Journal of Clinical Laboratory Analysis最新文献

{"title":"Antifungal Resistance Among Candida Species: Diagnostic and Clinical Challenges in Specialized Cancer Care Hospital of Lahore.","authors":"Zunaira Akbar, Muhammad Aamir, Zikria Saleem, Muhammad Rehan Khan Niazi, Hasan Ejaz, Muharib Alruwaili, Khalid Abosalif","doi":"10.1002/jcla.70022","DOIUrl":"https://doi.org/10.1002/jcla.70022","url":null,"abstract":"<p><strong>Background: </strong>The increased consumption of antifungal agents increases the emergence of resistant fungal strains among immunocompromised patients. The present study aims to determine the antifungal consumption and resistance pattern among adult and pediatric cancer patients with systemic Candida infections.</p><p><strong>Method: </strong>A descriptive, retrospective study was conducted by reviewing admitted patients' electronic medical records from 2019 to 2023. Consumption was estimated using Days of Therapy (DOT) metrics. Culture reports of Candida-positive isolates were collected from the hospital microbiology laboratory to determine the resistance pattern of Candida species.</p><p><strong>Results: </strong>Consumption of antifungals showed a declining trend, with highest consumption of oral voriconazole (mean DOT/1000 pt. days 18653.49) and oral fluconazole (mean DOT/1000 pt. days 15258.14). Candida albicans was the most isolated pathogen 768 (53.60%) and among all Candida species, major resistance was found in C. tropicalis 58 (4.05%). All Candida species except C. parapsilosis and C. guilliermondii exhibited more resistance to fluconazole.</p><p><strong>Conclusion: </strong>The findings indicated that the number of resistant isolates is clinically important 133 (9.2%) in the immunocompromised population, which underscores the necessity of conducting culture sensitivity analyses of antifungals. This approach is crucial for early detection and mitigation of antifungal resistance and optimizing therapy.</p>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":" ","pages":"e70022"},"PeriodicalIF":2.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Progression of Vitamin D Deficiency in Greater Beirut and Mount Lebanon From 2013 to 2022: An Analysis of 19,452 Adults","authors":"Pia Chedid,&nbsp;Elie Salem-Sokhn,&nbsp;Said El Shamieh,&nbsp;Rajaa Fakhoury","doi":"10.1002/jcla.70023","DOIUrl":"10.1002/jcla.70023","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This study aimed to describe the prevalence and progression of vitamin D (VitD) deficiency in a large sample of Lebanese adults.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A retrospective analysis of 19,452 medical records of Lebanese adults primarily residing in Greater Beirut and Mount Lebanon was included. Serum VitD levels were measured using a chemiluminescent assay.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Overall, 31% of our participants were deficient (&lt; 20 ng/mL), 28% were insufficient (20–30 ng/mL), and 41% were sufficient (&gt; 30 ng/mL) for VitD. The overall average VitD levels were 26 ± 12 ng/mL (min: 3 ng/mL, max: 220 ng/mL). While 40% of participants below 35 years old presented with VitD level deficiency, this level decreased significantly by 13% and 11% in age groups 55–65 and above 65 years old, respectively (<i>p</i> &lt; 0.01). Females had a 35% lower risk of VitD deficiency than males (OR = 0.65, <i>p</i> &lt; 0.01). Second, living in the greater Beirut region increased the risk of developing VitD deficiency by 41% (OR = 1.408, <i>p</i> &lt; 0.01). Finally, living in a region with moderate (OR = 1.33, <i>p</i> &lt; 0.11) to high pollution (OR = 1.45, <i>p</i> &lt; 0.01) increased the risk by 33% and 45%, respectively. The number of individuals referred for testing VitD level in our tertiary healthcare center increased four times in the past 10 years, from 1120 in 2013 to 4633 in 2022. This observation is correlated with higher VitD levels and, thus, a significant decrease in the trend of VitD deficiency in 2022 compared to the previous years (<i>p</i> &lt; 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The prevalence of VitD deficiency has decreased over the past 10 years in Greater Beirut and Mount Lebanon.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk Factors and Clinical Value of Deep Venous Thrombosis After Traumatic Lower Limb Fractures","authors":"XiuYun Zeng,&nbsp;Dan Zhu,&nbsp;ZhiFeng Xiao,&nbsp;HongLian Lin","doi":"10.1002/jcla.70019","DOIUrl":"10.1002/jcla.70019","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Deep vein thrombosis (DVT) is a common complication after traumatic lower limb fractures. This study aims to investigate the risk factors associated with DVT after traumatic lower limb fractures and to analyze their values in clinical practice.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Patients were classified into DVT and Non-DVT groups based on the occurrence of DVT during the perioperative period. These data were statistically analyzed to screen for risk factors for DVT after traumatic lower limb fractures and to analyze thrombus regression 1 month after surgery.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>DVT occurred in 39.29% (77/196) patients during the perioperative period. The incidence of DVT varied, with a high incidence of 46.67% for femoral neck/trochanteric fractures. Patients in the DVT group were characterized by older age, higher perioperative blood transfusion (both <i>p</i> &lt; 0.001), higher fibrinogen (FIB) on admission (<i>p</i> &lt; 0.001), significant postoperative Hb decline (<i>p</i> = 0.023) and higher D-D (<i>p</i> = 0.001). Age greater than 65 years, comorbid diabetes mellitus, and postoperative Hb decline of 30 g/L were independent risk factors for DVT in the perioperative period in patients with traumatic lower limb fractures. For every 1 SD increase in FIB and D-D, patients had a 1.01-fold (95% CI, 0.99 to 4.25) and 2.75-fold (95% CI, 1.055 to 7.25) increased risk of DVT, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>DVT complicating traumatic lower limb fractures is strongly associated with a variety of risk factors, of which advanced age and significantly higher D-D on admission are two of the most critical.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Effect of FT3 Within the Euthyroid Range on HDL-C in Patients With Type 2 Diabetes: A Cross-Sectional Analysis of Inpatients in China","authors":"Jinmei Xu,&nbsp;Shangshuang Zhao,&nbsp;Yan Wang,&nbsp;Jun Han","doi":"10.1002/jcla.70029","DOIUrl":"10.1002/jcla.70029","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Evidence for assessing the relationship between free triiodothyronine (FT3) and high-density lipoprotein cholesterol (HDL-C) remains limited. Therefore, the purpose of our study is to evaluate the relationship between FT3 and HDL-C in patients with type 2 diabetes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>From June 2022 to October 2023, 3011 patients with normal thyroid function and diagnosed with type 2 diabetes mellitus (T2DM) were collected continuously and non-selectively in a Chinese hospital. Then, we used a logistic regression model to explore the relationship between FT3 and HDL-C. Smooth curve fitting is used to identify the nonlinear relationship between FT3 and HDL-C.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After adjusting for the influence of relevant factors, FT3 and HDL-C were negatively correlated −0.02 (−0.04, −0.00; <i>p</i> = 0.0162). There is also a nonlinear relationship between FT3 and HDL-C, with an inflection point of 3.48 pmol/L for FT3 (P for log- likelihood ratio test = 0.044).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study shows that there is a negative correlation and nonlinear relationship between FT3 and HDL-C in the Chinese population with diabetes. When FT3 is between 2.76–3.48 pmol/L, HDL-C tends to a stable state; When FT3 is between 3.48–6.45 pmol/L, HDL-C decreases with the increase of FT3 concentration (According to the reference range used by our hospital, the normal value of serum FT3 is 2.76–6.45 pmol/L). These findings suggest that maintaining FT3 within the range of 2.76 to 3.48 pmol/L may be most beneficial for mitigating the progression of cardiovascular disease in patients with T2DM.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining the Usefulness of Selected Laboratory Markers of Inflammation in Qualifying Patients for T2 Biosystems Determination","authors":"Mateusz Szymański,&nbsp;Małgorzata M. Skiba,&nbsp;Małgorzata Piasecka","doi":"10.1002/jcla.70021","DOIUrl":"10.1002/jcla.70021","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Improving treatment outcomes sepsis requires early recognition, the implementation of appropriate treatment, and targeted antimicrobial therapy. Nowadays, microbiological diagnostic methods are available to accelerate microbiological diagnosis, thereby reducing the time needed to implement targeted antibiotic therapy. One method for rapid diagnosis is the amplified magnetic resonance imaging—T2 Biosystems, USA (T2Dx). This method enables the identification of pathogens directly from a blood sample (approximately 4 mL) within about 3.5 h. The use of the “T2 Resistance” panel additionally allows for the detection of the most common bacterial resistance mechanisms in about 4–5 h. The disadvantage of the T2Dx method is the limited number of microorganisms it can detect. The objective of the study was to evaluate the effectiveness of using selected inflammatory parameters to accurately qualify patients (positive result) for T2Dx testing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We have made a retrospective evaluation of selected inflammatory parameters in order to determine which parameters are the best indicators for good qualification of patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results and Conclusion</h3>\u0000 \u0000 <p>A single analysis of parameters such as C-reactive protein (CRP), white blood cells (WBC), #neutr, #lymph, and neutrophil-to-lymphocyte ratio (NLR) is not a good indicator that could be used as an additional tool facilitating patient qualification for T2Dx testing. The most sensitive parameter distinguishing between patients with a positive T2Dx result and those with a negative result is the measurement of IL-6 and PCT. Proper patient qualification for T2Dx testing can significantly contribute to reducing the time to initiate targeted antibiotic therapy and may impact reducing mortality and improving long-term treatment outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Occupational Health and Safety Levels in Genetic Disease Centers in Istanbul","authors":"Vedat Caner,&nbsp;Ferdi Tanir","doi":"10.1002/jcla.70015","DOIUrl":"10.1002/jcla.70015","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Genetic disorders significantly impact public health and quality of life, necessitating precise and timely diagnosis for effective risk management and treatment. Genetic diagnostic centers (GDCs) play a critical role in this process but face numerous occupational health and safety (OHS) challenges. The classification of GDCs based solely on biosafety levels is insufficient for assessing their overall OHS conditions. This study aims to systematically evaluate OHS practices in GDCs and propose a new classification approach based on hazard dimensions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This cross-sectional study was conducted in 15 GDCs in Istanbul, including two public and 13 private facilities with 75 employees. Data were collected through a structured survey with 49 statements covering seven hazard dimensions. Regression and correlation analyses were used to assess the impacts and interrelationships of these dimensions on risk management. Principal Component Analysis (PCA) was applied for dimensionality reduction, and the k-Nearest Neighbours (k-NN) algorithm classified laboratories into safety levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Personal protective equipment had the highest impact on risk management (56.3%), while physical security had the lowest (34.8%). Among the 21 identified hazard relationships, 18 were very strong and three were strong. PCA reduced the data into three primary components, explaining 81.9% of the variance. The k-NN algorithm achieved a classification accuracy of 93.33%, consolidating six hazard dimensions into three and categorizing centers into three safety levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The findings emphasize the need for an updated OHS classification for GDCs beyond biosafety levels. Integrating hazard dimensions into safety assessments can improve risk management and enhance laboratory safety standards.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 7","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Verification Process of a POC Blood Gas Analyser—The Nova Stat Profile Primer Plus Analyser","authors":"Joseph Costa,&nbsp;Ritienne Attard,&nbsp;the GGH Medical Laboratory Services team","doi":"10.1002/jcla.70006","DOIUrl":"10.1002/jcla.70006","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Point-of-care testing of blood gases plays a critical role in patient management. The aim of this study was to verify the manufacturer's specifications of the Nova Stat Profile Prime Plus Analyser, along with a comparison study with the GEM Premier 4000 Blood Gas Analyser.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Parameters analysed were pH, pCO₂, pO₂, Na⁺, Cl⁻, K⁺, iCa, lactate, and glucose. Data for the precision and bias study were generated using control samples in a 5 × 5 study design. Linearity was checked using a five-level Linearity Control Set, while comparison was done between the Nova and GEM analysers using whole blood samples (<i>N</i> = 103). Acceptance was based on the CLIA TE_a for all analytes except for lactate, for which the TE_a defined by CAP and AAB was used.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The within-run and between-run CV_R% precision were all lower than the claimed CVs%, except for pCO₂ control level 2 within run (CV% 1.5 [claim CV% 1.1]) and iCa control level 5 between run (CV% 1.42 [claim 1.12]). The observed bias for all parameters was within the calculated lower and upper bias limits. Linearity was verified for all analytes except for Na⁺. Upon comparison of the Nova and GEM analysers, a correlation coefficient above 0.95 was observed for most parameters.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The Nova Stat Profile Prime Plus analyser meets the manufacturer's precision and bias claims. Linearity was confirmed for most analytes. There was a good correlation between the Nova and GEM Blood Gas analyser at concentrations within the reference range intervals for all investigated parameters.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 7","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Immunomagnetic Bead Separation-Immunosensor Detection and Nested-PCR Methods for Detecting Mycobacterium avium Subspecies paratuberculosis in Cattle Feces","authors":"Mohammad Khosravi,&nbsp;Mohammad Nouri,&nbsp;Mohammad Rahim Haji Hajikolaei,&nbsp;Ali Kolivand,&nbsp;Darioush Gharibi,&nbsp;Peter. D Constable","doi":"10.1002/jcla.70009","DOIUrl":"10.1002/jcla.70009","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Johne's disease, also known as paratuberculosis, is a chronic granulomatous enteritis disease that affects ruminants worldwide.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The objective of this study was to assess the effectiveness of the immunomagnetic bead separation-immunosensor (IMB-IS) detection method compared to Nested-PCR for identifying <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) infection in cattle feces samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Ninety rectal fecal samples were collected from selected cattle, comprising 59 serum-positive and 31 serum-negative cases based on serum ELISA. Following DNA extraction, nested-PCR was conducted using the IS900 primer sequence targeting the MAP-specific gene. Immunomagnetic bead (IMB) nanoparticles were synthesized by purifying hyperimmune donkey IgG through affinity chromatography and then conjugating it to Fe nanoparticles. Rhodamine-B hydrazone immunosensor (IS) was synthesized and conjugated to hyperimmune rabbit IgG. The synthesized IMB and IS were used to identify MAP in cattle fecal samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The results of this study revealed that of the 90 stool samples tested using the nested-PCR method, 62 samples (68.88%) were positive, while 28 samples (31.12%) were negative. In the IMB-IS test based on optical density (OD), 64 samples were positive (71.1%), while 26 samples were negative (28.8%). This test exhibited a sensitivity of 100%, specificity of 92.85%, and an overall test accuracy of 97.77%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Given the considerations of cost, time, positive and negative predictive values, and acceptable accuracy of the IMB-IS test, it is recommended for evaluation in screening and epidemiological studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 7","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Nanobiosensors for Rapid and Sensitive Detection of Dengue Virus Biomarkers by Using Clinical Laboratory","authors":"Seyedeh Niloufar Rajaei,&nbsp;Mohammad Darvish,&nbsp;Alireza Zare,&nbsp;Hamed Abdollahi,&nbsp;Vahid Alivirdiloo,&nbsp;Mobasher Hajiabbasi,&nbsp;Farhood Ghazi,&nbsp;Ahmad Mobed","doi":"10.1002/jcla.70012","DOIUrl":"10.1002/jcla.70012","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The recent rise in dengue virus (DENV) cases poses a significant threat to human health, with infections ranging from mild to severe and potentially leading to premature death.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To highlight the importance of early detection of DENV and to review advancements in detection technologies, particularly focusing on nanobiosensors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This review examines traditional detection methods for DENV, including molecular, serological, and direct virus culture techniques, while discussing their limitations. It also explores innovative technologies that enhance detection accuracy, speed, and efficiency.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Nonstructural protein 1 (NS1) serves as a key biomarker present in high concentrations during the early stages of DENV infection, underscoring the need for timely detection. Traditional methods, while effective, have limitations that new technologies aim to address. Biosensors, particularly nanobiosensors, have emerged as promising tools for rapid, sensitive, and cost-effective DENV detection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The adoption of advanced detection methods, especially nanobiosensors, is crucial for improving DENV management and reducing human suffering. This review provides a comprehensive overview of nanobiosensors and their applications, presented in an accessible manner for readers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 7","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Analysis of WES and scRNA-Seq Data Reveals the Genetic Basis of Immune Dysregulation in Unexplained Recurrent Pregnancy Loss","authors":"Zhao-Jing Lin,&nbsp;Lei Zhu,&nbsp;Yi Dong,&nbsp;Jiao Yun,&nbsp;Ya-Nan Zhi,&nbsp;Wei Zhang,&nbsp;Yan-Mei Sun,&nbsp;Yu-Jie Jiang,&nbsp;Shu Liu,&nbsp;Liang-Liang Fan,&nbsp;Ya-Li Li,&nbsp;Shuai Guo","doi":"10.1002/jcla.70011","DOIUrl":"10.1002/jcla.70011","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to identify genetic variants and their functional consequences underlying Unexplained Recurrent Pregnancy Loss (uRPL) through comprehensive genomic and transcriptomic analyses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We recruited 13 Chinese uRPL patients and performed Whole Exome Sequencing (WES) on chorionic villi samples from miscarriage tissues. Additionally, we conducted an integrative analysis using single-cell RNA sequencing data from decidual immune cells to examine expression patterns.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>WES analysis pinpointed variants in the four <i>MUC</i> genes (<i>MUC4</i>, <i>MUC6</i>, <i>MUC16</i>, and <i>MUC17</i>), six lipid metabolism genes in immune cells (<i>ABCA4</i>, <i>ABCA7</i>, <i>ABCB5</i>, <i>ABCC8</i>, <i>ADGRV1</i>, and <i>ANK3</i>), and two structural genes (<i>PIEZO1</i> and <i>PKD1</i>), whose variants impair mucosal barriers and lipid homeostasis, thereby leading to immune dysregulation and contributing to uRPL. To delve deeper into the effects of these genetic variants on cellular expression patterns, we undertook an integrative analysis using a single-cell dataset from decidual immune cells in uRPL cases. We observed significant dysregulation of lipid metabolism within immune cells, reduced heat shock protein expression, and enhanced chemokine signaling in uRPL samples, indicating a pro-inflammatory state.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In summary, our study reveals a complex interplay between genetic variants and immune cell dysfunctions in uRPL, emphasizing the role of identified genetic variants in driving pro-inflammatory states. These findings provide a comprehensive view of the molecular mechanisms underlying uRPL, opening paths for novel therapeutic interventions and improved clinical management.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}