Journal of Biomedical Optics最新文献

{"title":"Comparative analysis of intraoperative thermal and optical imaging for identification of the human primary sensory cortex.","authors":"Juliane Müller, Martin Oelschlägel, Stephan B Sobottka, Matthias Kirsch, Gerald Steiner, Edmund Koch, Christian Schnabel","doi":"10.1117/1.JBO.30.1.016002","DOIUrl":"10.1117/1.JBO.30.1.016002","url":null,"abstract":"<p><strong>Significance: </strong>The precise identification and preservation of functional brain areas during neurosurgery are crucial for optimizing surgical outcomes and minimizing postoperative deficits. Intraoperative imaging plays a vital role in this context, offering insights that guide surgeons in protecting critical cortical regions.</p><p><strong>Aim: </strong>We aim to evaluate and compare the efficacy of intraoperative thermal imaging (ITI) and intraoperative optical imaging (IOI) in detecting the primary somatosensory cortex, providing a detailed assessment of their potential integration into surgical practice.</p><p><strong>Approach: </strong>Data from nine patients undergoing tumor resection in the region of the somatosensory cortex were analyzed. Both IOI and ITI were employed simultaneously, with a specific focus on the areas identified as the primary somatosensory cortex (S1 region). The methodologies included a combination of imaging techniques during distinct phases of rest and stimulation, confirmed by electrophysiological monitoring of somatosensory evoked potentials to verify the functional areas identified by both imaging methods. The data were analyzed using a Fourier-based analytical framework to distinguish physiological signals from background noise.</p><p><strong>Results: </strong>Both ITI and IOI successfully generated reliable activity maps following median nerve stimulation. IOI showed greater consistency across various clinical scenarios, including those involving cortical tumors. Quantitative analysis revealed that IOI could more effectively differentiate genuine neuronal activity from artifacts compared with ITI, which was occasionally prone to false positives in the presence of cortical abnormalities.</p><p><strong>Conclusions: </strong>ITI and IOI produce comparable functional maps with moderate agreement in Cohen's kappa values. Their distinct physiological mechanisms suggest complementary use in specific clinical scenarios, such as cortical tumors or impaired neurovascular coupling. IOI excels in spatial resolution and mapping reliability, whereas ITI provides additional insights into metabolic changes and tissue properties, especially in pathological areas. Combined, these modalities could enhance the understanding and analysis of functional and pathological processes in complex neurosurgical cases.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 1","pages":"016002"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection properties of indium-111 and IRDye800CW for intraoperative molecular imaging use across tissue phantom models.","authors":"ReidAnn E Sever, Lauren T Rosenblum, Kayla C Stanley, Angel G Cortez, Dominic M Menendez, Bhuvitha Chagantipati, Jessie R Nedrow, W Barry Edwards, Marcus M Malek, Gary Kohanbash","doi":"10.1117/1.JBO.30.S1.S13705","DOIUrl":"10.1117/1.JBO.30.S1.S13705","url":null,"abstract":"<p><strong>Significance: </strong>Intraoperative molecular imaging (IMI) enables the detection and visualization of cancer tissue using targeted radioactive or fluorescent tracers. While IMI research has rapidly expanded, including the recent Food and Drug Administration approval of a targeted fluorophore, the limits of detection have not been well-defined.</p><p><strong>Aim: </strong>The ability of widely available handheld intraoperative tools (Neoprobe and SPY-PHI) to measure gamma decay and fluorescence intensity from IMI tracers was assessed while varying characteristics of both the signal source and the intervening tissue or gelatin phantoms.</p><p><strong>Approach: </strong>Gamma decay signal and fluorescence from tracer-bearing tumors (TBTs) and modifiable tumor-like inclusions (TLIs) were measured through increasing thicknesses of porcine tissue and gelatin in custom 3D-printed molds. TBTs buried beneath porcine tissue were used to simulate IMI-guided tumor resection.</p><p><strong>Results: </strong>Gamma decay from TBTs and TLIs was detected through significantly thicker tissue and gelatin than fluorescence, with at least 5% of the maximum signal observed through up to 5 and 0.5 cm, respectively, depending on the overlying tissue type or gelatin.</p><p><strong>Conclusions: </strong>We developed novel systems that can be fine-tuned to simulate variable tumor characteristics and tissue environments. These were used to evaluate the detection of fluorescent and gamma signals from IMI tracers and simulate IMI surgery.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13705"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic comparison of fluorescence imaging in the near-infrared and shortwave-infrared spectral range using clinical tumor samples containing cetuximab-IRDye800CW.","authors":"Bas Keizers, Thomas S Nijboer, Christa A M van der Fels, Marius C van den Heuvel, Gooitzen M van Dam, Schelto Kruijff, Igle Jan de Jong, Max J H Witjes, Floris J Voskuil, Dimitris Gorpas, Wesley R Browne, Pieter J van der Zaag","doi":"10.1117/1.JBO.30.S1.S13708","DOIUrl":"10.1117/1.JBO.30.S1.S13708","url":null,"abstract":"<p><strong>Significance: </strong>Shortwave-infrared (SWIR) imaging is reported to yield better contrast in fluorescence-guided surgery than near-infrared (NIR) imaging, due to a reduction in scattering. This benefit of SWIR was shown in animal studies, however not yet in clinical studies with patient samples.</p><p><strong>Aim: </strong>We investigate the potential benefit of SWIR to NIR imaging in clinical samples containing cetuximab-IRDye800CW in fluorescence-guided surgery.</p><p><strong>Approach: </strong>The potential of the epidermal growth factor-targeted NIR dye cetuximab-IRDye800CW in the shortwave range was examined by recording the absorption and emission spectrum. An <i>ex vivo</i> comparison of NIR and SWIR images using clinical tumor samples of patients with penile squamous cell carcinoma (PSCC) and head and neck squamous cell carcinoma (HNSCC) containing cetuximab-IRDye800CW was performed. The comparison was based on the tumor-to-background ratio and an adapted contrast-to-noise ratio (aCNR) using the standard of care pathology tissue assessment as the golden standard.</p><p><strong>Results: </strong>Based on the emission spectrum, cetuximab-IRDye800CW can be detected in the SWIR range. In clinical PSCC samples, overall SWIR imaging was found to perform similarly to NIR imaging (NIR imaging is better than SWIR in the 2/7 criteria examined, and SWIR is better than NIR in the 3/7 criteria). However, when inspecting HNSCC data, NIR is better than SWIR in nearly all (5/7) examined criteria. This difference seems to originate from background autofluorescence overwhelming the off-peak SWIR fluorescence signal in HNSCC tissue.</p><p><strong>Conclusion: </strong>SWIR imaging using the targeted tracer cetuximab-IRDye800CW currently does not provide additional benefit over NIR imaging in <i>ex vivo</i> clinical samples. Background fluorescence in the SWIR region, resulting in a higher background signal, limits SWIR imaging in HNSCC samples. However, SWIR shows potential in increasing the contrast of tumor borders in PSCC samples, as shown by a higher aCNR over a line.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13708"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-color fluorescence-guided surgery for head and neck cancer resections.","authors":"Dani A Szafran, Nourhan A Shams, Antonio Montaño, Syed Zaki Husain Rizvi, Adam W G Alani, Kimberley S Samkoe, Lei G Wang, Summer L Gibbs","doi":"10.1117/1.JBO.30.S1.S13707","DOIUrl":"https://doi.org/10.1117/1.JBO.30.S1.S13707","url":null,"abstract":"<p><strong>Significance: </strong>Head and neck squamous cell carcinoma (HNSCC) has the sixth highest incidence worldwide, with <math><mrow><mo>></mo> <mn>650,000</mn></mrow> </math> cases annually. Surgery is the primary treatment option for HNSCC, during which surgeons balance two main goals: (1) complete cancer resection and (2) preservation of normal tissues to ensure post-surgical quality of life. Unfortunately, these goals are not synergistic, where complete cancer resection is often limited by efforts to preserve normal tissues, particularly nerves, and reduce life-altering comorbidities.</p><p><strong>Aim: </strong>Currently, no clinically validated technology exists to enhance intraoperative cancer and nerve recognition. Fluorescence-guided surgery (FGS) has successfully integrated into clinical medicine, providing surgeons with real-time visualization of important tissues and complex anatomy, where FGS imaging systems operate almost exclusively in the near-infrared (NIR, 650 to 900 nm). Notably, this spectral range permits the detection of two NIR imaging channels for spectrally distinct detection.</p><p><strong>Approach: </strong>Herein, we evaluated the utility of spectrally distinct NIR nerve- and tumor-specific fluorophores for two-color FGS to guide HNSCC surgery. Using a human HNSCC xenograft murine model, we demonstrated that facial nerves and tumors could be readily differentiated using these nerve- and tumor-specific NIR fluorophores.</p><p><strong>Results: </strong>The selected nerve-specific fluorophore showed no significant difference in nerve specificity and off-target tissue fluorescence in the presence of xenograft head and neck tumors. Co-administration of two NIR fluorophores demonstrated successful tissue-specific labeling of nerves and tumors in spectrally distinct NIR imaging channels.</p><p><strong>Conclusions: </strong>We demonstrate a comprehensive FGS tool for cancer resection and nerve sparing during HNSCC procedures for future clinical translation.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13707"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective on the use of fluorescence molecular imaging for peripheral and deep <i>en face</i> margin assessment.","authors":"Hang M Nguyen, Veronica C Torres, Joshua Levy, Eunice Y Chen, Matthew LeBoeuf, Kimberley S Samkoe","doi":"10.1117/1.JBO.30.S1.S13711","DOIUrl":"https://doi.org/10.1117/1.JBO.30.S1.S13711","url":null,"abstract":"<p><strong>Significance: </strong>Current standard practice for margin assessment in solid tumor resection often leads to suboptimal results due to the inability to assess margins completely in a time-efficient manner. On the other hand, for small skin cancers, peripheral and deep <i>en face</i> margin assessment (PDEMA) offers 100% assessment of margins while sparing the utmost amount of normal surrounding tissues. Nonetheless, PDEMA is limited in its use owing to its lengthy tissue processing and imaging time as well as its requirement for high-quality frozen sections and real-time histologic analysis.</p><p><strong>Aim: </strong>We aim to explore fluorescence molecular imaging (FMI) as a tool for resolving obstacles and integrating PDEMA into the surgeon-to-pathologist workflow for large solid tumors.</p><p><strong>Approach: </strong>A review of recent pre-clinical and clinical studies using FMI to assess surgical margins was conducted to highlight promising fluorescence imaging technologies utilized in the surgical suite and laboratory.</p><p><strong>Results: </strong>FMI techniques that provide macroscopic resolution are efficient in time and have a notable ability to identify true negative tissue yet have limited capability in identifying true positive tissues. Moreover, meso- and microscopic FMI methods require additional time to attain a higher resolution but deliver an enhanced sensitivity in detecting true positive tissues. In both cases, experts are still required to learn to interpret the FMI signals, which prohibits a seamless clinical integration.</p><p><strong>Conclusions: </strong>Our proposed margin assessment platform (MAP) incorporates both macroscopic and, meso- or microscopic imaging with post-processing and machine learning for interpretation, to enable the application of PDEMA into solid tumor surgery. MAP leverages the advantages of each technique and thoroughly tackles the limitations of time and expertise to optimize the efficiency and accuracy of margin assessment and ultimately improve clinical outcomes.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13711"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Publisher's Note: Optical clearing with tartrazine enables deep transscleral imaging with optical coherence tomography.","authors":"Amit Narawane, Robert Trout, Christian Viehland, Anthony N Kuo, Lejla Vajzovic, Al-Hafeez Dhalla, Cynthia A Toth","doi":"10.1117/1.JBO.29.12.129801","DOIUrl":"https://doi.org/10.1117/1.JBO.29.12.129801","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1117/1.JBO.29.12.120501.].</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 12","pages":"129801"},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism.","authors":"Anna Theodossiou, Jocelyn Martinez, Alex J Walsh","doi":"10.1117/1.JBO.29.12.126501","DOIUrl":"10.1117/1.JBO.29.12.126501","url":null,"abstract":"<p><strong>Significance: </strong>Cellular metabolic dynamics can occur within milliseconds, yet there are no optimal tools to spatially and temporally capture these events. Autofluorescence imaging can provide metabolic information on the cellular level due to the intrinsic fluorescence of reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] and flavin adenine dinucleotide (FAD).</p><p><strong>Aim: </strong>Our goal is to build and evaluate a widefield microscope optimized for rapid autofluorescence imaging of metabolic changes in cells.</p><p><strong>Approach: </strong>A widefield, fluorescence microscope was assembled from an inverted microscope base, an light-emitting diode (LED) for excitation, and an image splitter for simultaneous but separate imaging of two bandwidths of emission (451/106 and 560/94 nm) on a single scientific complementary metal-oxide-semiconductor (sCMOS) camera. MCF-7 cells and primary murine hippocampal neurons were metabolically perturbed using cyanide and imaged to optimize illumination and camera exposure. To capture a rapid change in metabolism, MCF-7 cells were starved for 1 h and imaged while reintroduced to glucose.</p><p><strong>Results: </strong>Significant differences in the optical redox ratio (ORR) and intensity of NAD(P)H divided by the summed intensities of NAD(P)H and FAD were quantified for cyanide-treated neurons and MCF-7 cells at illumination powers above 0.30 mW and camera exposures as low as 5 ms; however, low illumination and camera exposures hindered the ability to identify subcellular features. Minimal photobleaching was quantified for 30 s of continuous imaging for illuminations at 4.14 mW and below. Using the optimized illumination power of 4.14 mW and an exposure of 10 ms, continuous autofluorescence imaging of starved MCF-7 cells demonstrated a rapid, yet heterogeneous, increase in the ORR of cells upon exposure to glucose.</p><p><strong>Conclusions: </strong>Ultimately, this widefield autofluorescence imaging system allowed for dynamic imaging and quantification of cellular metabolism at 99.6 Hz.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 12","pages":"126501"},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoacoustic and fluorescence dual-modality imaging of cerebral biomarkers in Alzheimer's disease rodent model.","authors":"Tianqu Zhai, Wei Zhang, Chenshuo Ma, Yanhui Ma, Yannis Mantas Paulus, Enming Joseph Su, Geoffrey Murphy, Daniel A Lawrence, Xueding Wang","doi":"10.1117/1.JBO.29.12.126002","DOIUrl":"10.1117/1.JBO.29.12.126002","url":null,"abstract":"<p><strong>Significance: </strong>Alzheimer's disease (AD) is a predominant form of dementia that can lead to a decline in the quality of life and mortality. The understanding of the pathological changes requires monitoring of multiple cerebral biomarkers simultaneously with high resolution. Photoacoustic microscopy resolves single capillaries, allowing investigations into the most affected types of vessels. Combined with confocal fluorescence microscopy, the relationship between plaque deposition and small vessel pathology could be better understood.</p><p><strong>Aim: </strong>We aim to introduce a dual-modality imaging system combining photoacoustic microscopy (PAM) and confocal fluorescence microscopy (CFM) to provide a comprehensive view of both cerebral cortical vessels and amyloid- <math><mrow><mi>β</mi></mrow> </math> ( <math><mrow><mi>A</mi> <mi>β</mi></mrow> </math> ) plaque in AD mouse model <i>in vivo</i> and to identify the pathological changes of these two biomarkers.</p><p><strong>Approach: </strong>We developed a dual-modality imaging system to image both cerebral vessel structure and <math><mrow><mi>A</mi> <mi>β</mi></mrow> </math> plaque on groups of mice with different ages and phenotypes. Vessel imaging is enabled by PAM, whereas <math><mrow><mi>A</mi> <mi>β</mi></mrow> </math> plaque is imaged by CFM with the aid of fluorescent dye.</p><p><strong>Results: </strong>The small vessel density in the AD group was significantly lower than in the control group, whereas the <math><mrow><mi>A</mi> <mi>β</mi></mrow> </math> plaque density in the AD group was not only higher but also increased with age.</p><p><strong>Conclusions: </strong>This dual-modality system provides a powerful platform for biomarker monitoring of AD expressing multi-dimensional pathological changes.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 12","pages":"126002"},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating hemodynamic response to treatment in patients with peripheral arterial disease using dynamic vascular optical spectroscopy.","authors":"Nisha Maheshwari, Alessandro Marone, Stephen H K Kim, Danielle R Bajakian, Andreas H Hielscher","doi":"10.1117/1.JBO.29.12.127001","DOIUrl":"10.1117/1.JBO.29.12.127001","url":null,"abstract":"<p><strong>Significance: </strong>Tracking changes in the vasculature of patients with peripheral arterial disease (PAD) may identify the need for follow-up treatment within only weeks after an initial intervention, enabling timely support and improving patient outcomes.</p><p><strong>Aim: </strong>We aim to evaluate dynamic vascular optical spectroscopy's (DVOS's) ability to accurately monitor the hemodynamics of affected arteries in patients with PAD after a surgical intervention and predict long-term clinical outcomes.</p><p><strong>Approach: </strong>A DVOS system non-invasively monitored the blood flow through 256 lower extremity arteries in 80 PAD patients immediately before, immediately after, and 3 to 4 weeks after they underwent a surgical intervention.</p><p><strong>Results: </strong>Hemodynamic changes measured by DVOS after a revascularization procedure (RP) classified patient long-term ( <math><mrow><mn>6.2</mn> <mo>±</mo> <mn>4.4</mn></mrow> </math> months) outcomes with high accuracy [81.6% for patients with ulcers ( <math><mrow><mi>n</mi> <mo>=</mo> <mn>31</mn></mrow> </math> ); 81.1% for patients without ulcers ( <math><mrow><mi>n</mi> <mo>=</mo> <mn>54</mn></mrow> </math> )] by 3 to 4 weeks after the RP, outperforming available ankle-brachial index and ultrasound measurements. In addition, DVOS parameters distinguished between patients who underwent only a catheter angiography (CA) and patients who underwent both a CA and RP ( <math><mrow><mi>P</mi> <mo><</mo> <mn>0.05</mn></mrow> </math> ).</p><p><strong>Conclusions: </strong>The DVOS system was able to classify patient long-term clinical outcomes with high accuracy within one month after an RP and distinguish among different interventions. DVOS may be a promising alternative or adjunct to existing monitoring approaches.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 12","pages":"127001"},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical clearing with tartrazine enables deep transscleral imaging with optical coherence tomography.","authors":"Amit Narawane, Robert Trout, Christian Viehland, Anthony N Kuo, Lejla Vajzovic, Al-Hafeez Dhalla, Cynthia A Toth","doi":"10.1117/1.JBO.29.12.120501","DOIUrl":"10.1117/1.JBO.29.12.120501","url":null,"abstract":"<p><strong>Significance: </strong>Imaging deep structures with optical coherence tomography (OCT) is difficult in highly scattering biological tissue, such as the sclera. There is a need to visualize the suprachoroidal space and choroid through the sclera to study suprachoroidal drug delivery.</p><p><strong>Aim: </strong>We aim to develop optical methods to image through the highly scattering sclera with a custom-built OCT system to visualize the suprachoroidal space and drug delivery within.</p><p><strong>Approach: </strong>We developed a custom handheld OCT scanner to image the anterior segment and suprachoroidal space in <i>ex vivo</i> eye models. Tartrazine (Yellow 5) solution, which has been shown to optically clear biological tissue in the visible regime, was tested as a clearing agent to optimize near infrared OCT imaging through the sclera.</p><p><strong>Results: </strong>Tartrazine dramatically increased OCT signal return from the deeper sclera and choroid and thus enabled visualization of the suprachoroidal drug delivery after transscleral injection.</p><p><strong>Conclusions: </strong>We demonstrated successful optical clearing of the thick, porcine sclera with a compact handheld OCT system to image the suprachoroidal space. We believe there is broader potential to use optical clearing with handheld OCT for a variety of previously inaccessible, highly scattering tissue samples.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 12","pages":"120501"},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11635458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}