Journal of Clinical Laboratory Analysis最新文献

{"title":"Improving the Diagnosis and Follow-Up of Chronic Myeloid Leukemia Using Conventional and Molecular Techniques","authors":"Noor Al-Huda A. Bahar,&nbsp;Mushtak T. S. Al-Ouqaili,&nbsp;Nabeel M. Talib","doi":"10.1002/jcla.70001","DOIUrl":"10.1002/jcla.70001","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The Philadelphia chromosome (Ph) represented a finding of chronic myeloid leukemia (CML) in most cases which formed from t (9; 22) (q34; q11) resulting in the Breakpoint cluster region-Abelson tyrosine-protein kinase1 (BCR-ABL1) fusion gene. Assuming CCE's inaccuracies in diagnosing CML and FISH's limitations with low BCR-ABL1 percentages, a Predicted-FISH (Pred-FISH) was developed. This model predicts treatment response during follow-up by integrating qRT-PCR results, White Blood Cell (WBC) counts, and Cytogenetic Response data.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Quantitative Real-Time Polymerase Chain Reaction Analysis (qRT-PCR), fluorescence in situ hybridization (FISH), and Conventional Cytogenetic Examination (CCE or Karyotyping) have been used in the detection and follow-up of CML patients. The study included 110 individuals, divided into three groups: 31.82% (35 individuals) were newly diagnosed CML patients, another 22.73% (25 individuals) were healthy control samples, and the remaining 45.45% (50 individuals) were previously diagnosed CML patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Include BCR-ABL1 fusion gene levels detected by qRT-PCR, Ph chromosome presence t (9; 22) (q34; q11) observed by CCE, and WBC counts. The FISH test, used to confirm disease in new patients before treatment, was compared to CCE results due to its insensitivity in certain conditions. Data from CCE, FISH, qRT-PCR, and WBC for newly diagnosed patients provided a standard for evaluating the Predicted-FISH.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The FISH technique excels in disease detection with over 98% accuracy and high sensitivity. QRT-PCR is effective for monitoring CML and BCR-ABL1 gene levels, indicating MMR and DMR. CCE, while useful for posttreatment monitoring, is less accurate in measuring treatment response over time.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measuring Hemoglobin A1C with Frozen Packed Cell and Frozen Whole Blood Samples in an Epidemiologic Study: The Reasons for Geographic and Racial Differences in Stroke Study","authors":"Debora Kamin Mukaz,&nbsp;Stephanie Tison,&nbsp;D. Leann Long,&nbsp;April P. Carson,&nbsp;Kelly J. Hunt,&nbsp;Suzanne E. Judd,&nbsp;Mary Cushman","doi":"10.1002/jcla.25121","DOIUrl":"10.1002/jcla.25121","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Hemoglobin A1c (HbA1c) measurement in epidemiology studies could be increased if reliability of measurements in frozen stored samples was known. In the Reasons for Geographic And Racial Differences in Stroke, a longitudinal study of 30,239 Black and White U.S. adults, we investigated reliability of HbA1c measurements for two types of samples stored at −80°C for up to 14 years.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Among 917 participants without diabetes, HbA1c was measured in 2017 in frozen packed cells from the first visit (2003–2007) and in frozen whole blood samples from the second visit (2013–2016). To study reliability, associations between HbA1c and glycemia-related characteristics were examined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Each 10 mg/dL greater fasting glucose was associated with 0.08% (95% CI: 0.05%–0.11%) greater HbA1c in frozen packed cells (Visit 1) and 0.10% (95% CI: 0.08%–0.12%) greater HbA1c in whole blood (Visit 2). HbA1c was also similarly higher with both methods with increasing age, gender, systolic blood pressure, body mass index, high-density lipoprotein, triglycerides, C-reactive protein, and hemoglobin. Using both methods, ≤ 3.5% would be classified with diabetes based on HbA1c ≥ 6.5%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In REGARDS participants without diabetes, HbA1c measurement appeared reliable in frozen packed cells or whole blood under long-term storage, suggesting acceptability for study of the epidemiology of HbA1c.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Long Non-Coding RNA TGLC15 Advances Hepatocellular Carcinoma by Stabilizing Sox4","authors":"","doi":"10.1002/jcla.70002","DOIUrl":"10.1002/jcla.70002","url":null,"abstract":"<p><b>RETRACTION</b>: Y. Chen, F. Huang, L. Deng, Y. Tang, D. Li, T. Wang, Y. Fan, Q. Tao, and D. Tang, “Long Non-Coding RNA TGLC15 Advances Hepatocellular Carcinoma by Stabilizing Sox4,” <i>Journal of Clinical Laboratory Analysis</i> 34, no. 1 (2020): e23009, https://doi.org/10.1002/jcla.23009.</p><p>The above article, published online on 08 September 2019 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Rong Fu; and Wiley Periodicals LLC. The retraction has been agreed upon following an investigation into concerns raised by a third party, which revealed inappropriate duplication of image panels (Figure 2E) between this article and several other articles that were previously published in a different scientific context. Thus, the editors consider the conclusions of this manuscript substantially compromised.</p><p>D. Tang, listed as the corresponding author, asked to retract the article and stated that they were not involved in or given consent to this publication and the corresponding email address mentioned under their name does not belong to them.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of HIV Drug-Resistance Mutations and Antiretroviral Efficacy Among Vietnamese Patients After Failure of 5-Year First-Line Therapy","authors":"Than Manh Hung,&nbsp;Le Van Nguyen Bang,&nbsp;Le Van Duyet","doi":"10.1002/jcla.25157","DOIUrl":"10.1002/jcla.25157","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The emergence of drug-resistant mutations in human immunodeficiency virus (HIV) over time presents a challenge to treatment. We describe the development of drug-resistance mutations and ART efficacy reduction in Vietnamese patients with failure of first-line ART during a 5-year period.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This is a 5-year observational cohort study with HIV viral loads of patients evaluated annually for 5 years (2017–2022) at the hospitals in Vietnam. Patients with a viral load ≥ 1000 copies/mL were subjected to identifying mutations in reverse transcriptase, protease, and integrase to evaluate HIV resistance and the efficacy of ART.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After 5 years of monitoring the HIV load of 2932 patients on ART, 75 (2.56%) patients had concurrent virological failure at all 5 years. In 2017, only 2/75 HIV strains possessed Protease Inhibitor (PI) resistance mutations, while 75/75 HIV strains had both Nucleoside Reverse Transcriptase Inhibitors (NRTIs) and Non-Nucleoside Reverse Transcriptase Inhibitors (NNRTIs) resistance mutations. Only four PI resistance variants were found, while 40 and 32 mutations were resistant to NRTIs and NNRTIs. After 5 years, the number of HIV PI resistance mutations had increased to 14, with 13 new mutations emerging. There were six novel mutations associated with resistance to NRTIs, NNRTIs, and the proportion of preexisting mutations increased from 1.3% to 13.3%. Furthermore, HIV sensitivity to ART decreased from 2.7% to 18.6%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>After 5 years, HIV had increased resistance mutations to PIs, NRTIs, and NNRTIs, with PI resistance mutations increasing the most rapidly, and the decrease in HIV sensitivity to PIs was higher than that to NRTIs and NNRTIs.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Urinary Red Blood Cell Distribution (URD) and Dysmorphic Red Blood Cells for Detecting Glomerular Hematuria: A Multicenter Study","authors":"A-Jin Lee,&nbsp;Hae In Bang,&nbsp;Sun Min Lee,&nbsp;Dongil Won,&nbsp;Myung Seo Kang,&nbsp;Hyun-ji Choi,&nbsp;In Hee Lee,&nbsp;Chang-Ho Jeon","doi":"10.1002/jcla.25159","DOIUrl":"10.1002/jcla.25159","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The clinical utility of urinary red blood cell (RBC) distribution (URD) remains limited. This study aimed to compare the diagnostic performance of URD and dysmorphic RBC (dRBC) in a multicenter study.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study enrolled 703 patients who visited four tertiary medical centers in Korea. Patients were classified into glomerular diseases with biopsy (<i>N</i> = 169), renal diseases including chronic kidney disease (<i>N</i> = 194), nephrotic syndrome (NS; <i>N</i> = 88), tubulointerstitial diseases (<i>N</i> = 36), acute kidney injury (<i>N</i> = 32), others (<i>N</i> = 10), and extrarenal diseases (<i>N</i> = 174). Renal parameters, urine microscopic examination, urinalysis, and URD assessments were conducted. The diagnostic performances of dRBC and URD were evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Median values of both dRBC and URD were significantly elevated in patients with glomerular diseases. URD exhibited a significant correlation with dRBC (<i>r</i> = 0.536) and albumin creatinine ratio (<i>r</i> = 0.186), while no significant correlation was observed with specific gravity (<i>r</i> = −0.03). Among renal diseases, dRBC and URD values were notably higher in patients with NS. The agreement rate between dRBC and URD results was 78.3% (112/143), with 31 instances showing discrepancies. ROC curve analysis comparing glomerular and extrarenal diseases yielded cutoff values of 18% for dRBC and 31.9% for URD, resulting in corresponding areas under the curve (AUC) of 0.79 and 0.83, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>URD exhibited a comparable diagnostic performance, as indicated by a similar AUC value to that of dRBC, while offering the added advantage of providing objective and standardizable results. This attribute enhances its utility as a parameter for distinguishing between patients with glomerular hematuria (GH) and those with non-GH.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Synthetic Antimicrobial Peptides Based on Genomic Analysis of Streptococcus salivarius","authors":"Vishakha Grover,&nbsp;Ashish Jain,&nbsp;Amit Bhardwaj,&nbsp;Manjula Mehta,&nbsp;Suraj Arora,&nbsp;Youssef A. Algarni,&nbsp;Shashit Shetty Bavabeedu,&nbsp;Gotam Das,&nbsp;Ahmed Babiker Mohamed Ali,&nbsp;Naseer Ahmed,&nbsp;Artak Heboyan","doi":"10.1002/jcla.25156","DOIUrl":"10.1002/jcla.25156","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>In the oral environment, the production of bacteriocins or antimicrobial peptides (AMPs) plays a crucial role in maintaining ecological balance by impeding the proliferation of closely related microorganisms. This study aims to conduct in silico genome screening of <i>Streptococcus salivarius</i> to identify potential antimicrobial compounds existing as hypothetical peptides, with the goal of developing novel synthetic antimicrobial peptides.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Draft genomes of various oral <i>Streptococcus salivarius</i> strains were obtained from the NCBI database and subjected to analysis using bioinformatic tools, viz. Expert Protein-Analysis System (Expasy), UniProt Knowledgebase (UniProtKB), European Molecular Biology Open Software Suite (EMBOSS), Pepwheel, and PEP-FOLD Peptide Structure Prediction Server. The antimicrobial potential of peptides was assessed through the Antimicrobial Peptide Database (AMP) and Bactibase. Two short peptides, viz. synthetic antimicrobial peptides (SAMPs), were designed based on current knowledge of hydrophobic and cationic residues, synthesized, and their efficacy against biofilm formation was evaluated with standard microbiological methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The synthesized short peptides reduced the growth and effectively inhibited biofilm formation by specific oral microbial strains, demonstrating their potential as antimicrobial peptides. Furthermore, the alignment of bacteriocin biosynthetic clusters among streptococcus strains revealed variations in putative bacteriocin amino acid sequences across different strains of the same organism.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p><i>Streptococcus salivarius</i> emerges as a promising bioresource for the development of novel antimicrobial agents, particularly for combating biofilm-associated oral infections.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25156","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Chromosomal Mosaicism in Prenatal Diagnosis: The Complementary Roles of Chromosomal Microarray Analysis and Karyotyping","authors":"Chenxia Xu,&nbsp;Yi Xiong,&nbsp;Degang Wang,&nbsp;Sheng Zhang,&nbsp;Xuewei Wu,&nbsp;Miaoyuan Li","doi":"10.1002/jcla.25154","DOIUrl":"10.1002/jcla.25154","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To explore the impact of in vitro cell subculture on prenatal diagnostic sample results and compare the efficacy of conventional karyotyping and chromosomal microarray analysis (CMA) in detecting chromosome mosaicism.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We conducted a retrospective analysis of G-banding karyotyping and CMA data from 2007 amniocentesis cases to investigate chromosome mosaicism.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Chromosome mosaicism was detected in 1.49% of cases (30/2007). Sex chromosome mosaicism was the most common form of mosaicism. Among the 30 mosaicisms, 18 results were consistent between the two methods. In four cases, CMA indicated mosaicism but the karyotypes were normal. In eight cases, CMA was normal while the karyotypes suggested mosaicism.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>CMA and karyotyping complement each other in prenatal genetic diagnosis. Combining both methods enhances detection accuracy, particularly in cases of chromosomal mosaicism, which may be missed after the subculture of adherent cells in karyotype analysis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Plasmid-Mediated Fluoroquinolone Resistance Genes in Pseudomonas aeruginosa Among Patients at Aleppo University Hospital, Syria","authors":"Roula Shanan,&nbsp;Nihad Yousef,&nbsp;M. Emad Balid,&nbsp;Zaher S. Tahan","doi":"10.1002/jcla.25153","DOIUrl":"10.1002/jcla.25153","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p><i>Pseudomonas aeruginosa</i> is a significant opportunistic pathogen, especially in hospital-acquired infections, with plasmid-mediated fluoroquinolone resistance posing a major healthcare threat. This research aimed to isolate fluoroquinolone-resistant <i>P. aeruginosa</i> from patients at Aleppo University Hospital, assess the prevalence of fluoroquinolone resistance, confirm molecular identity, identify plasmid-associated resistance genes, and investigate virulence factors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 430 samples were collected from patients and cultured on selective media for identification. Molecular confirmation was achieved through PCR techniques. Various media were used to assess virulence factors and antibiotic resistance while also investigating the prevalence of resistance-related genes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The study identified 29 fluoroquinolone-resistant <i>P. aeruginosa</i> isolates. These strains exhibited complete resistance to penicillins and all four generations of cephalosporins while remaining 100% sensitive to colistin. Notably, both hemolysin and gelatinase production rates were found to be 100%, and 48.2% of the isolates formed strong biofilms. The <i>aac(6′)-Ib</i> gene was present in 72.4% of the isolates, the <i>qnrS</i> gene in 44.8%, and the <i>qnrB</i> gene in 13.7%. Additionally, 37.8% of the isolates contained two types of resistance genes, while 62% had one type. Importantly, all resistant isolates (100%) possessed at least four virulence factors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The findings indicate a prevalence of plasmid-associated fluoroquinolone resistance genes in the studied isolates. It is recommended to rationalize fluoroquinolone use to preserve their effectiveness against multidrug-resistant strains.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Discovery of New Antibody in Autoimmune Disease Using a Novel Approach of Coombs Test Based on Flow Cytometry Method","authors":"Anwar Ullah,&nbsp;Xuewei Ding,&nbsp;Xia Qi*,&nbsp;Hui Liu*","doi":"10.1002/jcla.25148","DOIUrl":"10.1002/jcla.25148","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objective</h3>\u0000 \u0000 <p>The objective of this study was to investigate the antibody to RBC in autoimmune disease patients with ANA using sensitive Coombs test based on flow cytometry method.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Antinuclear antibodies (ANA) of autoimmune disease patients were added to red blood cells (RBCs) of blood group O. At the same time, healthy individuals' serums were also checked. The sample tubes were incubated for 30 min at 37°C. After incubation, each sample was analyzed on flow cytometry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The agglutination of antinuclear antibodies in autoimmune patients was observed, while in healthy people, there was no agglutination between RBCs and serum. A significant difference was found between the disease group and healthy group (<i>p</i> &lt; 0.0001) showing that a statistical analysis was conducted to compare the presence of ANA agglutination between the two groups. The reported p-value of less than 0.0001 shows that the observed difference is highly significant. The serum stability test conducted over ten consecutive days demonstrated a CV of 6.90% in the test results, indicating favorable stability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In conclusion, this study emphasizes the effectiveness of flow cytometry as a valuable tool for detecting RBC-bound antibodies and new antibodies in autoimmune disease patients. Its high sensitivity and accuracy have the potential to greatly improve diagnostic capabilities in clinical laboratories.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 3","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B4GALNT1 Regulates Hepatocellular Carcinoma Cell Proliferation and Apoptosis via the PI3K–AKT–mTOR Pathway","authors":"Lihan Bie,&nbsp;Guangquan Chen,&nbsp;Xin Lei,&nbsp;Feng Xiao,&nbsp;Zheng Xu,&nbsp;Zhouhong Xiang,&nbsp;Zhicheng Lu,&nbsp;Xiudi Jiang","doi":"10.1002/jcla.25155","DOIUrl":"10.1002/jcla.25155","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Hepatocellular carcinoma (HCC) is a ubiquitous malignancy linked to significant mortality. The abnormal expression of β-1,4-N-acetyl-galactosaminyltransferase 1 (B4GALNT1) seemed to be implicated in tumorigenesis. Nonetheless, this enzyme's roles in HCC are unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>By analyzing the TCGA_LIHC, GSE77509, and GSE135631 datasets, the levels of <i>B4GALNT1</i> expression in HCC and surrounding non-cancerous tissues were compared. The prognostic implications of <i>B4GALNT1</i> were assessed using the Cox regression analysis (CRA). The relationship of <i>B4GALNT1</i> mutations with CpG island methylation levels and prognosis was examined by analyzing the cBioPortal and MethSurv databases. We sifted the evidence of <i>B4GALNT1</i> expression correlating with 28 immune cell types' infiltration by harnessing the “GSVA” R package. To delve into the influences of genes associated with B4GALNT1 on HCC, we implemented gene set enrichment analysis (GSEA). We constructed a lentiviral vector expressing B4GALNT1 and knocked down <i>B4GALNT1</i> in HepG2 cells. The resulting effects on HCC cell proliferation and apoptosis were analyzed via cell proliferation assays and flow cytometry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>HCC tissues presented significant <i>B4GALNT1</i> overexpression relative to surrounding non-cancerous tissues, marking it as a standalone risk factor for HCC progression. Methylation levels of two CpG islands were high, suggesting poor prognosis. It was detectable that <i>B4GALNT1</i> expression interrelated with the infiltration extent of natural killer T cells in HCC tissues. B4GALNT1-fueled cell proliferation and enhanced resistance to apoptosis in HCC cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>B4GALNT1 is a strong regulator of HCC progression and holds promise as a marker for prognosis and a hallmark for therapy in HCC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}