Expression Profile of Serum CircFUNDC1 and CircUHRF1 Can Differentiate Between Colorectal Cancer and Inflammatory Bowel Diseases (Ulcerative Colitis and Crohn's Disease).

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-16 DOI:10.1002/jcla.70039

Marwa A Ali, Olfat G Shaker, Eman M Ezzat, Abeer A Khalefa, Naglaa A Ahmed, Raghda Yahia Abu El-Ela, Amal A Ibrahim Amin, Fatma Abdel Hamid Mohamed, Marwa Mamdouh Ahmed Abdelhafeez, Mohamed Saad Zaghloul Ahmed, Marwa N AbdelHafez, Abdulrhman Khaled Al Abdulqader, Reham Fares

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引用次数: 0

Abstract

Background: Colorectal cancer (CRC) is a worldwide burden. Circular RNAs are promising biomarkers for diagnosing and prognosis of CRC.

Objective: To investigate the possible association of sera levels of CircFUNDC1 and CircUHRF1 expression with predisposition and clinicopathological findings in CRC, ulcerative colitis (UC), and Crohn's disease (CD) in Egyptian patients.

Methods: The serum levels of CircFUNDC1 and CircUHRF1 were evaluated in 113 Egyptian subjects divided into four groups; CRC (31), UC (26), and CD (25) and compared to healthy controls (31) using quantitative polymerase chain reaction.

Results: The median values of log2 serum fold change (FC) of CircFUNDC1 in CRC, UC, and CD patients were 9.11, 6.58, and 6.17, respectively. It was upregulated in all case groups. CRC, UC, and CD patients had significantly higher serum CircFUNDC1 levels than controls (p < 0.001). However, there were no significant differences among patient groups (CRC, UC, and CD). The medians of log 2 of serum FC CircUHRF1 in patients with CRC, UC, and CD were -2.00, 3.33, and 3.12, respectively. The CircUHRF1 serum level was lower in the CRC group of patients, with no significant difference between the CRC group and the controls. Serum CircUHRF1 was significantly overexpressed in patients with UC and CD compared to the CRC groups or controls (p < 0.001). By Roc curve analysis, both genes can differentiate CRC patients from inflammatory bowel disease (IBD) patients or healthy controls with p < 0.05.

Conclusion: Serum CircFUNDC1 is a biomarker for CRC, while CircUHRF1 is a biomarker of IBD.

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血清CircFUNDC1和cirhrf1的表达谱可以区分结直肠癌和炎症性肠病（溃疡性结肠炎和克罗恩病）

背景：结直肠癌（CRC）是一个世界性的负担。环状rna是CRC诊断和预后的重要生物标志物。目的：探讨CircFUNDC1和cirhrf1的血清表达水平与埃及患者CRC、溃疡性结肠炎（UC）和克罗恩病（CD）的易感性和临床病理表现之间的可能关联。方法：将113例埃及人分为4组，测定血清CircFUNDC1和cirhrf1水平；CRC (31)， UC（26）和CD(25)，并使用定量聚合酶链反应与健康对照（31）进行比较。结果：CircFUNDC1在CRC、UC和CD患者的log2血清折叠变化（FC）中位数分别为9.11、6.58和6.17。它在所有病例组中都是上调的。CRC、UC和CD患者血清CircFUNDC1水平明显高于对照组(p)。结论：血清CircFUNDC1是CRC的生物标志物，而circhrf1是IBD的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.