Journal of Biomedical Optics最新文献

{"title":"Depth-resolved imaging in turbid media via Mueller matrix polarimetry.","authors":"Xinxian Zhang, Jiahao Fan, Jiawei Song, Nan Zeng, Honghui He, Valery V Tuchin, Hui Ma","doi":"10.1117/1.JBO.30.5.056009","DOIUrl":"https://doi.org/10.1117/1.JBO.30.5.056009","url":null,"abstract":"<p><strong>Significance: </strong>Polarimetry offers advantages such as high information dimensionality and sensitivity to microstructures. Determining the depth of the tissue is essential for clinical diagnosis and treatment, such as lesion localization, removal, and drug delivery. However, relying solely on polarization techniques for tissue depth measurement remains a subject for further investigation.</p><p><strong>Aim: </strong>We aim to investigate the tissue depth measurement in turbid media using Mueller matrix polarimetry, with a focus on fibrous tissues.</p><p><strong>Approach: </strong>Tissue phantoms are constructed to quantitatively simulate fibrosis at specific depth. By analyzing Mueller matrix measurements across depth gradients, correlations between polarization basic parameters (PBPs) and tissue depth are established using supervised machine learning algorithms.</p><p><strong>Results: </strong>We introduce an approach by combining degree of polarization (DOP)-sensitive PBPs with anisotropy-sensitive PBPs to develop depth-sensitive polarization feature parameters (DSPFPs). The DSPFPs exhibit enhanced sensitivity to depth in shallow layers while preserving accuracy in deeper layers. The effectiveness and robustness of the proposed method are validated through 2D depth-resolved imaging of tissue phantoms.</p><p><strong>Conclusions: </strong>We preliminarily explore the feasibility of depth measurement using Mueller matrix polarimetry, establishing a method for tissue depth assessment while also expanding the applications of polarimetry.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 5","pages":"056009"},"PeriodicalIF":3.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward handheld optically guided biopsy combining diffuse reflectance spectroscopy and autofluorescence.","authors":"Lotte M de Roode, Simon T Sørensen, Stefan D van der Stel, Lisanne L de Boer, Yineng Wang, Huihui Lu, Stefan Andersson-Engels, Theo J M Ruers, Ray Burke","doi":"10.1117/1.JBO.30.5.057001","DOIUrl":"https://doi.org/10.1117/1.JBO.30.5.057001","url":null,"abstract":"<p><strong>Significance: </strong>We aim to validate the technologies essential for a handheld, optically guided biopsy device designed to enhance the diagnostic yield and accuracy of percutaneous liver biopsy procedures.</p><p><strong>Aim: </strong>We aim to combine diffuse reflectance spectroscopy (DRS) and autofluorescence (AF) spectroscopy in a fiber-optic needle probe using mini spectrometers for the classification of tumor and healthy tissues.</p><p><strong>Approach: </strong>A fiber-optic needle probe combining DRS and AF spectroscopy, incorporating mini spectrometers to facilitate future integration into a biopsy actuator, was designed and built. This custom probe was used to measure healthy liver tissues and colorectal metastases in excised liver segments. A linear discriminant analysis was applied to the DRS and AF data to distinguish tumors from healthy tissues.</p><p><strong>Results: </strong>The miniaturized combined DRS and AF spectroscopy system could accurately distinguish tumors from healthy liver with a sensitivity of 95% and a specificity of 96% from a sample size of <math><mrow><mi>N</mi> <mo>=</mo> <mn>10</mn></mrow> </math> patients and 52 measurements.</p><p><strong>Conclusions: </strong>We demonstrate the feasibility of miniaturizing a combined DRS and AF spectroscopy system for the classification of tumor and healthy tissues. This validation supports the feasibility and further development of a handheld, optically guided biopsy device.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 5","pages":"057001"},"PeriodicalIF":3.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12054881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Elucidating the effect of tumor and background region-of-interest selection on the performance metrics used to assess fluorescence imaging (Erratum).","authors":"Augustino V Scorzo, Caleb Y Kwon, Rendall R Strawbridge, P Jack Hoopes, David W Roberts, Scott C Davis","doi":"10.1117/1.JBO.30.5.059801","DOIUrl":"https://doi.org/10.1117/1.JBO.30.5.059801","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1117/1.JBO.30.4.046004.].</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 5","pages":"059801"},"PeriodicalIF":3.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep structural brain imaging via computational three-photon microscopy.","authors":"Lingmei Chen, Mubin He, Lu Yang, Lingxi Zhou, Shuhao Qian, Chuncheng Wang, Rushan Jiang, Zhihua Ding, Jun Qian, Zhiyi Liu","doi":"10.1117/1.JBO.30.4.046002","DOIUrl":"10.1117/1.JBO.30.4.046002","url":null,"abstract":"<p><strong>Significance: </strong>High-resolution optical imaging at significant depths is challenging due to scattering, which impairs image quality in living matter with complex structures. We address the need for improved imaging techniques in deep tissues.</p><p><strong>Aim: </strong>We aim to develop a computational deep three-photon microscopy (3PM) method that enhances image quality without compromising acquisition speed, increasing excitation power, or adding extra optical components.</p><p><strong>Approach: </strong>We introduce a method called low-rank diffusion model (LRDM)-3PM, which utilizes customized aggregation-induced emission nanoprobes and self-supervised deep learning. This approach leverages superficial information from three-dimensional (3D) images to compensate for scattering and structured noise from the imaging system.</p><p><strong>Results: </strong>LRDM-3PM achieves a remarkable signal-to-background ratio above 100 even at depths of 1.5 mm, enabling the imaging of the hippocampus in live mouse brains. It integrates with a multiparametric analysis platform for resolving morpho-structural features of brain vasculature in a completely 3D manner, accurately recognizing distinct brain regions.</p><p><strong>Conclusions: </strong>LRDM-3PM demonstrates the potential for minimally invasive <i>in vivo</i> imaging and analysis, offering a significant advancement in the field of deep tissue imaging by maintaining high-resolution quality at unprecedented depths.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 4","pages":"046002"},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-channel pulse-dye densitometry can enable correction of fluorescent targeted and control agent plasma input function differences for quantitative paired-agent molecular imaging: a simulation study.","authors":"Cody C Rounds, Yichen Feng, Sanjana Pannem, Jovan Brankov, Kimberly S Samkoe, Kenneth M Tichauer","doi":"10.1117/1.JBO.30.4.046001","DOIUrl":"10.1117/1.JBO.30.4.046001","url":null,"abstract":"<p><strong>Significance: </strong>Paired-agent fluorescent molecular imaging approaches involve co-administration of a control (untargeted) imaging agent with a molecularly targeted agent to account for non-specific effects and quantify binding potential (BP)-a parameter proportional to the concentration of the targeted biomolecule. Accurate BP estimation often requires correction for differences in targeted and control agent plasma input functions (PIFs).</p><p><strong>Aim: </strong>We provide a simulation-based evaluation of whether dual-channel pulse dye densitometry (PDD) can be used to measure the PIFs of co-administered targeted and control imaging agents, to enable accurate BP estimation.</p><p><strong>Approach: </strong>Monte-Carlo simulations of light propagation were carried out using the anatomy and optical properties of a finger, as well as experimentally measured PIFs of co-administered anti-epidermal growth factor receptor fluorescent affibody, ABY-029, and IRDye 680LT, a control imaging agent from past mouse experiments. The accuracy of PIF shape estimation from PDD and PIF difference correction was evaluated by assessing BP estimation accuracy in a simulated \"tumor\" tissue.</p><p><strong>Results: </strong>\"Tumor\" BP measurements using deconvolution correction with noise-free PIFs versus PDD-measured PIFs were compared. The relative error in PDD PIF deconvolution BP estimation was <math><mrow><mn>2</mn> <mo>±</mo> <mn>1</mn> <mo>%</mo></mrow> </math> . No statistical difference was found between the estimated BP via deconvolution correction with true PIFs and the estimated BP via the reconstructed PIFs using the proposed PAF-PDD methodology.</p><p><strong>Conclusions: </strong>These results highlight the potential for developing a PDD instrument that can directly measure targeted and control agent PIFs and be used to correct for any PIF differences between agents for more quantitative estimates of BP in paired-agent imaging studies.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 4","pages":"046001"},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Choice of numerical implementation of spatial contrast calculation impacts microcirculation quantitation in laser speckle contrast imaging.","authors":"Marc Chammas, Frédéric Pain","doi":"10.1117/1.JBO.30.4.046006","DOIUrl":"https://doi.org/10.1117/1.JBO.30.4.046006","url":null,"abstract":"<p><strong>Significance: </strong>Laser speckle contrast imaging (LSCI) allows noninvasive imaging of microcirculation. Its scope of clinical applications is growing, yet the literature lacks a comparison of the accuracy of methods used to compute the spatial contrast <math> <mrow><msub><mi>K</mi> <mi>s</mi></msub> </mrow> </math> from which the blood flow index is derived.</p><p><strong>Aim: </strong>We aim to evaluate the impact on flow quantitation of different computational approaches used to derive <math> <mrow><msub><mi>K</mi> <mi>s</mi></msub> </mrow> </math> .</p><p><strong>Approach: </strong>We compare numerical calculation of <math> <mrow><msub><mi>K</mi> <mi>s</mi></msub> </mrow> </math> in Python and ImageJ applied to noise-free simulated data and to experimental data acquired <i>in vivo</i> in anesthetized mice. The estimation of the decorrelation time <math> <mrow><msub><mi>τ</mi> <mi>c</mi></msub> </mrow> </math> , inversely proportional to the blood flow index, is carried out following two approaches: LSCI asymptotic estimation and fitting the multiple exposure speckle imaging (MESI) model to <math> <mrow><msub><mi>K</mi> <mi>s</mi></msub> <mo>(</mo> <mi>T</mi> <mo>)</mo></mrow> </math> .</p><p><strong>Results: </strong>For simulation data, we found variations of up to 58% for the blood flow index in the LSCI approach. Nonlinear fitting of the MESI model was less affected with discrepancies of only a few percent. Considering experimental data, the LSCI approximation led to <math> <mrow><msub><mi>K</mi> <mi>s</mi></msub> </mrow> </math> with relative differences (up to 35%) depending on the calculation methods. The noise and limited exposure time strongly limited the accuracy of the LSCI asymptotic estimation. Adjustment of the MESI model to the data led to consistent values of <math> <mrow><msub><mi>τ</mi> <mi>c</mi></msub> </mrow> </math> in the 0.05 to 1 ms range with significant variations depending on the method used to calculate <math> <mrow><msub><mi>K</mi> <mi>s</mi></msub> </mrow> </math> .</p><p><strong>Conclusions: </strong>Numerical methods used to calculate <math> <mrow><msub><mi>K</mi> <mi>s</mi></msub> </mrow> </math> should be precisely acknowledged and validated against direct calculation to ensure accuracy. <i>Uniform</i> filter approach leads to accurate <math> <mrow><msub><mi>K</mi> <mi>s</mi></msub> </mrow> </math> values and is 100 times more computationally efficient than the <math><mrow><mi>D</mi> <mi>i</mi> <mi>r</mi> <mi>e</mi> <mi>c</mi> <mi>t</mi></mrow> </math> calculation. Other investigated methods lead to various levels of errors in flow index estimation using LSCI. Errors are minimized using larger kernels. MESI derivation of <math> <mrow><msub><mi>τ</mi> <mi>c</mi></msub> </mrow> </math> is not immune but less affected by such methodological biases.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 4","pages":"046006"},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency domain broadband short-wave infrared spectroscopy for measurement of tissue optical properties from 685 to 1300 nm.","authors":"Diana Suciu, Thao Pham, Lina Lin Wei, Shripreetika Guruprasad, Darren Roblyer","doi":"10.1117/1.JBO.30.4.045001","DOIUrl":"https://doi.org/10.1117/1.JBO.30.4.045001","url":null,"abstract":"<p><strong>Significance: </strong>Extending frequency domain diffuse optical spectroscopy (FD-DOS) into the short-wave infrared (SWIR) region has the potential to improve measurements of key biological tissue chromophores such as water and lipids, given their higher absorption in SWIR compared with near-infrared wavelengths. Few studies have explored FD-DOS in the SWIR range.</p><p><strong>Aim: </strong>We present the first demonstration of a frequency domain broadband SWIR spectroscopy (FD-Bb-SWIRS) system to measure optical properties from 685 to 1300 nm.</p><p><strong>Approach: </strong>A custom hybrid system was developed, combining discrete frequency domain measurements from 685 to 980 nm with broadband continuous wave measurements from 900 to 1300 nm. This setup provided absolute absorption ( <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> ) spectra from 685 to 1300 nm. Validation was performed using mineral oil-based solid phantoms, deuterium oxide ( <math> <mrow><msub><mi>D</mi> <mn>2</mn></msub> <mi>O</mi></mrow> </math> ) liquid phantoms, and desiccating porcine tissue.</p><p><strong>Results: </strong>The FD-Bb-SWIRS system was sensitive to changes in <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> from varying concentrations of absorbers in solid and liquid phantoms. <i>Ex vivo</i> measurements of <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> spectra indicated differences in tissue water content across different porcine tissue samples during baseline and desiccation.</p><p><strong>Conclusions: </strong>FD-Bb-SWIRS is highly sensitive to <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> in the 685 to 1300 nm range and enables precise quantification of water in biological tissues. It represents a significant step forward in advancing SWIR-based optical spectroscopy for clinical applications.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 4","pages":"045001"},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical characteristics of human lung cancer for photodynamic therapy with measured absorption and reduced scattering coefficients.","authors":"Yu Shimojo, Yuri Morizane, Takumi Sonokawa, Jitsuo Usuda, Takahiro Nishimura","doi":"10.1117/1.JBO.30.4.048001","DOIUrl":"10.1117/1.JBO.30.4.048001","url":null,"abstract":"<p><strong>Significance: </strong>The optical characteristics of a human lung, such as the light distribution in the tissue, are crucial for evaluating the light delivery of photodynamic therapy (PDT) for peripheral lung cancer.</p><p><strong>Aim: </strong>The light distribution in the human lung is analyzed with absorption ( <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> ) and reduced scattering ( <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> ) coefficients measured <i>ex vivo</i> for normal, carbon-deposited, and tumor tissues.</p><p><strong>Approach: </strong>The <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> and <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> spectra were measured using a double-integrating-sphere optical system and inverse Monte Carlo technique. The measured values were used to perform a light distribution analysis using a Monte Carlo light transport simulation.</p><p><strong>Results: </strong>The <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> values varied between tissue types owing to the influence of carbon deposition, blood volume fraction, and oxygen saturation, whereas the <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> values showed almost no differences between tissue types. The simulation results showed that carbon deposition in the surrounding tissue and oxygen saturation variability had almost no effect on PDT light delivery to a tumor with a 10-mm-diameter sphere.</p><p><strong>Conclusions: </strong>Our analysis revealed the influence of the optical characteristics of the lung tissue on PDT light delivery. Integration of these results with the photosensitizer dose and the degree of necrosis changes will allow us to provide more clinically relevant insight in determining PDT dosimetry.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 4","pages":"048001"},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11960791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective on the use of optics in bladder cancer detection and diagnosis.","authors":"Marinka J Remmelink, Dylan J Peterson, Jakko A Nieuwenhuijzen, Ton G van Leeuwen, Joseph C Liao, Daniel M de Bruin","doi":"10.1117/1.JBO.30.4.040601","DOIUrl":"10.1117/1.JBO.30.4.040601","url":null,"abstract":"<p><strong>Significance: </strong>Bladder cancer (BC) diagnosis, management, and outcomes depend on the accurate detection of tumors via optical technologies. Accordingly, understanding the benefits and limitations of these technologies permits improvements in patient care and identifies areas for future research.</p><p><strong>Aim: </strong>We outline the current process of BC detection and diagnosis, explore the current role of optical technologies, and discuss the opportunities and challenges they present in this field.</p><p><strong>Approach: </strong>The current diagnostic pathway of BC, the use of optical technologies, and their shortcomings in this process are reviewed. From there, opportunities and challenges of optics in BC detection and diagnosis are discussed.</p><p><strong>Results: </strong>BC management is expensive due to the limitations of white light cystoscopy, the requirement for histopathological confirmation, and the need for long-term surveillance. Alternative non-optical methods lack accuracy, and available optical techniques focus only on cancer detection. Alternatives to histopathology need to provide accurate real-time results to be effective. Optical advancements offer potential benefits; however, challenges include cost-effectiveness, device complexity, required training, and tumor heterogeneity.</p><p><strong>Conclusions: </strong>Optical techniques could accelerate BC diagnosis, reduce costs, and enable alternative treatments. However, overcoming technical and practical challenges is essential for their successful integration.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 4","pages":"040601"},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LED-based multicolor extended resolution transmission fluorescence microscopy.","authors":"Huaiyuan Zhang, Yiting Hu, Xingwei Pu, Shizheng Zhang, Yi He, Kun Chen, Ziji Liu","doi":"10.1117/1.JBO.30.4.046501","DOIUrl":"10.1117/1.JBO.30.4.046501","url":null,"abstract":"<p><strong>Significance: </strong>The multiplexing capabilities of fluorescence imaging are enhanced by its exceptional molecular specificity with diverse fluorescent probes, making it a powerful tool for studying complex biological structures, organization, and functions. Recent advances in super-resolution fluorescence microscopy have further revolutionized our ability to explore biology and related fields. However, current multicolor super-resolution fluorescence imaging systems often come with high costs and bulky designs.</p><p><strong>Aim: </strong>We present a multicolor extended resolution fluorescence imaging system that uses light-emitting diode to simplify the optical path, make the design more compact, and reduce system costs.</p><p><strong>Approach: </strong>This multicolor extended resolution fluorescence imaging system is based on structured illumination, utilizing a simple diffraction unit positioned between the light source and the sample in a wide-field microscope. Notably, this design could be easily integrated into standard widefield microscopes as a convenient add-on unit, enabling extended resolution imaging.</p><p><strong>Results: </strong>Our system demonstrates concurrent extended resolved imaging of three-color microsphere beads and successfully showcases multicolor extended resolution fluorescence imaging of biological tissue samples, revealing intricate structural details.</p><p><strong>Conclusions: </strong>This system provides a structurally simple, cost-effective alternative to traditional microscopes, offering flexible multicolor extended resolution fluorescence imaging and potential applications in multimodal imaging.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 4","pages":"046501"},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}