Journal of Biomedical Optics最新文献

{"title":"Characterization of vascular patterns in endometrial cancer via optical resolution photoacoustic microscopy.","authors":"Lukai Wang, Yixiao Lin, Sanskar Thakur, Jinhua Xu, Quing Zhu","doi":"10.1117/1.JBO.31.4.045002","DOIUrl":"https://doi.org/10.1117/1.JBO.31.4.045002","url":null,"abstract":"<p><strong>Significance: </strong>Accurate classification of endometrial pathology is clinically challenging due to the heterogeneous and focal nature of precancerous and malignant lesions. Vascular remodeling is closely linked to tumor progression and may serve as a biomarker for malignancy.</p><p><strong>Aim: </strong>We aim to characterize a label-free optical-resolution photoacoustic microscopy (OR-PAM) approach for high-resolution imaging and quantitative characterization and separability assessment of endometrial vasculature.</p><p><strong>Approach: </strong>A custom-built OR-PAM system was used to image 34 fresh uterus samples with histologically confirmed diagnoses: normal, benign, endometrial intraepithelial neoplasia (EIN), and endometrial cancer (EC). Thirty-one quantitative vascular features were extracted from structural and spectral analyses of the photoacoustic data, and five statistically significant and minimally correlated features were selected for the separability assessment framework. A pairwise cosine similarity matrix based on these features was computed to construct a weighted similarity network, which was embedded into a two-dimensional (2D) space with a force-directed layout. A logistic regression boundary was applied to the 2D embedding to evaluate separability between normal/benign and EC/EIN clusters. A logistic regression classifier was developed from a cosine similarity matrix and cross-validated using a leave-one-out strategy.</p><p><strong>Results: </strong>The cosine-similarity network graph placed 39 of 40 images on the expected side of the separation boundary. The logistic regression classifier yielded an area under the ROC curve (AUC) of 0.943, demonstrating strong discrimination between normal/benign and EC/EIN groups.</p><p><strong>Conclusions: </strong>OR-PAM combined with imaging feature analysis enables robust differentiation of endometrial pathologies and demonstrates potential as a noninvasive optical biopsy tool for endometrial assessment.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 4","pages":"045002"},"PeriodicalIF":2.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative assessment of rheumatoid arthritis treatment response using short-wave infrared hyperspectral imaging soft abundance scoring.","authors":"Hsin-Hua Chen, Hsin-Che Wang, Kuo-Lung Lai, Chiu-Chin Sung, Jun-Peng Chen, Yu-Wen Fu, Shao-Jen Weng, Hsian-Min Chen","doi":"10.1117/1.JBO.31.4.045001","DOIUrl":"https://doi.org/10.1117/1.JBO.31.4.045001","url":null,"abstract":"<p><strong>Significance: </strong>Non-invasive optical measures for evaluating rheumatoid arthritis (RA) remain limited. Clinical scores such as the 28-joint Disease Activity Score (DAS28) and ultrasound are operator-dependent and do not directly quantify optical tissue changes. Short-wave infrared (SWIR) hyperspectral imaging (HSI) provides sensitivity to inflammation-associated spectral changes in biological tissues.</p><p><strong>Aim: </strong>The aim is to develop and evaluate a quantitative HSI soft abundance scoring (HSISAS) method for assessing treatment response in RA patients using SWIR-HSI.</p><p><strong>Approach: </strong>Eleven RA patients who met the 2010 American College of Rheumatology/European Alliance of Associations for Rheumatology criteria underwent SWIR-HSI (900 to 1700 nm) of the wrist joints before and after 12 weeks of biologic therapy. The HSISAS algorithm used pixel-wise reflectance spectra together with spectral correlation structure estimation and constrained energy minimization-based abundance scoring to compute intra- and inter-subject scores. These scores were statistically compared with conventional clinical indices, including DAS28, erythrocyte sedimentation rate, C-reactive protein, and power Doppler ultrasound findings.</p><p><strong>Results: </strong>Both intra- and inter-HSISAS significantly increased after treatment ( <math><mrow><mi>p</mi> <mo><</mo> <mn>0.001</mn></mrow> </math> ) and were positively associated with DAS28 improvement. Reflectance spectra showed visible pre/post-treatment differences, particularly near 1250 nm. The abundance maps visually differentiated pre- and post-treatment states consistent with clinical improvement.</p><p><strong>Conclusions: </strong>The HSISAS framework provides a quantitative, non-contact optical measure of treatment-related spectral changes in RA. This method may offer translational potential for disease monitoring and may complement existing ultrasound and laboratory assessments.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 4","pages":"045001"},"PeriodicalIF":2.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective on the current state of hyperspectral/multispectral imaging for minimally invasive surgery.","authors":"Mark Witteveen, Cyrille Mooij, Behdad Dashtbozorg, Theo Ruers","doi":"10.1117/1.JBO.31.3.030601","DOIUrl":"https://doi.org/10.1117/1.JBO.31.3.030601","url":null,"abstract":"<p><strong>Significance: </strong>Minimally invasive surgery (MIS) offers substantial benefits to patients, including reduced trauma and faster recovery. However, it limits visual and tactile feedback, which can affect surgical decision-making. Hyperspectral and multispectral imaging (HSI/MSI) are imaging technologies with the potential to provide detailed, real-time tissue characterization, enhancing minimally invasive intraoperative guidance.</p><p><strong>Aim: </strong>This perspective aims to summarize the current state-of-the-art in the use of HSI/MSI in laparoscopy and endoscopy. It focuses on both technological development and clinical implementation, providing an overview of performance characteristics and translational state.</p><p><strong>Approach: </strong>A structured literature search was conducted to identify relevant studies. These were analyzed for key system specifications (spectral range, resolution, and acquisition speed) and clinical applications. The studies were further categorized using a \"bench-to-bedside\" framework to evaluate their level of translational progress.</p><p><strong>Results: </strong>The majority of studies fall within early translational stages (T1 to T2), with no reports of large-scale clinical trials. Most systems operate in the visible spectrum (450 to 650 nm), optimized for blood-related imaging. Applications include perfusion assessment, nerve visualization using short-wave infrared wavelengths, and tumor detection.</p><p><strong>Conclusions: </strong>HSI/MSI for MIS is a rapidly developing field with demonstrated potential across a range of applications. Continued research and validation are essential to transition these technologies from experimental use to routine surgical practice.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 3","pages":"030601"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13002860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147498841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure of tendon causes highly optical anisotropic properties and transport.","authors":"Alexa Nazarian, Steven L Jacques, Joshua Tam, Richard Rox Anderson, Sandra J Shefelbine","doi":"10.1117/1.JBO.31.3.035003","DOIUrl":"10.1117/1.JBO.31.3.035003","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Significance: &lt;/strong&gt;Tendons are highly anisotropic tissues that exhibit distinct optical properties depending on the direction of light propagation relative to their fiber orientation. Understanding these variations and how to modify them through optical clearing techniques is beneficial for many light-based applications, including photobiomodulation therapy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;To quantify how tendon optical transport depends on fiber orientation and wavelength, comparing light propagation parallel and perpendicular to the tendon's long axis, the axis along which collagen fibers are primarily aligned, and to evaluate glycerol-based optical clearing for increasing light penetration.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Approach: &lt;/strong&gt;The reduced scattering coefficient ( &lt;math&gt; &lt;mrow&gt; &lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;μ&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mi&gt;s&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mo&gt;'&lt;/mo&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;/mrow&gt; &lt;/math&gt; ) was measured from 400 to 1600 nm in tendon samples oriented parallel to the tendon's fiber axis (transverse slices) and perpendicular (longitudinal slices) to it. Diffuse reflectance and total transmittance were measured using an integrating sphere and spectrophotometer, and optical coefficients were derived through a theoretical Monte Carlo model. Angular scattering measurements at 633 nm were performed to characterize forward scattering behavior. Power transmission was measured in centimeter-scale tendon sections, and Monte Carlo simulations using the measured optical properties were used to model the transmission experiment and compare orientation-dependent penetration. Measurements were conducted in samples treated with saline or glycerol (10% and 60%). Beam profiler images were also captured to assess light distribution.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Longitudinal tendon sections exhibited consistently higher &lt;math&gt; &lt;mrow&gt; &lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;μ&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mi&gt;s&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mo&gt;'&lt;/mo&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;/mrow&gt; &lt;/math&gt; values than transverse sections. At 800 nm, &lt;math&gt; &lt;mrow&gt; &lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;μ&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mi&gt;s&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mo&gt;'&lt;/mo&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;/mrow&gt; &lt;/math&gt; was &lt;math&gt;&lt;mrow&gt;&lt;mn&gt;9.01&lt;/mn&gt; &lt;mtext&gt;  &lt;/mtext&gt; &lt;msup&gt;&lt;mrow&gt;&lt;mi&gt;mm&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mo&gt;-&lt;/mo&gt; &lt;mn&gt;1&lt;/mn&gt;&lt;/mrow&gt; &lt;/msup&gt; &lt;/mrow&gt; &lt;/math&gt; (longitudinal) versus &lt;math&gt;&lt;mrow&gt;&lt;mn&gt;0.57&lt;/mn&gt; &lt;mtext&gt;  &lt;/mtext&gt; &lt;msup&gt;&lt;mi&gt;mm&lt;/mi&gt; &lt;mrow&gt;&lt;mo&gt;-&lt;/mo&gt; &lt;mn&gt;1&lt;/mn&gt;&lt;/mrow&gt; &lt;/msup&gt; &lt;/mrow&gt; &lt;/math&gt; (transverse). Immersion in 60% glycerol greatly reduced &lt;math&gt; &lt;mrow&gt; &lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;μ&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mi&gt;s&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mo&gt;'&lt;/mo&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;/mrow&gt; &lt;/math&gt; in both orientations and increased transmitted power. Beam profiler images showed higher intensity in fascicles and fibers relative to the surrounding matrix. This structural pattern diminished with 60% glycerol treatment.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Tendons have strong anisotropic optical properties that favor optical propagation along their fiber axis. Glycerol increases light penetration depths, though","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 3","pages":"035003"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13021904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vivo</i> photoacoustic imaging of swine ureters injected with methylene blue.","authors":"Junior Arroyo, Junhao Zhang, Jiaxin Zhang, Nethra Venkatayogi, Manik Kakkar, Amanda Maxwell, Kathleen Gabrielson, Muyinatu A Lediju Bell","doi":"10.1117/1.JBO.31.3.036004","DOIUrl":"10.1117/1.JBO.31.3.036004","url":null,"abstract":"<p><strong>Significance: </strong>Ureteral injuries represent a major concern during a range of surgical procedures, due to the proximity of the ureter to target surgical structures. Intraoperative identification of the ureter is critical to prevent this accidental damage.</p><p><strong>Aim: </strong>We demonstrate the first known <i>in vivo</i> photoacoustic imaging of the ureter in swine following intravenous administration of FDA-approved methylene blue, enabled by a software-hardware integration that has not been previously reported in the literature.</p><p><strong>Approach: </strong>Photoacoustic channel data from the ureters of two swine were acquired using a Vevo F2 ultrasound system and an Opotek Phocus Mobile laser. Images were beamformed using a delay-and-sum algorithm. Photoacoustic image quality was evaluated using contrast, signal-to-noise ratio (SNR), and generalized contrast-to-noise ratio (gCNR) metrics, measured 10 to 80 min after methylene blue injection.</p><p><strong>Results: </strong>Across the 10- to 80-min imaging window, median contrast (3.46-11.43 dB), SNR (2.84-6.99), and gCNR (0.27-0.64) confirmed sustained ureter visibility with methylene blue. Maximum image quality was observed 20- to 30-min after methylene blue injection, with significantly higher contrast, SNR, and gCNR values compared with earlier or later time points ( <math><mrow><mi>p</mi> <mo><</mo> <mn>0.05</mn></mrow> </math> ).</p><p><strong>Conclusions: </strong><i>In vivo</i> results demonstrate that methylene-blue-enhanced photoacoustic imaging can visualize the ureter over a time duration that is consistent with the length of surgical procedures, providing initial feasibility for real-time photoacoustic-guided surgery applications.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 3","pages":"036004"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12988832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re: Dao et al., <i>In situ</i> observation of urothelial responses to NaCl-induced osmotic stress using optical coherence tomography.","authors":"Pradeep Tyagi","doi":"10.1117/1.JBO.31.3.039701","DOIUrl":"10.1117/1.JBO.31.3.039701","url":null,"abstract":"<p><p>The letter comments on a JBO article by Dao et al.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 3","pages":"039701"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12949945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Color-filter-array-based multispectral photoplethysmography optical sensor and its motion artifact correction algorithm.","authors":"Yunfen Wei, Shu Cong, Runchao Yan, Zekai He, Hong Li, Gongming Yu, Mei Zou","doi":"10.1117/1.JBO.31.3.037001","DOIUrl":"https://doi.org/10.1117/1.JBO.31.3.037001","url":null,"abstract":"<p><strong>Significance: </strong>The performance of wearable biosensors is highly influenced by motion artifacts (MAs).</p><p><strong>Aim: </strong>We propose a motion artifact removal algorithm using blind source separation-multi-stage least mean square adaptive filtering with multi-wavelength photoplethysmography (PPG) signals to enable accurate physiological parameter estimation in wearable devices.</p><p><strong>Approach: </strong>The algorithm is implemented with a custom-designed PPG sensor that enables synchronized multi-wavelength acquisition via a compact optical design integrated with a color filter array. The algorithm exploits the high correlation of MA components across wavelengths to autonomously generate a noise reference in real time through blind source separation. Furthermore, a frame-level quality assessment mechanism based on power spectral entropy is introduced, which dynamically evaluates the interference level according to the entropy value and intelligently switches between two pre-optimized sets of filter parameters. This allows for dynamic parameter adjustment of the MSLMS filter, thereby effectively tracking and suppressing motion artifacts without the need for external inertial sensors.</p><p><strong>Results: </strong>The performance of the proposed algorithm was evaluated in a study involving 13 subjects performing free-arm swings to simulate daily motion. Experimental results demonstrate that after algorithm processing, the limits of agreement between the estimated heart rate and the electrocardiogram reference values narrowed from [3.09, 26.94] to <math><mrow><mo>[</mo> <mo>-</mo> <mn>1.98</mn> <mo>,</mo> <mn>2.11</mn> <mo>]</mo> <mtext>  </mtext> <mi>bpm</mi></mrow> </math> , the Pearson correlation coefficient improved from 0.86 to 0.99, and the mean absolute error significantly decreased from 15.12 to 0.76 bpm.</p><p><strong>Conclusions: </strong>We present an integrated hardware-algorithm co-design, offering a practical solution for high-precision and robust physiological monitoring in ambulatory settings using wearable devices.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 3","pages":"037001"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12989444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147474051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free correlative morpho-chemical tomography of 3D kidney mesangial cells.","authors":"Ankit Butola, Biswajoy Ghosh, Jaena Park, Minsung Kwon, Alejandro De la Cadena, Sudipta S Mukherjee, Rohit Bhargava, Stephen A Boppart, Krishna Agarwal","doi":"10.1117/1.JBO.31.3.036501","DOIUrl":"10.1117/1.JBO.31.3.036501","url":null,"abstract":"<p><strong>Significance: </strong>Imaging 3D <i>in vitro</i> kidney models is essential to understand kidney function and pathology. Label-free characterization of such specimens seeks to supplement existing imaging techniques and avoid the need for contrast agents that can disturb the native state of living samples. Conventional label-free optical imaging techniques are compatible with living samples but face challenges such as poor sectioning capability, fragmentary morphology, and lack of chemical-specific information.</p><p><strong>Aim: </strong>We aim to develop and demonstrate a correlative label-free imaging platform capable of simultaneously capturing morphological and chemical-specific information from 3D cultured kidney mesangial cells.</p><p><strong>Approach: </strong>We combined simultaneous label-free autofluorescence-multiharmonic (SLAM) microscopy and gradient light interference microscopy (GLIM) to extract both chemical-specific and morphological tomography of 3D cultured kidney mesangial cells. In this approach, SLAM provides a nonlinear imaging platform with a single excitation source to simultaneously acquire autofluorescence (FAD and NAD(P)H), second- and third-harmonic signals from the cells. Complementarily, GLIM acquires high-contrast quantitative phase information to quantify structural changes in samples with a thickness of up to <math><mrow><mn>250</mn> <mtext>  </mtext> <mi>μ</mi> <mi>m</mi></mrow> </math> .</p><p><strong>Results: </strong>Our correlative imaging results demonstrate the ability to image and quantify both morphology and chemical-specific signals of kidney mesangial cells in 3D. The combination of GLIM and SLAM provides complementary information critical for understanding kidney function, including metabolism and matrix deposition under controlled physiological conditions.</p><p><strong>Conclusions: </strong>The proposed correlative imaging approach establishes a versatile and hassle-free platform for morpho-chemical cellular tomography, offering unique opportunities for studying the structure and function of 3D kidney models in their native state.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 3","pages":"036501"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12972753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Learning to simulate realistic human diffuse reflectance spectra (Erratum).","authors":"Marco Hübner, Ahmad Bin Qasim, Alexander Studier-Fischer, Maike Rees, Viet Tran Ba, Jan-Hinrich Nölke, Silvia Seidlitz, Jan Sellner, Janne Heinecke, Jule Brandt, Berkin Özdemir, Kris Dreher, Alexander Seitel, Felix Nickel, Caelan Max Haney, Karl-Friedrich Kowalewski, Leonardo Ayala, Lena Maier-Hein","doi":"10.1117/1.JBO.31.3.039801","DOIUrl":"https://doi.org/10.1117/1.JBO.31.3.039801","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1117/1.JBO.31.2.026004.].</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 3","pages":"039801"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13038045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147592386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive study on the characterization of lysed blood samples using dual-wavelength photoacoustics.","authors":"Subhadip Paul, Hari Shankar Patel, Vatsala Misra, Ravi Rani, Amaresh K Sahoo, Ratan K Saha","doi":"10.1117/1.JBO.31.3.035001","DOIUrl":"10.1117/1.JBO.31.3.035001","url":null,"abstract":"<p><strong>Significance: </strong>Anemia is a global health concern, prompting the need for rapid, accurate, and noninvasive diagnostic tools. This has led to significant interest in the development of various optical tools, including photoacoustic (PA) spectroscopy for monitoring and quantification of clinically relevant blood parameters.</p><p><strong>Aim: </strong>Estimating the blood lysis level (LL) and oxygenation ( <math> <mrow><msub><mi>SO</mi> <mn>2</mn></msub> </mrow> </math> ) is essential for the detection of various hemolytic conditions, including anemia. The PA spectroscopy is explored here for quantifying hemolytic blood samples.</p><p><strong>Approach: </strong><i>In vitro</i> PA measurements on human blood samples were validated through simulation studies involving discrete dipole approximation, Monte Carlo, and k-Wave methods. Blood hematocrit (H), LL, and <math> <mrow><msub><mi>SO</mi> <mn>2</mn></msub> </mrow> </math> levels are determined from simulated and experimental PA signals.</p><p><strong>Results: </strong>The wavelength pairs 700-905 and 700-1000 nm are found to be optimal for the estimation of H and <math> <mrow><msub><mi>SO</mi> <mn>2</mn></msub> </mrow> </math> with high accuracy ( <math><mrow><mo>></mo> <mn>90</mn> <mo>%</mo></mrow> </math> ). The correlation coefficient between the actual and evaluated LLs is calculated to be <math><mrow><mo>≈</mo> <mn>0.90</mn></mrow> </math> .</p><p><strong>Conclusions: </strong>Results show that PA measurements with a suitable combination of optical wavelengths can be used for determining the important blood parameters accurately and simultaneously. Further investigation is needed to apply the developed method under an <i>in vivo</i> setting.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"31 3","pages":"035001"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12950612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}