Journal of Dental Sciences最新文献

{"title":"Biomechanical effects of titanium alloy removable partial denture framework designs fabricated by selective laser melting in mandibular Kennedy class II partial edentulism: A finite element study.","authors":"Min-Chieh Chang, Ping-Ching Wu, Wei-Shao Lin, Yung-Chung Chen","doi":"10.1016/j.jds.2025.07.3501","DOIUrl":"10.1016/j.jds.2025.07.3501","url":null,"abstract":"<p><strong>Background/purpose: </strong>The biomechanical effects of framework design and material interactions in mandibular free-end saddle removable partial dentures remain unclear. This study evaluated framework variables using finite element analysis.</p><p><strong>Materials and methods: </strong>A mandibular Kennedy Class II model was digitized to create a cobalt-chromium (CoCr) and sixteen Ti-6Al-4V (Ti64) frameworks varying by major connector dimension, clasp configuration, and lingual reciprocal assembly (LRA). Periodontal ligament (PDL) and mucosa layers were incorporated. Contacts were defined, and materials were assumed homogeneous and isotropic. Vertical and oblique loads were applied, and von Mises stresses were analyzed. Model validation was performed using digital image correlation.</p><p><strong>Results: </strong>Ti64 frameworks reduced overall stress by 21%-43% compared with CoCr. Under vertical loading, PDL stress decreased by 40% and framework stress by 32%-46%. Under oblique loading, direct retainer, PDL, and mucosal stresses increased by 3%, 6%, and 13%, respectively. Framework stress distribution was governed by clasp-LRA interaction, whereas increasing connector rigidity reduced stress magnitude and variability rather than peak stress. Supporting tissue responses were primarily determined by major connector dimension, with LRA and clasp effects being design- and loading-dependent.</p><p><strong>Conclusion: </strong>Ti64 frameworks improved load distribution with limited supporting tissue stress increases under oblique loading. Framework behavior was primarily governed by clasp-LRA interaction, while connector dimension determined supporting tissue response. Increasing major connector dimension attenuated LRA-related stress variations, particularly under oblique loading. Coordinated control of clasp configuration, LRA incorporation, and major connector dimension is required for biomechanical optimization.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1469-1479"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of ChatGPT-4, Gemini, and DeepSeek-V3 in answering the prompt-engineered questions from the Taiwan National Dental Technician Licensing Examination over a three-week period.","authors":"Yi-Pang Lee, Ching-Yi Huang, Andy Sun, Chun-Pin Chiang","doi":"10.1016/j.jds.2025.07.3486","DOIUrl":"10.1016/j.jds.2025.07.3486","url":null,"abstract":"<p><strong>Background/purpose: </strong>Large language models (LLMs) have shown promise in answering professional examination questions. This study evaluated the performance of ChatGPT-4, Gemini, and DeepSeek-V3 in answering prompt-engineered questions selected from the 2023 Taiwan National Dental Technician Licensing Examination (TNDTLE) over a three-week period.</p><p><strong>Materials and methods: </strong>A total of 194 Chinese-language text-based multiple-choice questions were selected from the 2023 TNDTLE. The three LLMs answered these questions using a standardized prompt referring to 17 dental technology textbooks. Accuracy rates (ARs) were recorded at baseline and after one, two, and three weeks. Comparisons of ARs over time, among three LLMs, between basic and clinical subjects, and between Chinese-language questions with and without a prompt were performed by McNemar's or chi-square test, where appropriate.</p><p><strong>Results: </strong>Baseline ARs ranged from 70.1% to 75.3% across the three LLMs. None of the three LLMs demonstrated significant improvement over a three-week period. Overall performance was comparable among models, with only one significant difference observed (Gemini outperforming ChatGPT-4 at one week, <i>P</i> = 0.033). ARs were generally higher for basic subjects than for clinical subjects, although most differences were not statistically significant. The prompt-engineering significantly improved the performance of ChatGPT-4 and Gemini at several time points (<i>P</i> < 0.05), whereas DeepSeek-V3 showed no significant improvement over a three-week period.</p><p><strong>Conclusion: </strong>ChatGPT-4, Gemini, and DeepSeek-V3 achieve moderate accuracy in answering prompt-engineered dental technician examination questions but do not exhibit significant improvement over a three-week period. The prompt-engineering can enhance performance for ChatGPT-4 and Gemini but not for DeepSeek-V3.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1573-1582"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of ChatGPT-4, Gemini, and DeepSeek-V3 on English-translated questions from the Taiwan national dental Technician Licensing examination over a three-week period.","authors":"Yi-Pang Lee, Ching-Yi Huang, Andy Sun, Chun-Pin Chiang","doi":"10.1016/j.jds.2025.07.3495","DOIUrl":"10.1016/j.jds.2025.07.3495","url":null,"abstract":"<p><strong>Background /purpose: </strong>Large language models (LLMs) have shown potential in answering professional examination questions. This study evaluated the performance of ChatGPT-4, Gemini, and DeepSeek-V3 in answering English-translated questions from the 2023 Taiwan National Dental Technician Licensing Examination (TNDTLE) over a three-week period.</p><p><strong>Materials and methods: </strong>A total of 194 English-translated, text-based multiple-choice questions were selected from the 2023 TNDTLE. ChatGPT-4, Gemini, and DeepSeek-V3 were used to answer the same set of English-translated questions at four time points: baseline and one-, two-, and three-week follow-ups. Accuracy rates (ARs) were calculated and compared to evaluate changes over time and differences among the three LLMs, between basic and clinical subjects, and between English-translated and original Chinese-language questions.</p><p><strong>Results: </strong>The baseline ARs were 69.1% for ChatGPT-4, 75.8% for Gemini, and 69.6% for DeepSeek- V3. Among the three LLMs, only ChatGPT-4 demonstrated a statistically significant improvement at the three-week follow-up (<i>P</i> = 0.029). No significant differences were observed among the three LLMs at most time points, except that Gemini achieved a significantly higher AR than Deep-Seek-V3 at the three-week follow-up (78.4% vs. 68.6%, <i>P</i> = 0.039). ARs were generally higher for basic subjects than for clinical subjects. ChatGPT-4 and Gemini achieved significantly higher ARs for English-translated questions than for original Chinese-language questions, whereas DeepSeek- V3 showed no significant language-related difference.</p><p><strong>Conclusion: </strong>ChatGPT-4, Gemini, and DeepSeek-V3 demonstrate moderate capability in answering dental technician examination questions but generally show no significant improvement over a three-week period. Translating Chinese-language questions into English may improve the performance of ChatGPT-4 and Gemini.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1511-1519"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-layer biphasic calcium phosphates/tantalum pentoxide coating on titanium for concurrent biocorrosion resistance and enhanced biocompatibility.","authors":"Ying-Sui Sun, Kai-Yi Lin, Wei-Cheng Chen, Her-Hsiung Huang","doi":"10.1016/j.jds.2025.07.3446","DOIUrl":"10.1016/j.jds.2025.07.3446","url":null,"abstract":"<p><strong>Background/purpose: </strong>Titanium (Ti) dental implants must resist biocorrosion while maintaining biocompatibility. This study developed a dual-layer biphasic calcium phosphates/tantalum pentoxide (BCPs/Ta₂O₅) coating on Ti to improve both biocorrosion resistance and biological performance.</p><p><strong>Materials and methods: </strong>A dense Ta₂O₅ inner layer was fabricated via a hydrolysis -condensation process to act as a corrosion barrier. Subsequently, a porous BCPs outer layer was deposited by electrochemical cathodic deposition to provide a bioactive interface. Surface morphology, functional groups, crystalline structure, wettability, surface free energy, coating thickness and nanostructure, protein adsorption, and in vitro bioactivity were characterized. Biocorrosion behavior was evaluated using polarization curve measurements in simulated blood plasma and artificial saliva with and without fluoride ions. Cellular responses, adhesion, proliferation, and differentiation of human bone marrow mesenchymal stem cells, were assessed. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test.</p><p><strong>Results: </strong>The Ta₂O₅ inner layer significantly improved biocorrosion resistance, evidenced by increased corrosion potential and reduced anodic current density, even under fluoride-containing conditions. The porous BCPs outer layer increased wettability, surface free energy, protein adsorption, and in vitro bioactivity, thereby promoting cell spreading, cytoskeletal organization, and proliferation. The bioactive BCPs coating on Ta₂O₅/Ti surface significantly enhanced alkaline phosphatase activity, suggesting a favorable early osteogenic response, without compromising the corrosion protection provided by the underlying Ta₂O₅ layer.</p><p><strong>Conclusion: </strong>Dual-layer BCPs/Ta₂O₅ coating on Ti surface combines enhanced biocorrosion resistance with osteogenic bioactivity and represents a promising surface modification strategy for Ti dental implants, although studies are required to assess long-term stability and durability.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1449-1458"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective accuracy comparison of marker-based and marker-free registrations using dynamic implant navigation.","authors":"Zhiyi Zhu, Shikai Zhao, Yao Gao, Cui Huang, Yong Xia, Wei-Chun Lin, Chenmin Yao","doi":"10.1016/j.jds.2025.07.3559","DOIUrl":"10.1016/j.jds.2025.07.3559","url":null,"abstract":"<p><strong>Background/purpose: </strong>The choice between marker-based and marker-free registration in dynamic computer-assisted implant surgery (d-CAIS) presents a trade-off between potential accuracy and workflow efficiency. This study sought to directly compare these two methods by assessing their impact on implant placement precision and overall surgical time, to inform clinical decision-making.</p><p><strong>Materials and methods: </strong>A retrospective analysis was conducted involving 48 implants placed using d-CAIS, divided into two groups: marker-based and marker-free. Implant placement accuracy was assessed by comparing actual and planned positions using pre- and post-operative cone beam computed tomography scans. Angular and linear deviations at the implant platform and apex were recorded, along with preparation and registration times.</p><p><strong>Results: </strong>The marker-based group exhibited significantly lower linear deviation compared to the marker-free group, particularly at both the platform and apex. However, the two methods showed no significant difference in angular deviation. The marker-free registration method demonstrated a shorter preparation time but a slightly longer registration time, with no significant difference in overall implant positioning accuracy. The marker-free group showed higher linear deviation, particularly in horizontal measurements at the implant platform and apex.</p><p><strong>Conclusion: </strong>This study highlights that marker-based registration offers superior precision, especially in linear deviation, during dynamic computer-assisted implant surgery. In contrast, the marker-free approach provides greater efficiency in preparation time, despite requiring slightly longer registration times. By eliminating the need for additional hardware, the marker-free method presents a viable alternative in specific clinical situations. These findings contribute valuable insights into optimizing surgical workflows and enhancing clinical outcomes in implant dentistry.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1904-1913"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of horizontal dimensional malocclusions on smile changes in frontal, lateral, and oblique views before and after orthodontic treatment.","authors":"Johnson Hsin-Chung Cheng, Sarah Jia-Min Lin, Yuan-Che Hsu, Tsendsuren Khurelochir","doi":"10.1016/j.jds.2025.07.3562","DOIUrl":"10.1016/j.jds.2025.07.3562","url":null,"abstract":"<p><strong>Background/purpose: </strong>Smile esthetics are an important aspect of orthodontic treatment; however, most studies have focused on frontal views. This study examines how horizontal skeletal and dental changes impact smiles from frontal, lateral, and oblique views before and after treatment.</p><p><strong>Materials and methods: </strong>This retrospective study analyzed lateral cephalometric X-rays from orthodontic patients grouped by ANB (I: ≤0°, II: 0-4°, III: ≥4°) and overjet (A: ≤0 mm, B: 0-4 mm, C: 4 ≥ mm). A total of 20, 22, and 22 smile parameters were measured in frontal, lateral, and oblique images. ANOVA and Scheffé tests assessed associations between ANB, OJ, and smile parameters. AI machine learning ranked influencing factors.</p><p><strong>Results: </strong>After treatment, group A showed the greatest maxillary incisor display increase in the frontal view. In the oblique view, only smile length and visible maxillary dental length significantly differed among ANB groups. Smile changes were most prominent in the lateral view. In group I, upper lip landmarks moved upward; in group III, they moved downward. AI machine learning ranked upper maxillary incisor display as the key factor in the frontal view and visible maxillary dental length in lateral and oblique views.</p><p><strong>Conclusion: </strong>Lateral views show the most smile changes post-orthodontic treatment. ANB influences smile changes more than OJ in lateral and oblique views, while OJ dominates in the frontal view. Class III cases show the most significant changes. Upper incisor display is key in the frontal view, while maxillary dental length dominates laterally and obliquely. Including lateral and oblique data can enhance orthodontic diagnosis accuracy.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1933-1941"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative cone-beam computed tomography assessment of anterior loop safety margins using canine-guided osteotomy designs for chin bone harvesting.","authors":"Han-Sheng Chen, Kun-Jung Hsu, Hsiu-Yueh Liu, Hui-Na Lee, Chun-Ming Chen","doi":"10.1016/j.jds.2025.07.3491","DOIUrl":"10.1016/j.jds.2025.07.3491","url":null,"abstract":"<p><strong>Background: </strong>/purpose: Chin bone harvesting is a common intraoral grafting procedure but carries a risk of neurosensory complications involving the mental foramen (MF), anterior loop (AL), and mandibular incisive canal (MIC). Present study quantitatively evaluated whether the mandibular canine can serve as a safer and more reproducible landmark for chin bone harvesting.</p><p><strong>Materials and methods: </strong>Cone-beam computed tomography (CBCT) scans from 40 adults were analyzed. The mandibular canine distal angle (CDA) and canine long axis (CLA) were used as reference lines. Measurements included AL-MF length, distances from the CLA to the MF and AL, interMF distance (IntMF), interAL distance (IntAL), interCLA distance (IntCLA), and the vertical relationship between the canine apex and the MIC. Sex- and side-based comparisons were performed, and correlations with age and variables were assessed. Probability modeling estimated the likelihood of AL or MIC encounter under various osteotomy scenarios.</p><p><strong>Results: </strong>The mean AL-MF length was 1.76 ± 0.96 mm, with no significant sex or side differences. Canine-referenced measurements showed consistent horizontal safety margins, maintaining ≥2 mm from the AL in all cases. At 3 mm posterior to the CLA, the probability of avoiding AL injury exceeded 96.25%. Vertically, the MIC was often close to the canine apex, and deeper osteotomies increased risk. ANB angle demonstrated significant positive correlations with CLA-MF, CLA-AL, IntMF, and IntAL.</p><p><strong>Conclusion: </strong>The mandibular canine is a reliable landmark for horizontal osteotomy planning. This approach improves reproducibility and may reduce nerve exposure. Conservative depth control and cortical-only harvesting are recommended to minimize MIC-related complications.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1542-1550"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Daisaikoto</i> restores mitochondrial function and suppresses inflammation induced by periodontal bacteria-derived lipopolysaccharides.","authors":"Suzuka Tozawa, Kazuo Tomita, Kento Igarashi, Yoshikazu Kuwahara, Junichi Kitanaka, Nobue Kitanaka, Mehryar Habibi Roudkenar, Amaneh Mohammadi Roushandeh, Akihiro Kurimasa, Tomoaki Sato","doi":"10.1016/j.jds.2025.07.3544","DOIUrl":"10.1016/j.jds.2025.07.3544","url":null,"abstract":"<p><strong>Background/purpose: </strong>Periodontal disease affects approximately half of the world's population and is the leading cause of tooth loss. This study aimed to identify effective drug candidates for mitigating oral inflammation induced by <i>Porphyromonas gingivalis</i> lipopolysaccharide (<i>P. g</i> LPS), a major virulence factor of periodontal pathogens. We investigated <i>daisaikoto</i> (DST), known for its anti-inflammatory properties in various tissues, and compared its efficacy with <i>shosaikoto</i> (SSK) and <i>hainosankyuto</i> (HNST), both reported to have beneficial effects against periodontitis.</p><p><strong>Materials and methods: </strong>Human periodontal ligament fibroblasts were treated with DST, SSK, HNST, and their active components before and after <i>P. g</i> LPS stimulation. Anti-inflammatory effects were evaluated by quantifying <i>IL-6</i>, <i>COX2</i>, and <i>PGES2</i> gene expression using qPCR. Mitochondrial function was assessed via measurements of mitochondrial membrane potential (Ψm), Fe²⁺ content (mtFe²⁺), and reactive oxygen species (mtROS). In addition, ATP production and glycolytic gene expression were analyzed.</p><p><strong>Results: </strong>DST markedly suppressed <i>IL-6</i> and <i>PGES2</i> expression more effectively than SSK and inhibited <i>IL-6</i> more strongly than HNST. <i>P. g</i> LPS elevated mtROS and mtFe²⁺ levels while reducing Ψm; these mitochondrial impairments were reversed exclusively by DST. Moreover, DST restored ATP production diminished by <i>P. g</i> LPS exposure.</p><p><strong>Conclusion: </strong>DST exhibits superior anti-inflammatory activity compared with SSK and HNST and uniquely reverses mitochondrial dysfunction caused by <i>P. g</i> LPS. Given the association between mitochondrial dysfunction, diabetes, and periodontal disease progression, DST emerges as a promising therapeutic candidate for periodontitis.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1764-1773"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> characterization of tooth-derived and dentin-derived graft particles: Comparative analysis of particle size, mineral composition, and cytotoxicity.","authors":"Vinh Quang Luu, Nhan Duc Le, Bich-Ly Thi Nguyen, Son Hoang Le","doi":"10.1016/j.jds.2025.07.3540","DOIUrl":"10.1016/j.jds.2025.07.3540","url":null,"abstract":"<p><strong>Background/purpose: </strong>Characteristics of grafting material influence its osteoconductive and osteoinductive properties. This study aimed to compare tooth-derived particles (TDPs) with dentin-derived particles (DDPs) with respect to particle size, demineralization behavior, elemental composition, and cytotoxicity.</p><p><strong>Materials and methods: </strong>TDP materials and DDP materials were cleaned, air-dried, ground using a Smart Dentin Grinder (KometaBio, Bergen, NJ, United States), and sorted by particle size. Both materials were demineralized with 10 % ethylenediaminetetraacetic acid (EDTA) at various time points and analyzed by scanning electron microscopy and energy-dispersive X-ray spectroscopy to determine the structural changes and the time required to reach 30 % residual Ca. Samples that achieved this level were subsequently evaluated for cytotoxicity according to ISO 10993-12:2021.</p><p><strong>Results: </strong>Particle size distribution was significantly different between TDPs and DDPs. The proportion of particles suitable for grafting was higher in DDPs (69.12 %) than in TDPs (55.98 %). Although the percentages of Ca and P during demineralization were slightly higher in TDPs than in DDPs, the differences were not significant. Both Ca and P contents, as well as the Ca/P ratio, decreased significantly with increasing demineralization time. Samples from both groups, adjusted to approximately 30 % residual Ca, were non-cytotoxic and met the ISO safety criteria for biological experimentation.</p><p><strong>Conclusion: </strong>DDPs have a more favorable size distribution than TDPs, requiring 17-20 min of 10 % EDTA to reach optimal 70 % Ca removal. The resulting materials are non-cytotoxic, supporting their safe and effective use as autogenous grafts.</p>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"1723-1731"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Er:YAG laser-assisted connective tissue wall technique for periodontal regeneration in a soft tissue recession combined with a furcation-involved class II subclass B molar under a microscope: A case report.","authors":"Shao-Yu Chang, Yoichi Taniguchi, Akira Aoki, Taichen Lin","doi":"10.1016/j.jds.2025.07.3457","DOIUrl":"10.1016/j.jds.2025.07.3457","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 3","pages":"2023-2025"},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}