Journal of Biomedical Optics最新文献

筛选
英文 中文
Thermal damage induced changes in optical properties of the porcine dermis. 热损伤引起猪真皮光学特性的变化。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-10-01 Epub Date: 2025-10-11 DOI: 10.1117/1.JBO.30.10.105003
Anjelyka T Fasci, Maria A T Hoffman, Andrea L Smith, Matthew E Macasadia, Amanda J Tijerina, Robert Lyle Hood, Michael P DeLisi, Joel N Bixler
{"title":"Thermal damage induced changes in optical properties of the porcine dermis.","authors":"Anjelyka T Fasci, Maria A T Hoffman, Andrea L Smith, Matthew E Macasadia, Amanda J Tijerina, Robert Lyle Hood, Michael P DeLisi, Joel N Bixler","doi":"10.1117/1.JBO.30.10.105003","DOIUrl":"10.1117/1.JBO.30.10.105003","url":null,"abstract":"<p><strong>Significance: </strong>Understanding thermal effects on tissue optical properties is fundamental for optimizing laser-based medical interventions. We address the critical knowledge gap of temperature-dependent changes in porcine dermis optical properties.</p><p><strong>Aim: </strong>We explore the thermal damage influence on the excised dermis optical properties at wavelengths from 400 to 1100 nm.</p><p><strong>Approach: </strong>Using a double-integrating-sphere system and inverse adding-doubling, we determined absorption, <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> , and reduced scattering, <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> , coefficients before and after a 2.5-min thermal exposure.</p><p><strong>Results: </strong>We observed non-linear changes in both <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> and <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> across temperature regimes. Minimal changes occurred at 37°C and 43°C. At 50°C, slight increases in both coefficients were observed. Significant alterations occurred at 60°C, with substantial increases in <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> and variable changes in <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> depending on wavelength region. At 70°C, <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> values remained elevated, whereas <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> showed mixed responses, with some wavelength regions decreasing, indicating progressive structural breakdown. The Arrhenius damage model showed an exponential increase with temperature.</p><p><strong>Conclusions: </strong>We reveal complex thermal-induced changes in tissue optical properties, particularly at higher temperatures. Findings reinforce a critical threshold between 50°C and 60°C where significant changes occur. The non-linear, wavelength-dependent responses emphasize the need for comprehensive data in laser-tissue interaction modeling, with important implications for optimizing laser-based medical treatments.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 10","pages":"105003"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of sample handling protocols on soft tissue attenuation coefficient and morphology. 样品处理方案对软组织衰减系数和形态的影响。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-10-01 Epub Date: 2025-10-14 DOI: 10.1117/1.JBO.30.10.106004
Freja Hoeier, Gavrielle R Untracht, Amanda Oester Andersen, Karina Straede, Andreas Kjaer, Peter E Andersen
{"title":"Impact of sample handling protocols on soft tissue attenuation coefficient and morphology.","authors":"Freja Hoeier, Gavrielle R Untracht, Amanda Oester Andersen, Karina Straede, Andreas Kjaer, Peter E Andersen","doi":"10.1117/1.JBO.30.10.106004","DOIUrl":"10.1117/1.JBO.30.10.106004","url":null,"abstract":"<p><strong>Significance: </strong>The use of tissue attenuation coefficients as biomarkers for disease detection is rising. However, especially for <i>ex vivo</i> studies, sample handling methods can notably impact tissue optical attenuation properties, and these effects have yet to be studied in detail.</p><p><strong>Aim: </strong>We aim to compare and evaluate common methods for sample handling and assess their impact on the optical attenuation and structural properties of <i>ex vivo</i> colon tissue.</p><p><strong>Approach: </strong>Six different handling methods were tested: Direct freezing at <math><mrow><mo>-</mo> <mn>80</mn> <mo>°</mo> <mi>C</mi></mrow> </math> , slow freezing in a cryobox with and without cryopreservation media, snap freezing in isopentane, formalin fixation, and fresh tissue stored directly in phosphate-buffered saline. All samples were imaged using optical coherence tomography; images were assessed qualitatively for morphological changes and quantitatively by extracting the tissue attenuation coefficient using the Lambert-Beer law. All handling methods were compared with representative histology (hematoxylin and eosin staining and periodic acid-Schiff staining).</p><p><strong>Results: </strong>All sample handling methods showed a significant difference in tissue attenuation and morphology relative to the fresh tissue ( <math><mrow><mi>p</mi> <mo>≪</mo> <mn>0.0001</mn></mrow> </math> ), with frozen samples generally showing a lower attenuation coefficient, e.g., directly frozen ( <math><mrow><mn>2.0</mn> <mo>±</mo> <mn>1.0</mn> <mtext>  </mtext> <msup><mrow><mi>mm</mi></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </mrow> </math> ) compared with formalin-fixed ( <math><mrow><mn>2.5</mn> <mo>±</mo> <mn>1.3</mn> <mtext>  </mtext> <msup><mrow><mi>mm</mi></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </mrow> </math> ) and fresh tissue ( <math><mrow><mn>2.5</mn> <mo>±</mo> <mn>1.0</mn> <mtext>  </mtext> <msup><mrow><mi>mm</mi></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </mrow> </math> ). Formalin-fixed and snap frozen samples had the smallest effect size ( <math><mrow><mi>δ</mi> <mo>=</mo> <mn>0.002</mn></mrow> </math> and <math><mrow><mo>-</mo> <mn>0.09</mn></mrow> </math> , respectively). Macroscopic structural changes were also observed, including alterations to the epithelial layer and indications of goblet cell degradation for all methods but formalin fixation.</p><p><strong>Conclusions: </strong>Understanding the impact of sample handling methods is critical to the accurate interpretation of morphology-based analysis. In the case of fresh tissue being unavailable, formalin-fixed and snap frozen tissue samples yield the best alternative with negligible effect sizes for colon tissue.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 10","pages":"106004"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12519020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145300820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blood component analysis using mid-infrared photoacoustic spectroscopy based on ultrasound detection: glucose analysis trial. 基于超声检测的中红外光声光谱血液成分分析:葡萄糖分析试验。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-10-01 Epub Date: 2025-10-09 DOI: 10.1117/1.JBO.30.10.107001
Kiiko Aiba, Saiko Kino, Yuji Matsuura
{"title":"Blood component analysis using mid-infrared photoacoustic spectroscopy based on ultrasound detection: glucose analysis trial.","authors":"Kiiko Aiba, Saiko Kino, Yuji Matsuura","doi":"10.1117/1.JBO.30.10.107001","DOIUrl":"10.1117/1.JBO.30.10.107001","url":null,"abstract":"<p><strong>Significance: </strong>Noninvasive monitoring of blood components is important in daily health management. Conventional optical techniques such as attenuated total reflection (ATR) have limited penetration depth and sensitivity. Photoacoustic spectroscopy using a piezoelectric transducer (PZT-PAS) can detect components in the interstitial fluid beneath the stratum corneum with a relatively simple device.</p><p><strong>Aim: </strong>We explored the feasibility of PZT-PAS for noninvasive analysis of blood components.</p><p><strong>Approach: </strong>Biomimetic phantom experiments were conducted to evaluate depth sensitivity. A technique for enhancing signal strength by generating standing acoustic waves in tissue was validated by tuning the laser modulation frequency. Human trials were conducted to assess the capability of the method for predicting blood glucose levels.</p><p><strong>Results: </strong>PZT-PAS successfully detected signals from depths beyond 10 to <math><mrow><mn>20</mn> <mtext>  </mtext> <mi>μ</mi> <mi>m</mi></mrow> </math> , outperforming ATR. Signal enhancement was achieved in a 2-mm-thick interdigital membrane using resonant acoustic conditions. In human trials, discriminant analysis to determine blood glucose levels relative to a threshold of 140 mg/dL showed an accuracy rate of 85.3%.</p><p><strong>Conclusions: </strong>These results highlight the potential of PZT-PAS combined with signal processing for future wearable, noninvasive health care monitoring applications.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 10","pages":"107001"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Digital holographic microscopy for rapid bacteria segmentation and counting in microfluidic cartridges: basic considerations and limitations for diagnostic application. 微流控墨盒中用于快速细菌分割和计数的数字全息显微镜:诊断应用的基本考虑和限制。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-10-01 Epub Date: 2025-10-15 DOI: 10.1117/1.JBO.30.10.106501
Hussein Kamel, Julian Schmid, Moaaz Rauf Nizami, Igor Alekseenko, Florian Hausladen, Daniel Claus, Rainer Wittig, Damien P Kelly
{"title":"Digital holographic microscopy for rapid bacteria segmentation and counting in microfluidic cartridges: basic considerations and limitations for diagnostic application.","authors":"Hussein Kamel, Julian Schmid, Moaaz Rauf Nizami, Igor Alekseenko, Florian Hausladen, Daniel Claus, Rainer Wittig, Damien P Kelly","doi":"10.1117/1.JBO.30.10.106501","DOIUrl":"10.1117/1.JBO.30.10.106501","url":null,"abstract":"<p><strong>Significance: </strong>Digital holographic microscopy (DHM) has proven effective for particle segmentation within a given volume, making it well-suited for rapid monitoring of bacterial growth in microfluidic cartridges-such as in single-cell-based antimicrobial susceptibility testing assays. However, the development of optimal assays depends on a range of factors related to the instrument, consumables, and the sample itself. Despite this, comprehensive investigations into how these parameters influence the quality of the resulting phase images remain limited.</p><p><strong>Aim: </strong>To address this problem, we systematically explore the effect of these factors, including the microfluidic chamber height and its material properties, the density of the suspension, and other sample-inherent properties, on the signal-to-noise ratio (SNR) of the reconstructed phase image.</p><p><strong>Approach: </strong>We constructed an off-axis digital holographic microscope and defined a robust numerical processing pipeline allowing for the numerical reconstruction, refocusing and counting of suspended particles in a measurement volume spanning roughly <math><mrow><mn>120</mn> <mo>×</mo> <mn>120</mn> <mo>×</mo> <mn>400</mn> <mtext>  </mtext> <mi>μ</mi> <msup><mrow><mi>m</mi></mrow> <mrow><mn>3</mn></mrow> </msup> </mrow> </math> , at 50× magnification. We analyzed the performance of this system using various dilution steps of silica microspheres, Gram-positive spherical <i>Staphylococcus warneri</i> and Gram-negative rod-shaped <i>Escherichia coli</i> bacteria, filled in commercial microfluidic chips with different chamber heights.</p><p><strong>Results: </strong>Experimental results demonstrated the system's capability in reflecting the dilution steps over 2 to 3 orders of magnitude. Our SNR analysis highlighted the microfluidic chamber height and the density of the suspension as key contributors to the background noise, whereas the particles themselves seemed to have a negligible effect. From this insight, we were able to derive an analytical function to predict the SNR of a given DHM system for various concentrations, chamber heights, and particle types.</p><p><strong>Conclusions: </strong>We successfully built a DHM system for counting suspended particles over a wide concentration range and for various microfluidic chamber heights. We also derived an initial framework for predicting and optimizing the performance of a given DHM system.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 10","pages":"106501"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12525663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Handheld multispectral photoacoustic imaging for assessing myocardial metabolism. 手持式多光谱光声成像评估心肌代谢。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-10-01 Epub Date: 2025-10-07 DOI: 10.1117/1.JBO.30.10.105001
Bokang Zhai, Yawen Shi, Handi Deng, Hongli Liu, Chenliang Xie, Naiyue Zhang, Wenyuan Yu, Dingce Sun, Yang Yu, Cheng Ma
{"title":"Handheld multispectral photoacoustic imaging for assessing myocardial metabolism.","authors":"Bokang Zhai, Yawen Shi, Handi Deng, Hongli Liu, Chenliang Xie, Naiyue Zhang, Wenyuan Yu, Dingce Sun, Yang Yu, Cheng Ma","doi":"10.1117/1.JBO.30.10.105001","DOIUrl":"10.1117/1.JBO.30.10.105001","url":null,"abstract":"<p><strong>Significance: </strong>Myocardial oxygen metabolism is a key focus of cardiac surgery. It serves as important evidence for surgeons to evaluate surgical quality and surgical plans. However, current clinical methods lack the capability to directly monitor dynamic changes in myocardial metabolism during surgery. Photoacoustic imaging (PAI), a biomedical optical imaging modality, offers real-time assessment of blood oxygen saturation. By visualizing oxygen saturation levels in both blood and muscle tissue, PAI provides a means to infer myocardial metabolic status intraoperatively.</p><p><strong>Aim: </strong>We use PAI to observe the differences between infarcted myocardium and normal cardiac muscle and to explore the feasibility of using PAI to monitor myocardial metabolism levels during cardiac surgery.</p><p><strong>Approach: </strong>Ten rabbits were randomly divided into experimental and control groups. The animals in the experimental group underwent thoracotomy followed by left anterior descending coronary artery ligation, whereas those in the control group received thoracotomy only. PAI was performed both at the beginning and before the end of the surgical procedure. The PAI results were compared between the two groups to analyze the relationship between myocardial PAI signal changes and oxygen metabolism levels.</p><p><strong>Results: </strong>Following coronary ligation, the experimental group exhibited significant ST-segment elevation on electrocardiography, whereas no notable changes were observed in controls. PAI demonstrated: baseline myocardial oxygen saturation ( <math> <mrow><msub><mi>SmO</mi> <mn>2</mn></msub> </mrow> </math> ) ranged from 45% to 72% across all rabbits. Ligation-induced ischemia sharply reduced <math> <mrow><msub><mi>SmO</mi> <mn>2</mn></msub> </mrow> </math> to 1% to 19% in experimental animals. Control animals maintained stable <math> <mrow><msub><mi>SmO</mi> <mn>2</mn></msub> </mrow> </math> levels throughout the procedure. Histopathological examination confirmed extensive myocardial necrosis in the apical region of ligated rabbits, consistent with the observed functional and metabolic alterations.</p><p><strong>Conclusions: </strong>PAI can detect myocardial oxygen saturation in real-time during surgery and determine the occurrence of myocardial ischemia and changes in oxygen metabolism levels based on differences in oxygen saturation.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 10","pages":"105001"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12502845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Segmental airway adenocarcinoma-simulating phantom for endoscopic near-infrared optical coherence tomography. 用于内镜近红外光学相干断层扫描的段状气道腺癌模拟模型。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-10-01 Epub Date: 2025-10-07 DOI: 10.1117/1.JBO.30.10.105002
Eric Brace, Alicia Fung, Adrian Tanskanen, Jeanie Malone, Calum E MacAulay, Pierre M Lane
{"title":"Segmental airway adenocarcinoma-simulating phantom for endoscopic near-infrared optical coherence tomography.","authors":"Eric Brace, Alicia Fung, Adrian Tanskanen, Jeanie Malone, Calum E MacAulay, Pierre M Lane","doi":"10.1117/1.JBO.30.10.105002","DOIUrl":"10.1117/1.JBO.30.10.105002","url":null,"abstract":"<p><strong>Significance: </strong>There is an unmet need for readily accessible imaging targets to verify whether devices can discriminate lesions from healthy tissue and identify sub-surface vasculature in the small airways.</p><p><strong>Aim: </strong>Our aim is to develop a phantom that mimics human segmental airway adenocarcinoma <i>in vivo</i> for 1310 nm endoscopic optical coherence tomography (OCT) and angiography characterization.</p><p><strong>Approach: </strong>We develop phantoms using a mixture of agar, intralipid, and coconut oil cured in a 3D printed mold with embedded tubing to mimic vasculature. The parenchyma optical attenuation coefficient (OAC) is calibrated using optical transmission measurements from an agar and intralipid dilution series. Depth-resolved OAC histogram distributions, analysis of variance, and image quality are used to assess repeatability and biofidelity of these phantoms.</p><p><strong>Results: </strong>Transmission measurements show large increases in OAC when intralipid is cured with agar compared with water-intralipid dilutions. Representative phantom OACs show repeatability within 2.7% and match normal <i>in vivo</i> tissue measurements within 16%. Embedded lesion phantoms achieve imaging characteristics of <i>in vivo</i> adenocarcinoma. Fluid flow within embedded tubing is visualized with Doppler OCT.</p><p><strong>Conclusions: </strong>The segmental airway phantoms demonstrate <i>in vivo</i> human imaging characteristics, including structural and optical markers of pathological progression-providing a platform for imaging system characterization and optimization.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 10","pages":"105002"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrathin lensed fiber-based manual scanning optical coherence tomography needle probe for the detection of the interproximal caries. 基于超薄透镜光纤的手工扫描光学相干断层扫描针探头用于近端间龋的检测。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-10-01 Epub Date: 2025-10-14 DOI: 10.1117/1.JBO.30.10.106001
Tong Wu, Yu Zhao, Jie He, YuFei Shan, Hong Shen, Youwen Liu, YaoYao Shi, XiaoRong Gu, YuanGang Lu, Jiming Wang, ChongJun He
{"title":"Ultrathin lensed fiber-based manual scanning optical coherence tomography needle probe for the detection of the interproximal caries.","authors":"Tong Wu, Yu Zhao, Jie He, YuFei Shan, Hong Shen, Youwen Liu, YaoYao Shi, XiaoRong Gu, YuanGang Lu, Jiming Wang, ChongJun He","doi":"10.1117/1.JBO.30.10.106001","DOIUrl":"10.1117/1.JBO.30.10.106001","url":null,"abstract":"<p><strong>Significance: </strong>Interproximal caries detection is critical for effective dental treatment. We report an ultrathin lensed fiber-based manual scanning optical coherence tomography (OCT) needle probe to enables the direct imaging of the interproximal caries between two adjacent teeth.</p><p><strong>Aim: </strong>We aim to design and fabricate the ultrathin lensed fiber-based manual scanning OCT needle probe, and validate the performance of the proposed probe by applying it to the imaging of the phantom sample, the human skin tissue and the interproximal caries between two adjacent teeth.</p><p><strong>Approach: </strong>A homemade lensed fiber is packaged into a 21-gauge hypodermic needle to create a high-flexibility, ultrathin probe. A decorrelation algorithm is employed for image reconstruction based on manual scanning. The performances of the developed needle probe are experimentally measured. The probe is incorporated in a swept-source OCT system to image the phantom sample, the human skin tissue, and the interproximal caries between two adjacent teeth.</p><p><strong>Results: </strong>The working distance and focused spot diameter of the developed probe are measured to be 1.22 mm and <math><mrow><mn>18.78</mn> <mtext>  </mtext> <mi>μ</mi> <mi>m</mi></mrow> </math> , respectively. The correctly reconstructed OCT images of the phantom, skin tissue, and the tooth tissue demonstrate the performance of the developed ultrathin lensed fiber-based manual scanning OCT needle probe. The distinct structural difference between the healthy and abnormal teeth tissue validates the efficacy of the proposed method.</p><p><strong>Conclusion: </strong>We propose an ultrathin lensed fiber-based manual scanning OCT needle probe potentially useful for the interproximal caries detection. The design, fabrication, and performances of the developed needle probe are demonstrated. We address a critical issue in the caries diagnostics and offer a promising tool for the future clinical applications.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 10","pages":"106001"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12519090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145300838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advanced automated classification and segmentation of leukemic cells using simulated optical scanning holography and active contour methods. 利用模拟光学扫描全息和主动轮廓方法对白血病细胞进行高级自动分类和分割。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-09-01 Epub Date: 2025-09-27 DOI: 10.1117/1.JBO.30.9.096005
Abdennacer El-Ouarzadi, Abdelaziz Essadike, Younes Achaoui, Abdenbi Bouzid
{"title":"Advanced automated classification and segmentation of leukemic cells using simulated optical scanning holography and active contour methods.","authors":"Abdennacer El-Ouarzadi, Abdelaziz Essadike, Younes Achaoui, Abdenbi Bouzid","doi":"10.1117/1.JBO.30.9.096005","DOIUrl":"10.1117/1.JBO.30.9.096005","url":null,"abstract":"<p><strong>Significance: </strong>Leukemia, a complex hematological cancer, poses significant diagnostic challenges due to the heterogeneity of leukemic cells, inter-observer variability, and lack of standardized analysis methodology. Accurate and rapid cell classification is essential to improve clinical management, optimize treatment, and reduce diagnostic errors.</p><p><strong>Aim: </strong>We propose an innovative approach combining optical scanning holography (OSH) and active contour (AC) models to automate the classification and segmentation of leukemic cells with increased accuracy.</p><p><strong>Approach: </strong>OSH is used to capture the phase current of leukocytes, providing a cost-effective, noninvasive, and simplified alternative to conventional techniques. AC models are used to improve cell segmentation. Analysis of the maximum amplitude values of the phase current allows rapid and fully automated classification.</p><p><strong>Results: </strong>The proposed approach shows a significant improvement in terms of reliability, speed, and reproducibility compared with existing methods. The integration of OSH and AC enables robust segmentation and efficient classification of leukemic cells.</p><p><strong>Conclusion: </strong>This method provides a reliable, rapid, and systematic solution for the accurate diagnosis of leukemia, enabling optimized therapeutic management.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 9","pages":"096005"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lightweight and precise cell classification based on holographic tomography-derived refractive index point cloud. 基于全息层析折射率点云的轻量化精确细胞分类。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-09-01 Epub Date: 2025-09-02 DOI: 10.1117/1.JBO.30.9.096501
Haoyuan Wang, Difeng Wu, Miao Zheng, Zuoshuai Zhang, Weina Zhang, Jianglei Di, Liyun Zhong
{"title":"Lightweight and precise cell classification based on holographic tomography-derived refractive index point cloud.","authors":"Haoyuan Wang, Difeng Wu, Miao Zheng, Zuoshuai Zhang, Weina Zhang, Jianglei Di, Liyun Zhong","doi":"10.1117/1.JBO.30.9.096501","DOIUrl":"10.1117/1.JBO.30.9.096501","url":null,"abstract":"<p><strong>Significance: </strong>Accurate cell classification is essential in disease diagnosis and drug screening. Three-dimensional (3D) voxel models derived from holographic tomography effectively capture the internal structural features of cells, enhancing classification accuracy. However, their high dimensionality leads to significant increases in data volume, computational complexity, processing time, and hardware costs, which limit their practical applicability.</p><p><strong>Aim: </strong>We aim to develop an efficient and accurate cell classification method using 3D refractive index (RI) point cloud data obtained from holographic tomography, focusing on reducing computational complexity without sacrificing classification performance.</p><p><strong>Approach: </strong>We transformed 3D RI voxel data into point cloud representations using segmented equilibrium sampling to substantially decrease data volume while retaining crucial structural features. A deep learning model, named RI-PointNet++, was then specifically designed for RI point cloud data to enhance feature extraction and enable precise cell classification.</p><p><strong>Results: </strong>In experiments classifying the viability of HeLa cells, the proposed method achieved a classification accuracy of 93.5%, significantly outperforming conventional two-dimensional models (87.0%). Furthermore, compared with traditional 3D voxel-based models, our method reduced computational complexity by over 99%, with floating-point operations of only 1.49 G, thus enabling efficient performance even on central processing unit (CPU) hardware.</p><p><strong>Conclusions: </strong>Our proposed method provides an innovative, lightweight solution for 3D cell classification, highlighting the considerable potential of point cloud-based approaches in biomedical research applications.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 9","pages":"096501"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12404102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mathematical modeling and analysis for tissue curvature correction in near-infrared spectroscopy imaging. 近红外光谱成像中组织曲率校正的数学建模与分析。
IF 2.9 3区 医学
Journal of Biomedical Optics Pub Date : 2025-09-01 Epub Date: 2025-09-19 DOI: 10.1117/1.JBO.30.9.096002
Himaddri Shakhar Roy, Daniela Leizaola, Charles Policard, Anuradha Godavarty
{"title":"Mathematical modeling and analysis for tissue curvature correction in near-infrared spectroscopy imaging.","authors":"Himaddri Shakhar Roy, Daniela Leizaola, Charles Policard, Anuradha Godavarty","doi":"10.1117/1.JBO.30.9.096002","DOIUrl":"10.1117/1.JBO.30.9.096002","url":null,"abstract":"<p><strong>Significance: </strong>Near-infrared spectroscopy (NIRS) imaging modalities are used to provide noncontact measurements of tissue oxygenation in diabetic foot ulcers. However, the curved surface of the diabetic foot introduces inaccurate tissue oxygenation measurement. The changes in spatial NIRS optical measurements may result from variations in the underlying physiology or from the curvature of the tissue surface. Therefore, the effect of tissue curvature must be accounted for to ensure the accurate measurement of tissue oxygenation (or hemoglobin parameters) in clinical applications.</p><p><strong>Aim: </strong>Our aim is to develop and validate mathematical curvature correction models to account for the effects of tissue curvature on diffuse reflectance (DR) in NIRS imaging and assess their effect on the hemoglobin parameters as well.</p><p><strong>Approach: </strong>Monte-Carlo-based light propagation simulations were performed to develop correction models and applied to three-layered curved geometries in MCMatlab. Four curvature correction models based on height and/or angle were developed via Monte Carlo simulation studies. All the correction models were applied to the simulated DR signals obtained from various curved geometries (concave, convex, and wound-mimicking) using Gaussian light sources at 690 and 830 nm. The effect of correction models on DR signals and hemoglobin parameters was determined.</p><p><strong>Results: </strong>Simulation results showed that a concave curved surface did not require correction, whereas convex and wound-mimicking geometries showed a reduced median error upon using an empirical height/angle correction model. In addition, the correction model also reduced the median error significantly for the oxygen-saturation-based hemoglobin parameter in both the convex and wound-mimicking geometries.</p><p><strong>Conclusions: </strong>The developed mathematical model effectively corrected tissue curvature effects in NIRS DR signals and hemoglobin parameters for wound-mimicking irregular geometry. Ongoing work focuses on experimental validation of these correction models on curved phantoms, prior to <i>in vivo</i> imaging studies.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 9","pages":"096002"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信