Journal of Clinical Laboratory Analysis最新文献

{"title":"Levels of IL-1β, MMP-8, and MMP-9 in the Saliva of Subjects With Periodontitis: A Systematic Review and Meta-Analysis","authors":"Mario Alberto Alarcón-Sánchez,&nbsp;Ruth Rodríguez-Montaño,&nbsp;Seyed Ali Mosaddad,&nbsp;Artak Heboyan","doi":"10.1002/jcla.70040","DOIUrl":"10.1002/jcla.70040","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objective</h3>\u0000 \u0000 <p>Proinflammatory cytokines and enzymes responsible for tissue destruction are important in the development of periodontitis. This study compared salivary concentrations of interleukin-1 beta (IL-1β), matrix metalloproteinases (MMP-8), and (MMP-9) in individuals with and without periodontitis to evaluate their diagnostic utility as potential biomarkers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A comprehensive search was performed across PubMed, Scopus, ScienceDirect, and Google Scholar, supplemented by manual searches in relevant journals up to January 2024. Eligibility criteria focused on human studies with defined diagnostic criteria for periodontitis and saliva samples analyzed for IL-1β, MMP-8, and MMP-9. Data were extracted to compare salivary levels of these markers between periodontitis patients and healthy controls. The Joanna Briggs Institute tool was used to evaluate the risk of bias and quality of the included studies. Statistical analysis employed a random effects model to calculate standardized mean differences and assess heterogeneity and publication bias.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The search yielded 122 articles, with 27 meeting the inclusion criteria. Fifteen percent of these studies presented a moderate risk of bias, while the remaining 85% exhibited a low risk of bias. The meta-analyses indicated significantly higher levels of IL-1β, MMP-8, and MMP-9 in the saliva of subjects with periodontitis compared to healthy individuals: IL-1β: Standardized Mean Difference (SMD) = 163.29 (95% CI = 104.64–221.95), <i>p</i> &lt; 0.001; MMP-8: SMD = 282.22 (95% CI = 209.68–354.77), <i>p</i> &lt; 0.001; MMP-9: SMD = 311.85 (95% CI = 179.64–444.05), <i>p</i> &lt; 0.001.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Elevated salivary levels of IL-1β, MMP-8, and MMP-9 are linked to periodontitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Multiplexed qPCR Kit for the Detection of Bloodstream Infection","authors":"Kai Niu,&nbsp;Yiping Wang,&nbsp;Zhiyu Pang,&nbsp;Miaomiao Niu,&nbsp;Zhitong Sun,&nbsp;Jennie Luo,&nbsp;Chaoqun Xia,&nbsp;Yanqiao Qian,&nbsp;Zhi Xu,&nbsp;Yong Luo,&nbsp;Yong Wu,&nbsp;Dijun Zhang,&nbsp;Jiang Cao","doi":"10.1002/jcla.70037","DOIUrl":"10.1002/jcla.70037","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Bloodstream infection (BSI) is associated with high morbidity and mortality worldwide. Currently, BSI diagnosis relies on a time-consuming blood culture method, which usually takes 2 or more days to identify the causative pathogens. Cell-free DNA (cfDNA) refers to those small nucleic acid fragments residing in plasma and other body fluids, which have been used to detect cancer, organ transplantation injury, and pathogenic infections. A new multiplexed fluorescent quantitative PCR kit aiming at plasma microbial cfDNA was developed in this study. The kit contains multiple panels, and each panel covers multiple pathogens, including <i>E. coli</i>, <i>K. pneumoniae</i>, <i>A. baumannii</i>, <i>H. influenzae</i>, <i>P. aeruginosa</i>, <i>E. faecalis</i>, <i>E. faecium</i>, <i>S. aureus</i>, and <i>S. epidermidis.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>PCR primers and probes were designed based on effective bacterial sequence segments, which were obtained from the analysis of next-generation sequencing results of plasma samples of patients with bloodstream infections. Bioinformatics analyses and experimental evidence were used to test the conservativeness and specificity of the primers and probes. The lower detection limit of the kit was determined under optimal reaction conditions. Clinical samples were used to test the accuracy of the kit's detection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The lower detection limit of the kit has reached ≤ 12 copies per reaction. Clinical samples testing results showed a 90.48% consistency between the kit and blood culture.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The kit provides a rapid, accurate, and reliable method for diagnosing bloodstream infections. This can quickly provide an etiological basis for clinical diagnosis and further treatment, potentially improving patient outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Healthcare Professionals' Knowledge Regarding Nontuberculosis Mycobacterial Infections at Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia, 2024: A Cross-Sectional Study","authors":"Teklehaimanot Kiros,&nbsp;Mulat Erkihun,&nbsp;Bekele Sharew,&nbsp;Andargachew Almaw,&nbsp;Ayenew Assefa,&nbsp;Tegenaw Tiruneh,&nbsp;Birhanu Getie,&nbsp;Yenealem Solomon,&nbsp;Shewaneh Damtie","doi":"10.1002/jcla.70035","DOIUrl":"10.1002/jcla.70035","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Nontuberculosis mycobacteria (NTM) are environmental pathogens that can cause pulmonary infections, especially among the immunocompromised population. There is limited research on healthcare professionals' knowledge of NTM infections in Ethiopia. This study aimed to evaluate healthcare professionals' knowledge about NTM infections at Debre Tabor Comprehensive Specialized Hospital (DTCSH).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A cross-sectional study was conducted from March 5 to July 30, 2024 at DTCSH to evaluate the knowledge of 292 healthcare professionals on NTM infections. A semi-structured questionnaire was used for data collection, and the data were analyzed using SPSS version 25. The study's findings were presented through texts, tables, and figures.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of all the participants, 160 (54.8%) were male, and the majority 153 (52.4%) were 36–45 years old. Nurses made up the largest professional group, 127 (43.5%). Approximately 59% of healthcare professionals showed good knowledge of NTM infections. In this study, a higher percentage of female [92 (69.7%)] participants demonstrated good knowledge compared to males [83 out of 160 males (51.9%)]. Similarly, 35 doctors (89.7%) and 80 nurses (63%) demonstrated favorable knowledge. Only 52.4% could distinguish between NTM and <i>Mycobacterium tuberculosis</i> infections. Notably, 77.4% recognized that NTM infections are more common in immunocompromised individuals, yet only 20.5% knew that NTM species are not typically treated with anti-tuberculosis drugs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The knowledge gaps among healthcare professionals regarding NTM infections necessitate focused educational initiatives to improve their ability to manage these infections. The focused training is essential for enhancing the management and identification of NTM infections.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotype Detection and Drug Resistance Analysis of Carbapenem-Resistant Gram-Negative Bacilli","authors":"Xiao-min Liu,&nbsp;Ya-xian Wu,&nbsp;Xiao-hui Li,&nbsp;Jia-wen Shang,&nbsp;Wen-na Xu,&nbsp;Nan-song Xu,&nbsp;Qiu-yao Zeng,&nbsp;Xin Zheng","doi":"10.1002/jcla.70038","DOIUrl":"https://doi.org/10.1002/jcla.70038","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The increasing prevalence of infections caused by carbapenem-resistant organisms (CRO) represents a global health issue. Therefore, a rapid and accurate method for detecting these microbes in any clinical microbiology laboratory is crucial for the prevention and control of their transmission, as well as for clinical treatment. This study aimed to evaluate the phenotypic detection methods, PBA-EDTA, mCIM, PCR, and immunocolloidal gold kit for CRO.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We collected 99 samples from the inpatients in Sun Yat-Sen University Cancer Center (SYSUCC) from March 2019 to February 2022 and classified the drug resistance and genotype of various strains by various enzyme-type experiments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Out of 99 multidrug-resistant Gram-negative bacilli resistant to carbapenems, 58 (58.59%) were identified as carbapenemase-positive using the mCIM test. The carbapenemase genotypes included 19 NDM strains, 4 KPC strains, 1 IMP strain, and 5 OXA-23 strains. Enzyme detection revealed 21 strains positive for metallo-β-lactamase, 50 for serine-β-lactamase, and 2 positive for both, with a total positive rate of 73.74%. 26 strains were negative for enzyme detection, and mCIM showed limited effectiveness in detecting strains coproducing NDM and KPC. The immunocolloidal gold assay had a sensitivity of 96.9% and specificity of 98.5%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study used an immunocolloidal gold kit for carbapenemase detection, providing results within 15 min. This cost-effective method can quickly assist in identifying carbapenemase genotypes and holds potential as a new rapid detection and diagnostic tool for CRE in clinical and lab settings.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 9","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Enzyme-Linked Immunosorbent Assay and Lateral Flow Assay to Measure Thyroid-Stimulating Hormone and Free T4 in Human Serum","authors":"Rolando Advíncula-Espino,&nbsp;Jair Li,&nbsp;Jaime Rosales-Rimache","doi":"10.1002/jcla.70032","DOIUrl":"10.1002/jcla.70032","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Thyroid disorders affect millions of people around the world, mainly women. Measurement of hormones is critical in their identification, treatment, and monitoring; Therefore, the methods must be as reliable and valid as possible.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We designed a cross-sectional study to compare thyroid-stimulating hormone (TSH) and free tetraiodothyronine (fT4) concentrations using ELISA (Enzyme-Linked Immunosorbent Assay) and LFA (Lateral Flow Assay) in human serum samples. We obtained 96 serum samples that were evaluated with a commercial kit for ELISA (Accubind Monobite, Lake Forest, California, US) and LFA (Micropofit Fluorecare, Guangdong, Shenzhen, China). We used the Wilcoxon nonparametric test, Bland–Altman, and Passing-Bablok regression to compare TSH and fT4 concentrations obtained by ELISA and LFA.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The median TSH concentrations obtained by ELISA and LFA were 1.92 and 2.11 μIU/mL, and for fT4 were 1.14 and 1.10 ng/dL. On the other hand, the Spearman's rho between ELISA and LFA for TSH was 0.845. The TSH concentrations between ELISA and LFA had significant differences (<i>p</i> &lt; 0.05). About fT4, the Spearman's rho between methods was 0.348. The bias for TSH was −0.315, and for fT4 was −0.013. The Deming regression for TSH (<i>p</i> = 0.309) and fT4 (=0.938) shows that the levels obtained by both methods do not present significant differences, and the Passing-Bablok regression identifies significant bias between both methods, especially for fT4, in the range of concentrations studied.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>TSH measurement by LFA may be a viable alternative to evaluate thyroid diseases, but fT4 by LFA is not precise and presents a high bias.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Role of T-Cell Metabolism in Modulating Immunotherapy Efficacy for Non-Small Cell Lung Cancer Based on Clustering.","authors":"Hongzhe Guo, Liangyu Zhang, Hu Tang, Peiwen Liu, Bin Hu, Yue Gong, Rui Hou, Ziheng Wu","doi":"10.1002/jcla.25020","DOIUrl":"https://doi.org/10.1002/jcla.25020","url":null,"abstract":"<p><strong>Background: </strong>Immunotherapy, especially immune checkpoint blockade (ICB) therapy, has demonstrated noteworthy advancements in the realm of non-small cell lung cancer (NSCLC). However, the efficacy of ICB therapy is limited to a small subset of patients with NSCLC, and the underlying mechanisms remain poorly understood.</p><p><strong>Study design and discoveries: </strong>In this study, we conducted a comprehensive investigation of the metabolic profiles of infiltrating T cells in NSCLC tumors and revealed the metabolic heterogeneity, which associated with the prognosis of ICB therapy, in three T-cell subtypes. After metabolic clustering, we split these metabolic clusters into two groups: Nonresponse-associated (NR) clusters that enriched with cells from nonresponders, and response-associated (R) clusters that not belonging to NR clusters. Then, we elucidated their metabolic differences and specific functions. Notably, we discovered HSPA1A was significantly downregulated in NR clusters of all three T-cell subtypes. In addition, leveraging single-cell T-cell receptor sequencing data and pseudotime series analysis, we revealed the reciprocal interconversion between R and NR metabolic clusters within the same T-cell clone. This suggests a potential metabolic reprogramming capability of T cells. Furthermore, through the analysis of intercellular communication, we identified the specific intercellular signaling in the R clusters, which might promote the activation and regulation of signal transduction pathways that affect the prognosis of ICB therapy.</p><p><strong>Conclusion: </strong>In conclusion, our study offers substantial insights into the mechanisms of relationships between T-cell metabolisms and ICB therapy outcomes, shedding light on the mechanism of immunotherapy efficacy in patients with NSCLC. Such investigations will contribute to overcoming treatment resistance.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25020"},"PeriodicalIF":2.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Chromosomal Karyotype Analysis Combined With Chromosomal Microarray Analysis in the Amniotic Fluid of Advanced Maternal Age","authors":"Caihong Liu,&nbsp;Yan Lu,&nbsp;Baohui Zhang,&nbsp;Liucui Yu,&nbsp;Jing He,&nbsp;Yan Ji","doi":"10.1002/jcla.70020","DOIUrl":"https://doi.org/10.1002/jcla.70020","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Objective&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;To explore the application and value of chromosomal karyotype analysis combined with Chromosomal Microarray Analysis (CMA) in the amniotic fluid of advanced maternal age.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;A total of 817 advanced maternal age (AMA) who underwent amniocentesis at the Prenatal Diagnosis Center of Huizhou Central People's Hospital between January 2018 and July 2024 were enrolled in this study. The women were grouped based on different age ranges and prenatal diagnosis factors. These groups were used to compare the detection rates and differences between chromosomal karyotype analysis and CMA.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Result&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The overall chromosomal abnormality rates detected by karyotype analysis in the 35–39 years age group and the ≥ 40 years age group were 8.81% and 13.79%, respectively, with a statistically significant difference (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). For CMA, the overall abnormality rates in the same age groups were 10.79% and 15.33%, respectively, but the difference was not statistically significant (&lt;i&gt;p&lt;/i&gt; &gt; 0.05). The non-solely advanced-age group (those with additional factors beyond just advanced age) had higher overall chromosomal abnormality rates, aneuploidy rates, structural abnormality rates, and mosaicism rates compared to the solely advanced-age group, with statistically significant differences (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). Additionally, the non-solely advanced-age group had a higher overall abnormality rate detected by CMA compared to the solely advanced-age group, with a statistically significant difference (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). However, there were no statistically significant differences between the two groups in terms of the detection of pathogenic, likely pathogenic, and variants of uncertain significance (&lt;i&gt;p&lt;/i&gt; &gt; 0.05). In this study, a total of 68 cases were identified where the results of karyotype analysis and CMA were inconsistent.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusion&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The overall abnormal rate of chromosomal karyotype analysis increases with maternal age, while the overall abnormal rate of CMA shows no significant correlation with maternal age. The abnormal rates are significantly higher in AMA with additional factors. The combination of chromosomal karyotype analysis and CMA can validate and complement each other, thereby improving the detection rates of chromosomal abnormalities in amniotic fluid samples of AMA. This provides a diagnostic basis for subsequent pregnancy choices, which effectively reduces the birth of fetuses with ch","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 9","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating Exosomal Proteins as New Diagnostic Biomarkers for Colorectal Cancer (EXOSCOL01): A Pilot Case–Controlled Study Focusing on MMP14 Potential","authors":"Mickaël Barhoum,&nbsp;Sylvie Brassart-Pasco,&nbsp;Aurélie Dupont-Deshorgue,&nbsp;Aurore Thierry,&nbsp;Lukshe Kanagaratnam,&nbsp;Bertrand Brassart,&nbsp;Fidy Ramaholimihaso,&nbsp;Damien Botsen,&nbsp;Claire Carlier,&nbsp;Mathias Brugel,&nbsp;Marine Perrier,&nbsp;Laurent Ramont,&nbsp;Olivier Bouché","doi":"10.1002/jcla.70016","DOIUrl":"https://doi.org/10.1002/jcla.70016","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Colorectal cancer (CRC) is the second leading cause of cancer-related death worldwide. The French CRC screening campaign is based on fecal immunochemical tests (FIT), confirmed by colonoscopy, an invasive procedure with a poor participation rate. This study aimed to compare the expression of circulating exosomal proteins (MMP14, β1-Integrin subunit, β3-Integrin subunit, and α1(I) Collagen chain) in patients with CRC or adenomas.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 71 patients were recruited, including 24 controls (normal colonoscopy), 11 patients with adenoma, and 36 with CRC. Plasmatic exosomal protein expression was measured by western blot analysis and reported to either protein or exosome content.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The three groups were comparable regarding clinical characteristics. A significant difference was observed for MMP14 relative expression (<i>p</i> = 0.0007), MMP14 expression reported to exosomal protein content (<i>p</i> = 0.0003), and MMP14 expression reported to exosome content (<i>p</i> = 0.0005). These three parameters were significantly higher in patients with adenoma vs. control patients (<i>p</i> = 0.0013, <i>p</i> = 0.0004, and <i>p</i> = 0.0003, respectively). Only MMP14 relative intensity was significantly higher in the CRC group vs. the control group (<i>p</i> = 0.0018).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Exosomal MMP14 is a promising early diagnostic biomarker for CRC and adenoma. These preliminary results warrant confirmation in larger studies using quantitative measurements such as ELISA or flow cytometry.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 9","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Large-Scale Retrospective Study of Serum Des-Gamma-Carboxy Prothrombin as a Diagnostic Marker of HCC: Effect of Liver Function on Specificity","authors":"Hongying Bu,&nbsp;Weijia Luo,&nbsp;Wenli Tao,&nbsp;Chen Dong,&nbsp;Meifang Wang,&nbsp;Xu Ye,&nbsp;Xi Zeng,&nbsp;Boqing Wang,&nbsp;Chang Liu,&nbsp;Qi Yu,&nbsp;Deliang Cao,&nbsp;Hongyu Deng,&nbsp;Yuemin Nan","doi":"10.1002/jcla.70025","DOIUrl":"10.1002/jcla.70025","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>This retrospective multicenter study is aimed at evaluating the diagnostic accuracy and influence factors of serum des-gamma-carboxy prothrombin (DCP) as a diagnostic biomarker of hepatocellular carcinoma (HCC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Clinical data were collected from 4555 subjects with DCP tests, composed of primary liver cancer (PLC), metastatic liver cancer (MLC), chronic hepatitis (CH), liver cirrhosis (LC), benign liver diseases (BLD), biliary tract diseases (BTD), non-liver cancers (NLC), and non-liver benign diseases (NLBD). The clinical data collected included medical history, treatment records, various serum tests, and imaging examination.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Serum DCP was measured with Abbott agents in each center. In HCC, serum DCP concentration was at 9086.00 ± 366.10 mAU/mL, higher than that in other diseases (<i>p</i> &lt; 0.05). At 40.00 mAU/mL recommended by instruction, positive rates of serum DCP were at 85.11% in HCC, 30.12% in intrahepatic cholangiocellular carcinoma (ICC), 31.65% in MLC, 13.95% in BLD, 18.14% in CH, 27.87% in LC, 15.75% in BTD, 35.29% in NLC, and 20.00% in NLBD. In this study, the diagnostic specificity of serum DCP in HCC was affected by liver function. In HCC, serum AFP concentrations also increased compared to non-HCC diseases (<i>p</i> &lt; 0.05), but specificity varied with agents from different providers. Serum DCP decreased after the surgical removal of HCC, but remained elusive in systemic treatment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Serum DCP may serve as an optimal biomarker for the diagnosis of HCC, but its accuracy appears influenced by liver function; attention needs to be paid to the liver function of patients for false positivity.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-Laboratory and Inter-Laboratory Variations Analysis for HbA1c Assays in China: Using Internal Quality Control and External Quality Assessment Data","authors":"Yongyong Lou,&nbsp;Zhiming Shan,&nbsp;Qian Chen,&nbsp;Fengfeng Kang","doi":"10.1002/jcla.70036","DOIUrl":"https://doi.org/10.1002/jcla.70036","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>With the worldwide increase of diabetes mellitus prevalence, ensuring the performance of HbA1c assays is essential. Internal quality control (IQC) and external quality assessment (EQA) serve as critical components of quality assurance systems and provide comprehensive performance assessment. We aimed to evaluate the intra-laboratory and inter-laboratory variations of HbA1c assays using EQA and IQC data.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 326 laboratories continuously participating in the HbA1c EQA program from 2020 to 2023 were included, of which 168 laboratories reported IQC data voluntarily. Acceptance rates and bias were evaluated at three levels: per sample, per year, and per manufacturer. Intra-laboratory and inter-laboratory variations were assessed according to biological variation (BV) criteria and clinical guidelines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean acceptance rates for 20 EQA samples were 48.5%, 77.8%, 86.7% within optimum, desirable, minimum BV criteria. Annual average acceptance rates increased from 91.8% to 96.9% based on EQA criterion. The absolute manufacturer-specific bias varied from 0.02% to 4.1%. By 2023, the overall inter-laboratory variation significantly decreased to 2.1%–2.6%. The median intra-laboratory variations reduced from 1.6% to 1.4% at the low QC level and from 1.2% to 1.0% at the high QC level. 58.9% and 79.8% of laboratories achieved an intra-laboratory CV &lt; 1.5% for low and high QC levels, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both inter-laboratory and intra-laboratory variations of HbA1c measurement have significantly decreased over the years. However, the difference among manufacturers still exists, and ongoing efforts are required to fully comply with clinical guideline requirements.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}