Journal of Cell Biology最新文献_第2页

Feedback control of the heat shock response by spatiotemporal regulation of Hsp70. 通过对 Hsp70 的时空调控对热休克反应进行反馈控制。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-12-02 Epub Date: 2024-09-20 DOI: 10.1083/jcb.202401082

Rania Garde, Annisa Dea, Madeline F Herwig, Asif Ali, David Pincus

{"title":"Feedback control of the heat shock response by spatiotemporal regulation of Hsp70.","authors":"Rania Garde, Annisa Dea, Madeline F Herwig, Asif Ali, David Pincus","doi":"10.1083/jcb.202401082","DOIUrl":"10.1083/jcb.202401082","url":null,"abstract":"Cells maintain homeostasis via dynamic regulation of stress response pathways. Stress pathways transiently induce response regulons via negative feedback loops, but the extent to which individual genes provide feedback has not been comprehensively measured for any pathway. Here, we disrupted the induction of each gene in the Saccharomyces cerevisiae heat shock response (HSR) and quantified cell growth and HSR dynamics following heat shock. The screen revealed a core feedback loop governing the expression of the chaperone Hsp70 reinforced by an auxiliary feedback loop controlling Hsp70 subcellular localization. Mathematical modeling and live imaging demonstrated that multiple HSR targets converge to promote Hsp70 nuclear localization via its release from cytosolic condensates. Following ethanol stress, a distinct set of factors similarly converged on Hsp70, suggesting that nonredundant subsets of the HSR regulon confer feedback under different conditions. Flexible spatiotemporal feedback loops may broadly organize stress response regulons and expand their adaptive capacity.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sec23IP recruits VPS13B/COH1 to ER exit site-Golgi interface for tubular ERGIC formation. Sec23IP 将 VPS13B/COH1 募集到 ER 出口位点-高尔基界面，以形成管状 ERGIC。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-12-02 Epub Date: 2024-10-01 DOI: 10.1083/jcb.202402083

Yuanjiao Du, Xinyu Fan, Chunyu Song, Weiping Chang, Juan Xiong, Lin Deng, Wei-Ke Ji

{"title":"Sec23IP recruits VPS13B/COH1 to ER exit site-Golgi interface for tubular ERGIC formation.","authors":"Yuanjiao Du, Xinyu Fan, Chunyu Song, Weiping Chang, Juan Xiong, Lin Deng, Wei-Ke Ji","doi":"10.1083/jcb.202402083","DOIUrl":"10.1083/jcb.202402083","url":null,"abstract":"VPS13B/COH1 is the only known causative factor for Cohen syndrome, an early-onset autosomal recessive developmental disorder with intellectual inability, developmental delay, joint hypermobility, myopia, and facial dysmorphism as common features, but the molecular basis of VPS13B/COH1 in pathogenesis remains largely unclear. Here, we identify Sec23 interacting protein (Sec23IP) at the ER exit site (ERES) as a VPS13B adaptor that recruits VPS13B to ERES-Golgi interfaces. VPS13B interacts directly with Sec23IP via the VPS13 adaptor binding domain (VAB), and the interaction promotes the association between ERES and the Golgi. Disease-associated missense mutations of VPS13B-VAB impair the interaction with Sec23IP. Knockout of VPS13B or Sec23IP blocks the formation of tubular ERGIC, an unconventional cargo carrier that expedites ER-to-Golgi transport. In addition, depletion of VPS13B or Sec23IP delays ER export of procollagen, suggesting a link between procollagen secretion and joint laxity in patients with Cohen disease. Together, our study reveals a crucial role of VPS13B-Sec23IP interaction at the ERES-Golgi interface in the pathogenesis of Cohen syndrome.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface tension-driven sorting of human perilipins on lipid droplets. 由表面张力驱动的脂滴上人类周皮素的分选。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-12-02 Epub Date: 2024-09-19 DOI: 10.1083/jcb.202403064

Ana Rita Dias Araújo, Abdoul Akim Bello, Joëlle Bigay, Céline Franckhauser, Romain Gautier, Julie Cazareth, Dávid Kovács, Frédéric Brau, Nicolas Fuggetta, Alenka Čopič, Bruno Antonny

{"title":"Surface tension-driven sorting of human perilipins on lipid droplets.","authors":"Ana Rita Dias Araújo, Abdoul Akim Bello, Joëlle Bigay, Céline Franckhauser, Romain Gautier, Julie Cazareth, Dávid Kovács, Frédéric Brau, Nicolas Fuggetta, Alenka Čopič, Bruno Antonny","doi":"10.1083/jcb.202403064","DOIUrl":"10.1083/jcb.202403064","url":null,"abstract":"Perilipins (PLINs), the most abundant proteins on lipid droplets (LDs), display similar domain organization including amphipathic helices (AH). However, the five human PLINs bind different LDs, suggesting different modes of interaction. We established a minimal system whereby artificial LDs covered with defined polar lipids were transiently deformed to promote surface tension. Binding of purified PLIN3 and PLIN4 AH was strongly facilitated by tension but was poorly sensitive to phospholipid composition and to the presence of diacylglycerol. Accordingly, LD coverage by PLIN3 increased as phospholipid coverage decreased. In contrast, PLIN1 bound readily to LDs fully covered by phospholipids; PLIN2 showed an intermediate behavior between PLIN1 and PLIN3. In human adipocytes, PLIN3/4 were found in a soluble pool and relocated to LDs upon stimulation of fast triglyceride synthesis, whereas PLIN1 and PLIN2 localized to pre-existing LDs, consistent with the large difference in LD avidity observed in vitro. We conclude that the PLIN repertoire is adapted to handling LDs with different surface properties.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detailing organelle division and segregation in Plasmodium falciparum. 详述恶性疟原虫细胞器的分裂和分离。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-12-02 Epub Date: 2024-11-01 DOI: 10.1083/jcb.202406064

Julie M J Verhoef, Cas Boshoven, Felix Evers, Laura J Akkerman, Barend C A Gijsbrechts, Marga van de Vegte-Bolmer, Geert-Jan van Gemert, Akhil B Vaidya, Taco W A Kooij

{"title":"Detailing organelle division and segregation in Plasmodium falciparum.","authors":"Julie M J Verhoef, Cas Boshoven, Felix Evers, Laura J Akkerman, Barend C A Gijsbrechts, Marga van de Vegte-Bolmer, Geert-Jan van Gemert, Akhil B Vaidya, Taco W A Kooij","doi":"10.1083/jcb.202406064","DOIUrl":"10.1083/jcb.202406064","url":null,"abstract":"The malaria-causing parasite, P. falciparum, replicates through schizogony, a tightly orchestrated process where numerous daughter parasites are formed simultaneously. Proper division and segregation of one-per-cell organelles, like the mitochondrion and apicoplast, are essential, yet remain poorly understood. We developed a new reporter parasite line that allows visualization of the mitochondrion in blood and mosquito stages. Using high-resolution 3D imaging, we found that the mitochondrion orients in a cartwheel structure, prior to stepwise, non-geometric division during last-stage schizogony. Analysis of focused ion beam scanning electron microscopy data confirmed these mitochondrial division stages. Furthermore, these data allowed us to elucidate apicoplast division steps, highlighted its close association with the mitochondrion, and showed putative roles of the centriolar plaques in apicoplast segregation. These observations form the foundation for a new detailed mechanistic model of mitochondrial and apicoplast division and segregation during P. falciparum schizogony and pave the way for future studies into the proteins and protein complexes involved in organelle division and segregation.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance. Tip60 介导的 Rheb 乙酰化将棕榈酸与 mTORC1 激活和胰岛素抵抗联系起来。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-12-02 Epub Date: 2024-10-18 DOI: 10.1083/jcb.202309090

Zengqi Zhao, Qiang Chen, Xiaojun Xiang, Weiwei Dai, Wei Fang, Kun Cui, Baolin Li, Qiangde Liu, Yongtao Liu, Yanan Shen, Yueru Li, Wei Xu, Kangsen Mai, Qinghui Ai

{"title":"Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance.","authors":"Zengqi Zhao, Qiang Chen, Xiaojun Xiang, Weiwei Dai, Wei Fang, Kun Cui, Baolin Li, Qiangde Liu, Yongtao Liu, Yanan Shen, Yueru Li, Wei Xu, Kangsen Mai, Qinghui Ai","doi":"10.1083/jcb.202309090","DOIUrl":"10.1083/jcb.202309090","url":null,"abstract":"Excess dietary intake of saturated fatty acids (SFAs) induces glucose intolerance and metabolic disorders. In contrast, unsaturated fatty acids (UFAs) elicit beneficial effects on insulin sensitivity. However, it remains elusive how SFAs and UFAs signal differentially toward insulin signaling to influence glucose homeostasis. Here, using a croaker model, we report that dietary palmitic acid (PA), but not oleic acid or linoleic acid, leads to dysregulation of mTORC1, which provokes systemic insulin resistance. Mechanistically, we show that PA profoundly elevates acetyl-CoA derived from mitochondrial fatty acid β oxidation to intensify Tip60-mediated Rheb acetylation, which triggers mTORC1 activation by promoting the interaction between Rheb and FKBPs. Subsequently, hyperactivation of mTORC1 enhances IRS1 serine phosphorylation and inhibits TFEB-mediated IRS1 transcription, inducing impairment of insulin signaling. Collectively, our results reveal a conserved molecular insight into the mechanism by which Tip60-mediated Rheb acetylation induces mTORC1 activation and insulin resistance under the PA condition, which may provide therapeutic avenues to intervene in the development of T2D.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11489267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organization of a cytoskeletal superstructure in the apical domain of intestinal tuft cells. 肠绒毛细胞顶端结构中的细胞骨架上层结构组织

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-12-02 Epub Date: 2024-10-01 DOI: 10.1083/jcb.202404070

Jennifer B Silverman, Evan E Krystofiak, Leah R Caplan, Ken S Lau, Matthew J Tyska

{"title":"Organization of a cytoskeletal superstructure in the apical domain of intestinal tuft cells.","authors":"Jennifer B Silverman, Evan E Krystofiak, Leah R Caplan, Ken S Lau, Matthew J Tyska","doi":"10.1083/jcb.202404070","DOIUrl":"10.1083/jcb.202404070","url":null,"abstract":"Tuft cells are a rare epithelial cell type that play important roles in sensing and responding to luminal antigens. A defining morphological feature of this lineage is the actin-rich apical \"tuft,\" which contains large fingerlike protrusions. However, details of the cytoskeletal ultrastructure underpinning the tuft, the molecules involved in building this structure, or how it supports tuft cell biology remain unclear. In the context of the small intestine, we found that tuft cell protrusions are supported by long-core bundles that consist of F-actin crosslinked in a parallel and polarized configuration; they also contain a tuft cell-specific complement of actin-binding proteins that exhibit regionalized localization along the bundle axis. Remarkably, in the sub-apical cytoplasm, the array of core actin bundles interdigitates and co-aligns with a highly ordered network of microtubules. The resulting cytoskeletal superstructure is well positioned to support subcellular transport and, in turn, the dynamic sensing functions of the tuft cell that are critical for intestinal homeostasis.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Migratory autolysosome disposal mitigates lysosome damage. 迁移性自溶体处理减轻了溶酶体损伤。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-12-02 Epub Date: 2024-09-30 DOI: 10.1083/jcb.202403195

Takami Sho, Ying Li, Haifeng Jiao, Li Yu

引用次数: 0

Mitochondrial-derived compartments are multilamellar domains that encase membrane cargo and cytosol. 线粒体源性区室是一个多纤层结构域，可容纳膜货物和细胞膜。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-11-04 Epub Date: 2024-08-13 DOI: 10.1083/jcb.202307035

Zachary N Wilson, Matt West, Alyssa M English, Greg Odorizzi, Adam L Hughes

{"title":"Mitochondrial-derived compartments are multilamellar domains that encase membrane cargo and cytosol.","authors":"Zachary N Wilson, Matt West, Alyssa M English, Greg Odorizzi, Adam L Hughes","doi":"10.1083/jcb.202307035","DOIUrl":"10.1083/jcb.202307035","url":null,"abstract":"Preserving the health of the mitochondrial network is critical to cell viability and longevity. To do so, mitochondria employ several membrane remodeling mechanisms, including the formation of mitochondrial-derived vesicles (MDVs) and compartments (MDCs) to selectively remove portions of the organelle. In contrast to well-characterized MDVs, the distinguishing features of MDC formation and composition remain unclear. Here, we used electron tomography to observe that MDCs form as large, multilamellar domains that generate concentric spherical compartments emerging from mitochondrial tubules at ER-mitochondria contact sites. Time-lapse fluorescence microscopy of MDC biogenesis revealed that mitochondrial membrane extensions repeatedly elongate, coalesce, and invaginate to form these compartments that encase multiple layers of membrane. As such, MDCs strongly sequester portions of the outer mitochondrial membrane, securing membrane cargo into a protected domain, while also enclosing cytosolic material within the MDC lumen. Collectively, our results provide a model for MDC formation and describe key features that distinguish MDCs from other previously identified mitochondrial structures and cargo-sorting domains.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11320809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell-cell contacts. 脂质转运 ER 蛋白 TMEM24 和 C2CD2 与带 4.1 在细胞-细胞接触处的复合物。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-11-04 Epub Date: 2024-08-19 DOI: 10.1083/jcb.202311137

Ben Johnson, Maria Iuliano, TuKiet T Lam, Thomas Biederer, Pietro V De Camilli

引用次数: 0

Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses. 细胞周期蛋白 B3 是一种显性快速作用细胞周期蛋白，它能驱动早期胚胎的快速有丝分裂。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2024-11-04 Epub Date: 2024-08-06 DOI: 10.1083/jcb.202308034

Pablo Lara-Gonzalez, Smriti Variyar, Shabnam Moghareh, Anh Cao Ngoc Nguyen, Amrutha Kizhedathu, Jacqueline Budrewicz, Aleesa Schlientz, Neha Varshney, Andrew Bellaart, Karen Oegema, Lee Bardwell, Arshad Desai

{"title":"Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses.","authors":"Pablo Lara-Gonzalez, Smriti Variyar, Shabnam Moghareh, Anh Cao Ngoc Nguyen, Amrutha Kizhedathu, Jacqueline Budrewicz, Aleesa Schlientz, Neha Varshney, Andrew Bellaart, Karen Oegema, Lee Bardwell, Arshad Desai","doi":"10.1083/jcb.202308034","DOIUrl":"10.1083/jcb.202308034","url":null,"abstract":"Mitosis in early embryos often proceeds at a rapid pace, but how this pace is achieved is not understood. Here, we show that cyclin B3 is the dominant driver of rapid embryonic mitoses in the C. elegans embryo. Cyclins B1 and B2 support slow mitosis (NEBD to anaphase ∼600 s), but the presence of cyclin B3 dominantly drives the approximately threefold faster mitosis observed in wildtype. Multiple mitotic events are slowed down in cyclin B1 and B2-driven mitosis, and cyclin B3-associated Cdk1 H1 kinase activity is ∼25-fold more active than cyclin B1-associated Cdk1. Addition of cyclin B1 to fast cyclin B3-only mitosis introduces an ∼60-s delay between completion of chromosome alignment and anaphase onset; this delay, which is important for segregation fidelity, is dependent on inhibitory phosphorylation of the anaphase activator Cdc20. Thus, cyclin B3 dominance, coupled to a cyclin B1-dependent delay that acts via Cdc20 phosphorylation, sets the rapid pace and ensures mitotic fidelity in the early C. elegans embryo.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11303871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0