mucosal fibroblasts (fBMFs) derived from OSF tissues was assessed. Wound healing, collagen gel contraction and transwell migration assays have been employed to assess the myofibroblast activities. In addition, a luciferase-based reporter assay was used to illustrate the potential mechanism underlying the regulation of NCK1-AS1 in myofibroblast activation.</div></div><div><h3>Results</h3><div>Silencing of NCK1-AS1 markedly downregulated myofibroblast activation and the expression of fibrosis markers in fBMFs. Besides, we demonstrated that NCK1-AS1 directly interacted with microRNA-137 (miR-137) and was negatively correlated with it. Moreover, we found that NCK1 was a target of miR-137 and positively related to NCK1-AS1. Our results demonstrated that NCK1-AS1 may regulate myofibroblast activation by suppressing miR-137 and upregulating NCK1.</div></div><div><h3>Conclusion</h3><div>We showed that NCK1-AS1 acted as a sponge of miR-137 and titrated the suppressive effect of miR-137 on NCK1 to modulate myofibroblast activation in OSF condition.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 632-638"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stress promotes osteogenic and odontogenic differentiation of stem cells from apical papilla via glucose-regulated protein 78-mediated autophagy","authors":"Xiaolan Zhang ,&nbsp;Zhou Wei ,&nbsp;Yunlong Xu","doi":"10.1016/j.jds.2024.05.007","DOIUrl":"10.1016/j.jds.2024.05.007","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Heat stress is essential for improving the efficacy of mesenchymal stem cell (MSC)-based regeneration medicine. However, it is still unclear whether and how heat stress influences the differentiation of stem cells from apical papilla (SCAPs). This research aimed to explore the potential mechanism of glucose-regulated protein 78 (GRP78) in regulating differentiation under heat stress in SCAPs.</div></div><div><h3>Materials and methods</h3><div>The proliferation ability was assessed using the 5-Ethynyl-2’- deoxyuridine (EdU) assay, cell counting kit assay (CCK-8), and flow cytometry (FCM). The osteogenic and odontogenic differentiation capacities were investigated through Western blot, quantitative reverse transcription polymerase chain reaction (qRT-PCR), alkaline phosphatase (ALP) staining and activity assay, alizarin red S (ARS) staining, as well as immunofluorescence staining. Western blot and transmission electron microscopy (TEM) were used to detect autophagy.</div></div><div><h3>Results</h3><div>Heat stress enhanced the osteogenic and odontogenic differentiation of SCAPs, but it did not significantly affect proliferation. Besides, GRP78 has been confirmed to modulate the differentiation induced by heat stress. Autophagy triggered by GRP78 enhanced osteogenic and odontogenic differentiation of SCAPs, while the knockdown of GRP78 or the inhibitor of autophagy suppressed the differentiation.</div></div><div><h3>Conclusion</h3><div>Heat stress induces osteogenic and odontogenic differentiation of SCAPs through GRP78-mediated autophagy.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 487-501"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141057463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleanolic acid inhibits aldo-keto reductase family 1 member B10-induced cancer stemness and avoids cisplatin-based chemotherapy resistance via the Snail signaling pathway in oral squamous cell carcinoma cell lines","authors":"Hui-Hsin Ko ,&nbsp;Han-Yi E. Chou ,&nbsp;Hsin-Han Hou ,&nbsp;Wei-Ting Kuo ,&nbsp;Wei-Wen Liu ,&nbsp;Mark Yen-Ping Kuo ,&nbsp;Shih-Jung Cheng","doi":"10.1016/j.jds.2024.09.018","DOIUrl":"10.1016/j.jds.2024.09.018","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Oral squamous cell carcinoma (OSCC) is a common malignancy often associated with poor prognosis due to chemoresistance. In this study, we investigated whether arecoline, a major alkaloid in betel nuts, can stimulate aldo-keto reductase family 1 member B10 (AKR1B10) levels in OSCC, promoting cancer stemness and leading to resistance to cisplatin (CDDP)-based chemotherapy.</div></div><div><h3>Materials and methods</h3><div>Gain- and Loss- of AKR1B10 functions were analyzed using WB and q-PCR of OSCC cells. Stemness, epithelial mesenchymal transition (EMT) markers, and CDDP drug resistance in overexpressed AKR1B10 were also identified.</div></div><div><h3>Results</h3><div>Upregulated AKR1B10 in OSCC significantly increased cell motility and aggregation. The results also showed that the canonical TGF-β1-Smad3 pathway was involved in arecoline-induced AKR1B10 expression, further increasing cancer stemness with CDDP resistance via the Snail-dependent EMT pathway. Moreover, oleanolic acid (OA) and ROS/RNS (reactive oxygen/nitrogen species) inhibitors effectively reversed AKR1B10-induced CDDP-resistance.</div></div><div><h3>Conclusion</h3><div>Arecoline-induced ROS/RNS to hyper-activate AKR1B10 in tumor sphere cells via the TGF-β1-Smad3 pathway. Furthermore, AKR1B10 enhanced CDDP resistance in OSCC cells via EMT-inducing markers. Finally, Finally, OA may efficiently target CDDP resistance, reverse stemness in OSCC cells, and have the potential as a novel anticancer drug.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 100-108"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immediate orthodontic treatment after regeneration of periodontal intrabony defects: A long-term retrospective study","authors":"Shing-Zeng Dung ,&nbsp;I-Shiang Tzeng ,&nbsp;Cheng-Shan Li","doi":"10.1016/j.jds.2024.06.003","DOIUrl":"10.1016/j.jds.2024.06.003","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Orthodontic movement is often necessary for periodontally compromised patients to enhance esthetics, function, and long-term occlusal stability. However, the impact of orthodontic treatment immediately following the regeneration of intrabony defects on periodontal healing remains a topic of debate. The objective of this long-term case series study was to test the hypothesis that orthodontic treatment performed immediately after regenerative procedures for periodontal intrabony defects did not adversely affect periodontal healing.</div></div><div><h3>Materials and methods</h3><div>This case series study involved nine periodontally compromised subjects with a total of 17 intra-bony defects. Orthodontic brackets were applied immediately before surgery. Flaps were raised, and diseased roots were debrided. All intra-bony defects were filled with alloplastic bone grafts and covered with resorbable membranes, except for one defect treated with Emdogain. All patients initiated orthodontic treatment immediately after periodontal regenerative surgery. Clinical parameters, including probing depth, attachment level, and bone fill, were assessed at baseline and during final maintenance therapy.</div></div><div><h3>Results</h3><div>The mean follow-up duration was 12.8 years. None of the 17 surgically regenerated teeth were lost. The mean reduction in probing depth was 3.94 mm (95% confidence level, 3.19–4.68; <em>P</em> &lt; 0.001), with a mean clinical attachment gain of 3.47 mm (95% confidence level, 2.90–4.03; <em>P</em> &lt; 0.001). The mean radiographic bone fill was 4.89 mm (<em>P</em> &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Based on the findings of this long-term case study, it can be concluded that immediate orthodontic treatment does not adversely affect the maturation process of periodontal regeneration outcomes and can be maintained for more than ten years.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 417-427"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141389817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving osteogenic properties of zirconia ceramic via glow discharge plasma-enhanced deposition of amine organic compound","authors":"Lwin Moe Aung ,&nbsp;Ting-Yi Renn ,&nbsp;Jerry Chin-Yi Lin ,&nbsp;Eisner Salamanca ,&nbsp;Yi-Fan Wu ,&nbsp;Yu-Hwa Pan ,&nbsp;Nai-Chia Teng ,&nbsp;Haw-Ming Huang ,&nbsp;Ying-Sui Sun ,&nbsp;Wei-Jen Chang","doi":"10.1016/j.jds.2024.08.011","DOIUrl":"10.1016/j.jds.2024.08.011","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Osseointegration potential is greatly depended on the interaction between bone cells and dental implant surface. Since zirconia ceramic has a bioinert surface, functionalization of the surface with an organic compound allylamine was conducted to overcome its drawback of minimal interaction with the surrounding bone.</div></div><div><h3>Materials and methods</h3><div>The zirconia surface was initially treated with argon glow discharge plasma (GDP), then combined with amine plasma at three different conditions of 50-W, 75-W and 85-W, to prepare the final samples. The surface characteristics and cell biocompatibility were then evaluated.</div></div><div><h3>Results</h3><div>Surface morphology analysis revealed a bulbous pattern on allylamine-treated sample groups. The aromatic C–H, C–O, N–H, C ˆ C, and C–H stretching and functional groups have been identified. Surface roughness increased, and hydrophilicity improved after surface modification. Cell viability analysis showed the highest result for the allylamine 50-W (A50) group. Alkaline phosphatase (ALP) assay indicated the A50 group had the highest activity, subsequently promoting late-stage mineralization at day 21. The reverse transcription-quantitative polymerase chain reaction (RT-qPCR) data demonstrated a significant upregulation of osteogenic gene expressions from day 1 to day 21.</div></div><div><h3>Conclusion</h3><div>The allylamine-treated surface demonstrates immense enhancement in the surface hydrophilicity as well as in the viability, differentiation, and osteogenic properties of osteoblast-like cells. This makes it a promising candidate for future dental implant applications.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 178-188"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Riboflavin-ultraviolet-A collagen crosslinking treatments in improving dentin bonding and resistance to enzymatic digestion","authors":"Yung-Show Chiang ,&nbsp;Ping-Ju Chen ,&nbsp;Chun-Chan Ting ,&nbsp;Yuh-Ling Chen ,&nbsp;Shu-Fen Chuang","doi":"10.1016/j.jds.2024.09.022","DOIUrl":"10.1016/j.jds.2024.09.022","url":null,"abstract":"<div><h3>Background/purpose</h3><div>The efficacy of riboflavin-ultraviolet-A (RF-UVA) treatment in crosslinking collagen and improving dentin bonding has been proven. However, biodegradation of the hybrid layer may compromise the bonding. The purpose of this study was to evaluate different RF-UVA treatments regarding their ability to preserve dentin bonding from enzymatic digestion.</div></div><div><h3>Materials and methods</h3><div>Collagen subjected to different RF (0.1 %, 1 %)-UVA (1, 2, 5 min) treatments and 5 % glutaraldehyde (GA), without or with enzymatic digestion, were examined by gel electrophoresis (SDS-PAGE). Twenty-five teeth with exposed dentin were primed with one of three RF-UVA treatments (0.1 %RF/1-minUVA, 0.1 %RF/2-minUVA, and 1 %RF/1-minUVA), GA, or distilled water after acid-etching, then restored with an adhesive and a resin composite. After 24-h storage, these teeth were sectioned into microbeams. Half of them received an early microtensile bond strength (μTBS) test, while the other half was stored in enzyme solution for 7 days before testing. Nanoleakage and hybrid layer degradation were examined by TEM.</div></div><div><h3>Results</h3><div>According to SDS-PAGE results, all groups showed the dissipation of intense γ bands of collagen after digestion. For the early bonded specimens and after enzymatic digestions, 0.1 %RF/2-minUVA treated group presented the highest μTBS and none of premature failure. Its TEM images showed less nanoleakage after digestion, which is contributed to the well suspended collagen fibrils and resin infiltration in the hybrid layer.</div></div><div><h3>Conclusion</h3><div>RF-UVA treatment attained collagen crosslinking effects to improve resin-dentin bonding. 0.1 %RF/2-minUVA effectively enhanced dentin bond strength and resistance to enzymatic digestion by optimally expanding dentinal collagen matrix to facilitate hybrid layer formation.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 109-117"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-specific correlations between orbital volume and anthropometric characteristics in Taiwanese adults","authors":"Yu-Chuan Tseng ,&nbsp;Ying Hsu ,&nbsp;Yu-Hung Lai ,&nbsp;Chun-Ming Chen","doi":"10.1016/j.jds.2024.10.021","DOIUrl":"10.1016/j.jds.2024.10.021","url":null,"abstract":"<div><h3>Background/purpose</h3><div>There is no study available addressing the relationship between orbital volume (OV) and skeletal patterns. The purpose of this study was to investigate the correlations between the OV and patient's characteristics (sex, age, height, and skeletal patterns) of Taiwanese adults.</div></div><div><h3>Materials and methods</h3><div>Cone-beam computed tomography images of 94 individuals (men: 47; women: 47) were analyzed to measure their OV and maxillary dimensions. The Student t test was used to compare the OVs of men and women. The correlations between the OV and skeletal patterns (Classes I, II, and III) were investigated through one-way analysis of variance followed by post hoc Bonferroni correction.</div></div><div><h3>Results</h3><div>The mean OV was significantly larger in the men than in the women (25.67 ± 1.89 cm³ vs 22.21 ± 1.23 cm³, respectively). In men with a Class I, II, or III skeletal pattern, the mean OV was 25.50 ± 1.70 cm³, 26.42 ± 2.17 cm³, and 25.14 ± 1.62 cm³, respectively. The mean OV was significantly larger in individuals with a skeletal Class II relationship than in those with a skeletal Class I and Class III relationship. The mean OVs (right OV, left OV and total OV) and maxillary dimensions were significant correlated with height. No significant differences were noted in skeletal patterns in the sex-specific group.</div></div><div><h3>Conclusion</h3><div>Men tend to have a larger OV than do women. The OV and maxillary dimensions were significantly correlated with height. Furthermore, the OV does not vary significantly between sex-specific groups with different skeletal patterns.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 47-53"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the roles of exosomes in salivary gland diseases with an emphasis on primary Sjögren's syndrome","authors":"Xianzhen Cui ,&nbsp;Liu Liu ,&nbsp;Chengchen Duan ,&nbsp;Suning Mao ,&nbsp;Guanru Wang ,&nbsp;Honglin Li ,&nbsp;Cheng Miao ,&nbsp;Yubin Cao","doi":"10.1016/j.jds.2024.10.001","DOIUrl":"10.1016/j.jds.2024.10.001","url":null,"abstract":"<div><div>Salivary gland diseases encompass a broad range of conditions, including autoimmune, inflammatory, obstructive, and neoplastic disorders, significantly impacting oral health and overall well-being. Recent research has highlighted the crucial role of exosomes, small extracellular vesicles, in these diseases. Exosomes mediate intercellular communication by transferring bioactive molecules such as proteins, microRNAs, and lipids, positioning them as potential diagnostic biomarkers and therapeutic agents. In primary Sjögren's syndrome (pSS), exosomes derived from Epstein–Barr virus-infected B cells and activated T cells transfer key microRNAs that impair calcium signaling, contributing to glandular dysfunction. Exosome-based biomarkers like Ro/SSA and La/SSB, found in saliva, serum, and tears, offer non-invasive diagnostic tools for early disease detection. Furthermore, mesenchymal stem cell-derived exosomes show promise in treating pSS by modulating immune responses and promoting tissue repair. While exosomes hold promise for the diagnosis and treatment of other salivary gland diseases, such as radiation-induced xerostomia and sialolithiasis, their application remains limited, necessitating further research to unlock their full diagnostic and therapeutic potential. This review focuses on the role of exosomes in salivary gland diseases, with an emphasis on pSS, and highlights the need for future clinical applications and large-scale trials.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 1-14"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A scientometric study on research trends and characteristics of oral leukoplakia and oral lichen planus","authors":"Wei Liu ,&nbsp;Xuemin Shen ,&nbsp;Zhengyu Shen","doi":"10.1016/j.jds.2024.08.025","DOIUrl":"10.1016/j.jds.2024.08.025","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Oral leukoplakia (OLK) and oral lichen planus (OLP) represent two common oral potentially malignant disorders. It would be interesting to know scientific output and characteristics of studies on OLK and OLP.</div></div><div><h3>Materials and methods</h3><div>This study aimed to investigate and compare scientometric characteristics of articles on OLK and OLP in the Scopus database, with emphasis on the analysis of the keywords that can reflect research directions and topics of concern.</div></div><div><h3>Results</h3><div>A total of 1,191 and 2,288 papers on OLK and OLP were retrieved in the Scopus database, respectively. The total citation count was 31,859 and the <em>h</em> index was 75 for articles on OLK, and the total count was 55,491 and the <em>h</em> index was 93 for articles on OLP. For OLK research, oral cancer-related cancer risk, cancer diagnosis, carcinogenesis, prognosis, tumor marker, e.g. protein P53 and Ki-67, risk assessment, e.g. tongue site, tobacco and alcohol consumption were distinctive frequent keywords. For OLP research, drug efficacy, immunosuppressive agents, e.g. corticosteroid, triamcinolone acetonide, tacrolimus, and glucocorticoids, inflammation-related T lymphocyte, lymphocytic infiltration, cytokines, e.g. gamma interferon, tumor necrosis factor, and interleukin 6, complication, e.g. hepatitis C and diabetes mellitus, and scoring system-related visual analog scale, pain, anxiety, quality of life, depression, and questionnaire were distinctive keywords.</div></div><div><h3>Conclusion</h3><div>This study elucidated the comprehensive identification and recognition of the important and relevant research topics concerned, and encouraged more studies in the field of OLK and OLP to refine management strategies of these diseases.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 672-677"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11763191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soft coral Lobophytum crassum extract inhibits migration and induces apoptosis capabilities in human oral squamous cell carcinoma cells","authors":"Shih-Min Wang ,&nbsp;Mei-Chin Lu ,&nbsp;Yu-Ming Hsu ,&nbsp;Chun-Chung Huang ,&nbsp;Chih-Yuan Fang ,&nbsp;Chuan-Hang Yu ,&nbsp;Pin Ju Chueh ,&nbsp;Cheng-Chia Yu ,&nbsp;Shiao-Pieng Lee","doi":"10.1016/j.jds.2024.06.016","DOIUrl":"10.1016/j.jds.2024.06.016","url":null,"abstract":"<div><h3>Background/purpose</h3><div>The extract of the soft coral <em>Lobophytum crassum</em> has shown an anti-cancer activity in various cancer cells. However, its effect on the oral squamous cell carcinoma cell (OSCC) lines remains unclear. The purpose of this study is to investigate the anti-cancer effects of the extract of <em>L. crassum</em> (C127) on the OSCC cells.</div></div><div><h3>Materials and methods</h3><div>This study evaluated the effects of the soft coral extract of <em>L. crassum</em> (C127) on SAS and Ca9-22 cells, cell viability, migration ability, and apoptosis. Electric cell-substrate impedance sensing (ECIS) was parallelly used on SAS cells to confirm the results.</div></div><div><h3>Results</h3><div>C127 affected cell viability, morphology, and migration ability in both cell lines. In SAS cells, C127 inhibited cell viability in a dose dependent manner (<em>P</em> &lt; 0.001) and induced apoptosis at 10 μg/mL (<em>P</em> &lt; 0.05). In addition, C127 significantly inhibited migration ability on both cell lines in a dose-dependent manner (<em>P</em> &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>This study illustrated the potential of marine-derived compounds of <em>L. crassum</em> on its activity against OSCC cells.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 361-367"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141695975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}