Journal of Cell Biology最新文献_第5页

Activity-dependent astrocytic cholesterol release fine-tunes modes of synaptic exo- and endocytosis. 活动依赖性星形细胞胆固醇释放微调突触外吞和内吞模式。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-08-04 DOI: 10.1083/jcb.202512026

Jongyun Myeong, Vitaly A Klyachko

{"title":"Activity-dependent astrocytic cholesterol release fine-tunes modes of synaptic exo- and endocytosis.","authors":"Jongyun Myeong, Vitaly A Klyachko","doi":"10.1083/jcb.202512026","DOIUrl":"10.1083/jcb.202512026","url":null,"abstract":"Cholesterol is essential for the organization of neurotransmitter release machinery, yet how it regulates the balance among different forms of synaptic exo- and endocytosis remains poorly understood. Moreover, which pre-synaptic processes rely on neuronal vs. astrocyte-derived cholesterol is unknown. Using nanoscale-precision imaging of single-vesicle release in hippocampal synapses, we demonstrate that astrocytic cholesterol is a critical determinant of both temporal and spatial aspects of presynaptic dynamics by differentially modulating the two main forms of synchronous release, univesicular (UVR) and multivesicular (MVR), effectively fine-tuning their balance. Moreover, astrocytic cholesterol determines the spatial distribution of vesicle release across the active zone and the balance of the two main forms of single-vesicle endocytosis, fast and ultrafast. Imaging of a cholesterol biosensor revealed that astrocyte-neuron cholesterol signaling is activity dependent. These findings suggest that astrocytic cholesterol release modulates synaptic strength in an activity-dependent manner to fine-tune the balance of different forms of synaptic vesicle exo- and endocytosis.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148669547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Terno, a cyclin-interacting protein regulating meiotic exit and sperm differentiation in Drosophila. Terno，一种调节果蝇减数分裂退出和精子分化的周期蛋白相互作用蛋白。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-08-13 DOI: 10.1083/jcb.202511191

Elisabetta Bucciarelli, Lucia Graziadio, Claudia Pellacani, Maria Patrizia Somma, Caterina Mencarelli, Silvia Bonaccorsi, Maurizio Gatti

引用次数: 0

Lipid-mediated activation of BLT2 promotes membrane repair to prevent cell death. 脂质介导的BLT2激活促进膜修复，防止细胞死亡。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-09-03 DOI: 10.1083/jcb.202510004

Yuan Chi, Kazuko Saeki, Ken Yasukawa, Soichiro Kakuta, Tomoaki Koga, Takehiko Yokomizo

{"title":"Lipid-mediated activation of BLT2 promotes membrane repair to prevent cell death.","authors":"Yuan Chi, Kazuko Saeki, Ken Yasukawa, Soichiro Kakuta, Tomoaki Koga, Takehiko Yokomizo","doi":"10.1083/jcb.202510004","DOIUrl":"https://doi.org/10.1083/jcb.202510004","url":null,"abstract":"Various pathogenic microorganisms produce toxins that create pores in cell membranes, causing cell damage and disrupting the host epithelial barrier. Recently, we reported that mice lacking the G protein-coupled receptor leukotriene B4 receptor 2 (BLT2), which is expressed in vascular endothelial and alveolar epithelial cells, are highly susceptible to pneumolysin (PLY), a pneumococci-generated toxin. Although we clarified the protective roles of BLT2 in vascular endothelial cells, those in alveolar epithelial cells have not been elucidated. Here, we report that lipid mediator 12-hydroxyheptadecatrienoic acid (12-HHT), which is produced by membrane-damaged epithelial cells, prevents cell death by promoting membrane repair through BLT2. BLT2 promoted the release of PLY-bound plasma membranes as extracellular vesicles in a sphingomyelinase-dependent manner. Additionally, BLT2 activated Rac1 and subsequent actin polymerization, leading to resistance to cell death. Furthermore, inhibition of 12-HHT production by aspirin and treatment with a BLT2 antagonist abolished the protective effect of BLT2. These findings provide a new therapeutic strategy for bacterial infection.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatially ordered zygotic genome activation fulfills embryo quality control. 空间有序的合子基因组激活实现了胚胎质量控制。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-09-08 DOI: 10.1083/jcb.202412222

Wenchao Qian, Hui Chen, Hongju Lee, Matthew C Good

{"title":"Spatially ordered zygotic genome activation fulfills embryo quality control.","authors":"Wenchao Qian, Hui Chen, Hongju Lee, Matthew C Good","doi":"10.1083/jcb.202412222","DOIUrl":"10.1083/jcb.202412222","url":null,"abstract":"Early embryo development features autonomous, maternally driven cell divisions that self-organize the multicellular blastula or blastocyst tissue. Maternal control cedes to the zygote starting with the onset of widespread zygotic genome activation (ZGA), which is essential for subsequent cell fate determination and morphogenesis. Intriguingly, although ZGA onset is highly regulated at the level of the whole embryo, it can be non-homogenous and precisely patterned at the single-cell level. We previously demonstrated a stereotyped spatial and temporal ordering of ZGA in a model vertebrate embryo. Unknown, however, was whether this precise ZGA patterning was required for development. To address this fundamental question, we devised a strategy to spatially control cell divisions that perturb blastula embryo organization. We demonstrate the feasibility of spatially inverting the cell size pattern of embryos and find that these inverted embryos exhibit a flipped pattern of ZGA. Mispatterned ZGA along the animal-vegetal axis triggers embryo apoptosis, revealing that gastrula embryos have a built-in quality control system to sense inappropriate ZGA patterning, including regionalized defects in transcriptional onset. The quality control response is nonautonomous, dependent on an anti-apoptotic signal that suppresses cell death outside the animal hemisphere. These results reveal the requirement of properly patterned ZGA for normal development and the existence of a surveillance system of embryo quality control exquisitely tuned to the spatial and temporal ordering of genome activation and zygotic gene expression.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Loss of ErbB3 redirects Integrin β1 from early endosomal recycling to secretion in extracellular vesicles. 更正：ErbB3缺失将整合素β1从早期内体循环转向细胞外囊泡分泌。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-09-18 DOI: 10.1083/jcb.20250125508252026c

Dorival Mendes Rodrigues-Junior, Ana Rosa Sáez-Ibáñez, Julia M Scheffler, Takeshi Terabayashi, Nina Daubel, Taija Mäkinen, Olof Idevall-Hagren, Aristidis Moustakas, Ingvar Ferby

引用次数: 0

Volume EM reveals desmosome three-dimensional architecture in epithelial cells and tissue models. 体积EM显示上皮细胞和组织模型中的桥粒三维结构。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-08-05 DOI: 10.1083/jcb.202602140

Navaneetha Krishnan Bharathan, William Giang, Eunice C Chen, Stephanie E Zimmer, Sonam Lhamo, Danielle M Jorgens, Jamie L Inman, Vito Mennella, Manfred Auer, Andrew P Kowalczyk

{"title":"Volume EM reveals desmosome three-dimensional architecture in epithelial cells and tissue models.","authors":"Navaneetha Krishnan Bharathan, William Giang, Eunice C Chen, Stephanie E Zimmer, Sonam Lhamo, Danielle M Jorgens, Jamie L Inman, Vito Mennella, Manfred Auer, Andrew P Kowalczyk","doi":"10.1083/jcb.202602140","DOIUrl":"10.1083/jcb.202602140","url":null,"abstract":"Desmosomes are cell-cell adhesive junctions present in cardiac tissues and epithelial tissues such as the epidermis. These junctions anchor to the intermediate filament cytoskeleton, providing mechanical integrity. Our understanding of desmosome architecture has largely been influenced by observations of two-dimensional images obtained through conventional electron microscopy. Here, using focused ion beam scanning electron microscopy, we report the three-dimensional ultrastructure of desmosomes in A431 epidermoid carcinoma and S1 human mammary epithelial cells. We also reveal differences in desmosome ultrastructure at homotypic and heterotypic junctions of human airway epithelial cells. Quantitative analyses of these datasets reveal variations in desmosome size, shape, and organization. Importantly, we report the presence of discontinuities or \"gaps\" within the outer dense plaque, a novel feature observed in either one or both desmosome halves. This study provides the first comprehensive description of the epithelial desmosome as a three-dimensional structure and emphasizes the need to investigate the effects of dynamic morphogenetic processes and disease states on desmosome ultrastructure.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The UPR drives gametogenesis via an unexpected response, coordinating translation and ER structure. UPR通过意想不到的反应、协调翻译和ER结构驱动配子发生。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-08-20 DOI: 10.1083/jcb.202509100

Constantine Bartolutti, Alena Lauren Bishop, Yanzhe Ma, Kelsey Van Dalfsen, Elçin Ünal, Marko Jovanovic, Gloria Ann Brar

{"title":"The UPR drives gametogenesis via an unexpected response, coordinating translation and ER structure.","authors":"Constantine Bartolutti, Alena Lauren Bishop, Yanzhe Ma, Kelsey Van Dalfsen, Elçin Ünal, Marko Jovanovic, Gloria Ann Brar","doi":"10.1083/jcb.202509100","DOIUrl":"10.1083/jcb.202509100","url":null,"abstract":"Stress responses, including the unfolded protein response (UPR), are commonly studied via induction with harsh exogenous stressors, leaving endogenous functions of these pathways less well understood. We found that the endogenous UPR that precedes meiosis in budding yeast is required for gamete production but diverges dramatically from previously defined UPR outputs, with only a few characterized UPR targets induced, and mildly. The role of this UPR can be replaced by increasing ER chaperones, reducing bulk translation, or impairing the machinery for protein translocation into the ER. ER integrity appears compromised in premeiotic cells lacking the UPR, as foci of reticulon proteins are seen and correlate strongly with an inability of cells to enter meiosis. These findings indicate that physiological UPR activation supports proteostasis and normal ER structure, preparing cells for meiotic entry by reducing the load of proteins that enter the ER. Overall, our study reveals surprising features of a physiological UPR induction that enables a cell-fate decision.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A structural model for the autoinhibition of the γ-TuRC–activating CM1 domain γ- turc活化CM1结构域自抑制的结构模型

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-09-30 DOI: 10.1083/jcb.202605160

Abir Elfarkouchi,Arthur Vitard,Léa Mammri,Sébastien Brier,Lola Tanneur,Cécile Hu,Aurélien Thureau,Nicolas Joly,Francoise Ochsenbein,Paul T. Conduit

{"title":"A structural model for the autoinhibition of the γ-TuRC–activating CM1 domain","authors":"Abir Elfarkouchi,Arthur Vitard,Léa Mammri,Sébastien Brier,Lola Tanneur,Cécile Hu,Aurélien Thureau,Nicolas Joly,Francoise Ochsenbein,Paul T. Conduit","doi":"10.1083/jcb.202605160","DOIUrl":"https://doi.org/10.1083/jcb.202605160","url":null,"abstract":"Microtubule nucleation by γ-tubulin ring complexes (γ-TuRCs) must be spatiotemporally controlled to avoid major cell division errors. γ-TuRCs are activated by the centrosome-specific binding of a highly conserved centrosomin motif 1 (CM1) domain, but how binding is prevented in the cytosol remains unclear. We provide the first structural explanation for CM1 autoinhibition, revealing that stable intramolecular interactions mediate cooperative folding to block γ-TuRC–binding interfaces. Using various structural approaches, we show that CM1 forms a conserved dimeric coiled-coil hairpin that sequesters a key phenylalanine residue essential for γ-TuRC binding. This appears sufficient for autoinhibition in humans, but in flies autoinhibition requires the association of an upstream CM1 autoinhibition (CAI) domain, which forms a 4-helix dimer that covers the opposite phenylalanine residue of the hairpin dimer. Collectively, the data provide the first structural model for CM1 autoinhibition and reveal evolutionary differences across species.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"21 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subtlety is underrated: Physiological UPR activation in meiosis 细微之处被低估了：减数分裂中的生理UPR激活

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-09-23 DOI: 10.1083/jcb.202608015

David Pincus,Vladimir Denic

引用次数: 0

Rhomboid protease Rhbdl2 influences macrophage recruitment and wound repair in zebrafish 菱形蛋白酶Rhbdl2影响斑马鱼巨噬细胞募集和伤口修复

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-09-11 DOI: 10.1083/jcb.202602106

Saroj Gourkanti,Gayathri Ramakrishnan,Jacqueline Cheung,Taylor J. Schoen,Yazmin Munoz,Rosa M. Chavez,Jan Dohnálek,Katie Martin,Matthew Lovett-Barron,Thomas Whisenant,Kvido Strisovsky,Sonya E. Neal

{"title":"Rhomboid protease Rhbdl2 influences macrophage recruitment and wound repair in zebrafish","authors":"Saroj Gourkanti,Gayathri Ramakrishnan,Jacqueline Cheung,Taylor J. Schoen,Yazmin Munoz,Rosa M. Chavez,Jan Dohnálek,Katie Martin,Matthew Lovett-Barron,Thomas Whisenant,Kvido Strisovsky,Sonya E. Neal","doi":"10.1083/jcb.202602106","DOIUrl":"https://doi.org/10.1083/jcb.202602106","url":null,"abstract":"Wound repair requires tight control of immune cell behavior, yet the mechanisms that restrain immune-driven wound repair responses remain poorly defined. Here, we demonstrate that rhomboid intramembrane serine protease Rhbdl2 influences wound repair in zebrafish. We generated rhbdl2 mutants using CRISPR-Cas9 and found that, although Rhbdl2 is dispensable for normal development, its loss triggers enhanced wound repair following injury. This regenerative phenotype is accompanied by increased macrophage migration speed and accumulation at the wound site, as well as elevated early apoptosis and cell proliferation. Proteomic analyses reveal increased Rac2 protein levels in rhbdl2 mutants, which was previously identified as a regulator of leukocyte motility. Functionally, Rac2 morpholino–mediated knockdown in rhbdl2 mutant larvae suppresses the elevated macrophage recruitment and enhanced tissue repair phenotype. Together, these findings identify Rhbdl2 as a modulator of macrophage recruitment to the wound site during tissue repair, with implications for inflammatory disease, fibrosis, and tumor–immune interactions.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"7 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148932596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0