Journal of Cell Biology最新文献_第10页

Procollagen 1 assembles into phase-separated condensates in the endoplasmic reticulum. 前胶原1在内质网中组装成相分离的凝聚物。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-08-03 Epub Date: 2026-06-11 DOI: 10.1083/jcb.202603129

Soumya Bhattacharyya, Jose Wojnacki, Nathalie Brouwers, Vivek Malhotra

引用次数: 0

Fibroblast depletion reveals mammalian epithelial resilience across neonatal and adult stages. 成纤维细胞耗竭揭示哺乳动物上皮在新生儿和成年阶段的恢复能力。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-08-03 Epub Date: 2026-06-12 DOI: 10.1083/jcb.202507165

Isabella M Gaeta, Shuangshuang Du, Clémentine Villeneuve, David G Gonzalez, Catherine Matte-Martone, Smirthy Ganesan, Deandra Simpson, Hilldana Tibebu, Jessica L Moore, Chen Yuan Kam, Sara Gallini, Haoyang Wei, Fabien Bertillot, Dagmar Zeuschner, Lauren E Gonzalez, Ushnish Rana, Kaelyn D Sumigray, Sara A Wickström, Valentina Greco

{"title":"Fibroblast depletion reveals mammalian epithelial resilience across neonatal and adult stages.","authors":"Isabella M Gaeta, Shuangshuang Du, Clémentine Villeneuve, David G Gonzalez, Catherine Matte-Martone, Smirthy Ganesan, Deandra Simpson, Hilldana Tibebu, Jessica L Moore, Chen Yuan Kam, Sara Gallini, Haoyang Wei, Fabien Bertillot, Dagmar Zeuschner, Lauren E Gonzalez, Ushnish Rana, Kaelyn D Sumigray, Sara A Wickström, Valentina Greco","doi":"10.1083/jcb.202507165","DOIUrl":"10.1083/jcb.202507165","url":null,"abstract":"<p><p>Regenerative organs, like the skin, depend on niche-stem cell interactions that sustain cellular turnover. In culture, skin fibroblasts promote epidermal stem cell proliferation and differentiation. Yet, it remains elusive how fibroblasts regulate epidermal stem cell behaviors and differentiation in vivo in skin. Here, we asked how fibroblast depletion may impact proliferation of the epidermal stem cell compartment. Surprisingly, we find that significant depletion of fibroblast density does not affect epidermal stem cell proliferation during adult or neonatal stages in vivo. These results demonstrate that across different ages, proliferation of epidermal stem cells can persist in the face of a depleted fibroblast population. Interestingly, neonatal fibroblast depletion does not significantly reduce collagen I density but affects basement membrane mechanics and epidermal stem cell delamination. Despite these changes, the skin continues to maintain its protective barrier function. Thus, our work demonstrates the skin regenerative program employs robust compensatory mechanisms in response to fibroblast depletion to maintain functional capacity.</p>","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 8","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13267860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148225079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The missing link: Piccolino is essential for tethering synaptic vesicles to rod photoreceptor ribbons. 缺失的一环：短笛是将突触囊泡系在杆状光感受器带上所必需的。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-07-23 DOI: 10.1083/jcb.202509110

Kaspar Gierke,Michalina Gadomska,Julia Breuer,Julius N Bahr,Tanja M Müller,Hanna Ehnis,Sina Zobel,Nancy Mejia Villagran,Sonja A Kirsch,Alexandra Skrzypek,Renato Frischknecht,Anna Fejtová,Rainer A Böckmann,Carolin Wichmann,Hanna Regus-Leidig,Johann Helmut Brandstätter

{"title":"The missing link: Piccolino is essential for tethering synaptic vesicles to rod photoreceptor ribbons.","authors":"Kaspar Gierke,Michalina Gadomska,Julia Breuer,Julius N Bahr,Tanja M Müller,Hanna Ehnis,Sina Zobel,Nancy Mejia Villagran,Sonja A Kirsch,Alexandra Skrzypek,Renato Frischknecht,Anna Fejtová,Rainer A Böckmann,Carolin Wichmann,Hanna Regus-Leidig,Johann Helmut Brandstätter","doi":"10.1083/jcb.202509110","DOIUrl":"https://doi.org/10.1083/jcb.202509110","url":null,"abstract":"Retinal photoreceptors transmit light signals to their postsynaptic neurons with high precision, speed and without fatigue. This high-throughput neurotransmission relies on a sophisticated molecular machinery centered on a presynaptic organelle, the synaptic ribbon (SR). A hallmark of SRs is the recruitment of synaptic vesicles (SVs) from the cytoplasmic SV pool via \"tethering\". However, the identity of the tether and the mechanism underlying SV tethering are unknown. Here, we show that cell-specific deletion of the SR-associated protein Piccolino from rod photoreceptors disrupts SR morphology and ablates SV tethering. Nanoscale epitope mapping suggests that Piccolino acts as an SV tether by extending its N terminus away from the SR into the SV-filled terminal cytoplasm. With in silico modeling and protein lipid-binding assays, we demonstrate that an amphipathic liquid packing sensor motif (ALPS) at the N terminus of Piccolino binds SV-like liposomes, implicating this interaction as the mechanism underlying SV tethering. Together, our findings identified Piccolino as the molecular link between the SR and SVs.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"22 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148552525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Septins and cytokinesis in the polymorphic fungus Aureobasidium pullulans. 多态真菌普鲁兰小孢子菌的酶和细胞分裂。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-07-17 DOI: 10.1083/jcb.202601114

Analeigha V Colarusso,Alison C E Wirshing,Daniel J Lew

引用次数: 0

Satellite view: Dissection of PCM1 assembly processes reveals centriolar satellite structures. 卫星图：PCM1装配过程的解剖揭示了向心卫星结构。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-07-17 DOI: 10.1083/jcb.202606168

Ruth M Kearney,Anushka Sharma,Ciaran G Morrison

引用次数: 0

Controlling scissors during DNA repair of UV light-induced damage. 在紫外线诱导的DNA损伤修复过程中控制剪刀。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-07-14 DOI: 10.1083/jcb.202606004

Patricia L Opresko,Orlando D Schärer

引用次数: 0

Rag GTPases and mTORC1 regulate intestinal stem cell activity in response to nutrient availability. Rag gtpase和mTORC1调节肠道干细胞活性对营养物质的响应。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-07-13 DOI: 10.1083/jcb.202510172

Weikang Fan,Churui Ge,Chunmei Niu,Shuang Liu,Peng Wang,Heng Li,Yang Cheng,Yingying Lu,Youheng Wei

{"title":"Rag GTPases and mTORC1 regulate intestinal stem cell activity in response to nutrient availability.","authors":"Weikang Fan,Churui Ge,Chunmei Niu,Shuang Liu,Peng Wang,Heng Li,Yang Cheng,Yingying Lu,Youheng Wei","doi":"10.1083/jcb.202510172","DOIUrl":"https://doi.org/10.1083/jcb.202510172","url":null,"abstract":"The intestinal epithelium, predominantly composed of enterocytes (ECs), integrates dietary cues to regulate intestinal stem cell (ISC) activity and maintain intestinal homeostasis. However, the underlying mechanisms remain incompletely understood. Here, we demonstrate the functions of essential nutrient-sensing mediators Rag GTPases and mTORC1 on Drosophila ISC activity. Inhibition of Rag GTPases in ECs triggers Mitf activation, which subsequently induces Upd3 expression to non-cell autonomously increase the ISC proliferation and differentiation through stimulating the JAK-STAT pathway. Inactivation of mTORC1 in ECs also activates the Upd3-JAK-STAT axis and increases ISC activity, but through a Mitf-independent mechanism. Moreover, in contrast to mTORC1 inactivation blocking ISC proliferation and differentiation, depletion of Rag GTPases in ISCs shows no obvious cell autonomous effect on ISC activity. Consistent with inhibition of the nutrient-responsive pathway in ECs promoting ISC proliferation, long-term nutrient starvation enhances ISC activity and Upd3 expression. Together, our findings reveal a nutrient-sensing regulatory network that controls ISC proliferation involving the Rag GTPases-Mitf axis and mTORC1 activity.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"60 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PISD acts as a switch between lipophagy and fatty acid transfer to mitochondria. PISD在脂肪吞噬和脂肪酸向线粒体转移之间起着开关作用。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-07-13 DOI: 10.1083/jcb.202605090

Hanbing Xue,Xingwen Hu,Zhenni Yang,Yiqing Cao,Qi Zhu,Hongyu Zhao,Xiaoli Ma,Tianxin Lei,Dian Zhuang,Jing Zheng,Siqi Guo,Xiaochuan Fu,Ka Kit Chung,Pingsheng Liu,Xiaohong Zhuang,Zhengyu Liang,Sicong He,Hui Jiang,Yan G Zhao

{"title":"PISD acts as a switch between lipophagy and fatty acid transfer to mitochondria.","authors":"Hanbing Xue,Xingwen Hu,Zhenni Yang,Yiqing Cao,Qi Zhu,Hongyu Zhao,Xiaoli Ma,Tianxin Lei,Dian Zhuang,Jing Zheng,Siqi Guo,Xiaochuan Fu,Ka Kit Chung,Pingsheng Liu,Xiaohong Zhuang,Zhengyu Liang,Sicong He,Hui Jiang,Yan G Zhao","doi":"10.1083/jcb.202605090","DOIUrl":"https://doi.org/10.1083/jcb.202605090","url":null,"abstract":"Fatty acids (FAs) are transported from lipid droplets (LDs) to mitochondria for β-oxidation during cell starvation. Starvation also triggers engulfment of LDs by autophagosomes and their subsequent degradation by lysosomes (lipophagy). The mechanisms coordinating these pathways remain unclear. Here, we demonstrate that PISD-LD, an LD-localized isoform of phosphatidylserine decarboxylase, facilitates FA transfer while inhibiting lipophagy. PISD-LD mediates LD-mitochondrion (LD-mito) contacts via interaction with mitochondrial PISD. In PISD-LD KD cells, LDs are larger, and FA trafficking and mitochondrial FA β-oxidation are suppressed. The lipid transfer proteins ATG2A/B are recruited by PISDs to mediate FA transfer from LDs to mitochondria. Disruption of PISD-LD-mediated LD-mito contacts activates lipophagy, aiding LD degradation. PISD-LD binds the lipophagy receptor Spartin and inhibits lipophagy by impeding Spartin-LC3 interaction. PISD-LD also regulates LD-mito contacts and lipid metabolism in mouse liver. Thus, PISD-LD serves as a switch between LD-to-mitochondrion FA transfer and lipophagy, ensuring efficient energy production.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"447 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148428920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Receptor-mediated Golgi retention of Fam20 kinases tunes secretome phosphorylation during lactation. 受体介导的Fam20激酶高尔基体保留调节泌乳期间分泌组磷酸化。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-07-13 DOI: 10.1083/jcb.202507107

Xiaotong Yang,Jueyin He,Xiulan Chen,Xi'e Wang,Chenyun Hu,Xuqian Zhao,Xinxin Chen,Jiaojiao Yu,Ping Liu,Jifeng Wang,Xi Wang,Junjie Hu,Mei Ding,Fuquan Yang,Chih-Chen Wang,Lei Wang

{"title":"Receptor-mediated Golgi retention of Fam20 kinases tunes secretome phosphorylation during lactation.","authors":"Xiaotong Yang,Jueyin He,Xiulan Chen,Xi'e Wang,Chenyun Hu,Xuqian Zhao,Xinxin Chen,Jiaojiao Yu,Ping Liu,Jifeng Wang,Xi Wang,Junjie Hu,Mei Ding,Fuquan Yang,Chih-Chen Wang,Lei Wang","doi":"10.1083/jcb.202507107","DOIUrl":"https://doi.org/10.1083/jcb.202507107","url":null,"abstract":"Fam20C, the first protein kinase identified in the secretory pathway, governs the majority phosphorylation of the secretome. Although Fam20C has a high propensity for secretion, its kinase activity occurs intracellularly. How Fam20C clients are phosphorylated inside cells remains elusive. Here, we demonstrate that the pseudokinase Fam20A forms a heterocomplex with Fam20C on the Golgi membrane, anchoring the cleaved, mature form of Fam20C within the Golgi. Their Golgi localization is promoted by a set of cargo receptors ERGIC2 and ERGIC3, which drive the ER-to-Golgi transport of Fam20A-Fam20C. Importantly, both ERGIC2 and ERGIC3 are upregulated in the mammary gland during lactation, and Ergic2 or Ergic3 knockout mice display global changes in secretome phosphorylation, less phosphorylated β-casein in milk, and deficiency in offspring growth. Our findings uncover a previously unrecognized mechanism for the spatiotemporal regulation of Fam20 kinases, crucial for efficient phosphorylation of secretory proteins during lactation.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"39 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More gas, fewer brakes: Mitochondria withhold CoQ at the cost of ferroptosis defense. 更多的气体，更少的刹车：线粒体抑制CoQ以牺牲铁下垂防御为代价。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-07-13 DOI: 10.1083/jcb.202606160

Mami Sato,Florencio Porto Freitas,José Pedro Friedmann Angeli

引用次数: 0