抗炎活性指导下铁线莲地上部的分离。直流交货

Pub Date : 2022-09-03 DOI:10.1080/22311866.2022.2124193

Ritu Kalia, S. Jachak

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引用次数: 0

摘要

摘要铁线莲。不包括DC。也被称为印度旅行者的喜悦在印度的不同地区用于治疗皮肤感染、割伤和伤口。本研究采用70%甲醇提取物对三角菊地上部分及其正己烷、乙酸乙酯和正丁醇组分进行了研究。分别通过抑制LPS刺激的RAW 264.7巨噬细胞细胞系和卡拉胶诱导的体内大鼠爪水肿模型中的NO产生来评估体外和体内抗炎潜力。所有提取物和组分均表现出浓度依赖性抑制作用。结果表明，在100μg/mL浓度下，乙酸乙酯组分对NO释放的抑制率最高，为77.24±0.71%。在400mg/Kg的剂量下，C.gouriana的乙酸乙酯部分在卡拉胶诱导的大鼠爪水肿模型中也显示出显著的抗炎活性。此外，对具有生物活性的乙酸乙酯部分的植物化学研究导致分离出五种化合物，属于酚类和黄酮类天然产物。图形摘要

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Anti-inflammatory Activity Guided Fractionation of Aerial Parts of Clematis gouriana Roxb. ex DC

Abstract Clematis gouriana Roxb. ex DC. also known as Indian Traveler’s Joy is used in different regions of India for curing skin infections, cuts and wounds. The study was performed on 70% methanol extract of C. gouriana aerial parts and its n-hexane, ethyl acetate and n-butanol fractions. The in vitro and in vivo anti-inflammatory potential was evaluated using inhibition of NO production in LPS-stimulated RAW 264.7 macrophages cell line and carrageenan-induced rat paw edema model in vivo, respectively. All the extracts and fractions exhibited concentration-dependent inhibitory effects. It was found that ethyl acetate fraction was the most potent fraction showing 77.24±0.71% inhibition of NO release at 100 μg/mL concentration. The ethyl acetate fraction of C. gouriana also showed significant anti-inflammatory activity in the carrageenan-induced rat paw edema model at a dose of 400 mg/Kg. Further, the phytochemical investigation of the bioactive ethyl acetate fraction led to isolation of five compounds belonging to phenolics and flavonoids type of natural products. GRAPHICAL ABSTRACT

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