Journal of Biomedical Optics最新文献

{"title":"Dynamic multispectral NIR/SWIR for <i>in vivo</i> lymphovascular architectural and functional quantification.","authors":"Christopher Hansen, Jaidip Jagtap, Abdul Parchur, Gayatri Sharma, Shayan Shafiee, Sayantan Sinha, Heather Himburg, Amit Joshi","doi":"10.1117/1.JBO.29.10.106001","DOIUrl":"https://doi.org/10.1117/1.JBO.29.10.106001","url":null,"abstract":"<p><strong>Significance: </strong>Although the lymphatic system is the second largest circulatory system in the body, there are limited techniques available for characterizing lymphatic vessel function. We report shortwave-infrared (SWIR) imaging for minimally invasive <i>in vivo</i> quantification of lymphatic circulation with superior contrast and resolution compared with near-infrared first window imaging.</p><p><strong>Aim: </strong>We aim to study the lymphatic structure and function <i>in vivo</i> via SWIR fluorescence imaging.</p><p><strong>Approach: </strong>We evaluated subsurface lymphatic circulation in healthy, adult immunocompromised salt-sensitive Sprague-Dawley rats using two fluorescence imaging modalities: near-infrared first window (NIR-I, 700 to 900 nm) and SWIR (900 to 1800 nm) imaging. We also compared two fluorescent imaging probes: indocyanine green (ICG) and silver sulfide quantum dots (QDs) as SWIR lymphatic contrast agents following intradermal footpad delivery in these rats.</p><p><strong>Results: </strong>SWIR imaging exhibits reduced scattering and autofluorescence background relative to NIR-I imaging. SWIR imaging with ICG provides 1.7 times better resolution and sensitivity than NIR-I, and SWIR imaging with QDs provides nearly two times better resolution and sensitivity with enhanced vessel distinguishability. SWIR images thus provide a more accurate estimation of <i>in vivo</i> vessel size than conventional NIR-I images.</p><p><strong>Conclusions: </strong>SWIR imaging of silver sulfide QDs into the intradermal footpad injection provides superior image resolution compared with conventional imaging techniques using NIR-I imaging with ICG dye.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 10","pages":"106001"},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11425400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mueller matrix analysis of a biologically sourced engineered tissue construct as polarimetric phantom.","authors":"Zixi Lin, Samantha Madnick, Joshua A Burrow, Jeffrey R Morgan, Kimani C Toussaint","doi":"10.1117/1.JBO.29.10.106002","DOIUrl":"https://doi.org/10.1117/1.JBO.29.10.106002","url":null,"abstract":"<p><strong>Significance: </strong>The polarimetric properties of biological tissues are often difficult to ascertain independent of their complex structural and organizational features. Conventional polarimetric tissue phantoms have well-characterized optical properties but are overly simplified. We demonstrate that an innovative, biologically sourced, engineered tissue construct better recapitulates the desired structural and polarimetric properties of native collagenous tissues, with the added benefit of potential tunability of the polarimetric response. We bridge the gap between non-biological polarimetric phantoms and native tissues.</p><p><strong>Aim: </strong>We aim to evaluate a synthesized tissue construct for its effectiveness as a phantom that mimics the polarimetric properties in typical collagenous tissues.</p><p><strong>Approach: </strong>We use a fibroblast-derived, ring-shaped engineered tissue construct as an innovative tissue phantom for polarimetric imaging. We perform polarimetry measurements and subsequent analysis using the Mueller matrix decomposition and Mueller matrix transformation methods. Scalar polarimetric parameters of the engineered tissue are analyzed at different time points for both a control group and for those treated with the transforming growth factor <math><mrow><mo>(</mo> <mi>TGF</mi> <mo>)</mo> <mtext>-</mtext> <mi>β</mi> <mn>1</mn></mrow> </math> . Second-harmonic generation (SHG) imaging and three-dimensional collagen fiber organization analysis are also applied.</p><p><strong>Results: </strong>We identify linear retardance and circular depolarization as the parameters that are most sensitive to the tissue culture time and the addition of <math><mrow><mi>TGF</mi> <mtext>-</mtext> <mi>β</mi> <mn>1</mn></mrow> </math> . Aside from a statistically significant increase over time, the behavior of linear retardance and circular depolarization indicates that the addition of <math><mrow><mi>TGF</mi> <mtext>-</mtext> <mi>β</mi> <mn>1</mn></mrow> </math> accelerates the growth of the engineered tissue, which is consistent with expectations. We also find through SHG images that collagen fiber organization becomes more aligned over time but is not susceptible to the addition of <math><mrow><mi>TGF</mi> <mtext>-</mtext> <mi>β</mi> <mn>1</mn></mrow> </math> .</p><p><strong>Conclusions: </strong>The engineered tissue construct exhibits changes in polarimetric properties, especially linear retardance and circular depolarization, over culture time and under <math><mrow><mi>TGF</mi> <mtext>-</mtext> <mi>β</mi> <mn>1</mn></mrow> </math> treatments. This tissue construct has the potential to act as a controlled modular optical phantom for polarimetric-based methods.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 10","pages":"106002"},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative estimation of optical properties in bilayer media within the subdiffusive regime using a tilted fiber-optic probe in diffuse reflectance spectroscopy, part 1: a theoretical framework for designing probe geometry.","authors":"Philippe De Tillieux, Maxime Baillot, Pierre Marquet","doi":"10.1117/1.JBO.29.10.105001","DOIUrl":"https://doi.org/10.1117/1.JBO.29.10.105001","url":null,"abstract":"<p><strong>Significance: </strong>As biological tissues are highly heterogeneous, there is a great interest in developing non-invasive optical approaches capable of characterizing them in a very localized manner. Obtaining accurate absolute values of the local optical properties from the measured reflectance requires finding a probe geometry, which allows us to solve this inverse problem robustly and reliably despite neglecting the higher-order moments of the scattering phase function.</p><p><strong>Aim: </strong>Our goal is to develop a theoretical framework for designing tilted-fiber diffuse reflectance probes that allow quantitative estimation of the optical properties corresponding to limited tissue volume (typically a few cubic millimeters).</p><p><strong>Approach: </strong>Relationships among probe geometry, sampled tissue volume, and robustness of the inverse solver to calculate optical properties from reflectance are studied using Monte Carlo simulations.</p><p><strong>Results: </strong>The analysis of the number of scattering events of the collected photons leads to the establishment of relationships among the probe geometry, the sampled tissue volume, and the validity of a subdiffusive regime for the reflectance.</p><p><strong>Conclusions: </strong>A methodology is proposed for the design of new compact probes with tilted fiber geometry that can quantitatively estimate the values of the optical coefficients in a localized manner within living biological tissues by recording diffuse reflectance spectra.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 10","pages":"105001"},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy enhancement of metabolic index-based blood glucose estimation with a screening process for low-quality data.","authors":"Tomoya Nakazawa, Keiji Morishita, Anna Ienaka, Takeo Fujii, Masaki Ito, Fumie Matsushita","doi":"10.1117/1.JBO.29.10.107001","DOIUrl":"10.1117/1.JBO.29.10.107001","url":null,"abstract":"<p><strong>Significance: </strong>Many researchers have proposed various non-invasive glucose monitoring (NIGM) approaches using wearable or portable devices. However, due to the limited capacity of detectors for such compact devices and the movement of the body during measurement, the precision of the acquired data frequently diminishes, which can cause problems during actual use in daily life. In addition, intensive smoothing is often used in post-processing to mitigate the effects of erroneous values. However, this requires a considerable amount of data and results in a delay in the response to the actual blood glucose level (BGL).</p><p><strong>Aim: </strong>Instead of just applying data smoothing in the post-process of the data acquisition, we propose an active low-quality data screening method in the pre-process. In the proposal phase of the screening process, we employ an analytical approach to examine and formulate factors that might affect the BGL estimation accuracy.</p><p><strong>Approach: </strong>A signal quality index inspired by the standard deviation concept is introduced to detect visually apparent noise on signals. Furthermore, the total estimation error in the metabolic index (MI) is calculated based on potential perturbations defined by the signal-to-noise ratio (SNR) and the uncertainty due to discrete sampling. Thereafter, the acquired data were screened by these quality indices.</p><p><strong>Results: </strong>By applying the proposed data screening process to the data obtained from a commercially available smartwatch device in the pre-process, the estimation accuracy of the MI-based BGL was improved significantly.</p><p><strong>Conclusions: </strong>Adopting the proposed screen process improves BGL estimation accuracy in the smartwatch-based prototype. Applying the proposed screen process will facilitate the integration of wearable and continuous BGL monitoring into size- and SNR-limited devices such as smartwatches and smart rings.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 10","pages":"107001"},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-resolution lensless holographic microscopy using a physics-aware deep network.","authors":"Ashwini S Galande, Vikas Thapa, Aswathy Vijay, Renu John","doi":"10.1117/1.JBO.29.10.106502","DOIUrl":"https://doi.org/10.1117/1.JBO.29.10.106502","url":null,"abstract":"<p><strong>Significance: </strong>Lensless digital inline holographic microscopy (LDIHM) is an emerging quantitative phase imaging modality that uses advanced computational methods for phase retrieval from the interference pattern. The existing end-to-end deep networks require a large training dataset with sufficient diversity to achieve high-fidelity hologram reconstruction. To mitigate this data requirement problem, physics-aware deep networks integrate the physics of holography in the loss function to reconstruct complex objects without needing prior training. However, the data fidelity term measures the data consistency with a single low-resolution hologram without any external regularization, which results in a low performance on complex biological data.</p><p><strong>Aim: </strong>We aim to mitigate the challenges with trained and physics-aware untrained deep networks separately and combine the benefits of both methods for high-resolution phase recovery from a single low-resolution hologram in LDIHM.</p><p><strong>Approach: </strong>We propose a hybrid deep framework (HDPhysNet) using a plug-and-play method that blends the benefits of trained and untrained deep models for phase recovery in LDIHM. The high-resolution phase is generated by a pre-trained high-definition generative adversarial network (HDGAN) from a single low-resolution hologram. The generated phase is then plugged into the loss function of a physics-aware untrained deep network to regulate the complex object reconstruction process.</p><p><strong>Results: </strong>Simulation results show that the SSIM of the proposed method is increased by 0.07 over the trained and 0.04 over the untrained deep networks. The average phase-SNR is elevated by 8.2 dB over trained deep models and 9.8 dB over untrained deep networks on the experimental biological cells (cervical cells and red blood cells).</p><p><strong>Conclusions: </strong>We showed improved performance of the HDPhysNet against the unknown perturbation in the imaging parameters such as the propagation distance, the wavelength of the illuminating source, and the imaging sample compared with the trained network (HDGAN). LDIHM, combined with HDPhysNet, is a portable and technology-driven microscopy best suited for point-of-care cytology applications.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 10","pages":"106502"},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous assessment of NAD(P)H and flavins with multispectral fluorescence lifetime imaging microscopy at a single excitation wavelength of 750 nm.","authors":"Boris Yakimov, Anastasia Komarova, Elena Nikonova, Artem Mozherov, Liubov Shimolina, Marina Shirmanova, Wolfgang Becker, Evgeny Shirshin, Vladislav Shcheslavskiy","doi":"10.1117/1.JBO.29.10.106501","DOIUrl":"10.1117/1.JBO.29.10.106501","url":null,"abstract":"<p><strong>Significance: </strong>Autofluorescence characteristics of the reduced nicotinamide adenine dinucleotide and oxidized flavin cofactors are important for the evaluation of the metabolic status of the cells. The approaches that involve a detailed analysis of both spectral and time characteristics of the autofluorescence signals may provide additional insights into the biochemical processes in the cells and biological tissues and facilitate the transition of spectral fluorescence lifetime imaging into clinical applications.</p><p><strong>Aim: </strong>We present the experiments on multispectral fluorescence lifetime imaging with a detailed analysis of the fluorescence decays and spectral profiles of the reduced nicotinamide adenine dinucleotide and oxidized flavin under a single excitation wavelength aimed at understanding whether the use of multispectral detection is helpful for metabolic imaging of cancer cells.</p><p><strong>Approach: </strong>We use two-photon spectral fluorescence lifetime imaging microscopy. Starting from model solutions, we switched to cell cultures treated by metabolic inhibitors and then studied the metabolism of cells within tumor spheroids.</p><p><strong>Results: </strong>The use of a multispectral detector in combination with an excitation at a single wavelength of 750 nm allows the identification of fluorescence signals from three components: free and bound NAD(P)H, and flavins based on the global fitting procedure. Multispectral data make it possible to assess not only the lifetime but also the spectral shifts of emission of flavins caused by chemical perturbations. Altogether, the informative parameters of the developed approach are the ratio of free and bound NAD(P)H amplitudes, the decay time of bound NAD(P)H, the amplitude of flavin fluorescence signal, the fluorescence decay time of flavins, and the spectral shift of the emission signal of flavins. Hence, with multispectral fluorescence lifetime imaging, we get five independent parameters, of which three are related to flavins.</p><p><strong>Conclusions: </strong>The approach to probe the metabolic state of cells in culture and spheroids using excitation at a single wavelength of 750 nm and a fluorescence time-resolved spectral detection with the consequent global analysis of the data not only simplifies image acquisition protocol but also allows to disentangle the impacts of free and bound NAD(P)H, and flavin components evaluate changes in their fluorescence parameters (emission spectra and fluorescence lifetime) upon treating cells with metabolic inhibitors and sense metabolic heterogeneity within 3D tumor spheroids.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 10","pages":"106501"},"PeriodicalIF":3.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11440180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual optical elastography detects TGF - β -induced alterations in the biomechanical properties of skin scaffolds.","authors":"Taye T Mekonnen,Yogeshwari S Ambekar,Christian Zevallos-Delgado,Achuth Nair,Fernando Zvietcovich,Hoda Zarkoob,Manmohan Singh,Yi Wei Lim,Marc Ferrer,Salavat R Aglyamov,Giuliano Scarcelli,Min Jae Song,Kirill V Larin","doi":"10.1117/1.jbo.29.9.095002","DOIUrl":"https://doi.org/10.1117/1.jbo.29.9.095002","url":null,"abstract":"SignificanceThe skin's mechanical properties are tightly regulated. Various pathologies can affect skin stiffness, and understanding these changes is a focus in tissue engineering. Ex vivo skin scaffolds are a robust platform for evaluating the effects of various genetic and molecular interactions on the skin. Transforming growth factor-beta ( TGF - β ) is a critical signaling molecule in the skin that can regulate the amount of collagen and elastin in the skin and, consequently, its mechanical properties.AimThis study investigates the biomechanical properties of bio-engineered skin scaffolds, focusing on the influence of TGF - β , a signaling molecule with diverse cellular functions.ApproachThe TGF - β receptor I inhibitor, galunisertib, was employed to assess the mechanical changes resulting from dysregulation of TGF - β . Skin scaffold samples, grouped into three categories (control, TGF - β -treated, and TGF - β + galunisertib-treated), were prepared in two distinct culture media-one with aprotinin (AP) and another without. Two optical elastography techniques, namely wave-based optical coherence elastography (OCE) and Brillouin microscopy, were utilized to quantify the biomechanical properties of the tissues.ResultsResults showed significantly higher wave speed (with AP, p < 0.001 ; without AP, p < 0.001 ) and Brillouin frequency shift (with AP, p < 0.001 ; without AP, p = 0.01 ) in TGF - β -treated group compared with the control group. The difference in wave speed between the control and TGF - β + galunisertib with ( p = 0.10 ) and without AP ( p = 0.36 ) was not significant. Moreover, the TGF - β + galunisertib-treated group exhibited lower wave speed without and with AP and reduced Brillouin frequency shift than the TGF - β -treated group without AP, further strengthening the potential role of TGF - β in regulating the mechanical properties of the samples.ConclusionsThese findings offer valuable insights into TGF - β -induced biomechanical alterations in bio-engineered skin scaffolds, highlighting the potential of OCE and Brillouin microscopy in the development of targeted therapies in conditions involving abnormal tissue remodeling and fibrosis.","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"9 1","pages":"095002"},"PeriodicalIF":3.5,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PoLambRimetry: a multispectral polarimetric atlas of lamb brain.","authors":"Verónica Mieites,Giulio Anichini,Ji Qi,Kevin O'Neill,Olga M Conde,Daniel S Elson","doi":"10.1117/1.jbo.29.9.096002","DOIUrl":"https://doi.org/10.1117/1.jbo.29.9.096002","url":null,"abstract":"SignificanceMueller matrix imaging (MMI) is a comprehensive form of polarization imaging useful for assessing structural changes. However, there is limited literature on the polarimetric properties of brain specimens, especially with multispectral analysis.AimWe aim to employ multispectral MMI for an exhaustive polarimetric analysis of brain structures, providing a reference dataset for future studies and enhancing the understanding of brain anatomy for clinicians and researchers.ApproachA multispectral wide-field MMI system was used to measure six fresh lamb brain specimens. Multiple decomposition methods (forward polar, symmetric, and differential) and polarization invariants (indices of polarimetric purity and anisotropy coefficients) have been calculated to obtain a complete polarimetric description of the samples. A total of 16 labels based on major brain structures, including grey matter (GM) and white matter (WM), were identified. K -nearest neighbors classification was used to distinguish between GM and WM and validate the feasibility of MMI for WM identification.ResultsAs the wavelength increases, both depolarization and retardance increase, suggesting enhanced tissue penetration into deeper layers. Moreover, utilizing multiple wavelengths allowed us to track dynamic shifts in the optical axis of retardance within the brain tissue, providing insights into morphological changes in WM beneath the cortical surface. The use of multispectral data for classification outperformed all results obtained with single-wavelength data and provided over 95% accuracy for the test dataset.ConclusionsThe consistency of these observations highlights the potential of multispectral wide-field MMI as a non-invasive and effective technique for investigating the brain's architecture.","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"18 1","pages":"096002"},"PeriodicalIF":3.5,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142269341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monte Carlo simulation of spatial frequency domain imaging for breast tumors during compression.","authors":"Constance M Robbins,Kuanren Qian,Yongjie Jessica Zhang,Jana M Kainerstorfer","doi":"10.1117/1.jbo.29.9.096001","DOIUrl":"https://doi.org/10.1117/1.jbo.29.9.096001","url":null,"abstract":"SignificanceNear-infrared optical imaging methods have shown promise for monitoring response to neoadjuvant chemotherapy (NAC) for breast cancer, with endogenous contrast coming from oxy- and deoxyhemoglobin. Spatial frequency domain imaging (SFDI) could be used to detect this contrast in a low-cost and portable format, but it has limited imaging depth. It is possible that local tissue compression could be used to reduce the effective tumor depth.AimTo evaluate the potential of SFDI for therapy response prediction, we aim to predict how changes to tumor size, stiffness, and hemoglobin concentration would be reflected in contrast measured by SFDI under tissue compression.ApproachFinite element analysis of compression on an inclusion-containing soft material is combined with Monte Carlo simulation to predict the measured optical contrast.ResultsWhen the effect of compression on blood volume is not considered, contrast gain from compression increases with the size and stiffness of the inclusion and decreases with the inclusion depth. With a model of reduction of blood volume from compression, compression reduces imaging contrast, an effect that is greater for larger inclusions and stiffer inclusions at shallower depths.ConclusionsThis computational modeling study represents a first step toward tracking tumor changes induced by NAC using SFDI and local compression.","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"7 1","pages":"096001"},"PeriodicalIF":3.5,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transportable hyperspectral imaging setup based on fast, high-density spectral scanning for <i>in situ</i> quantitative biochemical mapping of fresh tissue biopsies.","authors":"Luca Giannoni, Marta Marradi, Kevin Scibilia, Ivan Ezhov, Camilla Bonaudo, Angelos Artemiou, Anam Toaha, Frédéric Lange, Charly Caredda, Bruno Montcel, Alessandro Della Puppa, Ilias Tachtsidis, Daniel Rückert, Francesco Saverio Pavone","doi":"10.1117/1.JBO.29.9.093508","DOIUrl":"10.1117/1.JBO.29.9.093508","url":null,"abstract":"<p><strong>Significance: </strong>Histopathological examination of surgical biopsies, such as in glioma and glioblastoma resection, is hindered in current clinical practice by the long time required for the laboratory analysis and pathological screening, typically taking several days or even weeks to be completed.</p><p><strong>Aim: </strong>We propose here a transportable, high-density, spectral scanning-based hyperspectral imaging (HSI) setup, named HyperProbe1, that can provide <i>in situ</i>, fast biochemical analysis, and mapping of fresh surgical tissue samples, right after excision, and without the need for fixing, staining nor compromising the integrity of the tissue properties.</p><p><strong>Approach: </strong>HyperProbe1 is based on spectral scanning via supercontinuum laser illumination filtered with acousto-optic tunable filters. Such methodology allows the user to select any number and type of wavelength bands in the visible and near-infrared range between 510 and 900 nm (up to a maximum of 79) and to reconstruct 3D hypercubes composed of high-resolution (4 to <math><mrow><mn>5</mn> <mtext>  </mtext> <mi>μ</mi> <mi>m</mi></mrow> </math> ), widefield images ( <math><mrow><mn>0.9</mn> <mo>×</mo> <mn>0.9</mn> <mtext>  </mtext> <msup><mrow><mi>mm</mi></mrow> <mrow><mn>2</mn></mrow> </msup> </mrow> </math> ) of the surgical samples, where each pixel is associated with a complete spectrum.</p><p><strong>Results: </strong>The HyperProbe1 setup is here presented and characterized. The system is applied to 11 fresh surgical biopsies of glioma from routine patients, including different grades of tumor classification. Quantitative analysis of the composition of the tissue is performed via fast spectral unmixing to reconstruct the mapping of major biomarkers, such as oxy-( <math> <mrow> <msub><mrow><mi>HbO</mi></mrow> <mrow><mn>2</mn></mrow> </msub> </mrow> </math> ) and deoxyhemoglobin (HHb), as well as cytochrome-c-oxidase (CCO). We also provided a preliminary attempt to infer tumor classification based on differences in composition in the samples, suggesting the possibility of using lipid content and differential CCO concentrations to distinguish between lower and higher-grade gliomas.</p><p><strong>Conclusions: </strong>A proof of concept of the performances of HyperProbe1 for quantitative, biochemical mapping of surgical biopsies is demonstrated, paving the way for improving current post-surgical, histopathological practice via non-destructive, <i>in situ</i> streamlined screening of fresh tissue samples in a matter of minutes after excision.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 9","pages":"093508"},"PeriodicalIF":3.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}