Journal of Biologically Active Products from Nature最新文献

{"title":"Evaluation of the Carotenoids Profile from Annona muricata and Protective Effects Against Copper Accumulation: Wilson’s Disease","authors":"Thaginne Garjioni, P. N. Pinheiro, Patrícia Acosta, Y. S. Neves, Katianne Wolf, L. Q. Zepka, E. Jacob‐Lopes, R. Moresco, A. Ourique, F. S. Bruckmann, C. R. Rhoden, D. Zanchet, A. R. Viana","doi":"10.1080/22311866.2022.2150311","DOIUrl":"https://doi.org/10.1080/22311866.2022.2150311","url":null,"abstract":"Abstract Wilson’s disease is characterized by the accumulation of copper (Cu) in the body and does not have a curative treatment, having a high rate of patients who abandon the use of treatment drugs. Thus, there is a need to use raw materials from natural and easily accessible sources, which mitigates the consequences of the disease and provides beneficial adjuvant effects. Medicinal plants are part of this context, Annona muricata, better known as soursop, has a widespread use all around the world. In the present study, the carotenoid profile from leaves of the plant was assessed by high performance liquid chromatography coupled with a photodiode array and mass spectrometry detectors. In parallel the bioactivity of carotenoid extract was evaluated. The cytotoxicity of the aerial parts of the plant was evaluated through the MTT assay, as well as the IC50 of the treatments with copper in cell lines 293T, BV-2, and RAW 264.7. The anti-inflammatory activity and production of nitric oxide induced by lipopolysaccharide (LPS) were also evaluated. A total of nine carotenoids were separated in Annona muricata, with lutein being the major one 72.87%. A. muricata showed important antioxidant, cytoprotective, and anti-inflammatory activity in in vitro model of Wilson’s disease. Finally, it can be concluded that A. muricata proves to be a potential candidate for future studies for therapeutic alternatives of chronic diseases. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"392 - 405"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49489929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory Activity Guided Fractionation of Aerial Parts of Clematis gouriana Roxb. ex DC","authors":"Ritu Kalia, S. Jachak","doi":"10.1080/22311866.2022.2124193","DOIUrl":"https://doi.org/10.1080/22311866.2022.2124193","url":null,"abstract":"Abstract Clematis gouriana Roxb. ex DC. also known as Indian Traveler’s Joy is used in different regions of India for curing skin infections, cuts and wounds. The study was performed on 70% methanol extract of C. gouriana aerial parts and its n-hexane, ethyl acetate and n-butanol fractions. The in vitro and in vivo anti-inflammatory potential was evaluated using inhibition of NO production in LPS-stimulated RAW 264.7 macrophages cell line and carrageenan-induced rat paw edema model in vivo, respectively. All the extracts and fractions exhibited concentration-dependent inhibitory effects. It was found that ethyl acetate fraction was the most potent fraction showing 77.24±0.71% inhibition of NO release at 100 μg/mL concentration. The ethyl acetate fraction of C. gouriana also showed significant anti-inflammatory activity in the carrageenan-induced rat paw edema model at a dose of 400 mg/Kg. Further, the phytochemical investigation of the bioactive ethyl acetate fraction led to isolation of five compounds belonging to phenolics and flavonoids type of natural products. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"378 - 391"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48105990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terpinen-4-ol, An Active Constituent of Kewda Essential Oil, Mitigates Biofilm Forming Ability of Multidrug Resistant Staphylococcus aureus and Klebsiella pneumoniae","authors":"Priya Cheruvanachari, Subhaswaraj Pattnaik, M. Mishra, P. Pragyandipta, P. Naik","doi":"10.1080/22311866.2022.2154264","DOIUrl":"https://doi.org/10.1080/22311866.2022.2154264","url":null,"abstract":"Abstract The increased incidence of microbial resistance to traditional antibiotics has urged the scientific community to look for alternative therapeutic regimens. In this context, mitigation of biofilm formation is considered as viable alternative. Since, plant derived essential oils are rich heritage of bioactive phytochemicals with widespread pharmacological values, in the present study Terpinen-4-ol, bioactive constituent of Kewda essential oil (KEO) extracted from Pandanus odorifer male flower was evaluated for its antimicrobial and antibiofilm activities against Staphylococcus aureus and Klebsiella pneumoniae and their reference strains, MTCC-740 and MTCC-109. The minimum inhibitory concentration (MIC) of Terpinen-4-ol against S. aureus and K. pneumoniae was 50 and 25 mM, respectively. At MIC level, Terpinen-4-ol exhibited antibacterial activities against both the reference strains i.e. MTCC-740 and MTCC-109 with a zone of 16 and 14 mm, respectively. On treatment with sub-MIC of Terpinen-4-ol, a significant reduction in exopolysaccharides (EPS) production was observed as evident from qualitative Congo red agar (CRA) assay. Further, the reduction in EPS production was quantified with a reduction of 67.51±1.29% against S. aureus. The light and fluorescence microscopic analysis also corroborated the antibiofilm potential of Terpinen-4-ol as a significant reduction in the thickness of biofilm formation was observed. In silico studies provided an insight into the action of Terpinen-4-ol in binding to target proteins associated with biofilm formation and drug resistance. Thus, Terpinen-4-ol could be considered as putative drug candidate in the fight against biofilm associated chronic infections and drug resistance. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"406 - 420"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46840736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivities of Phenylpropanoids Extracted from Ephemeral Flowers of Medicinal Woody Species Murraya paniculata (Linn.) Jack","authors":"Ishita Paul, Sandipta Ghosh, K. Acharya, P. Bhadoria","doi":"10.1080/22311866.2022.2125903","DOIUrl":"https://doi.org/10.1080/22311866.2022.2125903","url":null,"abstract":"Abstract Flowers of Murraya paniculata were assessed for production of phenolic secondary metabolites and were found to yield temporally variable concentrations of total phenolics and flavonoids due to enzymatic regulation by L-phenylalanine ammonia lyase. Maximum yield of phenolics was obtained from partly open flowers, with esterified hydroxycinnamic acids (mainly ferulic acid) forming the major component. Scopoletin-a coumarin product of ferulic acid - was isolated consistently from senescent flowers by High Performance Liquid Chromatography (HPLC). Approximately 200 mg of fresh floral tissue extracted with one millilitre solvent (methanol:water :: 30:70) yielded up to 1.6 mg of ferulic acid and 40 μg of scopoletin from partly open (at 18:00 h) and senescent (at 06:00 h) blooms respectively. Along with other hydroxycinnamic acid and coumarin components of M. paniculata flowers detected by HPLC, o-coumaric acid and isoauraptene were identified by Gas Chromatography-Mass Spectrometry. Various free-radical scavenging assays revealed the ferulic acid fraction to have stronger antioxidant activities than the scopoletin fraction, but no antibiotic activity was detected for ferulic acid. Total antioxidant capacities of ferulic acid and scopoletin fractions were estimated to be 9% and 7.8% of that of ascorbic acid standard, respectively. Scopoletin fraction exhibited Minimum Inhibitory Concentrations at 0.585 mg.mL-1 against Bacillus subtilis and 1.372 mg.mL-1 against Escherichia coli. The high concentrations of these bioactive phenylpropanoids in such a common flower species presents potential application in low-risk crop protection and plant-based nutraceuticals.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"353 - 365"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43791258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethno Pharmacological Perspectives of Peroxisome Proliferators Activated Receptors (PPARs) Isoform in the Treatment of Obesity: A Comprehensive Review","authors":"Anjali Kumari Atal, M. Choudhary, V. Budhwar","doi":"10.1080/22311866.2022.2025901","DOIUrl":"https://doi.org/10.1080/22311866.2022.2025901","url":null,"abstract":"Abstract Obesity is the most prevalent nutritional disease that naturally leads human beings to metabolic syndrome resulting in improper accumulation of ectopic lipids. The predominant source of obesity is the consumption of a high-energy diet and this excess energy is stored in adipocytes and leads to an increase in mass and volume. This study attempted to include an overview of the findings on traditional botanical sources for obesity treatment and their correlation with PPARs isoforms. The present study was reviewed by collecting data from standard textbooks, various reviews and research papers and consulting globally accepted online databases i.e. Scopus, Google Scholar, Science Direct, and Pub Med from 2000 to 2021. The profile presented includes the information on the biological source, their parts, extracts, active constituents, and experimental method and toxicity studies of plants via PPARs isoforms individually. 78 plant species, belonging to 50 families used for obesity are compiled. Major families screened in our study were belonging to Leguminosae, Myrtaceae, Polygonaceae, Compositae, Malvaceae, Vitaceae, Sapindaceae, and Araliaceae along with some others in a lesser amount. The study could be beneficial for preliminary screening of potential natural PPARs metabolites in obesity treatment and high time to increase experimental studies to find out novel potential active compounds/extracts from unexploited plants having a traditional role in obesity regulation. Further toxicity profiles might be beneficial for researchers to develop potent PPARs modulating anti-obesity agents. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"291 - 323"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42734545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promising Efficacy of Nanocurcumin in Comparison to Curcumin Against Nicotine-induced Complications","authors":"A. Samanta, K. Chattopadhyay, S. Biswas, B. Paul, B. Chattopadhyay","doi":"10.1080/22311866.2022.2120074","DOIUrl":"https://doi.org/10.1080/22311866.2022.2120074","url":null,"abstract":"Abstract Long-term exposure to nicotine severely affects the immune system. Nicotine-addicted women suffer more to diseases because of their history to several disorders or susceptibility to nicotine-related morbidity and mortality. The beneficial uses of nano-curcumin in nicotine-addicted women are still unknown, possibly due to its poor pharmacokinetics and limited bioavailability. Nanocurcumin is administered to study the immune-protective effects in nicotine exposed female rats. Female albino rats of Wistar strain (Rattus norvegicus) are nicotine injected (2.5 mg/kg bodyweights s.c., daily) for 21 days. Three hours later nanocurcumin is administered by oral gavages (4 mg/kg bodyweights/day). The dose and period of nanocurcumin is adopted from our earlier publications elsewhere. Animals are eradicated after the treatment period and immune-related cytokines, apoptotic markers, sex hormones and other parameters are measured from individual serum. Molecular docking, as well as, in vitro interactions of nanocurcumin with nicotine and some specific cellular proteins are measured for understanding the mechanism of action of nanocurcumin. Nanocurcumin reduced protein oxidation, augmented the immune system by regulating the expression of immunity-related cytokines and apoptotic markers. It helps maintaining the healthy levels of sex hormones and protein content of cells under nicotine-stressed condition. Molecular docking and in vitro interactions reveal that nanocurcumin possesses higher binding ability to nicotine and several cellular proteins. Cytokines, apoptotic markers and steroidogenic hormones are severely affected by nicotine exposure. Bio-soluble nanocurcumin acts as significant immune-modulator, which offers better protection than curcumin to female rats from nicotine-induced complications. Heightened therapeutic effects of nanocurcumin are discussed.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"366 - 377"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47075961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Enzyme Inhibition and In vivo Anti-hyperuricemic Potential of Malic Acid: An Experimental Approach","authors":"V. Vijeesh, A. Vysakh, N. Jisha, M. Latha","doi":"10.1080/22311866.2022.2124192","DOIUrl":"https://doi.org/10.1080/22311866.2022.2124192","url":null,"abstract":"Abstract The prevalence of hyperuricemia among adolescents in developed and developing countries is of great concern. Hyperuricemia is a metabolic disorder that intensifies the risk of serious illnesses such as gout, diabetes, cardiovascular, renal complications etc. This can be controlled through regulating the xanthine oxidase (XO) enzyme activity. Current XO inhibitors used for the treatment exert some severe side effects. Naturally derived molecules are better alternative to overcome these issues. Malic acid, a four-carbon dicarboxylic acid, is mostly present in plants and fruits and is utilised extensively in the diet, chemical, and pharmaceutical industries. The IC50 value obtained for malic acid was 3.81 ± 0.002 × 10 M and it is a good competitive inhibitor. An altered levels of various biological markers (renal, hepatic and haematological) were observed in potassium oxonate induced in vivo hyperuricemia model. When treated with malic acid (different doses of 25, 50 and 100 mg/kg bwt), altered levels of biological function markers retained close to their normal range. The current study revealed the anti-hyperuricemic potential of malic acid. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"344 - 352"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49410734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Antiviral Activity of Sulfated Polysaccharides Derived from Two Sargassum Species of Red Sea Egypt","authors":"G. Ismail, M. Hessien, M. A. Saleh, M. M. Ismail","doi":"10.1080/22311866.2022.2110517","DOIUrl":"https://doi.org/10.1080/22311866.2022.2110517","url":null,"abstract":"Abstract Brown seaweeds are known natural antioxidants and antiviral candidates. This study aimed to extract and characterize polysaccharides from Sargassum euryphyllum and Sargassum aquifolium brown macroalgal species and investigate their antiviral potential against avian infectious bronchitis virus (IBV). The chemical composition of the polysaccharides was characterized by Fourier transform infrared spectrometry, proton nuclear magnetic resonance, gel permeation chromatography, and high-performance liquid chromatography. Antioxidant activity was also determined as 2,2-diphenyl-1-picrylhydrazyl (DDPH) radical, hydrogen peroxide radical scavenging, and total antioxidant activity. Data revealed that S. aquifolium polysaccharides encompass significant ash (17.84% ± 0.69%), carbohydrates (57.43% ± 0.40%), sulfates (18.26% ± 0.52%), and uronic acids (45.40% ± 0.39%) content compared to S. euryphyllum polysaccharides. The molecular weight (MW) recorded 557 kDa for S. aquifolium polysaccharide with neutral sugars of mannose, galactose, and fructose, which constituted bulk molar ratios. A lower MW of 317 kDa was recorded for S. euryphyllum polysaccharide with mannose and xylose as the dominant neutral sugars. Both polysaccharides showed no cytotoxicity on chicken embryo kidney (CEK) cells. Potent antioxidant activity, measured as DPPH assay, for S. euryphyllum (59.15% ± 2.75%) and S. aquifolium (65.25% ± 2.89%) polysaccharides was established compared to ascorbic acid (67.33% ± 2.24%). The IBV titer on CEK cells in vitro showed a decreasing pattern from 5.4 to 4 log10 median tissue culture infectious dose (TCID50)/ml with S. euryphyllum polysaccharide and from 4.5 to 2.2 log10 TCID50/ml with S. aquifolium polysaccharide within 6 days post incubation time. A similar significant anti-IBV activity was estimated in vivo for both polysaccharides with a chorioallantoic membrane inoculation assay. Decreasing IBV titer suggested blocking of the virus entry, probably through disturbing its binding to the host cell receptors. The study recommended sulfated polysaccharides of S. aquifolium and S. euryphyllum seaweeds as potential antioxidants and antiviral natural resources. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"324 - 343"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47767346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Photoprotective Potentials and Chemical Comparison of Three Species of Ocimum","authors":"R. Piva, M. Verdan, C. Cardoso","doi":"10.1080/22311866.2022.2073268","DOIUrl":"https://doi.org/10.1080/22311866.2022.2073268","url":null,"abstract":"Abstract Several species of the genus Ocimum are traditionally known and consumed by the population, but studies on aqueous extract composition are restricted to preparations by infusion. The aim of this study was to compare three different aqueous extractive methods (decoction, infusion, and maceration) of the leaves from Ocimum basilicum, O. gratissimum, and O. kilimandscharicum, using their chemical composition, and antioxidant and photoprotective potentials. Using the data from LC-DAD-ESI-MS/MS, we identified two organic acids and ten flavonoids in the extracts. Comparing the three studied species, O. gratissimum showed the greatest antioxidant and photoprotective potentials and the highest concentration of phenolic substances, followed by the species O. basilicum and O. kilimandscharicum. Considering the extraction methods, decoction showed the greatest antioxidant and photoprotective potentials and the highest content of phenolic substances regardless of the species. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"12 1","pages":"243 - 253"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43770902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity evaluation of novel antidiabetic compound (11-methoxy-2-methyltridecane-4-ol) from marine macro alga, Gracilaria edulis","authors":"R. Vijayaraj, N. Sri Kumaran, K. Altaff","doi":"10.1080/22311866.2022.2069153","DOIUrl":"https://doi.org/10.1080/22311866.2022.2069153","url":null,"abstract":"Abstract Diabetic mellitus is considered to be a global public health issue with high prevalence in Asian countries including India. Pharmaceutical approach using a wide range of existing drugs is problematic because of their side effects, toxicity and resistance. Natural ingredients are promising for the treatment of Diabetic mellitus including seaweeds. This study evaluates the toxicity and safety of a novel antidiabetic compound 11-methoxy-2-methyltridecane-4-ol extracted and isolated from the marine macroalgae Gracilaria edulis. The toxicity impact of this compound was assessed using Artemia franciscana model, Red Blood Cells hemolysis assay (RBC), cytotoxicity assay using 3T3-L1 mouse preadipocytes cell line models and in vivo acute toxicity tests using rat (Rattus norvegicus) model. The cytotoxicity tests with 1000 µg/mL concentration of 11-methoxy-2-methyltridecane-4-ol compound on A. franciscana, red blood cell hemolysis and 3T3-L1 mouse preadipocytes cell line did not show any adverse effects. Acute toxicity study on hematological and biochemical parameters of experimental and control rats (Rattus norvegicus) at a dose of 500 mg/kg body weight recorded values within the normal range. The results of present study are indicative of the nontoxic nature and safety of this bioactive compound suggesting its utilization as a novel drug for long term treatment of Diabetes mellitus.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":"16 3","pages":"223 - 231"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41292191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}