阐明肿瘤和背景感兴趣区选择对用于评估荧光成像的性能指标的影响。

IF 2.9 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Journal of Biomedical Optics Pub Date : 2025-04-01 Epub Date: 2025-04-02 DOI:10.1117/1.JBO.30.4.046004
Augustino V Scorzo, Caleb Y Kwon, Rendall R Strawbridge, P Jack Hoopes, David W Roberts, Scott C Davis
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引用次数: 0

摘要

意义:荧光引导手术的荧光造影剂发展迅速,许多造影剂被设计用于肿瘤可视化。尽管已经努力使成像系统检测方法对这些造影剂的灵敏度标准化,但评估肿瘤造影剂性能的指南,特别是肿瘤和感兴趣背景区域(roi)的选择,在不同的研究中存在很大差异。我们研究如何系统地改变肿瘤和背景roi影响造影剂性能的常见指标。目的:阐明肿瘤和脑背景区变化对荧光造影剂性能的影响。方法:原位脑肿瘤小鼠在牺牲前40分钟给予非靶向荧光造影剂,然后使用全身荧光冷冻层析成像标本。然后,重建的三维荧光体积用于计算对比度和诊断性能指标[肿瘤与背景脑比(TBR),对比度与噪声(CNR)和接收器工作特征曲线下面积(AUC)],同时系统地改变肿瘤和正常脑roi。结果:ROI选择对用于评估荧光成像策略的指标的报告值有显著影响。对侧背景roi的使用,通常在现场使用,产生了较高的和有利的性能度量值。这些指标随着背景roi接近肿瘤边界附近区域而降低。TBR变化了5倍,CNR变化了7倍,AUC变化了10%以上,这主要取决于背景区域与肿瘤的接近程度。结论:背景ROI选择对该领域常用的绩效指标有显著影响。未来的研究应仔细选择与应用相关的roi,并包括对这些区域的清晰描述。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Elucidating the effect of tumor and background region-of-interest selection on the performance metrics used to assess fluorescence imaging.

Significance: The development of fluorescent contrast agents for fluorescence-guided surgery is rapidly growing with many agents being designed for tumor visualization. Although efforts have been made to standardize the sensitivity of imaging system detection methods for these contrast agents, guidelines to evaluate tumor contrast agent performance, especially the selection of tumor and background regions of interest (ROIs), differ widely across studies. We examine how systematically changing tumor and background ROIs affects common metrics of contrast agent performance.

Aim: We aim to elucidate the influence of changing tumor and background brain regions of interest on fluorescent contrast agent performance.

Approach: Mice with orthotopic brain tumors were administered a non-targeted fluorescent contrast agent 40 min prior to sacrifice and then imaging of the specimen using whole-body fluorescence cryotomography. The reconstructed 3D fluorescence volumes were then used to compute contrast and diagnostic performance metrics [tumor-to-background brain ratio (TBR), contrast-to-noise (CNR), and area under the receiver operating characteristic curve (AUC)] while systematically varying tumor and normal brain ROIs.

Results: ROI selection had a significant impact on the reported values of metrics used to evaluate fluorescence imaging strategies. The use of contralateral background ROIs, commonly used in the field, produced elevated and favorable performance metric values. These metrics decreased as background ROIs approached regions adjacent to the tumor boundary. TBR changed by a factor of 5, CNR by a factor of 7, and AUC by over 10%, largely depending on the proximity of the background region to the tumor.

Conclusions: Background ROI selection has a significant impact on the performance metrics commonly used in the field. Future studies should carefully select ROIs relevant to the application and include clear descriptions of these regions.

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来源期刊
CiteScore
6.40
自引率
5.70%
发文量
263
审稿时长
2 months
期刊介绍: The Journal of Biomedical Optics publishes peer-reviewed papers on the use of modern optical technology for improved health care and biomedical research.
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