Journal of Clinical Laboratory Analysis最新文献_第2页

Expression Profile of Serum CircFUNDC1 and CircUHRF1 Can Differentiate Between Colorectal Cancer and Inflammatory Bowel Diseases (Ulcerative Colitis and Crohn's Disease). 血清CircFUNDC1和cirhrf1的表达谱可以区分结直肠癌和炎症性肠病（溃疡性结肠炎和克罗恩病）

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-16 DOI: 10.1002/jcla.70039

Marwa A Ali, Olfat G Shaker, Eman M Ezzat, Abeer A Khalefa, Naglaa A Ahmed, Raghda Yahia Abu El-Ela, Amal A Ibrahim Amin, Fatma Abdel Hamid Mohamed, Marwa Mamdouh Ahmed Abdelhafeez, Mohamed Saad Zaghloul Ahmed, Marwa N AbdelHafez, Abdulrhman Khaled Al Abdulqader, Reham Fares

{"title":"Expression Profile of Serum CircFUNDC1 and CircUHRF1 Can Differentiate Between Colorectal Cancer and Inflammatory Bowel Diseases (Ulcerative Colitis and Crohn's Disease).","authors":"Marwa A Ali, Olfat G Shaker, Eman M Ezzat, Abeer A Khalefa, Naglaa A Ahmed, Raghda Yahia Abu El-Ela, Amal A Ibrahim Amin, Fatma Abdel Hamid Mohamed, Marwa Mamdouh Ahmed Abdelhafeez, Mohamed Saad Zaghloul Ahmed, Marwa N AbdelHafez, Abdulrhman Khaled Al Abdulqader, Reham Fares","doi":"10.1002/jcla.70039","DOIUrl":"https://doi.org/10.1002/jcla.70039","url":null,"abstract":"Background: Colorectal cancer (CRC) is a worldwide burden. Circular RNAs are promising biomarkers for diagnosing and prognosis of CRC.Objective: To investigate the possible association of sera levels of CircFUNDC1 and CircUHRF1 expression with predisposition and clinicopathological findings in CRC, ulcerative colitis (UC), and Crohn's disease (CD) in Egyptian patients.Methods: The serum levels of CircFUNDC1 and CircUHRF1 were evaluated in 113 Egyptian subjects divided into four groups; CRC (31), UC (26), and CD (25) and compared to healthy controls (31) using quantitative polymerase chain reaction.Results: The median values of log2 serum fold change (FC) of CircFUNDC1 in CRC, UC, and CD patients were 9.11, 6.58, and 6.17, respectively. It was upregulated in all case groups. CRC, UC, and CD patients had significantly higher serum CircFUNDC1 levels than controls (p < 0.001). However, there were no significant differences among patient groups (CRC, UC, and CD). The medians of log 2 of serum FC CircUHRF1 in patients with CRC, UC, and CD were -2.00, 3.33, and 3.12, respectively. The CircUHRF1 serum level was lower in the CRC group of patients, with no significant difference between the CRC group and the controls. Serum CircUHRF1 was significantly overexpressed in patients with UC and CD compared to the CRC groups or controls (p < 0.001). By Roc curve analysis, both genes can differentiate CRC patients from inflammatory bowel disease (IBD) patients or healthy controls with p < 0.05.Conclusion: Serum CircFUNDC1 is a biomarker for CRC, while CircUHRF1 is a biomarker of IBD.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70039"},"PeriodicalIF":2.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Preliminary Association Study of H19 Non-Coding Gene Variants With Risk of Non-Hodgkin Lymphoma: A Case-Control Study and Computational Analysis. H19非编码基因变异与非霍奇金淋巴瘤风险的初步关联研究：病例对照研究和计算分析

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-16 DOI: 10.1002/jcla.70024

Sara Kashani, Hoseinali Sasan, Behrouz Mollashahi, Gholamreza Bahari, Seyed Mahdi Hashemi, Mohsen Taheri

{"title":"A Preliminary Association Study of H19 Non-Coding Gene Variants With Risk of Non-Hodgkin Lymphoma: A Case-Control Study and Computational Analysis.","authors":"Sara Kashani, Hoseinali Sasan, Behrouz Mollashahi, Gholamreza Bahari, Seyed Mahdi Hashemi, Mohsen Taheri","doi":"10.1002/jcla.70024","DOIUrl":"https://doi.org/10.1002/jcla.70024","url":null,"abstract":"Background: Non-Hodgkin lymphoma (NHL) is one of the most prevalent disorders worldwide, with a variety range of etiology from environmental to genetic factors. H19 is a non-coding RNA that codes no protein while playing regulatory roles and is hypothesized to be involved in susceptibility to NHL.Methods: 209 NHL patients and 259 healthy subjects were studied. The salting out method was used for genomic DNA extraction, followed by the Refractory fragment length polymorphism polymerase chain reaction (RFLP-PCR) technique for genotyping. SPSS package V.22 software was used for statistical analysis. Several in silico tools were used to predict the probable consequences of studied H19 genetic variants on the different aspects of non-coding RNAs.Results: The results revealed that statistically, both rs3741219T>C and rs217727C>T variants increased the susceptibility to NHL. The T allele of rs3741219T>C in the codominant model caused the most enhancement in the incidence of NHL (OR = 2.33, 95% CI = 1.28-4.25, p = 0.005). Moreover, The CC genotype of rs217727C>T compared to TT had the sharpest impact on the susceptibility to NHL (OR = 2.27, 95% CI = 1.21-4.23, p = 0.009). In silico predictions revealed that the studied variants seem to alter the binding sites of miRNAs on the H19 long non-coding RNA and change its targets. Furthermore, nucleotide substitution in both rs3741219T>C and rs217727C>T may prepare a new binding site for a transcription factor called Y-Box-binding protein-1 (YB-1).Conclusions: The rs217727C>T and rs3714219T>C were responsible for elevating the likelihood of NHL in our population. These substitutions alter the RNA folding of H19 and alter the miRNA binding sites on the H19 transcript.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70024"},"PeriodicalIF":2.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the Role of Antimicrobial Peptides in the Management of Sexually Transmitted Infections. 抗菌肽在性传播感染治疗中的作用研究进展。

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-14 DOI: 10.1002/jcla.70041

Shuangwen Xiao, Wei Zuo, Qing Xiang, Yafeng Xie, Yongjian Xiao

{"title":"Advances in the Role of Antimicrobial Peptides in the Management of Sexually Transmitted Infections.","authors":"Shuangwen Xiao, Wei Zuo, Qing Xiang, Yafeng Xie, Yongjian Xiao","doi":"10.1002/jcla.70041","DOIUrl":"https://doi.org/10.1002/jcla.70041","url":null,"abstract":"Background: Sexually transmitted infections (STIs) are a major threat to global health, and the emergence of antibiotic-resistant strains has made therapeutic strategies more complex. Antimicrobial peptides (AMPs) are a ubiquitous class of natural compounds that are expected to be an alternative to conventional antibiotics due to their broad spectrum of activity and lower propensity for resistance, promising alternatives to conventional antibiotics.Objective: To emphasize the importance of antimicrobial peptides in the fight against STIs and to review recent advances in AMPs for the treatment and prevention of STIs.Methods: This article focuses on reviewing the progress of research on AMPs in the treatment and prognosis of STIs such as gonorrhea, HIV, HPV, and chlamydia, and discusses the challenges and future directions of the field.Results: AMPs have great potential in the prevention and treatment of STIs. However, AMPs for the treatment of STIs face challenges such as enzymatic degradation, safety and high cost, while nanotechnology and peptide modification are expected to enhance the stability and bioavailability of AMPs.Conclusion: AMPs have the potential to become an important tool for the treatment of STIs with further research and technological innovation.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70041"},"PeriodicalIF":2.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Differentially Expressed Proteins Involved in Shrimp and Crab Allergies. 虾蟹过敏相关差异表达蛋白分析。

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-14 DOI: 10.1002/jcla.70053

Ruhong Tan, Xiwen Wei, Jiacheng Zhong, Yuanbin Wang, Qing Yu

引用次数: 0

Fluctuations and Changes in Acute Phase Reactive Proteins in Fasting and Nonfasting States. 空腹和非空腹状态下急性期反应蛋白的波动和变化。

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-10 DOI: 10.1002/jcla.70052

Ben Huang, Shuxian Miao, Yan Xu, Si-Jie Qiu, Rui-Xia Yang, Hua-Guo Xu

{"title":"Fluctuations and Changes in Acute Phase Reactive Proteins in Fasting and Nonfasting States.","authors":"Ben Huang, Shuxian Miao, Yan Xu, Si-Jie Qiu, Rui-Xia Yang, Hua-Guo Xu","doi":"10.1002/jcla.70052","DOIUrl":"https://doi.org/10.1002/jcla.70052","url":null,"abstract":"Background: In clinical practice, acute-phase reactive proteins (APRPs) are frequently measured at random times. However, it is unclear whether the use of fasting or nonfasting samples affects results. This study aims to investigate the variations of APRPs between fasting and nonfasting conditions.Methods: This study was conducted based on the oral glucose tolerance test (OGTT) experiment due to standard energy intake and strict time flow. Fifty subjects were enrolled and underwent a 12-h fasting period before the experiment. Blood samples were collected the following day at baseline (fasting, T0) and 30 (T1), 60 (T2), 120 (T3), 180 (T4) minutes postglucose intake. A total of 250 blood samples were obtained. To quantify clinical fluctuations, percentage bias was calculated, and Bland-Altman plots were employed.Results: Our observational study demonstrated significant postprandial variations for APRPs. For CRP, 17 (34%) of 50 subjects at T1, 21 (42%) at T2, 23 (46%) at T3, and 16 (32%) at T4 exhibited levels exceeding the maximum allowable error in medical laboratory testing, indicating clinically unacceptable bias. For IL-6, thirty subjects (60%) at T1, 27 (54%) at T2, 28 (56%) at T3, and 32 (64%) at T4 displayed clinically unacceptable fluctuations. Among other APRPs, the maximum number of subjects exceeding acceptable bias thresholds was 28% (14/50) for procalcitonin, 38% (19/50) for transferrin, 34% (17/50) for prealbumin, and 24% (12/50) for ceruloplasmin.Conclusion: Clinical fluctuations were observed in the levels of APRPs between fasting and nonfasting states. Clinicians should pay attention to the effects of dietary factors on test results.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70052"},"PeriodicalIF":2.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of In-House Conventional and Multiplex PCR Methods for the Detection and Identification of Lophomonas spp.: An Innovative Approach. 开发和验证内部常规和多重PCR检测和鉴定Lophomonas spp.：一种创新的方法。

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-08 DOI: 10.1002/jcla.70049

Maryam Nakhaei, Mahdi Fakhar, Abouzar Bagheri, Hajar Ziaei Hezarjaribi, Saied Abediankenari, Ali Sharifpour, Maryam Ghasemi

{"title":"Development and Validation of In-House Conventional and Multiplex PCR Methods for the Detection and Identification of Lophomonas spp.: An Innovative Approach.","authors":"Maryam Nakhaei, Mahdi Fakhar, Abouzar Bagheri, Hajar Ziaei Hezarjaribi, Saied Abediankenari, Ali Sharifpour, Maryam Ghasemi","doi":"10.1002/jcla.70049","DOIUrl":"https://doi.org/10.1002/jcla.70049","url":null,"abstract":"Background: Pulmonary lophomoniasis is an emerging disease caused by the protozoan pathogen Lophomonas spp. Recently, a conventional polymerase chain reaction (PCR) method has been developed. However, its sensitivity and specificity remain to be fully established. Therefore, this study aimed to develop in-house conventional and multiplex PCR for the detection and identification of Lophomonas infections. Additionally, we attempted to compare the diagnostic performance of these novel PCR tests with the current microscopic examination method using BAL samples.Methods: We studied 120 bronchoalveolar lavage (BAL) specimens of the patients clinically suspected of having lophomoniasis. The specimens were examined using three methods: microscopic examination (Giemsa staining), in-house conventional PCR, and multiplex-PCR. Moreover, multiplex-PCR was used for the simultaneous identification of two species of Lophomonas.Results: Out of the 120 BAL specimens tested, 30 (25%) tested positive through microscopic wet mount examination. Among the three techniques, multiplex-PCR was the most sensitive (100%, 95% CI, 88.3-100), while Giemsa staining had the lowest sensitivity (86.2%, 95% CI, 69.4-94.5). The data reveal a strong agreement between multiplex-PCR and conventional PCR (κ = 0.96), while the lowest agreement was found between multiplex-PCR and microscopy methods (κ = 0.16). The study also confirmed the presence of L. blattarum species in all samples using multiplex-PCR.Conclusions: This study demonstrates that the in-house multiplex-PCR is a robust and accurate diagnostic test for the detection and identification of Lophomonas species. Therefore, our findings suggest that this method may be a powerful tool to overcome some diagnostic pitfalls for lophomoniasis.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70049"},"PeriodicalIF":2.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage and Preeclampsia: Macrophage Polarization Imbalance at the Maternal-Fetal Interface. 巨噬细胞与子痫前期：母胎界面巨噬细胞极化失衡。

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-08 DOI: 10.1002/jcla.70046

Yao Wei, Yang Su, Cheng Liu, Xiaolong Ma, Zihui Ling, Yilin Wang, Rui Qiao

{"title":"Macrophage and Preeclampsia: Macrophage Polarization Imbalance at the Maternal-Fetal Interface.","authors":"Yao Wei, Yang Su, Cheng Liu, Xiaolong Ma, Zihui Ling, Yilin Wang, Rui Qiao","doi":"10.1002/jcla.70046","DOIUrl":"https://doi.org/10.1002/jcla.70046","url":null,"abstract":"Background: Preeclampsia (PE), as a pathological pregnancy process, is still unclear in its precise pathophysiology. The current consensus on PE pathogenesis is that it is an immune-inflammatory response due to placental dysfunction leading to multiorgan involvement in the mother. Macrophages can polarize into different phenotypes under the influence of distinct microenvironments, secreting various cytokines or chemokines with distinct functions. These phenotypes play roles in either promoting inflammation or facilitating tissue repair. Studies have observed the increase in M1 polarization of decidual macrophages during the occurrence of PE, with this polarization imbalance contributing to the immuno-inflammatory response involved in placental formation. Therefore, understanding the polarization characteristics of macrophages provides a valuable direction for research related to the prevention and treatment of PE.Methods: Authors searched for related literature on PubMed using the professional terms \"preeclampsia\" and \"macrophage polarization\". The obtained literature was categorized according to its research. Similar articles are summarized in the same sections, which are divided into different small sections according to their specific contents.Results: Different studies have explored the metabolic characteristics, surface markers, secretions, signaling pathways, and functions of different macrophage polarization types, highlighting the critical role of polarization imbalance and excessive inflammatory responses in the development of PE. Intervening in inflammatory responses at the maternal-fetal interface holds significant value for the prevention and treatment of PE.Conclusion: Understanding the metabolic characteristics of different macrophage polarization types, combined with their polarization imbalance during the development of PE, can facilitate targeted prevention of PE.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70046"},"PeriodicalIF":2.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prenatal Diagnosis and Clinical Phenotypic Heterogeneity of 22q11.2 Microdeletion Syndrome Based on a Single Center Retrospective Study. 基于单中心回顾性研究的22q11.2微缺失综合征产前诊断及临床表型异质性

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-05 DOI: 10.1002/jcla.70045

Jia-Yan Chen, Mei-Jiao Cai, Yun-Sheng Ge

{"title":"Prenatal Diagnosis and Clinical Phenotypic Heterogeneity of 22q11.2 Microdeletion Syndrome Based on a Single Center Retrospective Study.","authors":"Jia-Yan Chen, Mei-Jiao Cai, Yun-Sheng Ge","doi":"10.1002/jcla.70045","DOIUrl":"https://doi.org/10.1002/jcla.70045","url":null,"abstract":"Objective: To retrospectively investigate the incidence of prenatal diagnosis of 22q11.2 microdeletion syndrome (22q11.2DS) in a single center and summarize its clinical manifestations to expand the phenotypic database.Methods: Pregnant women who underwent prenatal diagnosis at The Women and Children's Hospital, School of Medicine, Xiamen University, from January 2018 to February 2024 were retrospectively analyzed. Prenatal diagnosis was performed using routine G-banding karyotype analysis and chromosomal microarray analysis (CMA) or copy number variation sequencing (CNV-seq). Fetuses diagnosed with 22q11.2DS were further analyzed using detailed ultrasound diagnostic records to summarize the clinical manifestations of 22q11.2DS.Results: A total of 24,319 pregnant women underwent prenatal diagnosis, and 24 cases were diagnosed with 22q11.2DS, with an incidence of 0.99‰ (24/24319), including 16 cases of congenital heart disease, 4 cases of renal pelvis separation, 3 cases of cleft lip and palate, 2 cases of double strephenopodia, 2 cases of nasal bone dysplasia, and 1 case each of unclear thymus, spina bifida with meningomyelocele, abnormal fetal growth retardation, and NT thickening.Conclusion: Congenital heart disease was the most common phenotype in 22q11.2DS, and other malformations also occurred in a certain proportion. In addition, some rare clinical phenotypes, such as spina bifida with myelomeningocele and nasal bone hypoplasia, were also found in this cohort, which should be taken seriously to improve the detection rate of fetal 22q11.2DS.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70045"},"PeriodicalIF":2.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Biomarkers for Improving the Diagnosis of Appendicitis in Pediatric Patients. 改善小儿阑尾炎诊断的新生物标志物。

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-03 DOI: 10.1002/jcla.70048

Adir Alper, Yizhak Aronov, Lior Shlomov, Osnat Zmora

{"title":"Novel Biomarkers for Improving the Diagnosis of Appendicitis in Pediatric Patients.","authors":"Adir Alper, Yizhak Aronov, Lior Shlomov, Osnat Zmora","doi":"10.1002/jcla.70048","DOIUrl":"https://doi.org/10.1002/jcla.70048","url":null,"abstract":"Introduction: Diagnosing appendicitis in pediatric patients remains a clinical challenge, especially in resource-limited settings where imaging tools are less accessible. Inflammatory markers, including the Neutrophil-to-Lymphocyte Ratio (NLR), Monocyte-to-Lymphocyte Ratio (MLR), Neutrophil-to-Monocyte Ratio (NMR), Platelet-to-Lymphocyte Ratio (PLR), Neutrophil-to-Platelet Ratio (NPR), and C-reactive protein (CRP), offer a promising approach to enhancing diagnostic accuracy. We aimed to evaluate the utility of these inflammatory markers to diagnose appendicitis.Methods: This retrospective study included 1027 pediatric patients who underwent appendectomy, with appendicitis confirmed histopathologically in 891 cases. Preoperative Inflammatory markers (NLR, MLR, NMR, PLR, NPR, and CRP) were analyzed and optimal cutoff values were determined using Receiver Operating Characteristic (ROC) curves.Results: Elevated NLR, NMR, PLR, NPR, and CRP were strongly associated with appendicitis, while an inverse relationship was observed with MLR. NLR (≥ 4.42) and NPR (≥ 0.0327) demonstrated high diagnostic accuracy with sensitivity and specificity exceeding 75%. Surprisingly, MLR showed a statistically significant inverse relationship with AA risk. Temperature differences between groups were not statistically significant.Conclusion: Each suggested novel inflammatory marker has the potential to improve the preoperative diagnosis of appendicitis in pediatric patients. Such a system could minimize reliance on imaging and expedite decision-making, especially in resource-constrained settings. Further prospective studies are needed to validate these findings and explore their clinical utility.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70048"},"PeriodicalIF":2.6,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endurance Training Alleviates Metabolic-Associated Fatty-Liver Disease (MAFLD)-Related Testicular Impairments via Endoplasmic Reticulum Stress Regulation. 耐力训练通过内质网应激调节减轻代谢相关脂肪性肝病（MAFLD）相关睾丸损伤。

IF 2.6 4区医学

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-01 DOI: 10.1002/jcla.70042

Nastaran Teimouri, Vahid Kazemizadeh

{"title":"Endurance Training Alleviates Metabolic-Associated Fatty-Liver Disease (MAFLD)-Related Testicular Impairments via Endoplasmic Reticulum Stress Regulation.","authors":"Nastaran Teimouri, Vahid Kazemizadeh","doi":"10.1002/jcla.70042","DOIUrl":"https://doi.org/10.1002/jcla.70042","url":null,"abstract":"Background: Metabolic-associated fatty liver disease (MAFLD), the most prevalent liver disorder globally, affects 20%-40% of the population and presents significant health challenges. Studies link MAFLD to male reproductive dysfunction, highlighting the need for effective interventions. This study investigates the impact of MAFLD on testicular function and evaluates the protective role of endurance training, with a focus on the GRP78-IRE-1α-ATF6 signaling pathway.Methods: Forty-four rats were allocated into two dietary groups (n = 22 each): a standard diet control group (C) and a high-fat diet supplemented with fructose water group (FL). After 17 weeks, histological analysis confirmed MAFLD development in the FL group, while the control group showed no pathological changes. Each dietary group was further subdivided into sedentary and endurance-trained (T) subgroups (n = 10 per subgroup), resulting in four experimental groups: C, C + T, FL + T, and FL. At the end of the research, thyroid stimulating hormone (TSH), sex hormones (testosterone), tumor necrosis factor-alpha (TNF-a) as well as GRP78, IRE-1α, and AFT6 expression were assessed.Results: Our results indicated that MAFLD led to significant weight gain, disrupted serum levels of thyroid-stimulating hormone, and impaired sex hormone profile. Additionally, MAFLD triggered ER stress, evidenced by dysregulated expression of genes in the GRP78-IRE-1α-ATF6 pathway. Remarkably, endurance training mitigated these adverse effects by normalizing hormonal profiles and restoring the expression of ER stress-related genes. These findings highlight the critical role of ER stress in MAFLD-induced male reproductive dysfunction.Conclusion: Overall, the present study suggests endurance training as a promising treatment strategy for addressing MAFLD and its associated reproductive complications.","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70042"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0