Journal of Biologically Active Products from Nature最新文献_第4页

Evaluation of in vitro and in vivo Anti-neuroinflammatory and Genotoxic Activities of the Alkaloid Tryptophol-5-O-β-D-glucopyranoside from Ocotea minarum 八角生物碱色氨酸5-O-β-D-吡喃葡糖苷的体内外抗炎和基因毒性活性评价

Journal of Biologically Active Products from Nature Pub Date : 2023-03-04 DOI: 10.1080/22311866.2023.2225482

Ana Paula de Araújo Boleti, Pedro Hernique de Oliveira Cardoso, Breno Frihling, Zaira da Rosa Guterres, Ana Francisca Gomes da Silva, A. A. A. de Rezende, F. Garcez, W. Garcez, U. Graf, M. Spanó, Lillian May Grespan Estodutto da Silva, L. Migliolo

{"title":"Evaluation of in vitro and in vivo Anti-neuroinflammatory and Genotoxic Activities of the Alkaloid Tryptophol-5-O-β-D-glucopyranoside from Ocotea minarum","authors":"Ana Paula de Araújo Boleti, Pedro Hernique de Oliveira Cardoso, Breno Frihling, Zaira da Rosa Guterres, Ana Francisca Gomes da Silva, A. A. A. de Rezende, F. Garcez, W. Garcez, U. Graf, M. Spanó, Lillian May Grespan Estodutto da Silva, L. Migliolo","doi":"10.1080/22311866.2023.2225482","DOIUrl":"https://doi.org/10.1080/22311866.2023.2225482","url":null,"abstract":"Abstract Ocotea minarum (Nees & Mart.) Mez is a tree belonging to the Lauraceae family and found in the Brazilian Cerrado ecosystem. Although the chemical composition of this species has already been described, little is known about the biological activities of one of the constituents of its fruits-the alkaloid tryptophol-5-O-β-D-glucopyranoside. In the present study, we assessed the anti-neuroinflammatory, mutagenic and recombinogenic properties of this indole alkaloid. Cytotoxicity and anti-inflammatory activities were performed using a model of LPS-stimulated microglia BV-2. Mutation detection and somatic recombination were performed in Drosophila melanogaster wing cells. The results showed that tryptophol-5-O-β-D-glucopyranoside was not cytotoxic to BV-2 cells at the concentrations tested. The results also showed that this alkaloid inhibited by 75% the inflammatory response induced by LPS at the concentration of 189 μM with IC50 = 12.07±0.35 μM. The analysis of the MH offspring showed that different concentrations of the alkaloid did not induce statistically significant increases in the frequencies of mutant spots when compared to those observed in the negative control. With these results, we can conclude that tryptophol-5-O-β-D-glucopyranoside presents therapeutic potential for the treatment of neurodegenerative diseases with no mutagenic effects. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41701248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile Constituents and In vitro Antimicrobial Activities of Essential Oils from Leaves of Siliquamomum oreodoxa N.S. Lý & Škorničk and Curcuma thorelii Gagnep. (Zingiberaceae) Growing in Vietnam 西葫芦叶和姜黄叶精油的挥发性成分和体外抗菌活性。（姜科）生长在越南

Journal of Biologically Active Products from Nature Pub Date : 2023-03-04 DOI: 10.1080/22311866.2023.2224286

Hieu Tran-Trung, L. Giang, D. Duc, Nguyen Thi Giang An, D. Van Son, Danh C. Vu, Nguyễn Thị Kim Anh, Hien Nguyen-Thi-Thu, Kh Tam, Trang H. D. Nguyen

引用次数: 1

Unveiling the Anti-cancer Potential of Bergenia ciliata (haw.) Sternb: A Mechanistic Study on UPR Modulation and ROS Generation 揭示纤毛菌的抗癌潜力(haw.)UPR调控和ROS生成的机制研究

Journal of Biologically Active Products from Nature Pub Date : 2023-03-04 DOI: 10.1080/22311866.2023.2220312

Ozaira Qadri, N. Hilal, K. Fazili

{"title":"Unveiling the Anti-cancer Potential of Bergenia ciliata (haw.) Sternb: A Mechanistic Study on UPR Modulation and ROS Generation","authors":"Ozaira Qadri, N. Hilal, K. Fazili","doi":"10.1080/22311866.2023.2220312","DOIUrl":"https://doi.org/10.1080/22311866.2023.2220312","url":null,"abstract":"Abstract Bergenia ciliata (haw.) Sternb, a well-known medicinal plant, has traditionally been used to treat various ailments, such as diabetes, microbial infections, and kidney stones, owing to its anti-inflammatory and anti-tussive properties. Here we aimed to evaluate the anti-cancer potential of the plant extract and deduce the molecular pathways involved. To investigate this, we used the MTT assay to determine the IC50 values of the methanolic extract of B. ciliata (BcME) in MDA-MB-231 and C6-Glioma cell lines. After treating the cancer cell lines with BcME for 18 hours, we evaluated the UPR signalling markers, including pIRE1, Xbp1, ATF6, eIF2α, ATF4, and CHOP, using Immunoblotting. We also used RT-PCR to determine the mRNA levels of ATF4 and performed enzyme assays using Spectrophotometric techniques to measure ROS levels. Our results indicate that B. ciliata has potent anti-cancer properties due to its ability to modulate UPR and ROS pathways. Specifically, the plant extract effectively repressed the cytoprotective UPR by blocking IRE1-Xbp1 and ATF6 pathways while enhancing the PERK-ATF4-CHOP pathway, which is known to switch UPR towards apoptosis. Furthermore, our study revealed that B. ciliata significantly caused the accumulation of ROS in cancer cells and inhibited the antioxidant enzymes catalase and superoxide dismutase. These results conclusively suggest that BcME works synergistically on both UPR and ROS pathways to promote apoptosis and eliminate cancerous cells and thus serves as a potential primary source for bioactive molecules, selectively targeting cancer cells. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46539026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Potential Immunomodulatory Properties of Noni Juice on Human Monocytes and Macrophages 诺尼果汁对人单核细胞和巨噬细胞潜在免疫调节特性的探讨

Journal of Biologically Active Products from Nature Pub Date : 2023-03-04 DOI: 10.1080/22311866.2023.2225480

Weslley Guimarães Bovi, C. S. Desidério, R. O. Trevisan, M. M. Santos, Mariana de Oliveira Silva, M. V. da Silva, V. R. Júnior, E. U. Bucek, C. J. Oliveira

{"title":"Exploring the Potential Immunomodulatory Properties of Noni Juice on Human Monocytes and Macrophages","authors":"Weslley Guimarães Bovi, C. S. Desidério, R. O. Trevisan, M. M. Santos, Mariana de Oliveira Silva, M. V. da Silva, V. R. Júnior, E. U. Bucek, C. J. Oliveira","doi":"10.1080/22311866.2023.2225480","DOIUrl":"https://doi.org/10.1080/22311866.2023.2225480","url":null,"abstract":"Abstract Noni, also known as Morinda citrifolia, is a plant that has been widely used for treating various pathologies due to its allegedly anti-inflammatory and immunomodulating properties. Macrophages and monocytes are critical immune cells that initiate inflammation and generate an immune response against various aggressors, regardless of their causative agent. However, it is not well investigated how noni fruit components affect the behavior or phenotype of these cells. Hence, studies in this direction could potentially demonstrate the therapeutic effects of this fruit, which is consumed worldwide. This study aimed to investigate the therapeutic properties of noni fruit juice in modulating the behavior and phenotype of human monocytes and macrophage cells in vitro. The results show that noni fruit juice inhibited the production of nitric oxide (NO) and inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-4, while stimulating the production of the anti-inflammatory cytokine IL-10 in monocytes. In addition, it negatively modulated the expression of toll-like receptor 4 (TLR4). In macrophages, noni fruit juice enhanced the secretion of all cytokines and NO, and inhibited the expression of TLR4. The contrasting results observed could be attributed to the joint stimuli of PMA (Phorbol 12-myristate 13-acetate), which was used to induce macrophage differentiation and noni Juice. Overall, the data indicate that noni may act as an anti-inflammatory agent and may aid in the treatment of uncontrolled inflammatory diseases as a complementary treatment to conventional therapies, under medical supervision. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41886976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant, Antimicrobial and Cytotoxic Activities of Amides and Aristolactams from Piper wallichii (Miq.) Hand.-Mazz. Stems Piper wallichii（Miq.）Hand酰胺和内酰胺的抗氧化、抗菌和细胞毒性活性-马兹。茎

Journal of Biologically Active Products from Nature Pub Date : 2023-03-04 DOI: 10.1080/22311866.2023.2211043

Nonthalert Lertnitikul, R. Suttisri, Supotchana Sitthigool, C. Pattamadilok, Kanyanat Piewpong, Ariya Khanboon, Jatupol Liangsakul, Cherdsak Boonyong

{"title":"Antioxidant, Antimicrobial and Cytotoxic Activities of Amides and Aristolactams from Piper wallichii (Miq.) Hand.-Mazz. Stems","authors":"Nonthalert Lertnitikul, R. Suttisri, Supotchana Sitthigool, C. Pattamadilok, Kanyanat Piewpong, Ariya Khanboon, Jatupol Liangsakul, Cherdsak Boonyong","doi":"10.1080/22311866.2023.2211043","DOIUrl":"https://doi.org/10.1080/22311866.2023.2211043","url":null,"abstract":"Abstract Four amides, namely, piperine (1), pellitorine (2), piperiline (5) and N-trans-p-coumaroyltyramine (7), and three aristolactams, i.e., piperolactams D (3), B (4) and A (6), were isolated from the methanol extract of Piper wallichii stems. Piperiline, piperolactams B and D were obtained from this plant for the first time. Compounds 1, 3, 5, 6 and 7 were evaluated for their antioxidant, antimicrobial and cytotoxic activities. Piperine (1) displayed the highest antioxidant activity in scavenging DPPH radicals with an IC50 value of 94.51 ± 11.91 μM. Piperolactams D (3) and A (6) showed antibacterial activity against Gram-positive bacteria (B. subtilis and S. aureus) with MICs of between 500-1000 μM. All test compounds were cytotoxic to breast cancer (MCF-7) cells, while the aristolactams were more toxic to colon cancer (Caco-2) cells than the amides. Compounds 1, 3, 6 and 7 were moderately cytotoxic to the doxorubicin-resistant MCF-7 subline (MCF-7/DOX). All compounds were non-toxic to normal human fibroblast (NIH/3T3) cells. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43901505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cytotoxic Activity of Alkaloids Isolated from Stephania glandulifera Miers 金银花生物碱的细胞毒活性研究

Journal of Biologically Active Products from Nature Pub Date : 2023-03-04 DOI: 10.1080/22311866.2023.2224283

Jhalnath Dhungel, B. Marasini, Erendra Manandhar, R. Rathnayaka, S. Samarakoon, S. Shyaula

{"title":"Cytotoxic Activity of Alkaloids Isolated from Stephania glandulifera Miers","authors":"Jhalnath Dhungel, B. Marasini, Erendra Manandhar, R. Rathnayaka, S. Samarakoon, S. Shyaula","doi":"10.1080/22311866.2023.2224283","DOIUrl":"https://doi.org/10.1080/22311866.2023.2224283","url":null,"abstract":"Abstract From the caudex of Stephania glandulifera Miers, five alkaloids were isolated using a cold percolation method with 90% methanol. The methanol extract was fractionated into three different fractions from where the alkaloids were purified using preparative HPLC. Structure elucidations were performed by modern spectral analysis including Mass and 1D and 2D-NMR spectroscopy. The purified compounds were tested against the LN-229 human brain glioblastoma cell line by performing a Sulforhodamine B assay (SRB). The isolated compounds were confirmed to be tetrahydropalmatine (1), palmatine (2), stepharanine (3), stepholidine (4), and stepharine (5). Compound 5 showed anticancer activity in the LN-229 human brain glioblastoma cell line with an IC50 value of 8.1 μg/mL. In this study, five alkaloids belonging to three classes namely proaporphine, protoberberine, and tetrahydroprotoberberine were isolated for the first time from Stephania glandulifera Miers. Among them, stepharine showed anticancer activity in the LN-229 human brain glioblastoma cell line which can be further developed as a potential anticancer agent. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45202806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Composition and Antiproliferative Activity of Rhizome and Frond Essential Oils of Anemia schimperiana subsp. wightiana, a Rare Fern Endemic to South India 刺花属贫血植物根、叶精油的化学成分及抗增殖活性研究。南印度特有的一种稀有蕨类植物

Journal of Biologically Active Products from Nature Pub Date : 2023-03-04 DOI: 10.1080/22311866.2023.2211046

S. A. Nair, Rajani Kurup Sukumaryamma Remadevi, Smrithi S. Lal, R. Raju, R. Antony, Sabulal Baby

{"title":"Chemical Composition and Antiproliferative Activity of Rhizome and Frond Essential Oils of Anemia schimperiana subsp. wightiana, a Rare Fern Endemic to South India","authors":"S. A. Nair, Rajani Kurup Sukumaryamma Remadevi, Smrithi S. Lal, R. Raju, R. Antony, Sabulal Baby","doi":"10.1080/22311866.2023.2211046","DOIUrl":"https://doi.org/10.1080/22311866.2023.2211046","url":null,"abstract":"Abstract Anemia schimperiana C.Presl subsp. wightiana (Gardener) Fraser-Jenkins is a rhizomatous fern endemic to south India. Its rhizome and frond (leaf) essential oils (EOs) were isolated and characterized by gas chromatographic techniques. Thirty compounds (99.1%) of the rhizome EO were characterized, with myrtenyl acetate (50.6%), α-pinene (26.0%), myrtenol (7.3%) and trans-pinocarveol (5.6%) as its major constituents. Twenty-four compounds (97.8%), with trans-pinocarveol (43.0%), pinocarvone (17.3%) and myrtenyl acetate (15.8%), were identified in the frond EO. In MTT assay, rhizome EO showed prominent cytotoxicity against C6 (CD50 86.0 µg/mL) and DLA (CD50 6.1 µg/mL) cells. Fluorescent microscopic and caspase-3 activity assays demonstrated apoptotic cell death induced by the rhizome EO in DLA cells. MTT assay of myrtenyl acetate, α-pinene and myrtenol against DLA cells displayed CD50s of 7.4, 0.9 and 20.5 µg/mL, respectively, revealing α-pinene as the prominent constituent causing antiproliferative activity of the rhizome EO. This is the first report on the chemistry and antiproliferative activity of this rare aromatic fern endemic to south India. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42983442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimization of Extraction Conditions using Response Surface Methodology and HPTLC Fingerprinting Analysis of Indian Propolis 响应面法优化提取条件及hplc指纹图谱分析

Journal of Biologically Active Products from Nature Pub Date : 2023-01-02 DOI: 10.1080/22311866.2023.2185681

S. Sankaran, R. Dubey, S. Lohidasan

{"title":"Optimization of Extraction Conditions using Response Surface Methodology and HPTLC Fingerprinting Analysis of Indian Propolis","authors":"S. Sankaran, R. Dubey, S. Lohidasan","doi":"10.1080/22311866.2023.2185681","DOIUrl":"https://doi.org/10.1080/22311866.2023.2185681","url":null,"abstract":"Abstract The potential of Indian propolis as a suitable therapeutic agent is still to be explored to the fullest. As variation in the chemical composition across different geographical regions has been a major concern, it is, therefore, worthwhile to systematically investigate, and assess the quality of Indian propolis and set nationalized standards. The propolis collected from three different regions were authenticated for the major plant source, followed by the evaluation of physicochemical properties. Different extraction methods were compared, with their influence on balsam content, polyphenol, and flavonoid content. The sample coded HAR using ultrasonication gave the highest extraction yield of 71.87%. Further, the process variables in the ultrasonication method (i.e. Amplitude, extraction time, the concentration of ethanol) at three levels were optimized using the Box-Behnken response design. The model was found significant with the concentration of ethanol having a maximum influence on the responses. The model was successfully verified by setting the amplitude at 48%, extraction time to 5.6 mins, and ethanol concentration to 87% v/v. The extraction efficiency of 83.86±1.17% was achieved with a substantial diminution of extraction time. The total phenolic, total flavonol, and total flavanone content of the optimized batch were 28.74±0.13 mgGAE/g, 4.66±0.78 mgQE/g, and 3.16±0.14 mgPE/g of propolis respectively. Further HPTLC fingerprint image of the optimized extract revealed the presence of quercetin, p-coumaric acid, kaempferol, chrysin, pinocembrin, and galangin. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47732979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer activity of Indonesian Melia azedarach L.: Phyto-chemistry, in vitro and in silico studies 印尼苦楝的抗癌活性：植物化学、体外和计算机研究

Journal of Biologically Active Products from Nature Pub Date : 2023-01-02 DOI: 10.1080/22311866.2023.2172079

A. S. Nugraha, Lilla Nur Firli, R. Hendra, P. Keller, Reza Yuridian Purwoko, H. Idrus, Rien Ritawidya, M. B. Febrian, I. Mahendra, A. Kurniawan, Alfian M. Forentin, V. Y. Susilo, C. E. Kusumaningrum, Y. Setiadi, H. Wongso

{"title":"Anticancer activity of Indonesian Melia azedarach L.: Phyto-chemistry, in vitro and in silico studies","authors":"A. S. Nugraha, Lilla Nur Firli, R. Hendra, P. Keller, Reza Yuridian Purwoko, H. Idrus, Rien Ritawidya, M. B. Febrian, I. Mahendra, A. Kurniawan, Alfian M. Forentin, V. Y. Susilo, C. E. Kusumaningrum, Y. Setiadi, H. Wongso","doi":"10.1080/22311866.2023.2172079","DOIUrl":"https://doi.org/10.1080/22311866.2023.2172079","url":null,"abstract":"Abstract This study describes the isolation of bioactive compounds and their in vitro anticancer activities from Melia azedarach L leaves from Karanglor, Karangan, Karanganom Klaten, Central Java-Indonesia. Isolated and characterised were the phenolics, kaempferol 7-O-rutinoside 1 and 4-methoxyresorcinol 3, The anticancer activity of the crude methanol extract of M. azedarach against a number cell lines (LNCaP, MDA-MB-231, and MCF-7) was found to be moderate; kaempferol derivatives were notable for their antiproliferative activities. Computational analysis, including molecular docking simulations and molecular dynamic studies, were used to investigate the feasibility of radiolabelled versions of 1 and 3 as theranostic agents targeting tyrosine kinase-type cell surface receptor HER2 in cancer - the iodinated derivatives 2 and 4, respectively, were used in this investigation as surrogates. The corresponding radioiodinated 2 and 4 were found to have high binding affinities of 10.1 and 5.7 kcal/mol, respectively, and have a good stability on the receptor. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47684035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hispolon, A Bioactive Compound from Phellinus linteus, Induces Apoptosis of Human Breast Cancer Cells Through the Modulation of Oxidative Stress and Autophagy 海司波龙是一种从白毛中提取的生物活性化合物，通过调节氧化应激和自噬诱导人乳腺癌症细胞凋亡

Journal of Biologically Active Products from Nature Pub Date : 2023-01-02 DOI: 10.1080/22311866.2023.2191994

Amorn Pangjantuk, Phongsakorn Chueaphromsri, Phongsakorn Kunhorm, Ruchee Phonchai, S. Chumkiew, Nipha Chaicharoenau-domrung, Parinya Noisa

{"title":"Hispolon, A Bioactive Compound from Phellinus linteus, Induces Apoptosis of Human Breast Cancer Cells Through the Modulation of Oxidative Stress and Autophagy","authors":"Amorn Pangjantuk, Phongsakorn Chueaphromsri, Phongsakorn Kunhorm, Ruchee Phonchai, S. Chumkiew, Nipha Chaicharoenau-domrung, Parinya Noisa","doi":"10.1080/22311866.2023.2191994","DOIUrl":"https://doi.org/10.1080/22311866.2023.2191994","url":null,"abstract":"Abstract Breast cancer (BC) is a major type of cancer found in women worldwide, accounting for more than 25% of all females diagnosed as BC patients. The BC treatment still needs to be highly effective with few adverse effects on the rest of the body. This study's objective was to investigate how hispolon enhanced anticancer activities and examine the underlying processes in human breast cancer cells. Hispolon was used to treat the HTB-26 cell lines. We investigated cell viability, intracellular reactive oxygen species (ROS) production, immunofluorescence straining, MDC assay, and RT-PCR. According to the findings, hispolon inhibited HTB-26 cells from proliferating and caused them to undergo apoptosis. When the mechanism of action of hispolon was investigated, it was discovered that BCL-2 expression was downregulated while pro-apoptotic genes including caspase-3, caspase-9, and BAX were upregulated. The level of intracellular ROS was also shown to be increased by hispolon, and the expression of antioxidant genes including SOD, GPX, and Catalase was found to be downregulated. The increase in the confocal fluorescence image of LC3I/II and the identification of upregulated autophagy-related genes, such as LC3I/II, ATG5, ATG10, and ATG12, served as indicators of the activation of autophagy. Therefore, our findings showed that hispolon had great potential for being an effective therapeutic agent and could cause cell death in breast cancer cells. GRAPHICAL ABSTRACT","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49196331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1