Journal of Clinical Laboratory Analysis最新文献

{"title":"Drug Resistance and Epidemiological Analysis of Carbapenem-Resistant Enterobacteriaceae in a Brain and Psychiatric Hospital of South China.","authors":"Lei Yue, Xiumei Zhu, Yuyan Kuang, Yulong Lin, Haiying Liu","doi":"10.1002/jcla.70073","DOIUrl":"https://doi.org/10.1002/jcla.70073","url":null,"abstract":"<p><strong>Background: </strong>Carbapenem-resistant Enterobacteriaceae (CRE) has increasingly become a global health problem, and the drug resistance and epidemiology of its infection are important for disease prevention and control.</p><p><strong>Methods: </strong>The CRE isolates detected in the hospital from July 2021 to April 2023 were collected, then the antibiotic sensitivity was detected by an automatic microbial analyzer. Polymerase chain reaction (PCR) was performed to detect carbapenem-resistance genes. The homology and epidemiological characteristics of the strains of Enterobacteriaceae were analyzed by repeated common sequence PCR(ERIC-PCR).</p><p><strong>Results: </strong>A total of 94 non-repeating CRE isolates were collected mainly from the respiratory tract (67.02%) and urinary tract (22.34%). Among these, 73 strains (77.66%) were carbapenem-resistant Klebsiella pneumoniae (CRKP), while 21 strains (22.34%) were carbapenem-resistant Escherichia coli (CREC). Antibiotic susceptibility testing revealed a low resistance rate to tigecycline (2.13%). Analysis of CRE resistance genes demonstrated that blaKPC accounted for 93.15% of CRKP isolates (68/73), while blaNDM was present in 90.48% of CREC isolates (19/21). In the homology analysis, CRKP isolates were divided into five clusters with C_KP type representing the majority at 80.82% prevalence (59/73). Similarly, CREC could be classified into four clusters with A_EC type being predominant at 80.95% among the isolated strains (17/21). The detection of CRE strains involved multiple departments in two separate wards of this hospital, located approximately 28 km from each other.</p><p><strong>Conclusions: </strong>CRE infection in this hospital has high homology and wide distribution; it is necessary to strengthen prevention and control measures to control CRE nosocomial infection spread.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70073"},"PeriodicalIF":2.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistent AST Elevation in a Patient With Ovarian Cancer: A Rare Diagnostic Challenge.","authors":"Lechuang Chen, Yu Zhang, Qing H Meng","doi":"10.1002/jcla.70072","DOIUrl":"https://doi.org/10.1002/jcla.70072","url":null,"abstract":"<p><strong>Background: </strong>Persistent elevation of aspartate aminotransferase (AST) is commonly indicative of liver injury or disease, but isolated AST elevation without concurrent alanine aminotransferase (ALT) increase is rare and difficult to diagnose. While AST is non-specific and found in various tissues, its isolated elevation is due to less common conditions, such as macro-AST, where AST binds with immunoglobulins creating a high-molecular-weight complex that affects serum activity.</p><p><strong>Case description: </strong>A 68-year-old female with a history of high-grade serous ovarian cancer (HGSOC) who had persistent isolated AST elevation for several years. Evaluations including physical exams, imaging, and routine liver function tests showed no evidence of hepatic or muscular disease. The polyethylene glycol (PEG) precipitation significantly reduced serum AST activity, confirming the presence of the macro-enzyme form of AST (macro-AST).</p><p><strong>Conclusion: </strong>This case highlights the rare and novel occurrence of macro-AST in a patient with ovarian cancer. It emphasizes the importance of considering macro-AST in the differential diagnosis of isolated AST elevation, particularly in patients without clear evidence of liver or muscular disease. Recognizing this benign condition can prevent unnecessary diagnostic procedures and anxiety.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70072"},"PeriodicalIF":2.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Interleukin-23 and Interleukin-17 in Peri-Implant Crevicular Fluid of Subjects With Peri-Implant Disease: A Systematic Review.","authors":"Mario Alberto Alarcón-Sánchez, Julieta Sarai Becerra-Ruiz, Ruth Rodríguez-Montaño, Sarah Monserrat Lomelí-Martínez, Lilibeth-Stephania Escoto-Vasquez, Artak Heboyan","doi":"10.1002/jcla.70071","DOIUrl":"10.1002/jcla.70071","url":null,"abstract":"<p><strong>Background: </strong>Activation of the IL-23/IL-17 cytokine axis could trigger peri-implant bone loss. The aim of this review was to analyze whether in people with peri-implantitis (PI) and peri-implant mucositis (PM) the concentrations of the interleukin-23 and interleukin-17 in peri-implant crevicular fluid (PICF) are elevated compared to people with healthy dental implants (HDI).</p><p><strong>Methodology: </strong>The protocol of this study was registered in OSF (ID: 10.17605/OSF.IO/U8NBQ) and followed PRISMA guidelines. PECO criteria were used to formulate the research question. A search strategy was performed using PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar until November 15, 2024. A rigorous evaluation was performed, and the JBI tool was used to assess the quality of the cross-sectional and case-control studies.</p><p><strong>Results: </strong>Fourteen observational studies were included in this study, with a total of 587 participants carrying 601 dental implants. The control group was represented by 252 healthy implants, while the exposure group was represented by 113 implants with PM and 236 implants with peri-implantitis. The age range of the subjects varied from 40.8 to 68.6 years, with a mean age ± standard deviation of 53.9 ± 9.9 years. The concentration of the IL-23/IL-17 cytokine axis and isoforms (IL-17E and IL-17F) was higher in subjects with peri-implant disease compared to the healthy population. Most of the studies (92.8%) showed moderate quality.</p><p><strong>Conclusions: </strong>The concentrations of cytokines IL-23, IL-17, and IL-17E in PICF were higher in PI-affected dental implants, followed by PM-affected dental implants compared to HDI.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70071"},"PeriodicalIF":2.6,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144368897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal Diagnosis, Ultrasound Findings, and Follow-Up Evaluation of 16p13.11 Deletion and Duplication Syndromes: Preliminary Assessment of Fetal Genotype–Phenotype","authors":"Xiaojin Luo,&nbsp;Liping Wu,&nbsp;Jinshuang Song,&nbsp;Jinmao Xu,&nbsp;Ruchun Huang,&nbsp;Hongyan Niu,&nbsp;Fei Zhou,&nbsp;Yuanyuan Pei,&nbsp;Weiqiang Liu,&nbsp;Fengxiang Wei","doi":"10.1002/jcla.70051","DOIUrl":"10.1002/jcla.70051","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To analyze the ultrasound findings, single nucleotide polymorphism microarray (SNP array) results, pregnancy outcomes, and follow-up information of fetuses with 16p13.11 deletion or duplication.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This retrospective study collected data from 14 fetuses diagnosed with 16p13.11 deletion and 12 fetuses with 16p13.11 duplication. The study involved a review and analysis of maternal demographics, ultrasound findings, SNP-array results, pregnancy outcomes, and follow-up information.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The copy number variations (CNVs) observed ranged in size from 0.92 to 2.85 Mb for 16p13.11 deletions and from 0.89 to 2.84 Mb for duplications. These CNVs included seven OMIM genes: <i>NDE1</i>, <i>MYH11</i>, <i>ABCC1</i>, <i>XYLT1</i>, <i>MARF1</i>, <i>CEP20</i>, and <i>ABCC6</i>. Among the 14 fetuses with 16p13.11 deletions, seven (50.0%, 7/14) revealed abnormalities in ultrasound findings. Cardiovascular anomalies were present in five cases (35.7%, 5/14); two cases (14.3%, 2/14) showed lateral ventricular widening. Cases 2 and 14 were particularly noteworthy, as both presented complex malformations affecting multiple organs. Among the 12 fetuses with duplications, five cases (41.7%, 5/12) exhibited ultrasound abnormalities. Of these, three cases (25.0%, 3/12) presented with cardiovascular abnormalities; two cases (16.7%, 2/12) displayed widened lateral ventricles. Case 25 was particularly distinct, featuring complex multiorgan malformations that included widened lateral ventricles, tricuspid regurgitation, and a right ear malformation. Of the eight fetuses with 16p13.11 deletions whose pregnancies were continued, three exhibited neurodevelopmental abnormalities. Ten fetuses with 16p13.11 duplications that were followed up, two cases showed neurodevelopmental abnormalities.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our study expanded the clinical phenotype spectrum of fetuses with 16p13.11 deletion and duplication and conducted a preliminary evaluation of prenatal ultrasound findings in conjunction with postnatal clinical phenotypes. The primary manifestations observed in fetuses with 16p13.11 deletion and duplication are likely to be cardiovascular malformations and widened lateral ventricles.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 13","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Univariate and Multivariate Reference Interval Methods.","authors":"Esra Kutsal Mergen, Sevilay Karahan","doi":"10.1002/jcla.70070","DOIUrl":"https://doi.org/10.1002/jcla.70070","url":null,"abstract":"<p><strong>Background: </strong>In clinical practice, reference intervals play a pivotal role in interpreting laboratory test results. Yet, when several tests are taken into consideration simultaneously, the traditional univariate intervals might not suffice due to the elevated risk of Type 1 errors.</p><p><strong>Methods: </strong>This study introduces and evaluates two multivariate reference interval techniques: one based on Mahalanobis distance and the other an adaptation of the multivariate confidence interval (MCI). Using Monte Carlo simulations, we focused our assessments on the interplay between \"serum ferritin and transferrin saturation\" values.</p><p><strong>Results: </strong>Upon evaluation, it became evident that the multivariate methods significantly reduced false positives. They presented enhanced accuracy over traditional univariate intervals. Notably, the method involving Mahalanobis distance stood out in terms of efficacy.</p><p><strong>Contributions: </strong>Beyond presenting novel techniques, our research underscores the importance and potential of using multivariate approaches in clinical lab settings. The findings can guide better medical decision making, ensuring optimized allocation of healthcare resources.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70070"},"PeriodicalIF":2.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Nerve Growth Factor Is Associated With Early Postoperative Shoulder Stiffness in Patients With Degenerative Rotator Cuff Tears","authors":"ZengXin Li,&nbsp;ZengYan Wang,&nbsp;Bo Sun,&nbsp;DengFeng Wang,&nbsp;JianPing Guo,&nbsp;Hua Pan","doi":"10.1002/jcla.70043","DOIUrl":"10.1002/jcla.70043","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study was to investigate the correlation between plasma nerve growth factor (NGF) and postoperative range of motion (ROM) in patients with rotator cuff tears (RCT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>From January 2022 to March 2024, a total of 135 patients with degenerative RCT were included. The patients were divided into Stiff group (<i>n</i> = 42) and Non-Stiff group (<i>n</i> = 93) accordingly. The receiver operating characteristic curve (ROC) and area under the curve (AUC) were drawn to evaluate the predictive value of plasma NGF for postoperative joint stiffness. The Spearman test was used to analyze the relationship between plasma NGF and joint motion, VAS score, and joint function scores (UCLA score and Constant score). The relationship between plasma NGF and postoperative ROM and risk of joint stiffness was evaluated by restricted cubic spline (RCS) analysis of independent variables.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In the preoperative and postoperative comparison, the two groups improved in joint flexion, VAS pain score, and joint function, except that the external rotation of the Stiff group did not change before and after surgery. After surgery, the ROM of the Stiff group was still significantly limited, and the ROM and joint function scores were lower than those of the Non-Stiff group (<i>p</i> &lt; 0.01).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study suggests that reduced plasma NGF in patients with degenerative RCT may be associated with early shoulder stiffness, showing a negative non-linear dose–response relationship. On a clinical basis, plasma NGF is beneficial to quantify the risk of early shoulder stiffness after RCT.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 13","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of HIV-1 Antibodies in Saliva of Persons Living With HIV Using Blood-Based First Response HIV 1-2.O Card Test.","authors":"Enoch Aninagyei, Comfort Addo Boatey, Gifty Larbi, Wilson Bright Tsidi, Raphael Eyram Amemo, Ebenezer Tawiah Nyarkotey, Godknows Afenya, Desmond Omane Acheampong","doi":"10.1002/jcla.70069","DOIUrl":"https://doi.org/10.1002/jcla.70069","url":null,"abstract":"<p><strong>Background: </strong>This study tested HIV-1 antibodies in saliva samples (sHIV-1 Ab) collected by the Self-Lollisponge device.</p><p><strong>Methods: </strong>Blood and saliva from confirmed persons with HIV and HIV-negative controls were analyzed for HIV-1/2 antibodies using the blood-based First Response HIV 1-2.O Card Test. The sampling device containing sHIV-1 Ab was stored at 6°C for 60 days, with intermittent testing on days 2, 5, 10, 20, 30, and 60. Regression analysis was done to assess the relationship between the presence of sHIV-1 Ab and independent variables.</p><p><strong>Results: </strong>The sensitivity and the specificity of detecting sHIV-1 Ab were 72.9% (95% CI: 63.92%-80.65%) and 100% (95% CI: 92.89%-100.00%), respectively. The presence of opportunistic infections (AOR = 13.1, p < 0.001), having stomatorrhagia (AOR = 4.56, p = 0.0022), and hyperviremia (> 201 copies/mL) (AOR = 4.91, p = 0.0225) heightened sHIV-1 Ab detection. Furthermore, fatigue (AOR = 12.1, p = 0.0024), fever (AOR = 3.5, p = 0.0144), and weight loss (AOR = 10.9, p = 0.0318) increased the odds of having sHIV-1 Ab in persons living with HIV (PLWHIV). sHIV-1 Ab was identified in over 90% of PLWHIV with opportunistic infections (OIs) and stomatorrhagia, OIs and hyperviremia, and stomatorrhagia and hyperviremia. Upon storage for 60 days, the sHIV-1 Ab was detected in all the samples.</p><p><strong>Conclusion: </strong>Saliva could be an alternative to blood for diagnosing HIV. In addition, the Self-Lollisponge device was found to be user-friendly, acquiescent to all settings, and cheap, and can preserve sHIV-1 Ab for at least 60 days.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70069"},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age Estimation From Blood Test Results Using a Random Forest Model.","authors":"Satomi Kodera, Osamu Yokoi, Masaki Kaneko, Yuka Sato, Susumu Ito, Katsuhiko Hata","doi":"10.1002/jcla.70064","DOIUrl":"https://doi.org/10.1002/jcla.70064","url":null,"abstract":"<p><strong>Background and objectives: </strong>From a preventive medicine perspective, this study aims to clarify the role of screening data in aging and health problems by estimating age from screening data and verifying the number of data items required in widely used screening tests.</p><p><strong>Materials and methods: </strong>A random forest model was applied to 11554 men and women (3043 and 8511, respectively) aged 0-95 years who underwent screening tests (60 blood tests, 8 urine tests and 2 saliva tests) between February 2020 and August 2023. All analyses were conducted in Python 3.10.12.</p><p><strong>Results: </strong>Using all 71 items including gender, a high accuracy of R² = 0.7010 was achieved with 9243 training datasets (80% of total). R² decreased slightly to 0.6937 when data items were reduced to 15 by removing less important variables. When datasets numbered fewer than 800 or data items fewer than 7, R² fell below 0.6. Notably, postmenopausal women tended to have higher estimated ages compared to premenopausal women.</p><p><strong>Conclusions: </strong>Age estimation from blood data using the random forest model (blood age) is sufficiently precise for assessing physical aging state. Blood age, as well as other biological ages estimated from various omics estimators, was shown to be a very promising method for exploring the problems of aging such as metabolic syndrome and frail syndrome.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70064"},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial tRNAGlu 14687A>G May Be A Novel Mutation for Type 2 Diabetes Mellitus","authors":"Xiaojuan Rao,&nbsp;Liran Xie,&nbsp;Shuangwei Shi,&nbsp;Yifan Fang","doi":"10.1002/jcla.70056","DOIUrl":"10.1002/jcla.70056","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Sequence alternations in mitochondrial genomes, especially in mitochondrial tRNA (mt-tRNA), are closely related to type 2 diabetes mellitus (T2DM); however, the detailed molecular mechanism is still largely undetermined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Herein, we reported a T2DM Chinese family by using molecular and biochemical analyses. The mtDNA mutations in this pedigree were detected by PCR and Sanger sequencing. Moreover, phylogenetic analysis was used to assess the pathogenic mitochondrial DNA (mtDNA) mutation. We further evaluated mt-tRNA stability levels and mitochondrial functions in cybrids with and without the m.14687A&gt;G mutation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Members of this family expressed variable clinical phenotypes. Screening for the entire mitochondrial genomes revealed the occurrence of a novel m.14687A&gt;G mutation, which was located at position 60 in the TψC loop of tRNA^Glu, and that position was important for tRNA structure and function. By establishing cybrids derived from three diabetic patients carrying the m.14687A&gt;G mutation and three healthy individuals without this mutation, we noticed that this mutation caused approximately 52% reduction in tRNA^Glu stability level (<i>p</i> &lt; 0.0001). The 14687G cybrid showed more severely impaired mitochondrial functions than the 14687A cybrid: mtDNA content, ATP, and mitochondrial membrane potential (MMP) and OXPHOS enzyme activities were markedly decreased. But the levels of reactive oxygen species (ROS) were significantly increased.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our finding revealed that the novel m.14687A&gt;G mutation resulted in aberrant mt-tRNA metabolism and mitochondrial dysfunctions, which should be regarded as a pathogenic mutation for T2DM.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 13","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning Reveals the Value of Unconventional T Lymphocytes in Sepsis and Prognosis of Elderly Patients With Severe Lower Respiratory Tract Infections.","authors":"Tianqi Qi, Qian Gao, Yan Song, Yulin Li, Yanlan Yao, Xinyue Liu, Manyu Li, Jingxian Yang, Qi Hao","doi":"10.1002/jcla.70065","DOIUrl":"https://doi.org/10.1002/jcla.70065","url":null,"abstract":"<p><strong>Objective: </strong>This study enrolled 119 elderly patients with severe lower respiratory tract infections (LRTIs) and used machine learning (ML) to evaluate the predictive value of unconventional T lymphocytes (uT cells) in sepsis and 90-day prognosis.</p><p><strong>Methods: </strong>We used random forest (RF) and LASSO analyses to screen model uT cells (identified by RF-LASSO overlapping). The ML models, including LR, LDA, RandomForest, XGBoost, KNN, QDA, NaiveBayes, and ANN, were developed. These models were evaluated and compared based on accuracy, precision, recall, F1 score, sensitivity, specificity, area under the ROC curve (AUROC), and Brier score.</p><p><strong>Results: </strong>Two T cells were identified as factors of sepsis diagnosis: CD3⁺ and CD4⁺CD25⁺CD127dim. The LDA model demonstrated superior performance, achieving an accuracy of 0.806, AUROC of 0.771, F1 score of 0.720, and a low Brier score of 0.182. Four T cells were identified for predicting the 90-day prognosis: CD3⁺, CD3⁺CD4⁺, CD4⁺CD28⁺, and CD4⁺CD25⁺CD127dim. For the 90-day prognosis, the LDA model again performed best, with an accuracy of 0.972, F1 score of 0.952, AUROC of 0.935, and a low Brier score of 0.059.</p><p><strong>Conclusions: </strong>The LDA model is optimal for both diagnosing sepsis and predicting the 90-day prognosis in elderly patients with severe LRTIs. Key T-cell markers identified for sepsis include CD3⁺ and CD4⁺CD25⁺CD127dim, while the 90-day prognosis model includes CD3⁺, CD3⁺CD4⁺, CD4⁺CD28⁺, and CD4⁺CD25⁺CD127dim T cells. These markers should be prioritized for clinical testing.</p><p><strong>Trial registration: </strong>Not applicable.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70065"},"PeriodicalIF":2.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}