Journal of Clinical Laboratory Analysis最新文献

{"title":"Intra-Laboratory and Inter-Laboratory Variations Analysis for HbA1c Assays in China: Using Internal Quality Control and External Quality Assessment Data","authors":"Yongyong Lou,&nbsp;Zhiming Shan,&nbsp;Qian Chen,&nbsp;Fengfeng Kang","doi":"10.1002/jcla.70036","DOIUrl":"https://doi.org/10.1002/jcla.70036","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>With the worldwide increase of diabetes mellitus prevalence, ensuring the performance of HbA1c assays is essential. Internal quality control (IQC) and external quality assessment (EQA) serve as critical components of quality assurance systems and provide comprehensive performance assessment. We aimed to evaluate the intra-laboratory and inter-laboratory variations of HbA1c assays using EQA and IQC data.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 326 laboratories continuously participating in the HbA1c EQA program from 2020 to 2023 were included, of which 168 laboratories reported IQC data voluntarily. Acceptance rates and bias were evaluated at three levels: per sample, per year, and per manufacturer. Intra-laboratory and inter-laboratory variations were assessed according to biological variation (BV) criteria and clinical guidelines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean acceptance rates for 20 EQA samples were 48.5%, 77.8%, 86.7% within optimum, desirable, minimum BV criteria. Annual average acceptance rates increased from 91.8% to 96.9% based on EQA criterion. The absolute manufacturer-specific bias varied from 0.02% to 4.1%. By 2023, the overall inter-laboratory variation significantly decreased to 2.1%–2.6%. The median intra-laboratory variations reduced from 1.6% to 1.4% at the low QC level and from 1.2% to 1.0% at the high QC level. 58.9% and 79.8% of laboratories achieved an intra-laboratory CV &lt; 1.5% for low and high QC levels, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both inter-laboratory and intra-laboratory variations of HbA1c measurement have significantly decreased over the years. However, the difference among manufacturers still exists, and ongoing efforts are required to fully comply with clinical guideline requirements.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Value of Serum Cytokeratin 18 for the Staging of Liver Inflammation and Fibrosis: A Meta-Analysis","authors":"Jinwen Chen,&nbsp;Jian Hu,&nbsp;Jialin Zhuang,&nbsp;Zhong Li,&nbsp;Se Peng,&nbsp;Xiaoting Huang,&nbsp;Jialing Zhuang","doi":"10.1002/jcla.70034","DOIUrl":"10.1002/jcla.70034","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aims</h3>\u0000 \u0000 <p>Accurate assessment of liver inflammation and fibrosis is of vital importance in the clinical management of patients with liver diseases. Our aim is to conduct a meta-analysis to evaluate the diagnostic accuracy of serum cytokeratin 18 (CK18) for staging of liver inflammation and fibrosis against a liver biopsy in adults.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We systematically searched articles from eight electronic databases. Two authors independently selected included studies, extracted data, and assessed quality. In our meta-analysis, we used the random-effects meta-analysis model. Publication bias, sensitivity analysis, heterogeneity analysis, and post-test probability were used in this meta study.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 20 studies with 2235 patients were initially found by the search strategies. The pooled sensitivity, specificity, and area under the curve (AUC) of the summary receiver operating characteristic curve were 0.56, 0.81, and 0.810 for significant fibrosis; 0.64, 0.76, and 0.785 for advanced fibrosis; 0.53, 0.76, and 0.830 for cirrhosis; and 0.68, 0.73, and 0.786 for significant inflammation, respectively. High heterogeneity was observed in our meta-analysis because of factors such as the proportion of males, total number, and antigens of CK-18.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Serum CK18 had moderate diagnostic value (AUC &gt; 0.7) in different stages of liver fibrosis and significant inflammation, offering a complementary approach to other non-invasive indicators such as serological biomarkers and imaging techniques. Future research should focus on elucidating the role of CK18 in the occurrence and progression of hepatitis and liver fibrosis, particularly in liver diseases with diverse etiologies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of COVID-19-Associated Candidemia Among Burn Patients.","authors":"Maryam Salimi, Javad Javidnia, Azam Moslemi, Mahdi Abastabar, Mohammad Reza Mobayen, Golnar Rahimzadeh, Nahid Mirzaei Tirabadi, Seyedehzahra Nouranibaladezaei, Hassan Asghari, Behnam Sobouti, Mostafa Dahmardehei, Seyedmojtaba Seyedmousavi, Tahereh Shokohi","doi":"10.1002/jcla.70031","DOIUrl":"https://doi.org/10.1002/jcla.70031","url":null,"abstract":"<p><strong>Background: </strong>The emergence of COVID-19 has led to a significant public health crisis, and an increase in fungal infections, including candidemia. Candida species are frequently found in intensive care units (ICUs), and it is a common cause of death in many patients. The isolates were identified using polymerase chain reaction-restriction. In this study, We investigated the factors linked to Candida infections in COVID-19 burn patients in the ICU and assessed the antifungal susceptibility of the isolates in vitro.</p><p><strong>Methods: </strong>Out of 335 burn patients admitted to the ICU, fifty-six with concurrent COVID-19 were included in this study. A total of 133 yeast isolates were obtained from burn wounds, 29 from blood cultures, and 36 from urine cultures. The isolates were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.</p><p><strong>Results: </strong>Out of fifty-six patients, twenty-nine had infections and forty-eight had colonization, with Candida parapsilosis being the most common species. Twenty-one patients died during their ICU stay, with mortality rates of 43.8% among colonized patients and 69.0% among infected patients. Fluconazole and itraconazole exhibited the highest minimum inhibitory concentrations, while luliconazole and amphotericin B were identified as the most effective antifungal agents.</p><p><strong>Conclusion: </strong>Our findings indicate that colonization may act as an important prognostic factor prior to the onset of candidemia. In addition, prolonged hospitalization, catheter use, and concurrent COVID-19 infection were identified as key risk factors for candidemia in this patient group. Notably, the rising drug resistance in non-albicans Candida species is a major public health concern.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70031"},"PeriodicalIF":2.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosomal Microarray Analysis and Karyotype Analysis for Prenatal Diagnosis of Fetuses With Abnormal Ultrasound Soft Markers.","authors":"Lina Liu, Lingna She, Zhiyuan Zheng, Shuxian Huang, Heming Wu","doi":"10.1002/jcla.70033","DOIUrl":"https://doi.org/10.1002/jcla.70033","url":null,"abstract":"<p><strong>Objective: </strong>To explore and evaluate the value of chromosomal microarray analysis (CMA) in fetuses with abnormal ultrasound soft markers.</p><p><strong>Methods: </strong>A retrospective study was conducted on 193 fetuses with abnormal ultrasound soft markers who received prenatal diagnosis at Meizhou People's Hospital, between October 2022 and February 2024. Genetic detection of fetal specimens obtained by ultrasound-guided puncture was carried out. The detection rates of karyotype analysis and CMA for chromosomal abnormalities in different ultrasonic abnormalities were analyzed.</p><p><strong>Results: </strong>Of the 193 fetuses, there were 77 (39.9%) fetuses with increased nuchal translucency(NT) thickness, 33 (17.1%) with ventriculomegaly, 29 (15.0%) with nasal bone hypoplasia, followed by choroid plexus cyst, pyelic separation, echogenic bowel, single umbilical artery, with persistent left superior vena cava, and persistent right umbilical vein. Aneuploidy was mainly found in fetuses with increased NT thickness or and nasal bone hypoplasia, while P/LP CNVs were mainly concentrated in fetuses with increased NT thickness or ventriculomegaly. The detection rate of karyotype was 5.7% (11/193), the detection rate of aneuploidy plus P/LP CNVs in fetuses with abnormal ultrasonic soft markers by CMA was 10.9% (21/193), and the additional detection rate of CMA was 5.2%.</p><p><strong>Conclusions: </strong>CMA can significantly improve the detection rate of chromosomal abnormalities in fetuses with abnormal ultrasonic soft markers compared with karyotype analysis. There was a significant difference in detection rates of chromosomal abnormality between CMA and karyotype analysis in the single ultrasonic abnormality group, but none in the multiple ultrasonic abnormalities group.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70033"},"PeriodicalIF":2.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordination of IFT20 With Other IFT Components Is Required for Ciliogenesis.","authors":"Weishu Wang, Ying Shan, Ruming Liu, Dengwen Li, Jun Zhou, Quanlong Lu, Huijie Zhao","doi":"10.1002/jcla.70000","DOIUrl":"https://doi.org/10.1002/jcla.70000","url":null,"abstract":"<p><strong>Background: </strong>Primary cilia are organelles formed on the cell surface. They can act as cellular antennae to sense signals and play important roles in various biological processes. Abnormalities in primary cilia lead to a variety of diseases collectively known as ciliopathies. Intraflagellar transport protein 20 (IFT20) has been implicated in ciliogenesis.</p><p><strong>Methods: </strong>IFT20 knockout cell lines were established using the CRISPR-Cas9 gene editing technology. The GFP-IFT20 plasmid was constructed with the Gateway cloning system. Protein levels were detected via immunoblotting, and the localization of IFT20, acetylated α-tubulin, ARL13B, CP110, MKS3, IFT88, and IFT140 in wild-type and IFT20 knockout cells was examined by immunofluorescence microscopy. The fluorescence intensities were analyzed using ImageJ. Data quantifications and mass spectrometry results were analyzed using GraphPad Prism and Metascape.</p><p><strong>Results: </strong>The IFT20 deficiency impaired ciliogenesis and reduced cilium length. IFT20 depletion did not affect the removal of centriolar coiled-coil protein 110 (CP110) from the mother centriole or the recruitment of Meckel-Gruber syndrome type 3 (MKS3) to the transition zone. Mass spectrometry analysis revealed that proteins interacting with IFT20 were mainly IFT components. IFT20 knockout decreased the levels of both IFT88 and IFT140, and abrogated IFT88 localization at the basal body and ciliary axoneme. IFT20 knockout also impaired IFT140 localization at the ciliary axoneme but did not affect its localization at the basal body.</p><p><strong>Conclusions: </strong>IFT20 is involved in ciliogenesis by regulating the level and localization of other IFT proteins and may have important implications in ciliopathies and related diseases.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70000"},"PeriodicalIF":2.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergency Department Serum Electrolyte Patterns in Paroxysmal Atrial Fibrillation","authors":"Zahra Hooshanginezhad,&nbsp;Sepehr Nemati,&nbsp;Mehdi Rezaee,&nbsp;Shahin Keshtkar Rajabi","doi":"10.1002/jcla.70030","DOIUrl":"10.1002/jcla.70030","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Little is known about the relationship between circulating electrolyte concentrations and paroxysmal atrial fibrillation in the emergency department. We aimed to characterize circulating electrolyte concentrations in patients with paroxysmal atrial fibrillation compared with those of nonspecific control patients admitted to the emergency department.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In total, data from 520 individuals with paroxysmal atrial fibrillation and 1,040 randomly selected 1040 patients without atrial fibrillation (1:2 ratio), all admitted to the emergency department (January 2010–December 2015), were analyzed. A classification model was developed using a tree-based machine learning algorithm, and the importance of variables was measured.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Patient age, serum glucose, sodium, potassium, calcium, phosphate, and sex were significantly associated with paroxysmal atrial fibrillation (all <i>p</i> &lt; 0.001). For serum magnesium, the difference approached significance (<i>p</i> = 0.096). The model had a moderate performance with a 10-fold cross-validation accuracy of 0.728 and a sensitivity, specificity, area under the curve, and likelihood ratio of 0.613, 0.770, 0.692, and 2.67, respectively. Overall, age and glucose were the most important variables followed by serum sodium, potassium, and calcium. Male sex, older age, and a higher serum sodium, calcium, potassium, and magnesium, and a lower serum glucose and phosphate were associated with a higher likelihood of paroxysmal atrial fibrillation in the emergency department.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Serum electrolyte imbalances, particularly in sodium, potassium, and magnesium, are significantly associated with paroxysmal atrial fibrillation in emergency settings. Emergency physicians should monitor and correct these electrolytes to improve early PAF management and potentially prevent adverse outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Lactate Accumulation via USP38-Mediated MCT1 Deubiquitination Activates AKT/mTOR Signaling to Mitigate PM2.5-Induced Lung Injury","authors":"Zixiao Chen,&nbsp;Jing Cao,&nbsp;Shujie Hou,&nbsp;Lingshan Chao,&nbsp;Jingwen Li,&nbsp;Zaixing Jia,&nbsp;Siqin Han,&nbsp;Jialun Chen,&nbsp;Xixin Yan","doi":"10.1002/jcla.70028","DOIUrl":"10.1002/jcla.70028","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Lactate, traditionally viewed as a glycolysis byproduct, has emerged as an important mediator influencing immunity, inflammation, and tissue damage. While PM2.5 exposure is known to cause various metabolic disturbances, the role of lactate metabolism in PM2.5-induced lung injury remains unclear. This study aims to elucidate the mechanisms underlying PM2.5-induced lung injury from a metabolic perspective.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Lactate and pyruvate assays were performed to assess metabolic changes following PM2.5 exposure. Protein expression and tissue damage were assessed using Western blot, IHC, ELISA, and TUNEL staining. The biological role of USP38 in PM2.5-induced injury was identified using gain- and loss-of-function experiments. Co-immunoprecipitation and ubiquitination assays were conducted to analyze the interaction between USP38 and MCT1, as well as the regulation of MCT1 deubiquitination. The role of MCT1 in lactate metabolism and PM2.5-induced apoptosis was validated through gene editing. Proteomics revealed the potential mechanisms involved in USP38 regulation of apoptosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our results demonstrated that PM2.5 exposure induced lactate accumulation, leading to cell apoptosis and lung injury. USP38 stabilized MCT1 expression by deubiquitination, facilitating lactate export and reducing apoptosis and lung injury caused by lactate accumulation. Mechanistically, PM2.5 increased lactate production, suppressed AKT/mTOR pathway activation, and promoted apoptosis and lung injury. USP38 promoted lactate export through MCT1, activated the AKT/mTOR pathway, and mitigated PM2.5-induced lung injury.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>USP38 reduces lactate accumulation by promoting AKT/mTOR pathway activation through MCT1-mediated lactate export, thereby alleviating PM2.5-induced lung injury. These findings reveal a novel mechanism of PM2.5-related lung injury and highlight potential therapeutic targets.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"microRNA-Mediated Regulation of Oxidative Stress in Cardiovascular Diseases.","authors":"Sakhavat Abolhasani, Yasin Ahmadi, Davood Fattahi, Yavar Rostami, Khalil Maleki Chollou","doi":"10.1002/jcla.70017","DOIUrl":"https://doi.org/10.1002/jcla.70017","url":null,"abstract":"<p><strong>Background: </strong>Cardiovascular diseases (CVDs) are the leading cause of mortality globally, often linked to oxidative stress. MicroRNAs (miRNAs) have emerged as significant regulators of oxidative stress within the cardiovascular system.</p><p><strong>Objective: </strong>This review examines the complex relationship between miRNAs and oxidative stress, clarifying their effects on gene expression pathways related to ROS production and detoxification in CVDs.</p><p><strong>Methods: </strong>From August to October 2024, we conducted a comprehensive search of PubMed, Scopus, Web of Science, and Google Scholar for studies published between 2014 and 2024 investigating the role of miRNAs in oxidative stress and cardiovascular diseases.</p><p><strong>Results: </strong>Specific miRNAs have been identified as critical regulators in the pathophysiology of CVDs, with distinct expression patterns correlated with conditions such as hypertension, coronary artery disease, and heart failure. For instance, miR-21 exacerbates oxidative stress by targeting genes essential for redox homeostasis, while miR-210 promotes endothelial cell survival under hypoxic conditions by mitigating ROS levels.</p><p><strong>Conclusion: </strong>The reciprocal relationship between miRNAs and oxidative stress highlights the potential for therapeutic interventions targeting miRNA expression and activity in managing CVDs. Understanding these molecular mechanisms is vital for developing innovative strategies to address oxidative damage in cardiac tissues and improve cardiovascular health outcomes.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70017"},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble Triggering Receptors on Myeloid Cells-1 Could Be a Potential Biomarker for Disease Activity in Familial Mediterranean Fever","authors":"Meryem Cemiloglu,&nbsp;Oguzhan Ozcan,&nbsp;Gezmis Kimyon,&nbsp;Abdullah Arpaci,&nbsp;Hamdi Oguzman","doi":"10.1002/jcla.70027","DOIUrl":"10.1002/jcla.70027","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Familial Mediterranean Fever (FMF) is an autoinflammatory disease. We aimed to investigate serum soluble Triggering Receptor Expressed on Myeloid Cells-1 and 2 (sTREM-1 and sTREM-2) levels in patients with FMF during both attack and attack-free periods and their relationship with disease activity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Fifty-seven FMF patients, 27 in the attack and 30 in the attack-free period, along with 30 age- and sex-matched healthy controls, were enrolled in the study. Demographic and clinical data were obtained from hospital records, and the disease severity scores (DSS) were calculated. Serum levels of sTREM-1, sTREM-2, TNF-α, and IL-1β were assayed by ELISA. CRP levels were measured by the nephelometric method. Receiver operating characteristic (ROC) analysis was performed for sTREM-1 levels to detect attacks in patients with FMF.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>sTREM-1 levels were significantly higher in the attack group than in the attack-free and control groups (<i>p</i> &lt; 0.001). IL-1β levels were elevated in FMF patients (<i>p</i> = 0.003), and CRP levels differed significantly among the groups (<i>p</i> &lt; 0.001). No significant differences in sTREM-2 or TNF-α were observed. In the FMF-attack group, sTREM-1 positively correlated with TNF-α (<i>r</i> = 0.526, <i>p</i> = 0.005), IL-1β (<i>r</i> = 0.475, <i>p</i> = 0.014), CRP, and fibrinogen (<i>p</i> &lt; 0.001). The DSS was significantly correlated with sTREM-1 and CRP levels in FMF patients (respectively, <i>r</i> = 0.270, <i>p</i> = 0.042; <i>r</i> = 0.292, <i>p</i> = 0.027). The area under the curve (AUC) was 0.807 (95% CI, 0.695–0.92, <i>p</i> &lt; 0.001), with an optimal sTREM-1 cutoff of 232 pg/mL to detect attacks.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>sTREM-1 may play a critical role in the inflammatory response in FMF disease and could serve as a potential marker for assessing disease activity.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Causal Relationship Between Immune Cells and Idiopathic Pulmonary Fibrosis: A Mendelian Randomization Analysis","authors":"Peng Gong,&nbsp;Yimin Lu,&nbsp;Xi Chai,&nbsp;Xiaobo Li","doi":"10.1002/jcla.70026","DOIUrl":"10.1002/jcla.70026","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Idiopathic pulmonary fibrosis (IPF) is a progressive and irreversible interstitial lung disease with a complex pathogenesis involving multiple immune cells. This study investigates the relationship between immune cells and IPF using Mendelian randomization (MR) analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A two-sample MR analysis was performed using genome-wide association studies (GWAS) and immune cell databases by R software. We analyzed data from 1028 European individuals with IPF, focusing on 731 immune traits. The primary method of analysis was inverse variance weighting (IVW), supplemented with sensitivity analyses, including MR-Egger regression and MR-PRESSO, to detect and correct for pleiotropy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The MR analysis identified six immune panels and 23 immune traits significantly associated with IPF, including five traits that increase and 18 traits that decrease IPF risk. Notable traits increasing IPF risk included switched memory B-cells (OR = 1.27, <i>p</i> = 0.0029) and IgD- CD38dim B-cells (OR = 1.08, <i>p</i> = 0.0449). Traits associated with a reduced IPF risk included central memory CD4+ T-cells (%CD4+, OR = 0.96, <i>p</i> = 0.0489), CD20 on naive-mature B-cells (OR = 0.94, <i>p</i> = 0.0499), and CD33br HLA-DR+ absolute count (AC) (OR = 0.93, <i>p</i> = 0.0489). There was no significant causal relationship between IPF disease and some immune traits (<i>p</i> &gt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study suggests a potential causal link between specific immune cell traits and the development of IPF, providing new insights into the disease's immunological mechanisms. Future research should focus on validating these findings in larger, more diverse populations to inform drug development and therapeutic strategies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}