Journal of Clinical Laboratory Analysis最新文献

{"title":"Assessment of Factors Contributing to Infection Severity and High Levels of Drug Resistance in Clinical Enterococcus Isolates.","authors":"Maryam Koleini, Ahmad Mosadegh, Farzan Madadizadeh, Hamid Heidari","doi":"10.1002/jcla.70063","DOIUrl":"https://doi.org/10.1002/jcla.70063","url":null,"abstract":"<p><strong>Background: </strong>Various factors, including virulence determinants, biofilm formation, and antimicrobial resistance, contribute to the severity of infections caused by Enterococcus spp.</p><p><strong>Methods: </strong>Enterococcus isolates were obtained from hospitalized patients in Yazd, Iran, and identified using microbiological and molecular tests. High-level resistance, biofilm formation, and the genes encoding virulence factors and resistance were investigated following standard methods.</p><p><strong>Results: </strong>Enterococcus faecalis was the most prevalent species (60.7%), followed by Enterococcus faecium (30.4%). Linezolid was highly effective, with 94.6% of isolates being susceptible. However, more than 76% of isolates exhibited resistance to rifampin, erythromycin, tetracycline, and ciprofloxacin, and 94.6% were multidrug-resistant (MDR). Additionally, 39.3% of the isolates were vancomycin-resistant enterococci (VRE) with a MIC > 32 μg/mL, and the vanA gene was detected in 35.7% of the isolates. High-level resistance to gentamicin and streptomycin was seen in 60.7% and 50% of the isolates, respectively. The most prevalent aminoglycoside resistance gene was aph(3')-IIIa (62.5%) followed by ant(6')-Ia (58.9%), and aac(6')-Ie-aph(2″)-Ia (50%). The ant(3″)-Ia was found in only one isolate. Most of the isolates (87.5%) were biofilm producers, and the distribution of virulence-encoding genes was as follows: gelE (66.1%), efaA (57.1%), asa1 (51.8%), esp (25%), cylA (19.6%), and hyl (8.9%). Furthermore, the ace gene was present in 79.4% of E. faecalis isolates, while the fnm and acm genes were found in 76.5% and 23.5% of E. faecium isolates, respectively.</p><p><strong>Conclusion: </strong>The study highlights the significant role of notable drug resistance and the widespread presence of virulence traits in the development of enterococcal infections.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70063"},"PeriodicalIF":2.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference Intervals of Hematological Parameters Among Healthy Adults in Northern Sudan: A Community-Based Cross-Sectional Study.","authors":"Mohamed F Lutfi, Ahmed Ali Hassan, Ishag Adam","doi":"10.1002/jcla.70061","DOIUrl":"https://doi.org/10.1002/jcla.70061","url":null,"abstract":"<p><strong>Background: </strong>Recent studies from various countries, including those in Africa, have highlighted the importance of region-specific data in assessing hematological health. However, no study has established hematological reference intervals (RIs) in Northern Sudan. We aimed to investigate the normal hematological values among apparently healthy adults in Northern Sudan.</p><p><strong>Methods: </strong>A cross-sectional study was conducted in Northern Sudan. Standardized procedures measured participants' weight, height, and hematological parameters. The Mann-Whitney U test was used to compare the parameters between males and females. Mean, median, and RIs (2.5th-97.5th percentiles) were computed for the adults.</p><p><strong>Results: </strong>Two hundred fifty-three adults were enrolled (141 [55.7%] males and 112 [44.3%] females). The median (interquartile [IQR]) of the enrolled adults' age was 40.0 (29.7-50.0) years. The RIs for both genders, hemoglobin 9.71-16.10 g/dL, red blood cells (RBC) 3.66-5.79 × 10⁶/mm³, hematocrit (HCT) 28.02%-46.75%, mean corpuscular volume (MCV) 64.02-95.65 fL, mean corpuscular hemoglobin (MCH) 21.57-33.80 pg, mean corpuscular hemoglobin concentration (MCHC) 31.60-36.56 g/dL, platelet count 155.35-454.30 × 10³/mm³, and total white blood cells (WBCs) 2.90-10.76 × 10³/mm³. Significantly higher median values were observed in males compared to females for hemoglobin, RBC, HCT, MCH, MCHC, and platelet distribution width. In contrast, females demonstrated significantly higher red cell distribution width-coefficient of variation, WBC, platelet, mean platelet volume, and plateletcrit than males. MCV showed no significant difference between genders.</p><p><strong>Conclusion: </strong>The study's findings underscore the importance of establishing RIs for each region and specific gender population. RIs should also be established in other regions of Sudan to enhance clinical relevance and accuracy.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70061"},"PeriodicalIF":2.6,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introducing Two Novel Indices for Evaluating Coronary Artery Stenosis: Athero-Inflammatory Index and Athero-Inflammatory Glucose Index.","authors":"Rana Kolahi Ahari, Mohsen Moohebati, Mohammad Tajfard, Habibollah Esmaily, Gordon Ferns, Majid Ghayour-Mobarhan, Sara Saffar Soflaei","doi":"10.1002/jcla.70050","DOIUrl":"https://doi.org/10.1002/jcla.70050","url":null,"abstract":"<p><strong>Background: </strong>We investigated the association of two novel indices, the athero-inflammatory (AI) index and athero-inflammatory glucose (AIG) index, with coronary artery stenosis (CAS).</p><p><strong>Methods: </strong>In this case-control study, the cases were grouped as angiography (+) and angiography (-) according to the angiographic results. The control group comprised subjects who attended clinics for routine check-ups or pre-employment medical assessments. The AI index and AIG index were compared between the groups using ANOVA. Binary logistic regression (LR) was performed to find the association of the indices with angiography (+). Receiver operating characteristic (ROC) curve analysis was used to establish the cut-off values in differentiating angiography (+) from angiography (-) and healthy subjects. p < 0.05 were considered statistically significant.</p><p><strong>Results: </strong>Among a total of 2326 participants (761 angiography (+), 406 angiography (-), and 1159 controls), the AI index and AIG index were significantly different between the groups (p < 0.001). In LR analysis, after adjustment for potential confounders, the AI index and AIG index were independently associated with angiography (+). ROC curve analysis showed that the AI index (AUC: 0.895; 95% CI: 0.880, 0.908; p < 0.0001) and AIG index (AUC: 0.918; 95% CI: 0.905, 0.930; p < 0.0001) performed better diagnostic performance in differentiating angiography (+) from healthy subjects.</p><p><strong>Conclusion: </strong>AI index demonstrated higher AUC compared to other biomarkers in differentiating angiography (+) from angiography (-) and healthy subjects. If it combines with fasting glucose (AIG index), it is a promising indicator for the identification of the CAS particularly from a healthy population, with a promising AUC.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70050"},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applying the National Genomic DNA Reference Materials to Evaluate the Performance of Nanopore Sequencing in Identifying Thalassemia Variants.","authors":"Xingyu Wei, Xu Yang, Wanqing Han, Li Zhang, Guojun Ouyang, Shoufang Qu, Fang Yang, Xuexi Yang","doi":"10.1002/jcla.70044","DOIUrl":"https://doi.org/10.1002/jcla.70044","url":null,"abstract":"<p><strong>Objectives: </strong>Nanopore sequencing shows advantages in detecting single nucleotide variations (SNVs), deletions, and complex structural variants as a single test in thalassemia. However, the performance evaluation or verification of this method remains unestablished, which is essential before clinical utility and panel registration. Here, we developed a classification method for thalassemia mutations, enabling automated interpretation, visual representation, and identification of diverse mutation types.</p><p><strong>Methods: </strong>We used a total of 36 samples, comprising 32 reference materials and four clinical samples to assess the performance of nanopore sequencing in identifying variants in terms of concordance, precision, and the lower limits of detection.</p><p><strong>Results: </strong>Our analysis successfully identified 19 SNVs, six deletions, and two triplications using nanopore sequencing across all samples. Notably, these variants showed complete concordance of 100% with the genotypes of the reference materials and known results. The precision of nanopore sequencing for detecting thalassemia variants was consistently high, with neither false positive nor false negative observed. Furthermore, the lower limits of detection achieved in our study were 3 ng/μL.</p><p><strong>Conclusions: </strong>Overall, our study proved that the reference materials can be used to evaluate the performance of nanopore sequencing in identifying thalassemia mutations, and it is necessary to incorporate triplications when utilizing reference materials for performance evaluation of long-read sequencing. The consistent and robust performance of nanopore sequencing in this study demonstrates its potential as a reliable method for comprehensive variant detection in thalassemia and other genetic diseases diagnosis.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70044"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Risk Factors for Augmented Renal Clearance in Neurocritical Ill Patients.","authors":"Xiangru Ye, Qiang Yuan, Zhuoying Du, Haijun Yao, Lei Yang, Biwu Wu, Gang Wu, Weilin Shi, Yufeng Jin, Zhiping Liu, Caihua Xi, Jin Hu","doi":"10.1002/jcla.70047","DOIUrl":"https://doi.org/10.1002/jcla.70047","url":null,"abstract":"<p><strong>Background: </strong>Augmented renal clearance (ARC) refers to a phenomenon in critically ill patients characterized by increased creatinine clearance. Neurological patients seem to be at higher risk compared with other groups. The epidemiology study of ARC reported in critically ill neurological patients varies substantially with the definitions used and the population evaluated.</p><p><strong>Objective: </strong>We aimed to describe the prevalence of ARC and to explore risk factors in critically ill neurological patients.</p><p><strong>Methods: </strong>A retrospective observational study was conducted in a university-affiliated neurocritical care unit (NCCU). Study participants had a serum creatinine concentration (Scr) < 120 μmol/L. Kidney function was assessed by the 24-h creatinine clearance (CL_cr); ARC was defined as CLcr ≥ 120 mL/min/1.73m² in women and ≥ 130 mL/min/1.73m² in men. The prevalence and clinical characteristics of ARC were evaluated. Multivariate logistic regression analysis was used to assess variables associated with ARC occurrence.</p><p><strong>Results: </strong>Of the 137 patients, 56.2% were male, and the mean age was 50.2 (17.4) years. ARC was present in 55.5% of the NCCU patients, ranging from 50% in intracranial infection to 75% in patients with spinal lesions. ARC patients have a mean CL_cr159.3 (IQR:139.6-185.2) ml/min/1.73m². Age was the only factor independently associated with ARC (OR 0.996, 95% CI: 0.934-0.999, p = 0.043) in multivariable logistic analysis. Scr (Pearson correlation = -0.477) and cystatin C (Pearson correlation = -0.336) were found to have a negative correlation with ARC with statistically significant effects.</p><p><strong>Conclusion: </strong>ARC is prevalent in critically neurological patients. Age is likely to significantly influence renal clearance in this population, especially as patients with low Scr and cystatin C levels should be given more attention.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70047"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assisting the Diagnosis of Cirrhosis in Chronic Hepatitis C Patients Based on Machine Learning Algorithms: A Novel Non-Invasive Approach.","authors":"Emre Dirican, Tayibe Bal, Yusuf Onlen, Figen Sarigul, Ulku User, Nagehan Didem Sari, Behice Kurtaran, Ebubekir Senates, Alper Gunduz, Esra Zerdali, Hasan Karsen, Ayse Batirel, Ridvan Karaali, Hatice Rahmet Guner, Tansu Yamazhan, Sukran Kose, Nurettin Erben, Nevin Koc Ince, Iftihar Koksal, Nefise Oztoprak, Gulsen Yoruk, Suheyla Komur, Sibel Yildiz Kaya, Ilkay Bozkurt, Ozgur Gunal, Ilknur Esen Yildiz, Dilara Inan, Sener Barut, Mustafa Namiduru, Selma Tosun, Kamuran Turker, Alper Sener, Kenan Hizel, Nurcan Baykam, Fazilet Duygu, Hurrem Bodur, Guray Can, Hanefi Cem Gul, Ayse Sagmak Tartar, Guven Celebi, Mahmut Sunnetcioglu, Oguz Karabay, Hayat Kumbasar Karaosmanoglu, Fatma Sirmatel, Omer Fehmi Tabak","doi":"10.1002/jcla.70054","DOIUrl":"https://doi.org/10.1002/jcla.70054","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to determine the important features and cut-off values after demonstrating the detectability of cirrhosis using routine laboratory test results of chronic hepatitis C (CHC) patients in machine learning (ML) algorithms.</p><p><strong>Methods: </strong>This retrospective multicenter (37 referral centers) study included the data obtained from the Hepatitis C Turkey registry of 1164 patients with biopsy-proven CHC. Three different ML algorithms were used to classify the presence/absence of cirrhosis with the determined features.</p><p><strong>Results: </strong>The highest performance in the prediction of cirrhosis (Accuracy = 0.89, AUC = 0.87) was obtained from the Random Forest (RF) method. The five most important features that contributed to the classification were platelet, αlpha-feto protein (AFP), age, gamma-glutamyl transferase (GGT), and prothrombin time (PT). The cut-off values of these features were obtained as platelet < 182.000/mm³, AFP > 5.49 ng/mL, age > 52 years, GGT > 39.9 U/L, and PT > 12.35 s. Using cut-off values, the risk coefficients were AOR = 4.82 for platelet, AOR = 3.49 for AFP, AOR = 4.32 for age, AOR = 3.04 for GGT, and AOR = 2.20 for PT.</p><p><strong>Conclusion: </strong>These findings indicated that the RF-based ML algorithm could classify cirrhosis with high accuracy. Thus, crucial features and cut-off values for physicians in the detection of cirrhosis were determined. In addition, although AFP is not included in non-invasive indexes, it had a remarkable contribution in predicting cirrhosis.</p><p><strong>Trial registration: </strong>Clinicaltrials.gov identifier: NCT03145844.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70054"},"PeriodicalIF":2.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mid-Regional Proadrenomedullin Can Be Reliably Measured in Cerebrospinal Fluid to Improve Diagnosis of Central Nervous System Diseases.","authors":"Matteo Furlani, Daniela Visentini, Anna Rosa Cussigh, Fiorenza Pesente, Francesco Janes, Carlo Tascini, Francesco Curcio, Martina Fabris","doi":"10.1002/jcla.70058","DOIUrl":"https://doi.org/10.1002/jcla.70058","url":null,"abstract":"<p><strong>Background: </strong>Adrenomedullin (ADM) is a potent hormone-like peptide rapidly induced by hypoxia and inflammatory cytokines in the early stages of sepsis. For this reason, the dosage of its more stable precursor fragment called mid-regional (MR)-proADM is currently recommended to assist in triaging patients in the emergency department. Since MR-proADM dosage is currently only approved for use in plasma, we validated its dosage in cerebrospinal fluid (CSF) samples to improve the diagnosis of central nervous system (CNS) diseases.</p><p><strong>Methods: </strong>MR-proADM concentrations were measured in samples using a fully automated platform (Brahms Kryptor Gold Analyzer, Thermo Scientific, Germany), applying the same analytical conditions in plasma and CSF samples, to finally set up an accurate laboratory protocol to validate its dosage in CSF.</p><p><strong>Results: </strong>MR-proADM is highly stable in CSF samples stored at room temperature for up to 48 h, allowing it to be measured with confidence also in CSF samples that may be left on the bench for several hours. In addition, the repeatability and within-laboratory precision of the MR-proADM assay using CSF samples appeared equal to or better than those obtained by the manufacturer using plasma samples, allowing the use of this assay, with high precision, also for CSF samples.</p><p><strong>Conclusion: </strong>The reliable measure of MR-proADM in CSF and the role of this molecule in CNS will allow its introduction in the diagnostic process of infectious, inflammatory, and degenerative neurological diseases.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70058"},"PeriodicalIF":2.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of UCMA With Cartilage Pathogenesis and Inflammation in Patients With Rheumatoid Arthritis: A Novel Biomarker.","authors":"Münteha Nur Sonuç Karaboğa, Hamza Malik Okuyan, Serdar Dogan, Hamdi Oguzman, Gezmiş Kimyon","doi":"10.1002/jcla.70057","DOIUrl":"https://doi.org/10.1002/jcla.70057","url":null,"abstract":"<p><strong>Background: </strong>Rheumatoid arthritis (RA) is a persistent autoimmune disorder that impacts the joints, leading to a reduction in physical function and a decline in overall well-being. UCMA is a vitamin K-dependent protein that plays a role in some human diseases, including osteoarthritis and cardiovascular disorders. Nevertheless, the possible role of UCMA in the pathogenesis of RA remains unclear. Therefore, we aimed to investigate the expression of UCMA in serum samples of patients with RA, its relationship with disease activity and some blood markers.</p><p><strong>Methods: </strong>The current study included 98 RA patients and 24 healthy individuals. Serum UCMA, COMP, TNF-α, and IL-6 levels were measured using enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>Serum UCMA, COMP, TNF-α, and IL-6 expressions were significantly increased in RA patients compared to healthy controls (p < 0.05). The ROC curve analysis demonstrated that blood UCMA, COMP, TNF-α, and IL-6 levels had the capability to differentiate between patients with RA and healthy controls (p < 0.05). UCMA was positively correlated with certain laboratory indicators, such as COMP, TNF-α, IL-6, CRP, and CCP.</p><p><strong>Conclusion: </strong>Here, we report for the first time that UCMA may reflect cartilage degeneration and inflammatory changes in RA patients. Furthermore, UCMA could be employed as a predictive or diagnostic marker in the clinical practice of RA.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70057"},"PeriodicalIF":2.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression Profile of Serum CircFUNDC1 and CircUHRF1 Can Differentiate Between Colorectal Cancer and Inflammatory Bowel Diseases (Ulcerative Colitis and Crohn's Disease).","authors":"Marwa A Ali, Olfat G Shaker, Eman M Ezzat, Abeer A Khalefa, Naglaa A Ahmed, Raghda Yahia Abu El-Ela, Amal A Ibrahim Amin, Fatma Abdel Hamid Mohamed, Marwa Mamdouh Ahmed Abdelhafeez, Mohamed Saad Zaghloul Ahmed, Marwa N AbdelHafez, Abdulrhman Khaled Al Abdulqader, Reham Fares","doi":"10.1002/jcla.70039","DOIUrl":"https://doi.org/10.1002/jcla.70039","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is a worldwide burden. Circular RNAs are promising biomarkers for diagnosing and prognosis of CRC.</p><p><strong>Objective: </strong>To investigate the possible association of sera levels of CircFUNDC1 and CircUHRF1 expression with predisposition and clinicopathological findings in CRC, ulcerative colitis (UC), and Crohn's disease (CD) in Egyptian patients.</p><p><strong>Methods: </strong>The serum levels of CircFUNDC1 and CircUHRF1 were evaluated in 113 Egyptian subjects divided into four groups; CRC (31), UC (26), and CD (25) and compared to healthy controls (31) using quantitative polymerase chain reaction.</p><p><strong>Results: </strong>The median values of log2 serum fold change (FC) of CircFUNDC1 in CRC, UC, and CD patients were 9.11, 6.58, and 6.17, respectively. It was upregulated in all case groups. CRC, UC, and CD patients had significantly higher serum CircFUNDC1 levels than controls (p < 0.001). However, there were no significant differences among patient groups (CRC, UC, and CD). The medians of log 2 of serum FC CircUHRF1 in patients with CRC, UC, and CD were -2.00, 3.33, and 3.12, respectively. The CircUHRF1 serum level was lower in the CRC group of patients, with no significant difference between the CRC group and the controls. Serum CircUHRF1 was significantly overexpressed in patients with UC and CD compared to the CRC groups or controls (p < 0.001). By Roc curve analysis, both genes can differentiate CRC patients from inflammatory bowel disease (IBD) patients or healthy controls with p < 0.05.</p><p><strong>Conclusion: </strong>Serum CircFUNDC1 is a biomarker for CRC, while CircUHRF1 is a biomarker of IBD.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70039"},"PeriodicalIF":2.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Preliminary Association Study of H19 Non-Coding Gene Variants With Risk of Non-Hodgkin Lymphoma: A Case-Control Study and Computational Analysis.","authors":"Sara Kashani, Hoseinali Sasan, Behrouz Mollashahi, Gholamreza Bahari, Seyed Mahdi Hashemi, Mohsen Taheri","doi":"10.1002/jcla.70024","DOIUrl":"https://doi.org/10.1002/jcla.70024","url":null,"abstract":"<p><strong>Background: </strong>Non-Hodgkin lymphoma (NHL) is one of the most prevalent disorders worldwide, with a variety range of etiology from environmental to genetic factors. H19 is a non-coding RNA that codes no protein while playing regulatory roles and is hypothesized to be involved in susceptibility to NHL.</p><p><strong>Methods: </strong>209 NHL patients and 259 healthy subjects were studied. The salting out method was used for genomic DNA extraction, followed by the Refractory fragment length polymorphism polymerase chain reaction (RFLP-PCR) technique for genotyping. SPSS package V.22 software was used for statistical analysis. Several in silico tools were used to predict the probable consequences of studied H19 genetic variants on the different aspects of non-coding RNAs.</p><p><strong>Results: </strong>The results revealed that statistically, both rs3741219T>C and rs217727C>T variants increased the susceptibility to NHL. The T allele of rs3741219T>C in the codominant model caused the most enhancement in the incidence of NHL (OR = 2.33, 95% CI = 1.28-4.25, p = 0.005). Moreover, The CC genotype of rs217727C>T compared to TT had the sharpest impact on the susceptibility to NHL (OR = 2.27, 95% CI = 1.21-4.23, p = 0.009). In silico predictions revealed that the studied variants seem to alter the binding sites of miRNAs on the H19 long non-coding RNA and change its targets. Furthermore, nucleotide substitution in both rs3741219T>C and rs217727C>T may prepare a new binding site for a transcription factor called Y-Box-binding protein-1 (YB-1).</p><p><strong>Conclusions: </strong>The rs217727C>T and rs3714219T>C were responsible for elevating the likelihood of NHL in our population. These substitutions alter the RNA folding of H19 and alter the miRNA binding sites on the H19 transcript.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70024"},"PeriodicalIF":2.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}