Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli

Pub Date : 2022-11-02 DOI:10.1080/22311866.2022.2163690

Tapia-Rodríguez Melvin Roberto, Vidal-Gutiérrez Max, González-Ortiz Alejandra, Robles-Zepeda Ramón Enrique, Vilegas Wagner, Ayala-Zavala Jesús Fernando, Velázquez-Guadarrama Norma, Torres-Moreno Heriberto, López-Silvestre Julio César

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Abstract

Abstract The objective of the present study was to determine the chemical profile, and the antimicrobial and antibiofilm effect of the Lophocereus schottii extract against uropathogenic Escherichia coli. A methanolic extract of L. schottii was analyzed by mass spectrometry (ESI-IT-MSn and APCI-IT-MSn) to determine the profile of bioactive compounds. The antimicrobial potential was assayed by the microdilution broth method. The effect of the extract on motility was also determined. Quorum sensing systems were evaluated using a biosensor with Chromobacterium violaceum. The prevention of biofilm formation and biofilm remotion were analyzed by the crystal violet method. The chemical analysis allows to identify 8 phenolic compounds, 2 alkaloids and 1 triterpene, most of these reported for the first time to this plant. L. schottii extract showed inhibitory and bactericidal activity against uropathogenic E. coli (MIC= 6.5 mg/mL and CMB= 13 mg/mL). In addition, the effect of L. schottii on motility was observed, since the concentration of 6.5 mg/mL reduced this process by 60%. With regard to the inhibition of the Quorum sensing system, L. schottii (6.5 mg/mL) reduce the production of violacein in 45%. The same concentration of the extract (6.5 mg/mL) resulted the most effective to prevent biofilm formation in 65%. Regarding the biofilm remotion the higher tested concentration (3 MIC) was the most effective, removing 84% of biomass. The results showed that L. schottii has a variety of bioactive compounds and antimicrobial and antibiofilm potential against uropathogenic E. coli. GRAPHICAL ABSTRACT

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肖氏Lophocereus schottii对尿路致病性大肠杆菌的化学成分、抑菌活性及抗菌膜活性研究

摘要：本研究的目的是测定苦苣苔提取物对尿路致病性大肠杆菌的化学性质、抗菌和抗生物膜作用。通过质谱法（ESI-IT MSn和APCI IT MSn）分析L.schottii的甲醇提取物，以确定生物活性化合物的图谱。采用微量稀释肉汤法测定其抗菌潜力。还测定了提取物对运动性的影响。使用紫色细菌生物传感器评估群体感测系统。采用结晶紫法对生物膜的形成和去除进行了分析。化学分析可以鉴定出8种酚类化合物、2种生物碱和1种三萜，其中大部分是首次报道。schottii提取物对尿路致病性大肠杆菌具有抑制和杀菌活性（MIC=6.5mg/mL，CMB=13mg/mL）。此外，由于6.5 mg/mL的浓度将这一过程减少了60%，因此观察到了L.schottii对运动性的影响。关于Quorum传感系统的抑制作用，L.schottii（6.5 mg/mL）可将紫曲霉素的产生减少45%。相同浓度的提取物（6.5mg/mL）对防止生物膜形成最有效，达65%。关于生物膜的去除，较高的测试浓度（3 MIC）是最有效的，去除了84%的生物量。结果表明，schottii具有多种生物活性化合物，对尿路致病性大肠杆菌具有抗菌和抗生物膜的潜力。图形摘要

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