Journal of Cell Biology最新文献_第10页

A genome-wide screen links peroxisome regulation with Wnt signaling through RNF146 and TNKS/2. 一项全基因组筛选通过 RNF146 和 TNKS/2 将过氧物酶体调控与 Wnt 信号传导联系起来。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-07-05 DOI: 10.1083/jcb.202312069

Jonathan T Vu, Katherine U Tavasoli, Connor J Sheedy, Soham P Chowdhury, Lori Mandjikian, Julien Bacal, Meghan A Morrissey, Chris D Richardson, Brooke M Gardner

{"title":"A genome-wide screen links peroxisome regulation with Wnt signaling through RNF146 and TNKS/2.","authors":"Jonathan T Vu, Katherine U Tavasoli, Connor J Sheedy, Soham P Chowdhury, Lori Mandjikian, Julien Bacal, Meghan A Morrissey, Chris D Richardson, Brooke M Gardner","doi":"10.1083/jcb.202312069","DOIUrl":"10.1083/jcb.202312069","url":null,"abstract":"Peroxisomes are membrane-bound organelles harboring metabolic enzymes. In humans, peroxisomes are required for normal development, yet the genes regulating peroxisome function remain unclear. We performed a genome-wide CRISPRi screen to identify novel factors involved in peroxisomal homeostasis. We found that inhibition of RNF146, an E3 ligase activated by poly(ADP-ribose), reduced the import of proteins into peroxisomes. RNF146-mediated loss of peroxisome import depended on the stabilization and activity of the poly(ADP-ribose) polymerases TNKS and TNKS2, which bind the peroxisomal membrane protein PEX14. We propose that RNF146 and TNKS/2 regulate peroxisome import efficiency by PARsylation of proteins at the peroxisome membrane. Interestingly, we found that the loss of peroxisomes increased TNKS/2 and RNF146-dependent degradation of non-peroxisomal substrates, including the β-catenin destruction complex component AXIN1, which was sufficient to alter the amplitude of β-catenin transcription. Together, these observations not only suggest previously undescribed roles for RNF146 in peroxisomal regulation but also a novel role in bridging peroxisome function with Wnt/β-catenin signaling during development.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AMPK activation induces RALDH+ tolerogenic dendritic cells by rewiring glucose and lipid metabolism. AMPK 激活可通过重构葡萄糖和脂质代谢诱导 RALDH+耐受性树突状细胞。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-08-08 DOI: 10.1083/jcb.202401024

Eline C Brombacher, Thiago A Patente, Alwin J van der Ham, Tijmen J A Moll, Frank Otto, Fenne W M Verheijen, Esther A Zaal, Arnoud H de Ru, Rayman T N Tjokrodirijo, Celia R Berkers, Peter A van Veelen, Bruno Guigas, Bart Everts

{"title":"AMPK activation induces RALDH+ tolerogenic dendritic cells by rewiring glucose and lipid metabolism.","authors":"Eline C Brombacher, Thiago A Patente, Alwin J van der Ham, Tijmen J A Moll, Frank Otto, Fenne W M Verheijen, Esther A Zaal, Arnoud H de Ru, Rayman T N Tjokrodirijo, Celia R Berkers, Peter A van Veelen, Bruno Guigas, Bart Everts","doi":"10.1083/jcb.202401024","DOIUrl":"10.1083/jcb.202401024","url":null,"abstract":"Dendritic cell (DC) activation and function are underpinned by profound changes in cellular metabolism. Several studies indicate that the ability of DCs to promote tolerance is dependent on catabolic metabolism. Yet the contribution of AMP-activated kinase (AMPK), a central energy sensor promoting catabolism, to DC tolerogenicity remains unknown. Here, we show that AMPK activation renders human monocyte-derived DCs tolerogenic as evidenced by an enhanced ability to drive differentiation of regulatory T cells, a process dependent on increased RALDH activity. This is accompanied by several metabolic changes, including increased breakdown of glycerophospholipids, enhanced mitochondrial fission-dependent fatty acid oxidation, and upregulated glucose catabolism. This metabolic rewiring is functionally important as we found interference with these metabolic processes to reduce to various degrees AMPK-induced RALDH activity as well as the tolerogenic capacity of moDCs. Altogether, our findings reveal a key role for AMPK signaling in shaping DC tolerogenicity and suggest AMPK as a target to direct DC-driven tolerogenic responses in therapeutic settings.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11310580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated stress response activator halofuginone protects mice from diabetes-like phenotypes. 综合应激反应激活剂卤夫酮可保护小鼠免受糖尿病样表型的影响。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-08-16 DOI: 10.1083/jcb.202405175

Shashank Rai, Maria Szaruga, Aleksandra P Pitera, Anne Bertolotti

引用次数: 0

An evolutionarily conserved AnkyrinG-dependent motif clusters axonal K2P K+ channels. 轴突 K2P K+ 通道的一个进化保守的 AnkyrinG 依赖性基团。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-07-30 DOI: 10.1083/jcb.202401140

Gabriel Escobedo, Yu Wu, Yuki Ogawa, Xiaoyun Ding, Matthew N Rasband

{"title":"An evolutionarily conserved AnkyrinG-dependent motif clusters axonal K2P K+ channels.","authors":"Gabriel Escobedo, Yu Wu, Yuki Ogawa, Xiaoyun Ding, Matthew N Rasband","doi":"10.1083/jcb.202401140","DOIUrl":"10.1083/jcb.202401140","url":null,"abstract":"The evolution of ion channel clustering at nodes of Ranvier enabled the development of complex vertebrate nervous systems. At mammalian nodes, the K+ leak channels TRAAK and TREK-1 underlie membrane repolarization. Despite the molecular similarities between nodes and the axon initial segment (AIS), TRAAK and TREK-1 are reportedly node-specific, suggesting a unique clustering mechanism. However, we show that TRAAK and TREK-1 are enriched at both nodes and AIS through a common mechanism. We identified a motif near the C-terminus of TRAAK that is necessary and sufficient for its clustering. The motif first evolved among cartilaginous fish. Using AnkyrinG (AnkG) conditional knockout mice, CRISPR/Cas9-mediated disruption of AnkG, co-immunoprecipitation, and surface recruitment assays, we show that TRAAK forms a complex with AnkG and that AnkG is necessary for TRAAK's AIS and nodal clustering. In contrast, TREK-1's clustering requires TRAAK. Our results expand the repertoire of AIS and nodal ion channel clustering mechanisms and emphasize AnkG's central role in assembling excitable domains.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11289519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synaptotagmin-1 undergoes phase separation to regulate its calcium-sensitive oligomerization. 突触表蛋白-1通过相分离来调节其钙敏感性寡聚。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-07-09 DOI: 10.1083/jcb.202311191

Min Zhu, Han Xu, Yulei Jin, Xiaoxu Kong, Bingkuan Xu, Yinghui Liu, Haijia Yu

{"title":"Synaptotagmin-1 undergoes phase separation to regulate its calcium-sensitive oligomerization.","authors":"Min Zhu, Han Xu, Yulei Jin, Xiaoxu Kong, Bingkuan Xu, Yinghui Liu, Haijia Yu","doi":"10.1083/jcb.202311191","DOIUrl":"10.1083/jcb.202311191","url":null,"abstract":"Synaptotagmin-1 (Syt1) is a calcium sensor that regulates synaptic vesicle fusion in synchronous neurotransmitter release. Syt1 interacts with negatively charged lipids and the SNARE complex to control the fusion event. However, it remains incompletely understood how Syt1 mediates Ca2+-trigged synaptic vesicle fusion. Here, we discovered that Syt1 undergoes liquid-liquid phase separation (LLPS) to form condensates both in vitro and in living cells. Syt1 condensates play a role in vesicle attachment to the PM and efficiently recruit SNAREs and complexin, which may facilitate the downstream synaptic vesicle fusion. We observed that Syt1 condensates undergo a liquid-to-gel-like phase transition, reflecting the formation of Syt1 oligomers. The phase transition can be blocked or reversed by Ca2+, confirming the essential role of Ca2+ in Syt1 oligomer disassembly. Finally, we showed that the Syt1 mutations causing Syt1-associated neurodevelopmental disorder impair the Ca2+-driven phase transition. These findings reveal that Syt1 undergoes LLPS and a Ca2+-sensitive phase transition, providing new insights into Syt1-mediated vesicle fusion.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LYSMD proteins promote activation of Rab32-family GTPases for lysosome-related organelle biogenesis. LYSMD蛋白可促进Rab32家族GTP酶的活化，从而促进溶酶体相关细胞器的生物生成。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-07-30 DOI: 10.1083/jcb.202402016

Jinglin Li, Qiuyuan Yin, Nan Xuan, Qiwen Gan, Chaolian Liu, Qian Zhang, Mei Yang, Chonglin Yang

{"title":"LYSMD proteins promote activation of Rab32-family GTPases for lysosome-related organelle biogenesis.","authors":"Jinglin Li, Qiuyuan Yin, Nan Xuan, Qiwen Gan, Chaolian Liu, Qian Zhang, Mei Yang, Chonglin Yang","doi":"10.1083/jcb.202402016","DOIUrl":"10.1083/jcb.202402016","url":null,"abstract":"Lysosome-related organelles (LROs) are specialized lysosomes with cell type-specific roles in organismal homeostasis. Dysregulation of LROs leads to many human disorders, but the mechanisms underlying their biogenesis are not fully understood. Here, we identify a group of LYSMD proteins as evolutionarily conserved regulators of LROs. In Caenorhabditis elegans, mutations of LMD-2, a LysM domain-containing protein, reduce the levels of the Rab32 GTPase ortholog GLO-1 on intestine-specific LROs, the gut granules, leading to their abnormal enlargement and defective biogenesis. LMD-2 interacts with GLO-3, a subunit of GLO-1 guanine nucleotide exchange factor (GEF), thereby promoting GLO-1 activation. Mammalian homologs of LMD-2, LYSMD1, and LYSMD2 can functionally replace LMD-2 in C. elegans. In mammals, LYSMD1/2 physically interact with the HPS1 subunit of BLOC-3, the GEF of Rab32/38, thus promoting Rab32 activation. Inactivation of both LYSMD1 and LYSMD2 reduces Rab32 activation, causing melanosome enlargement and decreased melanin production in mouse melanoma cells. These findings provide important mechanistic insights into LRO biogenesis and functions.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11289520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells. 小 GTPase 活性分析（SAIYAN）系统：一种检测活细胞中 GTP 酶活化的方法。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-08-05 DOI: 10.1083/jcb.202403179

Miharu Maeda, Masashi Arakawa, Yukie Komatsu, Kota Saito

{"title":"Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells.","authors":"Miharu Maeda, Masashi Arakawa, Yukie Komatsu, Kota Saito","doi":"10.1083/jcb.202403179","DOIUrl":"10.1083/jcb.202403179","url":null,"abstract":"Small GTPases are essential in various cellular signaling pathways, and detecting their activation within living cells is crucial for understanding cellular processes. The current methods for detecting GTPase activation using fluorescent proteins rely on the interaction between the GTPase and its effector. Consequently, these methods are not applicable to factors, such as Sar1, where the effector also functions as a GTPase-activating protein. Here, we present a novel method, the Small GTPase ActIvitY ANalyzing (SAIYAN) system, for detecting the activation of endogenous small GTPases via fluorescent signals utilizing a split mNeonGreen system. We demonstrated Sar1 activation at the endoplasmic reticulum (ER) exit site and successfully detected its activation state in various cellular conditions. Utilizing the SAIYAN system in collagen-secreting cells, we discovered activated Sar1 localized both at the ER exit sites and ER-Golgi intermediate compartment (ERGIC) regions. Additionally, impaired collagen secretion confined the activated Sar1 at the ER exit sites, implying the importance of Sar1 activation through the ERGIC in collagen secretion.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11303508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Permanent deconstruction of intracellular primary cilia in differentiating granule cell neurons. 分化中的颗粒细胞神经元细胞内原始纤毛的永久性解构

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-08-13 DOI: 10.1083/jcb.202404038

Carolyn M Ott, Sandii Constable, Tri M Nguyen, Kevin White, Wei-Chung Allen Lee, Jennifer Lippincott-Schwartz, Saikat Mukhopadhyay

{"title":"Permanent deconstruction of intracellular primary cilia in differentiating granule cell neurons.","authors":"Carolyn M Ott, Sandii Constable, Tri M Nguyen, Kevin White, Wei-Chung Allen Lee, Jennifer Lippincott-Schwartz, Saikat Mukhopadhyay","doi":"10.1083/jcb.202404038","DOIUrl":"10.1083/jcb.202404038","url":null,"abstract":"Primary cilia on granule cell neuron progenitors in the developing cerebellum detect sonic hedgehog to facilitate proliferation. Following differentiation, cerebellar granule cells become the most abundant neuronal cell type in the brain. While granule cell cilia are essential during early developmental stages, they become infrequent upon maturation. Here, we provide nanoscopic resolution of cilia in situ using large-scale electron microscopy volumes and immunostaining of mouse cerebella. In many granule cells, we found intracellular cilia, concealed from the external environment. Cilia were disassembled in differentiating granule cell neurons-in a process we call cilia deconstruction-distinct from premitotic cilia resorption in proliferating progenitors. In differentiating granule cells, cilia deconstruction involved unique disassembly intermediates, and, as maturation progressed, mother centriolar docking at the plasma membrane. Unlike ciliated neurons in other brain regions, our results show the deconstruction of concealed cilia in differentiating granule cells, which might prevent mitogenic hedgehog responsiveness. Ciliary deconstruction could be paradigmatic of cilia removal during differentiation in other tissues.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11320830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Murine glial protrusion transcripts predict localized Drosophila glial mRNAs involved in plasticity. 小鼠神经胶质突起转录本预测了果蝇神经胶质 mRNA 的定位，这些 mRNA 与可塑性有关。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-07-22 DOI: 10.1083/jcb.202306152

Jeffrey Y Lee, Dalia S Gala, Maria Kiourlappou, Julia Olivares-Abril, Jana Joha, Joshua S Titlow, Rita O Teodoro, Ilan Davis

{"title":"Murine glial protrusion transcripts predict localized Drosophila glial mRNAs involved in plasticity.","authors":"Jeffrey Y Lee, Dalia S Gala, Maria Kiourlappou, Julia Olivares-Abril, Jana Joha, Joshua S Titlow, Rita O Teodoro, Ilan Davis","doi":"10.1083/jcb.202306152","DOIUrl":"10.1083/jcb.202306152","url":null,"abstract":"The polarization of cells often involves the transport of specific mRNAs and their localized translation in distal projections. Neurons and glia are both known to contain long cytoplasmic processes, while localized transcripts have only been studied extensively in neurons, not glia, especially in intact nervous systems. Here, we predict 1,740 localized Drosophila glial transcripts by extrapolating from our meta-analysis of seven existing studies characterizing the localized transcriptomes and translatomes of synaptically associated mammalian glia. We demonstrate that the localization of mRNAs in mammalian glial projections strongly predicts the localization of their high-confidence Drosophila homologs in larval motor neuron-associated glial projections and are highly statistically enriched for genes associated with neurological diseases. We further show that some of these localized glial transcripts are specifically required in glia for structural plasticity at the nearby neuromuscular junction synapses. We conclude that peripheral glial mRNA localization is a common and conserved phenomenon and propose that it is likely to be functionally important in disease.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11262410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical transformation of the multibudding yeast, Aureobasidium pullulans. 多芽酵母 Aureobasidium pullulans 的化学转化。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-06-27 DOI: 10.1083/jcb.202402114

Alison C E Wirshing, Claudia A Petrucco, Daniel J Lew

{"title":"Chemical transformation of the multibudding yeast, Aureobasidium pullulans.","authors":"Alison C E Wirshing, Claudia A Petrucco, Daniel J Lew","doi":"10.1083/jcb.202402114","DOIUrl":"10.1083/jcb.202402114","url":null,"abstract":"Aureobasidium pullulans is a ubiquitous polymorphic black yeast with industrial and agricultural applications. It has recently gained attention amongst cell biologists for its unconventional mode of proliferation in which multinucleate yeast cells make multiple buds within a single cell cycle. Here, we combine a chemical transformation method with genome-targeted homologous recombination to yield ∼60 transformants/μg of DNA in just 3 days. This protocol is simple, inexpensive, and requires no specialized equipment. We also describe vectors with codon-optimized green and red fluorescent proteins for A. pullulans and use these tools to explore novel cell biology. Quantitative imaging of a strain expressing cytosolic and nuclear markers showed that although the nuclear number varies considerably among cells of similar volume, total nuclear volume scales with cell volume over an impressive 70-fold size range. The protocols and tools described here expand the toolkit for A. pullulans biologists and will help researchers address the many other puzzles posed by this polyextremotolerant and morphologically plastic organism.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"223 10","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0