Journal of Cell Biology最新文献_第4页

SEPTIN9 locally activates the RhoGEF ARHGEF18 to promote early stages of mitochondrial fission. SEPTIN9局部激活RhoGEF ARHGEF18，促进线粒体裂变的早期阶段。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2025-09-08 DOI: 10.1083/jcb.202406017

Rachel Shannon,Yadu Balachandran,Xindi Wang,Maxime Boutry,Hong Xie,Peter K Kim,William S Trimble

引用次数: 0

Multiple interactions recruit BLTP2 to ER-PM contacts to control plasma membrane dynamics. 多重相互作用将BLTP2招募到ER-PM接触中以控制质膜动力学。

IF 6.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-03 DOI: 10.1083/jcb.202504027

Anbang Dai, Peng Xu, Chase Amos, Kenshiro Fujise, Yumei Wu, Han Yang, Julia N Eisen, Andrés Guillén-Samander, Pietro De Camilli

引用次数: 0

Nonmuscle myosin II regulates presynaptic actin and neuronal mechanobiology in Drosophila. 非肌球蛋白II调节果蝇突触前肌动蛋白和神经元力学生物学。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-07-11 DOI: 10.1083/jcb.202501211

Biljana Ermanoska, Jonathan Baets, Avital A Rodal

{"title":"Nonmuscle myosin II regulates presynaptic actin and neuronal mechanobiology in Drosophila.","authors":"Biljana Ermanoska, Jonathan Baets, Avital A Rodal","doi":"10.1083/jcb.202501211","DOIUrl":"10.1083/jcb.202501211","url":null,"abstract":"Neuromuscular junctions (NMJs) are evolutionarily ancient, specialized contacts between neurons and muscles. They experience lifelong strain, yet the mechanism preserving their integrity under mechanical load remains unclear. Here, we identify a novel actomyosin structure at Drosophila larval NMJs, consisting of a long-lived, low-turnover presynaptic actin core that colocalizes with nonmuscle myosin II (NMII) and becomes disorganized upon manipulating neuronal NMII levels or activity. Intriguingly, neuronal NMII depletion altered postsynaptic NMII levels and organization near synapses, suggesting transsynaptic propagation of actomyosin rearrangements. Under these conditions, integrin adhesion receptors were reduced on both sides of the synapse, indicating disrupted neuron-muscle connections. Notably, axon severing mimics these effects, while axonal stretching reorganizes integrins without disrupting the actin core, suggesting that presynaptic actomyosin and integrin organization are highly sensitive to mechanical cues and dynamically adjust to both loss and gain of tension. Our study reveals a presynaptic actomyosin assembly that maintains mechanical continuity between neurons and muscle, potentially enabling mechanotransduction at the NMJ through integrin-mediated adhesion.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"224 9","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144612166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of genome instability from cellular folate stress. 细胞叶酸胁迫下基因组不稳定的机制。

IF 6.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-08-18 DOI: 10.1083/jcb.202502205

Christopher Mellor, Elisabeth A Larson, Meng Wang

引用次数: 0

When huffing and puffing Ca2+ goes global, breast cancer cells are unmoved. 当Ca2+扩散到全球时，乳腺癌细胞却不为所动。

IF 6.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-08-19 DOI: 10.1083/jcb.202507062

Woo Young Chung, Shmuel Muallem

引用次数: 0

CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization. CDR2是由运动蛋白募集的动力蛋白适配体，调节内质网片的组织。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-07-10 DOI: 10.1083/jcb.202411034

Vanessa Teixeira, Kashish Singh, José B Gama, Matilde Moreira, Ricardo Celestino, Ana Xavier Carvalho, Paulo S Pereira, Carla M C Abreu, Tiago J Dantas, Andrew P Carter, Reto Gassmann

{"title":"CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization.","authors":"Vanessa Teixeira, Kashish Singh, José B Gama, Matilde Moreira, Ricardo Celestino, Ana Xavier Carvalho, Paulo S Pereira, Carla M C Abreu, Tiago J Dantas, Andrew P Carter, Reto Gassmann","doi":"10.1083/jcb.202411034","DOIUrl":"10.1083/jcb.202411034","url":null,"abstract":"The ER relies on the microtubule cytoskeleton for the organization of its extended membrane network, but how microtubule-based motors contribute remains unclear. Using biochemical and cell-based assays, we identify cerebellar degeneration-related protein 2 (CDR2) and its paralog CDR2-like (CDR2L), onconeural antigens with poorly understood functions, as ER adaptors for cytoplasmic dynein-1 (dynein). We demonstrate in human cancer cells that CDR2 is recruited by the integral ER membrane protein kinectin (KTN1) and that double knockout of CDR2 and CDR2L enhances KTN1-dependent ER sheet stacking, reversal of which by exogenous CDR2 requires its dynein-binding CC1 box motif. Exogenous CDR2 expression additionally promotes CC1 box-dependent clustering of ER sheets near centrosomes. CDR2 competes with the eEF1Bβ subunit of translation elongation factor 1 for binding to KTN1, and eEF1Bβ knockdown increases endogenous CDR2 levels on ER sheets, inducing their centrosome-proximal clustering. Our study describes a novel molecular pathway that implicates dynein in ER sheet organization and may be involved in the pathogenesis of paraneoplastic cerebellar degeneration.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"224 9","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionarily diverse caveolins share a common structural framework built around amphipathic disks. 进化上不同的洞穴蛋白有一个共同的结构框架，围绕着两性圆盘。

IF 6.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-08-07 DOI: 10.1083/jcb.202411175

Bing Han, Sarah M Connolly, Darrin T Schultz, Louis F L Wilson, Alican Gulsevin, Jens Meiler, Erkan Karakas, Melanie D Ohi, Anne K Kenworthy

{"title":"Evolutionarily diverse caveolins share a common structural framework built around amphipathic disks.","authors":"Bing Han, Sarah M Connolly, Darrin T Schultz, Louis F L Wilson, Alican Gulsevin, Jens Meiler, Erkan Karakas, Melanie D Ohi, Anne K Kenworthy","doi":"10.1083/jcb.202411175","DOIUrl":"10.1083/jcb.202411175","url":null,"abstract":"Caveolins are a unique family of membrane remodeling proteins present broadly across animals (Metazoa), and in vertebrates form flask-shaped invaginations known as caveolae. While human caveolin-1 assembles into an amphipathic disk composed of 11 spirally packed protomers, the structural basis underlying caveolin function across animals remains elusive. Here, we predicted structures for 73 caveolins spanning animal diversity, as well as a newly identified choanoflagellate caveolin from Salpingoeca rosetta. This analysis revealed seven conserved structural elements and a propensity to assemble into amphipathic disks. Cryo-EM structures of caveolins from S. rosetta choanoflagellate and the purple sea urchin Strongylocentrotus purpuratus exhibit striking structural similarities to human caveolin-1, validating the structural predictions. Lastly, tracing the chromosomal evolutionary history of caveolins revealed its parahoxozoan ancestral chromosome and evolutionary branches on which caveolins translocated and expanded. These results show that caveolins possess an ancient structural framework predating Metazoa and provide a new structural paradigm to explore the molecular basis of caveolin function across diverse evolutionary lineages.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"224 9","pages":""},"PeriodicalIF":6.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12330381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOMO no more: NOMO and calmin extend mechanobiology to the ER. 不再有FOMO: NOMO和calm将机械生物学扩展到急诊室。

IF 6.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-08-18 DOI: 10.1083/jcb.202508037

Julie Heffler, Jan Lammerding

引用次数: 0

Nodal modulator (NOMO) is a force-bearing transmembrane protein required for muscle differentiation. Nodal modulator （NOMO）是肌肉分化所需的一种力承受力跨膜蛋白。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-07-15 DOI: 10.1083/jcb.202505010

Brigitte S Naughton, Swapnil C Devarkar, Vanessa Todorow, Sunanda Mallik, Stacey Oxendine, Sanjana Junnarkar, Yuan Ren, Julien Berro, Janine Kirstein, Yong Xiong, Christian Schlieker

{"title":"Nodal modulator (NOMO) is a force-bearing transmembrane protein required for muscle differentiation.","authors":"Brigitte S Naughton, Swapnil C Devarkar, Vanessa Todorow, Sunanda Mallik, Stacey Oxendine, Sanjana Junnarkar, Yuan Ren, Julien Berro, Janine Kirstein, Yong Xiong, Christian Schlieker","doi":"10.1083/jcb.202505010","DOIUrl":"10.1083/jcb.202505010","url":null,"abstract":"The ER relies on membrane-shaping proteins to maintain a continuous network of sheets and tubules that host distinct biological processes. How this intricate structure of the ER membrane system is maintained under conditions of mechanical strain is incompletely understood. NOMO is an ER-resident transmembrane protein that contributes to ER morphology and is highly expressed in striated muscle. In this study, we identify a critical interface between distal Ig domains that enables NOMO to maintain ER morphology and bear mechanical forces. By incorporating two independent tension sensors in the luminal domain of NOMO, we demonstrate that NOMO assemblies experience forces in the single piconewton range, with a significant contribution from the identified interface. These newly defined features are important-if not indispensable-for myogenesis, as interface mutations affecting mechanosensitivity fail to restore the essential role of NOMO during myogenesis in a C2C12 differentiation model. Moreover, NOMO depletion impairs nematode motility, underscoring a broader functional importance in muscle physiology.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"224 9","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12262048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arc controls organ architecture through modulation of Crb and MyoII. Arc通过调节Crb和MyoII来控制器官结构。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-06-12 DOI: 10.1083/jcb.202409078

Ji Hoon Kim, Rika Maruyama, Kwon Kim, Devin A Vertrees, Parama Paul, Kyla Britson, Nathaniel R Laughner, Deborah J Andrew

{"title":"Arc controls organ architecture through modulation of Crb and MyoII.","authors":"Ji Hoon Kim, Rika Maruyama, Kwon Kim, Devin A Vertrees, Parama Paul, Kyla Britson, Nathaniel R Laughner, Deborah J Andrew","doi":"10.1083/jcb.202409078","DOIUrl":"10.1083/jcb.202409078","url":null,"abstract":"Precise orchestration of morphogenetic processes generates organs that are optimally positioned and the right size and shape to fit and maximize functionality. Here, we show that Arc, a large apical membrane-associated PDZ domain-containing protein, works through the apical determinant Crumbs to limit non-muscle myosin II (MyoII) activity during tissue invagination in the Drosophila salivary gland (SG). We show that loss of Arc, attenuation of Crumbs, and increased activation of MyoII leads to the simultaneous internalization of more precursor cells than normal. Consequently, mature SGs are shorter with more cells surrounding the lumen all along the tube. Correspondingly, overexpression of Arc or SG-specific knockdown of MyoII leads to longer SGs with fewer cells surrounding the lumen. Our findings support a model wherein plasma membrane (PM)-associated Crumbs stabilizes cellular junctions by limiting apical pools of activated MyoII and countering the destabilizing effects of MyoII at the PM, limiting how many cells internalize at any given time, shaping final tube geometry.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"224 9","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12160935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0