Fluctuations and Changes in Acute Phase Reactive Proteins in Fasting and Nonfasting States

IF 2.9 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-05-10 DOI:10.1002/jcla.70052

Ben Huang, Shuxian Miao, Yan Xu, Si-Jie Qiu, Rui-Xia Yang, Hua-Guo Xu

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引用次数: 0

Abstract

Background

In clinical practice, acute-phase reactive proteins (APRPs) are frequently measured at random times. However, it is unclear whether the use of fasting or nonfasting samples affects results. This study aims to investigate the variations of APRPs between fasting and nonfasting conditions.

Methods

This study was conducted based on the oral glucose tolerance test (OGTT) experiment due to standard energy intake and strict time flow. Fifty subjects were enrolled and underwent a 12-h fasting period before the experiment. Blood samples were collected the following day at baseline (fasting, T0) and 30 (T1), 60 (T2), 120 (T3), 180 (T4) minutes postglucose intake. A total of 250 blood samples were obtained. To quantify clinical fluctuations, percentage bias was calculated, and Bland–Altman plots were employed.

Results

Our observational study demonstrated significant postprandial variations for APRPs. For CRP, 17 (34%) of 50 subjects at T1, 21 (42%) at T2, 23 (46%) at T3, and 16 (32%) at T4 exhibited levels exceeding the maximum allowable error in medical laboratory testing, indicating clinically unacceptable bias. For IL-6, thirty subjects (60%) at T1, 27 (54%) at T2, 28 (56%) at T3, and 32 (64%) at T4 displayed clinically unacceptable fluctuations. Among other APRPs, the maximum number of subjects exceeding acceptable bias thresholds was 28% (14/50) for procalcitonin, 38% (19/50) for transferrin, 34% (17/50) for prealbumin, and 24% (12/50) for ceruloplasmin.

Conclusion

Clinical fluctuations were observed in the levels of APRPs between fasting and nonfasting states. Clinicians should pay attention to the effects of dietary factors on test results.

Abstract Image

查看原文本刊更多论文

空腹和非空腹状态下急性期反应蛋白的波动和变化。

背景：在临床实践中，急性期反应蛋白（aprp）经常在随机时间测量。然而，目前尚不清楚使用禁食或非禁食样本是否会影响结果。本研究旨在探讨aprp在禁食和非禁食条件下的变化。方法：采用能量摄入标准、时间流程严格的口服葡萄糖耐量试验（OGTT）实验。50名受试者在实验前禁食12小时。次日在基线（禁食，T0）和葡萄糖摄入后30 （T1）、60 （T2）、120 （T3）、180 （T4）分钟采集血样。共采集了250份血样。为了量化临床波动，计算了百分比偏差，并采用Bland-Altman图。结果：我们的观察性研究显示了aprp在餐后的显著变化。在50名受试者中，T1组有17人（34%），T2组有21人（42%），T3组有23人（46%），T4组有16人（32%）的CRP水平超过医学实验室检测的最大允许误差，表明临床不可接受的偏倚。对于IL-6, 30名受试者（60%）在T1、27名（54%）在T2、28名（56%）在T3和32名（64%）在T4表现出临床不可接受的波动。在其他aprp中，超过可接受偏倚阈值的受试者最大人数降钙素原为28%(14/50)，转铁蛋白为38%(19/50)，白蛋白前为34%(17/50)，铜蓝蛋白为24%（12/50）。结论：空腹和非空腹状态aprp水平存在临床波动。临床医生应注意饮食因素对检测结果的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.