Journal of Clinical Laboratory Analysis最新文献

Establishing Decisional Cutoff Values of Neurofilament Light Chains in Cerebrospinal Fluid Measured by Fully Automated Chemiluminescent Enzyme Immunoassay.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-01-15 DOI: 10.1002/jcla.25152

Luisa Agnello, Caterina Maria Gambino, Fabio Del Ben, Anna Maria Ciaccio, Concetta Scazzone, Marcello Ciaccio

{"title":"Establishing Decisional Cutoff Values of Neurofilament Light Chains in Cerebrospinal Fluid Measured by Fully Automated Chemiluminescent Enzyme Immunoassay.","authors":"Luisa Agnello, Caterina Maria Gambino, Fabio Del Ben, Anna Maria Ciaccio, Concetta Scazzone, Marcello Ciaccio","doi":"10.1002/jcla.25152","DOIUrl":"https://doi.org/10.1002/jcla.25152","url":null,"abstract":"Introduction: Neurofilament light chain (NfL) is one of the most important biomarkers in the field of clinical neurochemistry. Several analytical methods have been developed in the last decade. Recently, Fujirebio introduced a ready-to-use assay kit for measuring NfL levels in the cerebrospinal fluid (CSF) on the fully automated LUMIPULSE G System. In this study, we established the decisional cutoffs for CSF NfL.Materials and methods: We performed a retrospective observational study including patients with cognitive decline. CSF NfL levels were measured by two analytical methods: the NF-light ELISA kit (UmanDiagnostics) and the Lumipulse G1200 fully automated system (Fujirebio). We calculated the cutoffs for the Lumipulse, starting from the consolidated cutoffs of the ELISA method for each age and using the equation obtained by the regression analysis.Results: The study population consisted of 100 patients with cognitive decline. The median levels of CSF NfL measured by Lumipulse and ELISA were 776.5 ± 772.6 pg/mL and 473.5 ± 443.5 pg/mL, respectively, significantly different (p < 0.001). The Spearman's rank correlation coefficient was 0.962, indicating a robust positive correlation between the two measurement methods. The equation derived from the Passing-Bablok regression analysis was CSF CLEIA = -61.16 + 1.83 × CSF ELISA. Based on this equation, we defined the decisional cutoff values.Conclusions: Decisional cutoffs are fundamental tools for guiding clinicians to use biomarkers' results and interpretation appropriately. This is the first study establishing the decisional cutoff value of NfL measured by Lumipulse, a fully automated platform widely used in clinical laboratories.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25152"},"PeriodicalIF":2.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Correlation Between Hematological Parameters and Palpitation in Outpatients With COVID-19: A Retrospective Study.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-01-14 DOI: 10.1002/jcla.25137

Lei Zhang, Ting Chen, Ling Wei, Juan Ren, Jing Gong, Hui Yuan, QingJun Liu

{"title":"Potential Correlation Between Hematological Parameters and Palpitation in Outpatients With COVID-19: A Retrospective Study.","authors":"Lei Zhang, Ting Chen, Ling Wei, Juan Ren, Jing Gong, Hui Yuan, QingJun Liu","doi":"10.1002/jcla.25137","DOIUrl":"https://doi.org/10.1002/jcla.25137","url":null,"abstract":"Background: Research on heart injury caused by COVID-19 is limited to large observational and retrospective cohort studies using imaging or pathological data. Reported changes in the levels of myocardial markers in severe diseases have been limited, with few studies on mild infections. The effects of COVID-19 on cardiac function and changes in myocardial marker levels have not yet been reported.Methods: We analyzed data from outpatient blood samples collected at Beijing Anzhen Hospital during the 2022 COVID-19 outbreak and used the same periods in 2020 and 2021 as controls, focusing on changes in routine blood tests, coagulation, myocardial markers, and other blood indices in patients with palpitations.Results: The number of patients with palpitations increased by 4.87-fold during the COVID-19 mass outbreak in 2022. The indices of myocardial damage did not show any symptom-related increases but decreased within the normal range. The proportion of patients with palpitations whose D-dimer and fibrinogen/fibrin degradation product (FDP) values exceeded the reference ranges increased, as did the numbers of neutrophils, monocytes, and platelets. In this retrospective analysis, we found little change in the myocardial markers in patients with mild COVID-19.Conclusions: In patients with mild COVID-19, attention should be diverted from detecting myocardial markers to changes in coagulation test results, focusing on the levels of coagulation indices to improve circulation and prevent thrombosis.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25137"},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Clinical Value of Novel Inflammatory Biomarkers for Predicting Mycoplasma pneumoniae Infection in Children.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-01-12 DOI: 10.1002/jcla.25150

Liqun Shao, Bohai Yu, Ying Lyu, Shizhen Fan, Caizhen Gu, Hetong Wang

{"title":"The Clinical Value of Novel Inflammatory Biomarkers for Predicting Mycoplasma pneumoniae Infection in Children.","authors":"Liqun Shao, Bohai Yu, Ying Lyu, Shizhen Fan, Caizhen Gu, Hetong Wang","doi":"10.1002/jcla.25150","DOIUrl":"https://doi.org/10.1002/jcla.25150","url":null,"abstract":"Background: Mycoplasma pneumoniae (MP) is a major cause of community-acquired pneumonia (CAP), posing diagnostic challenges. This study evaluates novel inflammatory biomarkers, including neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR), systemic immune-inflammation index (SII) and system inflammation response index (SIRI) for MP diagnosis in children.Methods: Complete blood count (CBC) results of 424 children with MP infection and 150 health children were collected. NLR, MLR, PLR, SII and SIRI, were respectively calculated. Shapiro-Wilk test, Student's t-test, Mann-Whitney U-test and Pearson chi-squared test were used to analyze the clinical data of the patients and participants. Multiple logistic regression analysis was conducted based on the results of single factor analysis. Receiver operating characteristic (ROC) curve was drawn to evaluate the potential of the above biomarkers for MP infection.Results: Compared with the control group, white blood cell (WBC) count, neutrophil (NEU) count, monocyte (MON) count, NLR, MLR, PLR, SII and SIRI were significantly higher and lymphocyte count (LYM) and platelet (PLT) were significantly lower than those in MP group. The results of multivariate logistic regression analysis indicate that MLR and SIRI can serve as major risk factors for MP infection in children. The predictive accuracy of logistic regression model based on MLR and SIRI is 83.28%. The area under the curve (AUC) results showed that SIRI has better predicting value of MP infection (AUC = 0.892, Sensitivity = 75.7%, Specificity = 92.0%).Conclusion: This study described the significance of novel inflammatory biomarkers in children with MP infection and may provide new auxiliary diagnostic indicators for MP infection.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25150"},"PeriodicalIF":2.6,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleocapsid Antibodies as an Optimal Serological Marker of SARS-CoV-2 Infection: A Longitudinal Study at the Thomayer University Hospital.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-01-06 DOI: 10.1002/jcla.25149

Markéta Ibrahimová, Vladislava Jamriková, Kateřina Pavelková, Klára Bořecká

{"title":"Nucleocapsid Antibodies as an Optimal Serological Marker of SARS-CoV-2 Infection: A Longitudinal Study at the Thomayer University Hospital.","authors":"Markéta Ibrahimová, Vladislava Jamriková, Kateřina Pavelková, Klára Bořecká","doi":"10.1002/jcla.25149","DOIUrl":"https://doi.org/10.1002/jcla.25149","url":null,"abstract":"Background: The longitudinal study was conducted over the initial 2 years of the COVID-19 pandemic, spanning from June 2020 to December 2022, in healthcare workers (HCWs) of the Thomayer University Hospital. A total of 3892 blood samples were collected and analyzed for total nucleocapsid (N) antibodies. The aim of the study was to evaluate the dynamics of N antibodies, their relationship to the PCR test, spike (S) antibodies, interferon-gamma, and prediction of reinfection with SARS-CoV-2.Methods: Blood collections were performed in three rounds, along with questionnaires addressing clinical symptoms of past infection, PCR testing, and vaccination. Antibody measurements included total N antibodies (Roche Diagnostics) and postvaccination S antibodies (Euroimmun). Cellular immunity was tested by interferon-gamma release assay (Euroimmun).Results: At the end of the study, 35.9% of HCWs were positive for N antibodies, and 39.5% of HCWs had either known PCR positivity or N antibodies or both. Ten percent of participants had no knowledge of a COVID-19 infection and 35% of positive individuals exhibited no symptoms. The values of positive antibodies decrease over a period of 6 months to 1 year, depending on the initial value, and their dynamics are highly variable. The study also demonstrated that the highest levels of spike antibodies and interferon-gamma occur during so-called hybrid immunity.Conclusion: Nucleocapsid antibodies proved valuable in monitoring SARS-CoV-2 infection dynamics, and they may detect cases of SARS-CoV-2 infection missed by PCR tests. The study identified distinct patterns in antibody dynamics and protection of hybrid immunity during reinfection.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25149"},"PeriodicalIF":2.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significant Association Between Genetic Polymorphism of Insulin-Like Growth Factor-2 mRNA Binding Protein-2 and Type 2 Diabetes Mellitus: A Population-Based Case-Control Study.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-01-03 DOI: 10.1002/jcla.25147

Elmutuz H Elssaig, Eltayib H Ahmed-Abakur, Tarig M S Alnour, Mohammed A Alsubai, Abdalla Eltoum Ali, Mohammad Fahad Ullah, Nizar H Saeedi, Fahad Daefalah Alenzi

{"title":"Significant Association Between Genetic Polymorphism of Insulin-Like Growth Factor-2 mRNA Binding Protein-2 and Type 2 Diabetes Mellitus: A Population-Based Case-Control Study.","authors":"Elmutuz H Elssaig, Eltayib H Ahmed-Abakur, Tarig M S Alnour, Mohammed A Alsubai, Abdalla Eltoum Ali, Mohammad Fahad Ullah, Nizar H Saeedi, Fahad Daefalah Alenzi","doi":"10.1002/jcla.25147","DOIUrl":"https://doi.org/10.1002/jcla.25147","url":null,"abstract":"Objectives: To determine the association between IGF2BP2 polymorphisms and type 2 diabetes mellitus.Methods: This study involved 422 individuals, 214 diabetes mellitus cases, and 208 healthy controls. The PCR-RFLP technique was used to determine the genotype of the IGF2BP2 gene for the SNPs rs4402960 (G>T) and rs1470579 (A>C).Results: The results showed that the C allele of the rs1470579 variant and the T allele of the rs440960 variant were significantly associated with type 2 diabetes mellitus group (p = 0.0029 and 0.0001) as reported for both alleles, respectively. High frequencies of haplotypes TA and TC were observed in type 2 diabetes patients.Conclusion: The present study showed that IGF2BP2 rs1470579 and rs4402960 polymorphism were significantly associated with the increased risk of T2DM in the Saudi Arabian population and presented a genetic model to screen the high-risk individuals with further validations.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25147"},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Cryopreservation on T-Cell Subsets by Flow Cytometry Automated Algorithmic Analysis and Conventional Analysis.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-01-03 DOI: 10.1002/jcla.25146

Qian Min, Qiao Lv, Lu Jiang, Qian Chen, Jin Peng, Hongli Zhou, Ju Zhou, Qian Dai, Jianyun Zhou, Qing Huang

{"title":"The Effect of Cryopreservation on T-Cell Subsets by Flow Cytometry Automated Algorithmic Analysis and Conventional Analysis.","authors":"Qian Min, Qiao Lv, Lu Jiang, Qian Chen, Jin Peng, Hongli Zhou, Ju Zhou, Qian Dai, Jianyun Zhou, Qing Huang","doi":"10.1002/jcla.25146","DOIUrl":"https://doi.org/10.1002/jcla.25146","url":null,"abstract":"Background: Low-temperature cryopreservation is a common method for scientific research and clinical sample preservation when utilizing flow cytometry. In flow cytometry data analysis, traditional manual \"gating\" is susceptible to past experience and faces the challenge of manual subjective bias, time-consuming, and multidimensional data analysis. With the development of algorithms, the advantages of dimensionality reduction and clustering in result analysis are gradually becoming more prominent.Methods: Flow cytometry was used to detect the effects of cryopreservation and freeze-thaw cycle on T-cell subsets, and to analyze the data using automated algorithmic analysis and conventional manual \"gating\" methods.Results: The results showed that the number and viability of cells decreased slightly after one freeze-thaw within 2 weeks of cryopreservation, and there was no significant change in the subpopulation proportions and spatial locations by both analysis methods. The changes were significant with the increase of cryopreservation time and freeze-thaw cycle, which may be due to changes in the molecular conformation of the maker as a result of cryopreservation.Conclusion: The results indicate that both analysis methods have reached similar conclusions, but the repeatability and objectivity of automated algorithmic analysis have compensated for the uncertainty brought about by the subjective discretization of traditional manual \"gating.\" In addition, the automated algorithmic analysis more intuitively highlights the spatial positional variations in the relationships between cell populations.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25146"},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and Application of a Dual Immunoassay Method Based on ICP-MS for Stable Element Labeling Antibodies.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2024-12-31 DOI: 10.1002/jcla.25145

Xuyao Ji, Jing Wu, Jing Zhang, Wenxu Yang, Yu Liu

{"title":"Establishment and Application of a Dual Immunoassay Method Based on ICP-MS for Stable Element Labeling Antibodies.","authors":"Xuyao Ji, Jing Wu, Jing Zhang, Wenxu Yang, Yu Liu","doi":"10.1002/jcla.25145","DOIUrl":"https://doi.org/10.1002/jcla.25145","url":null,"abstract":"Background: To establish a dual immunoassay based on inductively coupled plasma mass spectrometry (ICP-MS) with stable element labeling antibodies for the simultaneous detection of alpha-fetoprotein (AFP) and prostate-specific antigen (PSA) in serum and evaluate its performance and clinical sample validation.Methods: The immunoassay system based on the double antibody sandwich method was established using magnetic beads as solid-phase carriers and rare earth elements europium (Eu) and samarium (Sm) as element tags. The test conditions were optimized. According to the Clinical Laboratory Standards Institute (CLSI) guidelines and the \"Guidelines for Performance Validation of Clinical Chemistry Quantitative Testing Procedures,\" performance validation was conducted using the established method, followed by clinical sample validation.Results: The LOD and AMR for AFP were 0.76 ng/mL and 1.5-1200 ng/mL, respectively; the LOD and AMR for PSA were 0.41 ng/ mL and 0.5-250 ng/ mL, respectively. The intra-batch precision for AFP and PSA was all less than 3.33%, and the inter-batch precision for AFP and PSA was all less than 4.97%. The recoveries of AFP and PSA were 95.08%-104.18% and 97.65%-100.72%, respectively. The correlation coefficients between the established and the CLIA methods were r = 0.9930 for AFP and r = 0.9852 for PSA.Conclusions: We have developed a dual immunoassay method based on ICP-MS, which can quantitatively detect AFP and PSA in serum within a single analysis. This method exhibits good precision, a wide linear range, and high specificity, meeting the requirements for clinical sample detection and holding potential for clinical application.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25145"},"PeriodicalIF":2.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of Candida albicans pRB895-SAP2-SC5314 With SAP2 High Expression and Its Effects on Adhesion.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2024-12-27 DOI: 10.1002/jcla.25144

Lan Xue, Lu Yang, Bingqian Zhao, Wenli Feng, Jing Yang, Yan Ma

{"title":"Construction of Candida albicans pRB895-SAP2-SC5314 With SAP2 High Expression and Its Effects on Adhesion.","authors":"Lan Xue, Lu Yang, Bingqian Zhao, Wenli Feng, Jing Yang, Yan Ma","doi":"10.1002/jcla.25144","DOIUrl":"https://doi.org/10.1002/jcla.25144","url":null,"abstract":"Background: SAP2 is closely associated with the pathogenicity and drug resistance of Candida albicans (C. albicans). Our study aimed to construct C. albicans with SAP2 overexpression (pRB895-SAP2-SC5314) to explore the influence of SAP2 on the adhesion of C. albicans and predict the interaction between magnolol and Sap2 by molecular docking.Methods: The recombinant plasmid pRB895-SAP2 with high SAP2 expression was prepared using a plasmid extraction kit and transformed into C. albicans strain SC5314 using an improved lithium acetate conversion method to construct PRB895-SAP2-SC5314. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect the expression of adhesion-related genes in the different strains. Molecular docking and visual analysis of magnolol and Sap2 were performed using the CB-DOCK2 platform.Results: Compared with the control SC5341 and SC5341 transfected with pRB895, SAP2 expression was significantly higher in the pRB895-SAP2-SC5314 strain (p < 0.05). Based on the sequencing and mapping results, the pRB895-SAP2-SC5314 strain was successfully prepared. SAP2 overexpression significantly downregulated ALS1 expression (p < 0.05), whereas ALS3, TEC1, HOG1, PHR1, and TUP1 expression was downregulated in C. albicans (p < 0.05). The optimal docking result for magnolol and Sap2 protein was -8.1 kcal/mol of vina score, which was considered good docking.Conclusions: SAP2 overexpression may strengthen the adhesion and pathogenicity of C. albicans, and magnolol may act as an Sap2 inhibitor that affects the adhesion of C. albicans.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25144"},"PeriodicalIF":2.6,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysosphingolipid Quantitation in Plasma and Dried-Blood Spots Using Targeted High-Resolution Mass Spectrometry.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2024-12-27 DOI: 10.1002/jcla.25131

Franklin Ducatez, Wladimir Mauhin, Jules Ottaviani, Thomas Plichet, Carine Pilon, Olivier Lidove, Fréderic Barbey, Régine Perrichot, Sabrina Vergnaud, Marc G Berger, Juliette Berger, Nadia Belmatoug, Yann Nadjar, Foudil Lamari, Esther Noel, Stéphane Marret, Soumeya Bekri, Abdellah Tebani

{"title":"Lysosphingolipid Quantitation in Plasma and Dried-Blood Spots Using Targeted High-Resolution Mass Spectrometry.","authors":"Franklin Ducatez, Wladimir Mauhin, Jules Ottaviani, Thomas Plichet, Carine Pilon, Olivier Lidove, Fréderic Barbey, Régine Perrichot, Sabrina Vergnaud, Marc G Berger, Juliette Berger, Nadia Belmatoug, Yann Nadjar, Foudil Lamari, Esther Noel, Stéphane Marret, Soumeya Bekri, Abdellah Tebani","doi":"10.1002/jcla.25131","DOIUrl":"https://doi.org/10.1002/jcla.25131","url":null,"abstract":"Background: Sphingolipidoses are rare inherited metabolic diseases belonging to lysosomal diseases. Early and accurate diagnosis is crucial for effective management and treatment. In this study, we aimed to develop a robust method to accelerate the diagnosis of these sphingolipidoses using dried blood spots and plasma.Method: We employed high-resolution mass spectrometry coupled with liquid chromatography (LC-HRMS) to analyze 6 lysosphingolipids (GlcSph/Psychosine, LysoGb3, LysoSM, LysoSM509, LysoGM1, and LysoGM2) on dried blood spots and plasma samples. The method was used to measure the lysosphingolipid levels in a group of 30 control subjects and 204 samples from patients with sphingolipidoses (61 dB and 143 plasma) including Fabry, Gaucher, GM2 Gangliodosis, Niemann-Pick type A/B, and Niemann-Pick type C.Results: The developed multiplex LC-HRMS method demonstrated linearity, precision, and quantification performances particularly for GlcSph/Psychosine and LysoGb3 on samples including controls and patients with sphingolipidoses. LysoSM showed recovery variability, wherease LysoGM1 and LysoGM2 showed higher matrix effect.Conclusion: Our study presents a high-resolution mass spectrometry method along with the established cutoff values, providing a valuable tool for targeted screening, accurate diagnosis, and monitoring sphingolipidoses. Furthermore, DBS showed reliable results that lay the path to a broader adoption for screening these diseases.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25131"},"PeriodicalIF":2.6,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Atherogenic Index of Plasma Associated With Delayed Graft Function in Living Donor Renal Transplant Recipients: A Single-Center Study in Vietnam.

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2024-12-24 DOI: 10.1002/jcla.25142

Dung Pham Thai, Ha Nguyen Thi Thu, Tue Nguyen Tri, Tiep Tran Dac, Kien Nguyen Trung, Dung Nguyen Huu, Trung Hoang Anh, Hinh Tran Van, Viet Thang Le

{"title":"High Atherogenic Index of Plasma Associated With Delayed Graft Function in Living Donor Renal Transplant Recipients: A Single-Center Study in Vietnam.","authors":"Dung Pham Thai, Ha Nguyen Thi Thu, Tue Nguyen Tri, Tiep Tran Dac, Kien Nguyen Trung, Dung Nguyen Huu, Trung Hoang Anh, Hinh Tran Van, Viet Thang Le","doi":"10.1002/jcla.25142","DOIUrl":"https://doi.org/10.1002/jcla.25142","url":null,"abstract":"Purpose: To determine the predictive value of the atherogenic index of plasma before transplant for delayed graft function.Patients and methods: A cross-sectional, longitudinal, non-interventional, non-controlled study of 167 patients undergoing kidney transplantation from living donors, with a mean age of 39.34 ± 11.86 years old, 53.3% male, and a pre-transplant hemodialysis time of 14 (7-36) months. Delayed graft function was defined as decreased blood creatinine < 25% within the first 24 h compared to pre-transplantation, and the patients needed hemodialysis in the first 7 days. The atherogenic index of plasma was calculated based on pre-transplant plasma triglycerides and high-density lipoprotein cholesterol concentrations.Results: The ratio of delayed graft function in renal transplant recipients from living donors was 13.8% (23/167 patients). Hemodialysis time, the ratio of hepatitis infection, overweight and obese, atherosclerosis, positive PRA, and acute rejection in the DGF (+) group were higher than those of the DGF (-) group, p < 0.05 and < 0.001. In particular, plasma CRP-hs level and AIP also were higher in DGF (+) patients compared to those of DGF (-) ones, p < 0.001. Long hemodialysis time, obesity, high plasma CRP-hs, and high AIP in pre-transplant patients were independent factors related to DGF and had predictive value for DGF after kidney transplantation, in which AIP had good predictive value: AUC = 0.859, p < 0.001.Conclusion: Delayed graft function was relatively common in renal transplant recipients from living donors. AIP before kidney transplant was a good predictor for delayed graft function.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e25142"},"PeriodicalIF":2.6,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0