Journal of Cell Biology最新文献_第3页

FAPs orchestrate ANTXR2-mediated fine-tuning of collagen VI to ensure skeletal muscle function. FAPs协调antxr2介导的胶原VI微调，以确保骨骼肌功能。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-11-02 Epub Date: 2026-10-15 DOI: 10.1083/jcb.202606056

Samuele Metti, Lucie Bracq, Matteo Signor, Nicola Facchinello, Lisa Gambarotto, Leonardo Nogara, Giulia Pigato, Laurence Abrami, Béatrice Kunz, Julien Duc, Patrizia Sabatelli, Aurora De Acutis, Gabriele Maria Fortunato, Giovanni Vozzi, Paola Braghetta, Bert Blaauw, F Gisou van der Goot, Paolo Bonaldo

{"title":"FAPs orchestrate ANTXR2-mediated fine-tuning of collagen VI to ensure skeletal muscle function.","authors":"Samuele Metti, Lucie Bracq, Matteo Signor, Nicola Facchinello, Lisa Gambarotto, Leonardo Nogara, Giulia Pigato, Laurence Abrami, Béatrice Kunz, Julien Duc, Patrizia Sabatelli, Aurora De Acutis, Gabriele Maria Fortunato, Giovanni Vozzi, Paola Braghetta, Bert Blaauw, F Gisou van der Goot, Paolo Bonaldo","doi":"10.1083/jcb.202606056","DOIUrl":"https://doi.org/10.1083/jcb.202606056","url":null,"abstract":"Tissue function relies on the ECM, which surrounds cells and provides structural and biochemical support. Maintaining ECM homeostasis requires a tightly regulated balance between deposition and degradation of its components, and disruption of this equilibrium can lead to disease. Here, we examined the role of anthrax toxin receptor 2 (ANTXR2/CMG2), an ECM turnover receptor whose loss-of-function mutations cause hyaline fibromatosis syndrome (HFS), a multisystem connective tissue disorder. We show that ANTXR2 is expressed predominantly by fibro-adipogenic progenitors (FAPs) and mediates internalization of the major ECM component collagen VI (COL6). ANTXR2-null (Antxr2-/-) mice exhibit premature COL6 accumulation within intramuscular connective tissue, leading to tissue stiffening and progressive, maladaptive muscle hypertrophy with impaired locomotion and myopathic features. Using Antxr2-/-::Col6a1-/- double-knockout mice, we demonstrate that these phenotypes are COL6-dependent, highlighting the essential role of ANTXR2-mediated COL6 remodeling in preserving ECM homeostasis and skeletal muscle function and establishing a mechanistic basis for muscle pathology in HFS.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 11","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149193941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid remodeling enables adaptation to chronic hyperosmotic stress. 脂质重塑能够适应慢性高渗应激。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-11-02 Epub Date: 2026-10-08 DOI: 10.1083/jcb.202603154

Thomas D Williams, Christian Klose, Robert Ernst, Pedro Carvalho

引用次数: 0

Avl9 defines a family of DENN domain GTPase-activating proteins. Avl9定义了一个DENN结构域gtpase激活蛋白家族。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-11-02 Epub Date: 2026-09-09 DOI: 10.1083/jcb.202601003

Ryan C Vignogna, J Christopher Fromme

引用次数: 0

The Ral small GTPase is an essential regulator of exocyst complex function in secretion 小GTPase是胞囊复合体分泌功能的重要调节因子

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2026-10-06 DOI: 10.1083/jcb.202508181

You Wu,David J. Reiner

{"title":"The Ral small GTPase is an essential regulator of exocyst complex function in secretion","authors":"You Wu,David J. Reiner","doi":"10.1083/jcb.202508181","DOIUrl":"https://doi.org/10.1083/jcb.202508181","url":null,"abstract":"Ral GTPases have long been proposed as regulators of the metazoan exocyst, a conserved secretory vesicle-tethering complex, but direct evidence for this role has been scarce. In contrast, the well-studied yeast exocyst relies on multiple Rab GTPases to regulate function, but yeast do not encode Ral. Using Caenorhabditis elegans, we demonstrate that endogenous RAL-1 directly engages the exocyst through conserved binding sites in its subunits. Loss of RAL-1 disrupts dendritic arborization of PVD sensory neurons, impairs vesicle trafficking, and causes broad developmental defects, acting both cell autonomously in neurons and non-autonomously through supporting epithelial cells. Structure-guided genome editing of RAL-1–exocyst interfaces produced synthetic phenotypes, underscoring the physiological importance of these contacts. Taken together, our findings establish RAL-1 as a bona fide regulator of the metazoan exocyst in vivo and suggest that Ral–exocyst interactions operate in parallel with other secretory pathways. More broadly, this work positions C. elegans as a powerful system to dissect Ral–exocyst mechanisms across molecular, cellular, and developmental scales.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"284 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ana1/Cep295 regulates centriole elongation via Cep135 and microtubules. Ana1/Cep295通过Cep135和微管调控中心粒伸长。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-07-30 DOI: 10.1083/jcb.202504094

Zhenjie Wang, Yuxuan Qian, Xuan Wang, Qianyu Ng, Hanxi Zhang, Yang Wu, Zhen Liu, Zhe Feng

引用次数: 0

Differential contributions of β-tubulin isotypes to acentrosomal oocyte meiosis in C. elegans. β-微管蛋白同型对秀丽隐杆线虫无丝胞体卵母细胞减数分裂的差异贡献。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-08-10 DOI: 10.1083/jcb.202511199

Emmanuel T Nsamba, Anne M Villeneuve

{"title":"Differential contributions of β-tubulin isotypes to acentrosomal oocyte meiosis in C. elegans.","authors":"Emmanuel T Nsamba, Anne M Villeneuve","doi":"10.1083/jcb.202511199","DOIUrl":"10.1083/jcb.202511199","url":null,"abstract":"Oocyte meiotic spindles must achieve bipolarity and segregate chromosomes in the absence of centrosomes. Here, we use high-resolution immunofluorescence microscopy and live imaging to investigate the differential contributions of β-tubulin isotypes (TBB-1 and TBB-2) to assembly and function of acentrosomal spindles in Caenorhabditis elegans oocytes. By combining strains with altered β-tubulin isotype composition with mutations affecting microtubule-crosslinking motor KLP-18 and/or mutations affecting katanin-mediated microtubule severing, we show that TBB-1 and TBB-2 make distinct contributions to promoting spindle bipolarity. Further, by measuring multiple spindle features in wild-type and β-tubulin isotype substitution strains, we reveal contributions of isotype composition to spindle morphology, kinetics of anaphase chromosome separation, and maintenance of spindle structural integrity under stress. Together, our data support a model in which β-tubulin isotype composition helps to maintain a balance between microtubule-crosslinking and severing activities during oocyte meiosis. We further propose that this balance is crucial for establishing spindle bipolarity, maintaining spindle structures, and modulating the dynamics of chromosome separation.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13455592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myosin1D directs circumferential F-actin flow to define cell chirality. Myosin1D指导环向f -肌动蛋白流动来定义细胞手性。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-08-25 DOI: 10.1083/jcb.202509125

Asuka Yamaguchi, Takeshi Sasamura, Kohei Yoshimura, Hiroki Taniguchi, Daisuke Kurisu, Takamasa Higashi, Yasuhiko Sato, Florian L Neugebauer, Shuya Ishii, Makito Miyazaki, Mikiko Inaki, Yasuhiro Inoue, Masakazu Akiyama, Takeshi Haraguchi, Kohji Ito, Kenji Matsuno

{"title":"Myosin1D directs circumferential F-actin flow to define cell chirality.","authors":"Asuka Yamaguchi, Takeshi Sasamura, Kohei Yoshimura, Hiroki Taniguchi, Daisuke Kurisu, Takamasa Higashi, Yasuhiko Sato, Florian L Neugebauer, Shuya Ishii, Makito Miyazaki, Mikiko Inaki, Yasuhiro Inoue, Masakazu Akiyama, Takeshi Haraguchi, Kohji Ito, Kenji Matsuno","doi":"10.1083/jcb.202509125","DOIUrl":"10.1083/jcb.202509125","url":null,"abstract":"Eukaryotic cells possess intrinsic chirality in their structure, motility, and intracellular dynamics, known as cell chirality, which contributes to left-right asymmetric morphogenesis. However, the mechanisms underlying its formation remain elusive. In Drosophila, Myosin1D (Myo1D) and Myosin1C (Myo1C), respectively, dictate right-handed or left-handed chirality of the cell shape and body. Here, we report that Myo1D and Myo1C, respectively, directed clockwise and counterclockwise circumferential F-actin flows in Drosophila macrophages collaborating with Myosin2 (Myo2). Furthermore, Myo1C induced a random F-actin flow in an in vitro motility assay, whereas Myo1D triggered the self-organization of the F-actin ring rotating clockwise in the same conditions, implying that Myo1D assembles F-actin into a circular arrangement with barbed-end-to-pointed-end polarity aligned in a specific direction. We propose a model in which Myo1D induces the formation of F-actin structures aligned in such a polarized fashion within macrophages, and Myo2 rotates them clockwise. This model provides a molecular basis for the formation of cell and organ chirality.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When push comes to shove: How chromosomes absorb spindle forces without losing grip. 当推力来推：染色体如何吸收纺锤体力而不失去控制。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-09-07 DOI: 10.1083/jcb.202608030

Geert J P L Kops

引用次数: 0

Super-resolution imaging reveals altered actin architecture at CAR-T cell immune synapses. 超分辨率成像显示CAR-T细胞免疫突触的肌动蛋白结构改变。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-08-05 DOI: 10.1083/jcb.202506119

Olivia P L Dalby, Efstratios Kirtsios, Hale-Seda Radoykova, Cecilia Zaza, Megan D Joseph, Persis J Amrolia, Alice Giustacchini, Sabrina Simoncelli

{"title":"Super-resolution imaging reveals altered actin architecture at CAR-T cell immune synapses.","authors":"Olivia P L Dalby, Efstratios Kirtsios, Hale-Seda Radoykova, Cecilia Zaza, Megan D Joseph, Persis J Amrolia, Alice Giustacchini, Sabrina Simoncelli","doi":"10.1083/jcb.202506119","DOIUrl":"10.1083/jcb.202506119","url":null,"abstract":"The actin cytoskeleton plays a key integrative role in immunological synapse (IS) formation during T cell activation, but how these dynamics are altered in chimeric antigen receptor (CAR)-T cells remains unclear. Here, we used stimulated emission depletion (STED) microscopy to perform the first super-resolution analysis of actin remodeling at the IS in single- and dual (CD19/CD22) CAR-T cells, activated on supported lipid bilayers across different time points. Quantitative imaging reveals that CAR-T cells form structurally distinct synapses to untransduced cells, characterized by reduced actin-depleted regions, fewer actin foci, and persistent microvilli-like protrusions. These features indicate incomplete cytoskeletal contraction and impaired actin network reorganization, leading to partial synapse maturation. Our findings highlight fundamental differences in actin dynamics between CAR- and TCR-mediated signaling and suggest that defective actin remodeling may contribute to unstable synapse formation, altered signaling integration, and dysregulated responses or off-target effects. These insights could inform future CAR-T engineering strategies to enhance safety and efficacy.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13440243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell cycle-dependent translation-mediated turnover of the long noncoding RNA Malat1. 细胞周期依赖的翻译介导的长链非编码RNA Malat1的周转。

IF 6.1 1区生物学

Journal of Cell Biology Pub Date : 2026-10-05 Epub Date: 2026-08-25 DOI: 10.1083/jcb.202512059

Leah M Plasek-Hegde, Yeolhoe Kim, Rahul V Gupta, Emily A Dangelmaier, Sigrid Nachtergaele, Nadya Dimitrova

{"title":"Cell cycle-dependent translation-mediated turnover of the long noncoding RNA Malat1.","authors":"Leah M Plasek-Hegde, Yeolhoe Kim, Rahul V Gupta, Emily A Dangelmaier, Sigrid Nachtergaele, Nadya Dimitrova","doi":"10.1083/jcb.202512059","DOIUrl":"10.1083/jcb.202512059","url":null,"abstract":"Increased abundance of the nuclear long noncoding RNA (lncRNA) Malat1 drives metastatic progression and is a strong predictor of poor patient prognosis. Although the mechanism that stabilizes Malat1 through processing of its 3' terminus is well-characterized, the pathways governing its turnover remain poorly understood. Here, we show that upon exit from mitosis, Malat1 localizes to the cytoplasm, where it is degraded during early G1, resetting its abundance at the start of each cell cycle. Mechanistically, we demonstrate that Malat1 turnover is mediated by a translation- and Smg1-dependent decay pathway and triggered by redundant elements. Importantly, failure to reset Malat1 levels in early G1, due to decay inhibition or in the absence of progression through mitosis, results in Malat1 accumulation. These findings uncover a cell cycle-dependent mechanism that harnesses the translation machinery to regulate Malat1 abundance and identify cancer cell dormancy as a potential mechanism underlying the widespread overexpression of Malat1 in cancer.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"225 10","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13505482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0