Evaluating blue light impact on reconstructed human epidermis using laser speckle imaging.

Significance: Blue light exposure is ubiquitous in modern life, raising concerns about its potential impact on skin health.

Aim: We aim to explore the effects of blue light on the reconstructed human epidermis (RHE) using the speckle analysis technique.

Approach: RHE samples were irradiated with controlled doses of blue light (415 and 455 nm) at defined stages of their maturation. Following irradiation, speckle analysis was performed to assess the impact of blue light on the skin barrier.

Results: Our results demonstrate that blue light irradiation significantly alters the scattering properties of RHE. Both wavelengths induced changes in the degree of linear polarization and speckle grain size, indicating disruptions in the skin barrier's structure and organization. The effects were found to be wavelength-dependent, with 455 nm irradiation showing more pronounced changes.

Conclusions: Speckle imaging allowed detection of changes in the scattering properties of RHE. Findings suggest that blue light exposure can influence skin barrier function and may have implications for skin health and related conditions.